A comparative study of extraction techniques for maximum recovery of β-galactosidase from the yogurt bacterium Lactobacillus delbrueckii ssp. bulgaricus

2020 ◽  
Vol 87 (1) ◽  
pp. 123-126 ◽  
Author(s):  
Rabin Gyawali ◽  
Ayowole Oyeniran ◽  
Tahl Zimmerman ◽  
Sulaiman O. Aljaloud ◽  
Albert Krastanov ◽  
...  
Keyword(s):  
Extraction Method ◽  
Polyacrylamide Gel Electrophoresis ◽  
Extraction Methods ◽  
Maximum Amount ◽  
Lactobacillus Delbrueckii ◽  
Extraction Techniques ◽  
Protein Determination ◽  
Assisted Extraction ◽  
Mechanical Methods ◽  
Mechanical Extraction

AbstractThe study reported in this research communication evaluates the chemical (solvents) and mechanical (sonication, bead-beater) extraction methods to determine the maximum recovery of β-galactosidase from L. bulgaricus spp. Among all extraction techniques, sonication-assisted extraction yielded the highest amounts of enzyme activity (between 1892–2156 Miller Units) in cell-free extract (supernatant). Interestingly, solvent extracted enzyme activities were found to be very low (between 83–153 Miller Units) in supernatant. SDS-polyacrylamide gel electrophoresis and the total protein determination showed that mechanical methods can completely lyse the cells. Our results thus demonstrated that the mechanical extraction method of sonication is the best one for recovering the maximum amount of lactase from L. bulgaricus strains.

scholarly journals Integrated Metabolomics and Transcriptomics Using an Optimised Dual Extraction Process to Study Human Brain Cancer Cells and Tissues

Metabolites ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 240
Author(s):  
Alison Woodward ◽  
Alina Pandele ◽  
Salah Abdelrazig ◽  
Catherine A. Ortori ◽  
Iqbal Khan ◽  
...  
Keyword(s):  
Metabolic Pathways ◽  
Extraction Method ◽  
Limit Of Detection ◽  
Rna Extraction ◽  
Extraction Process ◽  
Extraction Methods ◽  
Serum Starvation ◽  
Extraction Techniques ◽  
Metabolic Genes ◽  
Dual Extraction

The integration of untargeted metabolomics and transcriptomics from the same population of cells or tissue enhances the confidence in the identified metabolic pathways and understanding of the enzyme–metabolite relationship. Here, we optimised a simultaneous extraction method of metabolites/lipids and RNA from ependymoma cells (BXD-1425). Relative to established RNA (mirVana kit) or metabolite (sequential solvent addition and shaking) single extraction methods, four dual-extraction techniques were evaluated and compared (methanol:water:chloroform ratios): cryomill/mirVana (1:1:2); cryomill-wash/Econospin (5:1:2); rotation/phenol-chloroform (9:10:1); Sequential/mirVana (1:1:3). All methods extracted the same metabolites, yet rotation/phenol-chloroform did not extract lipids. Cryomill/mirVana and sequential/mirVana recovered the highest amounts of RNA, at 70 and 68% of that recovered with mirVana kit alone. sequential/mirVana, involving RNA extraction from the interphase of our established sequential solvent addition and shaking metabolomics-lipidomics extraction method, was the most efficient approach overall. Sequential/mirVana was applied to study a) the biological effect caused by acute serum starvation in BXD-1425 cells and b) primary ependymoma tumour tissue. We found (a) 64 differentially abundant metabolites and 28 differentially expressed metabolic genes, discovering four gene-metabolite interactions, and (b) all metabolites and 62% lipids were above the limit of detection, and RNA yield was sufficient for transcriptomics, in just 10 mg of tissue.

scholarly journals Comparison of Different Extraction Methods for the Recovery of Olive Leaves Polyphenols

Processes ◽  
2020 ◽  
Vol 8 (9) ◽  
pp. 1008 ◽  
Author(s):  
Ana Dobrinčić ◽  
Maja Repajić ◽  
Ivona Elez Garofulić ◽  
Lucija Tuđen ◽  
Verica Dragović-Uzelac ◽  
...  
Keyword(s):  
High Pressure ◽  
Cell Wall ◽  
Chlorogenic Acid ◽  
Extraction Efficiency ◽  
Extraction Methods ◽  
Extraction Techniques ◽  
Olive Leaves ◽  
Total Polyphenols ◽  
Assisted Extraction ◽  
Cell Wall Disruption

In the present study, advanced extraction techniques, microwave (MAE), ultrasound (UAE), and high pressure (HPAE)-assisted extraction, were applied to improve extraction efficiency of olive (Olea europaea L.) leaves polyphenols. The effect of sample mass (1.5 and 3 g), MAE—time (2, 8.5, and 15 min) and temperature (45 and 80 °C), UAE—time (7, 14, and 21 min) and amplitude (50 and 100%) and HPAE—time (1, 5.5, and 10 min) and pressure (300 and 500 MPa) on the concentration of each analyzed polyphenol compound was examined. Identified polyphenols were oleuropein, hydroxytyrosol, chlorogenic acid, caffeic acid, verbascoside, and rutin. All three advanced extraction techniques yielded higher content of total polyphenols when compared to the conventional heat-reflux extraction (CE) along with a significant reduction of extraction time from 60 (CE) to 2, 21, and 5.5 min in MAE, UAE, and HPAE, respectively. The most intensive values of tested parameters in each technique were the ones that promoted cell wall disruption, e.g., temperature of 80 °C in MAE, 100% amplitude in UAE and 500 MPa in HPAE. MAE and UAE were more efficient in total polyphenols’ recovery than HPAE.

scholarly journals Anti-infective efficacy of Psidium guajava L. leaves against certain pathogenic bacteria

F1000Research ◽  
2019 ◽  
Vol 8 ◽  
pp. 12
Author(s):  
Pooja Patel ◽  
Chinmayi Joshi ◽  
Tannaz Birdi ◽  
Vijay Kothari
Keyword(s):  
Extraction Method ◽  
Pathogenic Bacteria ◽  
Water Extract ◽  
Psidium Guajava ◽  
Extraction Methods ◽  
Hydroalcoholic Extract ◽  
C Elegans ◽  
Microwave Assisted ◽  
Assisted Extraction ◽  
Infective Activity

Water extracts of Psidium guajava leaves prepared by three different extraction methods were compared with respect to their anti-infective activity against Pseudomonas aeruginosa and Staphylococcus aureus in the nematode host Caenorhabditis elegans. The water extract prepared by Microwave Assisted Extraction method was found to have better anti-infective activity, and its activity was further compared with hydroalcoholic extract prepared using the same extraction method against five different pathogenic bacteria. Both these extracts could attenuate virulence of P. aeruginosa, S. aureus, Serratia marcescens, and Chromobacterium violaceum, towards C. elegans. Anti-infective efficacy of P. guajava leaf extract seems partly to stem from its quorum-modulatory property, as it could modulate production of quorum sensing-regulated pigments in all the susceptible bacteria.

Comparison of Different Extraction Methods for Antioxidant Property of Flavonoids from Pomelo Peel

2013 ◽  
Vol 652-654 ◽  
pp. 443-448 ◽  
Author(s):  
Qiao Mei Ru ◽  
Ru Fan Cai ◽  
Jin Zhe He
Keyword(s):  
Extraction Method ◽  
Antioxidant Activities ◽  
Soxhlet Extraction ◽  
Antioxidant Property ◽  
Extraction Methods ◽  
Antioxidant Ability ◽  
Microwave Assisted ◽  
Pomelo Peel ◽  
Assisted Extraction ◽  
Different Levels

Antioxidant activity of the flavonoids from pomelo (Citrus grandis (L.) Osbeck) peel obtained by conventional soxhlet extraction (CSE), microwave-assisted extraction (MAE) and supercritical carbon dioxide (SC-CO2) extraction were investigated. For measuring antioxidant activities, different methods have been used corresponding to different levels of antioxidant action. The results indicated that the extraction yields of flavonoids by MAE and SC-CO2 extraction were higher than that by CSE method, whereas flavoniods by SC-CO2 extraction exhibited the strongest antioxidant activities. Our results indicated that SC-CO2 extraction method could effectively reserve the antioxidant ability of pomelo peel flavonoids.

scholarly journals Optimalisasi Teknik Ekstraksi dan Isolasi DNA Tanaman Suren (Toona Sureni Merr.) untuk Analisis Keragaman Genetik berdasarkan Random Amplified Polymorphic DNA (RAPD)

2012 ◽  
Vol 14 (1) ◽  
pp. 138 ◽  
Author(s):  
Muh Restu ◽  
Mukrimin Mukrimin ◽  
Gusmiaty Gusmiaty
Keyword(s):  
Genomic Dna ◽  
Extraction Method ◽  
Dna Isolation ◽  
Random Amplified Polymorphic Dna ◽  
Pcr Amplification ◽  
Secondary Compounds ◽  
Extraction Methods ◽  
Extraction Techniques ◽  
High Quality ◽  
Optimum Extraction

The species of trees have different secondary compounds that need optimum extraction techniques. Appropriate extraction techniquesdetermine the quality and quantity of DNA produced. This research aims to found optimal of extraction methods and DNA isolation, thento created genome DNA in high quality and quantity, so that it can be using for genetic variation analyses in Suren (Toona sureni Merr.) byRandom Amplified Polymorphic DNA (RAPD). This study shows that DNA concentrates were 763.3 μg/ml, 180.0 μg/ml, 383.3 μg/ml, and436.7 μg/ml. While based on the results of PCR amplification using the primers OPD 03 shows that the four extraction methods used, the extraction method of number 3 has been able to produce genomic DNA with better quality and more number of bands, although the quantityis lower.

scholarly journals The Effect of Extraction Methods on Preliminary Structural Properties and Antioxidant Activities of Polysaccharides from Lactarius vividus

Processes ◽  
2019 ◽  
Vol 7 (8) ◽  
pp. 482 ◽  
Author(s):  
Zhou Xu ◽  
Shiling Feng ◽  
Jipeng Qu ◽  
Ming Yuan ◽  
Ruiwu Yang ◽  
...  
Keyword(s):  
Structural Properties ◽  
Extraction Method ◽  
Metal Ion ◽  
High Performance ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Extraction Methods ◽  
Hot Water ◽  
Composition Analysis ◽  
Assisted Extraction

Four polysaccharides (LVP-u, LVP-m, LVP-e, and LVP-h) were extracted from the fruiting bodies of Lactarius vividus by an ultrasonic-assisted extraction method, microwave-assisted extraction method, enzyme-assisted extraction method, and hot water extraction method, respectively. Then, the effect of extraction methods on yields, preliminary structural properties, and antioxidant activities was systematically investigated using the weighing method, chemical composition analysis, high-performance gel permeation chromatography (HPGPC), high-performance liquid chromatography (HPLC), Fourier-transform infrared spectroscopy (FT-IR), scanning electron microscope (SEM), radical scavenging, and metal ion chelating assays. Results demonstrated that the four L. vividus polysaccharides (LVPs) were all combined with protein, and the yield of LVP-u was higher than others. Molecular weight distribution, monosaccharide and amino acid compositions, and microstructures among the four LVPs were significantly different. Moreover, the LVPs showed significant antioxidant activities in a dose-dependent manner, and LVP-e demonstrated better antioxidant activities in superoxide anion radical scavenging activity assays and metal ion chelating activity assays, while LVP-u showed higher activity in its hydroxyl radical scavenging ability.

scholarly journals Ultrasonic Thermal-Assisted Extraction of Phosvitin from Egg Yolk and Evaluation of Its Properties

Polymers ◽  
2019 ◽  
Vol 11 (8) ◽  
pp. 1353 ◽  
Author(s):  
Bin Jiang ◽  
Linlin Wang ◽  
Xiaojing Wang ◽  
Shuang Wu ◽  
Dongmei Li ◽  
...  
Keyword(s):  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Egg Yolk ◽  
Extraction Methods ◽  
Natural Food ◽  
Ionization Mass ◽  
Ultrasonic Power ◽  
Assisted Extraction ◽  
Ultrasonic Time ◽  
Significant Difference

Phosvitin (Pv) is the principal phosphoprotein in chicken egg yolk and the most highly phosphorylated protein in nature. Pv is a good natural food antioxidant and emulsifier. However, the current extraction methods present disadvantages of complicated operation and are time-consuming. In this paper, Pv was extracted from the egg yolk by ultrasonic thermal-assisted extraction (UTAE). The effects of heating time, ultrasonic power and ultrasonic time on the extraction of Pv were investigated by a single factor. The purity of Pv, ratio of nitrogen to phosphorus (N/P), and activity were used as evaluation indexes. An efficient extraction of Pv was achieved when the sample was heated for 15 min at 80 °C and then processed for 10 min of ultrasonic treatment with an ultrasonic power of 600 W. Under optimal conditions, the purity and activity of Pv were 80% and 98%, respectively, whereas the ratio of N/P was 3.1. The obtained Pv was identified by sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE), Fluorescence analyses, fourier-transform infrared (FT-IR), and liquid chromatography-nanoelectrospray ionization mass spectrometry (Nano LC-ESI-MS/MS) analysis. The results showed there is no significant difference in the properties of Pv obtained by UTAE and Pv standard. The developed extraction approach is a simple, industrial compatible method without the use of any organic solvents.

scholarly journals Flavonol Composition and Antioxidant Activity of Onions (Allium cepa L.) Based on the Development of New Analytical Ultrasound-Assisted Extraction Methods

Antioxidants ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 273
Author(s):  
Ana V. González-de-Peredo ◽  
Mercedes Vázquez-Espinosa ◽  
Estrella Espada-Bellido ◽  
Ceferino Carrera ◽  
Marta Ferreiro-González ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Extraction Method ◽  
Volume Ratio ◽  
Extraction Methods ◽  
Ultrasound Assisted Extraction ◽  
Quercetin Derivatives ◽  
Extraction Solvent ◽  
Assisted Extraction ◽  
Response Optimization ◽  
Ultrasound Assisted

The onion is one of the most cultivated and consumed vegetables, and is a very valuable source of antioxidant substances. Every onion variety is rich in flavonols—mainly quercetin derivatives which makes onions the main dietary source of these compounds. This study intends to develop an ultrasound-assisted extraction method (UAE), an economical, green, and efficient technique, that allows us to determine the quality of onion extracts in terms of flavonol composition and antioxidant activity. For this purpose, an ultrasound-assisted extraction method has been optimized to obtain extracts with a high amount of flavonols, as well as with a high antioxidant activity, not only separately using a Box–Behnken design, but also simultaneously, based on multi-response optimization. Multiple response optimization has not only demonstrated an efficacy level similar to that of the individual ones, but it also represents a considerable reduction in terms of cost, time and work. The optimal conditions for simultaneous extractions were determined as follows: 76.8% methanol as an extraction solvent at pH 2 and 58.5 °C temperature, 85% amplitude, 0.9 s cycle, and 0.2:13 g:mL sample mass/solvent volume ratio. Furthermore, the developed method exhibited a high precision level and great recoveries in a rather short extraction time (2 min). These results, together with the development of a fast and simple UHPLC analysis method, lead us to consider the developed UAE method as a suitable technique for the extraction of bioactive compounds from onion matrices.

scholarly journals PENGARUH METODE EKSTRAKSI TERHADAP KADAR TOTAL FENOLIK, KADAR TOTAL FLAVONOID DAN AKTIVITAS ANTIOKSIDAN EKSTRAK DAUN MENGKUDU (Morinda citrifolia L.)

2021 ◽  
Vol 18 (01) ◽  
pp. 40
Author(s):  
Septiana Laksmi Ramayani ◽  
Eka Amalia Permatasari ◽  
Indah Novitasari ◽  
Maryana Maryana
Keyword(s):  
Antioxidant Activity ◽  
Extraction Method ◽  
Leaf Extract ◽  
Extraction Methods ◽  
Total Flavonoid ◽  
Morinda Citrifolia ◽  
Antihypertensive Activity ◽  
Total Phenolic ◽  
Hypertension Prevention ◽  
Assisted Extraction

ABSTRACT Excessive Reactive Oxygen Species (ROS) can cause hypertension. Prevention of the risk of hypertension is consume an antioxidant. Noni (Morinda citrifolia L). is a plant that empirically proven to lower blood pressure and has antioxidant activity. Antihypertensive activity of noni leaves is influenced by phenolic and flavonoid compounds. The extraction method affects the levels of compound in extracts. The aims of study is determine the effect of the extraction method on the total phenolic level, total flavonoid level and antioxidant activity of noni leaf extract. The extraction methods are maceration, Microwave Assisted Extraction and Soxhlet. The results showed that the different extraction methods affected the total phenol level, total flavonoid level and antioxidant activity of the noni leaf extract. The Soxlet method provides the highest total phenolic level, total flavonoid level and antioxidant activity than other extraction methods. Keywords: Noni leaf extract, extraction method, total phenolic level, total flavonoid level and antioxidant activity

scholarly journals Optimization of Extraction Conditions for Determination of Tiliroside in Tilia L. Flowers Using an LC-ESI-MS/MS Method

2019 ◽  
Vol 2019 ◽  
pp. 1-9
Author(s):  
Aleksandra Pieczykolan ◽  
Wioleta Pietrzak ◽  
Renata Nowak ◽  
Józefina Pielczyk ◽  
Karolina Łamacz
Keyword(s):  
Solvent Extraction ◽  
High Efficiency ◽  
Wide Spectrum ◽  
Extraction Methods ◽  
Accelerated Solvent Extraction ◽  
Extraction Techniques ◽  
Ultrasound Assisted Extraction ◽  
Considerable Saving ◽  
Esi Ms ◽  
Assisted Extraction

Tiliroside exhibits a wide spectrum of effects on the human body; considering expensive synthesis of tiliroside, linden trees seem to be a good source of this compound. For the first time, 46 various extraction methods were developed to receive tiliroside from Tilia L., including ultrasound-assisted extraction, maceration, maceration with stirring, accelerated solvent extraction, and extraction under reflux. The effects of extraction techniques, solvents, additives, and temperature on the content of tiliroside were studied using analytical and statistical methods. A new, rapid, simple, sensitive, and selective liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS) method was developed to determine the content of tiliroside in Tilia L. flowers. The LC-ESI-MS/MS analysis demonstrated the highest content of tiliroside in Tilia L. flowers obtained using accelerated solvent extraction (ASE) where 70% ethanol with addition of 1% acetic acid was used as a solvent (7.400 ± 0.019 mg of tiliroside per g dry extract).The results showed that the extracts of Tiliae inflorescentia contained large amounts of tiliroside; therefore, they are good sources of this compound. Moreover, ASE was found to be superior to other extraction techniques due to its high efficiency as well as considerable saving of time and solvent.

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