Dietary choline requirement of juvenile grass shrimp (Penaeus monodon)

2001 ◽  
Vol 72 (3) ◽  
pp. 477-482 ◽  
Author(s):  
S. Y. Shiau ◽  
P. S. Lo

AbstractA feeding trial was conducted to determine the dietary choline requirement of juvenile grass shrimp, Penaeus monodon. Purified basal diets were formulated using vitamin-free casein (containing 370 mg choline per kg) as the protein source. Graded levels (0, 300, 600, 1000, 2000, 4000, 7000, and 10 000 mg choline per kg diet) of choline chloride were added to the basal diet, resulting in eight dietary treatments in the experiment. Each diet was given to three replicate groups of shrimp initially averaging 1·18 (s.d. 0·01) g for 8 weeks. Weight gain was highest in shrimp given the diets supplemented with 7000 and 4000 mg choline per kg diet, followed by the groups given 2000 mg/kg, then 1000 and 600 mg/kg, and finally 300 mg/kg and the unsupplemented control group (P < 0·05). Shrimp given diets supplemented with 7000 and 4000 mg choline per kg diet had significantly higher food efficiency and survival than those given diet with 300 mg choline per kg or the control diet. Higher body crude protein and choline concentrations were recorded in shrimp given diets supplemented with 4000 and 7000 mg choline per kg diet than shrimp given diets with ≤600 mg choline/kg and ≤2000 mg choline per kg, respectively. Analysis by polynomial regression of weight gain and body choline concentration for P. monodon gave a maximum at about 6000 mg/kg. Taking into account the choline concentration of the unsupplemented basal diet, the optimal dietary choline requirement for growing P. monodon is about 6200 mg/kg.

2002 ◽  
Vol 75 (1) ◽  
pp. 97-102 ◽  
Author(s):  
S. Y. Shiau ◽  
W. H. Cho

AbstractA feeding trial was conducted to determine the adequate level of dietary choline for juvenile grass shrimp, Penaeus monodon, given diets containing different dietary lipid concentrations. Purified diets with six levels of choline chloride (0, 2000, 5000, 8000, 11000 and 14000 mg/kg diet) at either 50 or 110 g/kg lipid concentration were each given to three replicate groups of 15 shrimp (mean weight: 0·88 (s.d. 001) g) for 8 weeks. Results indicated that in the 50 g/kg lipid group, weight gain was highest in shrimp given the diets supplemented with 5000 mg choline per kg diet, intermediate in the group given 8000 mg choline per kg diet and lowest in the 14000 mg choline per kg diet and the unsupplemented control groups (P < 005); food efficiency (FE) was highest in shrimp given 5000 mg choline per kg diet, followed by the groups given > 8000 mg choline per kg diet and lowest for the unsupplemented control group. In the 110 g/kg lipid group, weight gain was highest in shrimp given the diets supplemented with 5000 and 8000 mg choline per kg diet, intermediate in the groups given 2000 and 14000 mg choline per kg diet and lowest in the control group; FE was higher in shrimp given 8000 mg choline per kg diet than in any of the other dietary groups; hepatic lipid concentration was higher in shrimp given < 2000 mg choline per kg diet than shrimp given > 5000 mg choline per kg diet. In both lipid groups, shrimp given < 2000 mg choline per kg diet had higher hepatosomatic index (HSI) than shrimp given > 5000 mg choline per kg diet. Of shrimp given <2000 mg choline per kg diet, those in the 110 g/kg lipid group had higher HSI and hepatic lipid concentration than those in the 50 g/kg lipid group. Analysis by polynomial regression of weight gain indicated that the optimum dietary choline requirements in juvenile grass shrimp were about 6400 mg/kg diet and 7800 mg/kg diet in 50 and 110 g/kg lipid diets, respectively.


2004 ◽  
Vol 91 (4) ◽  
pp. 585-590 ◽  
Author(s):  
Shi-Yen Shiau ◽  
Li-Shan Lu

Two 8-week feeding trials were conducted to determine the dietary Na requirement for juvenile hybrid tilapia (Oreochromis niloticus × O. aureus) reared in fresh water and seawater. In each experiment, NaCl was added to the basal diet at 0, 0·5, 1, 2, 3, 5, or 7g Na/kg diet (fresh water) and at 0, 0·2, 0·5, 0·8, 1·2, 1·5, 2, or 3g Na/kg diet (seawater). Each diet was fed to three replicate groups of fish, individual fish initially weighing 0·69 (se 0·01) g, in a closed, recirculating rearing system. In fresh water, the tilapia fed the diet supplemented with 2g Na/kg diet had significantly (P<0·05) greater weight gain than the fish fed the diets supplemented with ≥3 and ≤0·5g Na/kg diet. Feed efficiency (FE) in fish generally followed the weight-gain pattern. Gill Na+–K+ ATPase activity was highest in the fish fed the diets supplemented with 1–3g Na/kg diet, followed by the fish fed the diet with 7g Na/kg diet and lowest in the fish fed the unsupplemented control diet. In seawater, the weight gain, FE and gill Na+–K+ ATPase activity in fish were not affected by the dietary treatment. Analysis by polynomial regression of weight gain, by broken-line regression of gill Na+–K+ ATPase activity and by linear regression of whole-body Na retention of the fish reared in fresh water, indicated that the adequate dietary Na concentration for tilapia is about 1·5g/kg diet. The present study also suggests that no dietary Na is required for tilapia reared in seawater.


2020 ◽  
Vol 46 (1) ◽  
pp. 61-70
Author(s):  
R. Olajide ◽  
E. K. Asaniyan ◽  
S. M. Aro ◽  
O. B. Olusegun

Sixty (60) grower-finisher pigs (Landrace x Large white) were used to investigate the nutritional value of beniseed hull (BSH) as substitute for maize on performance, carcass, and organs characteristics of the experimental animals. Five experimental diets were formulated by incorporating BSH into basal diet to replace maize at 0, 25, 50, 75 and 100%; designated as T1 (control), T2, T3, T4 and T5, respectively. The experimental pigs were individually weighed and assigned to five dietary treatments of three replicates of four animals each in a completely randomized design. The animals were fed for 2 weeks pre-experimental period and thereafter for ten weeks during which data were collected. Feed cost per kg live weight gain (? 668.67) of the animals fed 0% BSH significantly (P<0.05) reduced to ? 620.06 (25% BSH), ? 527.24 (50% BSH), ? 509.74 (75% BSH) and ? 456.73 (100% BSH). The bled weight, scald weight, eviscerated weight, dressing percentage, bacon, fore hock, hind hock and tail were significantly (P<0.05) affected by dietary treatments. The biggest liver weight (2.27g/kg) obtained in the animals fed the control diet (0% BSH) reduced significantly (P<0.05) to 2.20 (25% BSH), 1.91 (50% BSH), 1.76 (75% BSH) and 2.00 (100% BSH). Variations obtained in the kidney weight (0.40 g/kg) of the experimental animals fed with 25% BSH-based diets were similar (P>0.05) to 0.38 g/kg in the control (0% BSH) with both significantly (P<0.05) reduced to 0.36 (50% BSH), 0.36 (75% BSH) and 0.32 (100% BSH), respectively. Beniseed hull (BSH) poses no health challenge to the pigs used in this study. There were reductions in the cost/kg and cost of feed per kilogram live-weight gain with increasing levels of BSH in the diets, the lowest being at 100% level of substitution; beniseed hull could economically and safely replace maize in pig's diets up to 100% level.


2011 ◽  
Vol 78 (2) ◽  
pp. 144-148 ◽  
Author(s):  
Vincenzo Tufarelli ◽  
Vito Laudadio

The objective of this study was to evaluate the effect of dietary supplementation with selenium (Se) and vitamin E (VitE) on milk yield, composition and renneting parameters in dairy Jonica breed goats. Forty multiparous lactating goats fed the same basal diet were randomly allocated to 1 of 2 dietary treatments: for 135 days, the experimental period. Basal diet was composed by ad libitum mixed vetch-oat hay and a definite amount of a pelleted concentrate. Dietary treatments included: (1) the control diet containing background Se and VitE only, and (2) the experimental diet containing sodium selenite as the Se supplement in concentrate (0·20 mg/head per day of Na-selenite and 20 mg/day per head of VitE). During the trial, no significant differences (P>0·05) were registered in dry matter intake and body weight gain among groups. Goats fed Se-VitE diet showed the highest milk production (P<0·05), and fat (P<0·01) and protein (P<0·05) yields and percentages. Somatic cell count were significantly lower in Se-VitE diet compared with control group (P<0·05). Significant differences (P<0·05) were observed in milk renneting properties. Based on our results, it was concluded that dietary supplementation with SE and VitE led to an increase of milk production and quality in Jonica goat, with positively improvement in milk coagulation aptitude.


Animals ◽  
2018 ◽  
Vol 8 (7) ◽  
pp. 117 ◽  
Author(s):  
Mehdi Qorbanpour ◽  
Taha Fahim ◽  
Faramin Javandel ◽  
Mehran Nosrati ◽  
Erwin Paz ◽  
...  

A total of 225 day-old male broiler chicks (Ross-308) were randomly allocated to five treatment groups, with three replicates in a completely randomized design for 42 days. Birds were fed a basal-diet supplemented with: no additive (control-diet), multi-strain probiotic (Lactobacillus acidophilus, Lactobacillus casei, Enterococcus faecium and Bifidobacterium thermophilum), or 0.15, 0.20 and 0.25% ginger (Z. officinale) powder, respectively. The results show no significant differences among treatments for growth traits and carcass characteristics, whereas using probiotics and ginger at all levels resulted in a significant decrease of gizzard weight and abdominal fat compared to the control group. Dietary treatments did not affect blood biochemistry and antibody production against sheep red blood cells (SRBC), IgG and IgM; however, antibody titre was higher in birds fed 0.25% ginger than other diets after 7 days post injection. The Lactobacillus counts in ileal content of birds fed 0.20 and 0.25% ginger were higher compared to the other treatments. In conclusion, dietary supplementation with either ginger or probiotics showed a significant influence on birds’ immune response, probably because ginger had strong antioxidant activity and the probiotics stimulated the production of natural antibodies.


2019 ◽  
Vol 97 (Supplement_2) ◽  
pp. 80-81
Author(s):  
Lori L Thomas ◽  
Ashley Hartman ◽  
Jason C Woodworth ◽  
Mike D Tokach ◽  
Robert D Goodband ◽  
...  

Abstract A total of 360 pigs (DNA 400×200, initial BW=6.8 kg) were used a 35-d trial to evaluate the effects of adding MCFA and monolaurin blends to the diet on growth performance of nursery pigs. Pens of pigs were blocked by BW and randomized to 1 of 6 dietary treatments (12 pens per treatment). Treatments consisted of a basal diet containing no MCFA (control), the control diet with 1.0% added MCFA (a 1:1:1 blend of C6, C8, and C10; Sigma Aldrich, St. Louis, MO), or a 1.0% inclusion of 4 different blends of MCFA, lactic acid, and monolaurin (1, 2, 3, and 4). The 4 blends consisted of 50% C6, 20% lactic acid and increasing levels of monolaurin (0, 10, 20, and 30%) at the expense of C12 (30, 20, 10, and 0%). Data were analyzed as a randomized complete block design with pen as the experimental unit. From d 0 to 14, pigs fed the 1.0% 1:1:1 MCFA blend had increased (P = 0.037) ADG compared to the control group. Pigs fed the 1.0% 1:1:1 MCFA blend and the mean of the 4 blends of MCFA, lactic acid, and monolaurin had improved (P < 0.037) G:F compared with pigs fed the control diet. From d 14 to 35, ADFI and subsequently ADG increased (P < 0.057) for pigs fed the 1.0% 1:1:1 MCFA blend compared with the control group. Overall, pigs fed the 1.0% 1:1:1 MCFA blend had increased (P < 0.034) ADFI and ADG, resulting in 0.9 kg greater final BW (P = 0.014) compared with the control group. There was no evidence for differences between the mean of pigs fed blends of MCFA, lactic acid, and monolaurin compared with the control. In summary, the addition of a 1.0% 1:1:1 MCFA blend resulted in improved ADG, ADFI, and G:F compared with pigs fed a control diet.


Animals ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 1381
Author(s):  
Tzuen-Rong J Tzeng ◽  
Tzu-Yu Liu ◽  
Chiao-Wei Lin ◽  
Pei-En Chang ◽  
Pei-Xin Liao ◽  
...  

Alternative growth promoters are able to not only effectively replace the traditional use of antibiotics but also provide additional health benefits for livestock and reduce food safety concerns. This study investigated the effects of dry Hydrastis canadensis on the laying performance and fecal microbial community of laying hens. Twenty-four Lohmann (LSL, white layer strain) hens were reared from 40 to 48 weeks of age and randomly allotted to four dietary treatments (six birds/treatment). The dietary treatments comprised a basal diet with no treatment as control, a basal diet plus 0.6% powder of dry Hydrastis canadensis roots (R) or leaves (L), and a basal diet plus 0.6% powder of a mixture of dry Hydrastis canadensis roots and leaves (1:1, LR). No mortality was observed in the whole experimental period. The results indicated that albumen height in the LR group was significantly greater than that in the control group. The diet supplemented with Hydrastis canadensis had no significant effects on egg production rate, egg weight, eggshell strength, eggshell thickness, Haugh unit, or yolk height during the whole experimental phase. However, principal coordinate analysis, comparative heat map analysis, and cluster dendrogram analysis of cecal microbiota showed distinct clusters among the groups treated with Hydrastis canadensis and the control group. Regarding blood biochemical parameters, serum cholesterol levels were significantly lower in all Hydrastis canadensis-treated groups compared with those in the control group. Moreover, serum low-density lipoprotein levels were lower in hens supplemented with the leaf of Hydrastis canadensis. The abundances of the phyla Fusobacteria and Kiritimatiellaeota were increased (p < 0.05) in laying hens fed with 0.6% Hydrastis canadensis leaves, whereas the abundance of the phylum Firmicutes in cecum digesta decreased in response to treatment with Hydrastis canadensis roots and leaves. The relative abundance of the Fusobacterium genus was higher in the LR group compared with that in the control. On the contrary, we found a different trend in the Synergistes genus. The potential influences of these microbiota on the performance of laying hens were discussed. The results demonstrate that Hydrastis canadensis can improve the egg albumen height and modulate the cecum digesta microbiota composition of laying hens.


2001 ◽  
Vol 85 (2) ◽  
pp. 213-218 ◽  
Author(s):  
Shi-Yen Shiau ◽  
Jia-Fen Hsieh

An 8 week feeding trial was conducted to determine the dietary K requirement for juvenile hybrid tilapia (Oreochromis niloticus × O. aureus). Purified diets with eight levels (0, 1, 2, 3, 4, 5, 7, 10 g/kg diet) of supplemental K were fed to tilapia. Each diet was fed to three replicate groups of fish initially weighing a mean value of 0.77 (SE 0.01) g/fish in a closed, recirculating rearing system. Weight gain was higher (P<0.05) in fish fed the diets supplemented with 2, 3 and 4 g K/kg diet than in fish fed diet with 10 g K/kg diet and the unsupplemented control diet. Gill Na+-K+ ATPase activity was highest in fish fed the diets supplemented with 1–3 g K/kg diet, followed by fish fed the diet with 5 g K/kg diet and lowest in fish fed the diet with 10 g K/kg diet. Whole-body K content in fish were generally increased as the dietary K supplementation level increased. Analysis by polynomial regression of weight gain and gill Na+-K+ ATPase activity and by linear regression of whole-body K retention of the fish indicated that the adequate dietary K concentration for tilapia is about 2–3 g/kg diet.


AMB Express ◽  
2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Cong Wang ◽  
Qing Liu ◽  
Fengchun Ye ◽  
Hongbo Tang ◽  
Yanpeng Xiong ◽  
...  

AbstractPurslane is a widespread wild vegetable with both medicinal and edible properties. It is highly appreciated for its high nutritional value and is also considered as a high-quality feed resource for livestock and poultry. In this study, Sanhuang broilers were used to investigate the effect of feeding purslane diets on the growth performance in broilers and their gut microbiota. A total of 48 birds with good growth and uniform weight were selected and randomly allocated to four treatment groups A (control), B, C and D. Dietary treatments were fed with basal diet without purslane and diets containing 1%, 2% and 3% purslane. The 16S rDNA was amplified by PCR and sequenced using the Illumina HiSeq platform to analyze the composition and diversity of gut microbiota in the four sets of samples. The results showed that dietary inclusion of 2% and 3% purslane could significantly improve the growth performance and reduce the feed conversion ratio. Microbial diversity analysis indicated that the composition of gut microbiota of Sanhuang broilers mainly included Gallibacterium, Bacteroides and Escherichia-Shigella, etc. As the content of purslane was increased, the abundance of Lactobacillus increased significantly, and Escherichia-Shigella decreased. LEfSe analysis revealed that Bacteroides_caecigallinarum, Lachnospiraceae, Lactobacillales and Firmicutes had significant differences compared with the control group. PICRUSt analysis revealed bacteria mainly enriched in carbohydrate metabolism pathway due to the additon of purslane in the diet. These results suggest that the addition of purslane to feed could increase the abundance of Lactobacillus in intestine, modulate the environment of gut microbiota and promote the metabolism of carbohydrates to improve its growth performance. This study indicates that the effect of purslane on the growth-promoting performance of broilers might depend on its modulation on gut microbiota, so as to provide a certain scientific basis for the application of purslane in the feed industry.


2019 ◽  
Vol 97 (Supplement_2) ◽  
pp. 141-142
Author(s):  
Jerad R Jaborek ◽  
Alejandro E Relling

Abstract The presence of reactive oxygen species has been associated with the differentiation of pre-adipocytes into lipid filled mature adipocytes in vitro. We hypothesized offering a diet without supplemental antioxidant minerals (Cu, Mg, Zn, and Se) to steers during the growing phase, a time when intramuscular fat cells are believed to be proliferating, would promote differentiation of pre-adipocytes, leading to more desirable marbling scores compared with steers supplemented to meet their mineral requirements. After adaptation to the feedlot, 168 Sim-Angus steers were divided into four weight blocks, placed in one of twenty-four pens, and randomly assigned a dietary treatment. Dietary treatments were: 1) no supplemental (Cu, Mg, Zn, and Se) minerals; 2) control diet with supplemented minerals to meet the mineral requirements of growing beef steers (NRC, 2016); 3) Cu, Mg, Zn, and Se concentrations supplemented at twice the concentration of the control diet. After dietary treatments were applied for the 4-wk long growing phase, steers were offered a common finishing diet until reaching a similar backfat thickness until harvest. Feedlot performance and carcass data were analyzed in SAS with pen as the experimental unit in the following model: Yij = μ+Di+wj+eij, where Di was the fixed effect of diet, wj was the random effect of weight block, and eij was the random error. No significant (P > 0.35) treatment effects were found for feedlot performance and carcass measurements. The feedstuffs in the basal diet may have contained sufficient concentrations of antioxidant minerals to meet the mineral requirements of the steers and mask differences in marbling. Additionally, offering a similar diet during the finishing period may have resulted in compensatory marbling growth, which offset marbling differences after the growing phase and resulted in uniform marbling accumulation across dietary treatments.


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