The Ultrastructure of Cadaverous Skin after Cryopreservation with Polyethylene Oxide-400

2000 ◽  
Vol 6 (S2) ◽  
pp. 592-593
Author(s):  
R. Migunova ◽  
B. Sandomirski ◽  
A. Kaprelyants
Keyword(s):  
Low Temperature ◽  
Polyethylene Oxide ◽  
Low Temperatures ◽  
Tissue Preservation ◽  
Accidental Death ◽  
Ultrastructural Characteristics ◽  
Large Wound ◽  
Graft Skin ◽  
Selection Of

The cryopreservation is the method of selection of long-term skin preservation used for the closure of large wound defects and the only possibility of long preservation of graft skin material in the viable state. For the lowtemperature preservation of human skin was successfully used cryoprotectant Polyethylene oxide -400, which was confirmed by the clinical evaluation during autotransplantation /1/. At the same time many aspects of the influence of low temperatures on the ultrastructural skin components were unclear /2/. The investigation of subcellular elements during step of low-temperature preservation specifies mechanisms of influence on the tissue preservation factors and determine the condition of skin cellular elements /3,4,5/. The aim of this work is to study ultrastructural characteristics of human cadaverous skin during the stages of low-temperature preservation by PEO-400.In this investigation epidermal skin grafts with the thickness of 0.4-0.6 mm were studied in 6 hours after the accidental death divided in 3 series:

Perspectives of the advanced functional using of some ornamental bushy crops

2021 ◽  
pp. 212-222
Author(s):  
V.M. Yezhov ◽  
◽  
V.I. Dubrovskyi ◽  
O.Yu. Ansieiev ◽  
O.A. Olenich ◽  
...  
Keyword(s):  
Low Temperatures ◽  
Left Bank ◽  
New Variety ◽  
Common Lilac ◽  
Significant Yield ◽  
The Common ◽  
Ornamental Shrubs ◽  
The Rose ◽  
Selection Of

The authors present the results of the collection’s formation of some ornamental shrubs, long-term phenological observations of them and the selection of the best forms and breeding hybrids in the Left-Bank Lisosteppe of Ukraine. In particular, the characteristic of the vegetation of 20 varieties of common lilac collection is given. The estimation of the terms and duration of the crop flowering enable to divide its varieties into groups of early, average and late flowering. The high decorative value of most of the strains has been described as well as their resistance to low temperatures and pests. The additional analysis of the latest literature data has shown the significant yield of aromatic substances in the common lilac plant, with the predominance of alkanes and lilac alcohol. The results have been presented of 70 rose cultivars of adaptation to the growing zone conditions. There are 20 promise varieties of the crop, which are characterized by high decorative value, resistance to pathogens and low temperatures and duration of flowering. The characteristics of the new variety of roses of the floribunda group – Olexandra has been given created by the authors. Along side with the high decorative value, different types of the rose plant provide the significant yield of aromatic substances with the domination of alkanes, monoterpenes and 2-phenylethanol. The analysis of the literature data also has shown the presence of aromatic substances in the jasmine and mock-orange plants. The first is characterized with esters based on benzene, cis-jasmon and isophytol, the second – with some sesquiterpenes and epi-13-manol. The further research included the phenological evaluation of the of white cedar and juniper collection. The best selected forms were characterized with decorativeness, resistance to frost, pests and pathogens. The literature data have shown, that the aromatic complex of the crops cultures includes mainly monoterpenes and their derivatives – alcohols and ketones. As a result, it was concluded that it is necessary to expand research with ornamental bushy crops in the direction of evaluating of their aromatic properties.

scholarly journals EFFECTS OF LONG-TERM RELATIVELY HIGH AND LOW TEMPERATURE USE ON GROWTH PERFORMANCE AND MEAT QUALITY OF BROILERS

2020 ◽  
pp. 12-17
Author(s):  
Yingying Qiao ◽  
Oleksandr Kyselov ◽  
Changzhong Liu
Keyword(s):  
High Temperature ◽  
Low Temperature ◽  
Growth Performance ◽  
Meat Quality ◽  
Low Temperatures ◽  
Production Performance ◽  
Control Group ◽  
Temperature Group

The experiment aims to study the effects of long-term relatively high and low temperatures on growth performance and meat quality of broiler chickens. The experiment was carried out in Yunnan Academy of Animal Science, for determine the quality of meat used the laboratories of Henan Institute of Science and Technology. A total of experiment use 240 healthy 1-day-old Avian broiler chickens were randomly divided into three groups: relatively high temperature group, low temperature group and control group. The results of the experiment confirm that at low temperatures, when the energy consumption of the animal decreases, it leads to weight loss, which we can see in the low-temperature group, the average daily weight gain in this experiment was significantly lower than in the control group (P <0.05). It was found that low-temperature stress significantly increased the mortality of broilers, at the age of 42 days in the low-temperature group, the mortality of chickens was higher than in the control group, by 71.4%. Among all evaluated groups on the content of unsaturated fatty acids SFA, PUFA, MUFA and EFA in the muscles of the breasts of broilers, the lowest content was in the lower temperature group than in the control group, by 48.3%, 46.9%, 51.5% and 43.9%. Studies have shown that influence of high-temperature above 30°C causes disturbances in poultry behavior and physiology, leading to reduced production performance. Broilers aged 35-40 days experienced 31°C high-temperature stress and found that their performance and immunity decreased. Broilers feed intake and growth rate at 35°C high temperature were reduced by 13% and 32% than at 20°C. The results showed that: ① Relatively high temperature and low temperature for a long time reduced the growth performance and mortality of broilers, and long-term relatively low temperature decreased the slaughter performance of broilers.② Relatively high and low temperatures for a long period of time reduced the levels of serine, glycine, SFA, PUFA, USFA, EFA and MUFA in broiler breast muscles negative effect on meat quality. ③ The long-term relatively low temperature has a greater adverse effect on broilers than the long-term relatively high temperature. The results provided some theoretical basis for accurately setting the broiler breeding environment temperature, improving broiler quality, maximizing broiler production performance, and increasing the economic benefits of the farm.

Feeling the cold in a warming climate: differential effects of low temperatures on co-occurring eucalypts

2016 ◽  
Vol 64 (5) ◽  
pp. 456
Author(s):  
George Matusick ◽  
Katinka X. Ruthrof ◽  
Jason Pitman ◽  
Giles E. St. J. Hardy
Keyword(s):  
Low Temperature ◽  
Species Composition ◽  
Low Temperatures ◽  
Tree Mortality ◽  
Damage Index ◽  
Extreme Temperature ◽  
Climatic Conditions ◽  
Eucalyptus Marginata ◽  
Repeated Events

Climate change is altering extreme temperature events, and is expected to drive changes in species composition as a result. To assess the potential for compositional shifts from low-temperature events, the effects of repeated events in 2010 and 2012 on three co-occurring eucalypts were determined in south-western Australia. To examine the climatic conditions that led to tree damage, and the long-term pattern of low-temperature events, temperatures were monitored on affected sites, and modelled from climate-station data. The three species varied considerably in their susceptibility to low temperatures. Corymbia calophylla K.D.Hill & L.A.S.Johnson was most affected (crown-damage index (CDI) = 47), followed by Eucalyptus marginata Sm. (CDI = 17) and E. wandoo Blakely (CDI = 3), which was comparatively tolerant. The temperatures leading to damage in 2010 and 2012 were –3.4°C and –2.1°C respectively. The frequency of low-temperature events (days below 0°C) have been steadily increasing in the study area since the mid-1990s. Because minimal tree mortality was observed, species composition is unlikely to change as a result of low temperatures in the short term. However, continued dieback from repeated events may disrupt regenerative processes, and cause long-term compositional shifts.

The impact of low temperatures in controlling the geographical distribution of plants

1990 ◽  
Vol 326 (1237) ◽  
pp. 585-593 ◽  
Keyword(s):  
Seed Germination ◽  
Low Temperature ◽  
Low Temperatures ◽  
Marked Change ◽  
Tilia Cordata ◽  
Natural Range ◽  
Distribution Limits ◽  
The Impact ◽  
Temperature Responses

The distribution limits of three species, in the British Isles are discussed. For Verbena officinalis and Tilia cordata low temperatures are shown to influence distribution, by limiting the capacity either to flower or to fertilize ovules, respectively. In the case of Umbilicus rupestris , a long-term transplant population beyond the natural geographical limit of the species has evolved new low-temperature responses of seed germination and winter survival. The effect is a marked change of phenology, compared with populations of the species within its natural range, which enhances the capacity of the population to survive in a colder environment.

scholarly journals The resistance of plants of Juglans nigra varieties to the influence of the low temperature

2021 ◽  
pp. 255-259
Author(s):  
О. I. Rudnyk-Ivashchenko ◽  
M. V. Swed ◽  
V. V. Sсhwartau
Keyword(s):  
Low Temperature ◽  
Low Temperatures ◽  
Winter Hardiness ◽  
Black Walnut ◽  
Juglans Nigra ◽  
The Core ◽  
Breeding Work ◽  
Structural Parts ◽  
Stable Forms ◽  
Selection Of

The results of research on the influence of low temperatures on the structural parts of shoots of three varieties and a hybrid of domestic and foreign selection of black walnut in the conditions of cultivation of the Liso-steppe and Polissya of Ukraine are highlighted. It was found that the core of the shoot, depending on the year of vegetation, and the buds were damaged the most by low temperatures on average during three years of research. The most resistant to low temperatures were the tissues of the bark and cambium, of the varieties — plants The highlight of the garden. This variety can be used for breeding work for winter hardiness to obtain stable forms.

Innovation Project: Cryomutation Selection of Sunflower for Winter Resistance (wint), Ephemeral State (pervotsvet) and Leaflessness (aphylla)

Helia ◽  
2016 ◽  
Vol 39 (65) ◽  
pp. 213-224
Author(s):  
Ashot Andranikovich Kalaidzhyan
Keyword(s):  
Low Temperatures ◽  
Dimethyl Sulfate ◽  
Growth Period ◽  
Induced Mutations ◽  
Petiole Length ◽  
Stem Form ◽  
Innovation Project ◽  
Shape Changes ◽  
Selection Of

AbstractBased on long-term work on induced mutations using dimethyl sulfate (DMS) at medium doses (concentrations) a large number of mutagenic lines was obtained. The source cultivar for obtaining the mutants was VNIIMK 8931. The mutations were obtained by changing the growth period, plant height, stem form, changes of the head (size, form, and shape), changes of the leaves (color, size, form, serratedness, petiole length), and changes of the size, shape, color and levels of oil and protein and their quality. The most important mutations presented in this paper pertain to resistance to low temperatures (up to –30 °C) in what can be termed winter sunflower (M-2002-Morzh and M-1701-Ice King). Of great value is also the mutant M-1880-Udochka (aphylla leafless) with its small leaves and short petioles, which enables the increase in plant population of up to 500,000 plants/ha.

scholarly journals Traditional and Modern Protective Media for the Low-Temperature Bacteria Preservation

2011 ◽  
pp. 36-40
Author(s):  
I. V. Gracheva ◽  
T. V. Valova ◽  
G. V. Grigor'eva
Keyword(s):  
Low Temperature ◽  
Dimethyl Sulfoxide ◽  
Low Temperatures ◽  
Pathogenic Bacteria ◽  
Effective Application ◽  
Regulatory Guidelines ◽  
Protein Origin

Presented are the literature data on the efficiency of bacteria preservation at temperatures ranging from -20 to -196 °C in the protective media containing such cryoprotectors as glycerol, dimethyl sulfoxide, carbo-hydrates, substances of protein origin specified by regulatory guidelines. Most of the focus is on the publications reporting the results of the long-term bacteria preservation at sub-zero temperatures, optimization of the protective media for pathogenic bacteria, and usage of the compounds with potential cryoprotective activity. Noted is the necessity for approbation of the specified protective media for the conserved bacteria species at the applied preserving temperatures. One of the approaches to the enhancement of the low-temperature preservation techniques is a search for natural protectors, which can provide for surviving of bacteria in the unfavorable conditions, including low temperatures, and a search for possibility to integrate these natural protectors into the cryoprotective media. Displayed are the results of effective application of glycerol-betaine, and polysaccharides of Arctic microorganisms for the low-temperature bacteria preservation.

scholarly journals Perlakuan Panas untuk Mengurangi Gejala Kerusakan Dingin pada Mangga (Mangifera indica L.) var. Gadung selama Penyimpanan pada Suhu Rendah

2017 ◽  
Vol 1 (1) ◽  
pp. 8
Author(s):  
Gysberth Pattiruhu ◽  
Yohannes Aris Purwanto ◽  
Emmy Darmawanty
Keyword(s):  
Low Temperature ◽  
Low Temperatures ◽  
Mangifera Indica ◽  
Chilling Injury ◽  
Hot Water ◽  
Low Temperature Storage ◽  
Stage Of Development ◽  
Chemical Content ◽  
Temperature Storage

Mango (Mangifera indica L.) is perishable horticulture product. Commercially, mango fruits are harvested at the mature-green stage of development and are handled at low temperatures to facilitate shipping. However, long term low temperature storage of mature-green mango fruits is currently risky because of chilling injury (CI). The ojective of this study was to examine quality changes of mango during storage at 8 oC after heatshock treatment. Heatshock treatment consist of hot water treatment (HWT) at temperature of 55 oC in 3 and 10 minutes and intermittent warming (IW) at temperature 20 oC for 1 day after 2 and 3 days of low temperature storage. The result showed that HWT of 55 oC in 3 minutes could decrease of weight loss and maintain the quality and chemical content of mango during storage at low temperatures. While IW treatment after 2 and 3 days at low temperature storage in this study was not effective in reducing chilling injury and maintaining quality of mango.Keywords: chilling injury, hetshock treatment,low temperature storage, mango

scholarly journals Stress and Strain Modeling of Low Temperature Cofired Ceramic (LTCC) Seal Frame and Lid

2015 ◽  
Vol 2015 (CICMT) ◽  
pp. 000157-000163
Author(s):  
Daniel Krueger ◽  
John Porter ◽  
Ken Peterson
Keyword(s):  
Low Temperature ◽  
High Reliability ◽  
High Density ◽  
Stress And Strain ◽  
Multichip Modules ◽  
Materials Used ◽  
Strain Modeling ◽  
Sealing Materials ◽  
Selection Of

Low temperature cofired ceramic (LTCC) is established as an excellent packaging technology for high reliability, high density microelectronics. LTCC multichip modules (MCMs) comprising both ‘surface mount’ and ‘chip and wire’ technologies provide additional customization for performance. Long term robustness of the packages is impacted by the selection of seal frame and lid materials used to enclose the components inside distinct rooms in LTCC MCMs. An LTCC seal frame and lid combination has been developed that is capable of meeting the sealing and electromagnetic shielding requirements of MCMs. This work analyzes the stress and strain performance of various seal frame and lid materials, sealing materials, and configurations. The application for the MCM will impact selection of the seal frame, lid, and sealing materials based on this analysis.

Induced bolting and anthesis in sugar beet and the effect of selection of physiological types

1946 ◽  
Vol 36 (3) ◽  
pp. 167-183 ◽  
Author(s):  
G. D. H. Bell
Keyword(s):  
Low Temperature ◽  
Sugar Beet ◽  
Low Temperatures ◽  
Continuous Light ◽  
Light Treatment ◽  
Seedling Stage ◽  
Temperature Exposure ◽  
Different Strains ◽  
Low Temperature Exposure ◽  
Selection Of

1. The effect of low-temperature and continuous-light treatment of seedlings of certain sugar beet strains in the isolation of distinct physiological types is described. The progenies of individual plants and bulk samples of different anthesis dates show some difference in the proportions of early and late plants, when these progenies are similarly exposed to low temperatures and continuous light in the seedling.2. Different treatments of progenies in relation to light treatment in the seedling stage result in distinct behaviour with regard to anthesis date and the number of plants reaching the stage of anthesis.3. The comparative resistance to bolting from a field sowing of seed obtained from plants which ripened seed after continuous light treatment of the seedlings is noted. This was so in spite of the fact that only 66% of the plants had reached anthesis as a result of the treatment, and the seed was obtained from the most rapidly bolting plants.4. Light and low temperature exposure of seedlings is effective in two different strains for isolating bolting-resistant types as measured directly from the seed of these bolting-resistant plants and selected progenies from these plants. Differences in the field germination and establishment from very early sowings were discernible in the progenies.

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