scholarly journals Phenolic profiles and antioxidant activities in selected drought-tolerant leafy vegetable amaranth

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Umakanta Sarker ◽  
Shinya Oba
Keyword(s):  
Antioxidant Activities ◽  
Pharmacological Study ◽  
Sinapic Acid ◽  
Syringic Acid ◽  
Leafy Vegetable ◽  
Coumaric Acid ◽  
Drought Tolerant ◽  
Phenolic Profiles ◽  
Phenolic And Flavonoid ◽  
Phenolic And Flavonoid Compounds

Abstract Four selected advance lines of drought-tolerant leafy vegetable amaranth were characterized for phenolic profiles, vitamins, and antioxidant activities. The selected advance lines exhibited differences in terms of genotypes with remarkable phenols, vitamins, flavonoids content, and potential radical quenching capacity. We identified twenty-five phenolic and flavonoid compounds including protocatechuic acid, salicylic acid, gentisic acid, gallic acid, β-resorcylic acid, vanillic acid, p-hydroxybenzoic acid, chlorogenic acid, ellagic acid, syringic acid, ferulic acid, kaempferol, m-coumaric acid, trans-cinnamic acid, quercetin, p-coumaric acid, apigenin, caffeic acid, rutin, sinapic acid, isoquercetin, naringenin, myricetin, catechin, and hyperoside. The selected advance lines VA14 and VA16 had abundant phenols, vitamins, flavonoids, and antioxidants potentiality. The selected drought-tolerant leafy vegetable amaranth showed high antioxidant potentiality as phenols, vitamins, flavonoids of these lines had a significant positive correlation with antioxidant capacities equivalent to Trolox using 2,2-diphenyl-1-picrylhydrazyl and ABTS+. Therefore, drought-tolerant leafy vegetable amaranth VA14 and VA16 can be grown in semi-arid and drought-prone areas in the world to attaining vitamins and antioxidant sufficiency. The phenolic and flavonoids compounds identified in drought-tolerant leafy vegetable amaranth demand a comprehensive pharmacological study. The baseline data on phenolic and flavonoids compounds obtained in the present study will contribute to the scientist forum for the scientific evaluation of these compounds in vegetable amaranth.

scholarly journals Polyphenol and flavonoid profiles and radical scavenging activity in leafy vegetable Amaranthus gangeticus

2020 ◽  
Vol 20 (1) ◽  
Author(s):  
Umakanta Sarker ◽  
Shinya Oba
Keyword(s):  
Phenolic Compounds ◽  
Phenolic Acids ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Sinapic Acid ◽  
Health Benefit ◽  
Leafy Vegetable ◽  
Scavenging Activity ◽  
Coumaric Acid ◽  
Phenolic Profiles

Abstract Background Red amaranth (Amaranthus gangeticus L.) has great diversity in Bangladesh, India, and South East Asia with multipurpose uses. The bright red-violet colored A. gangeticus is a popular and low-cost leafy vegetable in the Asian continent including Bangladesh and India because of attractive leaf color, taste, adequate nutraceuticals, phenolic compounds, and sole source of betalains. The natural colors and phenolic compounds of this species have a significant role in promoting the health-benefit including the scavenging capacity of radicals, the colorant of food products, and play a vital role in the industry of foods. However, phenolic profiles and radical scavenging activity of this species have not been evaluated. Hence, for the first time, four selected advance lines of A. gangeticus were characterized for phenolic profiles, antioxidant constituents, and antioxidant potentiality. Results A. gangeticus genotypes are abundant sources of phenolic profiles and antioxidant constituents with good radical quenching capacity that differed across the genotypes. Twenty-five phenolic acids and flavonoids, such as protocatechuic acid, salicylic acid, gentisic acid, gallic acid, β-resorcylic acid, vanillic acid, p-hydroxybenzoic acid, chlorogenic acid, ellagic acid, syringic acid, ferulic acid, kaempferol, m-coumaric acid, trans-cinnamic acid, quercetin, p-coumaric acid, apigenin, caffeic acid, rutin, sinapic acid, isoquercetin, naringenin, myricetin, catechin, and hyperoside were identified in A. gangeticus accessions. A. gangeticus accessions LS7 and LS9 demonstrated ample phenolic acids, flavonoids, antioxidant constituents, and antioxidant potentiality. It revealed from the correlation study that antioxidant components of A. gangeticus genotypes exhibited good radical scavenging activities. The genotypes LS7 and LS9 could be directly used as phenolic profiles, antioxidant constituents, and antioxidant activity enrich cultivars. Conclusions The identified compounds of phenolic acids and flavonoids in A. gangeticus privilege the comprehensive study of pharmacology. The basic information on phenolic profiles and antioxidant constituents achieved in the present study will provide the scientist’s forum for the scientific assessment of these compounds in A. gangeticus.

scholarly journals Polyphenolic Contents and Antioxidant Properties of Different Grape (V. vinifera,V. labrusca, andV. hybrid) Cultivars

2013 ◽  
Vol 2013 ◽  
pp. 1-5 ◽  
Author(s):  
Shivraj Hariram Nile ◽  
S. H. Kim ◽  
Eun Young Ko ◽  
Se Won Park
Keyword(s):  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Coumaric Acid ◽  
Grape Skin ◽  
Phenolic Contents ◽  
Grape Skins ◽  
Phenolic And Flavonoid

The polyphenolic contents and the antioxidant activity of the skins and pulps of different grape cultivars were estimated using HPLC and DPPH antioxidant assay, respectively. The phenolics and flavonoids identified were quercetin, kaempferol, caffeic acid, p-coumaric acid, cinnamic acid, and (−)-epicatechin. The total phenolic contents were found to be the highest in the grape skin of Flouxa (>400 mg/100 g), followed by Campbell Early and Tamnara (>300 mg/100 g), and then by Red Globe and Ruby Seedless (>250 mg/100 g), and the total phenolic content was the lowest in Italia and Delaware (<60 mg/100 g). The antioxidant activities of the grape extracts varied from 12.5% (Ruby Seedless) to 60.2% (Hongiseul) for skins, whereas the antioxidant activities of the grape extracts varied from 35.4% (Campbell Early) to 84.5% (Hongiseul) for pulps. The grape pulps have stronger antioxidant activities than those of the grape skins. Our results suggest that the phenolic and flavonoid contents in extracts of grape skins and pulps showed statistically significant correlations with the free radical scavenging activity.

Involvement of Phenolic Compounds in Anaerobic Flooding Germination of Rice (Oryza sativa L.)

2016 ◽  
Vol 56 ◽  
pp. 73-81 ◽  
Author(s):  
Do Tan Khang ◽  
Pham Thi Thu Ha ◽  
Nguyen Thi Lang ◽  
Phung Thi Tuyen ◽  
Luong The Minh ◽  
...  
Keyword(s):  
Phenolic Acids ◽  
Germination Rate ◽  
Syringic Acid ◽  
Seedling Vigor ◽  
High Concentration ◽  
Rice Varieties ◽  
Shoot Height ◽  
Phenolic Profiles ◽  
Total Difference ◽  
Phenolic And Flavonoid

By this study, thirty rice varieties were evaluated for anaerobic flooding tolerance using the direct sowing method. Phenolic profiles of strong and weak tolerant varieties were identified and compared based on HPLC chromatograms. The germination rates and shoot heights of rice were recorded for calculating the seedling vigor, which indicate the tolerant ability of rice in flooding condition. The results revealed a high variation of germination rate (10.01 to 100%), shoot height (0.35 to 78.17 mm) and seedling vigor (0.05 to 72.83). There was a high correlation between (r = 0.71) germination rate in 5 cm and 10 cm flood. Phenolic and flavonoid contents of the strong tolerant cultivar significantly and proportionally increased in the flooding levels (5 cm and 10 cm). There was a total difference in terms of number of phenolic acids found in the strong and weak tolerant varieties. In particular, six phenolic acids (gallic acid, catechol, caffeic acid, syringic acid, vanillin, and ellagic acid) were only identified with high concentration in the strong tolerant cultivar. The findings suggest that the phenolics presented in the strong tolerant varieties probably have a certain function in response and adaptation to anaerobic flooding condition. Further researches on exogenous application of these phenolic acids to increase the flooding tolerant level of rice should be continued at both green house and field treatments.

scholarly journals Total Flavonoid Content and Antioxidant Activity of Temu Mangga (Curcuma mangga Val. & Zijp) and its Classification with Chemometrics

2020 ◽  
pp. 4
Author(s):  
Yuniar Intan Hartono ◽  
Indah Widyastuti ◽  
Hanna Zaidah Luthfah ◽  
Rosy Islamadina ◽  
Adelin Theresia Can ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Methanolic Extract ◽  
Antioxidant Activities ◽  
Total Flavonoid ◽  
Total Flavonoid Content ◽  
Flavonoid Content ◽  
The Relationship ◽  
Phenolic And Flavonoid ◽  
Phenolic And Flavonoid Compounds

Indonesia is a rich country in terms of its biodiversity. Herbs that are widely used tomaintain a healthy body comes from the Zingiberaceae family, including temu mangga. Temu mangga(Curcuma mangga Val. & Zijp) is effective for relieving gastric pain, diarrhea, menstrual pain, acneand ulcers, and increasing appetite. Besides, temu mangga is considered to have antioxidant activitydue to the presence of the phenolic and flavonoid compounds. This study aims to determine thecorrelation and classification of temu mangga from various places based on total flavonoid contentand antioxidant activities. The samples were extracted with methanol, then tested for totalflavonoid content by AlCl3 method and antioxidant activity by DPPH free radical capture method.Analysis was performed using correlation coefficient value and chemometrics PCA (PrincipalComponent Analysis) and CA (Cluster Analysis) to see the relationship between total flavonoidcontent with their antioxidant activity and to see their classification. Total flavonoid content ofmethanolic extract of temu mangga gives a moderate correlation to its antioxidant activity with r =-0.6085 and clustering samples successfully determined by PCA and CA which resulted in 4 groups.Keywords: Curcuma mangga Val. & Zijp, Methanolic Extract, Flavonoid, Antioxidant, PCA, CA

scholarly journals Development of pasteurized coffee-flavored dairy beverage added with mint extract (Mentha x piperita)

2020 ◽  
Vol 42 ◽  
pp. e48474
Author(s):  
Bruno Fischer ◽  
Jean Carlo Rauschkolb ◽  
Rogério Luis Cansian ◽  
Ilizandra Aparecida Fernandes ◽  
Alexander Junges ◽  
...  
Keyword(s):  
Antioxidant Activities ◽  
Flavonoid Content ◽  
Color Intensity ◽  
Product Analysis ◽  
Sensory Acceptance ◽  
Mentha X Piperita ◽  
Physical Chemical ◽  
Development And Validation ◽  
Phenolic And Flavonoid ◽  
Phenolic And Flavonoid Compounds

The aim of this work was to evaluate different concentrations of ethanol on antioxidant (phenolic and flavonoid) compounds extraction of Mentha x Piperita aiming at applying the extract in whey-based dairy beverages. The extracts were obtained by cold maceration using water and ethanol as solvents. The extract using water and 70% ethanol had a 13.83 (w w-1) yield, 61.72 mg GAE g-1 phenolic compounds extract, 37.27 mg QE g-1 flavonoid content extract and 0.056 mg mL-1 antioxidant activities. The dairy beverage formulations followed a 22 factorial design in order to evaluate the effect of whey/milk and starch addition on the physical-chemical (pH, acidity, moisture, ash, fat, protein, viscosity, and color) and sensorial characteristics (acceptability) of the product. Analysis of variance (ANOVA) allowed for the development and validation of mathematical models for ash content, lipids, viscosity, and luminosity of the dairy beverage. The effects of whey/milk and starch variables on such characteristics were analyzed by the elaboration of contour curves. The product presented a lower color intensity at the highest whey concentration (75%). The product had a good sensory acceptance, and the 50 whey and 50% milk formulation presented a 7.16 points average and 79.51% acceptance index, suggesting that 50% of whey could be added up to the product’s formulation.

scholarly journals Antioxidant constituents of three selected red and green color Amaranthus leafy vegetable

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Umakanta Sarker ◽  
Shinya Oba
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Acids ◽  
Protocatechuic Acid ◽  
Pharmacological Study ◽  
Correlation Study ◽  
Syringic Acid ◽  
Coumaric Acid ◽  
Green Color ◽  
Red Color ◽  
Excellent Source

AbstractRed color (A. tricolor) genotypes are an excellent source of pigments, such as betalain (1122.47 ng g−1 FW), β-xanthin (585.22 ng g−1 FW), β-cyanin (624.75 ng g−1 FW), carotenoids (55.55 mg 100 g−1 FW), and antioxidant phytochemicals, such as vitamin C (122.43 mg 100 g−1 FW), TFC (312.64 RE µg g−1 DW), TPC (220.04 GAE µg g−1 DW), TAC (DPPH and ABTS+) (43.81 and 66.59 TEAC µg g−1 DW) compared to green color (A. lividus) genotype. Remarkable phenolic acids, such as salicylic acid, vanillic acid, protocatechuic acid, gallic acid, gentisic acid, β-resorcylic acid, p-hydroxybenzoic acid, syringic acid, ellagic acid, chlorogenic acid, sinapic acids, trans-cinnamic acid, m-coumaric acid, caffeic acid, p-coumaric acid, ferulic acid, and flavonoids, such as rutin, hyperoside, isoquercetin, myricetin, quercetin, apigenin, kaempferol, and catechin were observed in the red color amaranth genotypes, which was much higher compared to the green color amaranth genotype. We newly identified four flavonoids such as quercetin, catechin, myricetin, and apigenin in amaranth. Among the three selected advanced genotypes studied the red color genotype VA13 and VA3 had abundant antioxidant pigments, phytochemicals, phenolic acids, flavonoids, and antioxidant activity could be selected for extracting colorful juice. Correlation study revealed that all antioxidant constituents of red color amaranth had strong antioxidant activity. The present investigation revealed that two red color genotypes had an excellent source of antioxidants that demand detail pharmacological study.

scholarly journals Antifungal, Antibacterial, and Antioxidant Activities of Acacia Saligna (Labill.) H. L. Wendl. Flower Extract: HPLC Analysis of Phenolic and Flavonoid Compounds

Molecules ◽  
2019 ◽  
Vol 24 (4) ◽  
pp. 700 ◽  
Author(s):  
Asma Al-Huqail ◽  
Said Behiry ◽  
Mohamed Salem ◽  
Hayssam Ali ◽  
Manzer Siddiqui ◽  
...  
Keyword(s):  
Antioxidant Activities ◽  
Water Extract ◽  
Wood Products ◽  
Butylated Hydroxytoluene ◽  
Acacia Saligna ◽  
Flower Extract ◽  
Flavonoid Compounds ◽  
Carotovorum Subsp ◽  
Phenolic And Flavonoid ◽  
Phenolic And Flavonoid Compounds

In this study, for the environmental development, the antifungal, antibacterial, and antioxidant activities of a water extract of flowers from Acacia saligna (Labill.) H. L. Wendl. were evaluated. The extract concentrations were prepared by dissolving them in 10% DMSO. Wood samples of Melia azedarach were treated with water extract, and the antifungal activity was examined at concentrations of 0%, 1%, 2%, and 3% against three mold fungi; Fusarium culmorum MH352452, Rhizoctonia solani MH352450, and Penicillium chrysogenum MH352451 that cause root rot, cankers, and green fruit rot, respectively, isolated from infected Citrus sinensis L. Antibacterial evaluation of the extract was assayed against four phytopathogenic bacteria, including Agrobacterium tumefaciens, Enterobacter cloacae, Erwinia amylovora, and Pectobacterium carotovorum subsp. carotovorum, using the micro-dilution method to determine the minimum inhibitory concentrations (MICs). Further, the antioxidant capacity of the water extract was measured via 2,2′-diphenylpicrylhydrazyl (DPPH). Phenolic and flavonoid compounds in the water extract were analyzed using HPLC: benzoic acid, caffeine, and o-coumaric acid were the most abundant phenolic compounds; while the flavonoid compounds naringenin, quercetin, and kaempferol were identified compared with the standard flavonoid compounds. The antioxidant activity of the water extract in terms of IC50 was considered weak (463.71 μg/mL) compared to the standard used, butylated hydroxytoluene (BHT) (6.26 μg/mL). The MIC values were 200, 300, 300, and 100 µg/mL against the growth of A. tumefaciens, E. cloacae, E. amylovora, and P. carotovorum subsp. carotovorum, respectively, which were lower than the positive control used (Tobramycin 10 μg/disc). By increasing the extract concentration, the percentage inhibition of fungal mycelial was significantly increased compared to the control treatment, especially against P. chrysogenum, suggesting that the use of A. saligna flower extract as an environmentally friendly wood bio-preservative inhibited the growth of molds that cause discoloration of wood and wood products.

scholarly journals Screening of tarragon accessions based on physiological and phytochemical responses under water deficit

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Hasan Mumivand ◽  
Amin Ebrahimi ◽  
Alireza Shayganfar ◽  
Hamid Hassaneian Khoshro
Keyword(s):  
Water Deficit ◽  
Antioxidant Activities ◽  
Syringic Acid ◽  
Yield Potential ◽  
High Yield ◽  
Breeding Programs ◽  
Drought Tolerant ◽  
Relative Water ◽  
Coumarin Compounds ◽  
High Yield Potential

AbstractIn this study, screening of Artemisia dracunculus accessions was investigated under water deficit based on physiological and phytochemical traits. The results clearly indicated that water deficit significantly reduced the relative water content, chlorophyll, and carotenoid contents and increased malondialdehyde, electrolyte leakage, and antioxidant activities. The responses of tarragon accessions to water deficit, however, were inconsistent. The HPLC analysis revealed the presence of chlorogenic, syringic, ferulic, vanillic, chicoric, and p-coumaric acids as major phenolic acids, while quercetin and herniarin were detected as the predominant flavonoid and coumarin compounds in the extracts. Our findings revealed that the water deficit not only increased the amounts of herniarin, luteolin, apigenin, caffeic acid, and syringic acid, but also introduced quercetin that was not present under normal conditions in Estahbanat. Nevertheless, these results were highly impacted by the accession type. The results indicated that Hamadan, Varamin and Estahbanat accessions could be introduced as tolerant accessions. Given the very different responses of tarragon accessions to water deficit and the diversity between these accessions, the findings of the present study could be an effective step in identifying and achieving homogeneous, drought-tolerant and high-yield potential accessions, and may help tarragon breeding programs as well as development of cultivation.

scholarly journals Polyphenolic profiles in edible Annona spp. using high-performance liquid chromatography (HPLC-MS/MS)

2020 ◽  
Vol 21 (3) ◽  
pp. 201-208
Author(s):  
Manjunath J Shetty ◽  
K. R Vasudeva ◽  
T Sakthivel ◽  
G. J Suresh ◽  
H. C Krishna ◽  
...  
Keyword(s):  
High Performance ◽  
Biological Activities ◽  
Sinapic Acid ◽  
Syringic Acid ◽  
Polyphenolic Compounds ◽  
Hydroxybenzoic Acid ◽  
Coumaric Acid ◽  
Dihydroxybenzoic Acid ◽  
Normal Functioning ◽  
Polyphenolic Composition

Polyphenolic compounds of fruits of various plant species play an important role in physiological functions related to human health. Polyphenols have important biological activities like antioxidant activity which helps in normal functioning of human body. The objective of this study was to investigate the profiles of polyphenolic compounds in different edible Annona spp. fruits. Total of eighteen identified individual phenolic compounds were found, among which p-coumaric acid (321.53-90.17µg g-1FW), o-coumaric acid (70.80-19.00µg g-1FW), 2,4-dihydroxybenzoic acid (39.49-10.43µg g-1FW), caffeic acid (35.26-3.43µg g-1FW), gentisic acid (24.69-10.46µg g-1FW), protocatechuic acid (17.04-4.23µg g-1FW), t-cinnamic acid (22.68-3.93µg g-1FW) and ferulic acid (21.78-3.43µg g-1FW) were abundant in annona fruits while benzoic acid (23.28-4.61µg g-1FW), p-hydroxybenzoic acid (1.79-0.31µg g-1FW), salicylic acid (6.00-2.40µg g-1FW), 3-hydroxybenzoic acid (6.05-0.88µg g-1FW), vanillic acid.  (19.13-2.16µg g-1FW), gallic acid (15.88-2.74µg g-1FW), ellagic acid (1.12-0.20µg g-1FW), syringic acid (0.78-0.34µg g-1FW) and sinapic acid (2.16-0.79µg g-1FW) were limited. However, chlorogenic acid was not detected. The results obtained in this study will furnish a better knowledge of the polyphenolic composition in annona fruits.

Effect of gamma irradiation processing on total phenol and antioxidant capacities of the Iranian extract of propolis

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Marzieh Heidarieh ◽  
Ali Nabipour Chakoli ◽  
Samira Shahbazi ◽  
Parvin Shawrang ◽  
Baode Zhang
Keyword(s):  
Gamma Irradiation ◽  
Antioxidant Activities ◽  
Reducing Power ◽  
The Other ◽  
Total Phenolic ◽  
Phenolic Contents ◽  
Flavonoid Compounds ◽  
Gamma Irradiated ◽  
Phenolic And Flavonoid ◽  
Phenolic And Flavonoid Compounds

Abstract The Iranian ethalonic extract of propolis (EEP) were analyzed to determine their antioxidant activities and total phenolic and flavonoid contents, with and without gamma irradiation processing under various dosage of gamma (10, 20, 30, 40 and 50 kGy). The analysis results show that the gamma irradiated EEP at 10 KGy can scavenge free radicals and exhibit higher antioxidant-reducing power to the other groups (P < 0.05). It is noted that gamma irradiated EEP has higher total phenolic contents compared to gamma irradiated EEP at 30, 40 and 50 KGy and non-irradiated EEP (P < 0.05). The flavonoid compounds as indicated by C-13 NMR analysis were found to be higher in gamma irradiated EEP at 10 kGy compared with the other groups. In conclusion, gamma irradiation processing of EEP causes can enhanced antioxidant activities phenolic and flavonoid compounds at dose of 10 KGy.

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