scholarly journals Green tea consumption increases sperm concentration and viability in male rats and is safe for reproductive, liver and kidney health

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Chinyerum Opuwari ◽  
Thomas Monsees
Keyword(s):  
Green Tea ◽  
Seminiferous Tubule ◽  
Acrosome Reaction ◽  
Sperm Concentration ◽  
Sperm Parameters ◽  
Male Rats ◽  
Male Rat ◽  
Dependent Manner ◽  
Antioxidant Power ◽  
Liver And Kidney

Abstract Green tea is a popularly consumed beverage worldwide and contains polyphenols, whose antioxidant activities could improve sperm parameters and fertility thereof. We investigated the effect of green tea on the male rat reproductive system as well as its safety. Male Wistar rats were administered 2 and 5% aqueous extract of green tea for 52 days’ ad libitum, while the control group received tap water. Total polyphenol, flavanol, flavonol and soluble solids significantly increased in a concentration-dependent manner in vitro (P < 0.01). Weights of body, testis, epididymis, prostate gland, seminal vesicles, and liver, serum levels of testosterone, ferric reducing antioxidant power, creatinine, and sperm motility, remained unchanged (P > 0.05). Kidney weight, sperm concentration and vitality, spontaneous acrosome reaction increased (P < 0.05), while alanine transaminase and aspartate transaminase levels decreased (P < 0.05). Catalase, superoxide dismutase, glutathione and lipid peroxidation remained unchanged in the testes, liver and kidney (P > 0.05). Histological sections of testis, epididymis, kidney and liver showed no conspicuous alteration. Diameter and epithelial height of seminiferous tubule decreased, while caudal epididymis epithelial height increased (P < 0.01). Consumption of green tea in the conditions used in the present study seems to be safe and improved sperm parameters. However, subtle structural changes observed in the decreased diameter and epithelial height of the seminiferous tubule and increased acrosome reaction needs further investigation.

Evaluation of diuretic and antioxidant properties in aqueous bark and fruit extracts of pine

2020 ◽  
Vol 17 ◽  
Author(s):  
Hadi Shariati ◽  
Mohammad Hassanpour ◽  
Gholamreza Sharifzadeh ◽  
Asghar Zarban ◽  
Saeed Samarghandian ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Content ◽  
Antioxidant Properties ◽  
Pine Bark ◽  
Male Rats ◽  
Total Phenolic ◽  
Dependent Manner ◽  
Aqueous Extracts ◽  
Antioxidant Power ◽  
Dose Dependent

Objective: The present study has been carried out to evaluate the diuretic and antioxidant properties of pine herb in an animal model. Materials and Methods: 45 adult male rats were randomly divided into nine groups including: groups I (the negative control), groups II (positive control, furosemide 10 mg/kg), groups III to VIII (treatment groups received 100, 200, 400 mg/kg of the aqueous extracts of bark and fruit) and group IX received the combination of aqueous extract of bark (100 mg/kg) and the fruit (100 mg/kg). The urine output, glomerular filtration rate (GFR), electrolytes, urea, and creatinine levels were evaluated . Furthermore, the phenolic content and antioxidant activity of both extracts were also assessed using 2, 2-diphenyl-1-picrylhydrazyl (DPPH), ferric reducing antioxidant power (FRAP) and Folin–Ciocalteu methods. Results: The aqueous extracts of the pine bark and fruit increased the urinary output in a dose-dependent manner. The combination of the two extracts compared to the other extracts alone significantly increased the serum potassium level. This study also showed each extract increase creatinine clearance in a dose-dependent manner (p<0.01 and p<0.05). The increase of GFR in the combination group was not significant. The current data showed a significant increase in the total phenolic content in pine bark extract in compared with the fruit extract. Conclusion: The pine bark and fruit can be useful in the prevention and treatment of kidney stones due to the high antioxidant activity.

scholarly journals Curcumin Along With Fe3O4 Nanoparticles Improved Sperm Parameters In Rats With Testicular Ischemia

2021 ◽  
Vol 10 ◽  
pp. e2034
Author(s):  
Shabnam Zarei Moradi ◽  
Seyed Abdolhamid Angaji ◽  
Mitra Salehi ◽  
Mehrdad Hashemi
Keyword(s):  
Fe3o4 Nanoparticles ◽  
Sperm Concentration ◽  
Concomitant Administration ◽  
Testicular Tissue ◽  
Sperm Parameters ◽  
Male Rats ◽  
Semen Parameters ◽  
Control Group ◽  
Fe3o4 Nps ◽  
Testicular Ischemia

Background: Ischemic/reperfusion (I/R) in testicular tissue is one reason for the worldwide increase in male infertility. In the present study, we assessed the effects of curcumin and Fe3O4 nanoparticles (NPs) on sperm parameters in rats with I/R damage. Materials and Methods: Forty-eight adult male rats were divided into two groups (n=24 per group): control and torsion/detorsion. The control and torsion/detorsion groups were divided into four subgroups include sham, Fe3O4 NPs, curcumin, and Fe3O4 NPs+curcumin. After the rats were sacrificed, semen was collected from their epididymal tissues to assess sperm viability, motility, concentration, and morphology. Results: Curcumin significantly improved viability, motility, and normal sperm morphology in rats with I/R damage compared to the control group; however, it did not have a significant effect on sperm concentration (P<0.001). Fe3O4 NPs alone decreased all sperm parameters in the control and I/R rats (P<0.001). However, concomitant administration of Fe3O4 nanoparticles with curcumin significantly improved sperm parameters in rats with I/R damage (P<0.001). Conclusion: The increase in all semen parameters in the experimental groups with concomitant use of Fe3O4 NPs plus curcumin indicated that green synthesis of NPs could be recommended for future clinical studies.

Somatostatin Inhibits prolactin secretion in the estradiol primed male rat

1981 ◽  
Vol 59 (10) ◽  
pp. 1082-1088 ◽  
Author(s):  
G. R. Cooper ◽  
S. H. Shin
Keyword(s):  
Hormone Secretion ◽  
Growth Hormone Secretion ◽  
Blocking Agent ◽  
Prolactin Secretion ◽  
Male Rats ◽  
Male Rat ◽  
Dependent Manner ◽  
Intact Male ◽  
Plasma Prl

Somatostatin inhibits not only growth hormone secretion, but also the secretion of several other hormones. The role of somatostatin in prolactin (PRL) secretion has not been clearly demonstrated. The present study was undertaken to examine the effects of somatostatin on rat PRL secretion in several different circumstances where the circulating PRL level is elevated: (1) the estradiol primed intact male rat, (2) normal and (3) estradiol primed rats pretreated with pimozide, (4) normal and (5) estradiol primed hypophysectomized male rats with adenohypophyses grafted under the kidney capsule (HAG rat). Blood samples (70 μL) were taken every 2 min via an indwelling atrial cannula from conscious, unrestrained animals. In the estradiol primed intact rats, a bolus injection of somatostatin (10, 100, and 1000 μg/kg) lowered PRL levels in a dose-dependent manner. When the PRL concentration was elevated by the administration of pimozide (3 mg/kg), a dopaminergic receptor blocking agent, somatostatin was ineffective in decreasing plasma PRL concentration but the PRL concentration was lowered by somatostatin when the rat had been primed with estradiol. Somatostatin had no effect on the normal HAG rats, but lowered the plasma PRL concentration in the estradiol primed HAG rats. Since somatostatin inhibits PRL secretion only in the estradiol primed rats, it is suggested that estradiol priming creates a new environment, presumably via new or altered receptors, which can be inhibited by somatostatin.

scholarly journals Sex-, tissue-, and exposure duration-dependent effects of imidacloprid modulated by piperonyl butoxide and menadione in rats. Part I: oxidative and neurotoxic potentials

2014 ◽  
Vol 65 (4) ◽  
pp. 387-398 ◽  
Author(s):  
Mustafa Yardimci ◽  
Yusuf Sevgiler ◽  
Eyyup Rencuzogullari ◽  
Mehmet Arslan ◽  
Mehmet Buyukleyla ◽  
...  
Keyword(s):  
Cholinesterase Activity ◽  
Specific Effect ◽  
Piperonyl Butoxide ◽  
Female Rats ◽  
Male Rats ◽  
Male Rat ◽  
Total Protein Content ◽  
Sprague Dawley ◽  
Adverse Action ◽  
Liver And Kidney

Abstract Earlier research has evidenced the oxidative and neurotoxic potential of imidacloprid, a neonicotinoid insecticide, in different animal species. The primary aim of this study was to determine how metabolic modulators piperonyl butoxide and menadione affect imidacloprid’s adverse action in the liver and kidney of Sprague-Dawley rats of both sexes. The animals were exposed to imidacloprid alone (170 mg kg-1) or in combination with piperonyl butoxide (100 mg kg-1) or menadione (25 mg kg-1) for 12 and 24 h. Their liver and kidney homogenates were analysed spectrophotometrically for glutathione peroxidase, glutathione S-transferase, catalase, total cholinesterase specific activities, total glutathione, total protein content, and lipid peroxidation levels. Imidacloprid displayed its prooxidative and neurotoxic effects predominantly in the kidney of male rats after 24 h of exposure. Our findings suggest that the observed differences in prooxidative and neurotoxic potential of imidacloprid could be related to differences in its metabolism between the sexes. Co-exposure (90-min pre-treatment) with piperonyl butoxide or menadione revealed tissue-specific effect of imidacloprid on total cholinesterase activity. Increased cholinesterase activity in the kidney could be an adaptive response to imidacloprid-induced oxidative stress. In the male rat liver, co-exposure with piperonyl butoxide or menadione exacerbated imidacloprid toxicity. In female rats, imidacloprid+menadione co-exposure caused prooxidative effects, while no such effects were observed with imidacloprid alone or menadione alone. In conclusion, sex-, tissue-, and duration-specific effects of imidacloprid are remarkable points in its toxicity

Effect of herbal aphrodisiac, Dioscorea bulbifera on sperm parameters, serum hormonal levels and testicular histology of Wistar rats

2020 ◽  
Vol 06 ◽  
Author(s):  
Thomson Msiska ◽  
Anthony Mwakikunga ◽  
David Tembo ◽  
Fanuel Lampiao
Keyword(s):  
Body Weight ◽  
Secondary Metabolites ◽  
Wistar Rats ◽  
Health Benefits ◽  
Sperm Concentration ◽  
Sperm Parameters ◽  
Male Rat ◽  
The World ◽  
Dioscorea Bulbifera ◽  
Hormonal Levels

Aim: The present study was aimed at screening phytochemicals of Dioscorea bulbifera extracts and evaluating its effects on male rat reproductive system. Background: Since ancient times, man has found a lot of health benefits from medicinal plants. According to the World Health Organisation (WHO) about 80% of the world population depends on traditional medicine, which is 90% plant-based,for their health needs [1]. In respect of this, men in Africa use herbal medicines in the management of erectile dysfunction and infertility [2]. Amongst thousands of herbs, folklore has implicated Dioscorea bulbifera bulbils in the treatment of piles, dysentery, syphilis, ulcers, cough, leprosy, diabetes, asthma, cancer and infertility [3]. Despite such great health benefits from D. bulbifera, in vitro studies and clinical data in support of its efficacy as an aphrodisiac are scanty. Objective: The objective of the study was to investigate the effects of Dioscorea bulbifera extracts on Wistar rat sperm parameters, hormonal levels and histology of the testis. Method: Thirty healthy adult male Wistar rats were divided into 5 groups of 6 rats each and were given different treatments of Dioscorea bulbifera extracts by oral gavage at 200 and 400 mg kg-1 body weight. After 8 weeks, body and reproductive organ weights, sperm concentration, hormonal levels and histological structure of the testis were evaluated. A calorimetric method was used to assess the quantity of phytochemical constituents. Results: Testosterone, luteinizing hormone, estradiol and follicle stimulating hormone levels were significantly (p<0.05) enhanced whereas prolactin levels were significantly (p<0.05) down-regulated in the treated groups. Total sperm motility, sperm concentration and the percentage of progressive motile cells increased compared to the control. All the treated animals showed an increase in body weight, seminiferous tubule diameter and germinal epithelial height with the testes showing all the stages of spermatogenesis. Phytochemical screening of the methanol extracts revealed the presence of alkaloids, steroids, flavonoids, phenols and saponins. Conclusion: Dioscorea bulbifera has secondary metabolites that seem to stimulate androgenic and anabolic effects. These secondary metabolites appear to be responsible for the positive changes in testicular architecture, serum hormonal levels, caudal sperm concentration and motility parameters. This study supports the ethno-aphrodisiac claims that have been made against it.

scholarly journals Effects ofNigella sativa(Habbatus sauda) Oil and Nicotine Chronic Treatments on Sperm Parameters and Testis Histological Features of Rats

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Ng Cho Ping ◽  
Noor Hashida Hashim ◽  
Durriyyah Sharifah Hasan Adli
Keyword(s):  
Body Weight ◽  
Sperm Motility ◽  
Seminiferous Tubule ◽  
Corn Oil ◽  
Sperm Quality ◽  
Sperm Parameters ◽  
Male Rats ◽  
Seminiferous Tubules ◽  
Sprague Dawley ◽  
Histological Features

Twenty-fourSprague-Dawleymale rats (7–9 weeks old, 200–250 g) were divided into Nicotine (N) (0.5 mg/100 g body weight (BW), Nicotine Control (NC) (saline, 0.1 mL/100 g BW),Habbatus saudaoil (HS) (6.0 μL/100 g BW), andHabbatus saudaControl (HSC) (corn oil, 0.1 mL/100 g BW) groups and treated for 100 days. Sperm parameters and seminiferous tubules measurements were evaluated. The N showed a significantly lower sperm motility (1.03±0.05×106 sperm/mL) and percentage of normal (82.61±0.03%) and live (93.88±0.01%) sperm, higher value for the seminiferous tubule (253.36±1.83 μm) and lumen (100.15±2.38 μm) diameters and spermatogonia (19.85±0.39 μm) and spermatocytes (33.37±0.59 μm) layers, and thinner spermatid-sperm layer (22.14±0.71 μm) than the NC (P<0.05). The HS had significantly higher sperm motility (1.49±0.04×106 sperm/mL) and percentage of normal (90.61±0.01%) and live (96.98±0.01%) sperm, smaller lumen diameter (67.53±2.34 μm) and thinner spermatogonia (17.67±0.32 μm) and wider spermatid-sperm (36.95±0.79 μm) layers than the HSC (P<0.05). This research confirmed that nicotine reduced sperm motility and morphology of normal and live sperms and also affected the testis histology, whileHabbatus saudaoil increased sperm quality and gave better testis histological features.

scholarly journals Protective Role of Rockect Seed (Eruca sativa) Extract against Monosodium Glutamate-induced Hepato-renal Toxicity in Male Rats

2020 ◽  
pp. 1-10
Author(s):  
Ehab Tousson ◽  
Afaf El-Atrash ◽  
Yosra Karson
Keyword(s):  
Renal Damage ◽  
Renal Toxicity ◽  
Monosodium Glutamate ◽  
Chloride Ions ◽  
Male Rats ◽  
Male Rat ◽  
Albino Rats ◽  
Male Adult ◽  
Liver And Kidney

Background and Objective: Monosodium glutamate (MSG) is identified as an Accent that is used in the food industry as a flavour enhancer with an umami taste that intensifies the meaty, savoury flavour of food. The present study aimed at evaluating the protective and ameliorative role of rocket seeds extract against monosodium glutamate-induced hepatic renal toxicity and oxidative stress in the male rat. Materials and Methods: A total of 60 male adult albino rats were equally divided into six groups (G1, Control; G2, rocket seeds (RS); G3, ACCENT or MSG; G4, Co- treated (RS+MSG); G5, Post- treated (MSG+RS); G6, Self-treated MSG).  Results: Current results revealed that; a significant increase in serum ALT, AST, ALP, AFP, Urea, Creatinine, potassium ions, chloride ions, cholesterol, triglyceride, HDL, and LDL levels in MSG as compared to control and RS groups. In contrast; a significant decrease in serum albumin, total proteins, catalase, GSH and SOD in liver and kidney homogenates in MSG as compared to control and RS groups. Co- or post-treatment of MSG with rocket seeds improved this change in liver and kidney functions, with best results for co-treatment than post and self-treatment. Conclusion: These findings suggested that the misuse of monosodium glutamate may contribute to continuous hepatic and renal damage. This shows that the desired dose of monosodium glutamate can safely be used with grapes seed in improving hepatic and renal damage in monosodium glutamate in young rats.

Subacute co-exposure to low doses of ruthenium(III) changes the distribution, excretion and biological effects of silver ions in rats

2020 ◽  
Vol 17 (2) ◽  
pp. 163 ◽  
Author(s):  
Nicoleta Vedeanu ◽  
Cezara Voica ◽  
Dana Alina Magdas ◽  
Bela Kiss ◽  
Maria-Georgia Stefan ◽  
...  
Keyword(s):  
Biological Effects ◽  
Female Rats ◽  
Male Rats ◽  
Male Rat ◽  
Human Organism ◽  
Critical Element ◽  
Female Rat ◽  
Low Doses ◽  
Subacute Toxicity ◽  
Liver And Kidney

Environmental contextAlthough ruthenium is a technology-critical element used in several new industries, little is known about its environmental impact or possible human health risks. We report a toxicological evaluation of laboratory animals during co-exposure to ruthenium and silver. We identified interactions between the two elements that could lead to unwanted biological effects. AbstractRuthenium is one of the rarest metals on Earth that is classified as a technology-critical element (TCE). Silver, however, is well known for its antibacterial and immunostimulant properties. The increasing use of Ru and Ag in medicine and daily life makes simultaneous exposure possible, with unknown pharmacokinetic or toxicological consequences for the human organism. Thus, the present study investigated the influence of co-exposure to RuIII on the subacute toxicity of Ag ions in rats after repeated daily administration for 28 days of low doses by oral gavage. The subacute toxicity was investigated by the evaluation of several biochemical and hematological parameters, evaluation of specific oxidative stress biomarkers from liver and kidney, and histopathological investigation of liver and kidney tissue samples after 28 days of exposure in female rats. In addition, Ag and Ru concentrations were determined by inductively coupled plasma mass spectrometry (ICP-MS) in urine, liver and kidney parenchyma in male rats. The obtained results showed that co-exposure to low doses of RuIII and Ag did not produce any significant biochemical, hematological or histopathological alterations in the treated female rat groups, except for an increased red cell distribution width (RDW) value. A decrease of urinary excretion of Ag and of the Ag concentration in kidneys was observed in the male rat group co-exposed to RuIII and Ag. This is the first invivo study investigating the toxic effect of co-exposure to low doses of Ag and Ru ions, and the obtained results may justify further research on this subject, mainly on the investigation of possible competitive mechanisms.

scholarly journals Increased Productivity towards the Success of Nothopanax Scutellarium in Case Of Hepatoprotector and Nefroprotector

2021 ◽  
Vol 4 (2) ◽  
pp. 1832-1837
Author(s):  
Sri Wahyuni Nasution
Keyword(s):  
Metabolic Disorders ◽  
Clinical Problem ◽  
Normal Activity ◽  
The Body ◽  
Male Rats ◽  
Male Rat ◽  
Adaptation Period ◽  
Treatment Groups ◽  
Impaired Liver ◽  
Liver And Kidney

This study aims to determine the concentration of bowl leaf extract (Nothopanax scutellarium) which is effective in protecting impaired liver and kidney function. Liver damage can cause metabolic disorders in the body, which will lead to homeostatic disorders. Acute renal failure is a clinical problem, occurring frequently and characterized by a relatively sudden decline in renal function. This research is qualitative experimental research using a male rat (Rattus norvegicus) which is healthy and has normal activity, with a bodyweight of 200 g. Male rats were adapted to be kept in cages for 7 days. Adaptation needed to be done to avoid the risk of stress in mice which could affect blood serum content. During the adaptation period, male rats are only given standard food and have not been given any treatment. At the end of the adaptation period, serum analysis was carried out in the five treatment groups. The results of the data obtained from this study were analyzed using the Oneway-Anova test.

scholarly journals Effects of phosalone plant pesticide on sperm parameters and sexual hormone levels in Wistar rats: An experimental study

2020 ◽  
Author(s):  
Fahime Mazaheri ◽  
Kazem Kamali Aliabad ◽  
Seyed Mehdi Kalantar ◽  
Noosha Ziya ◽  
Arezoo Khoradmehr ◽  
...  
Keyword(s):  
Experimental Study ◽  
Sex Hormones ◽  
Sperm Motility ◽  
Enzyme Inhibitor ◽  
Food Group ◽  
Sperm Parameters ◽  
Male Rats ◽  
Dependent Manner ◽  
Organophosphate Insecticide ◽  
Negative Effects

Background: Phosalone is an organophosphate insecticide, applied to control of plant pests. This compound has various side effects because it acts as an acetyl cholinesterase enzyme inhibitor. Objective: To investigate the effects of phosalone on the sperm parameters of and levels of sex hormones in adult male rats. Materials and Methods: In this experimental study, 16 adult (8-12 wk) male Wister rates (weighing 220-280 gr) were randomly assigned into 4 groups (n = 4/each). Group 1 (control) received only routine adequate water and food; Group 2, 3, and 4 received different low doses of phosalone (60, 90, and 120 mg/kg respectively). The rats were weighed and anesthetized after 48 days. Sperm parameters including number, motility, and viability as well as sex hormones (such as Luteinizing Hormone, Follicle Stimulating Hormone, and testosterone) were evaluated and compared after removing the epididymis tail. Results: Our results showed that phosalone decreased sperm motility, viability, and number in a dose-dependent manner. The level of FSH and LH was increased, and testosterone was decreased. Also, depending on the dose, phosalone decrease sperm motility and viability (p ≤ 0.001), while the level of FSH and LH was increased and testosterone was decreased (p = 0.861). Conclusion: Phosalone has negative effects on reproductive indices in male rats and can cause serious damage and decrease the number and sperms motility. It can also cause infertility due to changing the concentration of hormones. Key words: Organophosphate, Pesticides, Phosalone, Sperm, Sex hormones.

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