scholarly journals Impact of concurrent diabetes on periodontal health in patients with acromegaly

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Akanksha Jain ◽  
Shipra Gupta ◽  
Anil Bhansali ◽  
Mili Gupta ◽  
Ashish Jain ◽  
...  
Keyword(s):  
Pituitary Hormones ◽  
Healthy Controls ◽  
Periodontal Health ◽  
Blood Samples ◽  
Periodontal Status ◽  
Significant Difference ◽  
Treatment Naïve ◽  
Active Acromegaly ◽  
Serum Gh ◽  
Tgf Β1

Abstract Previous studies have suggested excess GH/IGF1 secretion in patients with acromegaly is protective for periodontal health. Diabetes is prevalent comorbidity in patients of acromegaly and is associated with worsening of periodontal disease. The present study evaluates the periodontal health and cytokines status in treatment-naive active acromegaly patients with and without diabetes. Eleven patients, each of acromegaly with and without diabetes and 20 healthy controls were enrolled. Periodontal parameters were assessed. GCF and blood samples for IL-6, TGF-β1, and PDGF were obtained. Serum GH, IGF1, HbA1c, pituitary hormones and MRI sella were performed in patients with acromegaly. There was no significant difference in periodontal status of patients with acromegaly and healthy controls. However, a significant increase in serum IL-6 (p = 0.019) and TGF-β1 (p = 0.025) levels in patients with acromegaly was observed and all patients had concurrent hypogonadism. Nevertheless, the patients with acromegaly having diabetes had modestly higher CAL and PD and serum IL-6 levels (p = 0.051), but it could not exert adverse effects on periodontal health in presence of GH/IGF1 excess. GH/IGF1 excess did not exert a protective effect on periodontal status in acromegaly, possibly due to concurrent hypogonadism and opposing cytokines; however, it could mask the ill-effects of diabetes on periodontal health.

scholarly journals Serum Cytokine Levels in Term and Preterm Deliveries Relating to the Periodontal Health of Mothers: A Pilot Study

2015 ◽  
Vol 4 (2) ◽  
pp. 109-115
Author(s):  
Istvan Gorzo ◽  
Tibor Novák ◽  
Hajnalka Orvos ◽  
Mariann Kovács ◽  
Barbara Bóka ◽  
...  
Keyword(s):  
Birth Weight ◽  
Preterm Birth ◽  
Pilot Study ◽  
Serum Cytokine ◽  
Periodontal Health ◽  
Term Birth ◽  
Periodontal Status ◽  
Tnf Α ◽  
Significant Difference ◽  
Cytokine Levels

ABSTRACT Background The aim of the study was to evaluate serumlevels of interleukin-1, beta (IL-1β) and tumor necrosis factoralpha (TNF-α) at birth and compare the values in case of preterm birth and normal birth groups of mothers considering the mothers’ periodontal status. Materials and methods Blood samples from 81 women (preterm birth, 41 women, and term birth, 40 women) were collected within half an hour of after delivery. Serum levels of IL-1β and TNF-α were measured. Periodontal status was characterized by bleeding on probing (BOP) and probing depth (PD). Results The frequency of BOP differed significantly between preterm and term groups; however, mean PD did not show a significant difference. Serum IL-1β levels were significantly higher in the preterm birth group. The levels TNF-α were slightly bigger in the term birth group, the difference was significant. The rank correlation showed a significant negative relationship between serum IL-1β and TNF-α level and birth weight and the length of pregnancy, and also between BOP frequency and the length of pregnancy. Conclusion Within the limitations of the study, it was found that IL-1β and TNF-α levels were higher when the delivery occurred preterm and the birth weight was smaller; however, a significant increase of cytokines in the serum in connection with maternal periodontal disease was not detected. Periodontics of mothers was not associated with preterm birth in the sample. How to cite this article Radnai M, Novák T, Orvos H, Kovács M, Bóka B, Kele B, Gorzó I. Serum Cytokine Levels in Term and Preterm Deliveries Relating to the Periodontal Health of Mothers: A Pilot Study. Int J Experiment Dent Sci 2015;4(2):109-115.

scholarly journals Impact of immune checkpoint molecules on FoxP3+ Treg cells and related cytokines in patients with acute and chronic brucellosis

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Hua-Li Sun ◽  
Xiu-Fang Du ◽  
Yun-Xia Tang ◽  
Guo-Qiang Li ◽  
Si-Yuan Yang ◽  
...  
Keyword(s):  
T Cells ◽  
Immune Checkpoint ◽  
Treg Cells ◽  
Shanxi Province ◽  
Healthy Controls ◽  
Chronic Patients ◽  
Immune Checkpoint Molecules ◽  
Significant Difference ◽  
Acute Patients ◽  
Tgf Β1

Abstract Background The immunoregulatory functions of regulatory T cells (Tregs) in the development and progression of some chronic infectious diseases are mediated by immune checkpoint molecules and immunosuppressive cytokines. However, little is known about the immunosuppressive functions of Tregs in human brucellosis, which is a major burden in low-income countries. In this study, expressions of immune checkpoint molecules and Treg-related cytokines in patients with acute and chronic Brucella infection were evaluated to explore their impact at different stages of infection. Methods Forty patients with acute brucellosis and 19 patients with chronic brucellosis admitted to the Third People’s Hospital of Linfen in Shanxi Province between August 2016 and November 2017 were enrolled. Serum and peripheral blood mononuclear cells were isolated from patients before antibiotic treatment and from 30 healthy subjects. The frequency of Tregs (CD4+ CD25+ FoxP3+ T cells) and expression of CTLA-4, GITR, and PD-1 on Treg cells were detected by flow cytometry. Levels of Treg-related cytokines, including IL-35, TGF-β1, and IL-10, were measured by customised multiplex cytokine assays using the Luminex platform. Results The frequency of Tregs was higher in chronic patients than in healthy controls (P = 0.026) and acute patients (P = 0.042); The frequency of CTLA-4+ Tregs in chronic patients was significantly higher than that in healthy controls (P = 0.011). The frequencies of GITR+ and PD-1+ Tregs were significantly higher in acute and chronic patients than in healthy controls (P < 0.05), with no significant difference between the acute and chronic groups (all P > 0.05). Serum TGF-β1 levels were higher in chronic patients (P = 0.029) and serum IL-10 levels were higher in acute patients (P = 0.033) than in healthy controls. We detected weak correlations between serum TGF-β1 levels and the frequencies of Tregs (R = 0.309, P = 0.031) and CTLA-4+ Tregs (R = 0.302, P = 0.035). Conclusions Treg cell immunity is involved in the chronicity of Brucella infection and indicates the implication of Tregs in the prognosis of brucellosis. CTLA-4 and TGF-β1 may contribute to Tregs-mediated immunosuppression in the chronic infection stage of a Brucella infection.

Periodontal Status and Quality of Life in Adult Patients Attending a Tertiary Care Centre in Kathmandu Valley

2019 ◽  
Vol 3 (2) ◽  
pp. 70-74
Author(s):  
Sonika Shakya ◽  
Priti Shrestha ◽  
Sabina Poudel
Keyword(s):  
Quality Of Life ◽  
Oral Health ◽  
Tertiary Care ◽  
Research Report ◽  
Periodontal Health ◽  
Periodontal Status ◽  
Significant Difference ◽  
Bad Breath ◽  
The Impact

Introduction: Symptoms of periodontal disease like redness, bleeding on brushing, loosening of affected teeth, and persistent bad breath are not usually documented in a research report. Such symptoms are highly relevant from the patient’s point of view and often have a considerable adverse impact on their daily quality of life. Objective: The objective of this study was to assess the periodontal health status and its impact on quality of life. Methods: Clinical attachment loss of total 100 participants were measured at six sites of all teeth and patients divided into severity groups according to loss of attachment. The Nepalese version of the Oral Health Impact Profile (OHIP-14) was used to assess impact of periodontal status on patient’s quality of life. In addition, participants were also asked to complete a simple ‘yes/no’ checklist of symptoms relating to their periodontal health in the past year which included swollen gums, sore gums, receding gums, loose teeth, drifting teeth, bad breath, or toothache. Results: Overall OHIP-14 score significantly differed between patient groups. The impact of oral health on quality of life was greater in patients with high/severe periodontitis and the result was statistically significant (p=0.001). Conclusion: There is significant difference between oral health related quality of life in healthy and periodontally involved patients as assessed by using OHIP-14. Treatment strategies should focus on improving the quality of life of periodontal patients.

Periodontal Health Status and its Impact on the Quality of Life among Diabetics attending Medical and Dental Out Patient Departments of a Tertiary Care Centre of Nepal

2021 ◽  
Vol 5 (1) ◽  
pp. 3-9
Author(s):  
T. Bhagat ◽  
A. Shrestha ◽  
J. Rimal ◽  
R. Maskey ◽  
S.K. Agrawal ◽  
...  
Keyword(s):  
Quality Of Life ◽  
Oral Health ◽  
World Health ◽  
Diabetic Patients ◽  
Periodontal Health ◽  
Periodontal Status ◽  
Periodontal Index ◽  
Significant Difference ◽  
The Impact

Background: Diabetics are more prone to periodontal diseases leading to poor oral function affecting their quality of life. The objective of the study was to assess the impact of periodontal health on the quality of life among diabetics using the short version of the Oral Health Impact Profile (OHIP -14). Methods: It was a descriptive cross-sectional study. Data was collected using translated and validated Nepalese version of OHIP-14 questionnaire and clinical examination for periodontal status (Community Periodontal Index and Loss of Attachment index) was done using mouth mirror and World Health Organization probe under natural light. Data was entered and analyzed using SPSS version 11.5. Mann- Whitney U test and Kruskal-Wallis tests were used to compare OHIP-14 scores between genders and periodontal status respectively. Statistical significance was established at p<0.05. Results: One hundred and forty-five subjects with confirmed diabetes participated in the study. Overall, 41% were male participants were as 59% were females. Majority of the participants had calculus with CPI score 1 (n=131, 90.3%) and another majority had loss of attachment 3-5 mm with LOA score 1 (n=55, 37.9%). There was no significant difference in mean scores between two genders (p=0.231). The OHIP scores among participants with highest CPI and LOA scores had statistically significant difference (p=0.011 and p=0.006 respectively). Conclusions: Periodontal status was poor among diabetics with significant impact on their oral health related quality of life. Glycemic control along with periodontal maintenance is required to enhance quality of life among such patients. This might be possible with comprehensive medical approach for diabetic patients.

scholarly journals Salivary citrullinated proteins in rheumatoid arthritis and associated periodontal disease

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Ildikó Tar ◽  
Éva Csősz ◽  
Edit Végh ◽  
Karin Lundberg ◽  
Nastya Kharlamova ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Periodontal Disease ◽  
Control Group ◽  
Healthy Controls ◽  
Periodontal Status ◽  
Protein Levels ◽  
Reduced Height ◽  
Significant Difference ◽  
Citrullinated Proteins ◽  
Citrullinated Protein

AbstractPeriodontal disease (PD) can be an important precipitating factor in the production of citrullinated proteins. Its importance is emphasized, but it is not the only way to produce citrullinated proteins. The aim of the current study was to determine the periodontal conditions and the salivary citrullinated protein content in patients with rheumatoid arthritis (RA) compared to healthy controls. We also wished to correlate citrullinated protein levels in the saliva and serum biomarkers with the periodontal status and temporomandibular joint (TMJ) involvement of patients with RA. Twenty-three patients with RA and 17 healthy controls participated the study. Saliva samples were taken: citrulline content of saliva was measured. Blood test results for patients with RA were collected. TMJ disorders were described. Cariological and periodontal indices were registered. Periodontal conditions and periodontal staging were also registered. Comparison of measured values between groups was performed. Intragroup correlation of patients’ values was counted. The prevalence of TMJ complaints was significantly higher in the RA group (8/23) versus controls (1/17). The patients with RA had worse periodontal condition because more patients with RA had gingivitis with a significantly higher bleeding on probing (BOP) (RA: 22.4 ± 25.0%; controls: 6.36 ± 11.6%; p = 0.018). Gingival index (GI) was also significantly higher in the patients than in controls (RA: 0.68 ± 0.58; controls: 0.19 ± 0.38; p = 0.010). The citrullinated protein (relative) content of saliva did not differ significantly (p = 0.147) between patients with RA (1102.2 ± 530.8) and healthy controls (1873.1 ± 1594.9). In RA, the salivary anti-CCP levels positively correlated with PD staging (R = 0.464, p = 0.039) . Control subjects more commonly had healthy gingiva than RA patients. Moreover, in the control group more individuals had intact and reduced height periodontium than periodontitis compared to the RA group. There was no significant difference in the levels of salivary citrulline between patients with RA and controls, despite the significant differences in their periodontal status. Thus, salivary citrulline levels are not associated with RA disease severity.

scholarly journals Left Ventricular Assist Device (LVAD) Implantation Transiently Increases Plasma TGF-β1 in Heart Failure Patients: Potential Impact of Lvad on Platelet Activation

Blood ◽  
2019 ◽  
Vol 134 (Supplement_1) ◽  
pp. 3616-3616
Author(s):  
Hendra Setiadi ◽  
Ahmed El-Banayosy ◽  
Erin Krueger ◽  
Karim Kouzbari ◽  
Gostynska Sandra ◽  
...  
Keyword(s):  
Platelet Activation ◽  
Left Ventricular ◽  
Healthy Controls ◽  
Total Plasma ◽  
Ventricular Assist ◽  
Blood Samples ◽  
Cabg Surgery ◽  
Left Ventricular Assist ◽  
Tgf Β1 ◽  
Ecmo Therapy

Although survival and quality of life have improved in patients with advanced heart failure (HF) after implantation of left ventricular assist devices (LVADs), they still pose risks of hemocompatibility-related complications, including thrombosis and bleeding. Development of biomarkers predictive of these LVAD-associated complications could guide decision making for both clinicians and patients. Recently, we showed higher plasma TGF-β1 levels within one-week after implantation with a miniaturized mechanical-bearing axial-flow pump HeartMate II (HM-II), and reasoned that platelet activation by the rotor may have caused the release of TGF-β1 in plasma in HF patients (Mancini et al. Transl. Res. 2018; 192:15-29). Recent clinical trials with the newest LVAD, the Heartmate 3 (HM-3), which uses a fully magnetically-levitated pump, showed superior clinical outcomes, including significantly reduced incidences of pump thrombosis and stroke (Mehra et al. N Engl J Med. 2019; 380:1618-1627). In this study, we evaluated release of TGF-β1 in plasma following implantation of HM-II and HM-3 LVADs compared to either coronary artery bypass graft (CABG) surgery or extracorporeal membrane oxygenation (ECMO) therapy. We measured serial total TGF-β1 levels in 38 Stage-D HF patients (11 received HM-II and 27 received HM-3). As a control, we collected blood samples from 10 patients undergoing CABG surgery, and 10 patients receiving ECMO therapy following acute onset cardio-pulmonary failure. Blood samples were collected before and 4-8 hours after procedures, and thereafter daily for up to one week. Plasma was prepared by centrifuging blood at 12,000 rpm for 5 min at 4°C within 10 min of blood drawing, which reduces in vitro release of TGF-β1 from platelets and thus allows accurate measurement of plasma TGF-β1. Total TGF-β1 levels were measured after acidification and neutralization of samples using DUO-ELISA kit (R&D Systems). Baseline total plasma TGF-β1 levels were higher in HF patients before LVAD implantation than in healthy controls [4.7 ± 1.9 ng/mL in HF patients (n= 38); 3.3 ± 0.8 ng/mL in healthy controls (n= 6); p=0.006)]. Total TGF-β1 levels surged transiently to 14.6 ± 6.1 ng/mL within 4-8 hours after LVAD implantation [(p&lt;0.0001 compared to patients 4-12 hours after CABG surgery (3.6 ± 1.4 ng/mL) or ECMO therapy (4.9 ± 1.3 ng/mL)]. Interestingly, however, we found that the transient surge of TGF-β1 in HM-3 recipients was significantly lower than in HM-II recipients (Figure-1; p=0.04). TGF-β1 levels then gradually decreased and reached near basal levels 2-3 days after LVAD implantation, but remained significantly elevated in plasma of HM-II recipients until day 5 (p=0.049). TGF-β1 levels remained unchanged in both CABG and ECMO patients at all time points (Figure 1). We conclude that LVAD implantation causes a transient surge in total plasma TGF-β1 within a few hours after the procedure, presumably due to platelet activation by LVAD, not the surgery itself, as CABG or circulating blood through ECMO did not cause the surge. The observation that a reduced initial surge and lower levels of TGF-β1 in HM-3 vs. HM-II recipients needs further investigation to determine whether these differences are due to LVAD-specific factors (different rotors causing variable shear effects) or to confounding differences in implantation procedures, such as, by-pass time, cardiac tissue injury, number of platelet transfusions, blood suction with catheters etc. or other unknown factors. Our data suggest that serial TGF-β1 measurements after LVAD implantation may serve as a surrogate biomarker for platelet activation in association with hemocompatibility-related adverse events (Uriel et al. Circ. 2017; 135:2003-2012). Disclosures No relevant conflicts of interest to declare.

MicroRNA Expression Levels in Patients with Hashimoto Thyroiditis: A Single Centre Study

2020 ◽  
Vol 20 ◽  
Author(s):  
Hale Onder Yilmaz ◽  
Alper Han Cebi ◽  
Mustafa Kocak ◽  
Halil Onder Ersoz ◽  
Mevlit Ikbal
Keyword(s):  
Hashimoto Thyroiditis ◽  
Healthy Controls ◽  
Blood Samples ◽  
Expression Levels ◽  
Diagnosis Group ◽  
Significant Difference ◽  
Single Centre Study ◽  
Polymerase Chain ◽  
Moderate Positive Correlation

Objective: To determine the circulatory miRNA expression levels in patients with Hashimoto thyroiditis (HT) at the time of diagnosis and follow-up period compared with healthy controls. Methods: We collected blood samples from 34 patients with HT (4 males and 30 females) at the time of first diagnosis (Group P) and euthyroid period (Group E). Thirty-three healthy controls (Group H) blood samples were also included in the study. Expression levels of five different circulating miRNAs (miR-22, miR-141, miR-155, miR-375, miR-451) were evaluated using real-time polymerase chain reaction. Results: There was a significant difference in miR-375 levels between the P group and the H. Also, for miR-451, there was a significant difference between the P and E groups. Finally, there was a moderate positive correlation between thyroidstimulating hormone values and miR-22 expression levels for the P group. Conclusion: miRNAs have important roles at all stages of the diseases. More studies must be performed in all thyroid diseases and autoimmune diseases, including HT.

scholarly journals Dysregulation of lncRNAs in circulation of patients with periodontitis: results of a pilot study

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Soudeh Ghafouri-Fard ◽  
Leila Gholami ◽  
Elham Badrlou ◽  
Saba Sadeghpour ◽  
Naghme Nazer ◽  
...  
Keyword(s):  
Case Control Study ◽  
Inflammatory Disorder ◽  
Healthy Controls ◽  
Tissue Samples ◽  
Blood Samples ◽  
Chronic Inflammatory Disorder ◽  
Real Time Quantitative Pcr ◽  
Significant Difference ◽  
Non Coding Rnas ◽  
Control Study

Abstract Background Periodontitis is a chronic inflammatory disorder with a complex etiology. Long non-coding RNAs (lncRNAs) have been shown to affect pathoetiology of periodontitis. We aimed at identification of expression of five lncRNAs, namely Linc0116, Linc00667, CDK6-AS1, FENDRR and DIRC3 in the circulation and gingival tissues of these patients compared with healthy controls. Methods In a pilot case–control study, we compared expressions of Linc0116, Linc00667, CDK6-AS1, FENDRR and DIRC3 lncRNAs between blood and tissue samples of patients with periodontitis and healthy controls using real time quantitative PCR technique. The present work was performed on samples got from 26 patients with periodontitis and 28 controls. Female/male ratio was 16/10 and 12/16 in cases and controls, respectively. Results There was no significant difference in the expressions of Linc0116, Linc00667, CDK6-AS1, FENDRR and DIRC3 genes between affected and unaffected tissues. However, expressions of Linc0116, Linc00667, CDK6-AS1, FENDRR and DIRC3 genes were significantly lower in the blood samples of patients when compared with control samples (Ratio of mean expression = 0.16, 0.14, 0.13, 0.10 and 0.14, respectively). Subsequently, we compared expressions of these lncRNAs between patients and controls in a sex-based manner. Expressions of Linc00667, FENDRR and DIRC3 genes were significantly lower in female patients compared with female controls (RME = 0.09, 0.07 and 0.10, respectively). Yet, there was no significant difference in expression of any of mentioned lncRNAs among male subgroups. Consistent with the similar levels of Linc0116, Linc00667, CDK6-AS1, FENDRR and DIRC3 in tissue samples of patients and controls, none of them could separate these two sets of samples. However, AUC values for of Linc0116, Linc00667, CDK6-AS1, FENDRR and DIRC3 expression levels in blood samples were 0.66, 0.72, 0.70, 0.72, 0.70 and 0.68, respectively with FENDRR having the best sensitivity value. Conclusion Taken together, lncRNAs might be involved in the pathologic events in the circulation of patients with periodontitis.

scholarly journals Pyrimidine 5’Nucleotidase I and II Activities in Acute Leukaemias

2004 ◽  
Vol 47 (2) ◽  
pp. 129-131 ◽  
Author(s):  
Haci Kemal Erdemli ◽  
Bahattin Adam ◽  
Nüket Bavbek
Keyword(s):  
High Performance Liquid Chromatography ◽  
High Performance ◽  
Mononuclear Cells ◽  
Control Group ◽  
Healthy Controls ◽  
Blood Samples ◽  
Significant Difference ◽  
Peripheral Mononuclear Cells ◽  
Design And Methods

Bacground and objective: Pyrimidine 5’nucleotidase I and II activities of peripheral mononuclear cells were studied to evaluate their role in diagnosis, assessment of therapy and follow up of remission in acute leukaemias. Design and methods: Blood samples were obtained from 40 untreated patients with acute lymphoblastic and myeloid leukaemia and 40 healthy controls, before the therapy and after remission. The correlation between the activity of the enzymes and the efficacy of therapy were established. The enzyme activities were measured by High-Performance Liquid Chromatography (HPLC), using the method described by Amici. For statistical analysis, Mann-Whitney U, Kruskal-Wallis and Wilcoxon methods were used. Results: Before the therapy, Pyrimidine 5’nucleotidase I levels in the leukaemic group were found to be significantly elevated when compared to the control group (p<0.001). Also Pyrimidine 5’nucleotidase II levels were significantly elevated before the therapy and during remission (p<0.02 and p<0.001 respectively). The isoenzyme activities were compared in patients who were in remission, who did not respond to therapy and in patients who died during the therapy, but no significant difference was found. Interpretation and conclusions: We concluded that, Pyrimidine 5’nucleotidase I and II activities can be used as markers for diagnosis and follow up of remission in patients with acute leukaemia. But, they can not have predictive value for prognosis.

Markers of Hemostatic Activation in Affected Coronary Arteries during Angioplasty

1994 ◽  
Vol 72 (05) ◽  
pp. 672-675 ◽  
Author(s):  
Nicolas W Shammas ◽  
Michael J Cunningham ◽  
Richard M Pomearntz ◽  
Charles W Francis
Keyword(s):  
Coronary Artery Disease ◽  
Coronary Artery ◽  
Venous Blood ◽  
Balloon Inflation ◽  
Blood Samples ◽  
Hemostatic System ◽  
Significant Difference ◽  
Hemostatic Markers ◽  
Artery Disease ◽  
High Doses

SummaryTo characterize the extent of early activation of the hemostatic system following angioplasty, we obtained blood samples from the involved coronary artery of 11 stable angina patients during the procedure and measured sensitive markers of thrombin formation (fibrino-peptide A, prothrombin fragment 1.2, and soluble fibrin) and of platelet activation ((3-thromboglobulin). Levels of hemostatic markers in venous blood obtained from 14 young individuals with low pretest probability for coronary artery disease were not significantly different from levels in venous blood or intracoronary samples obtained prior to angioplasty. Also, there was no translesional (proximal and distal to the lesion) gradient in any of the hemostatic markers before or after angioplasty in samples obtained between 18 and 21 min from the onset of the first balloon inflation. Furthermore, no significant difference was noted between angioplasty and postangioplasty intracoronary concentrations. We conclude that intracoronary hemostatic activation does not occur in the majority of patients during and immediately following coronary angioplasty when high doses of heparin and aspirin are administered.

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