scholarly journals Morphofunctional characteristics of mouse (Mus musculus musculus) liver on the application of various doses of nanostructural sapropel

2020 ◽  
Vol 17 ◽  
pp. 00079
Author(s):  
Ramil N. Faizrakhmanov ◽  
Yuliya V. Larina ◽  
Asiya M. Ezhkova ◽  
Vladimir O. Ezhkov ◽  
Ekaterina Semakina
Keyword(s):  
Body Weight ◽  
Lethal Dose ◽  
The Body ◽  
Intragastric Administration ◽  
Toxic Dose ◽  
Mus Musculus Musculus ◽  
Cervical Dislocation ◽  
Hematoxylin And Eosin ◽  
Flexible Probe ◽  
Mice Liver

The liver is considered to be the main organ in the processes of regulating metabolism, neutralizing toxins and maintaining the constancy of the internal environment of the body. The goal of the research was to study the morphofunctional state of the liver under the effect of different concentrations of nanostructured sapropel. The experiments were carried out on non-linear (outbred) white mice weighing 24.9 ± 1.8 g. Twelve mature males were allotted to four groups. Mice of the experimental groups I, II and III intragastrically through the atraumatic flexible probe were once injected with nanostructured sapropel (particle size of 45.0–180.0 nm) in the following doses: lethal – 3.0 g/kg of the body weight; toxic – 1.8 g/kg of the body weight and safe – 0.6 g/kg of the body weight. Mice of group IV served as a control one and received deionized water in the same way. The choice of liver as the organ for analyzing is justified by the fact that the liver did not have direct contact with sapropel nanoparticles in the process of its intragastric administration into the body of white mice. Four hours after the introduction of nanostructured sapropel, three mice from each group were killed by cervical dislocation. After preparation and staining with hematoxylin and eosin, identical pieces of the liver were evaluated using light microscopy. Histological studies have established that the introduction of a lethal dose of nanostructured sapropel caused hemodynamic vascular disorders; focal necrosis and necrobiosis of hepatocytes were also observed in the research. Furthermore, the research noted a migration of reticuloendotheliocytes to the centrolobular regions of the lobules and enhancement of their activity. The microstructure of the liver when introducing a toxic dose of nanostructured sapropel was characterized by moderate plethora of sinusoidal capillaries, deformation of hepatocytes, focal destruction with the development of karyopiknosis, karyorhexis and karyolysis. The study revealed the activation of reticuloendothelial cells. Liver histology when introducing a safe dose of nanostructured sapropel was characterized by the preservation of the integrity of the structural elements, polyploid (two- and multi-core) hepatocytes were identified in the periportal part of the lobes. The changes in the structural and functional state of the mice liver were found to be depending on the dose of the nanostructured sapropel.

scholarly journals Preclinical evaluation of the veterinary drug “Amoxidev 15” for its use in surgical and obstetric practice

2021 ◽  
Vol 23 (103) ◽  
pp. 109-115
Author(s):  
L.-M. Kostyshyn ◽  
R. Sachuk ◽  
Ye. Kostyshyn ◽  
O. Katsaraba
Keyword(s):  
Body Weight ◽  
Soft Tissues ◽  
Control Level ◽  
Subcutaneous Administration ◽  
The Body ◽  
Laboratory Animals ◽  
Veterinary Drug ◽  
Intragastric Administration ◽  
Toxicological Evaluation ◽  
White Rats

Suspension for injection “Amoxidev 15” is prescribed to fur-bearing animals (mink, fox), dogs and cats for the treatment of respiratory diseases (tonsillitis, tracheitis, pneumonia, bronchitis, rhinitis, sinusitis, bronchopneumonia), digestive (gastritis, enteritis, enteritis). genitourinary systems (nephritis, urethritis, urocystitis, mastitis, metritis, agalactia), musculoskeletal system (arthritis, osteoarthritis, joint injuries, tendonitis, hoof lesions), skin and soft tissues (eczema, dermatitis) caused by sensitive drug by microorganisms, including colibacillosis, streptococcus, bronchopneumonia, etc. Toxicological evaluation of the veterinary drug “Amoxidev 15” under the conditions of acute and subacute toxicological experiments on a model of white rats. According to the results of an acute toxicological experiment with intragastric administration of the drug “Amoxidev 15” white rats DL50 could not be calculated because the death of laboratory animals was not detected within 14 days after administration. The maximum administered dose (in absolute weight of the drug) was 20000.0 mg/kg body weight, which allows to refer the drug to class VI toxicity of relatively harmless substances (DL50 > 15000 mg/kg body weight), and the degree of safety to class IV – low-hazard substances (DL50 > 5000 mg/kg). According to the results of an acute toxicological experiment with subcutaneous administration of the drug “Amoxidev 15” white rats DL50 could not be calculated because the death of laboratory animals was not detected within 14 days after administration, the maximum dose was 5000.0 mg/kg body weight, therefore, the drug “Amoxidev 15” when administered subcutaneously by toxicity can be classified as class VI substances relatively harmless (DL50 Subcut > 4500.0 mg/kg). When administered subcutaneously to white rats, the drug “Amoxidev 15” under conditions of subacute toxicological experiment in doses of 0.1–1.0 ml/kg does not cause hemo-, hepato- and nephrotoxic effects on the body of laboratory animals, although 3-day administration of the drug in a dose 1.0 ml/kg body weight caused an increase in the activity of hepatospecific enzymes ALT and AST by 12.5 and 11.1 % (P < 0.05), respectively, relative to the control, which was restored to the control level 7 days after cessation.

Acute and sub-chronic toxicity of the aqueous extract of Ficus vogelii (Miq.) Miq. stem bark in rats

2021 ◽  
Vol 10 (2) ◽  
pp. 89-97
Author(s):  
EL Lappa ◽  
◽  
C Bogning Zangueu ◽  
EL Nguemfo ◽  
JJ Kojom Wanche ◽  
...  
Keyword(s):  
Body Weight ◽  
Aqueous Extract ◽  
Chronic Toxicity ◽  
Hematological Parameters ◽  
Lethal Dose ◽  
Relative Weight ◽  
Stem Bark ◽  
The Body ◽  
Female Rats ◽  
Male Rats

Ficus vogelii is a medicinal plant mainly found in tropical Africa and reported to treat inflammatory complaints. This study aims to evaluate the acute and sub-chronic toxicity of the aqueous extract of Ficus vogelii stem bark in wistar rats. For acute study, aqueous extract at a single dose of 5000 mg/kg body weight was administered to female rats and observed for 14 days. In the sub-chronic study, the extract was administered daily to both sex rats at the doses of 100, 200, 400, and 600 mg/kg body weight for 28 consecutive days. Body weight was measured weekly, while hematological, biochemical, and histopathological parameters were analyzed after euthanize. Aqueous extract of Ficus vogelii at all tested doses didn’t produced any mortality or significant change on the body weight and relative weight of rats on acute and sub-chronic studies. The lethal dose 50 was estimated greater than 5000 mg/kg (DL50˃5000 mg/kg). Hematological parameters were recorded non-significant in all treated rats. Aqueous extract at 600 mg/kg significantly changed transaminases and alkaline phosphatase activities, these changes were reversible in satellites. The concentrations of bilirubin was increased at 200 and 600 mg/kg in male rats, at 100, 400 mg/kg in female rats. The levels of lipids markers didn’t changed, except the significant decrease of LDL-cholesterol. Histological examination didn’t showed any change in the architecture of the liver and kidney of rats treated compared to control. Thus aqueous extract of Ficus vogelii stem bark didn’t produced adverse effects in rats after oral acute and sub-chronic treatment.

scholarly journals The Toxicology of Nickel Carbonyl

1908 ◽  
Vol 8 (5) ◽  
pp. 565-600 ◽  
Author(s):  
H. W. Armit
Keyword(s):  
Body Weight ◽  
Ganglion Cells ◽  
Lethal Dose ◽  
Fatty Degeneration ◽  
Iron Carbonyl ◽  
The Body ◽  
Selective Absorption ◽  
Nickel Carbonyl ◽  
Respiratory Surface ◽  
The Brain

Nickel carbonyl poisoning is a particular instance of nickel poisoning.The lethal dose of nickel varies according to the method of application. When applied by subcutaneous injection, the physical condition of the compound influences the rate of absorption and therefore relatively large quantities may be required. In rabbits, the lethal dose is about 7½ mgrs. per kilogram body weight under the most favourable conditions when applied subcutaneously. In cats it is about 12½ mgrs. per kilogram body weight. When applied intraperitoneally, the absorbing surface is considerably larger and consequently the dose required to kill is smaller. In rabbits it is less than 7 mgrs. When applied in the form of nickel carbonyl vapour one meets with the most favourable conditions for rapid absorption and the dose is therefore still smaller. Rabbits die after the absorption of between 3 and 4 mgrs., while cats die after absorbing about 8½ mgrs. per kilogram body weight.In the lungs, nickel carbonyl is dissociated and a nickel compound, probably the hydrated sub-carbonate, is deposited on the respiratory surface.The nickel is dissolved from the respiratory surface by the tissue fluids and is then taken up by the blood.Some of the nickel finds its way directly through the lymphatic channels into the bronchial glands.In the dissolved condition, the nickel enters into complex combination with some constituent of the body.The nickel is carried by the blood to the tissues, but a selective absorption is exercised by the brain and adrenals. In the case of other forms of nickel poisoning, the lungs also exert this specific selection. The nickel only stays for a short time in these organs.The specific pathological changes which are produced by nickel in these organs are primarily a degeneration of the endothelial cells of the capillary vessels. It is possible that some further primary action is exercised on the ganglion cells in the brain and on the parenchyma cells of the adrenals.The haemorrhages follow as the result of the fatty degeneration of the vessel walls and secondary changes result from the effects of the haemorrhages.The nickel is excreted by the kidneys and intestines.The method of poisoning with iron carbonyl is similar to that of nickel poisoning, but the amount necessary to kill in the former case is larger.Iron carbonyl poisoning like nickel carbonyl poisoning is merely a specific instance of metallic poisoning.Iron acts in a similar manner to nickel on the walls of capillary vessels, but no evidence of selection by any special tissues was obtained.Cobalt has a toxicological action which is identical to that of nickel. The lethal dose however is higher than that of nickel and lower than that of iron.After the inhalation of a quantity of nickel or iron carbonyl which is greater than the minimum required to kill, no form of treatment was found to avert death.It is with much pleasure that I again express my gratitude to Dr Ludwig Mond, F.R.S., for having rendered this investigation possible, by defraying all the expenses, and by giving me the benefit of his advice.I further desire cordially to thank Dr C. J. Martin, F.R.S., and the other members of the Staff of the Lister Institute, who have at all times been ready and willing to assist me. The work has necessitated incursions into several branches of science, and has required the acquisition of a variety of methods. I have made free use of their kind collegiality and am glad to avail myself of this opportunity of recording my indebtedness.The literature of the subject has been given in Part I, q.v.

scholarly journals Changes in proteome profiles of rat liver microsomes induced by silicon dioxide nanoparticles

2015 ◽  
Vol 61 (1) ◽  
pp. 92-98
Author(s):  
O.N. Tananova ◽  
E.A. Arianova ◽  
I.V. Gmoshinskii ◽  
I.Yu. Toropygin ◽  
E.V. Khryapova ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Body Weight ◽  
Liver Microsomes ◽  
The Body ◽  
Mass Spectrometry Analysis ◽  
Control Group ◽  
Intragastric Administration ◽  
2D Electrophoresis ◽  
Rat Liver Microsomes ◽  
Mass Spectrometry Identification

The effect of daily intragastric administration of an aqueous dispersion of silicon nanoparticles (NPs) (the dose range from 1.0 mg/kg to 100 mg/kg body weight for 28 days) to rats on the proteomic profile of liver microsomes has been investigated by 2D-electrophoresis followed by subsequent mass spectrometry identification. The liver microsomal fraction was isolated by differential centrifugation and its protein composition was analyzed by 2D-polyacrylamide gel electrophoresis. Identification of protein spots was carried out using MALDI-TOF mass spectrometric analysis. The mass spectrometry analysis revealed the protein GRP78 (78 kD glucose-regulated protein precursor), belonging to the family of heat shock proteins. This protein present in animals of the control group was not detected in NP-treated rats of group 2 (1 mg/kg body weight/day) and group 3 (10 mg/kg body weight/day). This protein predominantly localized in the liver cell endoplasmic reticulum and plasma membrane has the chaperone biological activity. Possible mechanisms of the effects of engineered nanoparticles on biosynthetic processes in the body are discussed.

scholarly journals Curcumin ameliorates atrophy of seminal vesicle via reduction of oxidative stress in castrated mice

PeerJ ◽  
2019 ◽  
Vol 7 ◽  
pp. e7192 ◽  
Author(s):  
Rui Li ◽  
Hao Li ◽  
Ke Rao ◽  
Kang Liu ◽  
Yan Zhang ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Body Weight ◽  
Seminal Vesicle ◽  
Serum Testosterone ◽  
The Body ◽  
Intragastric Administration ◽  
Curcumin Treatment ◽  
Seminal Vesicle Weight ◽  
High Level ◽  
And Function

Background The growth and function of seminal vesicle are dependent on androgen. This study was conducted to investigate the role of oxidative stress in castration-induced seminal vesicle atrophy and to explore the effects of curcumin, an antioxidant extracted from rhizome of turmeric, on seminal vesicle of castrated mice. Methods C57BL/6J mice were randomly divided into three groups: control, castration, and castration with curcumin (n = 10 for each group). After surgical castration, mice in the curcumin treatment group received intragastric administration of curcumin at 100 mg/kg body weight for 4 weeks, whereas mice in the other two groups were treated with olive oil. After that, the body weight, seminal vesicle weight and serum testosterone of mice were measured. Apoptosis and oxidative stress levels in seminal vesicle were also determined. Results After castration, both the weight and size of seminal vesicle decreased dramatically. The expression of three NADPH oxidase (NOX) subtypes: NOX1, NOX2 and NOX4, increased in seminal vesicle of castrated mice, resulting in high level oxidative stress. The ratio of Bax to Bcl-2 was also elevated after castration, accompanied by enhanced caspase3 activity. Additionally, castration increased the number of apoptotic cells in seminal vesicle. Curcumin treatment could inhibit the expression of NOX1, NOX2 and NOX4, decreasing oxidative stress and apoptosis. The atrophy of seminal vesicle caused by castration was ameliorated by curcumin. Conclusion Castration could cause atrophy of seminal vesicle probably via inducing oxidative stress. Curcumin treatment could reduce the oxidative stress in seminal vesicle by decreasing the expression of NOX1, NOX2 and NOX4, thereby ameliorating apoptosis and atrophy of seminal vesicle. Oxidative stress might play a role in castration-induced seminal vesicle atrophy.

Adverse effects of lactational exposure to chlorpyrifos in suckling rats

2009 ◽  
Vol 29 (2) ◽  
pp. 77-92 ◽  
Author(s):  
SA Mansour ◽  
AH Mossa
Keyword(s):  
Body Weight ◽  
Adverse Effects ◽  
Body Weight Gain ◽  
Lethal Dose ◽  
Daily Intake ◽  
Oral Route ◽  
The Body ◽  
Lactational Exposure ◽  
Histopathological Alterations ◽  
Liver And Kidney

The present study was undertaken to evaluate the oxidative damage, biochemical and histopathological alterations in sucking rats whose mothers were exposed to the insecticide chlorpyrifos (CPF). Dams were administered CPF, via oral route. Doses equalled 0.01 mg kg—1 body weight (b.wt.; acceptable daily intake, ADI), 1.00 mg kg—1 b.wt. (no observed adverse effects level, NOAEL) and 1.35 mg kg—1 b.wt. (1/100 lethal dose [LD50]) from postnatal day 1 until day 20 after delivery. At two high doses of CPF, the body weight gain and relative liver and kidney weight of suckling pups were significantly decreased. Exposure of the mothers to CPF caused increase in lipid peroxidation (LPO) and decrease in superoxide dismutase (SOD) and glutathione-s-transferase (GST) in lactating pups. CPF altered the level of the marker parameters related to the liver and kidneys. Consistent histological changes were found in the liver and kidneys of the subjected pups, especially at the higher doses. The results suggested that the transfer of CPF intoxication through the mother’s milk has resulted in oxidative stress and biochemical and histopathological alterations in the suckling pups. The data of this study may be considered as a contribution to the problem of lactational transfer of the relatively less persistent OP pesticides, such as CPF.

scholarly journals The determination of the minimal lethal dose of various toxic substances and its relationship to the body weight warm-blooded animals, together with considerations bearing on the dosage of drugs

1914 ◽  
Vol 87 (595) ◽  
pp. 319-330 ◽  
Keyword(s):  
Body Weight ◽  
Blood Volume ◽  
Body Surface ◽  
Lethal Dose ◽  
The Body ◽  
Toxic Substances ◽  
Production Distribution ◽  
Close Relationship ◽  
Series Of Experiments

In the course of investigations on the production, distribution, and rate of disappearance in the body of immune substances, we were occupied in 1908 and previous years with a series of experiments on agglutinins, and we arrived at conclusions pointing to their close relationship to the blood and blood-forming organs (1, 2). In association with these inquiries, one of us (G. D.), together with W. Ray, published a communication on the relation­ship between the blood volume and the distribution of agglutinins within the circulation (3). It was there shown that the concentration of this substance (agglutinin) in the blood after inoculation into an animal was proportional to the body surface of the animal concerned, and was thus approximately proportional to the two-thirds power of the weight. Hence was deduced the conclusion that the blood volume of the animals examined was proportional to their body surface.

scholarly journals Toxicity Profile of a Nutraceutical Formulation Derived from Green MusselPerna viridis

2014 ◽  
Vol 2014 ◽  
pp. 1-14 ◽  
Author(s):  
Kajal Chakraborty ◽  
Deepu Joseph ◽  
Selsa J. Chakkalakal
Keyword(s):  
Body Weight ◽  
Water Consumption ◽  
Lethal Dose ◽  
Clinical Signs ◽  
The Body ◽  
Toxicity Study ◽  
Perna Viridis ◽  
Toxicity Profile ◽  
Subchronic Toxicity ◽  
Green Mussel

The short-term (acute) and long-term (subchronic) toxicity profile, mean lethal dose 50 (LD50), and no-observed-adverse-effect level (NOAEL) of a nutraceutical formulation developed from green musselPerna viridis, which showedin vitroandin vivoanti-inflammatory properties, were evaluated in the present study. The formulation was administered to the male and female Wistar rats at graded doses (0.5, 1.0, and 2.5 g/kg body weight) for two weeks of acute toxicity study and 0.5, 1.0, and 2.0 g/kg body weight for 90 days in subchronic toxicity study. The LD50, variations in clinical signs, changes in body weight, body weight, food/water consumption, organ weight (liver, kidney, spleen, and brain), hematology, serum chemistry, and histopathological changes were evaluated. The LD50of the formulation was 5,000 mg/kg BW. No test article related mortalities as well as change in body weight, and food and water consumption were observed. No toxicity related significant changes were noted in renal/hepatic function, hematological indices, and serum biochemical parameters between the control and treated groups. Histopathological alterations were not observed in the vital organs of rats. The subchronic NOAEL for the formulation in rats is greater than 2000 mg/kg. This study demonstrated that the green mussel formulation is safe to consume without any adverse effects in the body.

scholarly journals Acute and subacute toxicity of the drug based on tylosin tartrate

2019 ◽  
Vol 21 (94) ◽  
pp. 97-102
Author(s):  
I. P. Patereha ◽  
V. I. Kushnir ◽  
M. I. Zhyla ◽  
O. M. Dubin
Keyword(s):  
Body Weight ◽  
Kidney Function ◽  
Therapeutic Dose ◽  
The Body ◽  
Water Soluble ◽  
Clinical State ◽  
Intragastric Administration ◽  
Subacute Toxicity ◽  
Γ Globulins ◽  
High Level

The article presents the results of a study of the acute and subacute toxicity of the drug TILOTAR (a water-soluble powder for oral administration) made on the basis of tilmicosin. As a result of the research it was found that the drug “TILOTAR” refers to the 4th class of toxicity, that is, to low-toxic substances. After intragastric administration of the drug to white mice and rats at doses of 2500, 5000, 7500 mg/kg, all animals remained alive. Short-term inhibition was recorded in laboratory animals, which were given the drug at the maximum dose. Further changes in the clinical state of the animals of the experimental groups were not observed. The study of subacute toxicity of the drug “TILOTAR” showed that 14-day administration of a therapeutic dose of the drug leads to a decrease in erythropoiesis, the appearance of old forms of erythrocytes, damage to hepatocytes, the appearance of eosinophils, γ-globulins, a decrease in protein biosynthesis, increased kidney function. The introduction of a 10-fold dose within 14 days reduced the body weight of the rats, leading to abnormalities in the liver and kidneys, and a decrease in the mass of the spleen. An increase in urea blood concentration and a decrease in creatinine may indicate impaired renal function in animals that were given a tenfold dose of the drug, and an increase in the level of α- and γ-globulins against the background of a decrease in body weight of rats indicates an overload of the body’s immune system. In rats of the therapeutic group, homeostasis was maintained by enhancing kidney function, accompanied by a decrease in the number of erythrocytes due to young forms and the appearance of mature erythrocytes with a high level of hemoglobin (MCH), some destruction of hepatocytes (high level of alkaline phosphatase activity) while reducing the amount of albumin, an increase in relative levels of α- and γ-globulins. In the conducted experiments it was established that the macroscopic and microscopic structure of the internal organs of rats, while studying the subacute toxicity of the drug “TILOTAR”, was preserved, no macroscopic changes were revealed. In rats receiving a 10-fold therapeutic dose of the drug for 14 days, focal protein dystrophy of the liver and kidneys was histologically established.

scholarly journals INFLUENCE OF COMBINED INJECTION OF THYROXIN AND PROPYLTHIOURACILUM ON SCTRUCTURAL INDICATORS OF RENAL PARENCHYMA

2017 ◽  
Vol 21 (1) ◽  
pp. 57-67
Author(s):  
S. I. Dolomatov ◽  
V. G. Sipovski ◽  
N. Y. Novikov ◽  
I. N. Kasich ◽  
I. V. Myshko ◽  
...  
Keyword(s):  
Body Weight ◽  
Structural Changes ◽  
White Male ◽  
Kidney Tissue ◽  
Renal Parenchyma ◽  
Male Rats ◽  
Intragastric Administration ◽  
Tissue Samples ◽  
Hematoxylin And Eosin

THE AIM: to study of the dynamics of structural changes in renal parenchyma of rats exposed to long-term combined effects of thyroxine and propylthiouracilum (PTU). MATHERIAL AND METHODS – studies were performed on mongrel white male rats weighing 250-300g. Hyperthyroidism was caused by daily intragastric administration of thyroxine (T4) in amount of 50g per 100g of body weight over 30 days. On the first day of the experiment animals were divided into 2 groups. Animals of the first group (n = 25) received only T4. The rats of the second group (n = 25) were administrated propylthiouracilum and T4 daily. PTU was administered intragastric in amount of 1 mg per 100g of body weight. Kidney tissue samples were collected on the 10th, 20th and 30th days of the experiment. In addition, there were collected kidney tissue samples of the animals treated with only T4 after 20 days after cessation of hormone. Obtained tissue samples were fixed and treated by the usual method, followed by filling in paraffin. Sections were stained with hematoxylin and eosin. RESULTS – it was established that course of experimental hyperthyroidism leads to significant structural abnormalities of the renal parenchyma. Leading features of kidneys pathology at a hyperthyroidism are rough structural damages of the nephron tubular epithelium. CONCLUSIONS – combined administration in rats of thyroxin and propylthiouracilum has weakly expressed beneficial effect by limiting the development of structural damages to the renal parenchyma and clot formation. 

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