scholarly journals Technological properties of cow milk with the addition in their diet of silage prepared with bio-preservative “GreenGrass 3×3”

2021 ◽  
Vol 37 ◽  
pp. 00072
Author(s):  
Sergey V. Karamaev ◽  
Natalya V. Soboleva ◽  
Anna S. Karamaeva ◽  
Nikolay A. Mironov
Keyword(s):  
Mass Fraction ◽  
General Structure ◽  
Whey Proteins ◽  
Milk Proteins ◽  
The Body ◽  
Cow Milk ◽  
Technological Properties ◽  
Holstein Breed ◽  
Lactating Cows ◽  
Daily Milk Yield

The completeness of the provision of the body of highly productive cows with the necessary set of nutrients depends on the set of feeds included in their diet, as well as on their quality. The purpose of the research is to study the effect of silage with the biological preservative “GreenGrass 3×3” on the technological properties of milk from cows of Holstein and Ayrshire breeds. It is found that the morphological and biochemical composition of the blood in the animals of the experimental groups significantly improves approaching the optimal parameters. Feeding lactating cows with silage in feed mixture from lucerne prepared with bio-preservative increases the average daily milk yield by 20.5% in the Holstein breed and by 26.9% in the Ayrshire breed. The quality of milk is improved by the increase in the mass fat fraction by 0.10-0.13% respectively, the mass protein fraction - by 0.10-0.14%, including casein - by 0.14-0.19%. In the general structure of milk proteins, the mass fraction of casein increases, while the mass fraction of whey proteins, on the contrary, decreased by 2.0-2.2%. It is very important that the proportion of γ-caseins, which do not clot under the influence of rennet, decreased by 0.5-0.9% in the test samples. As a result, the duration of clotting by rennet decreased by 13.8-12.1% and the loss of dry matter with whey - by 1.3-2.1%, the yield of casein clot increased by 5-4% and its density increased by 0,37-0.22 g / cm2. All these aspects show the increase in the technological properties of milk in cows from the experimental groups.

scholarly journals Post translational modifications of milk proteins in geographically diverse goat breeds

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
P. K. Rout ◽  
M. Verma
Keyword(s):  
Protein Function ◽  
Whey Proteins ◽  
Milk Proteins ◽  
Goat Milk ◽  
Peptide Sequence ◽  
Cow Milk ◽  
Post Translational Modification ◽  
Post Translational Modifications ◽  
Functional Roles ◽  
Dpp Iv

AbstractGoat milk is a source of nutrition in difficult areas and has lesser allerginicity than cow milk. It is leading in the area for nutraceutical formulation and drug development using goat mammary gland as a bioreactor. Post translational modifications of a protein regulate protein function, biological activity, stabilization and interactions. The protein variants of goat milk from 10 breeds were studied for the post translational modifications by combining highly sensitive 2DE and Q-Exactive LC-MS/MS. Here we observed high levels of post translational modifications in 201 peptides of 120 goat milk proteins. The phosphosites observed for CSN2, CSN1S1, CSN1S2, CSN3 were 11P, 13P, 17P and 6P, respectively in 105 casein phosphopeptides. Whey proteins BLG and LALBA showed 19 and 4 phosphosites respectively. Post translational modification was observed in 45 low abundant non-casein milk proteins mainly associated with signal transduction, immune system, developmental biology and metabolism pathways. Pasp is reported for the first time in 47 sites. The rare conserved peptide sequence of (SSSEE) was observed in αS1 and αS2 casein. The functional roles of identified phosphopeptides included anti-microbial, DPP-IV inhibitory, anti-inflammatory and ACE inhibitory. This is first report from tropics, investigating post translational modifications in casein and non-casein goat milk proteins and studies their interactions.

Dynamics of productivity and indicators of functional homeostasis of lactating cows when supplements based on natural zeolites enriched with a biocomplex of free L-amino acids are introduced into the diet

2022 ◽  
pp. 59-66
Author(s):  
K. Leshchukov ◽  
V. Masalov ◽  
N. Yarovan ◽  
M. Kotаlnikova ◽  
A. Mamaev
Keyword(s):  
Amino Acids ◽  
Weight Loss ◽  
Energy Balance ◽  
Mass Fraction ◽  
Dry Matter ◽  
Negative Energy ◽  
Feed Additive ◽  
Lactating Cows ◽  
Daily Milk Yield ◽  
Daily Milk

Purpose: to study the effect of feeding lactating cows with a feed additive enriched with a biocomplex of free L-amino acids of plant origin on the indicators of functional homeostasis, productivity and quality characteristics of milk.Materials and methods. Research and production tests were carried out on the basis of the OS "Streletskaya" branch of the Federal State Budgetary Scientific Institution "Federal Research Center of Legumes and Groats". To conduct scientific and production tests, 2 groups of cows of 3-4 lactation were formed, 70 heads each. The groups were formed on the principle of analogous pairs.The cows of the experimental group, starting from the second day after calving, in the morning, once a day, received a granulated feed additive "ZEO-AMINO" (Russia) fraction 0.2-0.7 mm at the rate of 2% of the dry matter of the ration daily for the first 100 days lactation. Experimental animals were monitored daily. Clinical examination of animals was carried out with obligatory control of the general condition, thermometry, counting of respiratory movements and rumen contractions, assessment of the udder condition. The productivity of cows was determined according to the results of control milking. Morphological and biochemical blood tests were performed.Results. It was revealed that the daily use of a granular feed additive of fraction 0.2-0.7 mm, starting from the second day after calving, once a day at the rate of 2% of the dry matter of the diet during the first 100 days of lactation, contributes to an increase in the average daily milk yield of cows on average by 15.9%; by 7 months lactation, an increase in the average daily milk yield was established by an average of 18.3%. It was found that feeding the supplement to cows after calving due to better absorption of nitrogen in the diet and accumulation of proteins in the animal's body allows partially neutralizing the negative consequences of negative energy balance after calving, and contributes to a more intensive recovery of live weight loss, which is reliably confirmed by indicators of animal productivity. The results of a biochemical blood test suggest that the use of a feed supplement in obtaining milk has a positive effect on the assimilation of the diet, metabolism and assimilation processes in the body, activates a number of vital functions during the normal course of physiological processes and ensuring functional homeostasis, which ultimately determines increasing the milk production of animals. Analysis of the quality indicators of milk allows us to conclude that the mass fraction of fat when feeding the additive increased by an average of 0.12 abs. %; the mass fraction of protein increased by an average of 0.22 abs.%.Conclusion. To increase milk productivity, reduce the negative effects of negative energy balance after calving, and more intensively restore body weight loss, as well as increase the efficiency of using feed proteins and normalize metabolic processes, it is recommended that daily use of the feed granulated dietary supplement "ZEO-AMINO" fraction in the diets of dairy cows 0.2-0.7 mm at the rate of 2% of the dry matter of the diet, starting from the second day after calving, once a day during the first 100 days of lactation.

scholarly journals Increasing the biological value of the curd product

2018 ◽  
Vol 80 (3) ◽  
pp. 124-127
Author(s):  
E. I. Dobriyan ◽  
A. M. Ilina ◽  
T. A. Medvedeva
Keyword(s):  
Carbohydrate Metabolism ◽  
Milk Fat ◽  
Nutritive Value ◽  
Wide Spectrum ◽  
Whey Proteins ◽  
Milk Proteins ◽  
The Body ◽  
Diabetic Patients ◽  
Biological Value ◽  
Test Intensity

The article is devoted to the improvement of quark product biological value in order to impart the properties adequate to specifity and nutrient status for the group of population with abnormal carbohydrate metabolism. Diabetes comprises the most widespread chronic diseases in the world. The reason for the number of diabetic patients growth bears the complex character but this growth is conditioned mainly by malnutrition. Correctly chosen diet adequate to the level and character of metabolic disorders is the important prophylactic factor. Milk products among which quark and quark products due to high amount of easily digestible protein are very Important in nutrition of the patients suffering from abnormal carbohydrate metabolism. The marketing analysis of this group nutritive value presented in the marked has been carried out. The obtained results show that the manufactures quark products are characterized by low amount of crude protein (from 4.5 to 6.0%) and high carbohydrates content ( from 13,0 to 18.0%) including sucrose (from 7,5 to 12,0%. One of the main requirements to dietetic therapy of the patients suffering from diabetes is drastic restriction and exclusion from food allowance easily digestible refined carbohydrates. The aim of the work is to improve biological value of the quark product by replacement of sucrose by stevia and enrichment it by whey protein. Among sugar substitute of natural origin is stevia, which besides the ability to reduce sugar possesses the wide spectrum of positive physiological impact on the body. The influence of milk components on the expressed after-taste of stevia and the possibility to level it has been studied. It was stated that protein has no impact on stevia after-taste but milk fat partially levels metallic taste and promotes the test intensity. It was stated that the best product organoleptic indices were fixed after introduction of 0.027% of stevia. Milk proteins are food irreplaceable components and mainly whey proteins characterized by high biological value conditioned by their specificity and balanced amino acid composition. But meanwhile commercial samples of quark products contained not more than 0,05% of whey proteins . The possibility to improve the biological value of the product by enrichment with whey proteins has been studied.

Comparative effectiveness of the use of wet flattened corn grain in the rations of lactating cows

2021 ◽  
pp. 25-32
Author(s):  
K. V. Kireeva ◽  
I. A. Pushkaryev ◽  
N. M. Kostomakhin ◽  
T. A. Litvintseva
Keyword(s):  
Milk Yield ◽  
Practical Interest ◽  
The Body ◽  
Control Group ◽  
Lactating Cows ◽  
Daily Milk Yield ◽  
Experimental Group ◽  
Corn Grain ◽  
Corn Grains ◽  
Daily Milk

In order to effectively provide the body of cows with energy during the increasing the milk yield without the risk of acidosis, progressive animal breeders of the Altai Territory include wet flattened corn grain in the composition of concentrated feed. The question of the expediency and economic effectiveness of using wet flattened corn grain for cows of various physiological groups – first-calf heifers and mature animals continues to be debatable. Therefore, a comparative assessment of two options for feeding wet flattened corn grain to cows under the conditions of the Altai Territory is considered promising in modern animal husbandry and is of great practical interest. The purpose of the work was to identify the optimal and economically feasible option for using wet flattened corn grain in the rations of lactating cows. When using wet flattened corn grains in an amount of 7,1 % of the nutritional value of the ration the first-calf heifers during the period of increasing the milk yield, productivity has increased by 20,9 %. The average daily milk yield was 17,3 kg, which significantly exceeded the indicator of the control group (P > 0,95). The inclusion of wet flattened corn grains in the ration of mature cows in the amount of 1 kg for 30–35 days before calving, then 4,5 kg from 15 to 75 days after calving allows us to get the average daily milk yield of 30 kg or 20,3 % higher than that of the control group of herdmates (P > 0,95). The effectiveness of the use of wet flattened corn grain had significant indicators: the total profit from the sale of dairy products of the first-calf heifers of the experimental group amounted to 16 251,3 rubles/head, and from the sale of milk of mature cows of the experimental group it was 30 346,43 rubles/head.

scholarly journals Are Faba Bean and Pea Proteins Potential Whey Protein Substitutes in Infant Formulas? An In Vitro Dynamic Digestion Approach

Foods ◽  
2020 ◽  
Vol 9 (3) ◽  
pp. 362
Author(s):  
Linda Le Roux ◽  
Olivia Ménard ◽  
Raphaël Chacon ◽  
Didier Dupont ◽  
Romain Jeantet ◽  
...  
Keyword(s):  
Faba Bean ◽  
Whey Proteins ◽  
Milk Proteins ◽  
Protein Digestibility ◽  
Cow Milk ◽  
Partial Substitution ◽  
Infant Formulas ◽  
Pea Proteins

Infant formulas (IFs) are used as substitutes for human milk and are mostly based on cow milk proteins. For sustainability reasons, animal protein alternatives in food are increasingly being considered, as plant proteins offer interesting nutritional and functional benefits for the development of innovative IFs. This study aimed to assess how a partial substitution (50%) of dairy proteins with faba bean and pea proteins influenced the digestibility of IFs under simulated dynamic in vitro digestion, which were set up to mimic infant digestion. Pea- and faba bean-based IFs (PIF and FIF, respectively) have led to a faster aggregation than the reference milk-based IF (RIF) in the gastric compartment; that did not affect the digesta microstructure at the end of digestion. The extent of proteolysis was estimated via the hydrolysis degree, which was the highest for FIF (73%) and the lowest for RIF (50%). Finally, it was apparent that in vitro protein digestibility and protein digestibility-corrected amino acid score (PDCAAS)-like scores were similar for RIF and FIF (90% digestibility; 75% PDCAAS), but lower for PIF (75%; 67%). Therefore, this study confirms that faba bean proteins could be a good candidate for partial substitution of whey proteins in IFs from a nutritional point of view, provided that these in vitro results are confirmed in vivo.

scholarly journals КЛІНІКО–БІОХІМІЧНИЙ СТАТУС ДІЙНИХ КОРІВ ПАФ «НЕФЕДІВСЬКЕ» КАМ’ЯНЕЦЬ–ПОДІЛЬСЬКОГО РАЙОНУ ХМЕЛЬНИЦЬКОЇ ОБЛАСТІ

2016 ◽  
Vol 18 (3(71)) ◽  
pp. 37-40
Author(s):  
О.M. Koltun ◽  
V.I. Rusyn
Keyword(s):  
Dairy Cows ◽  
Mineral Metabolism ◽  
Bovine Milk ◽  
Hypochromic Anemia ◽  
Inorganic Phosphorus ◽  
The Body ◽  
Black And White ◽  
Lactating Cows ◽  
Biochemical Status ◽  
Daily Milk Yield

These results of clinical and biochemical status of the herd of dairy cows PAF «Nefedivske» Kamenets–Podolsk district of Khmelnitsky region. The aim was to determine the clinical and biochemical status of the herd of dairy cows and its results to develop measures correcting the violations. Work performed at the PAF «Nefedivske» Kamenets–Podolsk district of Khmelnitsky region. The object of the research were lactating cows, black and white breeds, ages 4 – 6 years and daily milk yield 16 – 18 liters of milk.Survey livestock lactating cows clinically infected animals, which noted the following symptoms: drowsiness animals, reducing distortion and appetite, dullness and disheveled hair cover; delay molting; decrease skin resilience, dry it and thickening; anemic visible mucous membranes; reducing the number of periods and bovine milk production. Serum lactating cows found high content of inorganic phosphorus and iron, and low content of copper and zinc, which constitutes a violation of mineral metabolism. Patient’s hemocytopoesis animals found violations that characterized olihochromemia, decreased hematocrit values and average concentrations of hemoglobin, indicating the development of hypochromic anemia. According to the results of biochemical blood tests in lactating cows installed hipoproteinemia, hipoalbuminemia, and high activity of transaminases and alkaline phosphatase, indicating a violation of metabolic processes in the body sick animals. These results indicate the need for the development and management of correcting the violations of metabolism in dairy cows.

scholarly journals Increasing Economic Efficiency of Cow Milk Production in the First Phase of Lactation

2019 ◽  
Vol 8 (12) ◽  
pp. 4072-4074
Keyword(s):  
Milk Production ◽  
Economic Efficiency ◽  
Dry Matter ◽  
Cow Milk ◽  
Feed Supplement ◽  
Feed Mixture ◽  
Lactating Cows ◽  
Service Period ◽  
Daily Milk Yield ◽  
Daily Milk

The article considers the economic efficiency of the use of the PassPro Balance feed product for lactating cows based on the increase of their productivity and reproductive qualities. The study has found that the use of the PassPro Balance feed supplement in the ration of Simmental cows during milking period increases the consumption of feed mixture by 0.9% and dry matter – by 5.4%, average daily milk yield – by 29.2% (P<0.01) and profitability level of milk production – by 7.8%, as well as reduces the service period by 5.0% and the consumption of concentrates for the production of 1 kg of basic fat milk by 22.8%.

scholarly journals MAJOR GOAT MILK PROTEIN: SEPARATION AND CHARACTERIZATION BY “LAB-ON-A-CHIP” MICROFLUIDIC ELECTROPHORES

2018 ◽  
Vol 35 (2) ◽  
Author(s):  
ALESSA SIQUEIRA DE O. DOS SANTOS ◽  
VANEIDA MARIA MEURER ◽  
FABIANO FREIRE COSTA ◽  
IGOR MOURA DE PAIVA ◽  
GISELE NOGUEIRA FOGAÇA ◽  
...  
Keyword(s):  
Protein Separation ◽  
Whey Proteins ◽  
Milk Proteins ◽  
Goat Milk ◽  
Lab On A Chip ◽  
Cow Milk ◽  
Total Percentage ◽  
Chip Technology ◽  
Sds Page ◽  
Β Lactoglobulin

This work presents the electrophoretic profile of goat and cow milk samples and their mixtures using microfluidic and conventional electrophoresis. The microfluidic method allowed the separation of the major caseins from milk, excepting the goat κ-casein.  Besides, the major whey proteins were separated with perfect distinction of A and B β-lactoglobulin variants. Comparing to SDS-PAGE, a variation in the molecular weight was observed in all milk proteins. However, A and B β-lactoglobulin variants could not be isolated using SDS-PAGE. Although urea-PAGE did not show high resolution among whey proteins, γ-, κ-, β-, and α-caseins were clearly identified. This method also showed a lower limit detection of cow milk in mixture samples than the "lab-on-a-chip" electrophoresis. In both methods, the highest linearity obtained from plotting total percentage against cow milk concentration was observed by using cow αs1-casein (R2 = 0.986 and R² = 0.973). This result indicates that microfluidic electrophoresis is an effective tool to detect the presence of some proteins in goat and cow milk, and in mixtures. Microfluidic chip technology might will complement the current methods for analyzing milk proteins, highlighting its speed amount of reagents and whey protein separation, which showed a better result than urea or SDS-PAGE

Analysis of the surplus of nutrients in ration of lactating cows with different methods of housing

2021 ◽  
pp. 4-11
Author(s):  
G.E. Akifieva ◽  
N.N. Novikova
Keyword(s):  
Dry Matter ◽  
Metabolic Diseases ◽  
Live Weight ◽  
The Body ◽  
Energy Output ◽  
High Concentration ◽  
Protein Ratio ◽  
High Productivity ◽  
Lactating Cows ◽  
Daily Milk Yield

Complete feeding is one of the most important factors that ensure the success of breeding work; it is the basis for increasing the productivity of animals, improving existing and breeding new breeds and types. In order to obtain high productivity from animals in accordance with their genetic potential it is necessary to ensure that animals consume more dry matter in rations with a variety of high-quality feed with a high concentration of energy and nutrients in the dry matter. The purpose of the research was to study the nutritional value of feeding rations in breeding farm with different technologies of cows housing in accordance with the feeding standards. The experiment has been carried out in the breeding farm of CJSC “Azovskoye” in the Azov district in the Omsk region. The results of studies of the ration of lactating cows of Red Steppe breed with a live weight of 500–550 kg and a daily milk yield of 19,5 kg of milk with different methods of housing (tie-up housing and free-stall housing) have been presented. It has been established that the farm ration did not provide for the specifics of the housing technology and consists of energy, protein and expensive feed with insufficient content of carbohydrates and macro elements (phosphorus). The proposed ration takes into account the peculiarities of the technology of animals housing, equalizes the indicators of the sugar-protein ratio, as well as phosphorus to calcium by reducing concentrated, coarse feed and introducing grain molasses, as well as phosphorus additives. The balanced ration provides for a large energy output of 9,97 MJ and prevents a number of possible metabolic diseases in the body associated with a violation in the feeding system of lactating cows.

scholarly journals INFLUENCE OF ARGENTUM NANOPARTICLES ON ECONOMIC AND PHYSIOLOGICAL-BIOCHEMICAL INDICATORS OF LACTATING COWS DURING LONG FEEDING WITH TRADITIONAL AND TRANSGENIC SOYBEAN

2020 ◽  
Vol 11 (4) ◽  
pp. 61-69
Author(s):  
N. M. Omelchenko
Keyword(s):  
Milk Yield ◽  
Animal Feed ◽  
Genetically Modified ◽  
Experimental Period ◽  
The Body ◽  
Transgenic Soybean ◽  
Lactating Cows ◽  
Corrective Effect ◽  
Daily Milk Yield ◽  
Daily Milk

Ukraine is among the world's top ten soybean-producing countries. The significant prevalence of Roundup transgenic soybean varieties increases the likelihood of it becoming part of farm animal feed. Accordingly, it is important to study the effect of genetically modified soy on the body of animals and to develop ways to neutralize the deviations caused by its action. A study of the effect of traditional and transgenic soy on reproductive function, physiological, biochemical and economic parameters of lactating cows. Cows' feed intake with the addition of conventional soya had no significant effect on litter size. In the group of animals that consumed transgenic soya, there was a tendency for a reduction in the total number of newborns and an increase in the number of stillborn calves. The use of transgenic soybeans by lactating cows promotes the increase of ALT activity and alkaline phosphatase concentration. Feeding genetically modified soy to cows does not change the chemical composition of milk and does not significantly affect daily milk yield. The addition of Nano argentum citrate to the diet of animals increased the average daily milk yield throughout the experimental period. At 10 days of Nano argentum use, the cows of the experimental group had higher daily milk yields by 10.5 %, and at 20 and 30 days – by 7.5 and 6.4 %, respectively. The corrective effect of the drug Nano argentum on physiological and biochemical parameters in animals that have used transgenic soy for a long time has been established. This indicates the detoxifying properties of the drug and allows to recommend it as a means of preventive protection of animals whose diet may include genetically modified soybeans.

Sign in / Sign up

Export Citation Format

Share Document