scholarly journals Influence of propolis biologically active substances on blood biochemical parameters and morphometric indicators of intestines of store pigs

2020 ◽  
Vol 222 ◽  
pp. 02005
Author(s):  
N.V. Gaponov ◽  
G.L. Yagovenko ◽  
A.N. Stepanova ◽  
O.P Neverova ◽  
O.V. Gorelik

The results of research in some cases allow to judge the change in the functional activity of the studied organs after the use of the preparation. The obtained factual material significantly supplements the information about the biological properties of the propolis water-alcohol emulsion. In practical veterinary medicine, the results of research can be taken into account when prescribing propolis for therapeutic and preventive purposes. Thus, as a result of the study, it was found that the most characteristic histological changes in the structural components of the intestinal wall are observed in the ileum of young pigs and pigs of the experimental groups. The height of this intestine villi in young pigs of the experimental group is 280.97 ± 3.37 microns, and in the control group - 193.89 ± 2.66 microns (p< 0.001). In pigs treated with the preparation, the villi also have a higher height - 256.89 ± 1.62 microns compared to 163.96 ± 0.61 microns in the control group. In young pigs and pigs of the control groups, the shape of the ileum villi is finger-shaped or bottle-shaped, and in animals of the experimental groups their forms are more differentiated - bottle-shaped, leaf-shaped and dome-shaped. This will allow store pigs receiving propolis preparation to better absorb the nutrients of the diet and gain weight faster. The research results are of interest to researchers developing propolis preparations for oral use.

2021 ◽  
Vol 2021 (1) ◽  
pp. 55-64
Author(s):  
Nikolay Gaponov

This paper presents the results of experimental studies the influence of biologically active substances of propolis on micromorphometric parameters of the ileum in young pigs. The results of the experiment make it possible to ascertain a change in the functional activity of the studied organ after the use of the drug from propolis. Since the most active process of absorption of monomeric hydrolysis products, especially amino acids and carboxylic acids, occurs in the ileum. Hydrolysis of intermediate products to monomers and preparation of the transition to absorption is carried out by enzymes fixed on the intestinal cell membranes. And the level of bioconversion of feed and retention of nutrients depends on their shape and structure, which ultimately affects the payment for feed with products. Therefore, the study of the histology of the small intestine and especially the ileum is of great importance. So, as a result of the study, it was found that the most characteristic histological changes in the structural components of the intestinal wall are observed in young animals of the experimental groups. The thickness of the main lamina of the mucous membrane of the ileum in piglets in the control group is 308.28 ± 2.81 µm, in piglets in the experimental group – 255.49 ± 0.75 µm. The height of the villi of the ileum, in the animals of the control group, is significantly lower and amounts to 193.89 ± 2.66 µm versus 280.97 ± 3.37 µm in the experimental group. Similar changes are observed in young animals of the older age group. The height of intestinal villi in pigs of the experimental group significantly exceeds the indicator of the control group and is 256.89 ± 1.62 µm and 163.96 ± 0.61 µm, respectively. The muscle plate of the intestinal mucosa of the pigs in the experimental group is significantly thicker than the control and is 17.8 ± 0.2 μm and 8.89 ± 0.08 μm, respectively. The obtained experimental material substantially supplements the information on the biological properties of propolis preparations. In practical veterinary medicine, research results can be taken into account when prescribing propolis for medicinal and prophylactic purposes. Since histological analysis of intestinal sections did not reveal destructive or pathological changes in any of the compared groups. In feeding, using the obtained practical material, we can specify the stimulating effect of the propolis preparation to intensify the growth of animals depending on the age of the pigs.


2020 ◽  
Vol 2020 (4) ◽  
pp. 96-108
Author(s):  
Nikolay Gaponov

The research results in a number of cases allow us to judge about the change in the functional activity of the studied organs after the use of the drug. The obtained factual material significantly complements the information on the biological properties of the aqueous-alcoholic propolis emulsion. In practical veterinary medicine, research results can be taken into account when prescribing propolis for therapeutic and prophylactic purposes, since histological analysis of intestinal sections did not reveal destructive or pathological changes in all compared groups. In the field of zootechnics, the obtained practical material makes it possible to concretize the stimulating effect of the preparation in terms of stimulating the growth of animals depending on the age of pigs. So, as a result of the study, it was found that the most characteristic histological changes in the structural components of the intestinal wall are observed in the ileum of piglets and pigs of the experimental groups. The height of the villi of this intestine in piglets of the experimental piglet is 280.97 ± 3.37 μm, and in the control piglet – 193.89 ± 2.66 μm (p <0.001). In pigs receiving the drug, the villi also have a greater height – 256.89 ± 1.62 μm versus 163.96 ± 0.61 μm in the control group. In piglets and pigs of the control groups, the shape of the villi of the ileum is finger-shaped or bottle-shaped, while in the animals of the experimental groups their forms are more differentiated – bottle-shaped, leaf-shaped and dome-shaped. This will allow young animals receiving propolis to better absorb the nutrients of the diet and gain weight faster. The biochemical parameters of the blood of all animals in the dynamics of the experiment were within the physiological norm. The only exception was a slight decrease below the norm in piglets in the control group with reserve alkalinity of 44.81 ± 0.98 vol. % CO2, observed at the beginning of the experiment. The research results are of interest to researchers developing oral propolis preparations.


2019 ◽  
pp. 69-72
Author(s):  
Olga Nikolaevna Polozyuk ◽  
Tatyana Ivanovna Lapina

The authors found out that the application  of dietary supplements "Glimalask Lact" allowed preventing the disorder of the gastrointestinal tract and saving all piglets throughout the experiment.  After application of "Agrocid super oligo", the safety of purebred piglets was 100 %, and for two pedigree piglets it was 96.7%.   In piglets of the 1st and 2nd control groups, disorder of the gastrointestinal tract was noted during the completion of colostral immunity and at weaning from sows.  The safety of purebred piglets was 96.7%, and of the two pedigree piglets  it was 93.4%. The number of γg-globulins in the blood serum of two breed piglings treated with the Glimalask Lact supplement increased by 3.5 and 2.5% compared to the piglets of the experimental group fed with Agrocid super oligo, and of the control group, The number of g-globulins in the blood serum of purebred  piglets it increased by 2.4 and 3.1% compared with analogues of the experimental and control groups.


Author(s):  
I.V. Tsiganenko ◽  
L.K. Ovcharenko

The work considers the causes of the coronary X syndrome development in women by assessing the experimental group and the control group with typical angina pectoris with angiographically altered vessels. Each group included 30 patients. When studingy medical records of the patients in the study group, we found out that in the reproductive period all of them had hyperestrogenemia, confirmed by the laboratory data, with the corresponding consequences in the form of various gynecological diseases, while the patients of the control group had unburdened gynaecological history. In terms of the lipid spectrum, the results turned out to be opposite. In the experimental group, the rates were within the normal range, and the control level of LDL-C significantly exceeded the required values. Despite the fact that estrogens increase the concentration of high density lipoprotein (HDL) in the blood and lower the content of low density lipoprotein (LDL) that are atherogenic, their surplus has a less negative effect than their lack, as the risk of developing atherosclerosis increases with decreasing concentration, and with an increase there is a risk of developing endothelial dysfunction, which provokes the development of coronary syndrome X. These date confirm the development of endothelial dysfunction in the patients of the experimental group with hyperestrogenemia in the history resulted from the impairment of the process of proliferation of endothelial cells with subsequent imbalance of secretion of biologically active substances. Among them, there is nitric oxide, which causes the relaxation of smooth myocytes, thus resulting in vasodilatation, and endothelins, providing the opposite, vasodilating effect. Prostacyclines and thrombomodulins secreted by the vascular endothelium in physiological conditions, counteract platelet aggregation. In the case of damage to the vascular wall, the production of prostacyclin and thrombomodulin is suppressed, but the release of thromboplastin, platelet activation factor and von Willerband factor activates that promote platelet aggregation and blood clotting. Under the participation of other physiologically active substances, selectins, endothelial cells promote adhesion to their surface and further penetration into the site of inflammation of neutrophils, blood acidophils. Selectin is accumulated in the cytoplasm of the endothelial cells in the form of specific electron-cellular inclusions, the so-called bodies of Weibel-Palade. Normally, vascular endothelium is impervious to blood components. However, being affected by a number of factors, and in particular histamine, endothelial cells lose contact with each other and decrease in number. This leads to the release of water and plasma proteins into the intercellular medium causing oedema. Due to the ability of the inner layer of vessels to produce a large number of biologically active substances, such changes can hardly be corrected by therapy.


Author(s):  
А.И. Фролов ◽  
А.Н. Бетин

Жизненно важными микроэлементами для жвачных животных являются медь, цинк, марганец, железо, кобальт, йод, селен. Микроэлементы, в которых атом металла соединён и защищён белковыми молекулами, улучшают воспроизводительные качества самок за счёт повышения оплодотворяемости, уменьшения эмбриональной смертности, улучшения состояния мочеполовой системы или повышения интенсивности функционирования яичников. Поэтому в настоящее время актуальными являются разработка и внедрение в технологию кормления сельскохозяйственных животных наиболее эффективных препаратов с необходимыми биологически активными веществами. На основании проведённого исследования дано практическое обоснование использования в технологии кормления тёлок отечественной кормовой биодобавки на основе гидролизата соевого белка, витаминов и микроэлементов. Установлено её положительное действие на некоторые функции органов и систем животных. Экспериментально доказана эффективность её применения для оптимизации обменных процессов и повышения воспроизводительных функций тёлок. Так, биохимическим исследованием образцов крови подопытных животных установлено, что количество кальция и фосфора в крови у тёлок опытной группы по сравнению с контролем было достоверно выше на 2,35 и 2,9% соответственно. Эритропоэтическая фракция была более выражена у молодняка опытной группы. У тёлок опытной группы время от начала скармливания препарата до первой выраженной охоты и осеменения, по сравнению с контрольными животными, сократилось на 16%, а период от начала скармливания препарата до плодотворного осеменения – на 25,7%. Количество тёлок, пришедших в охоту и осеменённых в первый месяц, в опытной группе по сравнению с контрольной было на 43% больше. Оплодотворяемость тёлок опытной группы за учётный период составила 95%, что на 20% больше, чем у животных контрольной группы. Включение в рацион тёлок биодобавки в дозе 100 мл/гол. позволило получить дополнительно на 8,5% больше валового прироста живой массы по отношению к их аналогам. The vital trace nutrients for ruminants are copper, zinc, manganese, iron, cobalt, iodine, selenium. Microelements in which a metal atom is jointed and protected by protein molecules improve the reproductive qualities of females by increasing fertility, reducing embryonic mortality, improving the state of the genito-urinary system or increasing the intensity of ovarian functioning. Therefore the development and implementation of the most effective preparations with the necessary biologically active substances in the technology of feeding farm animals is currently relevant. On the ground of conducted research a practical substantiation of the use of domestic food supplement based on the hydrolyzate of soy protein, vitamins and trace nutrients in the feeding technology of heifers is given. Its positive effect on some functions of animal organs and systems has been established. The effectiveness of its application to optimize metabolic processes and increase the reproductive functions of heifers has been experimentally proven. Thus a biochemical blood samples analysis of experimental animals found that the amount of calcium and phosphorus in the blood of the heifers of the experimental group compared with the control was significantly higher by 2.35 and 2.9% respectively. The erythropoietic fraction was more defined in the young experimental group. In heifers of the experimental group the time from the beginning of feeding the drug to the first defined heat and insemination compared with control animals decreased by 16% and the period from the beginning of feeding the drug to prolific insemination – by 25.7%. The number of heifers that came to the heat and inseminated in the first month in the experimental group was 43% higher than the control group. The impregnation capacity of the heifers of the experimental group during the record period was 95% which is 20% more than in animals of the control group. Inclusion in the diet of heifers bioadditive at a dose of 100 ml / goal allowed obtaining additionally 8.5% more the gross body weight gain relative to their analogs.


Author(s):  
I. Ivanova ◽  
Yu. Karmatskikh

When balancing ration it is necessary to remember that the active factors of feeding are not the feed itself, but the content of nutrients and biologically active substances in them. It has been developed and physiologically justified feeding rations of cows during increasing the milk yield period, which are based on succulent feed, hay, grain and rapeseed presscake and a source of minerals and vitamins PVMA have been used. The experimental part of the work has been carried out in the winterstall period in the educational and experimental farm in the State Agrarian University of the Northern Trans-Urals. It has been found that from cows of the experimental group during the increasing the milk yield period 1503 kg of milk containing 3,98 % fat and 2,87 % protein has been obtained. Animals of the experimental group exceeded cows of the control group in milk yield by 90 kg (P > 0,95), in terms of fat and protein content in milk their superiority was 0,37 abs.% (P > 0,999) and 0,21 abs.% (P> 0,999). Input into the rations of lactating cows rapeseed presscake in the amount of 2 kg/ head/day and PVMA in the amount of 100 g during 3 months of lactation gives an increase in productivity for the entire lactation in the experimental group by 572 kg of milk with additional products by 2745,2 Rubles can be obtained more. The positive effect of the input of rapeseed presscake in the rations of lactating cows in the amount of 2 kg/head has been established/day and PVMA in the amount of 100 g in the period of following on the subsequent productivity of animals.


2020 ◽  
pp. 3-5
Author(s):  
Aleksandr I. Petenko ◽  
◽  
Nikita I. Petenko ◽  
Artem B. Vlasov ◽  
Natalya A. Yurina ◽  
...  

Aim of the research was to study the effect of premix with buffering properties in diets for newborn cows on their milk production, blood biochemical composition, microbiological parameters of scar fluid and its acidity. The experiment was carried out on 2 groups of calving black-motley cows, selected on the basis of the pair-analogue principle with 6 animals each. The first, control group of cows received a ration adopted on the farm. The second group of animals received the same diet, only instead of 0.5 kg of compound feed they used a multicomponent mineral premix enriched with biologically active additives, which has buffering properties, which is very important in the newborn period of cows. Feeding premix with buffering properties in the composition of the main diet to newborn cows contributed to an increase in the consumption of dry matter by animals. Since the cows of the second experimental group consumed more nutrients, a significant increase in the average daily milk yield was found. The studied premix helped establish a tendency to improve metabolic processes in the body of ruminants. In a scientific experiment, a positive effect of the buffer premix on the microbiological parameters of scar fluid, as well as its acidity and acid-binding ability, was established. The consumption of more nutrients was due to a significant increase in average daily milk yields by 9.1%. The results of biochemical studies of blood serum allow us to say that all indicators characterizing the metabolism were within the physiological norm in cows of both groups. Also, a qualitative change for the better in the microbiological parameters of scar fluid was noted. Feeding the studied premix to experienced animals contributed to the additional profit of 4069.8 rubles per head.


Author(s):  
А.В. МИШУРОВ

Проблема и цель. Для повышения эффективности использования питательных веществ кормов целесообразно применение биологически активных веществ (БАВ), способствующих улучшению физиологических процессов в организме животных для реализации их генетического потенциала – продуктивности. Целью исследований являлось изучить влияние БАВ (дигидроквертицина и органического йода) на рубцовый метаболизм у овец. Методология. Опыт проведен методом групп периодов в условиях физиологического двора ФНЦ ВИЖ имени Л.К. Эрнста, на овцах аналогах (n=6), имеющих хронические фистулы рубца по Басову. Животные 1-й опытной группы в дополнение к основному рациону получали 100 мл ДКВ, в рацион 2-й опытной группы добавляли 100 мг ДКВ и 1,05 мг органического йода. Результаты. Использование добавок в рационах животных опытных групп повлияло на общее содержание микроорганизмов в рубцовой жидкости, составившее во второй опытной группе 1,021 г/100 мл, что выше чем в первой опытной группе на 12,7 %, и выше, чем в контрольной, на 15,4 %. При этом образование бактерий в рубцовой жидкости в первой опытной группе было выше на 22,5 %, а во второй – на 8,7 % относительно контроля. Выявлен более низкий уровень концентрации аммиака в содержимом рубца животных первой опытной группы на 4,2 мг/% относительно контрольной и на 7,1 мг/% – второй опытной группы, что взаимосвязано с более эффективным его использованием рубцовой микрофлорой. Заключение. Полученные данные свидетельствуют о том, что использование в рационе изучаемых биологически активных веществ способствовало наибольшему развитию и жизнедеятельности микроорганизмов рубца и в целом способствовало регуляции положительной динамики рубцового метаболизма, оказало положительное влияние на образование и усвояемость азота. Problem and purpose. To increase the efficiency of the use of feed nutrients, it is advisable to use biologically active substances that contribute to the improvement of physiological processes in the body of animals for the realization of their genetic potential - productivity. The aim of the research was to study the effect of biologically active substances (dihydroquerticin and organic iodine) on rumen metabolism in sheep. Methodology. The experiment was carried out by the method of groups of periods in the conditions of the physiological yard of L.K. Ernst Federal Research Center for Animal Husbandry), on sheep analogs (n=6) with chronic rumen fistulas according to Basov. Animals of the 1st experimental group received 100 ml of DHQ in addition to the main diet. The 2nd experimental group got 100 mg of DHQ and 1.05 mg of organic iodine. Results. The use of additives in the diets of the animals of the experimental groups influenced the total content of microorganisms in the rumen fluid, which was 1.021 g / 100 ml in the second experimental group, that was higher than in the first experimental group by 12.7 %, and in the control group by 15.4 %. At the same time, the formation of bacteria in the rumen fluid in the 1st experimental group was 22.5 % higher and it was 8.7 % higher in the 2nd group relative to the control. A lower level of ammonia concentration in the rumen of animals of the 1st experimental group was 4.2 mg/% lower relative to the control and by 7.1 mg/% in the 2nd experimental group, that was interrelated with its more effective use of rumen microflora. Conclusion. The data obtained indicated that the use of the studied biologically active substances in the diet promoted the greatest development and vital activity of rumen microorganisms and, in general, contributed to the regulation of the positive dynamics of rumen metabolism, had a positive effect on the formation and assimilation of nitrogen.


Author(s):  
L. Yarmots ◽  
G. Yarmots ◽  
A. Belenkaya ◽  
M. Smyshlyaeva

Numerous studies have shown that sapropel is an effective source of macro-and microelements, as well as biologically active substances. There are about 3 million lakes on the territory of Russia, where sapropel reserves are estimated at 250 billion tons. There are 497 lakes on the territory of the Tyumen region, the estimated reserves of sapropel in them are 1398,7 million tons. The Tyumen region is a biogeochemical province for all normalized trace elements. Biologically active compounds obtained from natural raw materials, including normalized trace elements in organic form are the most promising and safe. Under the inviroments of the Tyumen region, such natural raw materials can be sapropels. The study of the effectiveness of the use of sapropels and their influence on the metabolism and milk productivity of cattle is an urgent topic. The purpose of the research was to study the influence of sapropel lake Nepryak on milk productivity indicators and the biochemical composition of the blood of cows. For the experiment 3 groups of cows have been formed (a control group and two experimental ones) based on the principle of pairs of analogs. Cows of the 1st experimental group have been got in the ration of 300 g of sapropel per head/day, 2nd experimental – 500 g/head/day. Cows of the control group did not receive sapropel in the ration. It has been found as the result of research that the use of sapropel lake Nepryak in feeding of cows in doses of 300 and 500 g/head/day had the positive influence on their milk productivity. At the same time, it is most effective to feed sapropel at the dose of 300 g/ head/day.


2021 ◽  
Vol 2 (4) ◽  
pp. 111-115
Author(s):  
Igor PETCU ◽  
Nicolae STARCIUC

Introduction. Currently, various biologically active substances, preferably of biological and not synthetic origin, with having a growth stimulating effect, are frequently used as food additives in the poultry industry. Some of these substances with a stimulating effect on the growth of chickens are represented by the biomass obtained from apathogenic strains of Streptomycetes. Material and methods. The investigations were conduvted within the ILR “Avicola Sărătenii-Vech” v. Sărătenii Vechi, Telenești district. The biological material was one-day-old Adler's silver chickens. The research was carried out on 150 chickens (one control and two experimental), which were randomly distributed in three groups of 50 chickens in each. Results. The dynamics of body weight was higher in the experimental group of chickens which, received biomass of Streptomycetes, indicating that the chickens in experimental group II recorded a 5.1% increase in body weight compared to chickens from the control group. The analysis of blood indices showed a slight increase of the number of lymphocytes and eosinophils, from 4 to 9% in experimental group II.  Conclusions. The use of biomass of Streptomycetes in feeding the experimental grup of chickens demonstrated an increase of body weight with 5.1% compared to the chickens from the control group. The biomass of Streptomycetes stimulated an increase of the number of limbocytes and eosinophils in chickens from the experimental group II, thus demonstrating an immunostimulatory effect.


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