scholarly journals Identification of 19-bp indel of the Pleomorphic Adenoma Gene 1 in Bali cattle population

2022 ◽  
Vol 335 ◽  
pp. 00011
Author(s):  
Sukaryo Sukaryo ◽  
Rizka Augustin ◽  
Yuli Yanti ◽  
Joko Riyanto ◽  
Slamet Diah Volkandari ◽  
...  
Keyword(s):  
Body Size ◽  
Pleomorphic Adenoma ◽  
Population Based ◽  
Potential Candidate ◽  
Cattle Population ◽  
Blood Samples ◽  
Reproduction Traits ◽  
Pcr Products ◽  
Bali Cattle ◽  
Dna Pool

Pleomorphic adenoma gene 1 (PLAG1) is a zinc finger transcription factor gene located on bovine chromosome 14 (BTA14) affecting body size and reproduction traits in cattle. The objective of this study was to identify 19-bp indel of the PLAG1 gene in Bali cattle population. A total of 96 blood samples of Bali Cattle were collected from Balai Pembibitan Ternak Unggul dan Hijauan Pakan Ternak (BPTU-HPT) Denpasar. Genomic DNA was extracted from blood samples and used to detect 19-bp indel of the PLAG1 gene using following primer pair 5’-TCCGAACAACAGGTGAGGGAGAAAT-3’ and 5’-CCACTTCAGG-GGTGCTCTAGGTTTG-3’. The polymerase chain reaction (PCR) products using DNA pool samples were sequenced to validate the PCR product and to find out novel polymorphism in Bali cattle population. The result showed that there was no variation found in Bali cattle population based on 19-bp indel of the PLAG1 gene, which is indicated by 123 bp DNA band. However, sequence analysis of the PLAG1 gene resulted in a novel single nucleotide polymorphism (SNP) at nucleotide number 32235 of the PLAG1 gene that changed guanine (G) to adenine (A). This novel SNP could be furthermore genotyped and it might be a potential candidate marker for body size and reproduction traits in Bali cattle.

Policy for increasing feeder stock of Bali cattle (Bos javonicus domesticus) in Kupang District

2020 ◽  
Vol 5 (6) ◽  
pp. 235-248
Author(s):  
Maria Krova ◽  
◽  
M. F. Lalus ◽  
Markus M. Kleden ◽  
◽  
...  
Keyword(s):  
Systems Approach ◽  
Population Based ◽  
Supply Management ◽  
Stock Management ◽  
Contributing Factors ◽  
Policy Interventions ◽  
Innovation And Technology ◽  
Calving Rate ◽  
Bali Cattle ◽  
The Difference

The scarcity of feeder stock of Bali cattle (Bos javonicus domesticus) in Kupang Regency is currently increasing. One of the contributing factors is the difference in management between the fattening business that produces beef cattle and the breeding business that produces feeders and heifers. The research aims to determine: the interaction between actors to increase the supply of feeder; the behavior of feeder population based on actual stock management, and the necessary policy interventions to increase the supply of feeder. The research was conducted by applying a dynamics systems approach. This modelling used ventana systems software. Data and mental models were collected through observation, focus group discussions, and in-depth interviews with key informants. The results showed that the interaction between actors was limited to the marketing of cattle for both local and domestic markets. The actual feeders supply management shows that cattle population tends to decline due to low calving rate (70%), high calves mortality (35%), and the high slaughtering of productive females (80%). The necessary policy interventions are to implement various efforts to increase the calving rate to be 80%, reduce the mortality rate by applying 2% feed supplementation, and reduce the sales of productive females to be 50%. The actual supply management of Bali feeders needs to be engineered by increasing stakeholder services that coordinate synergistically for learning innovation and technology. It is necessary to establish a breeder cooperative to suppress the sale of productive cows as the cause of its high slaughtering

Abstract P247: Ethnic Differences in Infrarenal Aortic Diameter and Area in a Population-Based Study

Circulation ◽  
2012 ◽  
Vol 125 (suppl_10) ◽  
Author(s):  
David E Timaran ◽  
Eric B Rosero ◽  
Adriana J Higuera ◽  
Ron Peshock ◽  
R James Valentine ◽  
...  
Keyword(s):  
Body Size ◽  
Ethnic Differences ◽  
Population Based ◽  
Aortic Diameter ◽  
Hispanic Ethnicity ◽  
Female Sex ◽  
Abdominal Aortic ◽  
Aortic Area ◽  
Blacks And Whites ◽  
Lower Height

Objective: Abdominal aortic aneurysms are defined as a 50% or greater increase in infrarenal aortic diameter (IAD). However, normal IAD has not been defined for all ethnic groups as minorities have been underrepresented in most studies. The aim of the study was to assess ethnic differences in IAD and infrarenal aortic area (AoA) adjusting for the effects of age, gender and body size in the general population. Methods: Participants (2,515) in a population based study underwent high-resolution magnetic resonance imaging (MRI) of the abdominal aorta. Analyses of variance and multiple regression analyses were used to assess the relationship between race/ethnicity, age, gender and body size and IAD and aortic area. Subjects with AAA detected by MRI (defined as IAD ≥ 3.0 cm) were excluded from the analysis. Results: Decreasing age, female sex, Hispanic ethnicity, and lower height were independent predictors of reduced IAD by multivariate linear regression (all P < 0.001). Of these, female sex and Hispanic ethnicity were the factors more strongly associated with aortic size. Female sex was associated with 0.27 cm reduction in IAD and Hispanic ethnicity with 0.39 cm reduction in IAD. Similarly, decreasing age, female sex, Hispanic ethnicity, and lower height were independent predictors of reduced AoA. Female sex was associated with a 51 cm 2 reduction in AoA and Hispanic ethnicity with 11.1 cm 2 reduction in AoA. Although Hispanics had higher BMI than blacks and whites ( P =.01), and lower height values than blacks and whites (P<.0001), IADs and AoAs were consistently lower among Hispanics. Conclusions: Ethnic differences exist in infrarenal aortic diameter. Despite larger body size, Hispanics have significantly lower IAD than blacks and whites in the general population. The reduced aortic size in Hispanics suggests that the thresholds for abdominal aortic aneurysm diagnosis, rupture and repair may be lower and need to be established.

scholarly journals SARS-CoV-2 Infection-Induced Promoter Hypomethylation as an Epigenetic Modulator of Heat Shock Protein A1L (HSPA1L) Gene

2021 ◽  
Vol 12 ◽  
Author(s):  
Jibran Sualeh Muhammad ◽  
Narjes Saheb Sharif-Askari ◽  
Zheng-Guo Cui ◽  
Mawieh Hamad ◽  
Rabih Halwani
Keyword(s):  
Epithelial Cells ◽  
Mrna Expression ◽  
Candidate Genes ◽  
Epigenetic Regulation ◽  
Dna Methyltransferases ◽  
Lung Epithelial Cells ◽  
Potential Candidate ◽  
Blood Samples ◽  
Lung Epithelial

Numerous researches have focused on the genetic variations affecting SARS-CoV-2 infection, whereas the epigenetic effects are inadequately described. In this report, for the first time, we have identified potential candidate genes that might be regulated via SARS-CoV-2 induced DNA methylation changes in COVID-19 infection. At first, in silico transcriptomic data of COVID-19 lung autopsies were used to identify the top differentially expressed genes containing CpG Islands in their promoter region. Similar gene regulations were also observed in an in vitro model of SARS-CoV-2 infected lung epithelial cells (NHBE and A549). SARS-CoV-2 infection significantly decreased the levels of DNA methyltransferases (DNMT1, DNMT3A, and DNMT3B) in lung epithelial cells. Out of 14 candidate genes identified, the expression of 12 genes was upregulated suggesting promoter hypomethylation, while only two genes were downregulated suggesting promoter hypermethylation in COVID-19. Among those 12 upregulated genes, only HSPA1L and ULBP2 were found to be upregulated in AZA-treated lung epithelial cells and immune cells, suggesting their epigenetic regulation. To confirm the hypomethylation of these two genes during SARS-CoV-2 infection, their promoter methylation and mRNA expression levels were determined in the genomic DNA/RNA obtained from whole blood samples of asymptomatic, severe COVID-19 patients and equally matched healthy controls. The methylation level of HSPA1L was significantly decreased and the mRNA expression was increased in both asymptomatic and severe COVID-19 blood samples suggesting its epigenetic regulation by SARS-CoV-2 infection. Functionally, HSPA1L is known to facilitate host viral replication and has been proposed as a potential target for antiviral prophylaxis and treatment.

scholarly journals GENOME-WIDE ASSOCIATION STUDY FOR MILK PRODUCTION AND REPRODUCTION TRAITS IN RUSSIAN HOLSTEIN CATTLE POPULATION

2016 ◽  
Vol 51 (2) ◽  
pp. 182-193 ◽  
Author(s):  
A.A. Sermyagin A.A. Sermyagin ◽  
◽  
E.A. Gladyr’ E.A. Gladyr’ ◽  
S.N. Kharitonov S.N. Kharitonov ◽  
A.N. Ermilov A.N. Ermilov ◽  
...  
Keyword(s):  
Association Study ◽  
Milk Production ◽  
Genome Wide Association Study ◽  
Genome Wide Association ◽  
Holstein Cattle ◽  
Cattle Population ◽  
Genome Wide ◽  
Reproduction Traits

Body size and the risk of multiple sclerosis in Norway and Italy: The EnvIMS study

2014 ◽  
Vol 21 (4) ◽  
pp. 388-395 ◽  
Author(s):  
Kristin Wesnes ◽  
Trond Riise ◽  
Ilaria Casetta ◽  
Jelena Drulovic ◽  
Enrico Granieri ◽  
...  
Keyword(s):  
Multiple Sclerosis ◽  
Risk Factor ◽  
Body Size ◽  
Case Control Study ◽  
Disease Onset ◽  
Large Body ◽  
Population Based ◽  
The Body ◽  
Outdoor Activity ◽  
Control Study

Background: Obesity may be a risk factor for developing multiple sclerosis (MS). Objective: We examined if body size influences the risk of MS in a population-based, case control study. Methods: A total of 953 cases and 1717 controls from Norway and 707 cases and 1333 controls from Italy reported their body size by choosing a silhouette 1 to 9 (largest) every fifth year from age 5 to 30 and at time of study. The body size-related MS risk was defined by odds ratios (ORs) in logistic regression analyses adjusting for age, smoking and outdoor activity. Results: In Norway a large body size (silhouettes 6–9) compared to silhouette 3 increased the risk of MS, especially at age 25 (OR 2.21; 95% CI 1.09–4.46 for men and OR 1.43; 95% CI 0.90–2.27 for women). When comparing silhouette 9 to 1, we found a significant dose-response from age 10 until age 30 peaking at age 25 (sex-adjusted OR 2.83; 95% CI 1.68–4.78). The association was present for at least 15 years prior to disease onset. No significant associations were found in Italy. Conclusions: Obesity from childhood until young adulthood is a likely risk factor for MS with a seemingly stronger effect in Norway than in Italy.

Mapping genes for growth rate and fatness in a Large White x Meishan F2 pig population

1998 ◽  
Vol 1998 ◽  
pp. 7-7 ◽  
Author(s):  
G.A. Walling ◽  
A.L Archibald ◽  
P.M. Visscher ◽  
C.S. Haley
Keyword(s):  
Growth Rate ◽  
Genetic Variation ◽  
Experimental Design ◽  
Statistical Methods ◽  
Population Based ◽  
Genetic Maps ◽  
Large White ◽  
Backcross Population ◽  
Livestock Species ◽  
Reproduction Traits

DNA-based markers and genetic maps of major livestock species have been developed in the last few years. These, together with experimental populations and appropriate statistical methods, provide the tools to dissect causes of economically important genetic variation in livestock. A cross between genetically diverse lines is a powerful experimental design for such a study. In an F2 or backcross population from such a cross, markers are likely to be highly informative and the genes that control the differences between the lines will be segregating. We have been developing such a resource population based on a cross between the British Large White and Chinese Meishan pigs. These breeds differ for many traits, with the Meishan being inferior for growth rate and fatness, but superior for reproduction traits. Here we report the mapping of major genetic effects on growth rate and fatness in our cross.

scholarly journals Expression Analysis of the PITX2 Gene and Associations between Its Polymorphisms and Body Size and Carcass Traits in Chickens

Animals ◽  
2019 ◽  
Vol 9 (12) ◽  
pp. 1001 ◽  
Author(s):  
Haiyue Cao ◽  
Xinyang Dong ◽  
Haiguang Mao ◽  
Ningying Xu ◽  
Zhaozheng Yin
Keyword(s):  
Body Size ◽  
Expression Analysis ◽  
Myogenic Differentiation ◽  
Carcass Traits ◽  
The Body ◽  
Expression Level ◽  
Potential Candidate ◽  
Pitx2 Gene ◽  
Leg Muscle ◽  
Wuliang Mountain

PITX2 is expressed in and plays an important role in myocytes of mice, and it has effects on late myogenic differentiation in chickens. However, the expression profile and polymorphisms of PITX2 remain unclear in chickens. Therefore, the aim of the present study was to detect its expression and investigate single nucleotide polymorphisms (SNPs) within its exons and then to evaluate whether these polymorphisms affect body size as well as carcass traits in chickens. The expression analysis showed that the expression level of chicken PITX2 mRNA in the leg muscle and hypophysis was significantly higher (p < 0.01) than those in other tissues. The results of polymorphisms analysis identified two SNPs (i.e., g.9830C > T and g.10073C > T) in exon 1 and 10 SNPs (i.e., g.12713C > T, g.12755C > T, g.12938G > A, g. 3164C > T, g.13019G > A, g.13079G > A, g.13285G > A, g.13335G > A, g.13726A > G and g.13856C > T) in exon 3, including four novel SNPs (i.e., g.9830C > T, g.12713C > T, g.12938G > A and g.13856C > T). In the loci of g.10073C > T and g.12713C > T, chickens with the CT genotype had the highest (p < 0.05 or p < 0.01) breast depth and breast angle, respectively. For the locus of g.13335G > A, chickens with the GG genotype had the highest (p < 0.05 or p < 0.01) breast angle and shank circumference. For the locus of g.13726A > G, chickens with the GG genotype had the highest breast width, fossil keel bone length and shank circumference. The locus of g.12713A > G had significant effects on the PITX2 mRNA expression level in leg muscle. The H1H7 diplotype showed the highest shank circumference, and the H2H8 diplotype showed the highest breast muscle rate. The present research suggested that polymorphisms of the exons of the PITX2 gene were significantly associated with the body size and carcass traits of Wuliang Mountain Black-bone chickens and the PITX2 gene could be a potential candidate gene for molecular marker-aided selection in Wuliang Mountain Black-bone chickens and other chicken breeds.

scholarly journals Low Serum IGF-1 in Boys with Recent Onset of Juvenile Idiopathic Arthritis

2018 ◽  
Vol 2018 ◽  
pp. 1-10
Author(s):  
Anna-Carin Lundell ◽  
Malin Erlandsson ◽  
Maria Bokarewa ◽  
Hille Liivamägi ◽  
Karin Uibo ◽  
...  
Keyword(s):  
Juvenile Idiopathic Arthritis ◽  
Disease Duration ◽  
Population Based ◽  
Study Group ◽  
Newly Diagnosed ◽  
Biological Mechanisms ◽  
Blood Samples ◽  
Recent Onset ◽  
Estonian Population ◽  
Age And Sex

Background. Liver-derived insulin-like growth factor-1 (IGF-1) contributes bone formation. Decreased IGF-1 levels are common in juvenile idiopathic arthritis (JIA), but whether IGF-1 is related to sex and differ during the pathogenic progress of JIA is unknown. Objective. The aim of this study was to examine IGF-1 levels in boys and girls with newly diagnosed JIA, with established JIA and in controls. Methods. The study group included 131 patients from the Estonian population-based prevalence JIA study. Blood samples were obtained from 27 boys and 38 girls with early JIA (≤1 month from the diagnosis), 29 boys and 36 girls with established JIA (mean disease duration 18 months), and from 47 age- and sex-matched controls. Results. IGF-1 levels in boys were significantly decreased in early JIA compared to male controls, while IGF-1 levels in girls were comparable between JIA and controls. In early JIA, IGF-1 levels were 12-fold lower in boys relative to girls. In controls, IGF-1 levels correlated with both age and height, while these correlations were lost in boys with early JIA. Conclusion. We report a sex-dependent deficiency in serum IGF-1 in boys with early JIA, which argues for sex-related differences in biological mechanisms involved in the disease pathogenesis.

scholarly journals Suicidal ideation in relation to disordered eating, body size and weight perception: a cross-sectional study of a Norwegian adolescent population: the HUNT Study

BMJ Open ◽  
2019 ◽  
Vol 9 (7) ◽  
pp. e029809 ◽  
Author(s):  
Farzaneh Saeedzadeh Sardahaee ◽  
Turid Lingaas Holmen ◽  
Nadia Micali ◽  
Erik R Sund ◽  
Ottar Bjerkeset ◽  
...  
Keyword(s):  
Body Size ◽  
Suicidal Ideation ◽  
Disordered Eating ◽  
Weight Perception ◽  
Population Based ◽  
Cross Sectional Study ◽  
Cross Sectional ◽  
Logistic Regression Models ◽  
Norwegian Population ◽  
Hunt Study

ObjectiveWe conducted a population-based study on a sample of more than 7000 adolescents where we examined the associations between suicidal ideation (SI) and disordered eating (DE) and its related traits.DesignCross-sectional.SettingsData were derived from two Norwegian population-based cohorts, the Young-HUNT1 (1995–1997) and Young-HUNT3 (2006–2008) from the county of Nord-Trøndelag, Norway.ParticipantsA total of 7268 adolescents (15–19 years) who had completed self-reported questionnaires including items on SI, DE, body size and weight perception were included.Primary outcome measuresORs for SI given DE, body size or weight perception. Analyses were performed in multivariate logistic regression models.ResultsThe prevalence of SI was 23.1% in total population. Both girls and boys who reported DE, evaluated their body size asnot‘about the same as others’ or were ‘unhappy about their weight’ had between twofold to fivefold increase in odds for SI; these incremental risks were observed independent of sex, age, body mass index and socioeconomic status. We observed higher odds for SI among boys.ConclusionsOur findings suggest a clear association between SI and DE and its associated traits, in both genders but especially in males. Special attention should be paid on early detection of DE traits among adolescents.

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