Identification of glyphosate-resistant Lolium rigidum and Raphanus raphanistrum populations within the first Western Australian plantings of transgenic glyphosate-resistant canola

2015 ◽  
Vol 66 (9) ◽  
pp. 930
Author(s):  
Michael B. Ashworth ◽  
Michael J. Walsh ◽  
Ken C. Flower ◽  
Stephen B. Powles

Transgenic glyphosate-resistant canola was first commercially grown in Western Australia (WA) in 2010, providing an opportunity to obtain important baseline data regarding the level of glyphosate resistance in weeds following the exclusive use of glyphosate for in-crop weed control. In this study, two surveys (2010 and 2011) were conducted across the 14 Mha of the grainbelt of WA. The 2010 survey was carried out at the late-flowering stage of glyphosate-resistant canola, whereas the 2011 survey was conducted at an earlier growth stage (6–8 leaves), ~2–3 weeks after the second in-crop glyphosate application. During the surveys, 239 fields were visited, representing an estimated combined area of 24 000 ha. The 2011 survey alone represented a subsample of 23% of the total glyphosate-resistant canola planting in the WA grainbelt for that season. Glyphosate resistance was identified in one population of wild radish (Raphanus raphanistrum L.) and in eight annual ryegrass (Lolium rigidum L.) populations. None of the tested capeweed (Arctotheca calendula (L.) Levyns) populations were glyphosate-resistant. In this survey, no populations of barley grass (Hordeum spp.), brome grass (Bromus spp.), wild oat (Avena spp.) or small-flowered mallow (Malva parviflora L.) survived glyphosate application. Despite a long history of pre-seeding and fallow glyphosate use in WA, this survey found that glyphosate still provides excellent in-crop control of most species; however, some resistance is evident, requiring diverse weed control techniques to limit their spread.

2004 ◽  
Vol 44 (12) ◽  
pp. 1195 ◽  
Author(s):  
M. Monjardino ◽  
D. J. Pannell ◽  
S. B. Powles

Most cropping farms in Western Australia must deal with the management of herbicide-resistant populations of weeds such as annual ryegrass (Lolium rigidum) and wild radish (Raphanus raphanistrum). Farmers are approaching the problem of herbicide resistance by adopting integrated weed management systems, which allow weed control with a range of different techniques. These systems include non-herbicide methods ranging from delayed seeding and high crop seeding rates to the use of non-cropping phases in the rotation. In this paper, the Multi-species RIM (resistance and integrated management) model was used to investigate the value of including non-cropping phases in the crop rotation. Non-crop options investigated here were haying and green manuring. Despite them providing excellent weed control, it was found that inclusion of these non-cropping phases did not increase returns, except in cases of extreme weed numbers and high levels of herbicide resistance.


1950 ◽  
Vol 40 (3) ◽  
pp. 263-274 ◽  
Author(s):  
H. A. Roberts ◽  
G. E. Blackman

During 1947–9, field and pot experiments have been carried out first to assess the herbicidal properties of 2:4-dinitro-6-secondary-butyl-phenol, and secondly to determine its value for selective weed control in leguminous crops. In order to obtain precise estimates of the concentration required to produce a given percentage mortality of either weed or crops species, such experiments were so designed that the results are capable of probit analysis.From the data of field experiments on thirteen species of annual weeds, it is evident that there are wide variations in the concentration required to produce a standard kill. Irrespective of the species, susceptibility is greatest in the young seedling stage and there is a rapid increase in resistance with age. For example, with Raphanus raphanistrum between the cotyledon and pre-flowering stage a sixfold increase in concentration is needed to give a 90% mortality.


Plants ◽  
2021 ◽  
Vol 10 (9) ◽  
pp. 1879
Author(s):  
Martina Badano Perez ◽  
Hugh J. Beckie ◽  
Gregory R. Cawthray ◽  
Danica E. Goggin ◽  
Roberto Busi

Overreliance on herbicides for weed control is conducive to the evolution of herbicide resistance. Lolium rigidum (annual ryegrass) is a species that is prone to evolve resistance to a wide range of herbicide modes of action. Rapid detection of herbicide-resistant weed populations in the field can aid farmers to optimize the use of effective herbicides for their control. The feasibility and utility of a rapid 7-d agar-based assay to reliably detect L. rigidum resistant to key pre- and post-emergence herbicides including clethodim, glyphosate, pyroxasulfone and trifluralin were investigated in three phases: correlation with traditional pot-based dose-response assays, effect of seed dormancy, and stability of herbicides in agar. Easy-to-interpret results were obtained using non-dormant seeds from susceptible and resistant populations, and resistance was detected similarly as pot-based assays. However, the test is not suitable for trifluralin because of instability in agar as measured over a 10-d period, as well as freshly-harvested seeds due to primary dormancy. This study demonstrates the utility of a portable and rapid assay that allows for on-farm testing of clethodim, glyphosate, and pyroxasulfone resistance in L. rigidum, thereby aiding the identification and implementation of effective herbicide control options.


Weed Science ◽  
2012 ◽  
Vol 60 (3) ◽  
pp. 474-479 ◽  
Author(s):  
Yazid Bostamam ◽  
Jenna M. Malone ◽  
Fleur C. Dolman ◽  
Peter Boutsalis ◽  
Christopher Preston

Glyphosate is widely used for weed control in the grape growing industry in southern Australia. The intensive use of glyphosate in this industry has resulted in the evolution of glyphosate resistance in rigid ryegrass. Two populations of rigid ryegrass from vineyards, SLR80 and SLR88, had 6- to 11-fold resistance to glyphosate in dose-response studies. These resistance levels were higher than two previously well-characterized glyphosate-resistant populations of rigid ryegrass (SLR77 and NLR70), containing a modified target site or reduced translocation, respectively. Populations SLR80 and SLR88 accumulated less glyphosate, 12 and 17% of absorbed glyphosate, in the shoot in the resistant populations compared with 26% in the susceptible population. In addition, a mutation within the target enzyme 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) where Pro106had been substituted by either serine or threonine was identified. These two populations are more highly resistant to glyphosate as a consequence of expressing two different resistance mechanisms concurrently.


2004 ◽  
Vol 55 (10) ◽  
pp. 1047 ◽  
Author(s):  
Kathryn J. Steadman ◽  
Amanda J. Ellery ◽  
Ross Chapman ◽  
Andrew Moore ◽  
Neil C. Turner

The role of temperature and rainfall during seed development in modulating subsequent seed dormancy status was studied for Lolium rigidum Gaud. (annual ryegrass). Climatic parameters relating to geographic origin were compared with annual ryegrass seed dormancy characteristics for seeds collected from 12 sites across the southern Western Australian cropping region. Seed germination was tested soon after collection and periodically during subsequent after-ripening. Temperature in the year of seed development and long-term rainfall patterns showed correlations with aspects of seed dormancy, particularly the proportion of seeds remaining dormant following 5 months of after-ripening. Consequently, for one population the temperature (warm/cool) and water supply (adequate/reduced) during seed development were manipulated to investigate the role of maternal environment in the quantity and dormancy characteristics of seeds produced. Seeds from plants grown at warm temperatures were fewer in number, weighed less, and were less dormant than those from plants grown at cool temperature. Seeds that developed under both cool temperature and reduced moisture conditions lost dormancy faster than seeds from well-watered plants. Seed maturation environment, particularly temperature, can have a significant effect on annual ryegrass seed numbers and seed dormancy characteristics.


2021 ◽  
Vol 2 ◽  
Author(s):  
Guy Coleman ◽  
Christopher Betters ◽  
Caleb Squires ◽  
Sergio Leon-Saval ◽  
Michael Walsh

Increasing concern for the ongoing availability and efficacy of herbicides is driving interest in the development of alternative physical and thermal weed control methods. Fortunately, improvements in weed detection through advancements in computing hardware and deep learning algorithms are creating an opportunity to use novel weed control tools, such as lasers, in large-scale cropping systems. For alternative control options, there are two key weed control timing opportunities, early and late post-crop emergence. Weed density for the early timing is typically higher, with a shorter window for control. Conversely, late post-emergent treatment of surviving and late-emerging weeds would occur in lower densities of larger and more variably sized weeds, given a prior weed control effort, but with a longer available weed control period. Research in laser weeding to date has primarily focused on early growth stage weeds and the ability of this approach to control larger weeds remains unknown. This study used a 25 W, 975 nm fiber-coupled diode laser to evaluate the opportunity for control of annual ryegrass (Lolium rigidum Gaudin) and the influence of four different growth stages (three-leaf, seven-leaf, mid-tillering, and late-tillering). Annual ryegrass plants at each growth stage were treated using a laser-focused to a 5 mm diameter with five different irradiation durations developing energy densities of 1.3, 2.5, 6.4, 19.1, and 76.4 J mm−2. At the three-leaf stage, all plants were controlled at 76.4 J mm−2 and 93.3% controlled at 19.1 J mm−2. Complete control of seven-leaf plants was only achieved at 76.4 J mm−2. Although laser treatments did not control mid-tillering stage plants, 76.4 J mm−2 reduced biomass by 60.2%. No similar reductions in biomass were recorded for the largest plants. This initial research assists in the development of novel weed control options in the context of large-scale conservation cropping systems. Future research should investigate the influence of laser treatments on additional weed species and the impact of increased laser power on larger weeds.


2021 ◽  
Vol 17 (3) ◽  
pp. 70-82
Author(s):  
Washington Louis Franklin Termineles Zacharias ◽  
Pedro Henrique Caldeirão Pierro ◽  
Guilherme Mendes Pio Oliveira ◽  
Rubia Fernanda Bovo ◽  
Leonardo Giorgiani Zarelli ◽  
...  

The use of pre-emergent herbicides is an important tool in weedmanagement. Sulfentrazone + diuron mixture was recently launched ontothe market. The objectiveof this work was to evaluate the weed control and the agronomic performance of soybean in response to application of sulfentrazone + diurondoses in pre-emergence. The experiment was conducted in Londrina PR, in an oxisol. The treatments were doses ofsulfentrazone + diuron (62 + 123, 123 + 245, 184 + 368, 245 + 490, 306 + 613 e 368 + 735 g a.i.ha-1). In addition to these treatments, isolated applications of sulfentrazone (245 g a.i.ha-1) and diuron (490 g a.i.ha-1) were evaluated, as well as a weedyand aweed-freecontrol. For the weed control, the wild radish(Raphanus raphanistrum)and itchgrass (Rottboellia cochinchinensis) emergencewere evaluated. In soybean, photosystem II (PSII)activity, stand, plant height, number of podsper plant, height of insertion of the first pod, number of nodes per plant, mass of a thousand grains and grain yield were evaluated. Results show that the mixture of sulfentrazone + diuron resulted in less than 50% wild radish control, regardless of the dose evaluated. However, for itchgrass, the control was greater than 90% in doses from 123 g sulfentrazone + 245g diurona.i.ha-1. Regarding thesoybeancrop, in doses from 245 g sulfentrazone + 490 g diuron a.i. ha-1 there was a greater number of pods per plant and higher grain yield, of about3,000 kg ha-1. The application of herbicides led to a transient inhibition of PSIIactivity, which did not result in a reduction in crop grainyield, indicating plant recovery. Therefore, the mixture of sulfentrazone + diuron is a good alternative for the weed management in pre-emergence of soybean, and should be positioned according to the history of fieldinfestation.


Weed Science ◽  
2004 ◽  
Vol 52 (6) ◽  
pp. 968-975 ◽  
Author(s):  
Robert S. Gallagher ◽  
Kathryn J. Steadman ◽  
Andrew D. Crawford

The effect of hydration (priming) treatment on dormancy release in annual ryegrass seeds from two populations was investigated. Hydration duration, number, and timing with respect to after-ripening were compared in an experiment involving 15 treatment regimens for 12 wk. Seeds were hydrated at 100% relative humidity for 0, 2, or 10 d at Weeks 1, 6, or 12 of after-ripening. Dormancy status was assessed after each hydration treatment by measuring seed germination at 12-hourly alternating 25/15 C (light/dark) periods using seeds directly from the hydration treatment and seeds subjected to 4 d postpriming desiccation. Seeds exposed to one or more hydration events during the 12 wk were less dormant than seeds that remained dry throughout after-ripening. The longer hydration of 10 d promoted greater dormancy loss than either a 2-d hydration or no hydration. For the seed lot that was most dormant at the start of the experiment, two or three rather than one hydration event or a hydration event earlier rather than later during after-ripening promoted greater dormancy release. These effects were not significant for the less-dormant seed lot. For both seed lots, the effect of a single hydration for 2 d at Week 1 or 6 of after-ripening was not manifested until the test at Week 12 of the experiment, suggesting that the hydration events alter the rate of dormancy release during subsequent after-ripening. A hydrothermal priming time model, usually used for modeling the effect of priming on germination rate of nondormant seeds, was successfully applied to dormancy release resulting from the hydration treatments.


2002 ◽  
Vol 42 (2) ◽  
pp. 111 ◽  
Author(s):  
R. Stanton ◽  
J. Piltz ◽  
J. Pratley ◽  
A. Kaiser ◽  
D. Hudson ◽  
...  

A trial was conducted to investigate the survival and digestibility of annual ryegrass (ARG) seed (Lolium rigidum L.) eaten by sheep and cattle. Sheep (n= 8) and cattle (n = 8) were fed a basal diet containing 1:1 lucerne chaff:oaten chaff with (ARG) or without (control) the inclusion of 20% total dry matter of annual ryegrass seed in a changeover design. Intake was restricted to 17 g/kg liveweight. Digestibility of the control diet was lower (P<0.01) for sheep than cattle. Annual ryegrass seed was present (P<0.01) in the faeces of both sheep and cattle within 24 h of first ingestion. Some 10.8 and 32.8% of seed ingested was excreted by sheep and cattle respectively, with 3.9% (sheep) and 11.9% (cattle) remaining germinable. Annual ryegrass seed continued to be excreted by both sheep and cattle up to 5 days after removal from the diet. Dry matter digestibility of the annual ryegrass diet was 53% in cattle.


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