Volatile Secondary Metabolites from ColombianCroton malambo(Karst) by Different Extraction Methods and Repellent Activity of its Essential Oil

2014 ◽  
Vol 17 (5) ◽  
pp. 992-1001 ◽  
Author(s):  
Beatriz E. Jaramillo-Colorado ◽  
Muñoz C Karen ◽  
Duarte R Edisson ◽  
Stashenko Elena ◽  
Olivero V Jesus
2021 ◽  
Vol 6 (2) ◽  
Author(s):  
Modinat A. Adekoya ◽  
Nadrat J. Akeem

Normal oil and Essential oil from fresh leaves of Eucalyptus torelliana (F. Muell) were extracted using solvent (Soxhlet) and steam distillation extraction methods respectively. The leaves were screened for the presence of secondary metabolites and the extracted oils for their antimicrobial potentials. The phytochemical screening revealed the presence of Phytates (27.81 mg/g) Phytic acids (7.833mg/g) Oxalates (3.061mg/g) Tannins (2.457mg/g) Phenols (30.00%) Flavonoids (2.948%) Saponins (3.166%) and Alkaloids (2.84%). In addition to this the leaves were found to contain anthraquinone. The two oils extracted from the leaves of Eucalyptus torelliana were studied for their antimicrobial activity against the following isolated microorganisms: Xanthomonas axonopodis, Shigella dysenterine, Pseudomonas solanecearium, Streptococcus faecales, Staphylococcus aureus, Salmonella enterica, Erwinia carotovora, Salmonella typhi. The oils were found to inhibit all the microorganism isolates. The zone of inhibition exhibited by the extracts on the tested microorganism was between 22-6mm. The oil extracts compared favourably with Ampiclox used as a standard control.  The results obtained from this study reveals that the oils extracted from Eucalyptus torelliana has antibacterial activities against enteric pathogens and the oil may be potential source of new antimicrobials against enteric organisms.Keywords- Enteric microorganisms, essential oil, Eucalyptus torelliana, secondary metabolites, solvent extraction


2020 ◽  
Vol 75 (11) ◽  
pp. 1451-1460
Author(s):  
Z. A. Temerdashev ◽  
V. V. Milevskaya ◽  
L. P. Ryabokon’ ◽  
N. N. Latin ◽  
N. V. Kiseleva ◽  
...  

Molecules ◽  
2019 ◽  
Vol 24 (11) ◽  
pp. 2053 ◽  
Author(s):  
Yaoyao Peng ◽  
Karen Suzanne Bishop ◽  
Siew Young Quek

Feijoa is an aromatic fruit and the essential oil from feijoa peel could be a valuable by-product in the juicing industry. An initial comparison of the essential oil extraction methods, steam-distillation and hydro-distillation, was conducted. The volatile compounds in the essential oils from four feijoa cultivars were identified and semi-quantified by GC-MS and the aroma active compounds in each essential oil were characterized using SPME-GC-O-MS. Hydro-distillation, with a material to water ratio of 1:4 and an extraction time of 90 min, was the optimized extraction method for feijoa essential oil. The Wiki Tu cultivar produced the highest essential oil yield among the four selected cultivars. A total of 160 compounds were detected, among which 90 compounds were reported for the first time in feijoa essential oils. Terpenes and esters were dominant compounds in feijoa essential oil composition and were also major contributors to feijoa essential oil aroma. Key aroma active compounds in feijoa essential oils were α-terpineol, ethyl benzoate, (Z)-3-hexenyl hexanoate, linalool, (E)-geraniol, 2-undecanone, 3-octanone, α-cubebene, and germacrene D. This is the first report on the optimization of the extraction method and the establishment of the aroma profile of feijoa essential oils, with a comparison of four New Zealand grown cultivars.


Molecules ◽  
2021 ◽  
Vol 26 (10) ◽  
pp. 2949
Author(s):  
Juan I. Burneo ◽  
Ángel Benítez ◽  
James Calva ◽  
Pablo Velastegui ◽  
Vladimir Morocho

Chemical compositions of plants are affected by the initial nutrient contents in the soil and climatic conditions; thus, we analyzed for the first time the effects of soil and leaf nutrients on the compositions of the essential oils (EOs) of Siparuna muricata in four different localities in Ecuador. EOs were obtained by hydrodistillation and analyzed by gas chromatography/mass spectrometry (GC/MS) and a gas chromatography/flame ionization detector (GC/FID). Enantiomeric distribution by GC/MS was determined, modifying the enantiomeric separation of β-pinene, limonene, δ-elemene, β-bourbonene, cis-cadina-1 (6), 4-diene and atractylone. A total of 44 compounds were identified. The most representative for L1 were guaiol, atractylone and 4-diene; for L2, cis-cadina-1(6),4-diene and myrcene; for L3, atractylone, myrcene and germacrene B; and finally, L4 germacrene B, myrcene and cis-cadina-1(6),4-diene. Correlations between soil- leaf chemical elements such as Al, Ca, Fe, Mg, Mn, N and Si in the different localities were significant with chemical composition of the essential oil of Siparuna muricata; however, correlations between soil and leaf K, P, and Na were not significant. Cluster and NMDS analysis showed high dissimilarity values of secondary metabolites between four localities related with changes in soil- leaf nutrients. Thus, the SIMPER routine revealed that not all secondary metabolites contribute equally to establishing the differences in the four localities, and the largest contributions are due to differences in guaiol, cis-cadina-1(6),4-diene, atractylone and germacrene. Our investigation showed for the first time the influences of altitude and soil- leaf chemical elements in the chemical composition of the EOs of S. muricata.


2018 ◽  
Vol 19 (4) ◽  
Author(s):  
Tadeusz Wolski ◽  
Bogdan Kędzia

A cup plant (Silphium perfoliatum L.) is a poorly-known crop in the country. It came to Europe from North America in the 18th century. It can be propagated by dividing rhizomes or by sowing seeds. This plant is undemanding in terms of alimentary requirements and resistant to cold and pests. Due to the high content of nutrients and the yield of green mass a cup plant is used for fodder purposes. A cup plant contains numerous secondary metabolites, such as phenolic acids, flavonoids, terpenes, essential oil, tannins, vitamins and mineral components. It is characterized by antioxidant, antibacterial, antifungal, cytostatic and immunosuppressive activity. Research on laboratory animals indicates that the cup plant accelerates wound healing and indicate hypolipemic and estrogenic activity. Numerous reports confirm, that this plant also has a strengthening, diuretic, anti-inflammatory, antihemorrhagic and spasmolytic activity. In addition, it is used in the healing diseases of the liver and spleen, and also heals gastric and duodenal ulcers.


2015 ◽  
Vol 17 (4 suppl 1) ◽  
pp. 769-773 ◽  
Author(s):  
R.L. CANSIAN ◽  
V. ASTOLFI ◽  
R.I. CARDOSO ◽  
N. PAROUL ◽  
S.S. ROMAN ◽  
...  

ABSTRACT The aim of this work was to evaluate the insecticidal and repellency activity of the essential oil of Cinnamomum camphora var. linaloolifera Y. Fujita (Ho-Sho) and Cinnamomumcamphora (L.) J Presl.var. hosyo (Hon-Sho), against the Sitophilus zeamais in maize grains. The essential oils were obtained by hydrodistillation and analyzed by GC-MS.The insecticidal activity was determined by the toxicity of different concentrations of essential oils during 24 hours of contact with the insects, in the absence of feed substrate. The Bioassays of repellency were conducted with lethal doses (LD50,LD25,and LD12.5) obtained from insecticidal bioassay. In order to compare the treatments the preference index (PI) was employed. The analysis of the essential oils of Cinnamomum camphora leaves indicated 68% of camphor and 9% of linalool for the variation Hon-Sho and 95% of linalool to the variation Ho-Sho. The variation Ho-Sho presented greatest insecticidal activity than the variation Hon-Sho against the Sitophiluszeamais, with LD50 of 0.35 μL/cm2, whereas in the variation Hon-Sho the ratewas 0.48 μL/cm2. However, considering only the concentrations of linalool and camphor of Ho-Sho and Hon-Sho, the lethal doses’ evaluation of these compounds were similar. The values of the preference index ranged from -0.3 to -0.8 for thevariation Ho-Sho and -0.2 to -0.7 for the variation Hon-Sho. The essential oils evaluated in this work showed repellent activity against Sitophiluszeamais in vitro and in trials performed in mini-silos.


Author(s):  
Ayu Chandra Kartika Fitri ◽  
Fikka Kartika Widyastuti

One source of essential oil that has long been popular is a citrus fruit (Citrus aurantium). Results waste citrus peel around 500,000 tonnes per year. So the prospects for the utilization of waste orange peel are big enough to extract the essential oil. However, conventional extraction methods have drawbacks in terms of product quality, so to find solutions to these shortcomings, it is necessary to use the extraction method by Microwave Hydrodiffusion and Gravity (MHG). This method combines the use of microwave heating and physical phenomena (hydrodiffusi and earth gravity) at atmospheric pressure, without addition of organic solvents or water. In this study used fresh orange peel material 400 and 500 grams, two variable microwave power of 100 and 300 Watt and the extraction time for 15, 30, 45 and 60 minutes. To know the processing time and the microwave power effective method of MHG, oil composition was analyzed by GCMS to determine the amount of the fraction of oxygenated compounds. The larger of installed microwave power, the higher of operating temperature, so the %yield increases in a shorter time. From the comparison of energy consumed, the MHG method is give 30% more energy efficient than the Microwave Hydrodistillation (MHD) method. The use of microwaves does not change the physical properties of oil. The oil produced from the MHG method has a good quality because it has specific gravity and refractive index values that meet the EOA quality standard and has a greater oxygenated compounds content than the MHD method


2016 ◽  
Vol 3 (3) ◽  
pp. 312 ◽  
Author(s):  
Zsuzsanna Pluhár ◽  
Dóra Szabó ◽  
Szilvia Sárosi

Thymus vulgaris L. is a well-know medicinal and aromatic plant native to the Mediterranean region. The essential oil is considered as the main active constituent, being responsible for its typical odour and taste as well as for several therapeutic effects. Our aim was to demonstrate the most important factors influencing the quality and quantity parameters of thyme oil by summarizing the available literature data and our own scientific results. Genetic background, climatic and growing conditions, techniques of primary processing, storage conditions as well as different extraction methods have proven effects on the essential oil properties and, as a consequence, on its biological activity, either.


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