Correlation Phenolic Concentration to Antioxidant and Antibacterial Activities of Several Ethanolic extracts from Indonesia

Betel leaf ethanolic extract dechlorophyllized using sedimentation (BLEE-SED) exhibited enhanced antioxidant and antibacterial activities. BLEE-SED could extend the shelf-life of Nile tilapia fillets during refrigerated storage (4 °C) up to 9 days.

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Antibacterial Activities of the Aqueous and Ethanolic Extracts of the Male and Female Carica papaya Leaves on Some Pathogenic Bacteria

Bioengineering and Bioscience ◽

10.13189/bb.2017.050201 ◽

2017 ◽

Vol 5 (2) ◽

pp. 25-29

Author(s):

Awah N.S. ◽

Agu K.C. ◽

IIkedinma J.C. ◽

Uzoechi A.N. ◽

Eneite H.C. ◽

...

Keyword(s):

Pathogenic Bacteria ◽

Carica Papaya ◽

Antibacterial Activities ◽

Male And Female ◽

Ethanolic Extracts

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Thin-layer chromatographic analysis of flavonoids and total phenolics in methanolic and ethanolic extracts of Senna alata (L.) Roxb. (Fabales: Fabaceae)

Brazilian Journal of Biological Sciences ◽

10.21472/bjbs.030520 ◽

2016 ◽

Vol 3 (5) ◽

pp. 221

Author(s):

Solomon Oluwole Oladeji

Keyword(s):

Thin Layer ◽

Gallic Acid ◽

Chromatographic Analysis ◽

Antibacterial Activities ◽

Total Phenolic ◽

Antimicrobial Substances ◽

Soxhlet Apparatus ◽

Ethanolic Extracts ◽

Thin Layer Chromatographic Analysis ◽

Senna Alata

Herbal medicine has proved as a major source of therapeutic agents. They have been used to combat bacterial and fungal related infections and diseases. People depend on medicinal plants such as Senna alata (L.) Roxb. (Fabales: Fabaceae) without the knowledge of the chemical composition of the plant. Therefore, there is a need to examine the bioactive components that are present in S. alata. The methanolic and ethanolic extracts were obtained using soxhlet apparatus and the concentrated extracts were purified using column chromatography. The fractions were eluted and investigated for the presence of total flavonoids and phenolics. The values of thin-layer chromatographic analysis for the leaf, flower and fruit extracts of S. alata when compared to gallic acid and quercetrin (standard) showed that the fractions contained a chemical component in the class of gallic acid and quercetrin, respectively. The presence of total phenolic and flavonoids could be responsible for the observed antifungal and antibacterial activities of the plant. It could be concluded that this plant is a natural source of antimicrobial substances of high importance and are useful in antiseptic and disinfectant formulation as well as in chemotherapy.

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In-vitro antioxidant and antimicrobial studies of ethanolic plant extracts of P. granatum, O. stamineus, A. bilimbi, M. nigra, and E. longifolia

Current Pharmaceutical Biotechnology ◽

10.2174/1389201022666210615113854 ◽

2021 ◽

Vol 22 ◽

Author(s):

Essam A. Makky ◽

Manaf AlMatar ◽

Mahmood H. Mahmood ◽

Khoo Xu Wen ◽

Tan Boon Gaik Qi

Keyword(s):

Plant Extracts ◽

Total Concentration ◽

Antioxidant Properties ◽

Reducing Power ◽

Antibacterial Activities ◽

Diffusion Method ◽

Disc Diffusion ◽

Disc Diffusion Method ◽

Ethanolic Extracts ◽

Mic Value

Background: Acne is a serious skin problem that affects mostly adolescents. The topical and systematic therapies are effective but could lead to several side effects and the emergence of antibacterial resistance of the acne-causing bacteria. Plant resources have been used as traditional medicine for centuries and can be the alternative therapies for acne treatment. Antioxidants are compounds that can prevent or delay the oxidation of substrates when present in low concentrations. Antioxidants are usually involved in several mechanisms of action, including the inhibition of free radical generation, enhancement of the scavenging capacity against free radicals, and reducing power. Methods: In this study, three antioxidant assays, DPPH, ABTS, and FRAP were used to evaluate the antioxidant properties of the ethanolic extracts of five plant extracts (A. bilimbi, M. nigra, O. stamineus, P. granatum, and E. longifolia). Furthermore, the study aimed to identify the most potent plant extracts and their combination which could provide better antibacterial activities against acne-causing bacteria. Ethanolic extracts of A. bilimbi, O. stamineus, M. nigra, P. granatum, and E. longifolia were prepared by the Ultrasound-Assisted Extraction (UAE) technique. Their phytochemical contents were screened using several biochemical tests and GC-MS analysis. Results: The study found that only the extracts of P. granatum and O. stamineus exhibited good antioxidant activity using DPPH assay (82.37% and 81.72% μg/mL respectively) and in ABTS assay (87.25% and 88.85% μg/mL respectively); their observed FRAP values were 115.1667 ± 5.6182 and 41.3860 ± 2.4583 μg/mL, respectively. The preliminary antibacterial screening using the disc diffusion method showed that P. granatum and O. stamineus were the most potent extracts; hence, both extracts were combined at the ratios of 1:1, 1:2, and 2:1 to a total concentration of 400 mg/mL. Discussion: The antibacterial efficacy of the mixture was studied using the disc diffusion method and the MIC value was determined. Both S. aureus and S. epidermidis were sensitive to all the individual and combined extracts but C. acnes was resistant to all of them. Conclusion: The antibacterial activity of the combined extracts against S. aureus showed a synergistic effect at the ratio of 2:1 with the inhibitory zone diameter of 18.00 ± 1.00 mm and MIC value of 12.5 mg/mL; however, antagonistic effects were observed against S. epidermidis while no effect was noted against C. acnes. Therefore, all the selected plant extracts exhibited antibacterial activities against certain bacteria and their effects may be enhanced by combining the plant extracts.

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Comparative Antibacterial Efficacy of Vitellaria paradoxa (Shea Butter Tree) Extracts Against Some Clinical Bacterial Isolates

Notulae Scientia Biologicae ◽

10.15835/nsb739617 ◽

2015 ◽

Vol 7 (3) ◽

pp. 264-268 ◽

Cited By ~ 1

Author(s):

Kamoldeen Abiodun AJIJOLAKEWU ◽

Fola Jose AWARUN

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Antimicrobial Activity ◽

Klebsiella Pneumoniae ◽

Bacterial Pathogens ◽

Stem Bark ◽

Antibacterial Activities ◽

Shea Butter ◽

Vitellaria Paradoxa ◽

Ethanolic Extracts

The antibacterial activities of the ethanolic extracts of seed, leaf and stem bark of Vitellaria paradoxa were investigated. The extracts were tested against three clinical bacterial pathogens, Staphylococcus aureus, Escherichia coli and Klebsiella pneumoniae using the agar diffusion and the broth dilution techniques. Ethanolic extracts of the plant parts showed activity against all the bacterial pathogens tested. At the highest extract concentration (200 mg/ml), the leaf extract exhibited the highest antimicrobial activity, while no activity was detected at the lowest concentration (3.13 mg/ml) against the tested isolates. Escherichia coli and Staphylococcus aureus were more susceptible to all extracts of V. paradoxa, while Klebsiella pneumoniae showed the least sensitivity. The efficacy of ethanolic extracts of Vitellaria paradoxa was compared to a commercial antibiotic streptomycin. There were differences in the minimum inhibitory concentration (MIC) of all the Vitellaria paradoxa ethanolic extracts with respect to the type of organism. All extracts exhibited bacteriostatic effects against the tested organisms at the experimented concentrations. Qualitative phytochemical screening of the extracts revealed the presence of saponins, tannins and alkaloids as the active principles of Vitellaria paradoxa's antimicrobial activity. V. paradoxa could be used as a potential source of antibiotic substance for a drug development.

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Invitro Antimicrobial Activities of Mitracarpus scaber Against Some Common Bacteria of Aquatic Origin

Media Kedokteran Hewan ◽

10.20473/mkh.v32i3.2021.119-130 ◽

2021 ◽

Vol 32 (3) ◽

pp. 119

Author(s):

Abdullateef Abiodun Ajadi ◽

Benjamin Emikpe ◽

Ahmed Akeem

Keyword(s):

Antimicrobial Properties ◽

Ethanolic Extract ◽

Antibacterial Activities ◽

Antimicrobial Activities ◽

Phytochemical Analysis ◽

Ethanolic Extracts ◽

Zones Of Inhibition ◽

Mitracarpus Scaber

Some plants have been reported to be of medicinal values and reserve some antimicrobial properties. One of such plants is Mitracarpus scaber and its effect on bacterial growth is evaluated. The study aimed at evaluating the phytochemical analyses and antimicrobial potentials of Mitracarpus scaber against aquatic bacteria including Aeromonas, Bacillus, Vibrio and Staphylococcus Spp. Leaves of Mitracarpus scaber were collected, washed and air dried and phytochemical analysis and antimicrobial investigation of ethanolic and aqueous extracts of the leaves were carried out against a panel of bacteria isolated from diseased catfish from various farms. The phytoconstituents detected include saponins, tannins, flavonoids, tarpenoids, steroids, anthraquinones and alkaloids in both aqueous and ethanolic extracts. Both aqueous and ethanolic extracts of M. scaber showed varying degree of antibacterial activities but ethanolic extract showed a higher activity against the pathogens tested. The ethanolic extract had zones of inhibition similar to that of standard antibiotics (enrofloxacin) across all tested microbes. The lowest minimum inhibitory concentration of ethanolic extract of M. scaber was against Bacillus sp with 10mg/ml while the highest was 85mg/ml against Staphylococcus species. The results of the assays showed promising evidences that M. scaber is a potential antibacterial agent against aquatic microbes. However, further studies are recommended to fractionate its constituents and determine the in vitro and in vivo anti-microbial activities and the exact mechanism of action of the constituents.

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Antibacterial activities of methanolic and ethanolic extracts of the local plant, Uziza (Pipe guineense)

International Journal of Natural and Applied Sciences ◽

10.4314/ijonas.v2i1.36047 ◽

2006 ◽

Vol 2 (1) ◽

Cited By ~ 2

Author(s):

FOU Osuala ◽

SO Anyadoh

Keyword(s):

Antibacterial Activities ◽

Ethanolic Extracts ◽

Local Plant

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In vitro antimicrobial activity of ethanolic extracts obtained from Ficus spp. leaves against the fish pathogen Aeromonas hydrophila

Archives of Polish Fisheries ◽

10.1515/aopf-2016-0019 ◽

2016 ◽

Vol 24 (4) ◽

pp. 219-230 ◽

Cited By ~ 7

Author(s):

Halyna Tkachenko ◽

Lyudmyla Buyun ◽

Elżbieta Terech-Majewska ◽

Zbigniew Osadowski

Keyword(s):

Antimicrobial Activity ◽

Aeromonas Hydrophila ◽

Bacterial Infections ◽

Antibacterial Properties ◽

Antibacterial Activities ◽

Diffusion Method ◽

Moderate Activity ◽

In Vitro Antimicrobial Activity ◽

Ethanolic Extracts

Abstract The main goal of this study was to determine in vitro antimicrobial activity of ethanolic extracts obtained from the leaves of various Ficus species against Aeromonas hydrophila isolated locally from infected rainbow trout (Oncorhynchus mykiss Walbaum) with the aim of providing scientific rationale for the use of the plant in the treatment of bacterial infections induced by Aeromonas spp. in fish. Antimicrobial susceptibility testing was done on Muller-Hinton agar with the disc diffusion method. In the present study, most ethanolic extracts proved effective against the A. hydrophila tested, with 10-12 mm inhibition zones observed. A. hydrophila demonstrated the highest susceptibility to F. pumila. Among various species of Ficus with moderate activity against A. hydrophila, the highest antibacterial activities were noted for F. benghalensis, F. benjamina, F. deltoidea, F. hispida, and F. lyrata. Thus, Ficus can be used as a natural antiseptic and antimicrobial agent in veterinary practice. Further investigations need to be conducted to isolate and identify the bioactive compounds that can then be subjected to detailed pharmacological studies and the development of clinical applications. The alarming rate of increasing resistance in bacterial pathogens in aquaculture environments means that medicinal plants with antibacterial properties are very important as natural resources of new active compounds.

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