scholarly journals Radio-sensitivity of irradiated seed, plantlets, callus, and in vitro leaves from Indigofera zollingeriana Miq by gamma rays

2021 ◽  
Vol 913 (1) ◽  
pp. 012061
Author(s):  
J I Royani ◽  
Sudarsono ◽  
L Abdullah ◽  
S I Aisyah
Keyword(s):  
Gamma Rays ◽  
Growth Parameters ◽  
Curve Fit ◽  
Indigofera Zollingeriana ◽  
Radio Sensitivity

Abstract Test of radio-sensitivity is important to use as a recognizable effect of radiation. The optimal doses usually create maximum variability. Radio-sensitivity of each part of plants of Indigofera zollingeriana Miq to gamma rays had no report yet. This research aimed to know radio-sensitivity, optimal doses, and growth of M1 generation from each material of I. zollingeriana irradiated with gamma rays. Seed, plantlets, callus, and in vitro leaves were tested for radio sensitivity by gamma rays. Doses level used of Gamma rays were: 0 until 500 Gy. The value of LD50 of each material was evaluated using Curve-fit Analysis. Growth parameters from each material were observed for six weeks after planting and analyzed using IBM SPSS 22. Research showed that the sensitivity of each doses level was different for each source of the material plant. The seed had radio-sensitivity of gamma rays at dose 183.988 Gy, plantlet at dose 253.677 Gy, callus could not calculate, and in vitro leaves at dose 242.241 Gy.

scholarly journals Salt Tolerance, Morphological and Anatomical Responses of in vitro Indigofera zollingeriana Miq. Seedling

2021 ◽  
Vol 5 (4) ◽  
pp. 949-957
Author(s):  
Çiğdem Alev ÖZEL ◽  
Siti MAESAROH
Keyword(s):  
Salinity Stress ◽  
Cross Sections ◽  
Nutritional Value ◽  
Culture Medium ◽  
Growth Parameters ◽  
Lethal Dose ◽  
Indigofera Zollingeriana ◽  
The Tropics ◽  
Tolerance To Salinity

The species belonging to genus Indigofera with high nutritional value and tolerance against abiotic stresses are widely distributed in the tropics to the subtropic areas world over. In this study, two years old stored seeds of I. zollingeriana, a potential forage used in Indonesia, were evaluated for their tolerance to salinity stress. In the first step, the morphology and anatomy of I. zollingeriana seedlings under in vitro salinity stress level of 20-120 mM NaCl were investigated after 14 days. In the second step, the 3 days old seedlings were transferred to several concentrations of NaCl (140-300 mM) to estimate Ld50 (lethal dose). Several concentrations of gibberalic acid (GA3) were applied to the previous estimated Ld50 (228 mM NaCl) medium for alleviating seedlings damage. It was estimated that the increasing concentration of NaCl caused reduction in the evaluated plant growth parameters and changed anatomy of the root and stem cross sections. The 100% mortality of the seedlings was noted after4 weeks on the medium containing 300 mM NaCl. Treatment of seedlings with  <0.25 mg L-1 GA3 +228 mM NaCl (Ld50)   in the culture medium was effective to  reduce root damage for 4 weeks.   Treatment of seedlings with >2.5 mg L-1 GA3 + 228 mM NaCl (Ld50) showed adverse effects in controlling damage by necrosis and blackening of roots and stems.

Lethal Dose (LD50) Fixation and Sensitivity of Fenugreek (Trigonellafoenum-graecumL.) to Gamma Radiation for Induction of Mutation

2017 ◽  
Vol 4 (04) ◽  
Author(s):  
ANURADHA PATEL ◽  
POONAM VERMA ◽  
SHARDA CHOUDHARY ◽  
ARVIND KUMAR VERMA
Keyword(s):  
Genetic Variability ◽  
Gamma Rays ◽  
Growth Parameters ◽  
Seedling Survival ◽  
Lethal Dose ◽  
Germination Percentage ◽  
Mutation Breeding ◽  
Vigour Index ◽  
Small Flower ◽  
Dose Dependent

Fenugreek (Trigonella foenum-graecumL.) is an annual crop, mainly used as a spiceand leafy vegetable crop in many parts of the world. Classical breeding in fenugreek is restricted due to its low genetic variability and small flower size which hamper manual emasculation and pollination. Mutation breeding is an effective way to enrich genetic variability in crop plants. An experiment was conducted to determine the lethal dose of the physical mutagen gamma rays in fenugreek. The dry seeds of fenugreek were exposed to different doses of gamma rays i.e. 150Gy, 200Gy, 250Gy, 300Gy and 350Gy. These irradiated seeds were sown in the Petri plates with non-irradiated seeds (control). As the dose of gamma rays increased, there was a decrease in germination percentage, seedling survival, root length, shoot length and vigour index. Among five doses of gamma rays, the maximum seed germination was observed at lowest dose 150Gy (93%), followed by 200Gy (83%), 250Gy (76%), 300Gy (76%) and 350Gy (64%). The seedling survival was decreased from 90% (in control) to 56% in 350Gy dose of gamma rays. The gamma rays dose of 150Gy gave stimulatory effect on seedlings growth. The growth parameters were dose dependent, as the dose of gamma rays increased from 200Gy to 350Gy. The gamma rays dose of 350Gy showed 64% seeds germination and 56% of seedlings survival. Therefore, it is concluded that the LD50 dose for fenugreek is close to 350Gy. This information would be highly useful for initiating mutation breeding programme in fenugreek

scholarly journals In vitro Osmotic Stress Memory in Chrisanthemum hybridum: Structural and Physiological Responses

2017 ◽  
Vol 27 (2) ◽  
pp. 161-169 ◽  
Author(s):  
Lidiia Samarina ◽  
Valentina Malyarovskaya ◽  
Yulija Abilfazova ◽  
Natalia Platonova ◽  
Kristina Klemeshova ◽  
...  
Keyword(s):  
Osmotic Stress ◽  
Relative Water Content ◽  
Soluble Protein ◽  
Growth Parameters ◽  
Physiological Responses ◽  
Proline Content ◽  
Stress Exposure ◽  
Stress Memory ◽  
Relative Water

Structural and physiological responses of chrysanthemum to repeated osmotic stress were studied. Plants were cultured for 2 weeks (for each stress1 and stress 2) on half MS supplemented with mannitol 100 mM (Treatment I) and 200 mM (Treatment II). First stress inhibited growth parameters stronger than second stress in treatment I. In treatment II both stress events strongly inhibited growth parameters of micro‐shoots. Proline content exceeded control 6 ‐ 8 times after 1st stress, and 2 ‐ 5 times after the 2nd stress in treatments I and II, respectively. Soluble protein was accumulated in leaves during both stress exposures, and 2 ‐ 2.5 times exceeded control after the 2nd stress. Relative water content in both treatments increased after the 2nd stress exposure. In treatment II chlorophyll а and carotenoids contents were 8.78 and 4.62 mg/g comparing to control (4.21 and 2.25 mg/g, respectively) after the 1st stress. But after the 2nd stress there was no difference with control.Plant Tissue Cult. & Biotech. 27(2): 161-169, 2017 (December)

Neurotoxicity of Seven MEIC Chemicals Evaluated in Organotypic Cultures of Chick Embryonic Dorsal Root Ganglia

1997 ◽  
Vol 25 (3) ◽  
pp. 303-309
Author(s):  
Václav Mandys ◽  
Katerina Jirsová ◽  
Jirí Vrana
Keyword(s):  
Toxic Effect ◽  
Neurite Outgrowth ◽  
Dorsal Root Ganglia ◽  
Dorsal Root ◽  
Growth Parameters ◽  
In Vitro Cytotoxicity ◽  
Organotypic Cultures ◽  
Response Curves ◽  
Agar Culture

The neurotoxic effects of seven selected Multicenter Evaluation of In Vitro Cytotoxicity programme chemicals (methanol, ethanol, isopropanol, sodium chloride, potassium chloride, iron [II] sulphate and chloroform) were evaluated in organotypic cultures of chick embryonic dorsal root ganglia (DRG), maintained in a soft agar culture medium. Two growth parameters of neurite outgrowth from the ganglia — the mean radial length of neurites and the area of neurite outgrowth — were used to evaluate the toxicities of the chemicals. Dose-dependent decreases of both parameters were observed in all experiments. IC50 values (the concentration causing 50% inhibition of growth) were calculated from the dose-response curves established at three time-points during culture, i.e. 24, 48 and 72 hours. The lowest toxic effect was observed in cultures exposed to methanol (the IC50 ranging from 580mM to 1020mM). The highest toxic effect was observed in cultures exposed to iron (II) sulphate (the IC50 ranging from 1.2mM to 1.7mM). The results of other recent experiments suggest that organotypic cultures of DRG can be used during in vitro studies on target organ toxicity within the peripheral nervous system. Moreover, these cultures preserve the internal organisation of the tissue, maintain intercellular contacts, and thus reflect the in vitro situation, more precisely than other cell cultures.

scholarly journals Impact of gamma irradiation pretreatment on biochemical and molecular responses of potato growing under salt stress

2021 ◽  
Vol 8 (1) ◽  
Author(s):  
Elhamahmy Ali Mohamed ◽  
Elsadany Osama ◽  
Eid Manal ◽  
Abdelazeem Samah ◽  
Gerish Salah ◽  
...  
Keyword(s):  
Salt Tolerance ◽  
Genetic Marker ◽  
Gamma Rays ◽  
Salt Resistance ◽  
Base Pairs ◽  
Potato Genotype ◽  
Potato Callus ◽  
Primer Sequence ◽  
Significant Increment

Abstract Background Previous literatures revealed that gamma rays have an increasing effect on salt tolerance in different plants. In vitro experiment was conducted to study the effect of gamma rays (20 Gray) on salt tolerance of four potato cultivars (Lady Rosetta, Diamante, Gold, and Santana). Results Gamma-treated Santana plantlets were more tolerant to salinity as compared to other cultivars. It showed a significant increment of fresh weight (250% over the untreated). Gamma-treated plantlets of Lady Rosetta, Diamante, and Gold showed higher activity of peroxidase (POD) and polyphenol oxidase (PPO). Isoenzymes analysis showed an absence of POD 3, 4, and 5 in Gold plantlets. The dye of most PODs and PPOs bands were denser (more active) in gamma-treated plantlets of Santana as compared to other cultivars. Both gamma-treated and untreated plantlets showed the absence of PPO1 in Lady Rosetta and Diamante, and PPO 3, 4, and 5 in Gold plantlets. Genetic marker analysis using ISSR with six different primers showed obvious unique negative and positive bands with different base pairs in mutant plantlets as compared to the control, according to primer sequence and potato genotype. The 14A primer was an efficient genetic marker between mutated and unmutated potato genotypes. Santana had a unique fingerprint in the 1430-pb site, which can be a selectable marker for the cultivar. An increment in genetic distance between Gold cultivar and others proved that the mutation was induced because of gamma rays. Conclusion We assume that irradiation of potato callus by 20-Gy gamma rays is an effective process for inducing salt resistance. However, this finding should be verified under field conditions. Graphic Abstract

scholarly journals Rhizosphere-enriched microbes as a pool to design synthetic communities for reproducible beneficial outputs

2019 ◽  
Author(s):  
Maria-Dimitra Tsolakidou ◽  
Ioannis A Stringlis ◽  
Natalia Fanega-Sleziak ◽  
Stella Papageorgiou ◽  
Antria Tsalakou ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Fungal Pathogens ◽  
Growth Parameters ◽  
Tomato Plants ◽  
Beneficial Effects ◽  
Negative Effect ◽  
Tomato Growth ◽  
In Vitro Antifungal Activity

Abstract Composts represent a sustainable way to suppress diseases and improve plant growth. Identification of compost-derived microbial communities enriched in the rhizosphere of plants and characterization of their traits, could facilitate the design of microbial synthetic communities (SynComs) that upon soil inoculation could yield consistent beneficial effects towards plants. Here, we characterized a collection of compost-derived bacteria, previously isolated from tomato rhizosphere, for in vitro antifungal activity against soil-borne fungal pathogens and for their potential to change growth parameters in Arabidopsis. We further assessed root-competitive traits in the dominant rhizospheric genus Bacillus. Certain isolated rhizobacteria displayed antifungal activity against the tested pathogens and affected growth of Arabidopsis, and Bacilli members possessed several enzymatic activities. Subsequently, we designed two SynComs with different composition and tested their effect on Arabidopsis and tomato growth and health. SynCom1, consisting of different bacterial genera, displayed negative effect on Arabidopsis in vitro, but promoted tomato growth in pots. SynCom2, consisting of Bacilli, didn't affect Arabidopsis growth, enhanced tomato growth and suppressed Fusarium wilt symptoms. Overall, we found selection of compost-derived microbes with beneficial properties in the rhizosphere of tomato plants, and observed that application of SynComs on poor substrates can yield reproducible plant phenotypes.

scholarly journals Acceleration Breaks the Cells Defense Mechanisms against Vibration in Anthemis gilanica Calli

2021 ◽  
Vol 2021 ◽  
pp. 1-12
Author(s):  
Halimeh Hassanpour ◽  
Vahid Niknam ◽  
Sadaf Salami
Keyword(s):  
Protein Content ◽  
Defense Mechanisms ◽  
Growth Parameters ◽  
Resonance Effect ◽  
Total Carbohydrate ◽  
High Acceleration ◽  
Acceleration Amplitude ◽  
Relative Water ◽  
Stress Damage

Vibration is a mechanical stress which happens in nature and affects many biological aspects of plants. In this research, the effect of acceleration and vibration was investigated on some physiological and biochemical responses of Anthemis gilanica in vitro. Calli were induced from leaf (LS) and root segments (RS) and were applied to different frequencies of vibrations (0, 50, and 100 Hz) and accelerations (1, 2, and 4 g) on the A. gilanica calli for 30 min. Results showed that vibration significantly increased relative water content (RWC), growth parameters, protein and proline contents, ascorbate peroxidase (APX), peroxidase (POX), and superoxide dismutase (SOD) activities and decreased total carbohydrate, malondialdehyde (MDA), H2O2 contents, and polyphenol oxidase (PPO) activity in both LS and RS calli. Inversely, increase of acceleration in vibrated calli decreased growth parameters, RWC, protein content, and POX activity and induced proline and carbohydrate accumulations, SOD, APX, and PPO activities as compared to vibration alone. Different responses of two callus types were observed, and the highest growth, protein content, and membrane stability were observed in LS calli as compared to RS calli. It found that high acceleration amplitude intensified the resonance effect of vibration by induction of lipid peroxidation and oxidative stress damage in A. gilanica.

scholarly journals Influence of salt stress on plants of poplar clone "INRA 353-38" and willow clone "Zhytomyrska-1" in in vitro culture

2020 ◽  
Vol 83 (4) ◽  
pp. 43-49
Author(s):  
Yu. Khoma ◽  
L. Khudolieieva ◽  
N. Kutsokon
Keyword(s):  
Salt Stress ◽  
Sodium Chloride ◽  
In Vitro Culture ◽  
Growth Parameters ◽  
Hybrid Poplar ◽  
Salt Resistance ◽  
Soil Salinization ◽  
In Vitro Cultivation ◽  
Abiotic Factor

Soil salinization is an important abiotic factor negatively affecting plant growth, development and productivity. Fast-growing poplar and willow trees are important plants for bioenergy production demonstrating varying degrees of adaptation to different habitats. The study of salt resistance in different clones of poplars and willows will reveal genotypes that can be planted in saline soils for producing biomass for the bioenergy industry. Therefore, the aim of the study was to investigate the effects of salt stress on poplar plants of clone 'INRA 353-38' (Populus tremula × P. tremuloides) and willow clone 'Zhytomyrska – 1' (Salix sp.) under in vitro culture. For this purpose the plants were cultivated on MS nutrient medium with the addition of sodium chloride in concentrations 25 mM, 50 mM and 100 mM. The control plants were grown on the sodium chloridefree medium. The plant status (with a 4-score scale), the intensity of their growth (by shoot length) and rooting capacity (by the number of roots) were assessed on the 10th and the 30th day of cultivation. The results obtained indicate a high level of sensitivity to sodium chloride of both studied clones under in vitro cultivation. But the willow 'Zhytomyrska – 1' had a higher sensitivity to salt stress comparing to hybrid polar 'ІNRA 353-38' since growth parameters of willow were significantly decreased even under the concentration of sodium chloride 50 mM, and in the case of short term influence (10 days) of the highest concentration of sodium chloride (100 mM) all willow plants terminated their growth and quickly died. The growth parameters of hybrid poplar were declined within a month, mainly under the highest concentration of sodium chloride, but even under such conditions some part of the shoots were able to survive.

scholarly journals Efficacy of Experimental Mouth Rinses on Caries-Related Biofilms in vitro

2021 ◽  
Vol 2 ◽  
Author(s):  
Josiana Steiger ◽  
Olivier Braissant ◽  
Tuomas Waltimo ◽  
Monika Astasov-Frauenhoffer
Keyword(s):  
Growth Rate ◽  
Antimicrobial Agents ◽  
Growth Parameters ◽  
Screening Method ◽  
Maximum Growth ◽  
Maximum Growth Rate ◽  
Screening Process ◽  
Mouth Rinses ◽  
Amine Fluoride

This study assessed the efficacy of tin and Polyethylenglycol (PEG-3) tallow aminopropylamine in different concentrations on Streptococcus mutans (S. mutans) biofilms to establish a new screening process for different antimicrobial agents and to gain more information on the antibacterial effects of these agents on cariogenic biofilms. Isothermal microcalorimetry (IMC) was used to determine differences in two growth parameters: lag time and growth rate; additionally, reduction in active biofilms was calculated. Experimental mouth rinses with 400 and 800 ppm tin derived from stannous fluoride (SnF2) revealed results (43.4 and 49.9% active biofilm reduction, respectively) similar to meridol mouth rinse (400 ppm tin combined with 1,567 ppm PEG-3 tallow aminopropylamine; 55.3% active biofilm reduction) (p &gt; 0.05), while no growth of S. mutans biofilms was detected during 72 h for samples treated with an experimental rinse containing 1,600 ppm tin (100% active biofilm reduction). Only the highest concentration (12,536 ppm) of rinses containing PEG-3 tallow aminopropylamine derived from amine fluoride (AmF) revealed comparable results to meridol (57.5% reduction in active biofilm). Lower concentrations of PEG-3 tallow aminopropylamine showed reductions of 16.9% for 3,134 ppm and 33.5% for 6,268 ppm. Maximum growth rate was significantly lower for all the samples containing SnF2 than for the samples containing control biofilms (p &lt; 0.05); no differences were found between the control and all the PEG-3 tallow aminopropylamine (p &gt; 0.05). The growth parameters showed high reproducibility rates within the treated groups of biofilms and for the controls; thus, the screening method provided reliable results.

scholarly journals A statistical framework for assessing pharmacological responses and biomarkers using uncertainty estimates

eLife ◽  
2020 ◽  
Vol 9 ◽  
Author(s):  
Dennis Wang ◽  
James Hensman ◽  
Ginte Kutkaite ◽  
Tzen S Toh ◽  
Ana Galhoz ◽  
...  
Keyword(s):  
Cell Lines ◽  
Dose Response ◽  
Biomarker Discovery ◽  
Statistical Framework ◽  
Curve Fit ◽  
Uncertainty Estimates ◽  
Drug Response Biomarkers ◽  
Novel Biomarkers ◽  
Response Biomarkers

High-throughput testing of drugs across molecular-characterised cell lines can identify candidate treatments and discover biomarkers. However, the cells’ response to a drug is typically quantified by a summary statistic from a best-fit dose-response curve, whilst neglecting the uncertainty of the curve fit and the potential variability in the raw readouts. Here, we model the experimental variance using Gaussian Processes, and subsequently, leverage uncertainty estimates to identify associated biomarkers with a new Bayesian framework. Applied to in vitro screening data on 265 compounds across 1074 cancer cell lines, our models identified 24 clinically established drug-response biomarkers, and provided evidence for six novel biomarkers by accounting for association with low uncertainty. We validated our uncertainty estimates with an additional drug screen of 26 drugs, 10 cell lines with 8 to 9 replicates. Our method is applicable to any dose-response data without replicates, and improves biomarker discovery for precision medicine.

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