scholarly journals PSVI-13 Effects of Saccharomyces cerevisiae and Aspergillus-based enzyme extracts on rumen fermentation, fiber digestibility, and plasma metabolome of beef steers fed red clover/orchard hay

2020 ◽  
Vol 98 (Supplement_4) ◽  
pp. 434-434
Author(s):  
Ibukun M Ogunade ◽  
Devan M Compart ◽  
Andres A Pech-Cervantes
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Dry Matter ◽  
Rumen Fluid ◽  
Synergistic Effects ◽  
Plasma Concentrations ◽  
Red Clover ◽  
Rumen Fermentation ◽  
Beef Steers ◽  
Fiber Digestibility

Abstract Saccharomyces cerevisiae (SC) and Aspergillus-based enzyme extracts (ABE) are respectively fed to improve gut health and forage digestibility in ruminants. Also, cells of SC contain polyamines which can regulate the immune function. This study evaluated the synergistic effects of SC and ABE on rumen fermentation, plasma polyamine concentrations, and in vitro fiber digestibility of beef steers fed red clover/orchard hay. Eight rumen-cannulated Holstein steers were assigned to 4 treatments arranged in a 4 × 4 Latin square design with four 21-d periods. Dietary treatments were (1) CON (basal diet without additive), (2) SC (CON + 15 g/d of live SC; PMI, Arden Hills, MN, (3) ABE (5 g of ABE; PMI, Arden Hills, MN, USA) and (4) SC+ABE (15 g/d of live SC + 5 g of ABE). On d 21, blood samples were collected before morning feeding for analysis of plasma polyamines (spermines, spermidine, and putrescine). Rumen fluid samples were collected 3, 6, and 9 hours after feeding for analysis of VFA and in vitro dry matter and fiber digestibility. The model included the effects of treatment, period, and random effects of cow and square. Compared with CON, neither SC nor ABE had effects (P > 0.10) on ruminal VFA profile. In contrast, SC+ABE increased (P ≤ 0.05) the ruminal concentrations of acetate, butyrate, valerate and total VFA concentrations. In vitro dry matter and fiber digestibilities were increased (P < 0.05) by SC+ABE, but not by SC or ABE alone. Both SC and SC+ABE increased (P < 0.05) plasma concentrations of spermidine and spermine. These results suggest that SC supplementation with or without ABE increased plasma concentrations of polyamines; however, SC and ABE synergistically improved the rumen fermentation and in vitro fiber and DM digestibility.

scholarly journals Effects of Geraniol and Camphene on in Vitro Rumen Fermentation and Methane Production

2017 ◽  
Vol 48 (2) ◽  
pp. 63-69
Author(s):  
M. Joch ◽  
V. Kudrna ◽  
B. Hučko
Keyword(s):  
Methane Emission ◽  
Methane Production ◽  
Dry Matter ◽  
Volatile Fatty Acids ◽  
Rumen Fluid ◽  
Positive Control ◽  
Rumen Fermentation ◽  
Dry Matter Digestibility

AbstractThe objective of this study was to determine the effects of geraniol and camphene at three dosages (300, 600, and 900 mg l-1) on rumen microbial fermentation and methane emission in in vitro batch culture of rumen fluid supplied with a 60 : 40 forage : concentrate substrate (16.2% crude protein, 33.1% neutral detergent fibre). The ionophore antibiotic monensin (8 mg/l) was used as positive control. Compared to control, geraniol significantly (P < 0.05) reduced methane production with increasing doses, with reductions by 10.2, 66.9, and 97.9%. However, total volatile fatty acids (VFA) production and in vitro dry matter digestibility were also reduced (P < 0.05) by all doses of geraniol. Camphene demonstrated weak and unpromising effects on rumen fermentation. Camphene did not decrease (P > 0.05) methane production and slightly decreased (P < 0.05) VFA production. Due to the strong antimethanogenic effect of geraniol a careful selection of dose and combination with other antimethanogenic compounds may be effective in mitigating methane emission from ruminants. However, if a reduction in total VFA production and dry matter digestibility persisted in vivo, geraniol would have a negative effect on animal productivity.

Effect of dietary forage: concentrate ratio on the behaviour, rumen fermentation and circulating concentrations of IGF-1, insulin, glucagon and metabolites of beef steers and their potential effects on carcass composition

1999 ◽  
Vol 68 (3) ◽  
pp. 533-546 ◽  
Author(s):  
C. L. Thorp ◽  
A. R. G.Wylie ◽  
R. W. J. Steen ◽  
C. Shaw ◽  
J. D. McEvoy
Keyword(s):  
Food Intake ◽  
Dry Matter ◽  
Rumen Fluid ◽  
Rumen Fermentation ◽  
Carcass Composition ◽  
Beef Steers ◽  
Restricted Diet ◽  
Lower Efficiency ◽  
Peripheral Tissues ◽  
Ad Libitum

AbstractIn an investigation of factors responsible for the lower efficiency of carcass lean gain seen previously in steers offered grass silage diets 18 Simmental × British Friesian steers (361 (s.e. 5-35) kg) were offered one of three diets: a perennial ryegrass silage ad libitum and alone (S) or supplemented with rolled barley at 300 g/kg of total dry matter (SC) or supplemented as described but restricted (SCr) in quantity so as to provide the same dry matter (DM) and digestible energy (DE) intakes as for S. Eating (Eb), ruminating (Rb), standing (Sb) or lying (Lb) behaviour was quantified during four 24-h periods of manual observation. Eb was noted in more detail in a second experiment using computerized Calan-Broadbent gates and load cells to monitor times and rates of eating. Blood was taken via temporary indwelling jugular catheters at 30 to 60 min intervals on each of 4 days 1 month apart. Rumen fluid was sampled hourly for three 24-h periods from three rumen-cannulated steers given the same three diets in a separate 3 x 3 change-over design experiment.Steers offered the restricted diet SCr ate most of their food in one extended meal within 6 h of feeding while two peak eating periods (morning and evening) were observed in steers offered the other two diets. Steers offered SCr spent more time in Sb (P < 0.05), and less time in Eb (P < 0·001) and Rb (P < 0·05) activities than did animals offered the two diets ad libitum (SC and S). Mean 24 h insulin-like growth factor-1 (IGF-1) concentrations and postprandial insulin concentrations were significantly higher with diet SCr than with diet S (P < 0·001) despite equal daily DM and DE intakes from each. Insulin appearance in the jugular vein reflected the pattern of food intake on all treatments. Rumen fermentation characteristics were largely unaffected by diet. Mean 24 h rumen volatile fatty acid, pH and ammonia concentrations did not differ between diets but post-prandial rumen pH tended to be lower in animals offered the SC and SCr diets.Differences in patterns of food intake between animals offered food ad libitum and at a restricted level are likely to determine patterns of nutrient absorption and the secretion of nutritionally regulated splanchnic hormones. The higher proportions of Sb and Rb activities in steers offered the restricted diet represent an energy cost to these animals while the higher plasma IGF-1 and insulin concentrations also seen in these animals may collectively influence the partitioning of nutrients to the peripheral tissues and contribute to the increased efficiency of carcass lean deposition previously shown in animals offered such diets.

scholarly journals In vitro rumen methane output of forb species sampled in spring and summer

2012 ◽  
Vol 21 (2) ◽  
pp. 83-90 ◽  
Author(s):  
Peter James Purcell ◽  
Tommy M. Boland ◽  
Martin O'Brien ◽  
Pádraig O'Kiely
Keyword(s):  
Chemical Composition ◽  
Dry Matter ◽  
Rumen Fluid ◽  
Artificial Saliva ◽  
Rumen Fermentation ◽  
Urtica Dioica ◽  
Grass Silage ◽  
Lower Extent ◽  
In Vitro Rumen Fermentation

The chemical composition, in vitro rumen fermentation variables and methane (CH4) output of a range of common forb species sampled in spring and summer, and grass silage (14 treatments in total), were determined in this study. Dried, milled herbage samples were incubated in an in vitro rumen batch culture with rumen microbial inoculum (rumen fluid) and buffered mineral solution (artificial saliva) at 39 °C for 24 hours. All herbage chemical composition and in vitro rumen fermentation variables were affected (p<0.001) by treatment. Rumex obtusifolius (in spring and summer), Urtica dioica (summer) and Senecio jacobaea (summer) had lower (p<0.05) CH4 outputs relative to feed dry matter incubated compared with grass silage, reflecting their lower extent of in vitro rumen fermentation.

scholarly journals In vitro rumen fermentation characteristics and microbes of thin tail sheep given sweet potato (Ipomoea batatas L) biomass

2016 ◽  
Vol 21 (2) ◽  
pp. 83
Author(s):  
Asep Sudarman ◽  
M. Hayashida ◽  
M. Miralestari
Keyword(s):  
Organic Matter ◽  
Sweet Potato ◽  
Ipomoea Batatas ◽  
Dry Matter ◽  
Rumen Fluid ◽  
Block Design ◽  
Rumen Fermentation ◽  
Napier Grass ◽  
Sweet Potato Leaves

<p class="abstrak2">Sweet potato plant is produced in Indonesia in large quantities. Sweet potato leaves and stems can be used as a source of protein and the tuber can be used as an energy source. This study was aimed to analyze the effect of feeding of sweet potato biomass on the rumen fermentation characteristics, digestibility of dry matter and organic matter as well as rumen microbial population of thin tail sheep. This study used a randomized block design by applying four feed treatments, i.e., T0 (100% Napier grass), T1 (70% of Napier grass + 30% concentrate), T2 (50% of Napier grass + 50% sweet potato leaves), and T3 (70% sweet potato leaves + 30% sweet potato tuber) with three replicates. Rumen fluid samples were taken with stomach tube method using a vacuum pump.  Results showed that the T3 diet has higher (P&lt;0.05) digestibility of dry matter and organic matter, concentration of NH<sub>3</sub> and VFA, and rumen bacterial population than those of T0 and T1diets but similar to that of T2 diet. Rumen pH and protozoa population was not different among the treatments. It can be concluded that the use of sweet potato biomass can improve the quality of rumen fermentation of sheep.</p>

scholarly journals Application of Selected Inoculant Producing Antifungal and Fibrinolytic Substances on Rye Silage with Different Wilting Time

Processes ◽  
2021 ◽  
Vol 9 (5) ◽  
pp. 879
Author(s):  
Seong-Shin Lee ◽  
Jeong-Seok Choi ◽  
Dimas Hand Vidya Paradhipta ◽  
Young-Ho Joo ◽  
Hyuk-Jun Lee ◽  
...  
Keyword(s):  
Lactobacillus Plantarum ◽  
Dry Matter ◽  
Lactobacillus Brevis ◽  
Neutral Detergent Fiber ◽  
Dry Matter Digestibility ◽  
Fiber Digestibility ◽  
Aerobic Stability ◽  
Commercial Inoculant

This research was conducted to determine the effects of selected inoculant on the silage with different wilting times. The ryes were unwilted or wilted for 12 h. Each rye forage was ensiled for 100 d in quadruplicate with commercial inoculant (Lactobacillus plantarum sp.; LPT) or selected inoculant (Lactobacillus brevis 100D8 and Leuconostoc holzapfelii 5H4 at 1:1 ratio; MIX). In vitro dry matter digestibility and in vitro neutral detergent fiber digestibility were highest in the unwilted MIX silages (p < 0.05), and the concentration of ruminal acetate was increased in MIX silages (p < 0.001; 61.4% vs. 60.3%) by the increase of neutral detergent fiber digestibility. The concentration of ruminal ammonia-N was increased in wilted silages (p < 0.001; 34.8% vs. 21.1%). The yeast count was lower in the MIX silages than in the LPT silages (p < 0.05) due to a higher concentration of acetate in MIX silages (p < 0.05). Aerobic stability was highest in the wilted MIX silages (p < 0.05). In conclusion, the MIX inoculation increased aerobic stability and improved fiber digestibility. As a result of the wilting process, ammonia-N in silage decreased but ruminal ammonia-N increased. Notably, the wilted silage with applied mixed inoculant had the highest aerobic stability.

scholarly journals Reducing enteric methane production from buffalo (Bubalus bubalis) by garlic oil supplementation in in vitro rumen fermentation system

2021 ◽  
Vol 3 (2) ◽  
Author(s):  
Avijit Dey ◽  
Shyam Sundar Paul ◽  
Puran Chand Lailer ◽  
Satbir Singh Dahiya
Keyword(s):  
Environmental Pollution ◽  
Methane Production ◽  
Rumen Fluid ◽  
Bubalus Bubalis ◽  
Rumen Fermentation ◽  
Acetyl Esterase ◽  
Garlic Oil ◽  
Enteric Methane ◽  
In Vitro Rumen Fermentation

AbstractEnteric methane production contributes significantly to the greenhouse gas emission globally. Although, buffaloes are integral part of livestock production in Asian countries, contributing milk, meat and draft power, the contribution of enteric methane to environmental pollution attracts attention. The present study investigated the efficacy of garlic (Allium sativum) oil in reducing enteric methane production from buffaloes (Bubalus bubalis) by in vitro rumen fermentation. Garlic oil (GOL) was tested at four concentrations [0 (Control), 33.33 µl (GOL-1), 83.33 µl (GOL-2) and 166.66 µl (GOL-3) per litre of buffered rumen fluid] in 100-ml graduated glass syringes and incubated at 39℃ for 24 h for in vitro rumen fermentation study. Supplementation of GOL-1 increased (p < 0.05) total gas production in comparison with GOL-3; however, it remained comparable (p > 0.05) with control and GOL-2. Graded doses of garlic oil inclusions reduced (p < 0.001) methane concentration (%) in total gas and total methane production (ml/g DM), irrespective of concentrations. The feed degradability, volatile fatty acids and microbial biomass production (MBP) were not affected (p > 0.05) by GOL-1, but these tended to decrease in GOL-2 with marked reduction (p < 0.01) in GOL-3. The decrease (p < 0.01) in NH3–N concentration in fermentation fluid in the presence of garlic oil, irrespective of concentration, suggests reduced deamination by inhibiting rumen proteolytic bacterial population. The activities of ruminal fibrolytic enzymes (CMCase, xylanase, β-glucosidase, acetyl esterase) were not affected by lower dose (GOL-1) of garlic oil; however, reduction (p < 0.05) of these enzymes activity in rumen liquor was evident at higher doses (GOL-2 and GOL-3) of supplementation. This study shows positive impact of garlic oil supplementation at low dose (33.33 µl/l of rumen fluid) in reducing enteric methane production, thereby, abatement of environmental pollution without affecting feed digestibility.

scholarly journals 49 Effects of in-vitro digestibility in cannulated steers when supplemented different levels of glycerol

2020 ◽  
Vol 98 (Supplement_2) ◽  
pp. 55-56
Author(s):  
Noheli Gutierrez ◽  
Jamie A Boyd
Keyword(s):  
Dry Matter ◽  
Cold Water ◽  
Rumen Fluid ◽  
Latin Square ◽  
Optimal Level ◽  
Nutrient Digestibility ◽  
In Vitro Digestibility ◽  
Study Results ◽  
Different Levels

Abstract A study was conducted to evaluate effects of increasing concentration of food grade glycerol on rumen environment and nutrient digestibility. Three ruminally cannulated Jersey steers were used in this study. The study was conducted from March to May 2019. Experimental design was a 3x3 Latin square with a 2wk adjustment period followed by a 1wk collection period. Diet was coastal bermudagrass hay based. Different forage types were introduced in the incubation process to evaluate digestibility. Glycerol was administered once a day at 0, 15, or 20% of DMI (dry matter intake). dNDF (digestible NDF) and dDM (digestible dry matter) was determined using an ANKOM Daisy II incubator inoculated with 200g fresh rumen fluid and incubated for 12, 24, 48 and 72 h at 39°C. Each vessel contained ground forage samples in filter bags in triplicate. After incubation, filter bags were rinsed with cold water and dried for 24h in a 55°C forced air oven. Data were analyzed using the Proc MIXED procedure of SAS version 9.4. There was no difference dNDF in effect of different levels of glycerol between forage types by diet. But a numerical tendency was observed that dNDF was decreased at 20% inclusion rates in comparison to 0 and 15% inclusion of glycerol in the diet. Neither steer nor run was significantly different in the study. However as expected digestibility over time was significantly different (P &lt; 0.001). A significant increase was observed in DMI with the increased levels of glycerol in the diet (P = 0.003), both the 15% and 20% levels of glycerol increased in DMI in comparison to the control (0%). It appears based on these study results that digestibility may be inhibited, as levels of dietary glycerol increase in the diet and more work needs to be done to find the optimal level of glycerol supplementation.

The assessment of herbage legume varieties. II. In vitro digestibility, water soluble carbohydrate, crude protein and mineral content of primary growth of clover and lucerne

1966 ◽  
Vol 66 (3) ◽  
pp. 351-357 ◽  
Author(s):  
W. Ellis Davies ◽  
G. ap Griffith ◽  
A. Ellington
Keyword(s):  
White Clover ◽  
Crude Protein ◽  
Dry Matter ◽  
Red Clover ◽  
Water Soluble ◽  
Soluble Carbohydrates ◽  
Soluble Carbohydrate ◽  
In Vitro Digestibility ◽  
Primary Growth

The primary growth of eight varieties of three species–white clover (3), red clover (4) and lucerne (1)–was sampled at fortnightly intervals and the percentage dry matter, in vitro digestibility, crude protein, water soluble carbohydrates, P, Ca, K, Na and Mg were determined.Differences between species were nearly always significant and the general order of merit was white clover, red clover and lucerne. The exceptions were for dry-matter percentage where this order was reversed, and red clover had the lowest Na and highest Mg content.

scholarly journals Live Bacillus subtilis natto Promotes Rumen Fermentation by Modulating Rumen Microbiota In Vitro

Animals ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 1519
Author(s):  
Meinan Chang ◽  
Fengtao Ma ◽  
Jingya Wei ◽  
Junhao Liu ◽  
Xuemei Nan ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
16S Rrna ◽  
Rumen Fluid ◽  
Gene Sequencing ◽  
16S Rrna Gene Sequencing ◽  
Rumen Fermentation ◽  
Rrna Gene ◽  
Bacillus Subtilis Natto ◽  
Rrna Gene Sequencing

Previous studies have shown that Bacillus subtilis natto affects rumen fermentation and rumen microbial community structure, which are limited to detect a few microbial abundances using traditional methods. However, the regulation of B. subtilis natto on rumen microorganisms and the mechanisms of microbiota that affect rumen fermentation is still unclear. This study explored the effects of live and autoclaved B. subtilis natto on ruminal microbial composition and diversity in vitro using 16S rRNA gene sequencing and the underlying mechanisms. Rumen fluid was collected, allocated to thirty-six bottles, and divided into three treatments: CTR, blank control group without B. subtilis natto; LBS, CTR with 109 cfu of live B. subtilis natto; and ABS, CTR with 109 cfu of autoclaved B. subtilis natto. The rumen fluid was collected after 0, 6, 12, and 24 h of fermentation, and pH, ammonia nitrogen (NH3-N), microbial protein (MCP), and volatile fatty acids (VFAs) were determined. The diversity and composition of rumen microbiota were assessed by 16S rRNA gene sequencing. The results revealed LBS affected the concentrations of NH3-N, MCP, and VFAs (p < 0.05), especially after 12 h, which might be attributed to changes in 18 genera. Whereas ABS only enhanced pH and NH3-N concentration compared with the CTR group (p < 0.05), which might be associated with changes in six genera. Supplementation with live B. subtilis natto improved ruminal NH3-N and propionate concentrations, indicating that live bacteria were better than autoclaved ones. This study advances our understanding of B. subtilis natto in promoting ruminal fermentation, providing a new perspective for the precise utilization of B. subtilis natto in dairy rations.

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