PSI-5 Effects of Bacillus and Lactobacillus in Milk Replacer on Calf Health

2021 ◽  
Vol 99 (Supplement_1) ◽  
pp. 227-227
Author(s):  
Kenzie McAtee ◽  
Steven T Quantz ◽  
Tom Rehberger ◽  
Kellen Habib ◽  
Kristen Smith ◽  
...  
Keyword(s):  
Block Design ◽  
Health Indicators ◽  
Experimental Unit ◽  
Bacillus Species ◽  
Milk Replacer ◽  
Nasal Discharge ◽  
Bacterial Populations ◽  
Calf Health ◽  
Watery Stool ◽  
Milk Replacers

Abstract Stress in pre-ruminant calves can impact the functionality of the gastric epithelial lining, thereby impacting calf health during times of stress and potentially later in life. To mitigate this challenge, milk replacers frequently contain antibiotics to reduce the proliferation of harmful bacteria that may result in disease in pre-ruminant, milk supplemented calves. However, there is consumer and regulatory pressure to reduce the reliance on these antimicrobials and evaluate alternatives, such as probiotics, that may shift gut bacterial populations without impacting potential antimicrobial resistance. The objective of this study was to determine if supplementing calves with Lactobacillus and Bacillus species in milk replacer impacts pre-ruminant calf health. The project included 44 calves (50% Angus/50% Holstein) in a randomized complete block design, with calf serving as the experimental unit and age serving as a blocking factor. Calves were fed twice daily one of two treatments: 1) milk replacer with Lactobacillus and two Bacillus species at a total rate of 1.25 x 10^9 cfu/head/day; or 2) non-treated milk replacer. There were 22 replicates per treatment. To determine health status, calves were scored daily based on health indicators for fecal consistency or eye, nose, or ear normality. Health scores were ranked from 0 to 3, with 0 representing normal and 3 signaling severe symptoms, such as bilateral eye or nasal discharge, ear droop, or watery feces. Statistical analysis was performed using the GLIMMIX procedure of SAS. Overall, there was no evidence (P > 0.05) that Lactobacillus and Bacillus inclusion impacted any measured health parameter. Notably, approximately 50% of calves had fecal scores indicating some level of watery stool, but this was not impacted (P > 0.05) by probiotics. In summary, the inclusion of probiotics in calf milk replacer did not appear to impact calf health in the first 30 days.

159 Evaluation of Bacillus Subtilis PB6 Probiotic (CLOSTAT® 500) on Feedlot Phase Growth Performance, Efficiency of Dietary Net Energy Utilization, and Fecal and Subiliac Lymph Node Salmonella Prevalence

2021 ◽  
Vol 99 (Supplement_1) ◽  
pp. 119-120
Author(s):  
Zachary K Smith ◽  
Paul Rand R Broadway ◽  
Keith Underwood ◽  
Warren C Rusche ◽  
Julie Walker ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Lymph Node ◽  
Lymph Nodes ◽  
Growth Performance ◽  
Block Design ◽  
Average Daily Gain ◽  
Energy Utilization ◽  
Experimental Unit ◽  
Net Energy ◽  
Fecal Samples

Abstract Yearling beef steers (n = 238; initial BW=402 ± 31.2 kg) were used to evaluate a Bacillus subtilis probiotic on growth performance, dietary net energy (NE) utilization, carcass characteristics, and fecal and subiliac lymph node Salmonella prevalence during a 140-d finishing period. Steers were allotted to 24 pens (n = 9 to 10 steers/pen) and assigned to one of two treatments (12 pens/treatment): no probiotic (CON) or 0.50 g·steer-1·d-1 of a Bacillus subtilis PB6 probiotic (CLOSTAT® 500, Kemin Industries, Des Moines, IA; CLO). Steers were transitioned to a 90% concentrate diet (DM basis) over 14-d. Steers were fed once daily at 0700 h; bunks were managed according to a slick bunk management. Fecal samples were collected on d 1, 28, 56, 112, and 140 from each pen (n = 5 steers/pen) via rectal palpation and composited by pen for determination of Salmonella prevalence. Upon harvest, subiliac lymph nodes were obtained from 60 steers in CON and 57 steers in CLO. Data were analyzed as a randomized complete block design; pen was the experimental unit; α of 0.05 determined significance. No differences were detected (P ≥ 0.25) between treatments for live or carcass-adjusted average daily gain, dry matter intake, gain efficiency, dietary NE utilization, nor calculated dietary NE content based upon performance. No differences were detected between treatments for any carcass traits (P ≥ 0.15). Salmonella was not recovered in any fecal samples except on d 112, where steers from CLO had a numerically lower (P = 0.17; 8.3 vs. 25.0%) incidence of fecal Salmonella compared to CON and on d 140 fecal, where Salmonella incidence did not differ (P = 0.34; 0.0 vs. 8.3%) for CON and CLO, respectively. Salmonella was not recovered in any subiliac lymph nodes. These data indicate that CLO did not influence growth performance or Salmonella prevalence.

scholarly journals 251 Evaluation of Condensed Algal Residue Solubles as an Ingredient in Cattle Finishing Diets

2020 ◽  
Vol 98 (Supplement_3) ◽  
pp. 137-138
Author(s):  
Calvin Gibbons ◽  
Andrea K Watson ◽  
Galen E Erickson ◽  
Bradley M Boyd ◽  
Levi J McPhillips ◽  
...  
Keyword(s):  
Great Plains ◽  
Fixed Effects ◽  
Block Design ◽  
Random Effect ◽  
Distillers Grains ◽  
Experimental Unit ◽  
Omega 3 ◽  
Finishing Diets ◽  
Randomized Block Design ◽  
Algae Oil

Abstract Algae oil production for Omega-3 fatty acid supplementation yields a byproduct called Condensed Algal Residue Solubles (CARS; 25.4% DM, 19.3% CP, 8.3% Fat, 9.96% Na on DM basis), de-oiled algae cells with residual fermentation substrates. This study evaluated the use of CARS in feedlot finishing diets. Crossbreed steers, (n=480) were blocked and stratified by initial body weight (BW) into 4 blocks, and assigned randomly to treatments. Treatments were designed as a 2 x 3 factorial with 3 inclusions of CARS (0, 2.5, 5% of diet DM) and 2 different base diets representing Northern and Southern Great Plains diets. The Southern diets contained steam flaked corn and dry distillers grains while the Northern diets had dry rolled and high moisture corn with wet distillers grains. CARS replaced corn in both diets. All blocks were harvested after 148 days on feed. Performance data were analyzed as a randomized block design with CARS inclusion, base diet, and interactions as fixed effects, BW block as a random effect and pen (n=48) as the experimental unit. Orthogonal contrasts were used to test linear and quadratic effects of CARS inclusion. There were no significant interactions between CARS inclusion and diet type (P ≥ 0.49). Main effects of CARS indicated positive quadratic responses for carcass adjusted ADG, G:F, 12th rib back fat, yield grade (P < 0.01; increasing to 2.5% inclusion, decreasing at 5%) and hot carcass weight was both linear and quadratic (P ≤ 0.01 and P ≥ 0.06 respectively; 969, 977, 935 as CARS increased). Linear decrease in DMI, final adjusted BW and ribeye area (P ≤ 0.01) as CARS increased. Cattle fed the Southern diets had greater ADG and G:F compared to Northern diets (P < 0.01). Including 2.5% CARS in the diet improved feed efficiency in both Northern and Southern based feedlot diets.

172 Effects of Dietary Valine, Isoleucine, and Tryptophan Supplementations to Diets Containing Excess Leucine from Corn Protein on Growth Performance of Growing Pigs

2021 ◽  
Vol 99 (Supplement_1) ◽  
pp. 61-62
Author(s):  
Woongbi Bi Kwon ◽  
Jose A Soto ◽  
Hans H Stein
Keyword(s):  
Block Design ◽  
Control Diet ◽  
Average Daily Gain ◽  
Growing Pigs ◽  
Experimental Unit ◽  
Negative Effects ◽  
Final Body Weight ◽  
Corn Protein ◽  
Dietary Treatments ◽  
Daily Gain

Abstract An experiment was conducted to test the hypothesis that addition of Val, Ile, or Trp alone or in combination will reduce the negative effects of excess Leu in diets for growing pigs. A total of 288 growing pigs (28.6 ± 2.5 kg) were randomly assigned to 1 of 9 dietary treatments in a randomized complete block design. There were 2 barrows and 2 gilts in each pen and 8 replicate pens per treatment. A control diet based on corn and soybean meal and 8 diets based on corn and a high-protein corn product (48% crude protein) with 2 levels of crystalline L-Val (0 or 0.10%), L-Ile (0 or 0.10%), and L-Trp (0 or 0.05%) were formulated. The crystalline L-Val, L-Ile, and L-Trp increased standardized ileal digestible (SID) Val:Lys from 70 to 80%, SID Ile:Lys from 53 to 63%, and SID Trp:Lys from 18 to 23%, respectively. All diets were formulated to contain 1.00% SID Lys and the 8 diets containing corn protein contained 171% SID Leu:Lys. Individual pig weights were recorded at the beginning and at the conclusion of the 28-d experiment. Data were analyzed using the PROC MIXED of SAS with a pen as the experimental unit. Diet was the fixed effect and block and replicate within block were random effects. Results indicated that final body weight and average daily gain were not different between pigs fed the control diet and pigs fed the diet with Val and Trp addition, but greater (P < 0.001) than for pigs fed the diet with Val addition, Ile addition, Trp addition, Val and Ile addition, Ile and Trp addition, or Val, Ile, and Trp addition (Table 1). In conclusion, addition of Val and Trp to diets with excess Leu may prevent negative effects of excess Leu in diets for growing pigs.

305 Methane Emissions and Apparent Total Tract Nutrient Digestibility of Feedlot Beef Steers Under Intensive Management with or Without an Added Nutritional Packet

2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 166-167
Author(s):  
Andrea M Osorio ◽  
Kaue T Tonelli Nardi ◽  
Igor Gomes Fávero ◽  
Kaliu G Scaranto Silva ◽  
Kymberly D Coello ◽  
...  
Keyword(s):  
Treatment Period ◽  
Repeated Measures ◽  
Fixed Effects ◽  
Block Design ◽  
Random Effect ◽  
Methane Emissions ◽  
Nutrient Digestibility ◽  
Experimental Unit ◽  
Beef Steers ◽  
Ch4 Production

Abstract The effects of a nutritional packet were evaluated on CH4 emissions and apparent total tract nutrient digestibility of feedlot beef steers. Thirty Angus-crossbred steers (BW = 542 ± 8.4 kg) were used in a randomized complete block design and allocated into pens equipped with SmartFeed (C-Lock; 15 steers/treatment). Steers were consuming a steam-flaked corn-based diet (88% concentrate DM basis) ad libitum for the last 65 d on feed, and received the following treatments: 1) control and 2) a nutritional packet [0.29% DM basis; live yeast (Saccharomyces cerevisiae; 8.7 Log CFU/g); Vitamin C (5.4 g/kg); Vitamin B1 (13.33 g/kg); NaCl (80 g/kg); KCl (80 g/kg)]. Methane emissions and apparent total tract nutrient digestibility were measured during 3 periods with 5-d of collections each. Gas emissions from steers were measured utilizing the SF6 tracer technique. Feed and fecal samples were collected once and twice (0700 h and 1600 h) daily, respectively, to determine digestibility of nutrients using iNDF as an internal marker. Steer was considered the experimental unit. Data were analyzed as repeated measures using the MIXED procedure of SAS with the fixed effects of treatment, period, and their interaction, and the random effect of block. No treatment × period interactions (P ≥ 0.125) were observed for DMI and any of the CH4 production variables (g/day, g/kg BW0.75, g/nutrient intake, and g/nutrient digested). Moreover, treatments did not affect digestibility of DM, OM, or ADF (P ≥ 0.300); however, digestibility of NDF was increased for treated cattle (P = 0.013), which resulted in a tendency (P = 0.098) to decrease CH4 production in g per kg NDF intake and decreased (P = 0.020) grams CH4 per kg NDF digested. The nutritional packet may be altering ruminal fermentation on intensively managed steers and improving fiber digestibility, which can have benefits on CH4 emission intensity.

scholarly journals The microbial community of the gut differs between piglets fed sow milk, milk replacer or bovine colostrum

2017 ◽  
Vol 117 (7) ◽  
pp. 964-978 ◽  
Author(s):  
Ann-Sofie R. Poulsen ◽  
Nadieh de Jonge ◽  
Sugiharto Sugiharto ◽  
Jeppe L. Nielsen ◽  
Charlotte Lauridsen ◽  
...  
Keyword(s):  
Gut Microbiota ◽  
Amplicon Sequencing ◽  
Bovine Colostrum ◽  
Milk Replacer ◽  
Rrna Gene ◽  
Bacterial Dna ◽  
Faecal Samples ◽  
Milk Replacers ◽  
The 16S Rrna Gene ◽  
Gut Microbiota Composition

AbstractThe aim of this study was to characterise the gut microbiota composition of piglets fed bovine colostrum (BC), milk replacer (MR) or sow milk (SM) in the post-weaning period. Piglets (n36), 23-d old, were randomly allocated to the three diets. Faecal samples were collected at 23, 25, 27 and 30 d of age. Digesta from the stomach, ileum, caecum and mid-colon was collected at 30 d of age. Bacterial DNA from all samples was subjected to amplicon sequencing of the 16S rRNA gene. Bacterial enumerations by culture and SCFA analysis were conducted as well. BC-piglets had the highest abundance ofLactococcusin the stomach (P<0·0001) and ileal (P<0·0001) digesta, whereas SM-piglets had the highest abundance ofLactobacillusin the stomach digesta (P<0·0001). MR-piglets had a high abundance of Enterobacteriaceae in the ileal digesta (P<0·0001) and a higher number of haemolytic bacteria in ileal (P=0·0002) and mid-colon (P=0·001) digesta than SM-piglets. BC-piglets showed the highest colonic concentration of iso-butyric and iso-valeric acid (P=0·02). Sequencing and culture showed that MR-piglets were colonised by a higher number of Enterobacteriaceae, whereas the gut microbiota of BC-piglets was characterised by a change in lactic acid bacteria genera when compared with SM-piglets. We conclude that especially the ileal microbiota of BC-piglets had a closer resemblance to that of SM-piglets in regard to the abundance of potential enteric pathogens than did MR-piglets. The results indicate that BC may be a useful substitute for regular milk replacers, and as a feeding supplement in the immediate post-weaning period.

scholarly journals Evaluation of different corn textures in dry grain or silage forms for piglets from 7 to 15 kg

2009 ◽  
Vol 38 (9) ◽  
pp. 1757-1761
Author(s):  
José Vieira Neto ◽  
Vinícius de Souza Cantarelli ◽  
Elias Tadeu Fialho ◽  
Márcio Gilberto Zangeronimo ◽  
Nikolas de Oliveira Amaral ◽  
...  
Keyword(s):  
Dry Matter ◽  
Block Design ◽  
Metabolizable Energy ◽  
Experimental Unit ◽  
Feed Conversion ◽  
Initial Weight ◽  
Large White ◽  
Randomized Block Design ◽  
Independent Form ◽  
Ensilage Corn

The objective of this study was to evaluate different textures and storage forms of corn in piglet diets. Two experiments were conducted, one of which tested digestibility of nutrients and the other diet performance. In the digestibility trial, 24 crossbred (Landrace X Large White) barrows with an initial weight of 18.7 ± 1.5 kg were used. They were kept in metabolic cages in a randomized block design using the factorial arrangement 2 X 2 (type of corn - dent and flint X type of storage - moist grain silage and dry grain) and six replicates, with one animal as the experimental unit. The values of metabolizable energy (ME) obtained were 3841 kcal of ME/kg of dry matter (DM) from dry corn, 3912 kcal of ME/kg of DM from moist corn, 4022 kcal of DM from ensiled dry corn and 3928 kcal of ME/kg of DM from ensiled humidity corn. The ensilage process increases the digestibility coefficient of dry matter and crude protein and increases the digestible energy of the diets, independent of the process form. The type of corn did not influence these variables. In the performance trial, 60 barrows (initial weight of 6.5 ± 1.5 kg) of the same stock were used during 28 days of post-weaning distributed in the same experimental design, with five replicates and three animals as the experimental unit. The treatments did not influence the daily weigh gain or the daily feed intake. The dentate corn and the ensilage process decreased the feed conversion of the independent form (no significant interaction). The substitution of dry for ensilage corn increased the digestibility of the nutrients in the diets and the feed conversion of the piglets from 7 to 15 kg. Dentate corn promotes best feed conversion in this phase.

scholarly journals Effect of diets with different levels of guanidinoacetic acid on newly weaned piglets

2017 ◽  
Vol 38 (6) ◽  
pp. 3887 ◽  
Author(s):  
Karla Andrade Teixeira ◽  
Alessandra Gimenez Mascarenhas ◽  
Heloisa Helena de Carvalho Mello ◽  
Emmanuel Arnhold ◽  
Patrícia Da Silva Assunção ◽  
...  
Keyword(s):  
Creatine Kinase ◽  
Block Design ◽  
Initial Period ◽  
Daily Weight Gain ◽  
Experimental Unit ◽  
Feed Conversion ◽  
Guanidinoacetic Acid ◽  
Significant Difference ◽  
Increasing Trend ◽  
Blood Creatinine

Ninety barrow piglets weaned at 21 days old were used to evaluate the effects of guanidinoacetic acid (GAA) supplementation on their performance and blood creatinine and creatine kinase (CK) levels during the nursery phase (21 to 63 days old). The piglets were distributed in a randomized block design with five treatments (0, 0.05, 0.10, 0.15, and 0.20% GAA inclusion), six repetitions, and three animals per repetition. The experimental rations were formulated to meet the nutritional requirements for the phases of 21 to 32, 33 to 42 and 43 to 63 days of age. The experimental diets and water were offered ad libitum throughout the experimental period. At 42 and 63 days old, blood was collected from one animal in each experimental unit for creatinine and creatine kinase analysis. The analysed variables were daily weight gain (DFG), daily feed intake (DFI), and feed conversion (FC) in the 21 to 42 days old and 21 to 63 days old of the nursery phase. All variables were subjected to analysis of variance and regression analysis. We adopted ? = 0.05, and considered a trend to be present when ? was between 0.05 and 0.10. In the pre-initial period (21 to 42 days old), there was an increasing trend in daily weight (P = 0.069), which increased to the level of 0.97% of GAA inclusion. The DFI and FC were not significantly affected by the addition of different GAA levels in the diet. Creatinine levels in the blood at 42 and 63 days old were not significantly influenced by the treatments. The level of CK at 42 days old showed an increasing trend (P = 0.077) that occurred quadratically to the level of 0.085% of GAA inclusion, with no significant difference for this variable at 63 days old. The use of GAA did not promote improvements in performance or blood creatinine and CK levels in piglets in the nursery phase.

scholarly journals Performance and intestinal development of chicks submitted to different rations and different types of incubation

2021 ◽  
Vol 15 (2) ◽  
pp. 154-161
Author(s):  
Eliete Souza Santana ◽  
José Neto Cassiano de Camargo ◽  
Michele Laboissière ◽  
Roberto Moraes Jardim Filho
Keyword(s):  
Weight Gain ◽  
Feed Intake ◽  
Block Design ◽  
Phase 1 ◽  
Live Weight ◽  
Experimental Unit ◽  
Average Weight ◽  
Intestinal Development ◽  
Feed Conversion ◽  
Feed Processing

The objective of this work was to evaluate the effects of two types of incubation and two types of feeding on the performance of chickens and intestinal development of chicks during the pre-starter phase (1 to 7 days of age). One-year-old male Ross® chicks were used. The experimental design was a randomized complete block design, in a 2x2 factorial design, with two types of feed processing (bran and micropelleted) and two types of incubation machines (single and multiple stage), totaling four treatments, with four treatment repetitions each. Twelve birds per experimental unit were used, totaling 192 animals. Performance evaluations were conducted, such as mean weight, weight gain, feed intake, feed conversion, bowel weight and size, and percentage of intestine in relation to live weight. Histomorphometric tests on the height of the villi and depth of the duodenal crypts were also performed. The two incubation machines did not influence any of the analyzed variables. Even though both had the same composition, the micropelleted feed resulted in positive gains for the performance variables: average weight, weight gain, feed intake, and feed conversion when compared to the meal, but was unable to significantly influence the morphometric measurements of the duodenum of chicks in the pre-starter phase.

scholarly journals Effect of farrowing pen size on pre-weaning performance of piglets

2021 ◽  
Author(s):  
Katherine D Vande Pol ◽  
Austin L Laudwig ◽  
Aaron M Gaines ◽  
Beau A Peterson ◽  
Caleb M Shull ◽  
...  
Keyword(s):  
Litter Size ◽  
Block Design ◽  
Commercial Production ◽  
Experimental Unit ◽  
Pig Production ◽  
Time Period ◽  
Randomized Complete Block Design ◽  
The Impact ◽  
Cross Fostering ◽  
Size At Birth

Abstract Litter sizes in commercial pig production have increased substantially over recent years; however, farrowing pen sizes have generally not changed over the same time period. The objective of this study was to evaluate the effect of farrowing pen size on piglet pre-weaning growth and mortality. Differences in pen size were created by varying the width of pens of the same length, increasing the creep area available to the piglets. The study used a total of 1,786 litters in a randomized complete block design to compare two Farrowing Pen Size treatments (FPS): Standard (pen width 1.52 m) and Increased (pen width 1.68 m). Litter sizes were equalized across treatments (12.9 ± 1.95 piglets) at 24 h after birth using cross-fostering. Litter weights were collected at birth and weaning (21.3 ± 2.08 d); pre-weaning mortality was recorded. The experimental unit was the litter; models included FPS and replicate. Farrowing Pen Size had no effect (P &gt; 0.05) on litter size at birth (12.8 and 13.0 for the Standard and Increased FPS, respectively), after cross-fostering (12.9 for both treatments), or at weaning (11.2 and 11.3, respectively). There was no effect (P &gt; 0.05) of FPS on total litter or average piglet weight at birth, after cross-fostering, and at weaning. These results suggest no benefit in piglet performance from increasing the width of farrowing pens. As litter sizes continue to increase in commercial production, further research is warranted to re-evaluate the impact of farrowing pen size on pre-weaning mortality.

scholarly journals Physiological responses of beet plants irrigated with saline water and silicon application

2020 ◽  
Vol 11 ◽  
pp. E3113
Author(s):  
José Sebastião de Melo Filho ◽  
Toshik Iarley da Silva ◽  
Anderson Carlos de Melo Gonçalves ◽  
Leonardo Vieira de Sousa ◽  
Mario Leno Martins Véras ◽  
...  
Keyword(s):  
Stomatal Conductance ◽  
Gas Exchange ◽  
Carbon Concentration ◽  
Saline Water ◽  
Block Design ◽  
Essential Element ◽  
Net Photosynthesis ◽  
Crop Productivity ◽  
Experimental Unit ◽  
Randomized Block Design

Although not considered an essential element, silicon can be used to increase crop productivity, especially under stress conditions. In this sense, the objective was to evaluate the gas exchange of beet plants irrigated with saline water depending on the application of silicon. The experiment was conducted in a randomized block design, in a 5 x 5 factorial, referring to five levels of electrical conductivity of irrigation water (ECw): (0.5; 1.3; 3.25; 5.2 and 6.0 dS m-1) and five doses of silicon (0.00; 2.64; 9.08; 15.52 and 18.16 mL L-1), with six beet plants as an experimental unit. The effect of treatments on beet culture was evaluated at 30 and 60 days after irrigation with saline water from measurements of internal carbon concentration, stomatal conductance, net photosynthesis rate, instantaneous water use efficiency and instantaneous carboxylation efficiency using the LCpro+Sistem infrared gas analyzer (IRGA). Irrigation with saline water reduced the gas exchange of beet plants at 60 days after irrigation, but at 30 days after irrigation, the use of saline water increased stomatal conductance, transpiration rate and internal carbon concentration. The application of silicon decreased stomatal conductance, internal carbon concentration and efficiency in the use of water, but increased the rate of net photosynthesis, the rate of transpiration and instantaneous efficiency of carboxylation at 30 and 60 days after irrigation.

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