scholarly journals Analysis of the area and the number of pulmonary alveoli through the normal aging process in CD1 mouse

2020 ◽  
Author(s):  
Marta Ortega-Martínez ◽  
Esthefania Gutiérrez-Arenas ◽  
Vanessa Gutiérrez-Dávila ◽  
Alberto Niderhauser-García ◽  
Ricardo M Cerda-Flores ◽  
...  

During the aging process, the lung exhibits structural changes accompanied by a decline in its function. The related information currently available is still scarce and contradictory. In addition, changes in some pulmonary parameters through aging process are species- and strain- dependent. The aim of this study was the assessment of the area and the number of pulmonary alveoli through the normal aging process in CD1 mouse. Paraffin-embedded sections of lungs from CD1 mice at age of 2, 6, 12, 18, or 24 months were stained with hematoxylin and eosin and examined using a light microscope. Images were captured using a camera linked to an image analysis software to measure areas and count alveoli. There was a significant difference in the alveolar area among the ages analyzed (F=87.53, Sig.=0.000). The alveolar area of the 6-, 12-, 18-, and 24-month-old mice was significantly greater (all p values < 0.001) than in mice at 2 months of age. Also, the alveolar number was significantly different among the ages tested (F=3.21, Sig.=0.023). The number of alveoli in mice at 2 months of age was greater than in mice at all other age groups, reaching statistical significance when compared with the 6-, 12-, and 18-month-old mice ( p values of 0.044, 0.014, and 0.002, respectively). Thus, we observed an increase in alveolar area and a decrease in alveolar number through the aging process. This information might be useful to understand pathologic changes underlying susceptibility of elderly individuals to chronic lower respiratory tract diseases.

2008 ◽  
Vol 294 (4) ◽  
pp. H1562-H1570 ◽  
Author(s):  
Hélène Bulckaen ◽  
Gaétan Prévost ◽  
Eric Boulanger ◽  
Géraldine Robitaille ◽  
Valérie Roquet ◽  
...  

The age-related impairment of endothelium-dependent vasodilatation contributes to increased cardiovascular risk in the elderly. For primary and secondary prevention, aspirin can reduce the incidence of cardiovascular events in this patient population. The present work evaluated the effect of low-dose aspirin on age-related endothelial dysfunction in C57B/J6 aging mice and investigated its protective antioxidative effect. Age-related endothelial dysfunction was assessed by the response to acetylcholine of phenylephrine-induced precontracted aortic segments isolated from 12-, 36-, 60-, and 84-wk-old mice. The effect of low-dose aspirin was examined in mice presenting a decrease in endothelial-dependent relaxation (EDR). The effects of age and aspirin treatment on structural changes were determined in mouse aortic sections. The effect of aspirin on the oxidative stress markers malondialdehyde and 8-hydroxy-2′-deoxyguanosine (8-OhdG) was also quantified. Compared with that of 12-wk-old mice, the EDR was significantly reduced in 60- and 84-wk-old mice ( P < 0.05); 68-wk-old mice treated with aspirin displayed a higher EDR compared with control mice of the same age (83.9 ± 4 vs. 66.3 ± 5%; P < 0.05). Aspirin treatment decreased 8-OHdG levels ( P < 0.05), but no significant effect on intima/media thickness ratio was observed. The protective effect of aspirin was not observed when treatment was initiated in older mice (96 wk of age). It was found that low-dose aspirin is able to prevent age-related endothelial dysfunction in aging mice. However, the absence of this effect in the older age groups demonstrates that treatment should be initiated early on. The underlying mechanism may involve the protective effect of aspirin against oxidative stress.


2014 ◽  
Vol 6 (3) ◽  
pp. 49-57 ◽  
Author(s):  
Fiekumo Igbida Buseri ◽  
Charity Ngozi Okonkwo

Background: This study aims at investigating the seroprevalence of HIV infection among status naive pregnant women and probable vertical transmission in Sokoto, Nigeria.Materials and Methods: This cross-sectional study examined 13,026 apparently healthy pregnant women aged between 14 and 45 years and 312 mother-baby pairs in 4 different hospital settings in Sokoto State, North West, Nigeria between March, 2011 and February, 2013. The babies were aged between 8 and 16 months. HIV screening was performed using qualitative rapid tests and ELISA and HIV-DNA polymerase chain reaction (PCR) techniques. Measurement of CD4+ T-lymphocytes was carried out by the BD FACScount System. All seropositive pregnant women were immediately placed on triple antiretroviral therapy (ART) throughout the duration of the pregnancy and beyond.Results: An overall 2.4% prevalence of HIV-1 infection among the pregnant women and 20.5% incident of mother-to-child transmission were found. Of the seropositive pregnant women, 75.0% were full-time house wives, 13.8% and 11.2% were traders and civil servants respectively; of which, 70.2% were within the ages of 14 and 27 years (youthful predominance). Pearson’s χ2analysis did not show any statistically significant difference in the Mean values in the 4 health facilities (χ2 =2.084, df=3, P-value=0.555). Similarly, no significant difference in HIV seropositivity in the demographic data of the pregnant women were observed (P>0.05). Infection was recorded in all age groups but there was no statistical significance between age groups and infection (P = 0.833). Of the 64 seropositive babies, 62 (92.5%) contracted HIV from antiretroviral therapy non-adherence mothers (χ2 =271.457, df=1, P<0.01), OR=1506.6 (95%CI=285.5-7950.4). Conclusion: This study found high prevalence of vertical transmission due to ART non-adherence. Intervention initiatives should, therefore, focus seriously on ART non-adherence. DOI: http://dx.doi.org/10.3126/ajms.v6i3.11530Asian Journal of Medical Sciences Vol.6(3) 2015 49-57  


Author(s):  
Babak Alikhani ◽  
Bennet Hensen ◽  
Arne Grosser ◽  
Maria Inés Cartes Febrero ◽  
Markus von Bestenbostel ◽  
...  

Purpose To assess digital patient briefing as an alternative to conventional paper documentation. Materials and Methods 502 patients with a planned computed tomography (CT) examination were selected for digital patient briefing using the E-ConsentPro software from Thieme Compliance on an iPad by Apple (Cupertino, California, USA). For the analysis, three age groups were formed. The time required for the patient briefing, the number of open questions as well as the time needed for discussion with physicians were determined. Student’s t-test was performed to assess statistical significance. Results There was no significant difference between patient age and briefing time which was about 20 minutes on average. The number of open or unclear questions increased with patient age. While patients younger than 30 years of age had about 2 open questions, patients over 30 and 60 years had about 4 and 5 questions, respectively. The total time needed for discussion with physicians was less than 2 minutes on average. A significant difference in the time required for discussion with physicians could not be observed between the individual age groups. Conclusion Tablet-based digital briefing allows the storage of patient documents with reasonable time and effort. Furthermore, it minimizes the risk of data loss. Key Points  Citation Format


2019 ◽  
Vol 35 (6) ◽  
Author(s):  
Uzma Bashir ◽  
Moizza Tahir ◽  
Muhammad Irfan Anwar ◽  
Faisal Manzoor

Background & Objective: Cutaneous leishmaniasis (CL) is endemic in developing countries like Pakistan. Pentavalent antimonials are still drug of choice, despite being toxic and intolerable for patients. Second line treatments have been extensively studied but the results of their efficacy are conflicting. This, to our knowledge, will be the first study in this regard. Our objective was to  determine if combination of oral itraconazole with intralesional (IL) meglumine antimoniate (MA) reduces the duration of treatment for cutaneous leishmaniasis, as compared to intralesional MA alone. Methods: A randomized controlled trial (single blinded) was carried out from August 2017 till December 2017 on 69 patients who fulfilled inclusion criteria. They were assigned to Group-A or B by lottery method. Group-A patients received IL MA once a week while Group-B received oral itraconazole 200mg, once daily, for six weeks along with similar regimen of IL MA as Group-A. The patients were assessed every three weeks by the blinded assessor till clinical cure was achieved. A follow up visit, two months after clinical cure was done to look for relapse of the disease. Results: Thirty patients in Group-A and 35 patients in Group-B completed the study. At 3, 6, 9 and 12 weeks the patients were assessed for: no, partial or complete response and results of the two groups were compared for statistical significance. The p-values of 0.20, 0.57 and 0.11 at 3, 6 and 9 weeks, respectively, depict that there was no significant difference at any step of assessment between the two groups in terms of healing. The p values of each t test was >0.05 refuting the hypothesis. Conclusion: Combination of oral itraconazole with intralesional MA offered no benefit over intralesional MA alone in the management of cutaneous leishmaniasis in terms of duration of therapy.  Abbreviations Used: IL = Intralesional,  MA = Meglumine Antimoniate, LD = Leishmania Donovan, CL = Cutaneous leishmaniasis, CMH = Combined Military Hospital. doi: https://doi.org/10.12669/pjms.35.6.363 How to cite this:Bashir U, Tahir M, Anwar MI, Manzoor F. Comparison of Intralesional Meglumine Antimonite along with oral Itraconazole to Intralesional Meglumine Antimonite in the treatment of Cutaneous Leishmaniasis. Pak J Med Sci. 2019;35(6):1669-1673.   doi: https://doi.org/10.12669/pjms.35.6.363 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


2021 ◽  
Vol 15 (10) ◽  
pp. 3117-3121
Author(s):  
Bandar Al- Abdulwahhab ◽  
Ali Barakat ◽  
Pradeep Koppolu ◽  
Abdulrahman Al- Saffan ◽  
Mada Al- Qahtani ◽  
...  

Esthetic is considered a prime concern for both the practitioners and patients in the area of dentistry. Dentists should thoroughly understand the factors like beauty, harmony, balance, and proportion as are they perceived by the societies while planning treatment. Aim: The aim of the study is to evaluate how different gender, age groups, marital status, educational level, and previous cosmetic dental experience of the Saudi dental professionals and the laypersons will influence the selection of an ideal veneer smile design with various shapes of anterior maxillary teeth to their esthetic preferences. Methods: The present survey-based study included 282 dental professionals and lay persons as the sample. The patient and the treating doctor were administered the questionnaire and were further asked to rank the images in terms of score and order of preference. They were asked to put a checkmark on the five cells visual analogue scale (VAS) next to each image to define attractiveness, considering that, on the VAS, one indicated the least attractive and 5 showed the most attractive veneer smile design. Results: Out of 282 participants 106 were male and were 176 female participants. The participant ages were between 16 and 53 years. To find the association between categorical variables, the Mann-Whitney U test was used (with statistical significance set at 0.05). When the responses were compared between the dentist and non-dentist, there was only a statistically significant difference (0.013) for picture 11 among all the 12 pictures responses. Conclusion: The Dental professionals and laypersons showed more similarities than differences when esthetic dental values were considered. Dental professionals were more critical than laypersons. Keywords: Veneer, Dentists, Smile


Blood ◽  
2006 ◽  
Vol 108 (11) ◽  
pp. 4117-4117
Author(s):  
Roger C. Munro ◽  
Lisa J. Wakeman ◽  
Saad Al-Ismail

Abstract Introduction: There is published evidence which indicates that advancing age may be associated with higher plasma concentrations of fibrinogen. There is also evidence that derived fibrinogen values are significantly higher than Clauss measurements and that this discrepancy is greater in patients receiving warfarin. The purpose of this study was to determine whether age related derived fibrinogen levels are similar in both warfarin and non-warfarin groups. Methods: Venous samples were collected into siliconised glass B-D Vacutainers containing tri-sodium citrate (Ref: 367691) from 1000 patients receiving long term warfarin treatment and an equal number of age-matched patients not receiving warfarin. Genders were equally represented in both groups. Patients in both groups were categorized into 5 years age bands as follows: &lt;40 n=23: 40–44 n=20: 45–49 n=43; 50–54 n=74: 55–59 n=113: 60–64 n=155: 65–69 n=178: 70–74 n=191: 75–79 n=124; 80–84 n=56: 85–89 n=23. Derived fibrinogen was measured in each patient on an ACL300R coagulometer (I L) within 1 hour of collection using IL PT-FIB HS Plus reagent and following the manufacturer’s protocol. Appropriate CLSI guidelines were followed throughout. A normal probability plot of the data was performed to confirm that it did not deviate too much from the normal distribution. Results: The T-test for independent samples using the separate variance estimate showed that there was a statistically significant difference in the mean fibrinogen between patients on warfarin and those not on warfarin (p&lt;0.05) in each group except for the last (85–89 years). There was a statistically significant difference (ANOVA) in the fibrinogen levels of patients of different age in both warfarinised and non-warfarinised groups (p&lt;0.05). The modified least significance procedure in the ANOVA test showed that in the non-warfarin group, most of significant difference in fibrinogen between the different age groups is contributed by the difference between patients under 50 years of age. In the non-warfarin group, it requires an age gap of at least 20 years for the difference in fibrinogen to be statistically significant but in the warfarin group, it only requires an age gap of ten years (p&lt;0.05). Both Linear Regression and Cross Tabulation indicate that the relationship between fibrinogen and age does not vary whether or not the patient is on warfarin. These also show that the effect of age on fibrinogen is not affected by warfarin treatment. Conclusion: Differences or correlations detected in this analysis are of statistical significance but not necessarily clinically significant. Placing age and warfarin treatment in the same model shows that variations in fibrinogen have to be explained by other factors (e.g. technical) not included in the study as only 12% of the error in predicting fibrinogen levels can be reduced by knowing both the age and status of warfarin treatment in individual patients.


2016 ◽  
Vol 04 (02) ◽  
pp. 117-121
Author(s):  
Leena Verma ◽  
Jyoti Gupta ◽  
Sidhi Passi

Abstract Aim: This study was designed to assessthe prevalence of various oral habits among 3-6 years old school childrenin Chandigarh Method: Fifteen different public schools were randomly selected among 45 public schools in Chandigarh area from where 938 children (3-6 yr) were randomly selected (511 boys and 427 girls) with the objectives of recording the prevalence of oral habits. Statistical analysis was carried out using SPSS 10.0 software and the statistical significance of oral habits as related to age groups and sex were calculated by using ANOVA test. Results: The overall prevalence of oral habits in children was 11.7%. Nail biting was the commonest habit (5.5%) followed by thumb sucking (4.05%), tongue thrusting (1.8%), mouth breathing(0.3%) and lip biting(0%). Also, there were no significant differences between boys and girls with respect to prevalence of oral habits. However, a significant difference was observed between different age groups with respect to the prevalence of oral habits. Conclusions: The data revealed that a significant percentage of the children had deleterious oral habits which can contribute towards the development of malocclusion. This helps in identifying children who are in need to intercept the oral habits at earliest to avoid future occurrence of malocclusion.


2019 ◽  
Vol 1 (112) ◽  
pp. 13-18
Author(s):  
Lukas Danilevičius ◽  
Rasa Kreivytė

Background. One of the most important problems for players, coaches and scientists is improving the accuracy of shooting the basket. It is the most important technique for playing basketball, and its dependence on performance is multifaceted. Methods. All 37 games in the 2015 European Championships in U16, U18, U20 and men’s national team were analyzed. Data was selected from the official boxscores of FIBA (International Basketball Federation). The following absolute game-related statistics were gathered: free-throws, 2-point and 3-point field goals (both successful and unsuccessful). The obtained values of the rate of success of 2-point, 3-point and free throw shooting were tested by the non-parametric Mann–Whitney U-test. The statistical analyses were performed using SPSS for Windows, version 20.0, and statistical significance was set at p < .05. Results. Comparing the number and accuracy of shots taken from 2-point distance, we found that there was no significant difference between men of different ages (p > .05). Most 3-point shots were done by U16 youth team, the least was in men’s national basketball team, a significant difference in the indicators (p < .05) was found between all the teams, except between men’s national team and U18 (p > .05). Comparing free-throws’ indicators between different age groups, most throws were done by U16 team, the least were done by the U20 team (p < .05); however, there were no differences between the other significant indicators (p > .05). Comparing free-throws accuracy indicators between the different age groups of basketball teams, significant differences in indicators were found between men’s national basketball team and U20, U16 (p < .05). Conclusions. The count of 2-point shots made in Lithuanian different age basketball national team games was more or less similar. Most 3-point shots were made by U16 team and the national men’s team made least 3-pointers. The indicators of accuracy did not have a significant difference between national teams, except for free throws: men’s national team made these shots most accurately, opposite situation was with U16 team.


2019 ◽  
Vol 7 (6) ◽  
pp. 24 ◽  
Author(s):  
Egemen Ermiş

The purpose of this study is to examine the leadership characteristics of amateur soccer players in terms of positions and some variables. 300 amateur soccer players playing in different clubs in the districts and the city centre of the province of Samsun were included in the study. A personal information form was given to subjects to find out their positions and some variables and Leadership Scale for Sport which was developed by Chelladurai and Saleh (1980) and which included 40 items and five subscales (training and instruction, democratic behaviour, social support, positive feedback and autocratic behaviour) was used to find out leadership levels. SPSS 22.0 program was used in the statistical analysis of the study. When the results of the study were examined, statistically significant difference was found only in positive feedback subscale when the subjects’ leadership levels were compared according to age groups and education levels (p<0.05), while no significance was found in other subscales (p>0.05). When leadership levels were compared in terms of the total period of time they played soccer, their positions, the number of clubs they played for and the state of being the captain, no statistical significance was found in all of the subscales (p>0.05). As a conclusion, it can be seen that leadership levels of soccer players did not differ according to positions, the state of being the captain, the total period of time they played soccer and the number of clubs they played for, while positive feedback was found to develop positively only as age and the state of education increased.


Author(s):  
Patrícia Paixão Fayer ◽  
Maria Marta Conrado ◽  
Luciano Miranda ◽  
Renato Melo Ferreira ◽  
Everton Rocha Soares ◽  
...  

Introdução: Nas últimas décadas aumentou o número de pessoas acima do peso, incluindo crianças e adolescentes, fato que é considerado preocupante, pois pode acarretar diversas doenças crônicas, em longo prazo.Objetivo: Avaliar a prevalência de sobrepeso e obesidade em escolares de um colégio militar, investigando possíveis diferenças entre os sexos e as faixas etárias.Métodos: Estudo quantitativo, transversal, do qual participaram 381 escolares de um colégio militar de Minas Gerais, de 11 a 17 anos, sendo 213 meninos e 168 meninas. Calculou-se o índice de massa corporal (IMC) que foi classificado segundo o PROESP-BR. Para testar diferenças entre os sexos, utilizou-se o teste do Qui-Quadrado (X2) e para testar a relação entre idade e IMC, utilizou-se a correlação de Pearson. Significância estatística: p < 0,05.Resultados: Foi observado sobrepeso em 17,8% (n = 68) e de obesidade em 5,8% (n = 22) dos escolares. Houve correlação positiva entre a idade cronológica e o IMC (r = 0,32; p < 0,0001). Não houve correlação entre estado nutricional e faixa etária. Não foram observadas diferenças significativas entre os sexos, em relação ao sobrepeso e à obesidade (16,9% e 6,6% nos meninos vs. 19,0% e 4,8% nas meninas, respectivamente).Conclusão: A prevalência sobrepeso e obesidade nos escolares do colégio militar, em conjunto, foi de 23,6%, tanto nos meninos quanto nas meninas, sem diferença significativa entre as faixas etárias. Essa prevalência foi similar à média de escolares brasileiros.Prevalence of Overweight and Obesity in Students of a Military SchoolIntroduction: Over the past few decades, the number of overweight people, including children and adolescents has increased significantly, a fact that is considered worrying, as it can lead to several chronic diseases in the long term.Objective: To evaluate the prevalence of overweight and obesity in schoolchildren of a military college, investigating possible differences between sexes and age groups.Methods: A quantitative, cross-sectional study was carried out in which 381 students from a military school in Minas Gerais, aged 11 to 17 years, were 213 boys and 168 girls. The body mass index (BMI) was calculated according to the PROESP-BR. To test for gender differences, the Chi-square test (X2) was used and Pearson's correlation was used to test the relationship between age and BMI. Statistical significance: p <0.05.Results: Overweight was observed in 17.8% (n = 68) and obesity in 5.8% (n = 22) of the students. There was a positive correlation between chronological age and BMI (r = 0.32; p <0.0001). There was no correlation between nutritional status and age range. No significant differences were observed between genders regarding overweight and obesity (16.9% and 6.6% in boys vs. 19.0% and 4.8% in girls, respectively).Conclusion: The prevalence of overweight and obesity in the students of the military college, together, was 23.6% in both boys and girls, with no significant difference between age groups. This prevalence was similar to the average of Brazilian schoolchildren.


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