scholarly journals The Influence of Magnetic Fields on Selected Physiological Parameters of Blood and Tissues in Mice

2018 ◽  
Author(s):  
Hani Abdelsalam ◽  
Mohammed Elywa
Keyword(s):  
Magnetic Fields ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Aminobutyric Acid ◽  
Control Group ◽  
Sod Activity ◽  
Natural Form ◽  
Cell Counts ◽  
Blood Smears ◽  
The Brain

This study aimed to highlight the influence of exposure to different applied magnetic fields (MFs) on SOD, MDA and GSH levels in the liver, LDH and CPK activities in the muscle and γ-aminobutyric acid levels in the brain, as well as some haematological parameters. Adult male albino Swiss mice were divided into 5 equal groups (n = 6), the control group (untreated) and four exposure groups that were exposed to MFs of 20, 40, 60 and 80 Gauss for 5 min/day for 5 days.: Exposure to MFs induced significant decreases in total GSH levels and SOD activity but a significant increase in MDA levels in the liver. By contrast, SMF exposure significantly increased total LDH and total CPK activities in the muscle. The results revealed a significant increase in GABA levels in the brain, as well as decreases in haemoglobin, haematocrit, and red blood cell counts, in addition to platelet counts, after exposure to 20, 40, 60 and 80 Gauss MFs. After exposure to a 40 Gauss MF, the mice showed pathological changes in red blood cells, including changes to the outer membrane of the red blood cells (micronucleus and a serrated edge, with a mild incidence of echinocytes). In the group exposed to a 60 Gauss MF, examination of blood smears clearly showed changes in cell size, with the emergence of abnormal forms, including many areas with no red blood cells (rouleaux formation). With increasing intensity of exposure (80 Gauss), the red blood cells appeared completely different from their natural form and took the form of ovalocytes and bi-micronucleated erythrocytes, which appear in patients with anaemia.: MF exposure caused different metabolic and haematological effects, which appeared to be related to the intensity of SMF exposure. The changes in the biochemical parameters of SMF-exposed mice probably reflect hepatic damage and anaemia caused by kidney failure. Further studies are needed to obtain a better understanding of the effects of MF on biological systems.

scholarly journals Acetylsalicylic acid and morphology of red blood cells

2010 ◽  
Vol 53 (3) ◽  
pp. 575-582 ◽  
Author(s):  
Jacques Natan Grinapel Frydman ◽  
Adenilson de Souza da Fonseca ◽  
Vanessa Câmara da Rocha ◽  
Monica Oliveira Benarroz ◽  
Gabrielle de Souza Rocha ◽  
...  
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Control Group ◽  
Blood Samples ◽  
Morphological Alterations ◽  
Blood Smears ◽  
In Vivo Treatment ◽  
Microscopy Data

This work evaluated the effect of in vitro and in vivo treatment with ASA on the morphology of the red blood cells. Blood samples or Wistar rats were treated with ASA for one hour. Blood samples or animals treated with saline were used as control group. Blood smears were prepared, fixed, stained and the qualitative and quantitative morphology of red blood cells were evaluated under optical microscopy. Data showed that the in vitro treatment for one hour with ASA at higher dose used significantly (p<0.05) modified the perimeter/area ratio of the red blood cells. No morphological alterations were obtained with the in vivo treatment. ASA use at highest doses could interfere on shape of red blood cells.

scholarly journals EXPANDING THE ROLE OF MAGNETIC FIELDS IN RED BLOOD CELLS DEFORMATIONS: DEMONSTRATION OF PARAMAGNETIC AND DIAMAGNETIC FIELDS

2019 ◽  
Vol 7 (2) ◽  
pp. 214-220
Author(s):  
Abraham A. Embi Bs
Keyword(s):  
Magnetic Fields ◽  
Red Blood Cells ◽  
Cross Talk ◽  
Human Blood ◽  
Blood Cells ◽  
Graphite Powder ◽  
Cell Deformation ◽  
Blood Smears

Background: The main purpose of this manuscript is to introduce a biophysics based mechanism of the role of magnetism in temporary cell deformation. Biomagnetism: Biomagnetism has been slowly introduced in the medical literature as follows: The diamagnetic nature of blood reported in1936, the recording of organ emissions from the human body also published in the second half of the last century. It could stated that the same way molecular cross talk influence cells signaling, biomagnetic cross talk could also influence cells functions. Introduction: This manuscript introduces the effect of externally applied magnetic fields (diamagnetic or paramagnetic) causing temporary Red Blood Cells (RBCs) deformations. Results from experiments using diamagnetic material such as human blood with the property of repelling an opposing magnetic field; and paramagnetic material such as carbon based graphite or iron particles are reported. Methods: The work herein presented entailed the trapping (between two similar glass slides) of fresh human blood smear with fine exogenous metal, such as iron filings or graphite powder. The technique requires a standard video microscope and an image recording equipment.   Provocative placement of ferromagnetic or diamagnetic material in in vitro blood smears preparations were found to exhibit temporary in vivo characteristics quoted Temporary In Vivo Blood Smears (TBIS). Established protocol calls for fresh blood smears to be set-aside 5 minutes prior to staining. During the first 2 minutes of the set-aside period, in a typical monolayer smear, one can observe an initial brief time period of approximately 1’ ± 30” of a drying cycle. Usually, a moisture sheen boundary is seen gradually moving from the slide’s edges towards the center. Results: The main findings include a demonstration that both diamagnetic and pararmagnetic material deform RBCs in a variety of shapes. The paramagnetism (attraction) and diamagnetism of graphite powder induced temporary RBCs deformation in the form of teardrops. For the first time, an ongoing cellular deformation process was captured in video recordings. Conclusions: When in vivo cells in blood smears, such as RBCs, are fronted by exogenous magnetic fields, temporary cell deformation occurs.

scholarly journals Babesia microti Primarily Invades Mature Erythrocytes in Mice

2006 ◽  
Vol 74 (6) ◽  
pp. 3204-3212 ◽  
Author(s):  
Ingo Borggraefe ◽  
Jie Yuan ◽  
Sam R. Telford ◽  
Sanjay Menon ◽  
Rouette Hunter ◽  
...  
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Inbred Mouse ◽  
Flow Cytometric Analysis ◽  
Babesia Microti ◽  
Mouse Strains ◽  
Strongly Correlated ◽  
Mature Erythrocyte ◽  
Blood Smears ◽  
Nucleic Acid Stain

ABSTRACT Babesia microti is a tick-borne red blood cell parasite that causes babesiosis in people. Its most common vertebrate reservoir is the white-footed mouse. To determine whether B. microti invades reticulocytes, as does the canine pathogen B. gibsoni, we infected the susceptible inbred mouse strains C.B-17.scid and DBA/2 with a clinical isolate of B. microti. Staining of fixed permeabilized red blood cells with 4′,6′-diamidino-2-phenylindole or YOYO-1, a sensitive nucleic acid stain, revealed parasite nuclei as large bright dots. Flow cytometric analysis indicated that parasite DNA is primarily found in mature erythrocytes that expressed Babesia antigens but not the transferrin receptor CD71. In contrast, CD71-positive reticulocytes rarely contained Babesia nuclei and failed to express Babesia antigens. Accordingly, the frequency of YOYO-1-positive, CD71-negative cells strongly correlated with parasitemia, defined as the frequency of infected red blood cells assessed on Giemsa-stained blood smears. Importantly, the absolute numbers generated by the two techniques were similar. Parasitemia was modest and transient in DBA/2 mice but intense and sustained in C.B-17.scid mice. In both strains, parasitemia preceded reticulocytosis, but reticulocytes remained refractory to B. microti. In immunocompetent C.B-17 mice, reticulocytosis developed early, despite a marginal and short-lived parasitemia. Likewise, an early reticulocytosis developed in resistant BALB/cBy and B10.D2 mice. These studies establish that B. microti has a tropism for mature erythrocytes. Although reticulocytes are rarely infected, the delayed reticulocytosis in susceptible strains may result from parasite or host activities to limit renewal of the mature erythrocyte pool, thereby preventing an overwhelming parasitemia.

scholarly journals BIOLOGICAL EFFECTS OF SILICON OXIDE NANOPARTICLES ON BROILER CHICKEN

2020 ◽  
Vol 5 (2) ◽  
pp. 63-71
Author(s):  
Vladimir Nikulin ◽  
Aleksandra Mustafina
Keyword(s):  
Red Blood Cells ◽  
Total Protein ◽  
Blood Cells ◽  
Broiler Chickens ◽  
Broiler Chicken ◽  
Silicon Oxide ◽  
The Body ◽  
Control Group ◽  
The Third ◽  
Experimental Group

The aim of the study is to increase the productive qualities of broiler chickens by including ultrafine silicon oxide into main diet. During the experiment, the biological effect of ultrafine silicon oxide on broiler chickens was estab-lished. Use of ultrafine SiO2 particles for poultry feeding contributed to an increase in the number of red blood cells and content of total protein and albumins. By the end of the experiment, the number of red blood cells in birds in-creased by 17.43% (P≤0.001) – in the blood of birds of the first experimental group, 16.51% (P≤0.01) – the second one, 20.80% (P≤0.001) – the third experimental and 21.71% (P≤0.001) – the fourth experimental group, compared with the indicator of the control group. The amount of total protein in blood serum of chickens of the first and the second experimental groups increased by 1.36-1.39 %, in the third and fourth ones there was a significant (P≤0.05) increase by 5.45 and 3.05%, respectively. The blood glucose content of chickens in the experimental groups is higher by 8.04-23.65% compared to this indicator with ones in the control group. During the experiment feed con-sumption per 1 kg gain of live weight decreased: in the first experimental group by 3.00 % in the second by – 0.50 %, the third – 6.00 % the fourth– by 4.50 %, compared to this with the control group. The chicken’s vibrancy of the first the experimental group was higher by 4.77 %, the second – by 6.20 %, the third – by 19.25 % and the fourth– by 11.59% than in the control one. Consequently, when converting the feed energy into the body energy of a broiler chicken, the energy conversion coefficient of the experimental group of poultry is higher than that of the control one by 7.16-21.76 %. Thus, the most optimal dose for further research was determined.

scholarly journals The Effect of Experimental Escherichia coli Infection on Some Blood Parameters and Histological Changes in Male Rats

2011 ◽  
Vol 35 (2) ◽  
pp. 22-27
Author(s):  
Hayder AL – Zamely
Keyword(s):  
Escherichia Coli ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Histopathological Examination ◽  
Hemoglobin Concentration ◽  
Blood Parameters ◽  
Infected Group ◽  
Male Rats ◽  
Control Group ◽  
Renal Tubules

This study was carried out to investigate the effect of experimental infection by Escherichia coli on some blood parameters and histopathological changes in male rats to reach this objective twenty male rats (Rattus norvigicus) were divided into two groups, housed under same conditions of water, feed, temperature, light and humidity. The first one served as control group, while the second was experimentally infected with Ecoli by administration of 2ml / rat of (1×109) colony forming unit/ml of E-coli. Five days later blood were obtained from animals by cardiac puncture to measure the total and differential white blood cells count hemoglobin concentration packed cells volume red blood cells count, and thrombocytes count. One animal from each group was killed and histopathological examination was done for intestine kidney liver and spleen to detect the effect of infection on these organs. The results revealed a significant increase in total leukocytes count (P<005) in infected group while the differential count showed a significant increase in neutrophils and significant decrease in lymphocytes (P< 0.05) in same group. There were a significant decrease in hemoglobin concentration packed cell volume and non significant decrease in red blood cells count. While thrombocytes count showed a significant decrease in infected group compared with control group (P<0.05). The histopathological examination of infected male rats intestine was demonstrated mild diffuse inflammation which include increased numbers of neutrophile in tissue intraepithelial lymphocytes and goblet cells In kidney renal tubules showed extensive epithelial swelling with decreased lumen space and generalized necrotic changes with interstitial hemorrhage in renal cortex. In liver hepatocytes were swollen with decreased sinusoidal spaces and widely distributed necrotic foci were seen. There is extensive splenic necrosis with infiltration of leukocytes from white pulp to red pulp of spleen.

scholarly journals The mechanism of adaptation of the organism of patients with chronic heart failure combined with vitamin D deficiency and the morphofunctional state of peripheral blood erythrocytes

2019 ◽  
Vol 10 (3) ◽  
pp. 352-357
Author(s):  
N. I. Baryla ◽  
I. P. Vakaliuk ◽  
S. L. Pоpеl’
Keyword(s):  
Heart Failure ◽  
Vitamin D ◽  
Chronic Heart Failure ◽  
Red Blood Cells ◽  
Vitamin D Deficiency ◽  
Peripheral Blood ◽  
Blood Cells ◽  
Functional Class ◽  
Exercise Stress ◽  
Control Group

The problem of structural changes in peripheral blood erythrocytes in patients with chronic heart failure in combination with vitamin D deficiency during exercise stress remains insufficiently studied. Vitamin receptors are located on smooth myocytes, endothelial cells, cardiomyocytes and blood cells. It affects the state of the cell membrane, the contractile function of the myocardium, the regulation of blood pressure, cardiac remodeling and reduction of left ventricular hypertrophy. Therefore, it is important to assess the level of vitamin D in blood plasma in individuals with chronic heart failure and to identify the effect of its deficiency on the state of peripheral red blood cells when performing a 6-minute walk test. A total of 75 patients of the main group with chronic heart failure stage II A, I–II functional class with different levels of vitamin D deficiency were examined. The control group included 25 patients with chronic heart failure stage II A, functional class I–II without signs of vitamin D deficiency. The average age of patients was 57.5 ± 7.5 years. All patients were asked to undergo the 6 minutes walking test. The level of total vitamin D in plasma was determined by enzyme immunoassay. Morphological studies of erythrocytes were performed on the light-optical and electron-microscopic level. The obtained results showed that patients of the main group with chronic heart failure had a decrease in vitamin D by 2.2 times compared with the control group. Correlation analysis showed a directly proportional relationship between vitamin D deficiency and the number of red blood cells of a modified form and red blood cells with low osmotic resistance. Dosed exercise stress in patients with chronic heart failure against a background of vitamin D deficiency leads to an increase in the number of reversibly and irreversibly deformed erythrocytes and a decrease in their osmotic stability. This indicates a disorder in the structural integrity of their membrane and can have negative consequences for the somatic health of such patients.

Effect Of Citrullus Lanatus Juice On Hemoglobin, Red Blood Cells And Liver Enzyme; An Experimental Study

2019 ◽  
Vol 09 (04) ◽  
pp. 257-260
Author(s):  
Shahid Ali ◽  
Nuzhat Sultana ◽  
Muslim Abbas ◽  
Zareen Naz ◽  
Muhammad Akbar Hassan ◽  
...  
Keyword(s):  
Experimental Study ◽  
Red Blood Cells ◽  
Liver Enzyme ◽  
Blood Cells ◽  
Citrullus Lanatus ◽  
Treated Group ◽  
Control Group ◽  
Group A ◽  
Group B ◽  
Post Hoc

Objective: To determine effect of citrullus lanatus juice on hemoglobin and Serum Glutamic Pyruvic Transaminase (SGPT) level. Study design and setting: It was an experimental study conducted on healthy rabbits for sixty days in the research department of pharmacy University of Karachi. Methodology: Current study was planned to evaluate effect of Citrullus lanatus juice on red blood cells, hemoglobin and liver enzyme. 60 days study was performed at 2 different doses i.e 3and 6ml/kg on rabbits. These rabbits were from either gender and were divided into 3 groups their weight ranges from 1200 to 1800 grams. Group A is control group (Normal saline 6ml/kg), Group B is treated group (3 ml/kg), Group C is treated group (6ml/kg). After taking mean of all values they are compared with control group. Significance of mean can be estimated by Tukes Post Hoc Test. P<0.05 estimated as significant. Results: It was found that count of red blood cells rises significantly along with rise in hemoglobin level. As far as liver enzyme serum glutamate pyruvate transaminase (SGPT) was concerned its concentration decreases slightly. Conclusion: Citrullus lanatus juice contain ingredients which are important for RBC hemoglobin and synthesis .It also contains important antioxidants that have organoprotective role due to which SGPT level decreases even in healthy animals as compared to control groups

Phagocytosis of Foreign Red Blood Cells by the Reticulo-Endothelial System

1957 ◽  
Vol 189 (3) ◽  
pp. 520-526 ◽  
Author(s):  
Bernard N. Halpern ◽  
G. Biozzi ◽  
B. Benacerraf ◽  
C. Stiffel
Keyword(s):  
Endothelial Cells ◽  
Red Blood Cells ◽  
Clearance Rate ◽  
Blood Cells ◽  
Red Cell ◽  
Histological Evidence ◽  
Specific Antibodies ◽  
Cell Counts ◽  
India Ink ◽  
Reticulo Endothelial System

The clearance rate of nucleated pigeon erythrocytes injected intravenously into mice and rats has been calculated either by routine differential red cell counts or by measuring the radioactivity of the erythrocytes tagged with P32. Histological evidence is given that the foreign erythrocytes are phagocytized by the reticulo-endothelial cells of the liver and spleen. The clearance rate of the foreign erythrocytes, which measures the speed of the phagocytosis, follows in mice a regular exponential function similar to this previously established for other colloids. No spontaneous antibodies to pigeon erythrocytes could be detected in mice. The rapid and complex clearance rate of pigeon erythrocytes observed in rats is related to the existence of spontaneous specific antibodies. The simultaneous injection of pigeon erythrocytes and of India ink into mice, both phagocytized by the RE cells, results in a competition between the two substances in favor of the smaller particles of carbon.

A presumptive case of cerebral babesiosis in a dog in Poland caused by a virulent Babesia canis strain

2012 ◽  
Vol 40 (05) ◽  
pp. 367-371
Author(s):  
M Górna ◽  
P. Klimiuk ◽  
M Kalinowski ◽  
S. Winiarczyk ◽  
L. Adaszek
Keyword(s):  
Blood Cells ◽  
Babesia Canis ◽  
Canine Babesiosis ◽  
Neurological Signs ◽  
Tick Activity ◽  
First Case ◽  
Blood Smears ◽  
Pcr Method ◽  
The Brain ◽  
Activity Season

SummaryThe aim of this paper was to present the first case of cerebral canine babesiosis due to infection by Babesia canis in a dog in Poland. A 5-year-old American Staffordshire Terrier was presented with an unusual clinical manifestation of acute babesiosis that included neurological signs and pancytopenia. Despite treatment the dog died. Diagnosis was based on microscopic examination of Giemsa-stained blood smears (detection of piroplasms in red blood cells) and post mortem examination of the brain by histopathology and PCR method. The amplified segment of the Babesia 18S RNA gene was sequenced. This enabled to determine that the cause of the disease had been the strain 18S RNA-B EU622793. This is one of two B. canis strains found endemically in Poland, which reveals a greater virulence than the strain 18S RNA-A EU622792. The described case indicates that this form of canine babesiosis should be taken into account in differential diagnosis in dogs exhibiting neurological symptoms, especially in the tick activity season.

scholarly journals OVALOCYTOSIS ASSOCIATED WITH THE SICKLE CELL TRAIT

Blood ◽  
1949 ◽  
Vol 4 (5) ◽  
pp. 505-510 ◽  
Author(s):  
ROBERT S. FADEM
Keyword(s):  
Blood Cell ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Sickle Cell Trait ◽  
Daily Variation ◽  
Blood Indices ◽  
Sickle Cells ◽  
Cell Counts ◽  
Blood Cell Counts ◽  
Saline Solutions

Abstract 1. A patient has been presented whose circulating red blood cells were composed of 65-84 per cent ovalocytes, 3-11 per cent sickled cells, and some normal appearing discoid cells. 2. The red blood cell counts and the blood indices were within normal limitations. 3. The red blood cells showed an increased resistance to hypotonic saline solutions. See PDF for Table See PDF for Table See PDF for Table 4. The peripheral blood showed a daily variation in the percentage of circulating ovalocytes, from 65 per cent to 84 per cent, and in the percentage of circulating sickle cells, from 3 per cent to 11 per cent. 5. After 72 hours in fresh wet preparations the per cent of ovalocytes remained essentially unchanged from that of fresh fixed blood. 6. The percentage of sickled cells was found to be increased after 18, 24, and 72 hours in fresh wet preparations as compared to the percentage of sickled cells found in fresh fixed preparations. 7. Some of the normal appearing discoid red blood cells were observed to sickle in fresh wet preparations within 18 hours.

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