scholarly journals Novel, economically important semi-dwarf and early mutants: Selection and development from Improved White Ponni Rice (Oryza sativa L.)

2018 ◽  
Author(s):  
S. Ramchander ◽  
Andrew Peter Leon ◽  
Jesima Khan Yasin ◽  
Vinod KK ◽  
Arumugam Pillai
Keyword(s):  
Plant Height ◽  
Oryza Sativa L ◽  
Rice Variety ◽  
Maximum Yield ◽  
Agar Plate ◽  
Chromosome 1 ◽  
Null Alleles ◽  
Single Marker Analysis ◽  
Exon 2 ◽  
Single Marker

Rice variety, Improved White Ponni is a medium duration crop, but highly susceptible to lodging impacting maximum yield losses. The present investigation aimed to identify early and early semi-dwarf mutants in Improved White Ponni by inducing variations using gamma rays without altering its native grain quality traits. Seeds were treated with various doses of after fixation of the LD50 value of gamma radiation and reported that most of the traits exhibit variations in the mutants at various levels of irradiation. The selection for earliness and dwarf plant height was imposed in M2 and it was confirmed by evaluation of M3 generation. Apart from semi-dwarf early mutants, high tillering habit, narrow rolled leaf, upper albino leaf and grassy stunt and extreme dwarf mutants were also identified. Characterization of mutants using already reported genic and linked microsatellite markers associated with semi-dwarfism and earliness resulted that PIC value ranges between 0.037 and 0.949 with an average of 0.382. Single marker analysis revealed that RM302 and RM310 on chromosome 1 and 8 had exhibited an association with the traits plant height, culm and internodal lengths. Of these gene-specific markers, GA20Oxi_1 and GA20Oxi_2 have shown polymorphism among mutants. GA20Oxi_2 showed null alleles in the dwarf mutants and this clearly emphasized that there are some base deletions exists in the region of exon 2 of sd1 region. GA3 response study shown that identified mutants were GA3 responsive except IWP 11-2, IWP 48-2, IWP 50-11 and IWP 33-2 which showed very low responsive. Agar plate assay revealed that, IWP 50-11, IWP 33-2, IWP 43-1, IWP 47-2 and IWP 18-1 had low production of α- amylase. Scanning electron microscope examination on confirmed mutants exhibited larger cell size and a lesser number of cells per unit area than the wild-type which shows that these mutants are defective in GA mediated pathway

Genetic analysis of dormancy and shattering traits in the backcross inbred lines derived from Oryza sativa cv. Swarna / O. nivara Ac. CR100008

2020 ◽  
Vol 57 (1) ◽  
pp. 1-13
Author(s):  
Anusha Galimudi ◽  
Eswari KB ◽  
Lakshmidevi Gandhudi ◽  
Jai Vidhya LRK ◽  
Narender Reddy S ◽  
...  
Keyword(s):  
Oryza Sativa ◽  
Inbred Lines ◽  
Chromosome 1 ◽  
Dna Pooling ◽  
Single Marker Analysis ◽  
Backcross Inbred Lines ◽  
Dormancy Period ◽  
Single Marker ◽  
Putative Marker ◽  
Very High

Seed dormancy and shattering are important traits influencing the economics of rice farming. The genetic basis of dormancy and shattering traits were investigated in 174 Backcross Inbred Lines (BILs) derived from Oryza sativa cv. Swarna and O. nivara ac. CR100008. Significant variation was observed among the BILs for dormancy and shattering traits. Dormancy of 4-40 days was observed among BILs harvested at 35 days after heading and all the BILs attained > 80% germination by 6th week. Among all the BILs, least dormancy period (4 days) was found in SN-1, 13, 23, 25 and SN-28. Highest dormancy period (40 days) was found in 4 BILs i.e., SN-108, SN-116, SN-117 and SN-122 (40 days). None of the BILs were found to have non-shattering trait, while 2 BILs (SN-38 and SN-163) showed low shattering and 18 BILs were found with very high grain shattering percent. Of the 312 SSRs screened, 94 were polymorphic between the parents. A strategy of combining the DNA pooling from phenotypic extremes and genotyping was employed to detect the putative markers associated with dormancy and shattering traits. Single marker analysis revealed co-segregation of two putative markers RM488 on chromosome 1 and RM247 on chromosome 12 were with dormancy and shattering traits respectively. The putative marker RM488 identified is suitable for the marker-assisted transfer of the dormancy shown by O. nivara accession CR100008 for addressing pre harvest sprouting in modern cultivars. Interestingly, O. nivara type allele at RM247 was observed in BILs with low shattering phenotype.

scholarly journals Effect of Potassium Fertilization on Growth and Head Rice Yield of Rice (Oryza sativa L.) Merah Wangi Variety

2020 ◽  
Vol 21 (1) ◽  
pp. 61
Author(s):  
Wahyu Indra Duwi Fanata ◽  
Usmadi Usmadi ◽  
Galang Rizki Ramadhan
Keyword(s):  
Flowering Time ◽  
Plant Height ◽  
Oryza Sativa L ◽  
Block Design ◽  
Rice Variety ◽  
Quality Parameter ◽  
Control Treatment ◽  
Time Rate ◽  
Potassium Fertilization ◽  
Head Rice

The fertilization management is one strategy to optimize the quantity and quality of rice production. Our study was aimedto investigate the effect of potassium fertilization several agronomical parameters andthe rate of head rice after milling process. The study was conducted from March 2018 to July 2018 in the Agrotechnopark experimental field University of Jember in District of Sukorambi, Jember. Randomized Block Design (RBD) was used with the frequency of potassium chlorice (KCl) fertilization asthe single factor. The conducted potassium treatments were as follows: without KCl fertilization (control), one-time KCl fertilization, two times KCl fertilization, three times KCl fertilization and each fertilization level was conducted by sixrepetitions. The agronomic parameters such as plant height, tiller number, flowering time, rate of productive tillers, grain number per panicle, weight of 1000 grains, and percentage of filled grain were analyzed and percentage of head rice rate was determined as quality parameter. Our results showed that different treatment of potassium did not significantly affect the plant height, tillernumber, flowering time, rate of productive tillers, total grains per panicle, and weight of 1000 grains. On the other hands, the percentage of filled grainand the rate of head rice after milling were significantly increased by potassium fertilization. Compare to the control treatment, three times potassium fertilization resulted 9.24% and 13.83% increase of filled grain and head rice after two times milling, respectively. Our result suggest that potassium fertilization is important for grainquality of Merah Wangi rice variety. Keywords: Merah Wangi, potassium fertilization, growth, physical quality.

scholarly journals Bacteriophage Lambda Genomic Library Construction of Oryza sativa L. var. Pokkali

1970 ◽  
Vol 17 (2) ◽  
pp. 149-159
Author(s):  
Suhaila Rahman ◽  
Masa-aki Ohto ◽  
Zeba I. Seraj ◽  
Eduardo Blumwald
Keyword(s):  
Oryza Sativa ◽  
Salt Tolerance ◽  
Dna Isolation ◽  
Genomic Library ◽  
Oryza Sativa L ◽  
Rice Variety ◽  
Rice Chromosome ◽  
Chromosome 1 ◽  
Major Qtl ◽  
Key Words Rice

A major QTL called ‘Saltol’ on rice chromosome 1 has been identified as linked to salinity tolerance traits of the landrace, Pokkali. A genomic library of Pokkali rice variety was constructed in bacteriophage Lamda Fix II with 20 kb inserts. Plaques containing genes implicated to salt tolerance have been identified using probes homologous to S-Ad methionine synthetase (SAM) and cation chloride co-transporter after two rounds of hybridization. Clearly demarcated plaques of the two clones of interest have been lifted out for DNA isolation and subsequent characterization to be followed by subcloning into TAC vectors for Agrobacterium-mediated transformation of large genomic fragments into sensitive rice.  Key words: Rice, Pokkali, Genomic library, Lambda vector, Partial Fill-In, Titer  D.O.I. 10.3329/ptcb.v17i2.3235 Plant Tissue Cult. & Biotech. 17(2): 149-159, 2007 (December)

Marker-assisted identification of potential fertility restorers for the development of three-line hybrids in rice (Oryza sativa L.)

2020 ◽  
Vol 57 (3) ◽  
pp. 181-189
Author(s):  
Asma Majid ◽  
GA Parray ◽  
NR Sofi ◽  
Gazala H Khan ◽  
Showkat A Waza ◽  
...  
Keyword(s):  
Oryza Sativa L ◽  
Chromosome 1 ◽  
Limiting Factor ◽  
Kashmir Valley ◽  
Fertility Restorer ◽  
Yield And Quality ◽  
Restorer Genes ◽  
Increase Productivity ◽  
Fertility Restorers ◽  
Selection Of

Rice being a staple food crop of Kashmir valley, the focus is on enhancement of yield in order to meet the needs of ever-growing population.Identification of new parental lines is crucial for developing ecology-specific hybrids with ideal agronomic performance. Exploitation of heterosis in the form of hybrid rice technology can be one of the approaches to increase productivity in this crop, especially exploiting diversity among japonica lines can serve as an excellent route.A number of CMS lines suitable formountainous areas of Kashmir have been developed, however, the availability of promising restorer lines remains to be the major limitation for utilization of these lines.Identification of potential restorers acts as the main limiting factor for hybrid development in the Kashmir valley. Marker based screening for Rf3 and Rf4 fertility restorer genes can be helpful in rapid selection of restorer lines while dealing with the large quantity of genetic materials. In the present study, 100 rice germplasm were screened with the help of SSR markers, RM3148 and RM6100linked to Rf3 and Rf4 genes on chromosome 1 and 10, respectively. In total, 19 lines revealed the presence of both Rf3 and Rf4 genes. These lines amplified fertility restorer specific alleles for both the genes and may serve as potential restorers for obtaining heterotic rice hybrids. Further the germplasm lines were also evaluated for yield and quality traits.The present results would help in selection of suitable restorers along with preferred grain shape/size.

scholarly journals Integrative Transcriptomic and Proteomic Analysis Reveals an Alternative Molecular Network of Glutamine Synthetase 2 Corresponding to Nitrogen Deficiency in Rice (Oryza sativa L.)

2021 ◽  
Vol 22 (14) ◽  
pp. 7674
Author(s):  
Ting Liang ◽  
Zhengqing Yuan ◽  
Lu Fu ◽  
Menghan Zhu ◽  
Xiaoyun Luo ◽  
...  
Keyword(s):  
Glutamine Synthetase ◽  
Oryza Sativa L ◽  
Alternative Pathway ◽  
Rice Variety ◽  
Nitrogen Deficiency ◽  
Primary Root ◽  
Phenotypic Characterization ◽  
Glutathione Metabolism ◽  
N Assimilation ◽  
N Deficiency

Nitrogen (N) is an essential nutrient for plant growth and development. The root system architecture is a highly regulated morphological system, which is sensitive to the availability of nutrients, such as N. Phenotypic characterization of roots from LY9348 (a rice variety with high nitrogen use efficiency (NUE)) treated with 0.725 mM NH4NO3 (1/4N) was remarkable, especially primary root (PR) elongation, which was the highest. A comprehensive analysis was performed for transcriptome and proteome profiling of LY9348 roots between 1/4N and 2.9 mM NH4NO3 (1N) treatments. The results indicated 3908 differential expression genes (DEGs; 2569 upregulated and 1339 downregulated) and 411 differential abundance proteins (DAPs; 192 upregulated and 219 downregulated). Among all DAPs in the proteome, glutamine synthetase (GS2), a chloroplastic ammonium assimilation protein, was the most upregulated protein identified. The unexpected concentration of GS2 from the shoot to the root in the 1/4N treatment indicated that the presence of an alternative pathway of N assimilation regulated by GS2 in LY9348 corresponded to the low N signal, which was supported by GS enzyme activity and glutamine/glutamate (Gln/Glu) contents analysis. In addition, N transporters (NRT2.1, NRT2.2, NRT2.3, NRT2.4, NAR2.1, AMT1.3, AMT1.2, and putative AMT3.3) and N assimilators (NR2, GS1;1, GS1;2, GS1;3, NADH-GOGAT2, and AS2) were significantly induced during the long-term N-deficiency response at the transcription level (14 days). Moreover, the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis demonstrated that phenylpropanoid biosynthesis and glutathione metabolism were significantly modulated by N deficiency. Notably, many transcription factors and plant hormones were found to participate in root morphological adaptation. In conclusion, our study provides valuable information to further understand the response of rice roots to N-deficiency stress.

scholarly journals SAT0006 SIMULTANEOUS ANALYSIS OF ANTI-CCP, RHEUMATOID FACTOR, ANTI-PAD4 AND ANTI-CARBAMYLATED PROTEIN ANTIBODIES REVEALS INTERACTION EFFECTS WITH RESPONSE TO ANTI-TNF THERAPY IN RHEUMATOID ARTHRITIS

2020 ◽  
Vol 79 (Suppl 1) ◽  
pp. 933.2-934
Author(s):  
A. Julià ◽  
M. Lopez Lasanta ◽  
F. Blanco ◽  
A. Gómez ◽  
I. Haro ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Rheumatoid Factor ◽  
Clinical Response ◽  
Therapeutic Approach ◽  
Drug Response ◽  
Single Marker Analysis ◽  
Das28 Score ◽  
Positive Interaction ◽  
Single Marker ◽  
Necrosis Factor

Background:Blocking of the Tumor Necrosis Factor (TNF) activity is a successful therapeutic approach for 2 out of 3 Rheumatoid Arthritis patients. Identifying the patients that will not respond to this therapeutic approach is a major translational goal in RA. Association of seropositivity to rheumatoid factor (RF) or anti-cyclic-citrullinated antibodies (anti-CCP) with anti-TNF response has proven inconclusive, suggesting that other yet unexplored biomarkers could be more informative for this goal.Objectives:We tested the association of two recently introduced biomarkers in RA: anti-carbamylated protein antibodies (anti-CarP) and anti-peptidylarginine deiminase type 4 (anti-PAD4).Methods:A prospective cohort of n=80 RA patients starting anti-TNF therapy was recruited and levels for all four autoantibodies -RF, anti-CCP, anti-CarP and anti-PAD4- were measured at baseline. The change in DAS28 score between baseline and week 12 of therapy was used as the clinical endpoint.Results:Single marker-analysis showed no significant association with drug response. However, when testing for interactions between autoantibodies, we found highly significant associations with drug response. Anti-CCP and RF showed a positive interaction with the response to anti-TNF therapy (P=0.00068), and anti-PAD4 and antiCarP titers showed a negative interaction with the clinical response at week 12 (P=0.0062). Using an independent retrospective sample (n=199 patients), we validated the interaction between anti-CCP and RF with the clinical response to anti-TNF agents. (P=0.044).Conclusion:The results of this study show that interactions between antibodies are important in the response to anti-TNF therapy and suggest potential pathogenic relationships.Acknowledgments :We would like to thank the clinical researchers and patients participating in the IMID Consortium for their collaborationDisclosure of Interests:Antonio Julià: None declared, Maria Lopez Lasanta: None declared, Francisco Blanco: None declared, Antonio Gómez: None declared, Isabel Haro: None declared, Antonio Juan Mas: None declared, Alba Erra: None declared, Mª Luz García Vivar: None declared, Jordi Monfort: None declared, Simon Sánchez Fernandez: None declared, Isidoro González-Álvaro Grant/research support from: Roche Laboratories, Consultant of: Lilly, Sanofi, Paid instructor for: Lilly, Speakers bureau: Abbvie, MSD, Roche, Lilly, Mercedes Alperi-López: None declared, Raúl Castellanos: None declared, Antonio Fernandez-Nebro: None declared, Cesar Diaz Torne: None declared, Núria Palau: None declared, Raquel M Lastra: None declared, Jordi Lladós: None declared, Raimon Sanmarti: None declared, Sara Marsal: None declared

scholarly journals Performance of hybrid rice grown from separated tillers

2019 ◽  
Vol 17 (4) ◽  
pp. 507-513
Author(s):  
FN Kamarum Munira ◽  
Md. Parvez Anwar ◽  
Sabina Yeasmin ◽  
Md. Harun Or Rashid ◽  
Mst Farzana Rahman ◽  
...  
Keyword(s):  
Grain Yield ◽  
Plant Height ◽  
Hybrid Rice ◽  
Rice Variety ◽  
Field Laboratory ◽  
Split Plot ◽  
Rice Seeds ◽  
Four Levels ◽  
Plot Design

The experiment was conducted at the Agronomy Field Laboratory, Bangladesh Agricultural University, Mymensingh during July to November 2016 to investigate the performance of separated tillers of hybrid rice variety Dhani Gold. The experiment comprised three times of tiller transplantation viz. 3, 4 and 5 week aged tiller seedlings and four levels of number of tiller seedlings transplanted hill-1 viz. 2, 3, 4 and 5 tiller seedlings hill-1 laid out in a split plot design with 3 replications. The highest plant height was obtained when 3-week aged tiller seedlings were transplanted at the rate of 3 tiller seedlings hill-1. The highest number of total tillers hill-1 and number of effective tillers hill-1 were found when 3-week aged tiller seedlings were transplanted with 5 tiller seedlings hill-1. The highest grains panicle-1 was obtained when 4-week aged tiller seedlings were transplanted with 5 tiller seedlings hill-1. The highest grain yield was found when 3-week aged tiller seedlings were transplanted with 5 tiller seedlings hill-1. The lowest grain yield was found when 5-week aged tiller seedlings were transplanted with 2 tiller seedlings hill-1 in hybrid rice variety Dhani Gold. Based on the findings of the present study it may be concluded that 3-week old tiller seedlings of Dhani Gold should be transplanted at the rate of 4 or 5 tiller seedlings hill-1 for higher yield. Thus present study confirms the potentiality of growing hybrid rice from separated tillers in case of unavailability of seedlings or to minimize the seed cost of high value hybrid rice seeds. J Bangladesh Agril Univ 17(4): 507–513, 2019

scholarly journals Daya Gabung Beberapa Varietas Padi (Oryza sativa L.) pada Persilangan Dialel Menggunakan Metode Griffing II

2021 ◽  
pp. 84-93
Author(s):  
Anak Agung Ketut Sudharmawan ◽  
Baiq Erna Listiana ◽  
Sofi Rianti
Keyword(s):  
Oryza Sativa ◽  
Plant Height ◽  
Power Analysis ◽  
Oryza Sativa L ◽  
Panicle Length ◽  
Grain Number ◽  
Significant Difference ◽  
Diallel Crossing ◽  
High Yielding Varieties ◽  
Materials Used

Diallel crossing is a method that is widely used to determine the ability to combine each individual so that it can identify the potential elders to be used in crossbreeding programs to assemble high yielding varieties. This experiment aims to determine the combining power of dialelic crosses of several varieties of rice using the Griffing II method, carried out in the experimental field of the Faculty of Agriculture, Mataram University in Nyiur Lembang Village, Narmada District, West Lombok Regency, from February to July 2019. This experiment uses the method experiments carried out in the field. The materials used in this experiment were four parents, namely IPB 3S variety, Situ Patenggang variety, Inpari 32 and G11 strain and the phenotype of the cross between the four parents. The experimental results showed that the effects of general affinity and special affinity for all observed characters (plant height, number of productive tillers, number of non-productive tillers, panicle length, number of filled grain, number of empty grain and weight of 100 grains) were significantly different, except on the effect of general affinity for the number of non-productive tillers showed no significant difference. The results of the combined power analysis of the four elders were positive, so it can be said that the four elders would get good results when crossed with a number of other genotypes.

scholarly journals Effects of the components of conservation agriculture on the profitability of rice (Oryza sativa L.) in the Eastern Gangetic Plain of Bangladesh

2021 ◽  
Vol 7 (1) ◽  
pp. 333-337
Author(s):  
Hossain M ◽  
◽  
Begum M ◽  
Rahman M ◽  
Hashem A ◽  
...  
Keyword(s):  
Oryza Sativa L ◽  
Block Design ◽  
Rice Variety ◽  
Conservation Agriculture ◽  
Conventional Tillage ◽  
Cost Ratio ◽  
Gangetic Plain ◽  
Benefit Cost Ratio ◽  
On Farm ◽  
Residue Retention

A two year longer on-farm research on conservation agriculture was conducted at Bhangnamari area of Bangladesh during November-June in 2014-15 and 2015-16 to evaluate the performance of non-puddled rice cultivation under increased crop residue retention. The rice variety BRRI dhan28 was transplanted under puddled conventional tillage (CT) vs. non-puddled strip tillage (ST) with 50% standing residue (R50) vs. conventional no-residue (R0) practice. The treatments were arranged in a randomized complete block design with four replications. There were no significant yield differences between tillage practices and residue levels in 2014-15. But in the following year, ST yielded 9% more grain compared to CT leading to 22% higher BCR. Retention of 50% residue increased yield by 3% over no-residue, which contributed to 10% higher benefit-cost ratio (BCR). Results of this two year on-farm study confirmed that the ST combined with 50% residue retention yielded the highest grain yield (5.81 t ha-1) which contributed to produce the highest BCR (1.06).

scholarly journals Performance of Drought Tolerant Rice Varieties in Malkangiri District of South Eastern Ghat Zone of Odisha

2020 ◽  
pp. 157-162
Author(s):  
A. K. Rai ◽  
S. R. Dash ◽  
N Behera ◽  
T. K Behera ◽  
H Das
Keyword(s):  
Plant Height ◽  
Rice Variety ◽  
Economic Loss ◽  
Point Of View ◽  
Growth Stages ◽  
Rice Varieties ◽  
Economic Point ◽  
Eastern Ghat ◽  
South Eastern ◽  
Drought Tolerant

Drought is the major problem in India and occurrence at the time of critical growth stages, reduce crop yield significantly. The National Rice Research Institute, Cuttak, Odisha has developed so many drought tolerance rice varieties to reduce the economic loss due to drought. Krishi Vigyan Kendra, Malkangiri introduced NRRI released two rice varieties i.e. Sahbhagi Dhan and Satyabhama at framers' field to evaluate the performance of these drought tolerant varieties in South Eastern Ghat Zone of Odisha. The highest plant height was observed with the rice variety Satyabhama (104.1 cm). But, overall performance of Sahbhagi Dhan was better as compare to Satyabhama and local check verity (Khandagiri). Sahbhagi Dhan gave highest yield of 36.5 q/ha, which was statistically differed from others. Correlations study for Sahbhagi Dhan was assessed and observed the positive correlation for grain yield with all studied characters except plant height. For economic point of view, Sahbhagi Dhan recorded highest net return of Rs.19, 925/- per ha along with B:C ratio of 1.6 as compared to the rest two varieties.

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