scholarly journals Microarray testing in patients with systemic lupus erythematosus identifies a high prevalence of CpG DNA-binding antibodies

2021 ◽  
Vol 8 (1) ◽  
pp. e000531
Author(s):  
Tammo Brunekreef ◽  
Maarten Limper ◽  
Rowena Melchers ◽  
Linda Mathsson-Alm ◽  
Jorge Dias ◽  
...  
Keyword(s):  
Clinical Practice ◽  
Lupus Nephritis ◽  
Clinical Manifestations ◽  
Control Group ◽  
Control Groups ◽  
Blood Draw ◽  
Cpg Dna ◽  
Dna Motifs ◽  
Custom Made ◽  
Autoantibody Profiles

ObjectiveMany autoantibodies are known to be associated with SLE, although their role in clinical practice is limited because of low sensitivity and weak associations with clinical manifestations. There has been great interest in the discovery of new autoantibodies to use in clinical practice. In this study, we investigated 57 new and known antibodies and their potential for diagnostics or risk stratification.MethodsBetween 2014 and 2017, residual sera of all anti-dsDNA tests in the UMC Utrecht were stored in a biobank. This included sera of patients with SLE, patients with a diagnosis of another immune-mediated inflammatory disease (IMID), patients with low (non-IMID) or medium levels of clinical suspicion of SLE but no IMID diagnosis (Rest), and self-reported healthy blood bank donors. Diagnosis and (presence of) symptoms at each blood draw were retrospectively assessed in the patient records with the Utrecht Patient-Oriented Database using a newly developed text mining algorithm. Sera of patients were analysed for the presence of 57 autoantibodies with a custom-made immunofluorescent microarray. Signal intensity cut-offs for all antigens on the microarray were set to the 95th percentile of the non-IMID control group. Differences in prevalence of autoantibodies between patients with SLE and control groups were assessed.ResultsAutoantibody profiles of 483 patients with SLE were compared with autoantibody profiles of 1397 patients from 4 different control groups. Anti-dsDNA was the most distinguishing feature between patients with SLE and other patients, followed by antibodies against Cytosine-phosphate-Guanine (anti-CpG) DNA motifs (p<0.0001). Antibodies against CMV (cytomegalovirus) and ASCA (anti-Saccharomyces cerevisiae antibodies) were more prevalent in patients with SLE with (a history of) lupus nephritis than patients with SLE without nephritis.ConclusionAntibodies against CpG DNA motifs are prevalent in patients with SLE. Anti-CMV antibodies are associated with lupus nephritis.

scholarly journals Absence of Correlation between IL-28B Gene Polymorphisms and the Clinical Presentation of Chronic Hepatitis B in an Amazon Brazilian Population

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Simone Regina Souza da Silva Conde ◽  
Luciana L. Rocha ◽  
Vanessa M. Ferreira ◽  
Julius Caesar Mendes Soares Monteiro ◽  
Nathália Karla Fonseca Filgueiras ◽  
...  
Keyword(s):  
Chronic Hepatitis ◽  
Hepatitis B ◽  
Chronic Hepatitis B ◽  
Clinical Manifestations ◽  
Positive Control ◽  
Control Group ◽  
Similar Distribution ◽  
Control Groups ◽  
And Control

Objective. The present study investigated the prevalence of the IL-28B polymorphisms rs12979860 and rs8099917 in chronic hepatitis B patients from a case study in Eastern Amazonia.Methods. In total, 65 chronically infected HBV patients and 97 healthy subjects who were anti-HBc and anti-HBs positive (control group) were evaluated between May 2011 and December 2012. The groups of patients were designated as inactive carriers, chronic hepatitis without cirrhosis, and chronic hepatitis with cirrhosis based on clinical, pathological, biochemical, hematological, and virological variables. The patients were genotyped using quantitative real-time PCR.Results. The frequencies of the rs12979860 polymorphism were similar between the infected group (32.3% CC, 41.5% CT, and 26.2 TT) and the control population (35% CC, 47.4% CT, and 17.6% TT), and the frequencies of the rs8099917 polymorphism (7.7% GG, 35.4% GT, and 56.9% TT versus 7.2% GG, 35.1% GT, and 57.7% TT) were also similar in both groups. The associations between the rs12979860 and rs8099917 polymorphisms and the clinical manifestations were not statistically significant.Conclusion. In conclusion, these polymorphisms had a similar distribution between infected and control groups, indicating that they were not associated with susceptibility and the clinical evolution of hepatitis B in the examined population.

scholarly journals Clinical Manifestations and Changes of Haematological Markers among Active People Living in Polluted City: The Case of Douala, Cameroon

2021 ◽  
Vol 18 (2) ◽  
pp. 665
Author(s):  
Tiekwe Joseph Eloge ◽  
Ongbayokolak Nadine ◽  
Dabou Solange ◽  
Phélix Bruno Telefo ◽  
Isabella Annesi-Maesano
Keyword(s):  
Air Pollution ◽  
Air Pollutants ◽  
Urban Air Pollution ◽  
Clinical Manifestations ◽  
Biological Data ◽  
Sub Saharan Africa ◽  
Control Group ◽  
Urban Air ◽  
Control Groups ◽  
Systemic Symptoms

Urban air pollution, despite its dangerous health impact, is poorly studied in sub-Saharan Africa (sSA). Epidemiological data on this silent killer are almost non-existent for cities of Cameroon, which seems to be one of the sSA countries where populations are highly exposed to air pollutants. Objective: The present study was conducted in Douala city, and aimed at determining the association of urban air quality degradation with respiratory and systemic health in active populations exposed to air pollutants on a daily basis. Methods: A cross-sectional study was conducted from 2017 to 2019 in 1182 active people consisting of motorbikes drivers (MD), outdoor urban workers (UW), and fuel station sellers (FSS). A standardized questionnaire was used to document participants’ data. One hundred and twenty-six (126) motorbike drivers were selected to evaluate the relationship between haematological (white blood cells, platelets) and inflammatory (C-reactive protein—CRP) biomarkers, and air pollution; compared with those of a sixty-five (65) motorbike drivers’ control group enrolled in Dschang, another town situated at about 216.3 km from Douala. Results: Among those recruited in urban Douala, some respiratory disorders such as running nostrils, colds, common fever, sore throats, dry cough, wheezing, chest pain, shortness of breath and systemic symptoms such as headaches, eye irritation, conjunctivitis, watery eyes and general tiredness were very common among MD, UW, and FSS. Regarding biological data, blood monocytes, lymphocytes and CRP were found to be significantly increased among selected MD in Douala, compared to control groups in Dschang. Conversely, a more significant decrease in blood neutrophil level was observed among MD in Douala than control groups in Dschang. These changes of haematological markers were significantly associated with place of residence, site of activity, and daily duration. Conclusion: Our results suggest the risk of suffering from respiratory impairments and systemic symptoms with exposure to urban air pollution among active people working near highways in Douala.

scholarly journals Clinical manifestations of vegetative disregulation in video display unit users

2000 ◽  
Vol XXXII (3-4) ◽  
pp. 22-27
Author(s):  
L. M. Fatkhutdinova ◽  
M. F. Ismagilov ◽  
N. Kh. Amirov ◽  
L. R. Gafarova
Keyword(s):  
Clinical Manifestations ◽  
Control Group ◽  
Video Display ◽  
Control Groups ◽  
Migraine Pain ◽  
Display Unit ◽  
Individual Character ◽  
And Control ◽  
Video Display Unit

Prevalence of vegetative disfunction was studied in group of users of video display unit (VDU) (144 subjects) and in control group (71 subject). The main and control groups were similar in sex and age of subjects. Among video display unit users risk of vegetative disorders was 2,20 (range of the error 1,12 4,30). Hyperthermic and asthenic syndromes, migraine pain and vegetative crises were the most frequent manifestations of vegetative disorders. It was found that the risk of development of vegetative disorders in the group of video display unit users depended on sex, presence of dorsopathy and allergic states, individual character and natural vegetative tonus.

Biomechanical Effects of Kaempferol Treatments on the Bone Healing Process of Murine Tibia

2014 ◽  
Author(s):  
Hanjong Kim ◽  
Changwan Han ◽  
Otgonbayar Maidar ◽  
Sang-Soo Lee ◽  
Seonghun Park
Keyword(s):  
Mechanical Properties ◽  
Fracture Energy ◽  
Bone Healing ◽  
Healing Process ◽  
Treated Group ◽  
Fracture Load ◽  
Control Group ◽  
Control Groups ◽  
Custom Made ◽  
Bone Healing Process

Kaempferol is a typical flavonol-type flavonoid and has a protective effect on postmenopausal bone loss, and previous studies have reported that kaempferol treated groups show an increase in the callus size and bone mineral density as well as improvement in biomechanical behaviors in comparison with untreated control groups in the bone healing process. The present study aims at investigating the effect of kaempferol treatments on fractured murine tibia, by measuring kaempferol dose-dependent mechanical properties in the bone healing process of murine tibia fracture models. A stabilized fracture was generated at tibia by minor modification of the Hiltunen method for 8 weeks old ICR mice weighting 29.0 ∼ 30.5 g. Experimental mice were divided into 4 groups. Kaempferol of 0.2, 1.0, 5.0 mg/kg (body weight) with 20 % ethanol was administered to 3 groups and the remaining one group was only treated with 20 % ethanol as a control group. Three-point bending fracture tests were conducted to measure the mechanical properties (fracture load, fracture energy, stiffness) of murine tibiae at non-fractured regions near fracture sites 21 days after kaempferol treatments, via a custom-made biomechanical testing system (BTS, KST Co., Korea). The 5.0 mg/kg kaempferol treated group shows higher fracture load (20.54 ±5.04 N) than the control group (17.82 ±5.94 N). Fracture energy, total energy applied to tibia up to bone fracture, exhibited no significant differences between the control group and any of the kampferol treated groups, although both the 1.0 mg/kg kaempferol treated and control groups showed a little higher fracture energy than the 0.2 and 5.0 mg/kg kaempferol treated groups. Bone stiffness also did not show statistically significant differences between the control group and any of the kaempferol treated groups, with the highest stiffness value observed in the 1.0 mg/kg kaempferol treated group.

scholarly journals Clinical Practice of Cataract Special Care Standards in Nonophthalmic Wards

2022 ◽  
Vol 2022 ◽  
pp. 1-5
Author(s):  
Juan Yang ◽  
Lina Yang ◽  
Yawen Chen
Keyword(s):  
Clinical Practice ◽  
Health Knowledge ◽  
Clinical Manifestations ◽  
Corneal Edema ◽  
Control Group ◽  
Observation Group ◽  
Blurred Vision ◽  
Patient’S Satisfaction ◽  
Department Of Ophthalmology ◽  
Active Service

Cataract is a lens metabolism disease, which is caused by various factors, and leads to metamorphic lens proteins turbidity. Cataract commonly occurs in elderly patients, and majority of these patients have clinical manifestations of blurred vision and other symptoms. In this study, we explored the clinical practice and observations of cataract care criteria in nonophthalmic wards. To realize this, a total of one hundred and twenty (120) cataract patients, admitted to the East Department of Ophthalmology, Shanghai Sixth People’s Hospital, particularly from April 2019-2020, were divided into the control and observation groups, where 60 cases were added to each group. The control group received routine nursing, and observation group was treated with cataract care criteria based on the control group. The complication rate, health cognition, and patient’s satisfaction were compared with existing approaches. The incidence of corneal edema, anterior chamber hemorrhage, endophthalmitis, and incision infection, specifically in the observation group, was lower than that in the control group ( P < 0.05 ). Likewise, the number of hospitalization days and expenses, specifically in the observation group, were lower than those in the control group ( P < 0.05 ). Health knowledge and satisfaction scores of the observation group were higher than the control group ( P < 0.05 ). Active service, service attitude, psychological support, caring patients, and health education dissatisfaction rate of the observation group were lower than the control group ( P < 0.05 ). Finally, the standard of cataract care in nonophthalmic wards reduces the incidence of complications, improve health awareness of patients, and help to improve satisfaction of patients with nurses. Furthermore, it is worthy of promotion and application particularly in traditional hospitals.

Conjugated Linoleic Acid Causes a Marked Increase in Liver α-Tocopherol and Liver α-Tocopherol Transfer Protein in C57BL/6 J Mice

2010 ◽  
Vol 80 (1) ◽  
pp. 65-73 ◽  
Author(s):  
Pei-Min Chao ◽  
Wan-Hsuan Chen ◽  
Chun-Huei Liao ◽  
Huey-Mei Shaw
Keyword(s):  
Antioxidant Enzymes ◽  
Linoleic Acid ◽  
Conjugated Linoleic Acid ◽  
Thiobarbituric Acid ◽  
Control Group ◽  
Thiobarbituric Acid Reactive Substances ◽  
Control Groups ◽  
Protein Levels ◽  
Transfer Protein ◽  
And Control

Conjugated linoleic acid (CLA) is a collective term for the positional and geometric isomers of a conjugated diene of linoleic acid (C18:2, n-6). The aims of the present study were to evaluate whether levels of hepatic α-tocopherol, α-tocopherol transfer protein (α-TTP), and antioxidant enzymes in mice were affected by a CLA-supplemented diet. C57BL/6 J mice were divided into the CLA and control groups, which were fed, respectively, a 5 % fat diet with or without 1 g/100 g of CLA (1:1 mixture of cis-9, trans-11 and trans-10, cis-12) for four weeks. α-Tocopherol levels in plasma and liver were significantly higher in the CLA group than in the control group. Liver α-TTP levels were also significantly increased in the CLA group, the α-TTP/β-actin ratio being 2.5-fold higher than that in control mice (p<0.01). Thiobarbituric acid-reactive substances were significantly decreased in the CLA group (p<0.01). There were no significant differences between the two groups in levels of three antioxidant enzymes (superoxide dismutase, glutathione peroxidase, and catalase). The accumulation of liver α-tocopherol seen with the CLA diet can be attributed to the antioxidant potential of CLA and the ability of α-TTP induction. The lack of changes in antioxidant enzyme protein levels and the reduced lipid peroxidation in the liver of CLA mice are due to α-tocopherol accumulation.

Sri Lankan Cleft Lip and Palate Study Model Analysis: Clefts of the Secondary Palate

1993 ◽  
Vol 30 (2) ◽  
pp. 227-230 ◽  
Author(s):  
Andrew Mccance ◽  
David Roberts-Harry ◽  
Martyn Sherriff ◽  
Michael Mars ◽  
William J.B. Houston
Keyword(s):  
Cleft Lip ◽  
Cleft Lip And Palate ◽  
Model Analysis ◽  
Control Group ◽  
Tooth Size ◽  
Sri Lankan ◽  
Control Groups ◽  
Secondary Palate ◽  
Comparable Control ◽  
And Control

The study models of a group of adult Sri Lankan patients with clefts of the secondary palate were investigated. Tooth-size and arch-dimension comparisons were made with a comparable control group. Significant differences were found between the cleft and control groups in tooth sizes, chord lengths, and arch widths. The cleft group dimensions were generally smaller than those of the control group. Overjets were larger in the cleft group.

THE EFFECT OF APPLYING ANIMAL CARTOON PICTURES ON STUDENTS’ ACHIEVEMENT IN WRITING NARRATIVE TEXT

2017 ◽  
Vol 6 (1) ◽  
Author(s):  
Hasanul Arifin Zul And Masitowarni Siregar
Keyword(s):  
T Test ◽  
Narrative Text ◽  
Control Group ◽  
First Year ◽  
Writing Achievement ◽  
Control Groups ◽  
Post Test ◽  
Written Test ◽  
And Control ◽  
Experimental Group

This thesis is focused on the investigation of the effect of applying animal cartoon pictures on students’ achievement in writing narrative text. This study aims to find whether applying animal cartoon pictures significantly affect the students’ writing achievement or not. The data in this study were obtained by administering a written test. The population was the 2015/2016 first year (grade XI) of SMA Swasta Nusantara Lubuk Pakam and 66 students were taken as the sample by using random sampling. The sample was divided into two groups, experimental and control groups. The experimental group was taught by applying animal cartoon pictures while the control group without animal cartoon pictures (x = lecturing). The data were taken the scores from the pre-test and post-test to both experimental and control groups. These data were analyzed by using t-test. The result of computing the t-test obviously showed that t-observed is higher than t-table (5.21 >1,67) with the degree of freedom 64 (df =N-2) at the level significance 0,05 one tail test. It showed that the application of animal cartoon pictures significantly affected the students of SMA Swasta Nusantara Lubuk Pakam achievement in writing narrative text.

THE EFFECT OF APPLYING DEMONSTRATION METHOD ON STUDENTS’ ACHIEVEMENT IN SPEAKING SKILL AT THE THIRD GRADE OF SMK PAB 3 MEDAN ESTATE

2015 ◽  
Vol 4 (2) ◽  
Author(s):  
Meryanti Napitupulu And Anni Holila Pulungan
Keyword(s):  
High School ◽  
Control Group ◽  
Vocational High School ◽  
Control Groups ◽  
Speaking Test ◽  
The Third ◽  
Speaking Skill ◽  
The Subject ◽  
And Control ◽  
Experimental Group

This study was conducted as an attempt to discover the effect of applying Demonstration Method on students’ achievement in speaking skill. It was an experimental research. The subject was students of Grade XII, Vocational High School (Sekolah Menengah Kejuruan: SMK), which consisted of 79 students. The research was divided into two groups: experimental and control groups. The instrument used to collect the data was speaking test. To obtain the reliability of the test, the writer applied Kuder Richardson 21 formula. The result of the reliability was 0.7, and it was found that the test was reliable. The data were analyzed by using t-test formula. The analysis showed that the scores of the students in the experimental group were significantly higher than the scores of the students in the control group at the level of significant m = 0.05 with the degree of freedom (df) 77, t-observed value 8.9 > t-table value 1.99. The findings indicate that using Demonstration Method significantly affected the students’ achievement in speaking skill. So, English teachers are suggested to use Demonstration Method in order to improve students’ achievement in speaking skill.

Effects of Virgin Coconut Oil as Adjunct Therapy in the Treatment of Allergic Rhinitis

2016 ◽  
Vol 1 (1) ◽  
pp. 22
Author(s):  
Nazli Zainuddin ◽  
Nurul Azira Mohd Shah ◽  
Rosdan Salim
Keyword(s):  
Allergic Rhinitis ◽  
Side Effects ◽  
Coconut Oil ◽  
Test Group ◽  
Nasal Symptom ◽  
Control Group ◽  
Virgin Coconut Oil ◽  
Control Groups ◽  
Significant Difference ◽  
And Control

Introduction: The role of virgin coconut oil in the treatment of allergic rhinitis is controversial. Thus, the aim of the present study is to determine the effects of virgin coconut oil ingestion, in addition to standard medications, on allergic rhinitis. We also studied the side effects of consumption of virgin coconut oil. Methods: Fifty two subjects were equally divided into test and control groups. All subjects received a daily dose of 10mg of loratadine for 28 days. The test group was given 10ml of virgin coconut oil three times a day in addition to loratadine. The symptoms of allergic rhinitis were scored at the beginning and end of the study. Results:, the symptom score were divided into nasal and non-nasal symptom scores. Sneezing score showed a significant difference, however the score was more in control group than test group, indicating that improvement in symptom was more in control group. The rest of the nasal symptom and non-nasal symptom score showed no significant difference between test and control groups. Approximately 58% of the test subjects developed side effects from consumption of virgin coconut oil, mainly gastrointestinal side effects. Conclusion: In the present study, ingestion of virgin coconut oil does not improve the overall and individual symptoms of allergic rhinitis, furthermore it has side effects.

Sign in / Sign up

Export Citation Format

Share Document