scholarly journals Sedative and Hypnotic Activities of the Methanolic and Aqueous Extracts ofLavandula officinalisfrom Morocco

2012 ◽  
Vol 2012 ◽  
pp. 1-5 ◽  
Author(s):  
Rachad Alnamer ◽  
Katim Alaoui ◽  
El Houcine Bouidida ◽  
Abdelaziz Benjouad ◽  
Yahia Cherrah
Keyword(s):  
Aqueous Extract ◽  
Methanolic Extract ◽  
Folk Medicine ◽  
Oral Route ◽  
Sedative Effect ◽  
Aqueous Extracts ◽  
Hypnotic Effect ◽  
Wide Range ◽  
Hole Board ◽  
Lavandula Officinalis

We evaluate the sedative and hypnotic activities of the methanolic and aqueous extract ofLavandula officinalisL. on central nervous system (CNS). In this study, the effect of the methanolic and aqueous extracts of this plant was investigated in a battery of behavioural models in mice. Stems and flowers ofLavandula officinalisL. have several therapeutic applications in folk medicine in curing or managing a wide range of diseases, including insomnia. The methanolic extract produced significant sedative effect at the doses of 200, 400, and 600 mg/kg (by oral route), compared to reference substance diazepam (DZP), and an hypnotic effect at the doses of 800 and 1000 mg/kg while the treatment of mice with the aqueous extract at the doses of 200 and 400 mg/kg via oral pathway significantly reduced in both the reestablishment time and number of head dips during the traction and hole-board tests. In conclusion, these results suggest that the methanolic and aqueous extracts ofLavandula officinalispossess potent sedative and hypnotic activities, which supported its therapeutic use for insomnia.

The Role of Temperature and Heterotrophism in Determining Crude Oil Toxicity

1975 ◽  
Vol 10 (1) ◽  
pp. 73-83
Author(s):  
J.E.S. Graham ◽  
T.C. Hutchinson
Keyword(s):  
Crude Oil ◽  
Aqueous Extract ◽  
Chlorella Vulgaris ◽  
Nutrient Medium ◽  
General Pattern ◽  
Growth Stimulation ◽  
Aqueous Extracts ◽  
Chlamydomonas Eugametos ◽  
Wide Range ◽  
Oil Toxicity

Abstract Crude oil spills are increasingly likely to occur from drilling, pumping and transportation activities as oil development proceeds at a rapid pace. These spills may occur over the wide range of climatic conditions which obtain in Canada. Little is known of oil toxicity at different temperatures; consequently, laboratory studies were made of the variability of the toxicity of aqueous extracts of a Norman Wells crude oil to freshwater algae over the temperature range 5°C to 35°C. Two unicellular green algae were studied: Chlamydomonas eugametos and Chlorella vulgaris. Their response (measured by cell numbers) varied with temperature and species. Whereas Chlamydomonas eugametos showed a general pattern of growth inhibition by oil at all temperatures with maximum inhibition at 25°C, Chlorella vulgaris showed general growth stimulation by oil with maximum stimulation at 25°C, this temperature was chosen for all further experimentation. All experiments were done using unialgal cultures and sterile technique. Cells were grown in 50 ml of nutrient medium (BBM) in 125 ml Erlenmeyer flasks. Such flasks allow gas exchange and permit loss of volatile hydrocarbons. Aqueous extracts were made by slowly stirring 5% crude oil with the nutrient medium for six hours using a magnetic mixer. The extract was then allowed to sit for two to four hours before the lower fraction was drawn off for use. Experiments were carried out in controlled environment chambers (±2°C) with a twelve hour light-dark cycle. All further experiments used a similar methodology. (Note: Chlamydomonas eugametos experiments were carried out on a rotary shaker at 125 rpm.) An attempt was made to determine the reason for the remarkable stimulation in growth of Chlorella vulgaris #29 at 25°C. This organism has been described in the literature as heterotrophic. Thus three reasons for stimulation seemed possible: 1. heterotrophic uptake of hydrocarbons directly from solution; 2. heterotrophic uptake of organic compounds formed or released by microbial breakdown of hydrocarbons (the aqueous extract of crude was not sterile); or 3. the use of CO2 released to solution by microbial respiration. The original experiment was repeated in the dark at 20°C to determine if stimulation still occurred. It did not, since cells exposed to the aqueous extract decreased in numbers. However, after two weeks the cells were illuminated and even though experimental flasks started off with depleted populations, they outgrew the control cells within two weeks. This suggested that if stimulation was related to heterotrophism, it must, at least in this case, have been the unusual case of photoheterotrophism. The reasons for this stimulation of growth are currently under investigation. Several methods are being employed to investigate the suspected heterotrophism. Experiments will be done to determine whether light energy is essential to the stimulation. Two varieties of Chlorella vulgaris, i.e. #29 and #260 are heterotrophic and autotrophic respectively, are to be used in experiments. Sterile aqueous extracts made by pressure ultrafiltration will be used. These experiments should determine whether algal growth stimulation is related to heterotrophism or whether microbial degradation of hydrocarbons is the real source of stimulation. Although the toxicity of crude oil may be rapidly ameliorated by physical and/or biological phenomena, one must still be aware of the possibility of a large input of organic carbon causing extensive eutrophication. Thus both toxicity and eutrophication will cause a selection, in terms of survival, in a natural environment. It is evident that although an oil spill may not totally destroy an ecosystem, it will certainly alter its natural composition considerably.

scholarly journals Inhibitory Effect of the Methanolic Extract of Verbascum latisepalum Hub.-Mor. on Endothelium-Dependent Relaxation in Rat Thoracic Aorta

2014 ◽  
Vol 69 (5-6) ◽  
pp. 219-225
Author(s):  
T. Emrah Bozkurt ◽  
I. Irem Tatli ◽  
Cigdem Kahraman ◽  
Zeliha S. Akdemir ◽  
Inci Sahin-Erdemli
Keyword(s):  
Methanolic Extract ◽  
Folk Medicine ◽  
Inhibitory Effect ◽  
Relaxation Response ◽  
Spectral Techniques ◽  
Wide Range ◽  
Bioassay Guided Fractionation ◽  
Rat Thoracic Aorta ◽  
Skin Conditions ◽  
Endothelium Dependent Relaxation

The leaves and flowers of Verbascum species are used to treat respiratory disorders, haemorrhoids, rheumatic pain, and wounds as well as for the treatment of eczema and other types of inflammatory skin conditions in traditional Turkish medicine. We examined the effect of the methanolic extract of the aerial parts of Verbascum latisepalum Hub.-Mor. on the endothelium-dependent relaxation response in rat aortic rings which is mediated by nitric oxide (NO). Six fractions, A- F, were obtained from the methanolic extract through bioassay-guided fractionation procedures. The phenylethanoid glycoside verbascoside was isolated from fraction D and its structure elucidated by spectral techniques. The inhibitory effects of the extract, its fractions, and verbascoside on the acetylcholineinduced relaxation response in phenylephrine-precontracted aorta was examined in the absence and presence of L-arginine, a precursor in the synthesis of NO. The observation that the effects of the methanolic extract, of fraction D, and of verbascoside were reversed by L-arginine, indicates that verbascoside has an inhibitory effect on the synthesis of NO. This effect should be taken into consideration in view of the wide range of uses of Verbascum species in Turkish folk medicine

scholarly journals Preliminary Study on Antifungal Mechanism of Aqueous Extract of Cnidium monnieri Against Trichophyton rubrum

2021 ◽  
Vol 12 ◽  
Author(s):  
Cao Yanyun ◽  
Tang Ying ◽  
Kong Wei ◽  
Fang Hua ◽  
Zhu Haijun ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Aqueous Extract ◽  
Trichophyton Rubrum ◽  
Economic Value ◽  
Differentially Expressed ◽  
Control Group ◽  
Potential Candidate ◽  
Aqueous Extracts ◽  
Antifungal Mechanism ◽  
Wide Range

Trichoderma rubrum (T. rubrum) is one of the important pathogens because it is the cause of most dermatomycosis. The treatment of Trichophyton rubrum infection is time-consuming and very expensive; it is easy for the infections to reoccur, leading to therapeutic failures, persistence, and chronic infection. These issues have inspired researchers to study natural alternative therapies instead. Cnidium monnieri (L.), as a kind of traditional Chinese medicine, has a variety of pharmacological activities and a wide range of applications, so it has a high potential for researching and economic value. We detected the effect of aqueous extract of C. monnieri (L.) on the activity of T. rubrum by Cell Count Kit-8 assay (CCK-8), and we found that 128 and 256 μg/ml of aqueous extracts of C. monnieri (L.) co-cultured with T. rubrum for 24 h showed the inhibitory effect on T. rubrum. The results of scanning electron microscopy (SEM) and transmission electron microscopy (TEM) confirmed that aqueous extract of C. monnieri (L.) damaged the T. rubrum. At the same time, mass spectrometry screening with T. rubrum before and after the treatment of 256 μg/ml of aqueous extracts of C. monnieri (L.) showed that 966 differentially expressed proteins were detected, including 524 upregulated differentially expressed genes (DEGs) and 442 downregulated DEGs. The most significantly downregulated protein was chitin synthase (CHS); and the results of qRT-PCR and Western blotting demonstrated that the expression level of CHS was downregulated in the 256 μg/ml group compared with the control group. The study showed that the aqueous extract of C. monnieri (L.) could destroy the morphology of mycelia and the internal structure of T. rubrum, and it could inhibit the growth of T. rubrum. The antifungal effect of aqueous extract of C. monnieri (L.) may be related to the downregulation of the expression of CHS in T. rubrum, and CHS may be one of the potential targets of its antifungal mechanism. We concluded that aqueous extract from C. monnieri (L.) may be a potential candidate for antifungal agents.

scholarly journals ANTI-DIABETIC ACTIVITY

2018 ◽  
Vol 25 (04) ◽  
pp. 502-508
Author(s):  
Muhammad Saleh Faisal ◽  
Usman Nawaz ◽  
Abdul Matin Khan
Keyword(s):  
Body Weight ◽  
Blood Level ◽  
Aqueous Extract ◽  
Study Design ◽  
Methanolic Extract ◽  
P Value ◽  
Aqueous Extracts ◽  
Methanolic Extracts ◽  
Diabetic Rabbits

Objectives: The study was designed to evaluate the hypoglycaemic effects ofCassia sophera’s powdered seeds and its different fractions. Study Design: An experimentalstudy. Setting: Faculty of Veterinary, University of Agriculture, Faisalabad, Pakistan. Period:November 2014 to February 2017. Methodology: Initial experiments were designed to find outthe hypoglycaemic effects of Cassia sophera powdered seeds in increasing doses i.e. 2, 3, 4g/kg body weight at 0, 2, 4, 8, 12 and 24 hours intervals. The effects of methanolic and aqueousextracts equivalent to 4g/kg body weight of Cassia sophera seeds were also studied in bothnormal and diabetic rabbits. Results: At 4 hours interval, 2grams/kg body weight of grindedseeds results no significant decrease in mean blood level of glucose. At 8 and 12 hours intervals,a significant decrease while at 4, 8 and 12 hours a highly significant reduction were observedwith both 3g/kg and 4g/kg body weight. With methanolic and aqueous extracts equivalent to4g/kg body weight, normal rabbits showed significant decrease i.e. with P value less than 0.05at 8 and 12 hours. In addition, the methanolic extract of Cassia sophera seeds produced betterhypoglycaemia as compared to aqueous extract. The effects of these extracts were comparableto Acetohexamide. The same study was also done in alloxan-induced diabetic rabbits and it wasnoticed that Cassia sophera powdered seeds caused significant decrease in blood glucoselevel at increasing doses 2, 3 and 4gram/kg of body weight and highly significant reduction(with P value less than 0.001) in blood level of glucose was noticed with methanolic extracts at4 hours interval which continued upto 8 hours while treatment with aqueous extract with Cassiasophera seeds showed highly significant decrease at 12 hours. Conclusion: It is concludedthat powdered seeds of Cassia sophera constitutes more than one type of hypoglycaemicelements, which have exerted a significant and consistent hypoglycaemic effects in normal andalloxan treated diabetic rabbits.

scholarly journals Phytochemical Profile and Biological Activities of the Extracts from Two Oenanthe Species (O. aquatica and O. silaifolia)

2021 ◽  
Vol 15 (1) ◽  
pp. 50
Author(s):  
Łukasz Świątek ◽  
Elwira Sieniawska ◽  
Mohamad Fawzi Mahomoodally ◽  
Nabeelah Bibi Sadeer ◽  
Krzysztof Kamil Wojtanowski ◽  
...  
Keyword(s):  
Aqueous Extract ◽  
Methanolic Extract ◽  
Biological Activities ◽  
Radical Scavenging ◽  
Scientific Data ◽  
Aqueous Extracts ◽  
Reducing Ability ◽  
Trolox Equivalent ◽  
Phytochemical Profile ◽  
Hsv 1

This study presents the evaluation of biological activities and chemical profiling of Oenanthe aquatica (L.) Poir. and Oenanthe silaifolia M. Bieb. The phytochemical profile, antioxidant, enzyme inhibitory, cytotoxic and antiviral activities of the methanolic and aqueous extracts were investigated. The aqueous extract of O. aquatica possessing the highest content of phenolics (60.85 mg gallic acid equivalent/g extract), also exhibited the strongest radical scavenging potential against 2,2-diphenyl-1-picrylhydrazyl and 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (79.46 and 148.66 mg Trolox equivalent/g extract, respectively), the highest reducing ability (207.59 and 107.27 mg Trolox equivalent/g extract, for cupric reducing antioxidant capacity and ferric reducing antioxidant activity, respectively), metal chelating potential (33.91 mg ethylenediaminetetraacetic acid equivalent/g extract) and total antioxidant ability (1.60 mmol Trolox equivalent/g extract). Liquid chromatography-electrospray ionization-quadrupole time-of-flight-mass spectrometry (LC-ESI-QTOF-MS/MS) permitted tentative identification of compounds from simple organic acids, phenolic acids, coumarins, flavonoids and their glycosides in O. aquatica and O. silaifolia extracts. The methanolic extract of O. aquatica substantially depressed acetylcholinesterase (3.67 mg galantamine equivalent/g extract), tyrosinase (126.66 mg kojic acid equivalent/g extract), and α-amylase (0.83 mmol acarbose equivalent/g extract) enzymes. The methanolic extract of O. silaifolia showed highest enzymatic inhibitory property against butyrylcholinesterase, and its aqueous extract depressed α-glucosidase activity (0.26 mmol acarbose equivalent/g extract). All tested extracts exerted selective toxicity towards cancer cell lines, and the highest anticancer potential was found for O. aquatica aqueous extract on FaDu and HeLa cells with CC50 of 57.36 and 47.16 µg/mL, respectively. Significant antiviral activity against HSV-1 (HHV-1) was found for both aqueous extracts in concentrations of 1000 µg/mL, which inhibited the HSV-1 cytopathic effect (CPE) in virus infected VERO cells and reduced the virus infective titer by more than 3 log (logCCID50/mL). This study has produced critical scientific data on O. aquatica and O. silaifolia, which are potential contenders for the development of novel phyto-pharmaceuticals.

scholarly journals Effects of Ultra-Highly Diluted Solutions of Palicourea marcgravii St. Hill in Rats Poisoned by Aqueous Extracts of This Plant

2021 ◽  
Vol 7 (25) ◽  
pp. 193-198
Author(s):  
Luiz Figueira Pinto ◽  
Lílian Rangel Castilhos ◽  
João Telhado ◽  
Ticiana Nascimento França ◽  
Marilene Farias Brito ◽  
...  
Keyword(s):  
Aqueous Extract ◽  
Wistar Rats ◽  
Clinical Signs ◽  
Economic Loss ◽  
Oral Route ◽  
Aqueous Extracts ◽  
Palabras Clave ◽  
High Dilutions ◽  
The Times ◽  
Nervous Excitability

Palicourea marcgravii , a shrub causing sudden death in cattle, is a major cause of economic loss to breeders in Brazil. The aim of the present study was to evaluate the effects of high diluted solutions 6cH and 30cH of P. marcgravii, on the development of tolerance to the toxic effects of this plant. 14 adult Wistar rats were divided into 3 test groups. AE group was composed of 4 rats receiving aqueous extract of P. marcgravii intragastrically at a dose of 2g/kg. Groups HD6AE and HD30AE comprised 5 rats each. Animals in these groups received 1 mL of 6cH and 30cH solutions of P. marcgravii respectively by oral route 3 times a day, for 8 days. At the end of this period, they were intragastrically intoxicated with 2g/kg of aqueous extract of P. marcgravii, receiving the corresponding high diluted preparation hourly until death. Main symptoms were nervous excitability and convulsions. Even though the times for onset of the first clinical signs, convulsions and death was slightly longer in the animals in group HD30AE when compared to group AE, no evidence indicating that the highly diluted preparations increase tolerance to intoxication by P. marcgravii was found. Keywords: Palicourea marcgravii; Intoxication; Isopathy; High dilutions; Wistar rats.   Efeitos de altas diluições de Palicourea marcgravii St Hill em ratos intoxicados com extrato aquoso dessa planta. Resumo Palicourea marcgravii, arbusto que causa morte súbita no gado, é um dos principais motivos de perda para os criadores brasileiros. O objetivo do presente estudo foi avaliar o efeito de altas diluições de P. marcgravii 6cH e 30cH no desenvolvimento de tolerância aos efeitos tóxicos dessa planta. 14 ratos Wistar adultos foram divididos em 3 grupos experimentais: AE (extrato aquoso), composto de 4 ratos que receberam extrato aquoso de P. marcgravii por via intragástrica na dose de 2g/kg; e HD6AE e HD30AE, compostos de 5 animais cada um, que receberam 1 ml, respectivamente, das soluções 6cH e 30cH de P. marcgravii via oral, 3 vezes ao dia por 8 dias. No final esse período, foram intoxicados com 2g/kg de extrato aquoso de P. marcgravii por via intragástrica, continuando a receber a correspondente alta diluição de forma horária até a morte. Os principais sintomas observados foram excitabilidade nervosa e convulsões. Embora o início para o começo dos primeiros sinais clínicos, convulsões e morte foi ligeiramente mais longo no grupo HD30AE por comparação com o grupo AE, não foram achadas evidéncias de que altas diluições aumentem a tolerância na intoxicação por P. marcgravii. Palavras-chave: Palicourea marcgravii; Intoxicação; Isopatia; Altas diluições; Ratos Wistar.   Efectos de altas diluciones de Palicourea marcgravii St Hill en ratones intoxicados con extracto acuoso de esta planta Resumen Palicourea marcgravii, arbusto que causa muerte súbita en ganado, es uno de los principales motivos de pérdidas en ganadería. El objetivo de este trabajo fue evaluar el efecto de altas diluciones de P. marcgravii 6cH y 30cH en el desarrollo de tolerancia a los efectos tóxicos de esta planta. 14 ratones Wistar adultos fueron divididos e 3 grupos experimentales: AE (extracto acuoso), compuesto por 4 ratones, que recibieron extracto acuoso de P. marcgravii por vía intragástrica, en dosis de 2g/kg; y HD6AE y HD30AE, compuestos por 5 animales cada uno, que recibieron respectivamente 1 ml de las soluciones 6cH y 30cH de P. marcgravii vía oral, 3 veces al día, durante 8 días. Al final de este plazo, los animales fueron intoxicados con 2g/kg de extracto acuoso de P. marcgravii por vía intragástrica, mientras continuaron recibiendo la correspondiente alta dilución cada hora, hasta el deceso. Los principales síntomas observados fueron excitabilidad nerviosa y convulsiones. Aunque el tiempo hasta el comienzo de los primeros signos clínicos, convulsiones y muerte fue mayor en el grupo HD30AE en comparación al grupo AE, no fueron encontradas evidencias de que las altas diluciones aumentasen la tolerancia a la intoxicación por P. marcgravii. Palabras-clave: Palicourea marcgravii; Intoxicación; Isopatia; Altas diluçiones; Ratones Wistar.   Correspondence author: Luiz Figueira Pinto, [email protected] How to cite this article: Pinto LF, Castilhos LR, Telhado J, França TN, Brito MF, Peixoto PV. Effects of High Diluted Solutions of Palicourea marcgravii St Hill in Rats Poisoned by Aqueous Extracts of This Plant. Int J High Dilution Res [online]. 2008 [cited YYYY Mmm DD]; 7(25): 193-198. Available from: http://journal.giri-society.org/index.php/ijhdr/article/view/301/372.  

scholarly journals Phytochemical screening and antioxidant evaluation of millet varieties of Pakistan

2020 ◽  
Vol 12 (1) ◽  
pp. 118-122
Author(s):  
Nosheen Naz ◽  
Allah Rukha ◽  
Nighat Raza ◽  
Umar Farooq ◽  
Kashif Razzaq ◽  
...  
Keyword(s):  
Aqueous Extract ◽  
Methanolic Extract ◽  
Aqueous Extracts ◽  
Phytochemical Screening ◽  
Mineral Analysis ◽  
Oxidative Potential ◽  
Methanolic Extracts ◽  
Antioxidant Evaluation ◽  
Average Quantity ◽  
Β Carotene

The current research was planned to measure the comparative phytochemical and anti-oxidative potential of aqueous and methanolic extracts of two indigenously grown millet varieties of Pakistan. The locally available millet varieties, i.e. MB-87 and AF-POP flours were chemically characterized through the proximate and mineral analysis. The antioxidant extract was prepared in water and methanol. The extracts were screened for saponins, terpenoids, flavonoids and tannins; methanolic extract of MB-87 and AF-POP showed flavonoids in an average quantity (++), whilst aqueous extract had only trace (+) values. The phytochemical screening showed the presence of saponins only in the aqueous extract of both varieties. However, terpenoids and tannins were present in both methanolic and aqueous extracts. During antioxidant evaluation, millet methanolic extracts showed higher level of TPC and DPPH of MB-87 and AF-POP as 0.30±0.001 & 0.23±0.0012 mg GAE/g and 48±0.96 &46±1.09%, respectively. However, the β-carotene activity of the aqueous extract of both varieties (MB-87 and AF-POP) was recorded higher. In a nutshell, the methanolic extract of MB-87 has an appreciable atioxidant profile. Furher research should be planned to screen the important constituents of Pakistani millet varieties.

Antioxidant and Anti-inflammatory Activities of Organic and Aqueous Extracts of Northeast Algerian Marrubium vulgare

2018 ◽  
Vol 16 (S1) ◽  
pp. S119-S129
Author(s):  
I. Namoune ◽  
B. Khettal ◽  
A.M. Assaf ◽  
S. Elhayek ◽  
L. Arrar
Keyword(s):  
Ethyl Acetate ◽  
Methanolic Extract ◽  
Ethyl Acetate Extract ◽  
Interleukin 1 ◽  
Total Phenolic ◽  
Dependent Manner ◽  
Aqueous Extracts ◽  
Traditional Use ◽  
Marrubium Vulgare ◽  
Anti Inflammatory

Marrubium vulgare (Lamiaceae) is frequently used in traditional medicine to treat many illnesses from ancient times. Its beneficial effects include antibacterial, antioedematogenic, and analgesic activities. This study was designed to evaluate the antioxidant and anti-inflammatory activities of organic and aqueous extracts of the leaves, the flowers, the stems, and the roots of Marrubium vulgare. The total phenolic and flavonoid contents as well as the antioxidant and the anti-inflammatory effects of methanol, chloroform, ethyl acetate, and aqueous extracts have been investigated by using different in-vitro methods. It was found that the ethyl acetate extract from Marrubium vulgare stems had the highest total phenolic content, while the ethyl acetate extract from the leaves yielded a high concentration of flavonoids. The ethyl acetate extract from the stems exhibited the highest activity in scavenging of 2,2-diphenyl- 1-picrylhydrazyl (DPPH), as well as in protecting erythrocytes. The leaves aqueous extract exhibited the highest ferrous chelating activity and its methanolic extract was found to be the strongest inhibitor of lipid peroxidation in β-carotene bleaching assay. The leaves chloroform extracts as well as the flowers methanol, chloroform, and ethyl acetate extracts were found to decrease the pro-inflammatory tumor necrosis factor alpha (TNF-α) cytokine levels in a dose-dependent manner. On the other hand, the flowers methanolic extract and the leaves methanol, ethyl acetate, and aqueous extracts decreased the interleukin-1 beta (IL- 1β) release. It was also found that the methanol extract from the flowers and the chloroform extract from the stems of Marrubium vulgare inhibited interleukin-8 (IL-8) release. This study provides a scientific basis for the traditional use of Marrubium vulgare as an anti-inflammatory agent and for the plant to be considered as an important resource of natural antioxidants.

Investigation of Nutritional Contents, Antioxidant and Immunostimulatory Activities of Aqueous Extract from Laguncularia racemosa Leaves

2020 ◽  
Vol 10 ◽  
Author(s):  
Dayane Kelly Dias do Nascimento Santos ◽  
Cristiane Moutinho Lagos de Melo ◽  
Elivânia Maria da Silva ◽  
Vanessa Silva de Almeida ◽  
Iranildo José da Cruz Filho ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Aqueous Extract ◽  
Antioxidant Activities ◽  
Folk Medicine ◽  
Proliferation Index ◽  
Laguncularia Racemosa ◽  
High Concentration ◽  
Popular Medicine ◽  
Biological Tests ◽  
Phytochemical Profile

Background: One of the four most incident species in mangrove is the Laguncularia racemosa, plant widely used in popular medicine against inflammation and fever. Objective: Here, L. racemosa was investigated in relation to their phytochemical profile, antioxidant activity, citotoxicity, antimicrobial and immunostimulatory effect. Method: Aqueous extract was obtained from leaves of plant, its phytochemical profile was investigated through UPLC method, the antioxidant assays performed were TAA, DPPH, ABTS, nitrite and lipid peroxidation assay. Antimicrobial assays were made using standard strains. For all biological tests were used mice splenocytes and from these cell cultures were measured cytotoxicity, proliferation index and cytokines production. Results: Laguncularia racemosa leaves showed the presence of ions, proteins, carbohydrates, vitamins and high concentration of phenolic compounds. Antioxidant activities were promoted by aqueous extract, especially in DPPH and NO assays. Extract in 6 µg/mL did not induce significant cell death, stimulated the cell proliferation and the IL-4 production. Moreover, decreases of proinflammatory cytokines IFN-γ, TNF-α and IL-6 were found. Conclusion: The presence of essential nutrients, significant antioxidant activity and immune stimulation confirm the use of this plant in folk medicine against inflammation.

Antiproliferative Effects of Ocimum basilicum Methanolic Extract and Fractions, Oleanolic Acid and 3-epi-Ursolic Acid

2020 ◽  
Vol 6 (2) ◽  
pp. 134-146 ◽  
Author(s):  
Kehkashan Arshad Qamar ◽  
Ahsana Dar Farooq ◽  
Bina S. Siddiqui ◽  
Nurul Kabir ◽  
Sabira Begum
Keyword(s):  
Ursolic Acid ◽  
Oleanolic Acid ◽  
Mitotic Spindle ◽  
Immunofluorescence Microscopy ◽  
Methanolic Extract ◽  
Folk Medicine ◽  
Ocimum Basilicum ◽  
Antiproliferative Effects ◽  
Ancient Times ◽  
Mcf 7

Aims: The aim of the current study was to identify active compound(s) responsible for the antiproliferative effects of O. basilicum and explore their underlying mechanism/s. Background: Plants have been the source of medicines for the treatment of various diseases since ancient times. Ocimum basilicum (Sweet Basil, Bobai Tulsi) has been used in the folk medicine for the treatment of human liver, spleen and stomach cancers. Background: Plants have been the source of medicines for the treatment of various diseases since ancient times. Ocimum basilicum (Sweet Basil, Bobai Tulsi) has been used in the folk medicine for the treatment of human liver, spleen and stomach cancers. Objective: To emphasize the importance of O. basilicum as a potential novel non-toxic alternative to the conventional anticancer therapy. Method: O. basilicum (aerial parts) methanolic extract and fractions were screened against HT-144, MCF-7, NCI-H460 and SF-268 human cancer cell lines using sulforhodamine B assay. The more active Petroleum Ether Insoluble (PEI) fraction was fractionated into six sub-fractions (OB-1 to OB-6). Four pure compounds (3-O-methyl ursolic acid, oleanolic acid, 3-epi-ursolic acid and ursolic acid) were isolated from the more potent sub-fraction OB- 6. Triple channel immunofluorescence microscopy was employed to observe the effects of methanolic extract, PEI fraction, sub-fractions OB-5 and OB-6, 3-epi-ursolic acid and oleanolic acid on the cytoskeleton and nuclei of MCF-7 cells. Result: The methanolic extract and the PEI fraction exhibited selectively greater growth inhibition against MCF-7 cell line (TGI: 56 and 36.2 µg/ml, respectively). By using triple channel immunofluorescence microscopy, it was observed that the methanolic extract, PEI fraction, sub-fraction OB-5 and 3-epi-ursolic acid induced irregular mitotic spindle formation and slowing of mitotic progression in MCF-7 cells while sub-fraction OB-6 induced mitotic arrest in the prophase stage. F-actin aggregation was also visible in PEI fraction, subfraction OB-5 and 3-epi-ursolic acid treated MCF-7 cells. Conclusion: These results emphasize the importance of O. basilicum as a potential novel non-toxic alternative to the conventional anticancer therapy and suggest that it inhibits the growth of MCF-7 cancer cells via multiple mechanisms such as interaction with the microtubules and mitotic spindle apparatus, and F-actin aggregation.

Sign in / Sign up

Export Citation Format

Share Document