scholarly journals Microbes Associated with Freshly Prepared Juices of Citrus and Carrots

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Kamal Rai Aneja ◽  
Romika Dhiman ◽  
Neeraj Kumar Aggarwal ◽  
Vikas Kumar ◽  
Manpreeet Kaur
Keyword(s):  
Aspergillus Terreus ◽  
Heart Diseases ◽  
Agar Plate ◽  
Microbiological Quality ◽  
Fruit Juices ◽  
Human Beings ◽  
Sweet Lime ◽  
Microbiological Examination ◽  
Acid Tolerant

Fruit juices are popular drinks as they contain antioxidants, vitamins, and minerals that are essential for human being and play important role in the prevention of heart diseases, cancer, and diabetes. They contain essential nutrients which support the growth of acid tolerant bacteria, yeasts, and moulds. In the present study, we have conducted a microbiological examination of freshly prepared juices (sweet lime, orange, and carrot) by serial dilution agar plate technique. A total of 30 juice samples were examined for their microbiological quality. Twenty-five microbial species including 9 bacterial isolates, 5 yeast isolates, and 11 mould isolates were isolated from juices. Yeasts and moulds were the main cause of spoilage of juices.Aspergillus flavusandRhodotorula mucilaginosawere observed in the maximum number of juice samples. Among bacteriaBacillus cereusandSerratiawere dominant.Escherichia coliandStaphylococcus aureuswere detected in few samples.Candidasp.,Curvularia,Colletotrichum, andAcetobacterwere observed only in citrus juice samples.Alternaria,Aspergillus terreus,A. niger,Cladosporium, andFusariumwere also observed in tested juice samples. Some of the microorganisms detected in these juice samples can cause disease in human beings, so there is need for some guidelines that can improve the quality of fruit juices.

scholarly journals Microbiological Quality of Drinking Water in Sachets Sold in the City of Yaoundé in Cameroon: Tests of Sensitivity to the Antibiotics of Bacteria Isolated

2020 ◽  
Vol 8 (1) ◽  
pp. 27
Author(s):  
Nguepidjo Gilbert ◽  
Kapso Tchouankep Mireille ◽  
Ngong Ankiambom Innocent ◽  
Tonmeu Douyong Chimène Sandrine ◽  
Enoka Patrice ◽  
...  
Keyword(s):  
Drinking Water ◽  
Low Cost ◽  
Bacterial Species ◽  
Microbiological Quality ◽  
Positive Culture ◽  
Human Beings ◽  
Salmonella Spp ◽  
Cross Sectional ◽  
The City

Water is the basic drink for human beings and drinking water in sachets is very popular because of its relatively low cost and availability. The aim of this study is to determine the bacteriological profile of sachet drinking water sold in the city of Yaounde. It was a descriptive cross-sectional study covering the period from March to June 2019, carried out in the application laboratory of ETMS-Yaounde. A total of 230 samples of drinking water in sachets purchased in different markets in the city of Yaounde were analyzed using Mac Conkey's flooding method. The identification was done on the API 20 E Gallery and the susceptibility test on Mueller Hinton media. The size of the sample was 230 packaged sachet drinking water and 213 of the 230 revealed 92% of positive culture of germs, and only 17 samples gave a negative culture, at a percentage of 8% of isolated germs. The isolated bacterial species and their respective abundances in samples were Enterobacter gergoviae (3%), Klebsiella pneumoniae (5%), Proteus mirabilis (5%), Serratia fonticola (5%), Salmonella choler arizonae cloacae (8%), Salmonella spp. (8%), Enterobacter cloacae (10%), Staphylococcus aureus (10%), Pseudomonas aeruginosa (18%) and Staphylococcus epidermidis (28%). All these tested germs were resistant to Amoxicillin and Erythromycin and 70% of tested germs were sensitive to Gentamycin. Overall, the results revealed poor microbiological quality of these waters. This exposes consumers to health risks, and it is important to inform and sensitize consumers about the risks involved, to educate producers and to control their activities by the health services.

scholarly journals Chemical and Microbiological Quality of Traditional and Industrial Lime Juice Produced in Kashan, Iran

2019 ◽  
Author(s):  
Ayda Arian ◽  
Elahe Alizadeh ◽  
Navid Mazroii ◽  
Reza Sharafati Chaleshtori
Keyword(s):  
Nutritive Value ◽  
Microbiological Quality ◽  
Cross Sectional Study ◽  
National Standard ◽  
Cross Sectional ◽  
Lime Juice ◽  
Significant Difference ◽  
Soluble Solid ◽  
Acid Tolerant

Background: Lime juice is a nutritious drink, which is generally consumed for its' refreshing properties, nutritive value, vitamin content, and health benefits. Therefore, the chemical and microbiological quality of the traditional and industrial lime juice produced in Kashan city was assessed. Methods: In this descriptive cross-sectional study, a total of 106 samples were collected and screened for total soluble solid (TSS), pH, acidic value, protein content, mold, yeast, and count of acid-tolerant bacteria according to the Institute of Standards and Industrial Research of Iran (ISIRI). Results: Of the total samples, 66 samples (62.26%) were within the Iran’s national standard range. Most samples that did not meet the national standard requirements, were related to traditional samples (70.24%) compared to industrial samples (31.82%, P < 0.001). No significant difference was found between pH and protein of traditional and industrial lime juice samples (P > 0.05), while TSS and acidic values in traditional lime juices were less than those of the industrial lime juice (P < 0.05). Additionally, the mold and yeast contaminations in traditional lime juices were more than industrial lime juices (P < 0.05). Conclusion: In overall, traditional lime juice samples had the most microbial and chemical contaminations. It is necessary to increase the regular monitoring by relevant organizations over quality of the produced lime juices.

scholarly journals Microbiological Quality Assessment of Commercially and Laboratory Prepared Orange Juice

2019 ◽  
pp. 19-27
Author(s):  
Obasi B. C. ◽  
Whong C. M. Z. ◽  
Ameh J. B. ◽  
Ella E. E.
Keyword(s):  
Orange Juice ◽  
Bacterial Load ◽  
Microbiological Quality ◽  
Bacterial Isolates ◽  
Cell Counts ◽  
Trichoderma Sp ◽  
Microbiological Examination ◽  
Acid Tolerant ◽  
Aspergillus Sp ◽  
Total Yeast

Orange juice has become one of the most widely accepted natural beverages due to its invigorating flavours, source of quick energy in the form of natural sugars and its nutrient which support the growth of acid tolerant bacteria, yeasts and moulds. Present investigation was conducted to study the microbiological examination of commercially and freshly prepared orange juices. Freshly prepared orange juices were pasteurised, carbonated, concentrated and chemically treated with a preservative (sodium metabisulphite) and stored under, freezer, refrigeration and ambient temperature for 90 days. The pH ranged from 3.0 to 4.5 for the commercially, fresh and stored samples respectively. The total bacterial load ranged from 1.6x105 to 3.6x106 and the total yeast cell counts ranged from 1.7x104 to 4.8x106 cfu/ml for the commercially, freshly and stored samples. Twenty-two microbial species including 5 bacterial isolates, 6 yeast isolates and 11 isolates of mould were isolated from the orange juices. The bacterial isolates identified from the samples were Bacillus megaterium, Bacillus cereus, Bacillus pantothenticus, Bacillus aeruginosa and Escherichia coli. The fungal isolates were Saccharomyces cerevisiae, Saccharomyces rouxii, Saccharomyces telluris, Blastomyces sp, Aspergillus sp, Mycelia sp, Chrysosporium sp, and Trichoderma sp. Among the bacterial isolates, Bacillus sp was the predominant, while the fungi Saccharomyces sp and Mycelia sp were the predominant fungi and accounted for five out of 11 isolates. The isolates of bacteria, yeasts and moulds appear to be persistent throughout the period of this study and could be used as indicators of microbial quality. A safe microbial load and reduction in contamination of orange juice /fruit juices can be achieved by combination of processing methods such as chemical preservatives with pasteurisation,concentration with carbonation under controlled microbiological environments such as freezer and refrigeration temperatures.

scholarly journals Microbial Quality of Selected Commercial Fresh Fruit Juices Sold in Mysore City

2014 ◽  
Vol 8 ◽  
pp. 30-34 ◽  
Author(s):  
S Divyashree ◽  
Prakash Jamuna ◽  
N Brabahavanthis
Keyword(s):  
Chemical Properties ◽  
Poor Quality ◽  
Fruit Juices ◽  
Microbial Quality ◽  
Public Places ◽  
Pineapple Juice ◽  
Quality Of Water ◽  
Sweet Lime ◽  
Physico Chemical

Fruit juices are common man’s beverages and sold at public places. Since they are known for carrying heavy microbial load, the present investigation was undertaken to enumerate and identify the microorganisms in fruit juices (sweet lime, orange and pineapple) selected from three different street vended shops (source A, B and C) in Mysore. The juices were analyzed for the microbial quality for type of organisms and number of colonies by serial dilution technique, pour plate method,gram’s staining method and staining for fungi; and physico-chemical properties. Results showed that the pineapple juice from two sources was highly contaminated with bacterial pathogens (25×104CFU/mland 20×104CFU/ml). All samples were contaminated with faecal streptococci. In source-B and C, all juices showed high counts of fungal colonies (Aspergillus, Penicillium, Fusarium and yeast). It is contended that contamination was mainly due to poor quality of water used for dilution as well as prevailing unhygienic conditions related to washing of utensils and maintenance of the premises. The result showed the poor hygienic quality of street vended fruit juices and the possible risk to the consumers. DOI: http://dx.doi.org/10.3126/jfstn.v8i0.11746 J. Food Sci. Technol. Nepal, Vol. 8 (30-34), 2013

scholarly journals Microbiological analysis of pasteurized and fresh fruit juice sold in Rawalpindi of Pakistan

2013 ◽  
Vol 48 (3) ◽  
pp. 185-192 ◽  
Author(s):  
SA Batool ◽  
SS Tahir ◽  
N Rauf ◽  
R Kalsoom
Keyword(s):  
Fruit Juice ◽  
Microbiological Quality ◽  
Total Coliform ◽  
Viable Count ◽  
Fruit Juices ◽  
Local Market ◽  
Microbiological Analysis ◽  
E Coli ◽  
Bacteria And Fungi

Freshly prepared and pasteurized fruit juices sold by vendors in local market of Rawalpindi city from 10 locations were analyzed for the microbiological quality. Total viable count (TVC), total coliform, faecal coliform, molds and the presence of pathogenic microorganisms such as E. coli, Pseudomonas, Staphylococcus aureus, Salmonella, and fungi like Aspergillus, Pencillum, Rhizopus were determined .In open fruit juices available in city were highly contaminated with bacteria and fungi. E. coli, Salmonella, Staphylococcus, and Pseudomonas were isolated with different frequency. Aspergillus, Pencillum and Rhizopus were also found in juices especially Aspergillus was with high percentages. The pasteurized juices have less contamination as compared to the fresh juices samples. The number and type of microorganisms recovered from the freshly squeezed fruit juices made them unsafe for drinking. The results of this study demonstrate the unhygienic quality of popular types of market vended freshly squeezed fruit juices and their risk to the consumers. DOI: http://dx.doi.org/10.3329/bjsir.v48i3.17329 Bangladesh J. Sci. Ind. Res. 48(3), 185-192, 2013

scholarly journals Influence of physicochemical parameters on storage stability: Microbiological quality of fresh unpasteurized fruit juices

2017 ◽  
Vol 5 (6) ◽  
pp. 1098-1105 ◽  
Author(s):  
Phoebe P. Kaddumukasa ◽  
Samuel M. Imathiu ◽  
Julius M. Mathara ◽  
Jesca L. Nakavuma
Keyword(s):  
Physicochemical Parameters ◽  
Storage Stability ◽  
Microbiological Quality ◽  
Fruit Juices

scholarly journals Assessment of the microbiological quality of beverages sold in collective cafes on the campuses of the University of Abomey-Calavi, Benin Republic

2020 ◽  
Author(s):  
Gwladys S. Komagbe ◽  
Philippe Sessou ◽  
François Dossa ◽  
Prudencio Sossa-Minou ◽  
Bernard Taminiau ◽  
...  
Keyword(s):  
Pathogenic Bacteria ◽  
Compliance Rate ◽  
Microbiological Quality ◽  
Resistant Bacteria ◽  
Human Beings ◽  
Salmonella Spp ◽  
Potential Health ◽  
Antibiotic Susceptibility Test ◽  
Aerobic Flora

Fresh juices are highly nutritious foods for human beings, but the inability to observe requirements for their preparation, packaging and storage subjects them to microbial contamination which poses a potential health risk to consumers. The purpose of this study was to evaluate the microbiological quality of beverages sold within the cafes of the campuses of Abomey-Calavi University (Benin). A survey carried out among beverage vendors showed that the sources of contamination were uncontrolled and the raw materials used were of questionable quality as the operators lacked good hygienic practices. Thus, the microbial quality of forty-five samples of four types of beverages sold in these cafes was investigated for mesophilic aerobic flora, Coliforms, Escherichia coli, Staphylococcus aureus, sulfate-reducing anaerobic spores, fungal flora and Salmonella spp. using standardized methods. Then, molecular studies identified the pathogenic strains isolated from the beverages. An antibiotic susceptibility test was performed on the strains identified for the detection of multi-resistant bacteria. These analyses revealed a non-compliance rate of 100% in the analyzed samples. The indicators that caused this non-compliance in the samples were mesophilic aerobic flora, coliforms and fungi. In addition, 85.7% of the samples contained other Enterobacteriaceae including Klebsiella pneumoniae, Morganella morganii, Kluyvera georgiana, Citrobacter murliniae, Yersinia intermedia. While the non-compliance rates of the samples for Salmonella spp and E.coli were 4.4% each, the non-compliance rate for S. aureus was 2.2% with the presence of sometimes multi-resistant pathogenic bacteria. Sellers' awareness of good hygiene practices is important for improving the quality of food sold.

scholarly journals Kualitas air minum isi ulang pada depot air minum di Kabupaten Manokwari Selatan

Cassowary ◽  
2020 ◽  
Vol 3 (1) ◽  
pp. 61-71
Author(s):  
Awliya Nur Marhamah ◽  
Budi Santoso ◽  
Budi Santoso
Keyword(s):  
Drinking Water ◽  
Reverse Osmosis ◽  
Microbiological Quality ◽  
Drinking Water Treatment ◽  
Quality Standard ◽  
Special Treatment ◽  
Human Beings ◽  
Water Treatment Process ◽  
Living Things

Water is a chemical compound that is very important for the life of human beings and other living things. Refill water is water that undergoes special treatment, among others, the process of chlorination, aeration, filtration and irradiation with ultraviolet light. The objectives of this study were to determine the quality of refill drinking water produced by refill drinking water depots in South Manokwari Regency based on physical, chemical and microbiological variables. The descriptive method was used in this study. Samples of refill drinking water were collected from 6 depots located in South Manokwari Regency.   Water samples are taken to the laboratory for chemical, physical and microbiological quality analyzes. Good quality refill drinking water at the kiosk is drinking water produced by depots B and F, while drinking water produced from depot E was quite good.. The refill drinking water treatment process was carried out using the reverse osmosis method, a combination of the reverse osmosis and ultraviolet methods. The quality of refill drinking water in depots B, D, and F meets the quality standard requirements based on Permenkes 2010. E. coli population contained in refill water in depots A, C and F after storage in gallons for 3 days was above the quality standard requirements so it must be boiled before being consumed by humans.

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