scholarly journals Intake of Red Wine in Different Meals Modulates Oxidized LDL Level, Oxidative and Inflammatory Gene Expression in Healthy People: A Randomized Crossover Trial

2014 ◽  
Vol 2014 ◽  
pp. 1-9 ◽  
Author(s):  
Laura Di Renzo ◽  
Alberto Carraro ◽  
Roberto Valente ◽  
Leonardo Iacopino ◽  
Carmen Colica ◽  
...  
Keyword(s):  
Gene Expression ◽  
Red Wine ◽  
Positive Impact ◽  
Oxidized Ldl ◽  
Antioxidant Genes ◽  
Beneficial Effects ◽  
Inflammatory Conditions ◽  
Before And After ◽  
First Time ◽  
Positive Effect

Several studies have found that adherence to the Mediterranean Diet, including consumption of red wine, is associated with beneficial effects on oxidative and inflammatory conditions. We evaluate the outcome of consumption of a McDonald’s Meal (McD) and a Mediterranean Meal (MM), with and without the additive effect of red wine, in order to ascertain whether the addition of the latter has a positive impact on oxidized (ox-) LDL and on expression of oxidative and inflammatory genes. A total of 24 subjects were analyzed for ox-LDL, CAT, GPX1, SOD2, SIRT2, and CCL5 gene expression levels, before and after consumption of the 4 different meal combinations with washout intervals between each meal. When red wine is associated with McD or MM, values of ox-LDL are lowered (P<0.05) and expression of antioxidant genes is increased, while CCL5 expression is decreased (P<0.05). SIRT2 expression after MM and fasting with red wine is significantly correlated with downregulation of CCL5 and upregulation of CAT (P<0.001). GPX1 increased significantly in the comparison between baseline and all conditions with red wine. We highlighted for the first time the positive effect of red wine intake combined with different but widely consumed meal types on ox-LDL and gene expression.Trial Registration. This trial is registered with ClinicalTrials.govNCT01890070.

scholarly journals Vitrification alters rabbit foetal placenta at transcriptomic and proteomic level

Reproduction ◽  
2014 ◽  
Vol 147 (6) ◽  
pp. 789-801 ◽  
Author(s):  
M D Saenz-de-Juano ◽  
F Marco-Jimenez ◽  
B Schmaltz-Panneau ◽  
E Jimenez-Trigos ◽  
M P Viudes-de-Castro ◽  
...  
Keyword(s):  
Gene Expression ◽  
Birth Weight ◽  
Protein Expression ◽  
Strong Evidence ◽  
Foetal Development ◽  
Crucial Step ◽  
Metabolic Alteration ◽  
Gene And Protein Expression ◽  
Before And After ◽  
First Time

Although numerous studies have demonstrated that cryopreservation alters gene expression, less is known about those embryos that implanted successfully and continued in gestation. To raise the question of the neutrality of this technique, we examine the effects of vitrification through gestation in rabbit before and after the implantation. We monitored the distribution of losses of 569 vitrified morulae, observing that embryos which reach the last pre-implantatory stage are able to implant. However, we found that not all implanted embryos had the ability to continue with their gestation. The results reveal that vitrification decreased foetus and maternal placenta weights at mid-gestation, but led to a higher offspring birth weight. A novel finding is that while no differences in gene expression were detected in pre-implantatory embryos at day 6, vitrification affects a gene and protein expression in the placenta at day 14. Our results for first time reveal strong evidence of modifications in implanted embryos subjected to vitrification, suggesting that the crucial step that vitrified embryos must overcome is the placenta formation. On the basis of these findings, our work leaves the question open as to whether the effects we observed that cause vitrification during foetal development could give rise to some type of physiological or metabolic alteration in adulthood.

scholarly journals Innovative outcome through exploration and exploitation – Enablers, barriers and industrial property

2021 ◽  
Vol ahead-of-print (ahead-of-print) ◽  
Author(s):  
Nancy Vargas ◽  
M. Begoña Lloria ◽  
Addisson Salazar ◽  
Luis Vergara
Keyword(s):  
Positive Impact ◽  
Exploration And Exploitation ◽  
Economic Sectors ◽  
Industrial Property ◽  
Content Type ◽  
Close Relationship ◽  
Relationship Model ◽  
High Level ◽  
First Time ◽  
Positive Effect

PurposeThis research aims to study the effect of R&D (research and development) enablers and barriers as well as industrial property on exploration, their influence on exploitation and finally the possible impact on innovative outcome (IO) as a result variable. The IO can be defined as the orientation towards new or improved products, services and processes, as well as towards penetration and greater market share, which the company has obtained as a result of innovative processes.Design/methodology/approachFor this purpose, a new relationship model is defined, which is empirically contrasted in a quantitative study. We use a sample of large firms from different economic sectors with a high level of investment in R&D.FindingsThe results indicate a close relationship between exploration and exploitation processes, as well as a positive impact on the innovative outcome. Moreover, the type of relationship that R&D enablers and barriers have with exploration is demonstrated and the lack of a positive effect of industrial property on exploration.Practical implicationsThese results may lead to new markets opening up and the creation or improvement of new products, services or processes in diverse sectors of highly innovative firms.Originality/valueThis research aims to study the effect of R&D enablers and barriers and industrial property on learning flows and, finally, the possible impact on the innovative outcome. A new theoretical model of relationships is defined, and it is the first time that it is empirically tested.研究目的本研究旨在探討研究與開發的推動者、研發的障礙和工業產權對探索與開發的影響，並最終探究出其可能對作為結果變項的創新成果帶來的影響。創新成果可解釋為邁向新的或改良的產品，服務和流程的定向，以及邁向滲透和更大的市場份額的定向，而這更大的市場份額是企業的創新流程所帶來的。研究的設計/方法/理念為達研究目的，我們解說了一個新的關聯模型, 並使用於一項量化研究，進行以實驗為依據的對比。我們使用的樣本，包括來自不同經濟領域，並於研發投放高水平投資的大企業。研究結果研究結果顯示，探索與開發是兩個關係密切的流程；研究結果亦顯示對創新成果的影響是正面的。而且，結果展示了研發推動者、研發障礙兩者與探索的關聯，以及工業產權對探索是欠缺正面作用的。實際的意義研究結果或會帶來新市場的開拓，以及在不同領域、高度創新的企業內，帶來新的產品，服務與流程的創造和改良。研究的原創性和價值本研究旨在探討研發推動者，研發障礙和工業產權對學習心流的影響，並最終探究出其對創新成果可能帶來的影響。研究解說了一個新的、純理論的關聯模型，而這個模型於此是首次憑實驗而被測試的。

scholarly journals A Systematic Review on Natural Antioxidant Properties of Resveratrol

2018 ◽  
Vol 13 (9) ◽  
pp. 1934578X1801300 ◽  
Author(s):  
Carmela Colica ◽  
Maja Milanović ◽  
Nataša Milić ◽  
Vincenzo Aiello ◽  
Antonino De Lorenzo ◽  
...  
Keyword(s):  
Fast Food ◽  
Red Wine ◽  
Positive Impact ◽  
Antioxidant Properties ◽  
Epidemiological Studies ◽  
Scientific Data ◽  
Plant Metabolites ◽  
Beneficial Effects ◽  
Pubmed Database ◽  
Wide Range

Polyphenols, including anthocyanins, flavonoids and stilbenes, which constitute one of the most abundant and ubiquitous groups of plant metabolites, are an integral part of the human diet. Resveratrol (3,5,4'-trihydroxystilbene), a naturally occurring polyphenol produced by some plants as a self-defence agent, has an antifungal activity. Resveratrol has been found in some plants (such as grapevine, pine and peanuts) and is considered to have beneficial effects also on human health. The number of studies on resveratrol greatly increased in PubMed database since 1997, after the anticancer effect of this molecule was first reported. The interest in resveratrol in grape was originally sparked by epidemiological studies indicating an inverse relationship between long-standing moderate consumption of red wine and the risk of coronary heart disease; this effect has been ascribed to resveratrol, which possesses diverse biochemical and physiological properties, including antiplatelet and anti-inflammatory proprieties, and provides a wide range of health benefits ranging from chemoprevention to cardioprotection. Recently, resveratrol has been described as an anti-aging compound. The consumption of resveratrol (red wine) together with a Mediterranean diet or a fast-food meal (“McDonald'sMeal”) had a positive impact on oxidized (ox-) LDL and on the expression of oxidative and inflammatory genes. Therefore, this review summarized the most important scientific data about healing and preventive potential of resveratrol, acting as cardioprotective, neuroprotective, chemopreventive and antioxidant agent.

scholarly journals Alteration of STAT3 gene expression in psoriasis treatment

2020 ◽  
pp. 71-74
Author(s):  
V. V. Sobolev ◽  
E. V. Denisova ◽  
I. M. Korsunskaya
Keyword(s):  
Gene Expression ◽  
Laser Radiation ◽  
Short Wave ◽  
Pcr Analysis ◽  
Low Level ◽  
Psoriasis Treatment ◽  
Low Level Laser ◽  
Inflammatory Conditions ◽  
Level Laser ◽  
Before And After

Introduction. Psoriasis is a typical complex multigenic and multifactorial disease with heterogeneous genetic heredity, which requires the interaction of genes both with each other and with environmental factors. STAT3 (Signal Transducer and Activator of Transcription 3) has only recently been considered a key player in the development and pathogenesis of psoriasis and psoriatic inflammatory conditions.Aim of the study. To study the expression of the STAT3 gene in the affected part of the skin of psoriasis patients in relation to the visually unaffected part. To study the change in the STAT3 gene expression level in psoriasis-affected skin as compared to nonaffected skin in patients before and after treatment with low-level laser radiation at a wavelength of 1.27 pm.Materials and methods. The study involved 12 psoriasis patients. Biopsies from the unaffected skin were taken at a distance of about 3 cm from the affected skin. Real-time PCR analysis was performed.Results and discussion. The expression of the STAT3 gene was quantitatively measured using RT-PCR in the affected part of the skin of psoriasis patients compared to the visually unaffected part of the skin of the same patients before and after treatment with low-level laser radiation with a wavelength of 1.27 gm (short-wave infrared). As a result of the study, an increase in the expression of the STAT3 gene in the affected part of the skin of psoriasis patients of an average of 3.96 ± 2 times was experimentally shown. A decrease in gene expression was observed in psoriasis affected skin compared to samples of non-affected areas. After treatment of patients with low-level laser radiation, a significant reduction in the expression of the overexpressed STAT3 gene to 1.75 ± 0.5 times was observed.Conclusions. The transcription activity of the STAT3 gene can be an indicator of the efficacy of psoriasis treatment at the molecular level and can also be a new therapeutic target. 

scholarly journals Aquatic Exercise to Treat Pregnancy Related Issues in Pregnant Women: A Systematic Review

2020 ◽  
Vol 6 (2) ◽  
pp. 391-400
Author(s):  
Asif Ali ◽  
Muhammad Azam ◽  
Zargham ul Hassan Askry
Keyword(s):  
Pregnant Women ◽  
Psychological Factors ◽  
Positive Impact ◽  
Labor Pain ◽  
Assessment Procedure ◽  
Sleep Health ◽  
Beneficial Effects ◽  
Physical Problems ◽  
Health Related ◽  
Positive Effect

Background: Wide ranges of physiological changes occur as a result of pregnancy in women that, in certain cases, may cause variety of psychological and physical problems in pregnant women.  Aquatic exercises believed to be safe for pregnant women and believed to foster positive effect on psychophysical health. Current health care setting and nursing emphasizes on evidence based approaches when developing and implementing interventions to address health related issues of pregnant women. Purpose: This review sought to synthesize qualitatively the existing research evidences concerning association between aquatic exercises and psychophysical problems in pregnant women. Methods: A 1989 records were yielded as result of conducting a systematic search in nine research data bases. After undergoing rigorous screening and assessment procedure of eligibility, and quality appraisal, a total of seventeen research articles were ultimately selected to include in this review. Result: Out of seventeen admissible studies, ten studies found that aquatic exercises exerts positive impact to decreases variety of physical discomforts such as fatigue, pelvic pain, labor pain, and edema. The remaining seven studies presented beneficial effects of aquatic exercises for psychological factors such as body image, stress, depression, feelings, mood, distress, and anxiety in pregnant women. Moreover, among the seventeen studies, only two studies showed effectiveness of aquatic exercises to manage body weight and two studies found improvement in sleep during pregnancy.    Conclusion: This review found some evidence in support of the efficacy of aquatic exercises to reduce physical discomfort and improve psychological factors in pregnant women. More studies essential to reach stronger conclusion. The existing evidence concerning effectiveness of this intervention for weight management and sleep health during pregnancy is insufficient.

scholarly journals Regulation of Nrf2 by Mitochondrial Reactive Oxygen Species in Physiology and Pathology

Biomolecules ◽  
2020 ◽  
Vol 10 (2) ◽  
pp. 320 ◽  
Author(s):  
Shuya Kasai ◽  
Sunao Shimizu ◽  
Yota Tatara ◽  
Junsei Mimura ◽  
Ken Itoh
Keyword(s):  
Gene Expression ◽  
Reactive Oxygen Species ◽  
Physiological Stress ◽  
Reactive Oxygen ◽  
Negative Regulator ◽  
Endurance Capacity ◽  
Oxygen Species ◽  
Antioxidant Genes ◽  
Beneficial Effects ◽  
Quantity Control

Reactive oxygen species (ROS) are byproducts of aerobic respiration and signaling molecules that control various cellular functions. Nrf2 governs the gene expression of endogenous antioxidant synthesis and ROS-eliminating enzymes in response to various electrophilic compounds that inactivate the negative regulator Keap1. Accumulating evidence has shown that mitochondrial ROS (mtROS) activate Nrf2, often mediated by certain protein kinases, and induce the expression of antioxidant genes and genes involved in mitochondrial quality/quantity control. Mild physiological stress, such as caloric restriction and exercise, elicits beneficial effects through a process known as “mitohormesis”. Exercise induces NOX4 expression in the heart, which activates Nrf2 and increases endurance capacity. Mice transiently depleted of SOD2 or overexpressing skeletal muscle-specific UCP1 exhibit Nrf2-mediated antioxidant gene expression and PGC1α-mediated mitochondrial biogenesis. ATF4 activation may induce a transcriptional program that enhances NADPH synthesis in the mitochondria and might cooperate with the Nrf2 antioxidant system. In response to severe oxidative stress, Nrf2 induces Klf9 expression, which represses mtROS-eliminating enzymes to enhance cell death. Nrf2 is inactivated in certain pathological conditions, such as diabetes, but Keap1 down-regulation or mtROS elimination rescues Nrf2 expression and improves the pathology. These reports aid us in understanding the roles of Nrf2 in pathophysiological alterations involving mtROS.

scholarly journals Treating Stress-Related Pain with the Flotation Restricted Environmental Stimulation Technique: Are There Differences between Women and Men?

2009 ◽  
Vol 14 (4) ◽  
pp. 293-298 ◽  
Author(s):  
Sven Å Bood ◽  
Anette Kjellgren ◽  
Torsten Norlander
Keyword(s):  
Sex Differences ◽  
Pain Treatment ◽  
Muscle Tension ◽  
Beneficial Effects ◽  
Before And After ◽  
Environmental Stimulation ◽  
Restricted Environmental Stimulation ◽  
First Time ◽  
Stimulation Technique ◽  
Experimentally Induced

The aim of the present study was to explore, for the first time, sex differences among patients diagnosed with stress-related pain before and after flotation restricted environmental stimulation technique (REST) treatment, delivered 12 times during seven weeks. The present study included 88 patients (69 women, 19 men) from three different studies (post hoc analysis). They had been diagnosed by a physician as having chronic stress-related muscle tension pain. The analyses indicated that the flotation-REST treatment had beneficial effects on stress, anxiety, depression, sleep quality and pain and that there were few sex differences. Women were more depressed than men before treatment, but after treatment there was no difference between sexes. However, there was a sex difference in the ability to endure experimentally induced pain, suggesting that men exhibited greater endurance both before and after the flotation-REST treatment. The results also showed, for the first time, that both sexes improved their ability to endure experimentally induced pain (higher scores for upper pain threshold) following the successful flotation-REST pain treatment.

scholarly journals Positive Impact of Natural Deep Eutectic Solvents on the Biocatalytic Performance of 5-Hydroxymethyl-Furfural Oxidase

Catalysts ◽  
2020 ◽  
Vol 10 (4) ◽  
pp. 447 ◽  
Author(s):  
Gonzalo de Gonzalo ◽  
Caterina Martin ◽  
Marco W. Fraaije
Keyword(s):  
Dicarboxylic Acid ◽  
Carbonyl Compounds ◽  
Positive Impact ◽  
Deep Eutectic Solvents ◽  
Redox Enzymes ◽  
Beneficial Effects ◽  
Hydroxymethyl Furfural ◽  
Natural Deep Eutectic Solvents ◽  
The Stability ◽  
Positive Effect

Deep eutectic solvents (DESs) have been applied as cosolvents in various biocatalytic processes during recent years. However, their use in combination with redox enzymes has been limited. In this study, we have explored the beneficial effects of several DES as cosolvents on the performance of 5-hydroxymethylfurfural oxidase (HMFO), a valuable oxidative enzyme for the preparation of furan-2,5-dicarboxylic acid (FDCA), and other compounds, such as carbonyl compounds and carboxylic acids. The use of natural DESs, based on glucose and fructose, was found to have a positive effect. Higher conversions are obtained for the synthesis of several oxidized compounds, including FDCA. Depending on the type of DES, the stability of HMFO could be significantly improved. As the use of DES increases the solubility of many substrates while they only mildly affect dioxygen solubility, this study demonstrates that biocatalysis based on HMFO and other redox biocatalysts can benefit from a carefully selected DES.

scholarly journals In vitro glucuronidation of trans-Piceid and trans-Piceatannol by human liver microsomes

OENO One ◽  
2008 ◽  
Vol 42 (4) ◽  
pp. 241
Author(s):  
Caroline Henry-Vitrac ◽  
Thomas Richard ◽  
Alexis Desmoulière ◽  
Jean-Pierre Monti ◽  
Jean-Michel Mérillon ◽  
...  
Keyword(s):  
Human Liver ◽  
Red Wine ◽  
Biological Activities ◽  
Liver Microsomes ◽  
Human Liver Microsomes ◽  
Beneficial Effects ◽  
Naturally Occurring ◽  
Phenolic Substances ◽  
First Time

<p style="text-align: justify;"><strong>Aims</strong> : The aim of the present investigation was to establish glucuronidation of trans-resveratrol derivates in the liver. Stilbenes are naturally occurring polyphenolic compounds which have been reported to have potential preventive activities in human diseases. Trans-stilbenes, mainly found in grapes and red wine, are important in terms of biological activities. However, little is known about the metabolism of these compounds in human.</p><p style="text-align: justify;"><strong>Methods and results</strong> : The glucuronoconjugation of stilbenes was investigated using human liver microsomes and the structure of new metabolites was characterized by LC-MS and proton NMR. For the first time, the structure of the metabolites of trans-piceid and trans-piceatannol was established. The reaction led to the formation of two glucuronides for trans-piceid and three for trans-piceatannol.</p><p style="text-align: justify;"><strong>Significance and impact of study</strong>: This study is of particular relevance since the phenolic substances of red wine (especially stilbenes) might be responsible for the potential beneficial effects of moderate and regular wine consumption.</p>

Sign in / Sign up

Export Citation Format

Share Document