Ubiquinol Improves Symptoms in Children with Autism

Background. Autism is a spectrum of neurodevelopmental disorders with manifestation within 3 years after birth. Manifestations of autism include behavior problems (hyperactivity, toys destruction, self-harm, and agression) and sleep and eating disorders. Etiology of autism is poorly understood. Oxidative stress and antioxidants can participate in pathobiochemical mechanisms of autism.Methods. Twenty-four children, aged 3–6 years, with autism according to the DSM IV criteria and using CARS were included in the study. Concentrations ofCoQ10-TOTAL,γ- andα-tocopherol,β-carotene, and lipid peroxidation were determined in plasma before and after three months of supportive therapy with ubiquinol at a daily dose2×50 mg. Data on behavior of the children were collected from parents at the same time.Results. Ubiquinol supportive therapy improved symptoms in children with autism, as communication with parents (in12%), verbal communication (in21%), playing games of children (in42%), sleeping (in34%), and food rejection (in17%), withCoQ10-TOTALplasma level above2.5 μmol/L.Conclusions. Beneficial effect of ubiquinol in children with autism has been demonstrated for the first time. We assume that plasma concentration ofCoQ10-TOTALand lipid peroxidation could be used as relevant biomarkers of ubiquinol supportive therapy.

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Lipid peroxidation-derived aldehydes and oxidative stress in the failing heart: role of aldose reductase

AJP Heart and Circulatory Physiology ◽

10.1152/ajpheart.00592.2002 ◽

2002 ◽

Vol 283 (6) ◽

pp. H2612-H2619 ◽

Cited By ~ 54

Author(s):

Sanjay Srivastava ◽

Bysani Chandrasekar ◽

Aruni Bhatnagar ◽

Sumanth D. Prabhu

Keyword(s):

Oxidative Stress ◽

Lipid Peroxidation ◽

Aldose Reductase ◽

Transcriptional Activation ◽

Pressure Rise ◽

Oxidative Injury ◽

Left Ventricular ◽

Protein Levels ◽

Failing Heart ◽

Before And After

Lipid peroxidation-derived aldehydes (LP-DA) can propagate oxidative injury and are detoxified by the aldose reductase (AR) enzyme pathway in myocardium. Whether there are alterations in the AR axis in heart failure (HF) is unknown. Sixteen instrumented dogs were studied before and after either 24 h or 4 wk of rapid left ventricular (LV) pacing (early and late HF, respectively). Six unpaced dogs served as controls. In early HF, there was subtle depression of LV performance (maximum rate of LV pressure rise, P < 0.05 vs. baseline) but no chamber enlargement, whereas in late HF there was significant ( P < 0.05) contractile depression and LV dilatation. Oxidative stress was increased at both time points, indexed by tissue malondialdehyde, total glutathione, and free C6–C9 LP-DA ( P < 0.025 vs. control). AR protein levels and activity decreased progressively during HF ( P < 0.025 early/late HF vs. control); however, AR mRNA expression decreased only in late HF ( P < 0.005 vs. early HF and control). DNA binding of tonicity-responsive enhancer binding protein (TonEBP, a transcriptional regulator of AR) paralleled AR mRNA, declining >50% in late HF ( P < 0.025 vs. control). We conclude that AR levels and attendant myocardial capacity to detoxify LP-DA decline during the development of HF. In early HF, decreased AR occurs due to a translational or posttranslational mechanism, whereas in late HF reduced TonEBP transcriptional activation and AR downregulation contribute significantly. Reduced AR-mediated LP-DA metabolism contributes importantly to LP-DA accumulation in the failing heart and thus may augment chronic oxidative injury.

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Exposure of β H-crystallin to hydroxyl radicals enhances the transglutaminase-susceptibility of its existing amine-donor and amine-acceptor sites

Biochemical Journal ◽

10.1042/bj2950399 ◽

1993 ◽

Vol 295 (2) ◽

pp. 399-404 ◽

Cited By ~ 21

Author(s):

P J T A Groenen ◽

M Seccia ◽

R H P H Smulders ◽

E Gravela ◽

K H Cheeseman ◽

...

Keyword(s):

Oxidative Stress ◽

Hydroxyl Radicals ◽

Primary Amine ◽

Lens Crystallins ◽

Acceptor Substrate ◽

Transglutaminase Activity ◽

Donor Substrate ◽

Before And After ◽

First Time ◽

Oxidative Attack

beta H-crystallin was exposed to radiolytically generated hydroxyl radicals at defined radical concentrations, and its capacity to act as an amine-acceptor substrate and as an amine-donor substrate for transglutaminase were investigated. [14C]Methylamine was used as a probe for labelling amine-acceptor sites; a novel biotinylated hexapeptide was used to label amine-donor sites. The results demonstrate that both primary amine incorporation and hexapeptide incorporation by transglutaminase are considerably increased after oxidative attack on the crystallin. The identity of the labelled subunits was established, and it is shown that, in both cases, this increased incorporation is not due to the production of new substrates, but that the existing incorporation sites become more susceptible. Moreover, using the newly developed probe, we could identify, for the first time, the major crystallin subunits active as amine-donor substrates (both before and after treatment) to be beta B1-, beta A3- and beta A4-crystallin. These data support the proposal that oxidative stress and transglutaminase activity may be jointly involved in the changes found in lens crystallins with age and in the development of cataract.

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Habitual exercise program protects murine intestinal, skeletal, and cardiac muscles against aging

Journal of Applied Physiology ◽

10.1152/japplphysiol.00417.2005 ◽

2005 ◽

Vol 99 (4) ◽

pp. 1569-1575 ◽

Cited By ~ 47

Author(s):

Eloi F. Rosa ◽

Antonio C. Silva ◽

Silvia S. M. Ihara ◽

Oswaldo A. Mora ◽

Jeannine Aboulafia ◽

...

Keyword(s):

Oxidative Stress ◽

Lipid Peroxidation ◽

Protective Effect ◽

Exercise Program ◽

Aging Effects ◽

Cross Sectional ◽

Habitual Exercise ◽

Cardiac Muscles ◽

Effects Of Aging ◽

First Time

Aging and aerobic exercise are two conditions known to interfere with health and quality of life, most likely by inducing oxidative stress to the organism. We studied the effects of aging on the morphological and functional properties of skeletal, cardiac, and intestinal muscles and their corresponding oxidative status in C57BL/6 mice and investigated whether a lifelong moderate exercise program would exert a protective effect against some deleterious effects of aging. As expected, aged animals presented a significant reduction of physical performance, accompanied by a decrease of gastrocnemius cross-sectional area and cardiac hypertrophy. However, most interesting was that aging dramatically interfered with the intestinal structure, causing a significant thickening of the ileum muscular layer. Senescent intestinal myocytes displayed many mitochondria with disorganized cristae and the presence of cytosolic lamellar corpuscles. Lipid peroxidation of ileum and gastrocnemius muscle, but not of the heart, increased in aged mice, thus suggesting enhanced oxidative stress. With exception of the intestinal muscle responsiveness, animals submitted to a daily session of 60 min, 5 days/wk, at 13 up to 21 m/min of moderate running in treadmill during animal life span exhibited a reversion of all the observed aging effects on intestinal, skeletal, and heart muscles. The introduction of this lifelong exercise protocol prevented the enhancement of lipid peroxidation and sarcopenia and also preserved cellular and ultracellular structures of the ileum. This is the first time that the protective effect of a lifelong regular aerobic physical activity against the deleterious effects of aging on intestinal muscle was demonstrated.

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A New Approach to Oxidative Stress and Inflammatory Signaling during Labour in Healthy Mothers and Neonates

Oxidative Medicine and Cellular Longevity ◽

10.1155/2015/178536 ◽

2015 ◽

Vol 2015 ◽

pp. 1-8 ◽

Cited By ~ 15

Author(s):

Javier Díaz-Castro ◽

Jesus Florido ◽

Naroa Kajarabille ◽

Sonia Prados ◽

Catalina de Paco ◽

...

Keyword(s):

Oxidative Stress ◽

Oxidative Damage ◽

Umbilical Cord ◽

Inflammatory Cytokines ◽

Umbilical Vein ◽

Inflammatory Signaling ◽

New Approach ◽

Before And After ◽

First Time ◽

Spontaneous Delivery

The objective of the current study was to investigate for the first time and simultaneously the oxidative stress and inflammatory signaling induced during the delivery in healthy mothers and their neonates. 56 mothers with normal gestational course and spontaneous delivery were selected. Blood samples were taken from mother (before and after delivery) both from vein and artery of umbilical cord. Lower antioxidant enzymes activities were observed in neonates compared with their mothers and lower oxidative stress in umbilical cord artery with respect to vein. There was an overexpression of inflammatory cytokines in the mother, such as IL-6 and TNF-α, and, in addition, PGE2was also increased. Neonates showed lower levels of IL-6 and TNF-αand higher values of sTNF-RII and PGE2in comparison with their mothers. Parturition increases oxidative damage in the mother, although the indicators of oxidative damage were lower in umbilical cord artery with respect to umbilical vein. The overexpression of inflammatory cytokines reveals that fetus suffers its own inflammatory process during parturition.

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Saliva Levels of 15-F2t-Isoprostane as Biomarker of Lipid Peroxidation in Autistic Children

European Journal of Inflammation ◽

10.1177/1721727x0700500304 ◽

2007 ◽

Vol 5 (3) ◽

pp. 141-144

Author(s):

A. Karamouzi ◽

D. Kovachev ◽

I. Karamouzis ◽

M. Antoniadou-Hitoglou ◽

I. Tsikoulas ◽

...

Keyword(s):

Oxidative Stress ◽

Lipid Peroxidation ◽

Biological Fluid ◽

Neurodevelopmental Disorder ◽

Children With Autism ◽

Control Group ◽

Healthy Controls ◽

Autistic Children ◽

Genetic And Environmental Factors ◽

Fluid Levels

Autism is a complex neurodevelopmental disorder that usually presents itself in early childhood and is thought to be influenced by genetic and environmental factors. Oxidative stress has been implicated in the etiology of neurological, neurodevelopment and neuropsychiatric disorders including Parkinson's and Alzheimer disease, Down's syndrome and autism. The biological fluid levels of the 15-F2t-Isoprostane (15-F2t-IsoP) are regarded as being the most significant biochemical index of oxidative stress. There are some studies that measured 15-F2t-IsoP in the plasma and urine of children with autism. However 15-F2t-IsoP has not been measured in the saliva of children with autism. This procedure is less stressful for the children with autism. The purpose of this study is to evaluate saliva levels of 15-F2t-IsoP in children diagnosed with autism. Saliva levels of 15-F2t-IsoP were determined in 18 children with autism age: 6.6±1.6 years and 21 healthy controls age: 7.5±1.4 years. Compared to the control group, the children with autism had significantly higher saliva levels of 15-F2t-IsoP. From the above-mentioned it is concluded that a) lipid peroxidative biomarker 15-F2t-IsoP is increased in autistic children, and b) saliva is the proper (optimal) biological fluid for the evaluation the lipid peroxidation in autistic children.

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Hidrox® Counteracts Cyclophosphamide-Induced Male Infertility through NRF2 Pathways in a Mouse Model

Antioxidants ◽

10.3390/antiox10050778 ◽

2021 ◽

Vol 10 (5) ◽

pp. 778

Author(s):

Roberta Fusco ◽

Angela Trovato Salinaro ◽

Rosalba Siracusa ◽

Ramona D’Amico ◽

Daniela Impellizzeri ◽

...

Keyword(s):

Oxidative Stress ◽

Lipid Peroxidation ◽

Male Infertility ◽

The Other ◽

Antioxidant Defenses ◽

Distilled Water ◽

Single Intraperitoneal Injection ◽

Strongly Connected ◽

The One ◽

First Time

Background: Every year, men use cyclophosphamide to treat various cancers and autoimmune diseases. On the one hand, this chemotherapy often has the beneficial effect of regressing the tumor, but on the other hand, it leads to infertility due to excessive oxidative stress and apoptosis in the testes caused by its metabolite, acrolein. Methods: The objective of this study was to evaluate the beneficial power of a new compound called Hidrox®, containing 40–50% hydroxytyrosol, in counteracting the damage related to fertility induced by cyclophosphamide. The study was conducted using a single intraperitoneal injection of cyclophosphamide at a dose of 200 mg/kg b.w, in distilled water at 10 mL/kg b.w. The treatment was administered via the oral administration of Hidrox® at a dose of 50 mg/kg. Results: Our study confirms that the use of cyclophosphamide causes a series of sperm and histological alterations strongly connected with oxidative stress, lipid peroxidation, and apoptosis. Conclusion: Our results demonstrate for the first time that Hidrox® protects testes from CYP-induced alterations by the modulation of physiological antioxidant defenses.

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Reduction of oxidative stress in adjuvant arthritis. Comparison of efficacy of two pyridoindoles: stobadine dipalmitate and SMe1.2HCl.

Acta Biochimica Polonica ◽

10.18388/abp.2010_2398 ◽

2010 ◽

Vol 57 (2) ◽

Cited By ~ 7

Author(s):

Silvester Ponist ◽

Danica Mihalova ◽

Viera Jancinova ◽

Vladimír Snirc ◽

Olga Ondrejickova ◽

...

Keyword(s):

Oxidative Stress ◽

Adjuvant Arthritis ◽

Biochemical Parameters ◽

Therapeutic Potential ◽

Clinical Manifestations ◽

Thiobarbituric Acid ◽

Protein Adducts ◽

Gamma Glutamyltransferase ◽

Daily Dose ◽

First Time

The aim of this study was to evaluate the therapeutic potential of oxidative stress (OS) reduction by using pyridoindole (PI) antioxidants in adjuvant arthritis (AA). The substances tested were stobadine dipalmitate (STB) and SMe1. AA was used as animal model. The experiments included healthy animals, control arthritic animals and arthritic animals with administration of PI in the oral daily dose of 15 mg/kg b.m during 28 experimental days. The rats were sacrificed on day 28. Clinical and biochemical parameters were determined. The effect of PI administration was evaluated on the basis of the following parameters: (a) arthritis (volume of hind paws - HPW, change of animal body mass - CBM), (b) OS (chemiluminescence of whole blood - CWB, levels of thiobarbituric acid reacting substance - TBARS and of HNE- and MDA-protein adducts in plasma and activity of gamma-glutamyltransferase (GGT) in hind paw joint homogenates). The PI studied significantly increased the CBM of animals and corrected the HPW. STB also significantly decreased the activity of GGT in joint homogenates. SMe1 was more effective in decreasing plasmatic TBARS levels, but STB was more effective in reducing plasmatic HNE- and MDA-protein adducts. The assay for HNE- and MDA-adducts in plasma as a function of time was applied for the first time in AA. STB markedly decreased spontaneous and PMA-stimulated CWB and reduced neutrophil count. In summary, STB was more effective than SMe1 in reducing OS in AA. Our results showed that the reduction of OS in arthritis also corrected the clinical manifestations of the disease.

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Secondary metabolites from the resins of Aloe vera and Commiphora mukul mitigate lipid peroxidation

Acta Pharmaceutica ◽

10.2478/acph-2019-0027 ◽

2019 ◽

Vol 69 (3) ◽

pp. 433-441 ◽

Cited By ~ 4

Author(s):

Najeeb Ur Rehman ◽

Samia Ahmed Al-Riyami ◽

Hidayat Hussain ◽

Amjad Ali ◽

Abdul Latif Khan ◽

...

Keyword(s):

Oxidative Stress ◽

Lipid Peroxidation ◽

Chemical Constituents ◽

Aloe Vera ◽

Cellular Processes ◽

Structure Activity ◽

Anthraquinone Glycosides ◽

Commiphora Mukul ◽

Major Chemical ◽

First Time

Abstract Oxidative stress is often considered detrimental for cellular processes and damaging for the lipid bi-layer. Counteracting such stresses with the aid of nature-based chemical constituents can be an ideal therapeutic approach. The current study aimed to investigate the chemical constituents of resins derived from the well-known Aloe vera and less known Commiphora mukul trees and their effect in mitigating the lipid peroxidation (LPO) process. The bio-guided isolation of bio-active fractions from both resins afforded 20 chemical constituents (17 from A. vera and 3 from C. mukul). These compounds belonged to anthraquinones, anthraquinone glycosides, quinones, coumarins, polypodane-type terpenoids and benzene derivatives. Major chemical constituents of the resins of A. vera and C. mukul were from the classes of quinones and terpenoids. Feroxidin (4, from A. vera) showed slightly higher inhibition (IC50 = 201.7 ± 0.9 µmol L−1) than myrrhanone C (18, from C. mukul: IC50 = 210.7 ± 0.0 µmol L−1) and methyl 3-(4-hydroxyphenyl) propionate from A. vera (13, IC50 = 232.9 ± 0.2 µmol L−1) compared to the other compounds. Structure-activity relationship showed that the existence of hydroxyl, methoxy and ether groups might play a major role in countering oxidative stress. To the best of our knowledge, anti-LPO activities of compounds 1–4, 14, 18 and 20 are reported for the first time. Such chemical constituents with high anti-lipid peroxidation activity could be helpful in synthesizing candidate drugs.

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Oxidative stress/lipid peroxidation and antioxidant enzymes in apparently healthy individuals involved in physical exercise

Asian Journal of Medical Sciences ◽

10.3126/ajms.v7i6.15245 ◽

2016 ◽

Vol 7 (6) ◽

pp. 16-19

Author(s):

Ikekpeazu Joy Ebele ◽

Ikekpeazu Adanna Jenniger ◽

Eke Christian Nnabugo ◽

Ogbu Innocent Sidney ◽

Onyekwelu Kenechukwu Chibuike ◽

...

Keyword(s):

Oxidative Stress ◽

Lipid Peroxidation ◽

Antioxidant Enzymes ◽

Physical Exercise ◽

The Body ◽

Exercise Session ◽

Healthy Individuals ◽

Before And After ◽

Total Antioxidant ◽

Apparently Healthy

Background: Normal metabolic functions generate highly reactive compounds called free radicals in the body. Exercise is accompanied by increased oxygen consumption and enhanced Reactive Oxygen Species (ROS)/free radical production in humans. These molecules react with cellular molecules such as proteins, lipids and carbohydrates, and denature them. As a result of this, vital cellular structures and functions are lost and ultimately resulting in various pathological conditions.Aims and Objective: The objective of this study was to determine the effect of physical exercise on lipid peroxidation and antioxidant enzymes in apparently healthy individuals. Materials and Methods: A total of 96 samples were collected from apparently healthy adults aged between 18 and 25 years before and after morning exercise session. The samples were analyzed for Total Antioxidant Activity (TAS), pro-oxidant biomarker- malondialdehyde (MDA) and antioxidant enzymes – catalase (CAT) and glutathione peroxidase (GPx) by spectrophotometic method. Results: The result shows that TAS after exercise was significantly higher (p<0.001) than the level before exercise. The MDA level of the subjects after exercise was equally statistically significantly (p<0.001) higher than the level before exercise. This study therefore shows that aerobic exercise increases pro-oxidant markers and possibly induces oxidative stress, the effect of which is countered by an increase in TAS, CAT and GPx.Conclusion: Oxidative stress following exercise therefore does exist, but may not deter the benefits of physical exercise.Asian Journal of Medical Sciences Vol.7(6) 2016 16-19

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Oxidative stress in neonates with hyperbilirubinemia before and after phototherapy: malondialdehyde and catalase activity

Paediatrica Indonesiana ◽

10.14238/pi58.6.2018.269-73 ◽

2018 ◽

Vol 58 (6) ◽

pp. 269-73

Author(s):

Putu Junara Putra ◽

Rinawati Rohsiswatmo ◽

Pustika Amalia Wahidiyat

Keyword(s):

Oxidative Stress ◽

Lipid Peroxidation ◽

Free Radicals ◽

Catalase Activity ◽

Total Bilirubin ◽

Negative Association ◽

Control Group ◽

Induce Lipid Peroxidation ◽

Before And After ◽

Paired T Test

Background Phototherapy is used to treat neonatal hyperbilirubinemia, but is currently thought to cause photodynamic stress and can induce lipid peroxidation. There is increasing evidence that many severe diseases of the neonates are caused by oxidative injury and lipid peroxidation. In the present communique, we review the oxidative succeptibility of the neonate and the evidence now available that phototherapy induces oxidative stress. Malondialdehyde (MDA) is a metabolic product of free radicals. Catalase is a antioxidant that binds free radicals. Objective To compare the levels of oxidants and antioxidants before and after phototherapy in neonates with hyperbilirubinemia. Methods This pretest-posttest control group study was conducted in Sanglah Hospital, Bali from November 2016 to April 2017. Thirty babies with gestational age ≥35 weeks and hyperbilirubinemia with total bilirubin levels requiring phototherapy were included in this study. The MDA levels and catalase activity were measured before and after 24 hours of phototherapy. Results Comparative analysis using paired T-test showed a significant increase of malondialdehyde level, with mean MDA 23.73 (SD 8.20) nmol/mL before and 53.05 (SD 10.18) nmol/mL after phototherapy (P<0.001). However, catalase activity significantly decreased from of 72.33 (SD 10.63) kU/L before phototherapy to 44.85 (SD 14.79) kU/L after phototherapy (P<0.001). The MDA level had a significant, negative association with catalase activity after phototherapy (r =-0.4; P=0.028). Conclusion Neonates with hyperbilirubinemia are found to have increased oxidative stress after phototherapy, as indicated by increased MDA levels and decreased CAT activity after 24 hours of phototherapy.

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