scholarly journals Actions of Probiotics on Trinitrobenzenesulfonic Acid-Induced Colitis in Rats

2015 ◽  
Vol 2015 ◽  
pp. 1-8 ◽  
Author(s):  
Takahiko Shiina ◽  
Takeshi Shima ◽  
Kiyotada Naitou ◽  
Hiroyuki Nakamori ◽  
Yuuki Sano ◽  
...  

We investigated the actions of probiotics,Streptococcus faecalis129 BIO 3B (SF3B), in a trinitrobenzenesulfonic acid- (TNBS-) induced colitis model in rats. After TNBS was administered into the colons of rats for induction of colitis, the rats were divided into two groups: one group was given a control diet and the other group was given a diet containing SF3B for 14 days. There were no apparent differences in body weight, diarrhea period, macroscopic colitis score, and colonic weight/length ratio between the control group and SF3B group, suggesting that induction of colitis was not prevented by SF3B. Next, we investigated whether SF3B-containing diet intake affects the restoration of enteric neurotransmissions being damaged during induction of colitis by TNBS using isolated colonic preparations. Recovery of the nitrergic component was greater in the SF3B group than in the control group. A compensatory appearance of nontachykininergic and noncholinergic excitatory components was less in the SF3B group than in the control group. In conclusion, the present study suggests that SF3B-containing diet intake can partially prevent disruptions of enteric neurotransmissions induced after onset of TNBS-induced colitis, suggesting that SF3B has therapeutic potential.

Author(s):  
Waseem Muhammad Zia ◽  
Anjum Khalique ◽  
Saima Naveed ◽  
Jibran Hussain ◽  
Imran Muhammad ◽  
...  

A study to investigate the influence of selenium on final body weight and slaughter traits of Aseel was conducted. Three-weeks-old 120 cockerels from Lakha, Mushki, Peshaweri and Mianwali varieties of Aseel were randomly selected and distributed into four groups, 30 cockerels each, subdivided into 3 treatment groups A, B (experimental groups), and C (control group) with 10 cockerels in each treatment. Birds were assigned three experimental diets. The diet for the birds of group A incorporated 0.3 mg/kg Se-enriched yeast and group B included 0.3 mg/kg sodium selenite, while C was fed the diet without Se (control diet). Final body weight, dressed weight, dressing%, eviscerated weight, eviscerated%; chest, thigh and giblets weight; selenium content in chest and thigh in Se-yeast fed birds were significantly increased than those got sodium selenite or control diet. The study concluded that Se-enriched yeast is more effective in improving body weight and slaughtering characteristics of Aseel.


2007 ◽  
Vol 13 (2) ◽  
pp. 159-163 ◽  
Author(s):  
S. Navas-Carretero ◽  
A.M. Pérez-Granados ◽  
B. Sarriá ◽  
S. Schoppen ◽  
M.P. Vaquero

Fortifying food with iron has been widely studied as a strategy to prevent iron deficiency anaemia. This work comparatively assessed the bioavailability of two forms of iron, ferrous gluconate or ferric pyrophosphate encapsulated in liposomes (lipofer®), when used as fortificants in meat pate. Three groups of growing rats consumed during 28 days either a control diet (AIN-93G), or two diets prepared with enriched pate as the unique source of iron and fat. Body weight and diet intake were measured weekly, and during the last week faeces were collected. On day 28 animals were sacrificed, livers and spleens were removed and stored. Haemoglobin and total iron binding capacity (TIBC) were determined. There were not significant differences among the three groups in body weight and apparent iron absorption, although food intake in the two pate groups was significantly higher compared to the control group. There were not differences in liver and spleen iron content and concentration, neither in haemoglobin and TIBC values. These results indicated that iron bioavailability of pate enriched with ferrous gluconate or ferric pyrophosphate encapsulated in liposomes was similar, and thus both sources of iron are good candidates to be used as fortifiers in meat based products. The present outcome needs to be validated in humans.


2021 ◽  
pp. 096032712110134
Author(s):  
O Zouaoui ◽  
K Adouni ◽  
A Jelled ◽  
A Thouri ◽  
A Ben Chrifa ◽  
...  

Phytochemical composition and antioxidant activity of flowers decoction at post-flowering stage (F3D) of Opuntia dejecta were determined. The obtained findings demonstrate that F3D has a marked antioxidant activity in all tested assays. Furthermore, the present study was designed to test the protective activity of F3D against induced Diabetes type 2 (DT2) in male rats. Those metabolic syndromes were induced by a high-fructose diet (HFD) (10% fructose solution) for a period of 20 weeks. F3D was administered orally (100 and 300 mg/kg body weight) daily for the last 4 weeks. Metformin (150 mg/kg body weight) was used as a standard drug and administrated orally for the last 4 weeks. The results showed a significant increase in blood glucose, triglycerides and hepatic markers (ALAT, ASAT and ALK-P) in HFD group. A significant increase in hepatic TBARS and a significant decrease in SOD, CAT and GPX were observed in fructose fed rats compared to control group. Administration of F3D showed a protective effect in biochemical and oxidative stress parameters measured in this study. Also, oral administration of F3D restored the histological architecture of rat liver in comparison with rats fed HFD. In conclusion, F3D attenuated hepatic oxidative stress in fructose-fed rats.


2021 ◽  
Vol 11 (12) ◽  
pp. 5427
Author(s):  
Arturo Sanchez-Perez ◽  
Nuria Cano-Millá ◽  
María José Moya Villaescusa ◽  
José María Montoya Carralero ◽  
Carlos Navarro Cuellar

Photoactivation with ultraviolet C light can reverse the effects derived from biological ageing by restoring a hydrophilic surface. Ten titanium discs were randomly divided into three groups: a control group, a 6 W group, and an 85 W group. A drop of double-distilled, deionized, and sterile 10 µL water was applied to each of the discs. Each disc was immediately photographed in a standardized and perpendicular manner. Measurements were taken based on the irradiation time (15, 30, 60, and 120 min). UVC irradiation improved the control values in both groups. There was no difference in its effect between the 6 W group and the other groups during the first 30 min. However, after 60 min and up to 120 min, 85 W had a significantly stronger effect. The contact angles with the 85 W ultraviolet light source at 60 and 120 min were 19.43° and 31.41°, respectively, whereas the contact angles for the 6 W UVC source were 73.8° and 61.45°. Power proved to be the most important factor, and the best hydrophilicity result was obtained with a power of 85 W for 60 min at a wavelength of 254 nm.


2016 ◽  
Vol 5 (1) ◽  
pp. 26-34 ◽  
Author(s):  
M Aktaruzzaman ◽  
MS Islam ◽  
MMI Hasan ◽  
MJU Bhuiyan ◽  
MM Hossain ◽  
...  

This study aimed to evaluate the efficacy of ivermectin, levamisole HCl and albendazole against gastrointestinal nematodes in naturally infected goats of government goat development farm, Sylhet, Bangladesh. The study included 52 black Bengal breed of which 30 were naturally infected and randomly selected 20 on the basis of their weight and egg count. Twenty black Bengal goats of 12-13 month old irrespective of sex infested with gastrointestinal nematodes were selected for this experiment and randomly divided into four equal groups (group A, B, C and D) where each group consisted of 5 goats and goats of group D were kept as control group. One injectable ivermectin (200?gkg-1 body weight, S/C) preparations (Techno Drugs Ltd. Bangladesh) and two solid levamisole HCl, albendazole (7.5 mgkg-1 body weight, orally) preparations (The ACME Laboratories Ltd. and Square Pharmaceuticals Ltd. Bangladesh) were used for positive control of gastrointestinal nematodes as group A, B and C. Goats of group D was kept as control without giving any treatment. Before trials (day 0), total egg count, blood samples and initial body weight were recorded. During the study period the fecal and blood samples were collected directly from rectum and examined on 7th, 14th, 21st and 28th day using McMaster fecal egg counting method. Body weight was recorded on day 28 following the treatments. The results of the comparative efficacies of different anthelmintic of ivermectin was 86.75%, followed by levamisole HCl 85.07% and albendazole 92.81%. McMaster fecal egg counting method discloses the percentage of Haemonchus spp. (15.38%), with Trichostrongylus spp, Strongyloides spp., and Cooperia spp. also present. After treatment with ivermectin, levamisole HCl and albendazole, Total Erythrocyte Count (TEC), Hemoglobin (Hb) content and Packed Cell Volume (PCV) were increased significantly (p<0.01 and p>0.05) in goats but Erythrocyte Sedimentation Rate (ESR) and Total Leukocyte Count (TLC) were decreased significantly (p<0.05 and p>0.01) in all treated goat and body weight was increased significantly (p<0.01) on day 28.International Journal of Natural Sciences (2015), 5(1) 26-34


2021 ◽  
Vol 10 (2) ◽  
pp. 024-030
Author(s):  
Godwin Chinedu Uloneme ◽  
Demian Nnabuihe Ezejindu ◽  
Darlington Cyprian Akukwu ◽  
Amadi Chibundu Chiekezie

Background: The extract of Corchorus olitorius has a reasonable content of vitamins A and C, calcium, iron and fibre, and therefore enjoys a universal application in the treatment of some disease conditions, even as the whole leaf is a very important component of food in so many cultures. Purpose: The study was designed to investigate the effect of Corchorus olitorius extracts on the testis of adult Wistar rats. Method: A total number of thirty two adult Wistar rats weighing between 180 and 200 grammes separated into four groups labeled A,B,C and D respectively were used for the study. Animals in group A which served as the control group were fed with the normal rat chow and water only. The group B rats were administered 100mg/kg body weight of aqueous extract of Corchorus olitorus; while those in group C were administered 500mg/kg body weight of the extract. The group D rats received 1000mg/kg body weight of the extract. For a period of four weeks, the different experimental animal groups received the respective aforementioned treatments once daily, around nine- o’clock in the morning through oral intubation. At the end of the 28 day treatment, the animals were sacrificed and the testes harvested for histological, investigation, and through cardiac puncture, blood samples for some hormonal studies was also collected and investigated using standard laboratory standards. Results: Observations made showed that the extract produced no histological distortions, degenerative or defective effects on the testicular tissues. The testosterone levels of group B, C, and D rats were observed to be significantly higher (P<0.005) than that of the group A (control group).ound: The extract of Corchorus olitorius has a reasonable content of vitamins A and C, calcium, iron and fibre, and therefore enjoys a universal application in the treatment of some disease conditions, even as the whole leaf is a very important component of food in so many cultures. Purpose: The study was designed to investigate the effect of Corchorus olitorius extracts on the testis of adult Wistar rats. Method: A total number of thirty two adult Wistar rats weighing between 180 and 200 grammes separated into four groups labeled A,B,C and D respectively were used for the study. Animals in group A which served as the control group were fed with the normal rat chow and water only. The group B rats were administered 100mg/kg body weight of aqueous extract of Corchorus olitorus; while those in group C were administered 500mg/kg body weight of the extract. The group D rats received 1000mg/kg body weight of the extract. For a period of four weeks, the different experimental animal groups received the respective aforementioned treatments once daily, around nine- o’clock in the morning through oral intubation. At the end of the 28 day treatment, the animals were sacrificed and the testes harvested for histological, investigation, and through cardiac puncture, blood samples for some hormonal studies was also collected and investigated using standard laboratory standards. Results: Observations made showed that the extract produced no histological distortions, degenerative or defective effects on the testicular tissues. The testosterone levels of group B, C, and D rats were observed to be significantly higher (P<0.005) than that of the group A (control group).


2021 ◽  
Vol 42 (4) ◽  
pp. 2471-2488
Author(s):  
Giovanna Bobato Pontarolo ◽  
◽  
Mikael Neumann ◽  
Fernando Braga Cristo ◽  
Edelmir Silvio Stadler Júnior ◽  
...  

Functional additives, such as autolyzed yeasts, have been used to achieve greater production efficiency and animal health. These compounds are also alternatives to the use of performance-enhancing antimicrobials. The objective was to evaluate the productive performance, ingestive behavior, apparent digestibility of feed DM and the carcass characteristics of beef steers finished in feedlot receiving autolyzed yeasts in the diet. The experimental design was randomized blocks, consisting of three treatments and six repetitions, where each repetition was represented by a stall with two animals. 36 bulls, ½ Angus × ½ Nelore blood, from the same herd, with an average age of 11 months and an average body weight of ± 330 kg were used.The treatments were as follows: CON - yeast-free diet; Y4 - yeast diet (4 g animal day-1) and Y7 - yeast diet (7 g animal day-1). The product used is a functional ingredient containing the yeast Saccharomyces cerevisiae. The inclusion of autolyzed yeasts resulted in greater daily body weight gain and better feed conversion in the initial feedlot phase. The apparent digestibility of DM of diets containing autolyzed yeast was superior to the control diet, and its use did not interfere with the animal ingestive behavior. Supplementation with autolyzed yeasts at the inclusion level of 4 g day-1 promoted better results in the finishing of feedlot steers, and that the supplemented groups (4 g animal day-1 and 7 g animal day-1) achieved the same degree of carcass finishing, higher than the control group.


2000 ◽  
Vol 83 (5) ◽  
pp. 561-568 ◽  
Author(s):  
C. Feillet-Coudray ◽  
C. Coudray ◽  
D. Bayle ◽  
E. Rock ◽  
Y. Rayssiguier ◽  
...  

There is a lack of agreement on index of Cu status and reliable and sensitive biomarkers are still required. The purpose of this present work was to assess in rats the sensitivity of diamine oxidase (DAO) activity, a recently proposed biomarker, to modifications in dietary Cu intake in comparison with other plasma biomarkers of Cu status. We also evaluated the effect of Cu dietary level on Cu and Zn intestinal absorption. Results showed that plasma Cu and plasma caeruloplasmin were significantly decreased at day 8 compared with the control group (7·4 mg Cu/kg diet) while DAO activity was significantly decreased at day 12 of the deficient diet (0·61 mg Cu/kg diet). Cu supplementation (35 mg Cu/kg diet) had no effect on any of the studied biomarkers of Cu status. In Cu-deficient rats plasma Cu and DAO activities were normalized 4 d after return to the control diet while caeruloplasmin was normalized later, at day 11. Apparent absorption values (%) of total Cu or65Cu isotope were significantly increased in the Cu-deficient rats compared with the other groups and similar in the control and the Cu-supplemented groups. The urinary excretion of total Cu or65Cu isotope were increased in the Cu-supplemented group compared with the other two groups. Both apparent absorption and urinary excretion of total Zn or67Zn isotope remained unchanged in the three experimental groups. In conclusion, DAO activity seemed to be less sensitive to Cu deficiency than plasma Cu or caeruloplasmin concentrations. The present study also showed a significant increase in Cu intestinal absorption with dietary Cu restriction but no decrease with Cu supplementation in the rat.


2014 ◽  
Vol 11 (1) ◽  
pp. 1-5 ◽  
Author(s):  
S Khatun ◽  
M Mostofa ◽  
F Alom ◽  
J Uddin ◽  
MN Alam ◽  
...  

The efficacy of tulsi (Ocimum sactum) and neem (Azadirachta indica) leaves extract as a growth promoter were studied in broiler. A total of 40 day-old broiler chicks were purchased from Kazi hatchery and after three days of acclimatization the chicks were randomly divided into four groups (n=10). No vaccination schedule was practiced and no antibiotic was added in ration of group A, B, C, and D respectively. Group A served control without any supplements while group B, C and D were supplemented with combination of tulsi and neem extract @ 1 ml, 2ml and 3 ml/liter of drinking water. Live body weight gain was recorded weekly up to 6th weeks and hematological studies were performed at 21st and 42nd day of experiments. At the end of 42nd day of experiment final body weight of group A, B, C and D were 1561± 12.10 g, 1698± 12.87 g, 1608± 12.04 g and 1763± 13.28 g, respectively. The birds of group D utilized their feed more efficiently among the treatment groups (p<0.05). The net body weight gain were, 1533± 11.98 g, 1443± 11.32 g and 1588± 12.10 g in treated groups compared to control group (1393± 11.07 g ) and total net profit per broiler was 19.08, 40.10, 20.68 and 45.07 Taka , respectively. Hematological parameterss (TEC, PCV, Hb and ESR) were not significantly changed among the treated and control group suggesting no side effects of herbal extracts in broiler. It can be concluded that tulsi and neem extract is economic and safe in broiler production.DOI: http://dx.doi.org/10.3329/bjvm.v11i1.17728 Bangl. J. Vet. Med. (2013). 11 (1): 1- 5


2016 ◽  
Vol 14 (1) ◽  
pp. 37-42 ◽  
Author(s):  
M. G. Sorwar ◽  
M. Mostofa ◽  
M. N. Hasan ◽  
M. Billah ◽  
M. T. Rahman

This experiment was conducted to determine the effect of papaya leaf (Carica papaya) and kalo jeera (Nigella sativa) seeds powdered supplementation in drinking water as a growth promoter in broiler chickens. A total of 20 Cobb-500 broiler chicks (day-old) were purchased from local hatchery (Nourish Poultry and Hatchery Ltd.) and after seven days of acclimatization chicks were randomly divided into two groups, A (n=10) and B (n=10). The group A was kept as a control and not treated. The group B was supplemented with papaya leaf and kalo jeera powder with feed and water. Weekly observations were recorded for live body weight gain up to 5th weeks and hematological tests were performed at 35th day’s age of broiler to search for hematological changes between control (A) and treatment (B) groups. The initial body weight of groups A and B on 1st were 41.00±0.56 gm and 41.50±0.35 gm, respectively and after 35th day of experiment final body weight were 1470±57.35 gm and 1720±58.56 gm, respectively and economics of production were analyzed and found that net profit per broiler was Tk. 8.91 and Tk. 20.69, respectively. The treatment group B was recorded statistically significant (at 1% level) increased (17.00%) for live body weight than that of control group A. The hematological parameters total erythrocyte count (TEC), erythrocyte sedimentation rate (ESR) and hemoglobin (Hb) estimation value of treatment group shows significant difference, while hemoglobin estimation does not show significant difference from control group. The results suggest that better growth performance could be achieved in broilers supplemented with papaya leaf and kalo jeera seeds.


Sign in / Sign up

Export Citation Format

Share Document