Fungal Biomass Protein Production fromTrichoderma harzianumUsing Rice Polishing

Industrially important enzymes and microbial biomass proteins have been produced from fungi for more than 50 years. High levels of crude protein as much as 45% are present in fungal biomass with balanced essential amino acids. The aim of this study was to access the potential ofTrichoderma harzianumto produce fungal biomass protein from rice polishings. Maximum biomass yield was obtained at 5% (w/v) rice polishings after 72 h of incubation at 28°C at pH 4. Carbon and nitrogen ratio of 20 : 1 gave significantly higher production of fungal biomass protein. The FBP in the 75 L fermenter contained 49.50% crude protein, 32.00% true protein, 19.45% crude fiber, 9.62% ash, 11.5% cellulose content, and 0.325% RNA content. The profile of amino acids of final FBP exhibited that all essential amino acids were present in great quantities. The FBP produced by this fungus has been shown to be of good nutritional value for supplementation to poultry. The results presented in this study have practical implications in that the fungusT. harzianumcould be used successfully to produce fungal biomass protein using rice polishings.

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The nutritional value, bacterial count and sensory attributes of little tuna (Euthynnus affinis) floss incorporated with the banana blossom

Potravinarstvo Slovak Journal of Food Sciences ◽

10.5219/1657 ◽

2021 ◽

Vol 15 ◽

pp. 846-857

Author(s):

Hartati Kartikaningsih ◽

Yahya Yahya ◽

Trihartita Yuniar ◽

Abdul Aziz Jaziri ◽

Wahidu Zzaman ◽

...

Keyword(s):

Amino Acids ◽

Nutritional Quality ◽

Crude Protein ◽

Nutritional Value ◽

Bacterial Count ◽

Essential Amino Acids ◽

National Standard ◽

Crude Protein Content ◽

Crude Fibre ◽

Euthynnus Affinis

The study aimed to evaluate the addition of banana blossom (12.5, 25, 37.5, and 50% w/w) on nutritional quality, histamine content, bacterial count, and sensory characteristic in the fish floss prepared from little tuna (Euthynnus affinis). The crude protein content, essential amino acids, lipid, and polyunsaturated fatty acids (PUFA) steadily decreased (p <0.05), while the crude fibre, carbohydrate, and ash components of the tuna floss, increased significantly (p <0.05) with increasing levels of banana blossom. The contents of protein, fat, ash, fibre, carbohydrate, and moisture ranged 28.13 – 30.27%, 14.79 – 18.02%, 4.45 – 5.68%, 2.6 – 3.5%, 27.81 – 31.01, and 16.45 – 17.39%, respectively, and most of them met the Indonesian National Standard. For essential and non-essential amino acids, the level varied about 102.82 mg.g-1 to 206.76 mg.g-1 and 79.71 mg.g-1 to 138.76 mg.g-1, respectively in the treated tuna flosses. Moreover, ranging 13.72 – 16.29% of PUFA was found in all treated flosses. The most significant effect was found in the histamine levels of the tuna flosses, especially in the 50% added floss sample. Moreover, bacterial counts and heavy metals content were lower than the maximum limits regulated by the Indonesian National Standard. For sensory evaluation, the banana blossom-added samples significantly increased (p >0.05) the acceptability score for all attributes assessed. Taken together, the tuna floss added with 37.5% of banana blossom may be potentially developed as a low-histamine tuna-based product with high ffibre andEPA+DHA, as well as highly acceptable for consumers.

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Nutritive value of mustard cake derived from seeds of different genetic make up for growing chicks

The Journal of Agricultural Science ◽

10.1017/s0021859600059918 ◽

1978 ◽

Vol 91 (3) ◽

pp. 543-550 ◽

Cited By ~ 3

Author(s):

V. K. Cilly ◽

G. N. Lodhi ◽

J. S. Ichhponani

Keyword(s):

Amino Acids ◽

Crude Protein ◽

Nutritive Value ◽

Energy Content ◽

Amino Acid Content ◽

Essential Amino Acids ◽

Metabolizable Energy ◽

Crude Fibre ◽

Groundnut Cake ◽

True Protein

SummaryCakes derived from Taramira (Eruca sativa), Raya (Brassica juncea), Toria (B. campestris var. toria) and yellow and brown sarson (B. campestris var. sarson) were evaluated for their contents of crude protein, true protein, essential amino acids, available carbohydrate and tannins. Feeding trials were also conducted to compare the suitability of these cakes with groundnut cake for broilers and White Leghorn chicks up to 4 weeks of age.The crude protein content of Taramira cake was 33% whereas all the other cakes contained 37–38%. True protein accounted for 80–83% of crude protein in all the varieties and albumin and globulin constitutes the bulk of protein. The critical amino acid content of all the brassica seed proteins was found to be higher than that of other vegetable proteins although varietal differences were observed with respect to a few essential amino acids. The hulls accounted for 19% of whole Brassica seed and their removal raised the concentration of protein from 39 to 46% and reduced the crude fibre content from 15 to 6% in the defatted meal. Tannin content was higher in Taramira (1·74%) than in all other varieties (1·0–1·4%) and the major quantity of it was localized in the endosperm. The average metabolizable energy content of all the mustard cakes was 9·62 and 8·75 MJ/kg for meat-type and egg-type chicks, respectively. The nutritive value of Taramira cake was poorer than that of other Brassica cakes or groundnut cake for supporting growth rate of chicks of either breed.

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Comparison on Nutrient Composition and Amino Acid and Fatty Acid Profile of Muscle in Wild and Farmed Carassius auratus

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.647.288 ◽

2013 ◽

Vol 647 ◽

pp. 288-292

Author(s):

Xing Hong Xu ◽

Xiang Liu

Keyword(s):

Amino Acids ◽

Fatty Acids ◽

Amino Acid ◽

Carassius Auratus ◽

Crude Protein ◽

Nutritional Value ◽

Essential Amino Acids ◽

Fatty Acids Profile ◽

Department Of Health ◽

Wild Group

The nutritional compositions, including amino acids and fatty acids profile in muscle were compared between wild and farmed Carassius auratus to evaluate the nutritional value. The contents of moisture, crude protein, fat, carbohydrate and ash in wild C. auratus were 74.47%, 19.12%, 3.72%, 1.52% and 1.18％, and those of farmed C. auratus were 74.03%, 18.41%, 4.54%, 1.95% and 1.06％, respectively. The contents of total amino acids (TAA) and essential amino acids (EAA) in wild C. auratus were obviously higher than those of farmed group (dry sample), whereas the protein of farmed C. auratus has more well-balanced amino acid composition based on the Amino acid scores. Abundant polyunsaturated fatty acids (PUFAs) were observed in the muscles of two groups, however the ratio of n-6/n-3 especially the wild group were significantly higher than the value (4.0 at maximum) recommended by UK Department of Health and the value of China (4:1～6:1). So the fatty acids composition of C. auratus was not ideal for the human health.

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THE CHARACTERISTIC OF COMPOUND FEEDS FOR TILAPIA FISH

Grain Products and Mixed Fodder’s ◽

10.15673/gpmf.v19i2.1445 ◽

2019 ◽

Vol 19 (2) ◽

pp. 32-36 ◽

Cited By ~ 1

Author(s):

L. V. FIHURSKA ◽

V. V. THORENKO,

Keyword(s):

Amino Acids ◽

Crude Protein ◽

Nutritional Value ◽

Fish Farming ◽

Essential Amino Acids ◽

Crude Fiber ◽

Feed Consumption ◽

World Volume ◽

The World ◽

Gross Energy

The article states that, according to the literature review, in aquaculture of freshwater fish species that acquire everincreasing production volumes, tilapia is leading with 17%, and it is follower by carp (15% of world volume). For almost 60 years, tilapia have moved into second place in the global fish production, with the prospect of taking first place in the next 2-3 years and overtake carp in world production. Since fish is a rich source of proteins and essential amino acids, vitamins, polyunsaturated fatty acids, macro and micronutrients, it is also an important component of the diet of the population. Meat of tilapia is dense, non-fat, protein content close to trout meat, does not contain intermuscular bones. One of the priorities of the development of fish farming in Ukraine is the cultivation of tilapia. The amount of feed consumption in the world is increasing every year, due to the rapid growth in demand for tilapia. Currently, Ukrainian businessmen are interested in the production of catfish and tilapia. The most famous aquapride plants that grow these breeding objects are Lauren Aquaculture LLC (Rivne region), Aqua System Organic LLC (Kiev region), Catfish from Pavlysh TM (Kirovograd region), TM “First City Fish Farm ‘’I want a catfish ”(Kiev). That is why the production of balanced domestic feed for tilapia is an important task of the feed industry of Ukraine. The theoretical study was devoted to the issue of tilapia feed production. The nutritional value of feed for tilapia depends on the stage of the life cycle of this species of fish. It was shown the world leading manufacturers of feed for tilapia, it was analyzed the nutritional value of feed for this species of fish on the content of crude protein in the starting, growth and finishing periods, respectively. The requirements for the content of essential nutrients, essential amino acids, minerals (micro and micronutrients), water-and fat-soluble vitamins and restrictions on the content of crude fiber are given. The programs of feeding of tilapia of various producers are analyzed and an own program of feeding this species of fish is developed, it is divided the starting, growth and finishing period of tilapia growing. For starter mixed feeds for tilapia, the content of crude protein should be at least 45%, crude fat at least 12%, crude fiber not more than 1.5%, gross energy not less than 18 MJ, crude ash not more than 8%. For grower’s feeds, the content of crude protein should be at least 40 %, crude fat at least 12%, crude fiber not more than 3%, gross energy not less than 17 MJ, crude ash not more than 9.5%. For finishing feeds, the content of crude protein should be at least 30 %, crude fat at least 6%, crude fiber not more than 5%, gross energy not less than 17 MJ, crude ash not more than 7%. Amino acids requirements are very important for tilapia fish, feeds for tilapia from any period should contain lysine should be at least 1.6%, methionine should be at least 0.7 %.

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THE CHEMICAL COMPOSITION OF AQUATIC MACROPHYTES. II. AMINO ACID COMPOSITION OF THE PROTEIN AND NON-PROTEIN FRACTIONS

Canadian Journal of Plant Science ◽

10.4141/cjps78-123 ◽

1978 ◽

Vol 58 (3) ◽

pp. 843-849 ◽

Cited By ~ 14

Author(s):

A. JABBAR MUZTAR ◽

S. J. SLINGER ◽

J. H. BURTON

Keyword(s):

Amino Acids ◽

Amino Acid ◽

Crude Protein ◽

Essential Amino Acids ◽

Amino Acid Profile ◽

Reference Pattern ◽

Protein Nitrogen ◽

Leaf Protein Concentrate ◽

True Protein ◽

Method Of Extraction

The amino acid profile, crude protein (N × 6.25) and true protein (sum of all amino acids) levels were investigated in washed samples of several species of aquatic plants. There were differences of as much as 6% in the levels of crude protein and true protein of some of the species analyzed. Glutamic acid, aspartic acid and glycine, among the non-essential amino acids, and leucine, lysine, arginine and valine among the essential amino acids were major contributors to proteins in these plants. Levels of essential amino acids in the plants under investigation compared favorably with the FAO reference pattern and leaf protein concentrate. The non-protein nitrogen (NPN) and free amino acids (FAA) remaining in solution following the precipitation of protein using 80% ethanol, 5% TCA or 10% TCA were also determined in four species. Less total NPN remained in solution following ethanol extraction than when either 5 or 10% TCA was used. In addition to amino acids normally occurring in protein, ornithine, citrulline, asparagine, glutamine and -γ-amino butyric acid were also present in the NPN fraction. The FAA content ranged from approximately 6.6 to 16.8% of the total NPN, depending upon species of plant and method of extraction. The analysis indicated that this fraction could be nutritionally utilized by monogastric animals.

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Screening of amino acid constituents from date palm fruits

International Journal of Bioassays ◽

10.21746/ijbio.2016.10.0011 ◽

2016 ◽

Vol 5 (10) ◽

pp. 4972

Author(s):

Lata Birlangi

Keyword(s):

Amino Acids ◽

Amino Acid ◽

United Arab Emirates ◽

Nutritional Value ◽

Date Palm ◽

Amino Acid Content ◽

Essential Amino Acids ◽

Cultivated Plants ◽

Palm Fruits ◽

Social Part

The date palm (Phoenix dactylifera L.) is one of mankind’s oldest cultivated plants. The fruit of the date palm is an important crop of the hot arid and semi-arid regions of the world. It has always played a genuine economic and social part in the lives of the people of these areas. The present objective in examining the amino acid content of different varieties of date palm fruits from Middle-East region; is to determine whether its protein could effectively supplement the nutritional value and it is also aimed in finding which variety is rich in number of amino acids. The phytochemical screening revealed the presence of eight essential amino acids and five non-essential amino acids in the date fruits. Among all the date fruit varieties taken as samples for the study, Dabbas cultivar of United Arab Emirates found to exhibit eight types of amino acids which includes five as non-essential ones. Total of thirteen amino acids were detected in the seven date cultivars. Determination of amino acid can serve as a guide to the possible nutritional value.

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Progress towards reduced-crude protein diets for broiler chickens and sustainable chicken-meat production

Journal of Animal Science and Biotechnology ◽

10.1186/s40104-021-00550-w ◽

2021 ◽

Vol 12 (1) ◽

Author(s):

Sonia Yun Liu ◽

Shemil P. Macelline ◽

Peter V. Chrystal ◽

Peter H. Selle

Keyword(s):

Amino Acids ◽

Amino Acid ◽

Broiler Chickens ◽

Crude Protein ◽

Plasma Concentrations ◽

Essential Amino Acids ◽

Chicken Meat ◽

Meat Production ◽

Accurate Identification ◽

Reduced Crude Protein

AbstractThe prime purpose of this review is to explore the pathways whereby progress towards reduced-crude protein (CP) diets and sustainable chicken-meat production may be best achieved. Reduced-CP broiler diets have the potential to attenuate environmental pollution from nitrogen and ammonia emissions; moreover, they have the capacity to diminish the global chicken-meat industry’s dependence on soybean meal to tangible extents. The variable impacts of reduced-CP broiler diets on apparent amino acid digestibility coefficients are addressed. The more accurate identification of amino acid requirements for broiler chickens offered reduced-CP diets is essential as this would diminish amino acid imbalances and the deamination of surplus amino acids. Deamination of amino acids increases the synthesis and excretion of uric acid for which there is a requirement for glycine, this emphasises the value of so-called “non-essential” amino acids. Starch digestive dynamics and their possible impact of glucose on pancreatic secretions of insulin are discussed, although the functions of insulin in avian species require clarification. Maize is probably a superior feed grain to wheat as the basis of reduced-CP diets; if so, the identification of the underlying reasons for this difference should be instructive. Moderating increases in starch concentrations and condensing dietary starch:protein ratios in reduced-CP diets may prove to be advantageous as expanding ratios appear to be aligned to inferior broiler performance. Threonine is specifically examined because elevated free threonine plasma concentrations in birds offered reduced-CP diets may be indicative of compromised performance. If progress in these directions can be realised, then the prospects of reduced-CP diets contributing to sustainable chicken-meat production are promising.

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Herring silage as a protein supplement for young cattle

Canadian Journal of Animal Science ◽

10.4141/cjas91-140 ◽

1991 ◽

Vol 71 (4) ◽

pp. 1187-1196 ◽

Cited By ~ 1

Author(s):

J. W. G. Nicholson ◽

D. A. Johnson

Keyword(s):

Amino Acids ◽

Free Amino ◽

Crude Protein ◽

Fish Meal ◽

Unsaturated Fatty Acids ◽

Rumen Fluid ◽

Potassium Sorbate ◽

Protein Supplement ◽

Young Cattle ◽

True Protein

Fish silage made by grinding herring and adding formic acid, β-hydroxytoluene and potassium sorbate was evaluated as a protein supplement for young cattle. Only about 15% of the crude protein in the herring silage was true protein. Ammonia N accounted for 8% of the crude protein and most of the rest was peptides and free amino acids. The crude protein of herring silage was as resistant as fish meal to deamination when fermented in rumen fluid, and more resistant than soybean or casein. The herring silage was readily accepted by Holstein heifers fed hay or grass–legume silage with potatoes (7 kg d−1) and a supplement (1.5 kg d−1). Feed intake and weight gain were similar when the heifers were fed hay with either soybean meal or herring silage but were higher when forage silage replaced the hay. Rumen fluid NH3-N and blood urea levels were normal, even for cattle fed the high non-protein N diet of forage silage with herring silage. The herring silage depressed rumen fluid acetate levels and increased propionate in the heifers fed hay + potatoes, probably because of the unsaturated fatty acids in the herring. Well-made herring silage was a suitable protein supplement for young cattle fed forage and potato diets. Key words: Herring silage, fish silage, potatoes, cattle, protein degradation

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Composition and Nutritional Evaluation of the Cuttlefish Muscle

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.554-556.1553 ◽

2012 ◽

Vol 554-556 ◽

pp. 1553-1558

Author(s):

Sheng Jun Chen ◽

Lai Hao Li ◽

Xian Qing Yang ◽

Bo Qi ◽

Yan Yan Wu ◽

...

Keyword(s):

Amino Acids ◽

Fatty Acids ◽

Crude Protein ◽

Nutritive Value ◽

Saturated Fatty Acids ◽

Monounsaturated Fatty Acids ◽

Essential Amino Acids ◽

Nutritive Quality ◽

High Quality Protein ◽

Acid Index

The nutritional components in the cuttlefish muscle were analyzed and the nutritive quality was evaluated in the paper. The results showed that the contents of the cuttlefish muscle in crude protein, crude fat, carbohydrate were 16.60%, 0.86%, 1.30%, respectively. There were 18 kinds amino acids and the total amino acids reached 17.44%, with an essential amino acid index(EAAI) of 67.95. The composition of the essential amino acids in muscle ratio was consistent with the FAO/WHO standards. In addition, the contents of saturated fatty acids, monounsaturated fatty acids and polyunsaturated fatty acids were 41.13%, 12.15% and 46.72%, respectively. And the muscle contains a higher content of EPA and DHA, reached 11.0% and 24.49%, respectively. It was considered that the cuttlefish muscle has a high nutritive value and it can be the important material of the high quality protein and unsaturated fatty acid.

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