scholarly journals Enzyme Producing Activity of Probiotics and Preparation of Compound Enzyme

2020 ◽  
Vol 2020 ◽  
pp. 1-8
Author(s):  
Ruokun Yi ◽  
Yanni Pan ◽  
Xingyao Long ◽  
Fang Tan ◽  
Xin Zhao
Keyword(s):  
Intestinal Flora ◽  
Control Group ◽  
Water Control ◽  
Enzyme Preparations ◽  
Preventive Drug ◽  
Set Up ◽  
Experimental Group ◽  
Final Effect ◽  
Single Factor Experiment ◽  
Compound Enzyme

Probiotics are a group of active microorganisms, which benefit the host by colonizing and changing the composition of host flora. It is of great significance to promote the development of human gastrointestinal nutrition and health by regulating the host mucosal and systemic immune function or regulating the balance of intestinal flora. The purpose of this study is to analyze the production activity of the enzyme, evaluate its biological characteristics and safety as a preventive drug, and provide reference for the research of enzyme production and compound enzyme preparation by probiotics. In this study, four groups of probiotics were set up: Clostridium butyricum experimental group, Lactobacillus plantarum experimental group, drinking water control group, and Bacillus licheniformis experimental group. In addition, a variety of complex enzyme experiments were set up to study the influence on the digestive tract and single factor experiment. The results showed that probiotics and compound enzyme preparations could significantly promote the intestinal digestibility. Under the effect of probiotics, the weight of the chicken was almost 1 Jin heavier than that of the control group, and the average digestibility was increased by 4.3%. The effect of the enzyme on digestibility is stronger than that of probiotics, but the final effect tends to be stable.

scholarly journals The economic efficiency of the use of mixed ligand complexes of Zinc, Manganese and Cobalt in the rations of highlyproductive cows of the Ukrain-ian Red-Spotted Dairy breed

2021 ◽  
Vol 23 (95) ◽  
pp. 82-86
Author(s):  
Yu. G. Kropyvka ◽  
V. S. Bomko ◽  
S. Y. Kropyvka
Keyword(s):  
Economic Efficiency ◽  
Economic Effect ◽  
Optimal Dose ◽  
Mixed Ligand ◽  
Mixed Ligand Complexes ◽  
Control Group ◽  
Feed Mixture ◽  
Set Up ◽  
Experimental Group ◽  
Dairy Breed

The article deals with the results of research on the economic efficiency of the use of mixed ligand complexes of Zinc, Manganese and Cobalt in the feeding rations of highlyproductive cows of Ukrainian Red-Spotted Dairy breed in the first 100 days of lactation. The research was performed on five groups (one control and four experimental) of analogous cows in the conditions of ALC “Terezyne” Bila Tserkva district of Kyiv region. The control was the optimal dose of mixed ligand complexes of Zinc, Manganese and Cobalt, which was previously established with a concentration of 1 kg of dry matter (DM) of the feed mixture (FM), mg: Zinc – 60.8; Manganese – 60.8 and Cobalt – 0.78. For the 2nd experimental group, the concentration of these trace elements was increased by 10 %, and in the 3rd, 4th and 5th experimental groups – on the contrary, the concentration of Zinc, Manganese and Cobalt in 1 kg of DM of feed mixture was reduced by 10 %, 20 and 30 %, respectively, compared with the control. The best results on milk yield of natural and basic fat content were got from cows of the 4th experimental group, where the concentration of Zinc, Manganese and Cobalt due to their mixed ligand complexes in 1 kg of DM of feed mixture was, mg: Zinc – 48.6; Manganese – 48.6; Cobalt – 0.62. The cost of products got from experimental cows ranged from UAH 12702.3 up to UAH 13783.7 and it was the highest in the 4th experimental group. It was set up that for cows of Ukrainian Red-Spotted Dairy breed in the first 100 days of lactation the most economically advantageous was the dose of mixed ligand complexes, in which the concentration of Zinc and Manganese was 48.6 mg, and Cobalt – 0.62 mg/kg DM, which allowed to obtain a profit of 14.37 % more than in the control group. Doses of mixed ligand complexes with a concentration of Zinc and Manganese – 66.9 mg; 54.7 and 42.6 mg, and Cobalt – 0.86 mg; 0.7 and 0.55 mg/kg CF gave a much lower economic effect. Additional profit in the 2nd experimental group amounted to UAH 66.8 or 1.34 %; in the 3rd – UAH 95.6 or 1.92 % and in the 5th – UAH 561.6 or 11.28 %, compared with the control group.

Relationship between Lactase Activity and Intestinal Flora Structure

2021 ◽  
Vol 7 (5) ◽  
pp. 3104-3109
Author(s):  
Ying Zhang ◽  
Mingjian Huang ◽  
Jianfeng Chen ◽  
Shuyuan Wang ◽  
Jian Xu
Keyword(s):  
Young Children ◽  
Intestinal Tract ◽  
Lactose Intolerance ◽  
Intestinal Flora ◽  
Control Group ◽  
Lactase Activity ◽  
Beneficial Bacteria ◽  
The Relationship ◽  
Experimental Group ◽  
Infants And Young Children

To analyze and explore the relationship between lactase activity and the structure of intestinal flora in infants under 1 year old. Method: Our hospital selected 60 infants and young children in the Haizhu Maternity and Child Health Hospital from August 2019 to September 2020 as the research objects. The infants in the experimental group were diagnosed as lactose intolerant infants, the control group were normal infants, and the experimental group and control group had 30 cases each. The relationship between lactase activity and intestinal flora structure. Result: The experimental group had fewer beneficial bacteria in the intestinal tract than the control group, but improved lactase activity by correcting the structure of the intestinal flora and alleviating symptoms of lactose intolerance. Conclusion: Infants and young children are prone to non-infectious diarrhea, and the causes are more complicated, but most of them are related to lactose intolerance. When infants and young children develop lactose intolerance, it is necessary to pay attention to the lack of beneficial bacteria in the intestinal tract. Targeted supplementation of probiotics (such as Lactobacillus reuteri, Clostridium butyricum, etc.) can correct the intestinal flora, improve lactase activity, and relieve symptoms of lactose intolerance.

scholarly journals Effect of Feed Supplemented With Vitamin C on Microbial Flora of Swine Faeces and Blood Morphology

2012 ◽  
Vol 56 (2) ◽  
pp. 171-175
Author(s):  
Beata Trawińska ◽  
Jerzy Lechowski ◽  
Antoni Polonis ◽  
Marta Kowaleczko
Keyword(s):  
Vitamin C ◽  
Pathogenic Bacteria ◽  
Bacterial Species ◽  
Intestinal Flora ◽  
Bacterial Count ◽  
Control Group ◽  
Total Bacterial Count ◽  
E Coli ◽  
Bacteriological Evaluation ◽  
Experimental Group

Abstract The studies involved gilts 6 weeks pre-partum allocated into the control and experimental groups. The experimental group received vitamin C at the dose of 2.5g/100 kg b.w./day. Faeces for analyses were collected for 3- and 6- week period of vitamin C dietary inclusion. The faecal material underwent quantitative and qualitative bacteriological evaluation. The count of Enterobacteriaceae was evaluated. The qualitative examination of these bacteria was made using the micro-plate method API 20E. Feed supplemented with vitamin C contributed to a statistically significant decline of the total bacterial count of Enterobacteriaceae in faeces. The qualitative bacterial analyses showed the presence of E. coli, Providecia sp., Proteus sp., and Salmonella sp. in the control and experimental groups. Enterobacter sp. was recovered only in the control group, while Lactobacillus sp. in the experimental group. The dietary administration of vitamin C significantly decreased the number of the studied bacterial species, except Salmonella rods. No statistically significant differences in the number of most blood morphotic elements following the 6- week supply of vitamin C were found; only the granulocyte count significantly increased, while lymphocyte numbers declined. Ascorbic acid inhibited the growth of pathogenic intestinal flora and reduced the pathogenic and relatively pathogenic bacteria count in the gastrointestinal tract and notably contributed to enhanced growth of beneficial bacteria.

scholarly journals Analysis of Intestinal Flora and Levels of Epidermal Growth Factor Receptor, Interleukin-32, and Gastrin 17 in Patients with Gastric Cancer via Carbon Nanoparticle Laparoscopy

2021 ◽  
Vol 2021 ◽  
pp. 1-8
Author(s):  
Liping Bai ◽  
Fubing Yu ◽  
Lixian Bai ◽  
Yinhui Zhang ◽  
Zhi Li ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Lymph Node ◽  
Intestinal Flora ◽  
Growth Factor Receptor ◽  
Serum Levels ◽  
Control Group ◽  
Carbon Nanoparticle ◽  
Laparoscopic Operation ◽  
Distal Gastric Cancer ◽  
Experimental Group

In order to explore the changes of intestinal flora and serum levels of relevant substances in patients with gastric cancer before and after surgery with carbon nanoparticle laparoscopy, a total of 180 patients with early distal gastric cancer who adopted laparoscopic radical gastrectomy for distal gastric cancer in the general surgery department of TCM Hospital of Shi Jia Zhuang City from January 2018 to January 2020 were selected and randomly divided into two groups: traditional laparoscopic operation (control group) and carbon nanoparticle laparoscopic operation (experimental group) were adopted for treatment for the two groups, respectively. Postoperative evaluation included the difference between the two groups in the operative time, the efficiency of intraoperative lymph node dissection, and the number of lymph node detection. The adverse reactions, changes of intestinal flora before and after surgery in the two groups, and the serum levels of epidermal growth factor receptor (EGFR), interleukin-32 (IL-32), and gastrin 17 were evaluated. In the experimental group, the success rate of carbon nanoparticle tracer black staining reached 100%, and the operation time of the experimental group was significantly shorter than that of the control group ( P < 0.05 ). The lymph node detection rate of the experimental group was higher than that of the control group ( P < 0.05 ), but there was no significant difference in the lymph node metastasis rate between the two groups ( P > 0.05 ). The sentinel lymph node sensitivity of the experimental group reached 92.3%, and the specificity, accuracy, and positive and negative prediction rates reached 100%; the experimental group patients were with an obviously higher incidence of level I-II gastrointestinal reaction ( P < 0.05 ). Postoperative increases in Bifidobacteria and Lactobacillus were observed in both groups, while decreases in Enterococcus and Escherichia coli were observed in both groups ( P < 0.05 ). Moreover, the degree of increase and decrease in the experimental group was greater than that in the control group ( P < 0.05 ). The serum levels of EGFR, IL-32, and gastrin 17 in the two groups were significantly lower than those in the control group on 3 d, 7 d, and 15 d after surgery ( P < 0.05 ). In the radical gastrectomy for distal gastric cancer, carbon nanoparticle laparoscopy was not only helpful for the localization of small tumors but also for the thorough dissection of lymph nodes after the surgery, and the postoperative adverse reactions of carbon nanoparticle laparoscopy were also less, which was of great significance for the improvement of intestinal flora and the reduction of serum levels of EGFR, IL-32, and gastrin 17 in gastric cancer patients.

scholarly journals Computer Network Reliability Improvement Method Based on Genetic Algorithm

2021 ◽  
Vol 2066 (1) ◽  
pp. 012039
Author(s):  
Kun Huang
Keyword(s):  
Genetic Algorithm ◽  
Computer Network ◽  
Network Reliability ◽  
Control Group ◽  
Continuous Growth ◽  
Computing Industry ◽  
Network Operation ◽  
Improvement Method ◽  
Set Up ◽  
Experimental Group

Abstract With the continuous growth of economy and the continuous development of society, once the computer came out, it has been rapidly developed and popularized, especially now, people’s life is more and more inseparable from the computer, and they have a serious dependence on the computer. Some industries even directly use the computer as the business pillar, once such industries leave the computing industry Therefore, the reliability of computer network is a very important topic. It is very important to improve the reliability of computer network. In order to explore the effect of genetic algorithm on improving the reliability of computer network, two experimental groups are set up in this paper. The experimental group adds genetic algorithm in its computer network operation, while the control group uses the traditional algorithm. Finally, the experimental results show that the accuracy and security of the computer network in the experimental group are higher than those in the control group. For example, the highest accuracy and security of the experimental group are 99.78% and 98.83% respectively, while the highest accuracy and security of the control group are 84.31% and 88.58%. These experimental data show that the genetic algorithm can play a positive effect on the security of computer network.

Clinical study on harvesting lymph nodes with carbon nanoparticles for advanced gastric cancer: A prospective randomized trail.

2015 ◽  
Vol 33 (3_suppl) ◽  
pp. 159-159
Author(s):  
Ziyu Li ◽  
Sheng Ao ◽  
Jiafu Ji
Keyword(s):  
Gastric Cancer ◽  
Lymph Nodes ◽  
Advanced Gastric Cancer ◽  
Carbon Nanoparticles ◽  
Control Group ◽  
Solution Methods ◽  
The Mean ◽  
Set Up ◽  
Experimental Group ◽  
Clinical Trial Information

159 Background: There were no ideal materials nor quantizing regime for surgeons’ training on harvesting lymph nodes in advanced gastric cancer(AGC). A prospective randomized trial on Carbon Nanoparticles (CNP),a kind of lymphatic tracers, was designed to find a solution. Methods: Patients with previously untreated resectable AGC were eligible for inclusion in this study. All patients were randomly allocated to two subgroups. In experimental group, total of 1.0 mL CNP was injected into the subserosa of stomach around the tumor before gastrectomy with D2 dissection performed. On the contrary, the same procedure was performed directly without any coloring materials in control arm. Following surgery,the investigator harvested lymph nodes (LNs), counted colored LNs and measured the diameters with pathologist. Results: 30 patients were enrolled in the study. We observed no serious adverse effects related to CNP injection. The rate of stained LNs was 46.6%. The mean number of harvested LNs was larger in experimental than control group (38.33 vs. 28.27, p=0.041). Smaller diameter was recorded in experimental arm (3.32vs4.30mm,p=0.023), which might clarify the reason why the number of LNs was larger with CNP. In addition, we set up a model for predicting total number of LNs based on the data of CNP-staining LNs and metastatic LNs(MLNs), which might help the surgeons review their work of removing LNs so as to improve their surgical skills. Conclusions: CNP was a kind of safe materials and surgeons could harvest more LNs with it in AGC, which might benefit from the harvest of more smaller ones. Further study was needed to prove the model’s practicability. Clinical trial information: NCT02123407.

On Effects of Paternal Ethanol Treatment on Fetal Outcome

1985 ◽  
Vol 57 (1) ◽  
pp. 51-57 ◽  
Author(s):  
H. Cake ◽  
I. Lenzer
Keyword(s):  
Litter Size ◽  
Fetal Outcome ◽  
Ethanol Administration ◽  
Control Group ◽  
Ethanol Treatment ◽  
Sprague Dawley ◽  
Water Control ◽  
Sprague Dawley Rats ◽  
Experimental Group ◽  
Low Dosage

To study the effects of paternal chronic low-dosage ethanol administration on fetal outcome, male Sprague-Dawley rats received either .6g/ kg ethanol (experimental group) or water (control group). Males were mated between the fourth and seventh weeks of treatment. Pregnancies were terminated on gestational Day 21. Cerebral weight and placental weight in offspring of the ethanol-treated males were significantly larger than those of control males. There was no effect on litter size. Experimental females (dams that became pregnant when paired with an ethanol-treated male) were significantly heavier than control dams. Since these pairings were random at every mating, the experimental males appeared to have been more successful impregnating heavier than lighter females.

scholarly journals Food safety of plasma-chemically activated water and bread made with its use

2021 ◽  
Vol 6 (11 (114)) ◽  
pp. 74-83
Author(s):  
Svitlana Mykolenko ◽  
Olexandr Pivovarov ◽  
Valentyn Yefimov ◽  
Nataliia Sova ◽  
Dmytro Tymchak
Keyword(s):  
Food Safety ◽  
Water Treatment ◽  
Raw Materials ◽  
The Body ◽  
Wheat Bread ◽  
Control Group ◽  
Water Control ◽  
Temperature Plasma ◽  
Significant Difference ◽  
Experimental Group

Water treatment by contact non-equilibrium low-temperature plasma refers to innovative methods of processing food raw materials, which requires determining the level of safety of its use to meet the requirements of food safety for humans. The test animals were divided into four test groups with two different basic diets. The tested substances were drinking main water (control group) and plasma-chemically activated water (experimental group); wheat bread (control group) and wheat bread produced using plasma-chemically activated water (experimental group). It was found that there was no significant difference between the control and experimental groups of animals in body weight and its changes during 90 days of the introduction of the test substances into the diet. The amount of water and feed consumed was in direct proportion to the change in the weight of animals in the corresponding diets. The hematological and biochemical analysis of the blood of the test rats did not confirm the toxic or allergenic effect of the studied feeding factors on their organism. An increase in the number of erythrocytes in the blood and activation of the phagocytic activity of experimental groups of animals were confirmed. This confirms the positive effect of plasma-chemically activated water and wheat bread made with its use on metabolic processes in the body of animals. Macromorphological parameters of the body of animals and the results of histological studies of the stomach, liver, kidneys and femur as potential target organs demonstrated the absence of dystrophic-degenerative changes in these organs. A comprehensive study of the food safety of plasma-chemically activated water and wheat bread made with its use proves the possibility of using an innovative method of water treatment in food production.

scholarly journals APPLICATION OF FEED ADDITIVE BAFASAL IN POULTRY

2020 ◽  
Vol 21 (1) ◽  
pp. 126-129
Author(s):  
I. M. Kushnir ◽  
I. S. Semen ◽  
S. D. Murska ◽  
G.. V. Kolodiy
Keyword(s):  
Weight Gain ◽  
Antimicrobial Agents ◽  
Intestinal Flora ◽  
New Drugs ◽  
Daily Weight Gain ◽  
Feed Additive ◽  
Control Group ◽  
Feed Conversion ◽  
And Control ◽  
Experimental Group

Todays is not possible to overcam the problem of the resistance of microorganisms to antibiotics by the development of new antimicrobial agents. Because the new drugs development is quite long process, and the addiction of microorganisms to the antibiotics occurs very fast, in resulting microorganisms become resistant. Prospective direction in solving the problem of antibiotic resistance is the use of bacteriophages, which lytically acting on the pathogens, not causing any toxic effect on the macroorganism. In the article highlight the use of the feed additive Bafasal, which was made on the basis of a specific bacteriophage to serovars of Salmonella, in particular S. enteritidis, S. typhi, S. paratyphi, S. typhimurium, S. branderburg, S. hadar. Poultry of the experimental group were used Bafasal from 1 to 14 days of the experiment, from the calculation on 10 chickens - 50 cm3 of feed additive dissolved to 0,5 % concentration, and from 15 to 35 days - 100 cm3 of Bafasal dissolved to 0,25 % concentration. As a result in the conducted researches was found out that Bafasal causes an increase of weight gain of broilers, feed conversion. In particular, on the 35th day of the experiment, the average daily weight gain of the chickens of the experimental group was on 4,5 % higher than in broilers of the control group, and the conversion of feed in the experimental and control chicken was 1.74 and 1.93 units, respectively. Herewith, in broilers of the control group were not isolated pathogenic microorganisms, including salmonella. In determining the influence of the feed additive on some representatives of the intestinal flora of broilers was found that, the use of feed additive didnt cause changes in total amount lactic acid and bifidobacteria, in broiler of test and control groups, in the glandular, muscular stomach, duodenum, cecum and colon.

Instrumental Responding following Reinforcer Devaluation

1981 ◽  
Vol 33 (2b) ◽  
pp. 109-121 ◽  
Author(s):  
Christopher D. Adams ◽  
Anthony Dickinson
Keyword(s):  
Lever Press ◽  
Extinction Test ◽  
Control Group ◽  
Food Aversion ◽  
Associative Structure ◽  
Lever Pressing ◽  
Reinforcer Devaluation ◽  
Set Up ◽  
Experimental Group

In two experiments, hungry rats were given instrumental lever-press training for an appetitive reinforcer and, in addition, were exposed to another type of food which was not contingent on lever pressing. In the first experiment, exposure to each type of food was on separate days, whereas in the second experiment rats were exposed to each type of food in strict alternation within each session. Subsequently, a food aversion was conditioned to the reinforcer for the experimental group and to the non-contingent food for the control group. In both experiments, animals with an aversion to the reinforcer responded less in an extinction test than animals with an aversion to the non-contingent food. Subsequent reacquisition tests confirmed that the aversion to the non-contingent food in the control group was of comparable strength with that to the reinforcer in the experimental group. The results were discussed in terms of whether the reinforcer is encoded in the associative structure set up by exposure to an instrumental contingency.

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