scholarly journals Dracaena arborea (Dracaenaceae) Increases Sexual Hormones and Sperm Parameters, Lowers Oxidative Stress, and Ameliorates Testicular Architecture in Rats with 3 Weeks of Experimental Varicocele

2021 ◽  
Vol 2021 ◽  
pp. 1-12
Author(s):  
Yannick Baudouin Tchatat Petnga ◽  
Aimé Césaire Tetsatsi Momo ◽  
Modeste Wankeu-Nya ◽  
Désiré Munyali Alumeti ◽  
Georges Roméo Bonsou Fozin ◽  
...  
Keyword(s):  
Aqueous Extract ◽  
Sperm Quality ◽  
Ethanolic Extract ◽  
Male Rats ◽  
Sexual Hormones ◽  
Organ Weights ◽  
Sperm Density ◽  
Pampiniform Plexus ◽  
Antioxidant Markers ◽  
Antioxidant Effects

Varicocele is a disease characterized by an abnormal dilation of the pampiniform plexus that drains the testis. The main objective of this work was to evaluate the curative effects of aqueous and ethanolic extracts of Dracaena arborea on some reproductive and antioxidant markers in rats with experimental varicocele. Following varicocele induction, rats (5 per group) were randomly partitioned into untreated varicocele, vitamin E-treated (150 mg/kg), aqueous extract-treated (500 mg/kg), and ethanolic extract-treated (100 mg/kg) animals. Two other groups served as normal and sham-operated. After 2 or 4 weeks of treatments, body and sex organ weights, spermatozoa characteristics, antioxidant status, NO level, sex hormones, and testis histology were measured. Animals with 3 weeks of varicocele showed a significant ( p  < 0.05–0.001) decrease in body and sex organ weights, total proteins, sperm characteristics, testosterone concentration, SOD, catalase, and total peroxidase activities. An increase in the plasmatic FSH, LH, and testicular MDA and NO concentrations was also recorded. Moreover, marked disorganization of the testicular architecture was observed. Treatment with D. arborea significantly reversed these impairments due to varicocele. For instance, after 4 weeks, treatment with aqueous extract of D. arborea significantly ( p  < 0.05–0.001) increased testes and epididymis weights, sperm viability (89.12 ± 1.09 vs 68.22 ± 1.42), sperm density (148.50 ± 2.59 vs 110.25 ± 2.51), and sperm motility (68.16 ± 2.39 vs 55.88 ± 3.20) in the left side, compared with varicocele-untreated rats. The extract also significantly ( p  < 0.05–0.001) decreased malondialdehyde level (2.19 ± 0.04 vs 3.50 ± 0.13) but elevated catalase (0.97 ± 0.03 vs 0.55 ± 0.03), SOD (0.5 ± 0.03 vs 0.15 ± 0.03), and peroxidase (65.80 ± 2.9 vs 40.95 ± 2.44) activities. Present results showed that D. arborea extracts possess antioxidant effects and improve sperm quality in male rats with an existing varicocele.

Fluoxetine treatment of prepubertal male rats uniformly diminishes sex hormone levels and, in a subpopulation of animals, negatively affects sperm quality

2018 ◽  
Vol 30 (10) ◽  
pp. 1329 ◽  
Author(s):  
María E. Ayala ◽  
Ayari Gonzáles ◽  
Rodrigo M. Olivarez ◽  
Andrés Aragón-Martínez
Keyword(s):  
Adult Stage ◽  
Sperm Quality ◽  
Membrane Integrity ◽  
Graphical Analysis ◽  
Quality Parameters ◽  
Male Rats ◽  
Serum Concentrations ◽  
Sperm Density ◽  
Sexual Steroids ◽  
Fluoxetine Treatment

Fluoxetine (Flx) is a selective serotonin reuptake inhibitor that alters the male reproductive system when administered at the adult stage or after maternal exposure. In the present study we evaluated the effects of Flx administration on reproductive parameters during juvenile–peripubertal development when treated male rats reached adulthood. Groups of rats were treated daily with Flx (5 mg kg−1, i.p.) or saline (0.9% NaCl), or were left untreated. Rats were treated between 30 and 53 days of age and were killed at 65 days of age. Serotonin concentrations were determined in the hypothalamus, hypophysis and testis. Gonadotrophins, sex steroids and sperm quality (membrane integrity, sperm with functional mitochondria, sperm density, sperm motility and morphological abnormalities) were also evaluated. Flx did not affect bodyweight, but significantly diminished LH, FSH, progesterone and testosterone serum concentrations. After graphical analysis, a subgroup of rats was identified whose sperm quality parameters were greatly affected by Flx. In the present study we show that Flx administered to juvenile rats disrupts the hypothalamic–hypophyseal–testicular axis and its effects on sperm quality are not homogeneous in adults. In contrast, Flx altered concentrations of gonadotrophins and sexual steroids in all treated rats. These results suggest caution should be exercised in the prescription of Flx to prepubertal males.

scholarly journals Colibri® insecticide induces male reproductive toxicity: alleviating effects of Lannea acida (Anacardiaceae) in rats

2019 ◽  
Vol 29 (1) ◽  
Author(s):  
Aimé Césaire Momo Tetsatsi ◽  
Pepin Alango Nkeng-Effouet ◽  
Désiré Munyali Alumeti ◽  
Georges Roméo Fozin Bonsou ◽  
Albert Kamanyi ◽  
...  
Keyword(s):  
Vitamin E ◽  
Preventive Measures ◽  
Clomiphene Citrate ◽  
Reproductive Toxicity ◽  
Male Rats ◽  
Sexual Hormones ◽  
Organ Weights ◽  
Stress Markers ◽  
Testis Histology ◽  
Neonicotinoid Insecticide

Abstract Background Imidacloprid, a neonicotinoid insecticide, has been associated to severe reproductive toxicity in mammals. Although some preventive measures have been reported, curative strategies are yet to be explored. The present study was designed to investigate the alleviating effects of Lannea acida on the reproductive toxicity of colibri®, a commercial formulation of imidacloprid, in adult male rats. Materials and methods Seventy rats were orally administered with colibri® (22.5 mg/kg, 10 mL/kg) for 14 days and treated for other 14 or 28 days with either aqueous or methanol extracts of L. acida (170 or 340 mg/kg). Control animals were similarly treated with clomiphene citrate or vitamin E. Sexual organ weights, spermatozoa characteristics, sexual hormones, stress markers and testis histology were evaluated at the end of each treatment period. Results Colibri® exposition induced reproductive toxicity marked by a decrease in sex organ weights, spermatozoa count, motility and viability. Colibri® also decreased testosterone, luteinizing hormone, follicle stimulating hormone concentrations and increased testicular oxidative stress. Spermatozoa morphology and testis histology were also severely altered. Similar to clomiphene citrate and vitamin E, treatment with L. acida extracts significantly (p ≤ 0.05–0.001) reversed the above-mentioned damages, especially after 28 days of treatment with aqueous (340 mg/kg) and methanol (170 mg/kg) extracts. Conclusion Present results indicate that L. acida exerts curative effects against colibri®-induced male reproductive toxicity. These results justify the use of this plant as fertility enhancer and suggest that it could be an alternative in the management of pesticide-derived male infertility.

scholarly journals The Potential Effect of Gynura Procumbens Aqueous Extract as Anti-Hyperglycaemia, Pro-Fertility and Libido Agent Towards Diabetes-Induced Male Rats

2020 ◽  
Vol 3 ◽  
pp. 103-108
Author(s):  
Khaidatul Akmar ◽  
Mahanem Mat Noor
Keyword(s):  
Blood Glucose ◽  
Beneficial Effect ◽  
Aqueous Extract ◽  
Sperm Quality ◽  
Treated Group ◽  
Positive Control ◽  
Male Rats ◽  
Seminiferous Tubules ◽  
Control Group ◽  
Control Groups

Diabetes mellitus is a metabolic disorder that often affected fertility health, specifically in men. Gynura procumbens (G. procumbens) has been used as traditional medicine due to its ability in treating various types of illness. Hence, this study was carried out to determine the potential beneficial effect of G. procumbens as anti-hyperglycaemia, pro-fertility and libido agent towards diabetes-induced male rats. A total of 42 male Sprague Dawley rats were randomly assigned into six groups; normal, negative, and positive control, and three treated groups of different dosages of G. procumbens aqueous extract (GPAE); 150 mg/kg, 300 mg/kg and 450 mg/kg. Each group was given treatment via oral gavage for seven consecutive days. The rats were sacrificed on day eighth for further analysis. The fasting blood glucose (FBG) level in all GPAE treated group showed significant decrease, and 450 mg/kg treated group showed significant reduction to a normal blood glucose level compared to all groups. There were significant increases in the sperm quality of GPAE treated groups after seven days of treatment compared to the negative and positive control group, specifically in 450 mg/kg treated group. Testis histology showed that GPAE treated groups produced a significant result whereby the seminiferous tubules were seen packed with sperm and successive stage of spermatogenesis were shown compared to control groups. Hormone analysis suggested that the luteneizing hormone (LH), follicle stimulating hormone (FSH) and testosterone hormone of the treated groups were elevated after seven days of treatment compared to the negative and positive control groups. Libido analysis of seven days treatment showed that GPAE treated groups, significantly improved in 450 mg/kg dose compared to other groups, with the highest number of mounting frequency and shortest mounting latency. Fertility test revealed a significant increase in number of implantation sites produced in GPAE treated groups compared to positive and negative control groups. In conclusion, GPAE exhibited a potential beneficial effect as an anti-diabetic and also as fertility agent in diabetic-induced male rats.

scholarly journals Effect of Curcuma longa Aqueous Extract on Male Fertility in Aluminum Exposed Wistar Rats

2020 ◽  
Vol 10 (5) ◽  
pp. 11-17
Author(s):  
Farouk BOUDOU ◽  
Malika BENDAHMANE-SALMI ◽  
Mokhtar BENABDERRAHMANE ◽  
Amal BELAKREDAR ◽  
Abdelkrim BENALIA ◽  
...  
Keyword(s):  
Aqueous Extract ◽  
Sperm Quality ◽  
Curcuma Longa ◽  
Oral Treatment ◽  
Sperm Concentration ◽  
Serum Testosterone ◽  
Interstitial Cells ◽  
Male Rats ◽  
Seminiferous Tubules ◽  
Morphological Abnormalities

To assess the effects of Curcuma longa aqueous extract (CE) on fertility in male rats exposed to Aluminum chloride (AlCl3), Twenty four male rats, 3 months old, divided into 4 groups (n=6) were used. Animal from the first group received, by the oral route, water-containing AlCl3 at the dose of 34 mg/kg body weight (BW). Rats from the second group received both AlCl3 at the same dose in combination with oral treatment of CE at a dose of 200 mg/kg BW. The third group received only an oral administration of CE with the same dose. Rats from the fourth group, without any treatment, served as control. After 4 weeks of experimentation, AlCl3 exposure showed a significant decrease in sperm concentration (4.58±0.65 × 106 cells /ml), and percentage of viability (61.53±23.63 %), and an increase of morphological abnormalities (26.11±17.84 %). A significant decreased in serum testosterone levels (0.31±0.26 ng/ml) and an increase of testicular malondialdehyde (MDA) level (0.16±0.015 μM/g) were also observed. Histological examination of the testes showed degeneration of the seminiferous tubules, germ line cells, and interstitial cells. However, CE treatment concomitant to AlCl3 showed that the rate of morphological abnormalities (19±2.65 %) is significantly decreased compared to AlCl3 group, with a significant increase in serum testosterone (1.17±0.24 ng/ml) and a significant decrease in MDA (0.11±0.003μM/g) level. Microscopic examination revealed a significant regeneration of seminiferous tubules and interstitial cells. This study demonstrated an ameliorative effect of Curcuma longa aqueous extract in testicular tissue and sperm quality. Keywords: Curcuma longa, Aluminum, fertility, testosterone, malondialdehyde (MDA), sperm.

scholarly journals Mass spectrometry data of diabetic rat sperm proteome treated with Gynura procumbens aqueous extract

F1000Research ◽  
2019 ◽  
Vol 8 ◽  
pp. 844
Author(s):  
Khaidatul Akmar Kamaruzaman ◽  
Wan Mohd Aizat ◽  
Mahanem Mat Noor
Keyword(s):  
Mass Spectrometry ◽  
Aqueous Extract ◽  
Sperm Quality ◽  
Mass Spectrometry Data ◽  
Male Rats ◽  
Diabetic Rat ◽  
Control Group ◽  
Label Free ◽  
Sprague Dawley ◽  
Proteomics Data

Diabetes mellitus has a deleterious effect on the male reproductive system, especially on sperm quality and spermatogenesis. Gynura procumbens (G. procumbens) is a traditional herb known for its ability to improve the fertility of diabetes-induced male rats. This study was designed to identify the differential expression of sperm proteins after treatment with G. procumbens aqueous extract on diabetes-induced male rats. The sperm proteome was profiled using label-free shotgun proteomics analysis. Sprague Dawley rats used in this study were divided randomly into four groups. One group was a normal control group (healthy rats), while the three other groups were induced with 50 mg/kg  bodyweight (BW) of streptozotocin (STZ) to emulate the diabetic condition. The diabetic rats were divided into negative control (non-treated diabetic), metformin-treated (positive control) and G. procumbens aqueous extract-treated (450 mg/kg BW) groups. Oral treatments were administered for 14 consecutive days before the rats were euthanized. Total sperm protein samples were extracted from the caudal epididymis and run through SDS-PAGE. Later, samples were digested using trypsin before liquid chromatography-tandem mass spectrometry (Thermo Orbitrap Fusion) analysis. The acquired data were processed using MaxQuant and Perseus software. The mass spectrometry proteomics data is available through ProteomeXchange Consortium via the PRIDE partner repository, with the dataset identifier PXD011373.

Aphrodisiac Activity of an Aqueous Extract of Wood Ear Mushroom, Auricularia polytricha (Heterobasidiomycetes), in Male Rats

2018 ◽  
Vol 20 (1) ◽  
pp. 81-88 ◽  
Author(s):  
Gaurav Gupta ◽  
Rakesh Kumar Sharma ◽  
Rajiv Dahiya ◽  
Anurag Mishra ◽  
Juhi Tiwari ◽  
...  
Keyword(s):  
Aqueous Extract ◽  
Male Rats ◽  
Auricularia Polytricha

scholarly journals Numerical Modeling of Sperm Swimming

Fluids ◽  
2021 ◽  
Vol 6 (2) ◽  
pp. 73
Author(s):  
Fang-Bao Tian ◽  
Li Wang
Keyword(s):  
Sperm Quality ◽  
Computational Studies ◽  
Theoretical Methods ◽  
Structure Interaction ◽  
Sperm Density ◽  
Fluid Environment ◽  
Complex Fluid ◽  
Fluid Rheology ◽  
Wall Interactions ◽  
Open Issues

Due to rising human infertility, sperm motility has been an important subject. Among the hundreds of millions of sperms on the journey up the oviducts, only a few excellent travelers will reach the eggs. This journey is affected by many factors, some of which include sperm quality, sperm density, fluid rheology and chemotaxis. In addition, the sperm swimming through different body tracks and fluids involves complex sperm flagellar, complex fluid environment, and multi-sperm and sperm-wall interactions. Therefore, this topic has generated substantial research interest. In this paper, we present a review of computational studies on sperm swimming from an engineering perspective with focus on both simplified theoretical methods and fluid–structure interaction methods. Several open issues in this field are highlighted.

Evaluation of epididymal sperm quality following experimentally induced copper poisoning in male rats

Andrologia ◽  
2011 ◽  
Vol 44 ◽  
pp. 110-116 ◽  
Author(s):  
E. Sakhaee ◽  
L. Emadi ◽  
J. Abshenas ◽  
R. Kheirandish ◽  
O. Azari ◽  
...  
Keyword(s):  
Sperm Quality ◽  
Male Rats ◽  
Epididymal Sperm ◽  
Copper Poisoning ◽  
Experimentally Induced

A Study on the Effect of Samoa Extract (Cleome droserifolia) on Sperm Quality in Male Albino Rats Exposed to Pyrethroid

2021 ◽  
pp. 39-45
Author(s):  
Nura I. Al-Zail ◽  
Salah F. Kamies
Keyword(s):  
Sperm Quality ◽  
Group Iv ◽  
Male Rats ◽  
Control Group ◽  
Albino Rats ◽  
Protective Agent ◽  
Group V ◽  
Group Iii ◽  
Group I ◽  
Induced Changes

Pyrethroid cyhalothrin (PC) is an insecticide that is used worldwide for pest control in agriculture and household use. Samoa extract (SE) is a potent antioxidant protecting cells from oxidative stress. The present study investigates the protective and therapeutic effect of SE on PC-induced changes in sperm quality in male rats. Fifty adult male albino rats were divided into five groups: group I: served as control; group II: received PC i.p. only (6.2 mg/kg b.wt.); group III: received SE only (100 mg/kg b.wt., p.o.) for eight weeks; group IV: received SE as a protective agent daily for eight weeks, then followed by the administration of PC (i.p.) three times a week for two weeks; group V: exposed to PC (i.p.) three times a week for two weeks, then treated with the SE daily for 8 weeks. Results showed that PC caused markedly impaired sperm quality (a count, viability, motility, and abnormality). Compared to PC-treated animals, SE in the protective group markedly restored the alteration of sperm indices. However, SE in the curative group was found to be less effective in restoring PC-induced alterations. In conclusion, the data of this study revealed that the SE as a protective agent is more effective than as a therapeutic agent. Keywords: Samoa; Pyrethroid; Sperm quality; Rat

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