Research on Biological Molecular Mechanism of Mesenchymal Stem Cells (MSCs) in Oral Squamous Cell Carcinoma (OSCC) Through miR-34a

2021 ◽  
Vol 11 (12) ◽  
pp. 2454-2459
Author(s):  
Jing Qi ◽  
Guang Chen ◽  
Xiaojun Wang ◽  
Haiqiong Ran ◽  
Haiquan Yue
Keyword(s):  
Risk Factor ◽  
Independent Risk Factor ◽  
Tissue Sample ◽  
Apoptotic Cell ◽  
Cell Activity ◽  
Life Time ◽  
Control Group ◽  
Qrt Pcr ◽  
Oral Tissue ◽  
Novel Target

This study aims to assess the action of miR-34a on MSCs homing onto OSCC. Sixty cases of tissue sample in OSCC was analyzed retrospectively and thirty of normal oral tissue was included as a control group. miR-34a expression in OSCC was detected by qRT-PCR. The relation of expression with OSCC was analyzed. The independent risk factor in the life time of OSCC was studied. pc-DNA-miR-34a and si-miR-34a was transfected into MSCs to analyze its action on MSCs homing onto OSCC by CCK-8 and clone formation assays as well as on cell apoptosis by flow cytometry. The level of miR-34a in patients with OSCC was significantly elevated and was associated with overall survival and prognosis. The FIGO staging and miR-34a expression was the independent risk factor of DFS and OS of patients with OSCC. The cell activity and homing capacity of MSCs was reduced significantly after knock-down of miR-34a expression along with increased apoptotic cell rate. In addition, the miR-34a could prompt the MSC homing onto OSCC. In conclusion, miR-34a level was up-regulated significantly in OSCC and associated with the prognosis, indicating that it might be a novel target for the treatment of OSCC.

scholarly journals Association between serum lipid and ischaemic stroke in a tertiary hospital in Northern Andhra Pradesh, India

2020 ◽  
Vol 7 (7) ◽  
pp. 1078
Author(s):  
Tamminana Venugopala Rao ◽  
Budumuru Annaji Rao ◽  
Sreedevi Panchadi ◽  
K. Sudheer
Keyword(s):  
Risk Factor ◽  
Ischaemic Stroke ◽  
Serum Lipid ◽  
Serum Lipids ◽  
Independent Risk Factor ◽  
Ldl Cholesterol ◽  
Control Group ◽  
Case Group ◽  
Stroke Patients ◽  
The Brain

Background: The incidence of cerebrovascular disease increases with age and the number of strokes is projected to increase as the elderly population grows. A stroke occurs when blood vessels that carry blood to the brain suddenly blocked or burst, preventing blood flow to the brain. The most common cause of blood vessel blockages is thrombosis (a blood clot) or an embolism (floating clot). Blood clots may form in the arteries that are damaged by atherosclerosis. Atherosclerosis is an aging process but some factors (risk factor) precipitate it to occur earlier. To find out the risk factors properly are of tremendous importance as risk factor change could directly influence or indirectly affect case fatality by altering the natural history of the disease. Serum lipids are thought to interact with the pathogenesis of stroke through the atherosclerotic mechanism. Objective was to identify the high serum lipid as an independent risk factor of stroke.Methods: This is a hospital-based case-control study. Seventy cases of stroke patients and age, sex-matched 70 healthy control subjects were enrolled by non-random sampling. 12 hours of fasting plasma lipids were estimated in both cases and control subjects. Then it was compared between cases and controls.Results: Hypercholesterolemia was higher in the case group than control but not statistically significant. Mean LDL- cholesterol, and triglycerides were significantly higher in the case group than the control group. The mean value of serum HDL-cholesterol was not significantly lower in the case group than the control group.Conclusions: Serum lipids are significantly higher in ischaemic stroke patients than the control group (LDL cholesterol and triglyceride). So, it may be an independent risk factor of ischemic stroke.

scholarly journals Risk factors for post-bronchoscopy pneumonia: a case–control study

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Yu Sato ◽  
Kengo Murata ◽  
Miake Yamamoto ◽  
Tsukasa Ishiwata ◽  
Miyako Kitazono-Saitoh ◽  
...  
Keyword(s):  
Risk Factors ◽  
Multivariate Analysis ◽  
Risk Factor ◽  
Independent Risk Factor ◽  
Case Control Study ◽  
Case Control ◽  
Control Group ◽  
Tracheobronchial Stenosis ◽  
Control Study ◽  
Age And Sex

AbstractThe bronchoscopy, though usually safe, is occasionally associated with complications, such as pneumonia. However, the use of prophylactic antibiotics is not recommended by the guidelines of the British Thoracic Society. Thus far there are few reports of the risk factors for post-bronchoscopy pneumonia; the purpose of this study was to evaluate these risk factors. We retrospectively collected data on patients in whom post-bronchoscopy pneumonia developed from the medical records of 2,265 patients who received 2666 diagnostic bronchoscopies at our institution between April 2006 and November 2011. Twice as many patients were enrolled in the control group as in the pneumonia group. The patients were matched for age and sex. In total, 37 patients (1.4%) had post-bronchoscopy pneumonia. Univariate analysis showed that a significantly larger proportion of patients in the pneumonia group had tracheobronchial stenosis (75.7% vs 18.9%, p < 0.01) and a final diagnosis of primary lung cancer (75.7% vs 43.2%, p < 0.01) than in the control group. The pneumonia group tended to have more patients with a history of smoking (83.8% vs 67.1%, p = 0.06) or bronchoalveolar lavage (BAL) (4.3% vs 14.9%, p = 0.14) than the control group. In multivariate analysis, we found that tracheobronchial stenosis remained an independent risk factor for post-bronchoscopy pneumonia (odds ratio: 7.8, 95%CI: 2.5–24.2). In conclusion, tracheobronchial stenosis was identified as an independent risk factor for post-bronchoscopy pneumonia by multivariate analysis in this age- and sex- matched case control study.

scholarly journals Effect of Glycemic Control on Right Heart Functions in Type 2 Diabetes Mellitus Patients Free of Clinical Cardiovascular Disease

2020 ◽  
Author(s):  
Murat Ziyrek
Keyword(s):  
Diabetes Mellitus ◽  
Risk Factor ◽  
Glycemic Control ◽  
Negative Correlation ◽  
Hba1c Level ◽  
Independent Risk Factor ◽  
Control Group ◽  
Type 2 Dm ◽  
Ventricle Function

Diabetes mellitus (DM) is one of the most common endocrine disorders. 90% percent of all diabetics are diagnosed with type 2 DM. DM is closely associated with various vascular diseases, and successful glycemic control prevents micro and macrovascular complications. Although there is data about the relation between glycemic control and left ventricle function, there is hardly any data about the relation between the right ventricular function. We analyzed the relationship between glycemic control and right ventricle function in type 2 DM patients free of clinical cardiovascular diseases (CVD). Patients were selected from the cardiology outpatient clinic. 53 patients formed the DM group; 51 patients formed the control group. All patients' demographic data were recorded. Biochemical tests and echocardiographic examinations were performed. RA and RV diameters were significantly higher in DM group (3.36±0.32 vs 3.13±0.34, P=0.015; 2.80±0.32 vs 2.56±0.22 P=0.005 respectively). Myocardial velocity during isovolumetric contraction (RV/IVV) and myocardial acceleration during Isovolumetric contraction (RV/IVA) were significantly lower in the DM group (14.4±3.17 vs 16.04±4.13 P=0,019; 3.25±0.75 vs 3.95±1,25 P=0.015). There was an intermediate negative correlation between RV/IVV and HBA1C (r=-0.406; P=0.036). HBA1C level was an independent risk factor for RV IVV (β=-0.406; P=0.036). It is shown that RA, RV diameter were significantly higher; RV/IVV and RV/IVA were significantly lower in diabetes mellitus patients free of CVD. Furthermore, there was a significant negative correlation between RV/IVV and HBA1C levels. HBA1C level was an independent risk factor for RV/IVV.

scholarly journals Increased Serum Angiotensin II Is a Risk Factor of Nonalcoholic Fatty Liver Disease: A Prospective Pilot Study

2019 ◽  
Vol 2019 ◽  
pp. 1-7
Author(s):  
Yue Li ◽  
Feng Xiong ◽  
Wen Xu ◽  
Side Liu
Keyword(s):  
Risk Factor ◽  
Liver Disease ◽  
Angiotensin Ii ◽  
Fatty Liver Disease ◽  
Independent Risk Factor ◽  
Abdominal Ultrasound ◽  
Control Group ◽  
Ang Ii ◽  
Nonalcoholic Fatty Liver ◽  
And Control

Background and Aims. Nonalcoholic fatty liver disease (NAFLD) is one of the most prevalent chronic liver diseases. In this prospective study, we aim to explore the role of angiotensin II (Ang II) and NLRP3 inflammasome in NAFLD patients. Methods. We prospectively enrolled 96 patients in our hospital from September 2014 to February 2016. Patients were divided into two groups (NAFLD group and Control group), and the serum Ang II level, IL-1β, IL-18, and lipids were analyzed. Correlation and multivariable analyses were used in order to identify the potential risk factors of NAFLD. Results. Although the two groups share a similar demographic background, the Ang II level of NAFLD group patients was significantly higher than that of the Control group (42.18±12.37 vs. 36.69±13.90, p=0.014) when abdominal ultrasound was used for grouping. This finding was confirmed when a FibroScan Cap value was selected to divide participants into the NAFLD group and Control group (41.16±13.06 vs. 34.85±12.64, p=0.040). Multivariable analysis showed that Ang II level is an independent risk factor of NAFLD whether abdominal ultrasound (OR=1.056, p=0.037) or FibroScan Cap value (OR=1.069, p=0.013) was deemed as the diagnostic standard. Furthermore, stepwise regression analysis was carried out between Ang II with other parameters and we discovered that Ang II had a linear correlation with IL-1β. Conclusion. Ang II levels of NAFLD patients significantly increased, and elevated Ang II level is an independent risk factor of NAFLD. Our preliminary results also indicate that Ang II may promote the development of NAFLD by activating NLRP3 inflammasome.

scholarly journals Liver Transplant Patients with High Preoperative Serum Bilirubin Levels Are at Increased Risk of Postoperative Delirium: A Retrospective Study

2020 ◽  
Vol 9 (5) ◽  
pp. 1591
Author(s):  
Kyu Hee Park ◽  
Hyo Jung Son ◽  
Yoon Ji Choi ◽  
Gene Hyun Park ◽  
Yoon Sook Lee ◽  
...  
Keyword(s):  
Risk Factor ◽  
Bilirubin Level ◽  
Independent Risk Factor ◽  
Postoperative Delirium ◽  
Serum Bilirubin ◽  
Meld Score ◽  
Risk Indicators ◽  
Control Group ◽  
Preoperative Serum ◽  
Increased Risk

Postoperative delirium is a common complication after liver transplantation (LT). A high model for end-stage liver disease (MELD) score is an independent risk factor for postoperative delirium, but it is unclear which of the components of this score are risk indicators. The aim of this study was to analyze the incidence of postoperative delirium according to the preoperative serum bilirubin level, a component of the MELD score, in patients who underwent LT. The medical records of 325 patients who underwent LT from January 2010 to February 2019 at a single university hospital were retrospectively reviewed. The patients were divided into two groups: those who experienced postoperative delirium (Delirium group, n = 69) and those who did not (Control group, n = 256). Data on the patients’ demographic characteristics, perioperative management, and postoperative complications were collected. Mean preoperative bilirubin level was higher in the Delirium group than in the Control group (p < 0.0001). In the Delirium group, 54 (78.26%) patients had preoperative bilirubin levels above 3.5 mg/dL. In the multivariate analysis, preoperative bilirubin above 3.5 mg/dL was associated with postoperative delirium (p = 0.002). Therefore, preoperative hyperbilirubinemia is an independent risk factor for postoperative delirium.

scholarly journals Alcohol as an independent risk factor for obstructive sleep apnea

2021 ◽  
Author(s):  
Shanshan Yang ◽  
Xinhong Guo ◽  
Wei Liu ◽  
Yanhua Li ◽  
Yunxi Liu
Keyword(s):  
Obstructive Sleep Apnea ◽  
Risk Factor ◽  
Sleep Apnea ◽  
Alcohol Consumption ◽  
Independent Risk Factor ◽  
Control Group ◽  
Logistic Regression Models ◽  
Obstructive Sleep ◽  
The Relationship ◽  
Control Study

Abstract Background To determine if alcohol consumption is a risk factor for obstructive sleep apnea (OSA) and nocturnal oxygen desaturation. Methods This case–control study evaluated patients with confirmed OSA and a control group using polysomnography (PSG). Two doctors who have worked in this field for more than 5 years provided a blinded interpretation of the patients’ monitoring results. Logistic regression models were used to identify the odds ratio (OR) for alcohol consumption on OSA. Results A total of 793 patients were enrolled in this study. Compared with those who did not consume alcohol, those consuming alcohol had a higher risk of OSA (OR 2.03, 95% CI 1.30–3.17) after adjustment. Regarding the risk of OSA after adjusting for former drinkers and current ones, the ORs were 1.96 (95% CI 1.19–3.22) and 2.22 (95% CI 1.06–4.63), respectively. And the P for trend = 0.002. The β of former drinkers and the current ones were 3.448 and 4.560 after adjustment; P for trend was 0.006. The relationship may have gender difference, and alcohol consumption was associated with AHI in female significantly (β = 10.190 and 15.395 for former and current drinkers, respectively, in females after adjustment, P for trend = 0.002). Conclusions In this study, we found that alcohol consumption was an independent risk factor of OSA and OSA with hypoxia, and alcohol consumption was related to AHI significantly after adjustment, especially in female. In order to reduce the risk and severity of OSA, it is suggested that people should avoid drinking, and drinkers should abstain from drinking.

scholarly journals Study on Homocysteinemia as a Risk Factor of Ischemic Stroke

2021 ◽  
Vol 34 (1) ◽  
pp. 33-39
Author(s):  
Md Amjad Hossain Pramanik ◽  
Achinta Kumar Mallick ◽  
Mukul Kumar Sarkar ◽  
SM Emdadul Haque ◽  
Md Raseul Kabir ◽  
...  
Keyword(s):  
Risk Factors ◽  
Risk Factor ◽  
Ischemic Stroke ◽  
Independent Risk Factor ◽  
Homocysteine Level ◽  
Control Group ◽  
Age Group ◽  
Healthy Individuals ◽  
Serum Homocysteine ◽  
And Control

Despite recent advances, only two-third of all strokes can be attributed to known causal risk factors. Homocysteine (tHcy), a sulfur-containing amino acid, is now considered to be an important risk factor for vascular diseases, along with the established risk factors like hyperlipidemia, hypertension, diabetes mellitus, and smoking. Elevated homocysteine levels play a causal role in the pathogenesis of atherosclerosis, thromboembolism and vascular endothelial dysfunction with an increased incidence of ischemic stroke.  This study aimed to find out the association of hyperhomocysteinemia with ischemic stroke. A total of 100 subjects were included in this study, 50 were ischemic stroke patients enrolled as case, and 50 were normal healthy individuals enrolled as control. Serum homocysteine level was measured in both case and control groups. The comparison was made in both groups regarding other common risk factors like diabetes mellitus, hypertension, smoking, dyslipidemia, family history, etc.  Among 100 patients, 50 had ischemic stroke and 50 were healthy individuals. In this study, out of all patients, abnormal serum homocysteine level was found in 32% of cases and 12% of controls. The mean (±SD) serum homocysteine level was found 16.50±13.86 μmole/L in cases and 9.46±3.49 μmole /L in the control group. Significant (p<0.05) difference was found between the case and the control. The incidence of hyperhomocysteinemia is higher in ischemic stroke cases than that in age-sex-matched healthy controls. In our study, serum homocysteine was high in both younger age group patients (16.65±14.55 μmole/L vs. 9.52±3.19 μmole/L) and older age group patients (16.33±9.87 vs. 9.35±3.97 μmole/L,) in case and control group respectively. Significant (p<0.05) difference was found between the case and the control. Multiple logistic regression analysis showed that abnormal serum homocysteine is an independent risk factor of ischemic stroke. So we conclude that hyperhomocysteinemia is an important and independent risk factor for the development of ischemic stroke. Hypertension and smoking are important contributory to elevated serum homocysteine. TAJ 2021; 34: No-1: 33-39

scholarly journals Obesity: an independent risk factor for oxidative stress

2017 ◽  
Vol 4 (3) ◽  
pp. 718 ◽  
Author(s):  
Navneet Agrawal ◽  
Sanjeev Kumar Singh
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Risk Factor ◽  
Independent Risk Factor ◽  
Pearson Correlation ◽  
Randomized Study ◽  
Control Group ◽  
Male Predominance ◽  
Myocardial Oxidative Stress ◽  
And Control

Background: Obesity is increasing in prevalence and presents a serious risk for the development of various disorders like diabetes mellitus, hypertension, heart disease, gall bladder disease and certain forms of cancer. Animal studies have shown that obesity is associated with increased myocardial oxidative stress and increased lipid peroxidation. The objective of this study was to test the hypothesis that obesity per se causes increased plasma lipid peroxidation and decreased erythrocyte cytoprotection.Methods: A prospective randomized study including 300 obese subjects, was conducted in the Department of Medicine and Department of Biochemistry, G. R. Medical College, Gwalior. Age and sex matched 100 subjects having BMI between 19 to 25 kg/m2 were also enrolled. Patients were grouped as Case (n=300) and Control (n=100). Detailed physical examination and laboratory investigations including lipid profile were performed. Venous blood was obtained and used for the estimation of Superoxide dismutase (SOD) and plasma malondialdehyde (MDA). Unpaired t test and analysis of variance (ANOVA) with post-hoc Bonferroni and Tukey test along with Pearson correlation was used to analyze the data using IMB SPSS ver. 20 software. Significance is assessed at 5 % level.Results: Mean age of subjects among Case and Control group was 46.2±2.4 years and 44.5±2.2 years respectively with male predominance in each group. Mean weight, height, BMI, waist, hip, waist to hip ratio, mean blood glucose, total cholesterol, triglyceride, LDL-C, HDL-C and VLDL-C among Cases were91.57±9.8 kg, 161.6±9.3 cm, 36.17± 3.4 kgm2, 114.7±6.2 cm, 114.23±17.12 cm, 0.98±0.22, 87.3±2.6 mg/dl, 196.0±12.6 mg/dl, 253.6±27.3 mg/dl, 135±47.03 mg/dl, 47.1±1.2 mg/dl and 45.8±14.03 mg/dl and among Control group were61±5.2 kg, 163.1±8.7 cm, 21.24±1.88 cm, 21.24±1.88 kg m2, 85.2±1.4 cm, 97.32±9.12 cm, 0.86±0.14, 94.4 ± 3.2 mg/dl, 186.6 ± 6.9 mg/dl, 143.4±15.4 mg/dl, 95.73±27.48 mg/dl, 51.6±1.7 mg/dl and 22.4±10.45mg/dl respectively. Mean MDA level in Case and Control group was 4.68 ± 1.72 and 2.06±0.76 μmol/ml respectively (p< 0.001). Mean SOD level among Case and Control groups was 7.65±1.13 and 12.42±2.18 units/ml respectively (p<0.001). Female obese patients had lower level of SOD. A significant negative correlation of SOD was observed with BMI (n=300, r= -0.045, P<0.001), whereas, MDA was positively correlated with BMI (n=300, r= 0.342, P<0.001).Conclusions: Obesity in humans is an independent risk factor for lipid peroxidation and depletion of cytoprotective enzymes even in the absence of other confounding factors such as diabetes and hyperlipidaemia.

scholarly journals Midlife insulin resistance, APOE genotype, and late-life brain amyloid accumulation

Neurology ◽  
2018 ◽  
Vol 90 (13) ◽  
pp. e1150-e1157 ◽  
Author(s):  
Laura L. Ekblad ◽  
Jarkko Johansson ◽  
Semi Helin ◽  
Matti Viitanen ◽  
Hanna Laine ◽  
...  
Keyword(s):  
Insulin Resistance ◽  
Risk Factor ◽  
Confidence Interval ◽  
Independent Risk Factor ◽  
Late Life ◽  
Control Group ◽  
Model Assessment ◽  
Amyloid Accumulation ◽  
Increased Risk

ObjectiveTo examine whether midlife insulin resistance is an independent risk factor for brain amyloid accumulation in vivo after 15 years, and whether this risk is modulated by APOE ε4 genotype.MethodsThis observational study examined 60 elderly volunteers without dementia (mean age at baseline 55.4 and at follow-up 70.9 years, 55.5% women) from the Finnish population-based, nationwide Health2000 study with [11C]Pittsburgh compound B–PET imaging in 2014–2016. The participants were recruited according to their homeostatic model assessment of insulin resistance (HOMA-IR) values in the year 2000, and their APOE ε4 genotype. The exposure group (IR+, n = 30) consisted of individuals with HOMA-IR >2.17 at baseline (highest tertile of the Health2000 study population), and the control group (IR−, n = 30) consisted of individuals with HOMA-IR <1.25 at baseline (lowest tertile). The groups were enriched for APOE ε4 carriers, resulting in 50% (n = 15) APOE ε4 carriers in both groups. Analyses were performed with multivariate logistic and linear regression.ResultsAn amyloid-positive PET scan was found in 33.3% of the IR− group and 60.0% of the IR+ group (odds ratio 3.0, 95% confidence interval 1.1–8.9, p = 0.04). The increased risk was seen in carriers and noncarriers of APOE ε4 genotype. Higher midlife, but not late-life continuous HOMA-IR was associated with a greater brain amyloid burden at follow-up after multivariate adjustments for other cognitive and metabolic risk factors (β = 0.11, 95% confidence interval 0.002–0.22, p = 0.04).ConclusionsThese results indicate that midlife insulin resistance is an independent risk factor for brain amyloid accumulation in elderly individuals without dementia.

STUDY ON THE PREDICTION OF HYPERGLYCEMIA ACCORDING TO THE DURATION OF VEGAN DIET IN FEMALE

2017 ◽  
pp. 58-63
Author(s):  
Hai Quy Tram Nguyen ◽  
Formato Marilena ◽  
Hai Thuy Nguyen ◽  
Thi kim Anh Nguyen ◽  
Hai Ngoc Minh Nguyen
Keyword(s):  
Risk Factor ◽  
Key Words ◽  
Independent Risk Factor ◽  
Fasting Glucose ◽  
Vegetarian Diet ◽  
Vegan Diet ◽  
Control Group ◽  
Vegan Group ◽  
Hba1c Levels

Objectives: To determine the prediction of hyperglycemia according to the duration of vegan diet in female. Materials and Methods: 144 female vegans aged from 20-75 with duration of vegan diet ranged 1070 years, mean 27.8±15.9 years that were screened for carbohydrate dysmetabolism. They were compared with 68 women with aged from 22-84 non-vegetarian diet. Results: The average fasting glucose in female Vegan group was higher than in control group (5.00 ±1.40 vs 4.67 ± 0.98 mmol/l, p<0.05). The prevalence of hyperglycemia (based on fasting glucose) in female Vegan group was higher than in control group (13.2% vs 10.3%, p < 0.05). Especially, there were significant differences in HbA1c levels between two groups. The average HbA1c in female Vegan group was higher than in control group (5.9 ±0.9 so với 4.3 ± 0.90%, p < 0.05). The prevalence of hyperglycemia (based on HbA1c) in vegan group was higher than in control group (45.1% vs 13.2%, p < 0.05); prediabetes was 34% in Vegan group and 10.3% in control group. There were correlations between duration of vegan diet and FG (r = 0.312), HbA1c (r = 0.403), in which the duration of vegan diet was considered as an independent risk factor for hyperglycemia. Prediabetes was 18 yrs and diabetes was 42 yrs of vegan diet duration. Conclusions: Vegan diets in women can predict hyperglycemia after an 18-year period. Key words: duration of vegan diet, hyperglycemia, HbA1c

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