scholarly journals Biocontrol of solid surfaces in hospitals using microbial-based wipes

2017 ◽  
Vol 89 (2) ◽  
pp. 216-222 ◽  
Author(s):  
Aysin Dural-Erem ◽  
Per Wessman ◽  
Ulrika Husmark ◽  
Vincent Nierstrasz
Keyword(s):  
Physical Properties ◽  
Hospitalized Patients ◽  
Solid Surfaces ◽  
Cross Contamination ◽  
Beneficial Bacteria ◽  
Nosocomial Pathogens ◽  
Hospital Acquired Infections ◽  
Hospital Acquired ◽  
Number Of Bacteria

Hospital-acquired infections have become a major challenge which threaten the hospitalized patients’ safety. The presence of nosocomial pathogens is generally reported in connection with solid surfaces near patient environments. These surfaces become significant sources of transmission and lead most often to the contamination and cross-contamination of nosocomial pathogens to the patients and staff. This paper investigates strategies to apply beneficial bacteria on viscose-based nonwoven wipes and the viability of these beneficial bacteria on the wipes along with characterization of the physical properties of the wipes. Major findings include that it is possible to produce dry wipes which contain an adequate number of beneficial bacteria or spores. After these wipes are wetted, they can release a certain number of bacteria from the wetted wipes. These released beneficial bacteria can inhibit pathogens by growing and colonizing on the wiped surfaces.

scholarly journals Characterization of the Enterobacter Phage vB_EclM_CIP9

2020 ◽  
Vol 9 (13) ◽  
Author(s):  
Klara Wang ◽  
Marielou G. Tamayo ◽  
Tiffany V. Penner ◽  
Bradley W. M. Cook ◽  
Deborah A. Court ◽  
...  
Keyword(s):  
Enterobacter Cloacae ◽  
Opportunistic Pathogen ◽  
Multidrug Resistant ◽  
Open Reading Frames ◽  
Resistant Isolate ◽  
Content Type ◽  
Hospital Acquired Infections ◽  
Hospital Acquired ◽  
Reading Frames

Enterobacter cloacae is an opportunistic pathogen that causes hospital-acquired infections in immunocompromised patients. Here, we describe vB_EclM_CIP9, a novel Enterobacter phage that infects a multidrug-resistant isolate of E. cloacae. Phage vB_EclM_CIP9 is a myovirus that has a 174,924-bp genome, with 296 predicted open reading frames.

scholarly journals Analytical approach for a better control of environmental contamination

2020 ◽  
Vol 30 (Supplement_5) ◽  
Author(s):  
R Bosco ◽  
S Gambelli ◽  
V Urbano ◽  
G Cevenini ◽  
G Messina
Keyword(s):  
Analytical Approach ◽  
Cross Sectional Study ◽  
Wilcoxon Test ◽  
Lower Probability ◽  
Contamination Level ◽  
Cross Contamination ◽  
Cross Sectional ◽  
Hospital Acquired Infections ◽  
Hospital Acquired ◽  
Selection Of

Abstract Background The environment of hospital rooms plays a role on Hospital Acquired Infections. Objects may be repository of germs and have a role on cross contamination, mainly in high-touch surfaces. Proper sanitation and disinfection procedures are needed. Aims i) to highlight the different probabilities of microbial contaminations in Hospital, ii) to assess whether the use of a UVC device (UVC-D), after standard disinfection procedures, improves the level of hygiene. Methods Between November 2019 and February 2020 a cross sectional study in a real clinical context was conducted. Investigations were carried out in double rehabilitation rooms with patients admitted for at least 48h. 16 preliminary Petri dishes (PD) were used in one rehabilitation room and bathroom, to assess the contamination level after deep disinfection procedures (T0). Matched comparisons were made after the use of UVC-D, 3 points/room and 1 point/bathroom, 3 minutes each(T1). At T0 we estimated the probability of contamination to select the following sampling. Six rooms and bathrooms were investigated using randomized spots. PD were incubated at 36 °C and colony forming unit (CFU) counted at 48h. Descriptive statistic and Wilcoxon test were performed to assess the levels of contamination between (T0) and (T1). Results At T0 we have a mean of 5.83 CFU/PD (CI 3.79-7.86) and a median of 1.0 CFU/PD (min 0 - max 118); at T1 mean is 0.28 CFU/PD (CI 0.15-0.40) and median of 0 CFU/PD (min 0-max 5). Significant reduction of contamination was achieved between T0 and T1 (p < 0.001). 83,33% of the contamination sites had 0 CFU/PD after using UVC-D. Conclusions The probability approach has driven the selection of the highest contaminations spots. Standard disinfection, followed by using UVC-D, significantly reduces contamination assuring a lower probability of cross contamination and risk of infection. Key messages Analytical approach to assess the contamination level of room to address with greater precision the points with higher risk of cross contamination. The UVC technology assures better results when associated with standard sanitation procedure decreasing the risk of hospital acquired infections.

scholarly journals Bacteriophages can be used as infection control agents: A proof-of concept study involving anti-Acinetobacter baumannii phage

2019 ◽  
Author(s):  
Aamir Hussain ◽  
Amna Manzoor ◽  
Ihsan Ullah ◽  
Atif Aziz ◽  
Mubashar Aziz ◽  
...  
Keyword(s):  
Infection Control ◽  
Acinetobacter Baumannii ◽  
Positive Control ◽  
Solid Surfaces ◽  
Proof Of Concept ◽  
Environment Friendly ◽  
Hospital Acquired Infections ◽  
Extensively Drug Resistant ◽  
Acinetobacter Spp ◽  
Hospital Acquired

AbstractHospital acquired infections are responsible for morbidity and mortality worldwide. Acinetobacter spp. infections are particularly notorious for complicating patient management in ICU settings. Extremely high mortality rates are associated with Acinetobacter infections because of their resistance to first- and second-line drugs. There is imminent need to develop infections control systems that are specific and environment friendly. Here, we report a proof-of-concept anti-Acinetobacter spp. bacteriophage-based infection control assay that is very target specific as well as innocuous to environment. extensively drug resistant (XDR) Acinetobacter baumannii strain was inoculated at various solid surfaces. A bacteriophage, enriched in the same strain, was applied on the inoculated surfaces. Phenol (carbolic acid) was used as a positive control. We show that bacteriophages can be used as infection control agents. In our assay, they killed XDR Acinetobacter baumannii present on solid surfaces. Our bacteriophage was extremely effective at reducing the CFU of inoculated strain to almost undetectable levels.

scholarly journals A Nurse-Led Low-Cost Intervention Effectively Traces Prevalence of Catheter Associated Urinary Tract Infections at a Low-Resourced Regional Referral Hospital in Western Uganda: A Case for Policy Change

2019 ◽  
Vol 21 (1) ◽  
pp. 4-11
Author(s):  
Charles Etyang ◽  
Grace Nambozi ◽  
Laura Brennaman
Keyword(s):  
Health Care ◽  
Urinary Tract ◽  
Health System ◽  
Low Cost ◽  
Hospitalized Patients ◽  
Resource Constrained ◽  
Hospital Acquired Infections ◽  
The Government ◽  
Tract Infections ◽  
Hospital Acquired

Catheter associated urinary tract infection (CAUTI) is the most common hospital-acquired infection worldwide. Low- and middle-income countries (LMICs) with limited resources for health care have not allocated resources to adequately monitor or prevent CAUTIs. The infection is associated with several adverse clinical outcomes, including antibiotic resistance, septicemia, and prolonged hospital stays, that burden the already resource-constrained health systems in LMICs with increased morbidity, health care costs, and deaths. Owing to the lack of resource allocation, little is known about the prevalence of CAUTI in the government-owned and operated hospitals in LIMCs. The purpose of this research was to test a method of CAUTI prevalence surveillance suitable to the resource-constrained health system in a LMIC and to determine the prevalence of CAUTI among hospitalized patients at the study site. In an intermittent 4-week data collection plan, the sample of 68 catheterized adult participants was evaluated for the presence of CAUTI using the three-pronged screening criteria of American Urological Society. CAUTI prevalence in the sample was 17.6%. The high prevalence of CAUTI in this sample represents a substantial risk of consequences to hospitalized patients and to the resource-constrained health system in this LMIC. This first report of CAUTI surveillance using readily available and affordable tools provides evidence to health ministry policymakers of the need for and value of monitoring and prevention programs for hospital-acquired infections in LMICs. We recommend LMIC health policymakers to establish infection prevention teams in hospitals and provide resources to continue surveillance and prevention of CAUTI and other hospital-acquired infections.

Characterization of 2 Klebsiella pneumoniae carbapenemase–producing Enterobacterales isolated from canine rectal swabs

2021 ◽  
pp. 104063872110655
Author(s):  
Stephen D. Cole ◽  
Shelley C. Rankin
Keyword(s):  
Klebsiella Pneumoniae ◽  
Companion Animals ◽  
E Coli ◽  
Hospital Acquired Infections ◽  
Klebsiella Pneumoniae Carbapenemase ◽  
Rectal Swabs ◽  
Life Threatening ◽  
Hospital Acquired ◽  
Yorkshire Terrier

Globally, carbapenemase-producing Enterobacterales (CPE) cause life-threatening, hospital-acquired infections in people, and have been reported recently among veterinary patients. Organisms that produce a Klebsiella pneumoniae carbapenemase (KPC) are one of the most common CPE isolated from people but have been reported only rarely in animals. We characterized 2 KPC-producing Enterobacterales isolated from companion animal rectal swabs during the response to an outbreak caused by a strain of blaNDM-5 Escherichia coli. Both isolates were characterized by whole-genome sequencing (WGS) and analysis. The first isolate (case A) was from an immunosuppressed 6-y-old Yorkshire Terrier and was identified as E. coli (ST372) with a blaKPC-18 gene and an IncFII plasmid. The second isolate (case B) was from a 3-y-old Labrador Retriever with acute diarrhea and was identified as Citrobacter koseri with a blaKPC-2 gene, multiple plasmids (ColRNAI, pKPC-CAV1193), and a putative enterotoxin gene ( senB). Further research is needed to determine what role animals might play in the epidemiology of CPE in communities. It is imperative that all CPE isolated from companion animals be fully characterized by WGS and the associated case examined. All veterinary isolates should be sequenced and shared for surveillance, monitoring, and investigation purposes.

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