Adherence of Blood Cells to Dialyzer Membranes as a Measure of Biocompatibility

1991 ◽  
Vol 14 (11) ◽  
pp. 698-702 ◽  
Author(s):  
P. Kjellstrand ◽  
P. Okmark ◽  
R. Odselius ◽  
H. Thysell ◽  
G. Riede ◽  
...  
Keyword(s):  
Membrane Surface ◽  
Surface Chemical ◽  
Cell Adherence ◽  
Polycarbonate Membrane ◽  
Flow Geometry ◽  
Chemical Factors ◽  
Mechanical Factors ◽  
Membrane Type ◽  
Suitable Measure ◽  
Main Factor

Cell adherence to plate dialyzer membranes was analysed at the end of 4 hours of dialysis. Three types of membranes were examined: Cuprophan® Hemophan® and Gambrane®, (a polycarbonate membrane). The membranes were mounted in dialyzers that contained 23 layers of one membrane type and one layer of each of the two other. Less leukocytes adhered to the Pc than to the Cu and He membranes. Transient initial complement activation during dialysis, which was considerably lower with dialysers containing mainly Pc membrane, was not correlated to adherence of cells to the membranes. Instead flow geometry is proposed as the main factor determining the adherence. Contrary to what has been earlier suggested, we think that leukocyte adherence is not a very suitable measure of membrane biocompatibility. The reason is that the influence of membrane surface-chemical factors can not be separated from mechanical factors due to the design of the device.

Influence of Membrane Structure and Chemical Characteristics on Separation and Fouling of Nanofiltration Membranes

2013 ◽  
Vol 864-867 ◽  
pp. 394-398
Author(s):  
Li Qing Zhang ◽  
Gang Zhang
Keyword(s):  
Surface Morphology ◽  
Membrane Fouling ◽  
Membrane Structure ◽  
Membrane Surface ◽  
Chemical Characteristics ◽  
Surface Chemical ◽  
Nanofiltration Membranes ◽  
Factors Affecting ◽  
Comprehensive Literature Review ◽  
Physical Chemical

Nanofiltration membranes act an important role in the advanced water treatment as well as waste water reclamation and other industrial separations. Therefore, an understanding of the factors affecting NF separation and membrane fouling in high-pressure membrane systems is needed. Recent studies have shown that membrane surface morphology and structure as well as surface chemical characteristics influence permeability, rejection, and fouling behavior of nanofiltration (NF) membranes. A comprehensive literature review is reported, targeting the physical-chemical characteristics of NF membrane affecting separation and fouling, including pore size, porosity, surface morphology (measured as roughness), surface charge, and hydrophobicity/ hydrophilicity.

scholarly journals Membrane interactions with membrane type 1 matrix metalloproteinase

2018 ◽  
Author(s):  
◽  
Tara Marcink
Keyword(s):  
Cell Surface ◽  
Matrix Metalloproteinase ◽  
Catalytic Domain ◽  
Membrane Surface ◽  
Membrane Binding ◽  
Tumor Cell Migration ◽  
Peripheral Protein ◽  
Membrane Type ◽  
Dynamics Simulations

Membrane type 1 matrix metalloproteinase (MT1-MMP) is essential to a myriad of extracellular activities including tumor cell migration and angiogenesis. At the cell surface, MT1-MMP is a major factor in the proteolysis of receptors, growth factors, and collagen. MT1-MMP extracellular domains bind the cell surface which can be influential in bringing these complexes together. This study uses new techniques to uncover the interactions between MT1-MMP and the cell surface. Described here is the development of techniques in protein and lipid preparations, NMR data acquisition, and structure determination by molecular dynamics simulations. Through these methods, the HPX domain was shown to bind nanodiscs by opposing tips of blade II and blade IV. The protruding part of these tips contain an EPGYPK sequence that are seen dipping into the membrane surface making contact with the lipid head groups. Blade IV membrane binding allows collagen to bind unhindered. Both blade II and blade IV membrane binding structures are shown to be favorable for homodimerization without disruption of the collagen binding site. The catalytic domain is shown to at least transiently bind membranes. This study then hypothesizes and discusses how these interactions impact both future peripheral protein membrane interaction studies and uncover similarities between the MMP family.

scholarly journals MOLECULAR-GENETIC MECHANISMS OF PLASMODIUM FALCIPARUM VIRULENCE AND TROPICAL MALARIA PATHOGENESIS

2018 ◽  
Vol 10 (3) ◽  
pp. 23-29
Author(s):  
A. N. Uskov ◽  
A. I. Soloviev ◽  
V. Yu. Kravtsov ◽  
R. V. Gudkov ◽  
E. V. Kolomoets ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Membrane Surface ◽  
Complex Structure ◽  
Molecular Genetic ◽  
Regulation Of Gene Expression ◽  
Clinical Malaria ◽  
Tissue Level ◽  
Cellular Level ◽  
Genetic Mechanisms ◽  
Main Factor

There is introduced the analysis of molecular-genetic mechanisms of tropical malaria pathogenesis and P. falciparum virulence. It is shown, that pathogenesis of tropical malaria is associated with the properties of red blood cells membrane surface (RBCs or erythrocytes) that are infected by P. falciparum. There are «knobs structures» on membrane surface infected RBCs. Knobs structures contains a complex of P. falciparum proteins – PfEMP1 (Plasmodium falciparum erythrocyte membrane protein 1). PfEMP1 is associated with virulence of P. falciparum. Complex PfEMP1 has difficult polymorphous structure. Domains of PfEMP1 are able to associate with different cell receptors. Virulence`s individual components of the main factor are selectively sensitive to different tissues and organs. The severity of the clinical malaria infection course depends on the complex structure PfEMP1 of malaria parasites. Composition of polypeptide PfEMP1 is determined by var-complex. Nowadays there are 60 variants of var-complex. Regulation of gene expression, forming part of the var-complex, is carried out on a molecular-genetic level, cellular level, tissue level. Modern research in this area are aimed to explore genes polymorphism of the virulence`s main factor, to identify mechanism of its differential expression. Search of molecular – genetic markers is relevant to develop methods of gene diagnostic and malaria vaccine.

Improving the Surface Wettability of Parylene C Film by Hyperthermal Hydrogen Induced Cross-Linking Technology

2015 ◽  
Vol 1120-1121 ◽  
pp. 64-67
Author(s):  
Hong Shao ◽  
Chang Yu Tang ◽  
Mao Bing Shuai ◽  
Ke Qing Xu ◽  
Yuan Lin Zhou ◽  
...  
Keyword(s):  
Contact Angle ◽  
Water Contact Angle ◽  
Membrane Surface ◽  
Surface Wettability ◽  
Cross Linking ◽  
Surface Chemical ◽  
Water Contact ◽  
Parylene C ◽  
Wetting Property ◽  
Polar Functional Groups

In this paper, we present a new approachcalled hyperthermal hydrogen induced cross-linking (HHIC) technology,which can successfully enhance the surface wettability of Parylene C (PC) film via cross-linking hydrophilic polyacrylic acid（PAA）on its surface. Water Contact Angle Meter and Fourier Transform Infrared Spectroscopy (FTIR) were used to characterize wetting property, surface chemical structureof PC film before or after HHIC treatment,respectively. The results show that PAA with polar functional groups can be successfully grafted onto the PC film. As a result, the water contact angle of the modified PC membrane surface decreased from 84° to 23°,PC membrane surface wettability is improved effectively.

Keterkaitan Status Gizi Dengan Kejadian Phlebitis Pada Pasien Dewasa Di Ruang Flamboyan RSUD Gambiran Kota Kediri

2017 ◽  
Vol 3 (1) ◽  
pp. 48
Author(s):  
Budi Hartanto ◽  
Moh Alimansur
Keyword(s):  
Sampling Method ◽  
Sampling Technique ◽  
Simple Random Sampling ◽  
Adult Patients ◽  
Test Results ◽  
Vein Wall ◽  
Regression Test ◽  
Chemical Factors ◽  
Mechanical Factors ◽  
Logistic Regression Test

Phlebitis is inflammation of the vein wall due to intravenous fluid therapy, which is characterized by pain, redness, palpable soft, swollen and warm to the location of the stabbing (Prastika, 2011). Plebitis as one form of nosocomial infection is still a serious problem faced by hospitals throughout the world, especially hospitals in developing countries (Ministry of Health, 2008). The study design used by researchers is the correlation, this surveys or research that attempts to explore how and why the health phenomena occur. The population in this study are all adult patients in hospitals Gambiran Flamboyan Room Kediri in May. Sampling technique, which is used in this study is the probability sampling which simple random sampling method. Data already collected was processed and identified, then analyzed analytically using logistic regression test. Results of the study appear no link nutritional status in adult patients with phlebitis incidence (p=0.604; 0.05). The risk of the occurrence of phlebitis is caused by chemical factors, mechanical factors and infection.; Keywords: Nutrition, Plebitis, infection

The Characteristics of Frictional Behaviour in CMP Using an Integrated Monitoring System

2007 ◽  
Vol 339 ◽  
pp. 152-157 ◽  
Author(s):  
Hyun Seop Lee ◽  
Boum Young Park ◽  
Sung Min Park ◽  
Hyoung Jae Kim ◽  
Hae Do Jeong
Keyword(s):  
Monitoring System ◽  
Friction Force ◽  
Removal Rate ◽  
Thermal Characteristics ◽  
Integrated Monitoring ◽  
Infrared Rays ◽  
Force Monitoring ◽  
Chemical Factors ◽  
Efficient Prediction ◽  
Mechanical Factors

Chemical mechanical polishing (CMP) has become the preferred technology to achieve global planarization of wafer surfaces. Especially in oxide CMP, mechanical factors have a greater effect on the removal rate than chemical factors. So, the effects of mechanical parameters in CMP can be expressed as friction force and heat caused by friction. The friction force can be evaluated by a CMP friction force monitoring system and process temperature can be obtained by an infrared rays (IR) sensor. Furthermore, friction energy can be calculated from the friction force monitoring system. In this paper, research on the correlation between frictional and thermal characteristics of SiO2 slurry and CMP results was conducted. This data, which was obtained by using integrated monitoring system for CMP, will lead to the efficient prediction of CMP results.

scholarly journals Incidence of Phlebitis Following the Use of Peripheral IV Line at X Hospital

2021 ◽  
pp. 79-86
Author(s):  
Margareta Sijabat ◽  
Sisilia Desiana Nduru ◽  
Ayu Monaretha B ◽  
Yenni Ferawati Sitanggang ◽  
Elissa Oktoviani Hutasoit
Keyword(s):  
Medication Administration ◽  
Infusion Therapy ◽  
Inclusion Criteria ◽  
Intrinsic Factors ◽  
Line Therapy ◽  
Modifiable Factors ◽  
Chemical Factors ◽  
Mechanical Factors ◽  
Infusion Fluid ◽  
Catheter Location

Introduction: Intravenous (IV) line infusion therapy is a therapy given to patients who are admitted or having a specific therapy. The IV-line therapy may include fluid therapy, medication administration and blood therapy. Based on the data found in Hospital X, there were 30 incidence of phlebitis in 2017. This study aimed to describe the factors of phlebitis incidence in X hospital. Method: This study was a retrospective study using 50 clinical record of the inpatient patients. The inclusion criteria were all documents of those who are hospitalized at least three days. Result: The result describes three factors following the incidence of phlebitis, such as intrinsic factors, chemical factors, and mechanical factors. The Intrinsic factors included age, gender and medical diagnosis. While Mechanical factors consist of the size of catheter, location and length of infusion. The Chemical factors were of infusion fluid type and infusion rate. Conclusion: The conclusion of this study was the three factors are modifiable factors. Thus, nurses need to assess and evaluate patients’ infusion in order to prevent a higher case of phlebitis. As a recommendation for further study is to analyze the correlation between those factors to the incidence of phlebitis. 

Role of spectrin in membrane fusion and in shape change of human erythrocyte ghost

1978 ◽  
Vol 36 (2) ◽  
pp. 328-329
Author(s):  
Hideo Hayashi ◽  
Yoshikazu Hirai ◽  
John T. Penniston
Keyword(s):  
Conformational Changes ◽  
Human Erythrocyte ◽  
Shape Change ◽  
Membrane Surface ◽  
Slight Modification ◽  
Active Role ◽  
Main Role ◽  
Bank Blood ◽  
Human Erythrocyte Ghost

Spectrin is a membrane associated protein most of which properties have been tentatively elucidated. A main role of the protein has been assumed to give a supporting structure to inside of the membrane. As reported previously, however, the isolated spectrin molecule underwent self assemble to form such as fibrous, meshwork, dispersed or aggregated arrangements depending upon the buffer suspended and was suggested to play an active role in the membrane conformational changes. In this study, the role of spectrin and actin was examined in terms of the molecular arrangements on the erythrocyte membrane surface with correlation to the functional states of the ghosts.Human erythrocyte ghosts were prepared from either freshly drawn or stocked bank blood by the method of Dodge et al with a slight modification as described before. Anti-spectrin antibody was raised against rabbit by injection of purified spectrin and partially purified.

Role of PKC isozymes in water transport in toad urinary bladder

1991 ◽  
Vol 49 ◽  
pp. 302-303
Author(s):  
A.J. Mia ◽  
L.X. Oakford ◽  
T. Yorio
Keyword(s):  
Urinary Bladder ◽  
Apical Membrane ◽  
Membrane Surface ◽  
Protein A ◽  
Cell Types ◽  
Leukemic Cells ◽  
Toad Urinary Bladder ◽  
Pkc Isozymes ◽  
Antibody Labeling

Protein kinase C (PKC) isozymes, when activated, are translocated to particulate membrane fractions for transport to the apical membrane surface in a variety of cell types. Evidence of PKC translocation was demonstrated in human megakaryoblastic leukemic cells, and in cardiac myocytes and fibroblasts, using FTTC immunofluorescent antibody labeling techniques. Recently, we reported immunogold localizations of PKC subtypes I and II in toad urinary bladder epithelia, following 60 min stimulation with Mezerein (MZ), a PKC activator, or antidiuretic hormone (ADH). Localization of isozyme subtypes I and n was carried out in separate grids using specific monoclonal antibodies with subsequent labeling with 20nm protein A-gold probes. Each PKC subtype was found to be distributed singularly and in discrete isolated patches in the cytosol as well as in the apical membrane domains. To determine if the PKC isozymes co-localized within the cell, a double immunogold labeling technique using single grids was utilized.

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