scholarly journals Identification of bioactive compounds in ethylacetate fraction of Tapinanthus bangwensis leaves that ameliorate CCl4-induced hepatotoxicity in Wistar rats

2020 ◽  
Vol 4 ◽  
pp. 239784732093150
Author(s):  
Godwin Okwudiri Ihegboro ◽  
Adamu Jibrin Alhassan ◽  
Chimaobi James Ononamadu ◽  
Mohamed Sani Sule
Keyword(s):  
Antioxidant Activity ◽  
Wistar Rats ◽  
Chemical Compounds ◽  
Positive Control ◽  
Positive Control Group ◽  
Control Group ◽  
Protein Levels ◽  
Ms Analysis ◽  
Normal Hepatocyte ◽  
Hepatotoxic Effect

The pharmacological effects of medicinal plants are due to the presence of certain chemical compounds present in them. Previous studies have shown that crude extracts of Tapinanthus bangwensis ( T. bangwensis) possess hepatocurative potential. However, the present study aims on evaluating the antioxidant activity as well as identifying the chemical compounds in ethylacetate fraction of T. bangwensis that ameliorate carbon tetrachloride-induced hepatotoxicity in Wistar rats. Six subfractions of ethylacetate fraction were obtained by column chromatography. The antioxidant activity was determined by 2,2-diphenyl-1-picrylhydrazine (DPPH), biochemical assay determined by spectrophotometry, and compound elucidation by liquid chromatography–mass spectroscopy (LC-MS) analysis. The DPPH result shows that subfraction F0 exhibited the strongest antioxidant activity followed by F4 while F1 exhibited the lowest. Oral administration of 100 mg/kg body weight of the subfractions of ethylacetate fraction of T. bangwensis increases superoxide dismutase and catalase activities and also glutathione level and decreases malondialdehyde level compared to the positive control group. Subsequently, aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, total bilirubin, and conjugated bilirubin were reduced while albumin and total protein levels increased compared to the positive control group. However, there were no significant differences between the positive control group and the group induced and treated with the subfractions at p < 0.05. The histopathology study shows normal hepatocyte distribution with no fat deposit in the induced and treated groups while fatty liver was observed in the positive group. The anti-hepatotoxic effect was higher in F4 than in other subfraction treated groups. Hence, the LC-MS analysis of F4 reveals the presence of 8-hydroxyluteolin-8-glucoside, Avicularin, Fisetin-7-glucoside, Isoscutellarein-7-xyloside, and Isovitexin, respectively, and has been reported to exhibit antioxidant and hepatocurative activities. It can be concluded that the hepatocurative effect could be due to the chemical compounds identified above.

scholarly journals ANTIOXIDANT AND HEPATOPROTECTIVE EFFECT OF QUERCUS ILEX LEAVES EXTRACT IN ETHANOL INDUCED LIVER DAMAGE IN WISTAR RATS

2020 ◽  
pp. 26-31
Author(s):  
RENU MALIK ◽  
K. G. SINGHAL
Keyword(s):  
Body Weight ◽  
Wistar Rats ◽  
Quercus Ilex ◽  
Chemical Constituents ◽  
Liver Weight ◽  
Positive Control ◽  
Positive Control Group ◽  
Ethanol Administration ◽  
Control Group ◽  
Standard Drug

Objective: This study was undertaken to investigate the hepatoprotective and antioxidant activity of Quercus ilex leaves extract (QILE) on ethanol-induced toxicity in Wistar rats. Methods: Hepatotoxicity was induced by administering ethanol (40%) at a dose of 7.9 gm/kg/day; p. o. (1:1 of ethanol in olive oil) for 28 d. Silymarin 100 mg/kg/day; p. o. was used as a standard drug. The whole study was divided into a prophylactic and curative study. In the prophylactic study, the Silymarin and QILE (test drug) 100, 200, and 400 mg/kg Body Weight(BW) given orally one hour before administration of 40% ethanol administration for 28 d. In the curative study, 7 d of treatment of Silymarin and QILE 200 and 400 mg/kg BW was given orally after 28 d of ethanol administration to different groups. Results: Hepatoprotectivity was confirmed by the highly significantly (p<0.001) restoration of elevated biochemical parameters like SGPT, SGOT, ALP, TB, and highly significantly (p<0.001) depleted Albumin and Total protein levels by 200 mg/kg BW QILE in comparison to the positive control group. QILE 200 mg/kg highly significantly (p<0.001) raised the antioxidants by draining the elevated oxidative stress markers in comparison of positive control group. At dose levels QILE 200 mg/kg, significant (p<0.05) protection from loss in body weight and in liver weight was found when the comparison was done with the positive control group. Histopathology revealed that QILE 200 mg/kg reduced the markers of cell necrosis. Conclusion: Present study revealed that Quercus ilex leaves have antioxidant and hepatoprotective activity due to its chemical constituents.

scholarly journals EFFECT OF RED MULBERRY JUICE (MORUS RUBRA) ON THE INFLAMMATORY RESPONSE IN MALE WISTAR RATS DUE TO EXPOSURE TO CIGARETTE SMOKE

2021 ◽  
Vol 16 (3) ◽  
pp. 233
Author(s):  
Rivan Virlando Suryadinata ◽  
Merryana Adriani ◽  
Stefani Cornelia Sardjono
Keyword(s):  
Inflammatory Response ◽  
Cigarette Smoke ◽  
Wistar Rats ◽  
Positive Control ◽  
The Body ◽  
Positive Control Group ◽  
Control Group ◽  
C Reactive Protein ◽  
Reactive Protein ◽  
Mulberry Juice

The increasing number of tobacco users will directly impact to an increase in the number of passive smokers. Various harmful substances that enter the respiratory tract can cause health problems. The airway will recognize these substances as pathogen and activate immune response accordingly. Inflammatory response becomes one of the effects of body compensation due to an increased pathogen. This effect is indicated by the elevation of C-Reactive Protein as one systemic inflammatory response parameters in the body. This study aimed to determine the efficacy of red mulberry juice to reduce levels of C-Reactive Protein in Wistar rats because exposure to cigarette smoke. This is an experimental study with a post-test control group design. Animals were divided into negative control group, positive control group, and 3 treatment groups with different red mulberry doses. The positive control group showed the highest increase   in C-Reactive Protein levels compared with the red mulberry intake group (p<0,001). In conclusion, the administration of red mulberry juice can reduce inflammatory response in rats because of exposure to cigarette smoke.

scholarly journals PENGARUH PEMBERIAN GEL AIR KELAPA GADING (COCOS CAPITATA) TERHADAP TINGKAT KECEMASAN PADA HIPERSENSITIVITAS DENTIN Studi in vivo terhadap tikus wistar jantan

2016 ◽  
Vol 3 (2) ◽  
pp. 133
Author(s):  
Chariza Hanum Mayvita Iskandar ◽  
Yayun Siti Rochmah ◽  
Arlina Nurhapsari
Keyword(s):  
Treatment Group ◽  
Wistar Rats ◽  
Positive Control ◽  
Anxiety Level ◽  
Positive Control Group ◽  
Control Group ◽  
Dentin Hypersensitivity ◽  
Group I ◽  
Significant Difference

Background: Ivory palm water (Cocos capitata) has a high potassium content. Potassium ion can depolarized excited dental nerve on dentin hypersensitivity. This study was to determine the effect of water gel ivory palm against dentine hypersensitivity by the anxiety level of mice using RGS (Rat Grimace Scale).Method: This research has been through ethical clearance which researchmethod was quasy experimental in vivo. The sample was 16 Wistar rats (Rattus novergicus) male 4-5 months with 4 mice in each group. Group I as a positive control. Group II as the ivory palm water gel 10% treatment group. Group III as the ivory palm water gel 70% treatment group. Group IV as a negative control. Research data analysis used Kruskal Wallis test with p <0.05 then continued by Mann Whitney test.Result: Kruskal Wallis test obtained significant difference between the four study groups (p <0.05). Mann Whitney test results in comparison either between positive control group and ivory palm water gel 10% and 70%, and comparison group of ivory palm water gel 10% and 70% weren’t significantly different.Conclusion: This study conclusion there was no significant difference between ivory palm water gel 10 % and 70 % in reducing anxiety level in male Wistar rats with dentinal hypersensitivity

scholarly journals Tissue injuries of wistar rats treated with hydroalcoholic extract of Sonchus oleraceus L.

2011 ◽  
Vol 47 (3) ◽  
pp. 605-613 ◽  
Author(s):  
Franciele Carla Prichoa ◽  
Silvane Souza Roman ◽  
Vanusa Manfredini
Keyword(s):  
Wistar Rats ◽  
Skin Lesions ◽  
Positive Control ◽  
Negative Control ◽  
Hair Follicles ◽  
Positive Control Group ◽  
Control Group ◽  
Control Groups ◽  
Sonchus Oleraceus ◽  
Experimental Group

The use of plant species is emerging as an important alternative in the treatment of injuries. Therefore, the extract of Sonchus oleraceus 10% was employed in the repair of skin lesions. A total of 36 male Wistar rats were subjected to a punch injury and divided into three groups: a negative control, receiving no treatment, a positive control, treated with Dersani, and the experimental group treated with the extract. The injury was assessed macroscopically and microscopically. Morphometric data was collected at the 3rd, 5th and 7th postoperative day, and the experimental group showed greater changes in shrinkage of the lesion compared to control groups. On the 3rd postoperative day, the injury in the experimental group showed less necrotic tissue, lower slough and more granulation tissue in relation to the positive control group. On the 7th and 10th postoperative day, the injury in the experimental group showed lower slough compared to the positive control group. Microscopic analysis of lesions on the 5th postoperative day revealed increased fibroplasia in the experimental group compared to control groups, while on the 14th postoperative day less neovascularization was evident in the experimental group and increased formation of hair follicles in the negative control group. The extract of S. oleraceus provided tissue repair in accordance with normal physiological patterns thus confirming empirical evidence for its use.

scholarly journals THE EFFECT OF NIFEDIPINE ON SURVIVE CELL, APOPTOTIC CELL AND NECROTIC CELL OF IPSILATERAL TESTICULAR GERMINAL EPITELIAL CELLS ON MALE WISTAR RATS WITH UNILATERAL TESTICULAR TORSION

2019 ◽  
Vol 26 (2) ◽  
Author(s):  
Andrie Rhomdhon Kurniawan ◽  
Lukman Hakim ◽  
Doddy M Soebadi
Keyword(s):  
Treatment Group ◽  
Wistar Rats ◽  
Testicular Torsion ◽  
Intraperitoneal Administration ◽  
Positive Control ◽  
Lower Number ◽  
Positive Control Group ◽  
Control Group ◽  
P Value ◽  
Male Wistar Rats

Objective: This study aimed to compare the number of survival, apoptotic and necrotic cells of ipsilateral testicular germinal epithelial cells in male wistar rats with unilateral testicular torsion between nifedipine given and control groups. Material & Methods: Thirty male wistar rats aged 10-12 weeks were randomly divided into 5 groups, each consisted of 6 rats. The negative control group (KN) underwent a sham procedure and left orchidectomy. Positive control group 4 (KP4) and 10 (KP10) performed left torsio testis 3 x 360 degrees medially for 4 hours and 10 hours respectively, then performed orchidectomy 4 hours after detorsion. The 4-hour (N4) and 10 hours (N10) nifedipine treatment group received the same treatment with positive control, but 30 min before detorsion performed, nifedipine were given intraperitoneal 100μg/kg. Within 1 hour after orchidectomy, cell count was calculated using flow cytometry. Results: It was found that the 4 (N4) and 10 hours (N10) nifedipine treatment group had a higher survival cells and also a lower number of apoptotic and necrotic cells compared to the positive control group. It was found that the 10 hours nifedipine treatment group (N10) had a lower number of apoptotic and necrotic cells compared to the 10 hour positive control group (KP10). The difference was statistically significant with p value <0.05. However, in KP4 and N4 group compared with KP10 and N10 group, higher apoptotic cells was obtained. This was a new phenomenon that needs to be investigated more deeply. Conclusion: Intraperitoneal administration of nifedipine prior to testicular detorsion may reduce the number of apoptotic and necrotic cells of testicular germinal epithelial cell, and may increase the number of survival cells in ipsilateral testes with unilateral testicular torsion.

scholarly journals The effects of shark liver oil on fibroblasts and collagen density in the periodontal ligaments of Wistar rats induced with Porphyromonas gingivalis

2019 ◽  
Vol 52 (4) ◽  
pp. 209
Author(s):  
Dian Mulawarmanti ◽  
Dwi Andriani ◽  
Dian Widya Damaiyanti ◽  
Farizia Putri Khoirunnisa ◽  
Alifati Nita Juliatin
Keyword(s):  
Periodontal Ligament ◽  
Wistar Rats ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Positive Control Group ◽  
Control Group ◽  
Treatment Groups ◽  
Shark Liver Oil ◽  
Collagen Density

Background: Periodontitis is an infection in tooth-supporting tissues caused by a specific microorganism, Porphyromonas Gingivalis (PG), which can trigger collagen destruction. Generally, periodontal therapy employs a combination of mechanical (scaling root planning/SRP) and chemical (antibiotics) remedies, the latter of which can cause bacterial resistance. On the other hand, shark liver oil contains active natural ingredients such as alkylglycerols, squalene, squalamine, and omega-3, which have antibacterial and antioxidant effects. Purpose: This study aims to determine the impact of shark liver oil on fibroblasts and collagen density in the periodontal ligament of Wistar rats induced with PG. Methods: This study represents a laboratory experiment with post-test only control group design. The research subjects consisted of 35 Wistar rats divided into five groups, namely; a negative control group (K-); a positive control group with PG induction (K+); and three treatment groups induced with PG and shark liver oil once a day for seven days at varying doses of 0.2 g/gBB (P1), 0.3 g/gBB (P2), and 0.4 g/gBB (P3). Following treatment, the subjects were euthanized. The number of fibroblasts was then histologically examined with Hematoxylin Eosin (HE). Meanwhile, the collagen density was histologically analyzed with Masson’s Tricrome. Fibroblast cells were observed through a microscope at 400x magnification. Data was statistically analyzed with a one-way ANOVA and post hoc LSD. Collagen Density scoring was then performed. The results were analyzed with a non-parametric Kruskal-Wallis test (p=0.05), and subsequently with a Mann-Whitney U test (p<0.05). Results: The number of fibroblasts in the periodontal ligament areas of each group were 18.6 ± 1.21 for K-; 12 ± 1.26 for K;16.8 ± 1.72 for P1; 17.1 ± 1.94 for P2; and 23.16 ± 2.78 for P3. The results also indicated that there were significant differences between K- with K+ and P3, K+ with P1, P2, and P3, as well as P3 with P1 and P2. However, there was no significant difference between K- and P1 and P2 or P1 and P2. The results showed that collagen density in the negative control group did not significantly decrease compared to that in the positive control group in which PG was induced. Meanwhile, collagen density in all three treatment groups following doses of 0.2 g/gBB, 0.3 g/gBB, and 0.4 g/gBB being administered significantly increased compared to that in the negative control group and the positive control group subjected to PG induction. Conclusion: Shark liver oil can significantly increase fibroblast cells and collagen density in the periodontal ligament of Wistar rats induced with PG.

scholarly journals Resistance to Fracture of Dental Roots Obturated with Different Materials

2015 ◽  
Vol 2015 ◽  
pp. 1-5 ◽  
Author(s):  
Berkan Celikten ◽  
Ceren Feriha Uzuntas ◽  
Kamran Gulsahi
Keyword(s):  
Fracture Resistance ◽  
Testing Machine ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Positive Control Group ◽  
Control Group ◽  
Root Canal Filling ◽  
Filling Materials ◽  
Group 2

The aim of this study was to compare the vertical fracture resistance of roots obturated with different root canal filling materials and sealers. Crowns of 55 extracted mandibular premolar teeth were removed to provide root lengths of 13 mm. Five roots were saved as negative control group (canals unprepared and unfilled). Fifty root canals were instrumented and then five roots were saved as positive control group (canals prepared but unfilled). The remaining 45 roots were randomly divided into three experimental groups (n=15root/group) and obturated with the following procedures: in group 1, glass ionomer-based sealer and cone (ActiV GP obturation system); in group 2, bioceramic sealer and cone (EndoSequence BC obturation system); and in group 3, roots were filled with bioceramic sealer and cone (Smartpaste bio obturation system). All specimens were tested in a universal testing machine for measuring fracture resistance. For each root, the force at the time of fracture was recorded in Newtons. The statistical analysis was performed by using Kruskal-Wallis and post hoc test. There were no significant differences between the three experimental groups. The fracture values of three experimental and negative control groups were significantly higher than the positive control group. Within the limitations of this study, all materials increased the fracture resistance of instrumented roots.

scholarly journals Antidiabetic and antihyperlipidemic activities of Cucumis melo var. momordica fruit extract on experimental animals

2020 ◽  
Vol 6 (1) ◽  
Author(s):  
Arvind Kumar Srivastava ◽  
Alok Mukerjee ◽  
Abhishek Tripathi
Keyword(s):  
Blood Glucose ◽  
Cucumis Melo ◽  
Biological Properties ◽  
Positive Control ◽  
Positive Control Group ◽  
Public Health Issue ◽  
Control Group ◽  
Fruit Extract ◽  
Experimental Animals ◽  
Glucose Levels

Abstract Background Diabetes mellitus is a major public health issue related to the irregular metabolism of carbohydrates, protein, and fat. It occurs due to insufficient insulin production and insulin action. Cucumis melo possesses several biological properties including antioxidant, anti-inflammatory, antibacterial, antihypothyroidism, and antiangiogenic activities. The objective of the present study was to determine the antidiabetic and antihyperlipidemic activities of Cucumis melo var. momordica fruit extract on experimental animals. Result Results show that treatment with C. melo fruit extract and fraction caused a reduction in blood glucose levels. Cucumis melo toluene fraction (CMTF) exhibited a significant (*P < 0.05) reduction of blood glucose level on the 28th day, i.e., 122 mg/dL, in comparison with the positive control group (streptozotocin (STZ)). However, the extract of C. melo showed less significant results in comparison with CMTF. Triglyceride, LDL, and VLDL levels were increased chronically due to STZ and were significantly (*P < 0.05) restored to 84.16, 86.97, and 19.73, respectively, by CMTF in comparison with the positive control group (STZ in the dose of 55 mg/kg). The extract-treated groups also showed similar results as CMTF, but their efficacy was lesser than CMTF. Conclusion It is can be concluded that C. melo fruits can be used as an effective antidiabetic and antihyperlipidemic drug. Graphical abstract

Protective effect of Saccharomyces cerevisiae in Rattus norvegicus Ischemic Stroke Model

2021 ◽  
pp. 5785-5789
Author(s):  
Dedy Budi Kurniawan ◽  
Mokhamad Fahmi Rizki Syaban ◽  
Arinal Mufidah ◽  
Muhammad Unzila Rafsi Zulfikri ◽  
Wibi Riawan
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Ischemic Stroke ◽  
Brain Tissue ◽  
Tissue Injury ◽  
Intervention Group ◽  
Positive Control ◽  
Positive Control Group ◽  
Control Group ◽  
Hematopoietic Stem ◽  
The Brain

Stroke is one of the leading causes of morbidity and mortality in all ages. Ischemic stroke activates excitotoxic glutamate cascade leading to brain tissue injury. Saccharomyces cerevisiae is a unicellular yeast widely found in nature. S. cerevisiae is neuroprotective and able to increase the differentiation of hematopoietic stem cells (HSCs) into neuronal cells. it may increase levels of neuroprotectant BDNF in the brain tissue, therefore increase the protection of neurons. BDNF may prevent glutamate-driven excitotoxicity by reducing glutamate levels. This study uses a randomized post-test only controlled group design. In this in vivo study, rodent models of ischemic stroke were divided into five groups comprising of the negative control group, positive control group, intervention group 1 (18mg/kgBW), intervention group 2 (36mg/kgBW) and intervention group 3 (72 mg/kgBW). Groups treated with Saccharomyces cerevisiae extract showed significantly increased BDNF levels in the brain tissue, and the expression of the glutamate level was significantly reduced (P <0.05) compared to the positive control group. Thus Saccharomyces cerevisiae has a promising potential to become a therapy against ischemic stroke disease. however further research is needed regarding the efficacy and toxicity of Saccharomyces cerevisiae.

Effect of Chayote (Sechium Edule Jacq. Swartz) Extract on Level of Interleukin-8 in Wistar Rats with Aspirin-Induced Gastritis

2020 ◽  
Author(s):  
Hendrika Andriana Silitonga ◽  
◽  
Gontar Alamsyah Siregar ◽  
Rosita Juwita Sembiring ◽  
Marline Nainggolan ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Wistar Rats ◽  
Ethanol Extract ◽  
Ethyl Acetate Fraction ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Interleukin 8 ◽  
Control Group ◽  
Sechium Edule

ABSTRACT Background: Recent studies showed that Interleukin-8 (IL-8), activated cytokine immune response which plays an important role in the development of acute and chronic gastritis. Harmless anti-inflammatory therapeutic alternatives have been proposed, for example, the consumption of Sechium Edule Jacq. Swartz (chayote). Antioxidant (flavonoid) and cell regeneration (alkaloid) agents were found in chayote. This study aimed to determine the effect of chayote Sechium Edule Jacq. Swartz extracts on the level of IL-8 in Wistar rats with aspirin- induced gastritis. Subjects and Method: This was a randomized controlled trial (RCT) conducted at the laboratory of Mathematics and Natural Science, Universitas Sumatra Utara from January to February 2020. A total of 35 male Wistar rats was selected for this study and randomly allocated into 7 groups: (1) Negative control; (2) Positive control; (3) 100 mg/ kg BW chayote ethanol extract ; (4) 200 mg/kg BW chayote ethanol extract; (5) 100 mg/ kg BW chayote ethyl acetate fraction; (6) 200 mg/kg BW chayote ethyl acetate fraction; and (7) 20 mg omeprazole. The rats in positive control and treatment groups were induced with aspirin (200mg/ kg BW). The negative control group received no intervention. The dependent variable was level of IL-8 measured by ELISA. The independent variables were treatment status. The data were analyzed by One Way Anova and post hoc test. Results: The mean differences of IL-8 level were not statistically significant between study groups (p= 0.327). Mean of IL-8 level was higher in positive control group (Mean= 160.80; SD= 6.90) than in negative control group (Mean= 141.20; SD= 10.98). The lowest IL-8 level was in 100mg/ kg BW chayote ethanol extract group (Mean= 149.94; SD= 40.4), followed by 200mg/ kg BW (Mean= 152.4; SD= 30.73) and 100mg/ kg BW (Mean= 164.60; SD= 25.04) chayote ethyl acetate fraction groups, 20 mg omeprazole group (Mean= 170.60; SD= 21.58), and 200 mg/ kg BW chayote ethanol extract group (Mean= 176.80; SD= 10.98). Conclusion: The low dose (100mg/ kg BW) chayote ethanol extract has the most potential antiinflammation effect on in vitro gastritis with the lowest IL-8 level of all doses of chayote ethanol extract, chayote ethyl acetate fraction, and omeprazole. Keywords: antiinflammation, IL-8, chayote ethanol extract, ethyl acetate fraction, omeprazole, aspirin induced gastritis Correspondence: Hendrika Andriana Silitonga. Department of Histology, Faculty of Medicine, Universitas Methodist Indonesia. Email: [email protected]. Mobile: +6281361430688. DOI: https://doi.org/10.26911/the7thicph.05.35

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