scholarly journals The antibacterial activity, antioxidant activity and selectivity index of leaf extracts of thirteen South African tree species used in ethnoveterinary medicine to treat helminth infections

2014 ◽  
Vol 10 (1) ◽  
pp. 52 ◽  
Author(s):  
Mathew Adamu ◽  
Vinny Naidoo ◽  
Jacobus N Eloff
Keyword(s):  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
South African ◽  
Tree Species ◽  
Selectivity Index ◽  
Ethnoveterinary Medicine ◽  
Leaf Extracts ◽  
Helminth Infections

scholarly journals Karakteristik Kemasan Aktif dari Film Gelatin Ikan dengan Penambahan Ekstrak Daun Jeruju (Acanthus ilicifolius)

2020 ◽  
Vol 15 (1) ◽  
pp. 63
Author(s):  
Rahmi Nurdiani ◽  
Abdul Aziz Jaziri ◽  
Fitri Septa Puspita
Keyword(s):  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Leaf Extract ◽  
Inhibition Zone ◽  
Active Packaging ◽  
Basic Material ◽  
Leaf Extracts ◽  
Bioactive Substances ◽  
Acanthus Ilicifolius

Kemasan aktif memiliki fungsi yang lebih luas dalam menjaga kualitas suatu produk. Salah satu bahan baku kemasan aktif adalah gelatin dengan penambahan senyawa aktif. Penelitian ini dimaksudkan untuk mempelajari karakteristik dan bioaktivitas kemasan aktif dari gelatin ikan ayam-ayam (Abalistes stellaris) dengan penambahan ekstrak daun jeruju (Acanthus ilicifolius) sebesar 0%, 1%, 3%, 5% dan 7%. Kemasan aktif yang dihasilkan diamati karakteristik dan bioaktifitasnya yang meliputi ketebalan, kuat tarik, elongasi, transmisi uap air, kadar air, pH, aktivitas antioksidan dan aktivitas antibakteri. Hasil analisa sidik ragam menunjukkan penambahan ekstrak daun jeruju berpengaruh nyata (p<0,05) terhadap ketebalan, aktivitas antioksidan dan aktivitas antibakteri dari kemasan aktif berbahan gelatin yang dihasilkan, namun tidak berpengaruh nyata (p>0,05) terhadap kuat tarik, elongasi dan transmisi uap air. Kemasan aktif dari gelatin ikan ayam-ayam dengan penambahan ekstrak daun jeruju 7% memiliki ketebalan 143,01 µm, mampu menghambat 65,45% radikal bebas DPPH dan menghasilkan zona hambat bakteri berkisar antara 2,18-2,61 mm. Hasil penelitian ini menunjukkan gelatin yang terbuat dari kulit dan sisik ikan ayam-ayam dengan penambahan ekstrak daun jeruju memiliki potensi untuk dikembangkan menjadi kemasan aktif.  AbstractPackaging has an important function in maintaining the quality of product. Active packaging can be made from gelatin as basic material with addition of bioactive substances. The purpose of this research was to study the characteristics and bioactivity of active packaging made from staryy triggerfish (Abalistes stellaris) gelatin incorporated with 0, 1, 3, 5, and 7% of Acanthus ilicifolius leaf extract. The active pakaging was observed for its charateristics and bioactivity including thickness,  elongation, tensile strength, water vapor transmission, moisture content, pH, antioxidant activity, and antibacterial activity of active packaging. The statistical analyses showed that the addition of A. ilicifolius leaf extracts significantly affected (p<0.05) the thickness, antioxidant activity, and antibacterial activity of active packaging. Active packaging from fish gelatin containing 7% of A. ilicifolius extract with 143.01 µm thickness, was able to inhibit 65.45% DPPH free radical and resulted in 2.18-2.61 mm inhibition zone. It was suggested that starry triggerfish gelatin and A. ilicifolius leaf extract have a potential to be developed as active packaging.

PHYTOCHEMICAL SCREENING, ANTIBACTERIAL, AND ANTIOXIDANT ACTIVITIES OF CASSIA MONTANA HEYNE EX ROTH LEAF EXTRACTS

2021 ◽  
pp. 41-16
Author(s):  
HEMALATHA M ◽  
BHAKSHU L ◽  
YASODAMMA N
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Antibacterial Activity ◽  
Gallic Acid ◽  
Dry Weight ◽  
Diffusion Method ◽  
Klebsiella Pneumonia ◽  
Phytochemical Screening ◽  
Leaf Extracts ◽  
Water Extracts

Objective: The leaf extracts of Cassia montana (Caesalpiniaceae) were being used for leucorrhea and rheumatic pains used by the certain tribal people in Andhra Pradesh. The present studies focused on the antibacterial, antioxidant activity of leaf extracts of C. montana which provide the scientific basis for its folklore applications in various ailments. Methods: Ethyl-alcohol and water extracts of C. montana (leaf) were screened for phytochemicals, antimicrobial activity and antioxidant activity using in vitro methods. The total phenolics were estimated using Folin–Ciocalteu reagent with reference to gallic acid, whereas the antioxidants of the extracts were quantified using ammonium molybdate reduction assay with reference to the ascorbic acid. The antibacterial activity was studied by disk-diffusion method and the radical scavenging assay by DPPH discoloration method with reference to ascorbic acid. Results: The leaf extracts were tested for antibacterial, antioxidants, and phytochemical screening and found the significant phytoconstituents as steroids, lignins, flavonoids, phenols, glycosides, tannins, cardiac glycosides, and reducing sugars in ethanol and aqueous extracts. The tested extracts exhibited significant antibacterial activity. It is also observed that ethanolic extracts are more effective with minimum inhibition concentrations values at 28 and 32 μg/disk against Bacillus subtilis and Staphylococcus aureus followed by Klebsiella pneumonia and Pseudomonas aeruginosa with 80 and 90 μg/disk. Total polyphenols quantified in alcohol and water extracts with reference to the gallic acid is 108 and 267 mg/g dry weight of leaf, respectively. Total antioxidants quantified in alcohol and water extracts with reference to the ascorbic acid is 70 and 85 mg/g dry weight of leaf, respectively. The tested extracts were exhibited strong effect on the discoloration of DPPH indicated the significant scavenging activity. Conclusion: The present study revealed that the tested extracts were exhibited significant anti-bacterial antioxidant activity along with the diversified phytochemicals. Hence, the leaf extracts of C. montana were having potential role in the treatment of ethno botanical health claims.

IN VITRO EVALUATION OF ANTIBACTERIAL AND ANTIOXIDANT PROPERTIES OF AN INDIAN MEDICINAL PLANT: CELASTRUS PANICULATUS WILLD

2020 ◽  
Vol 9 (1) ◽  
Author(s):  
Pradeep K Naik ◽  
Debasmita Dubey ◽  
Priya Cheruvanachari ◽  
Animesh Pattnaik ◽  
Swaraj K Babu
Keyword(s):  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Antioxidant Properties ◽  
Antibacterial Properties ◽  
Resistant Bacteria ◽  
Dilution Method ◽  
Phytochemical Analysis ◽  
Leaf Extracts ◽  
Dpph Method ◽  
Celastrus Paniculatus

Aim: To evaluate the antibacterial efficacy, the presence of different phytoconstituents of the leaf extracts of Indian plant Celastrus paniculatus against 6 antibiotic-resistant bacteria along with the evaluation of their antioxidant potentiality. Methods: The leaf extracts were prepared using 4 solvents, water, methanol, n-butanol, and acetone. The antibacterial property was evaluated using agar well diffusion and micro-broth dilution method. Qualitative phytochemical analysis was performed using standard protocols for each solvent extract to check for the presence of the secondary metabolites and phytochemicals which attributes to the antibacterial effectivity of C. paniculatus. Further, the antioxidant potentiality of all the 4 leaf-solvent-extracts was estimated using the DPPH method. Results: The n-butanol leaf extract exhibited the highest antibacterial and antioxidant activity, followed by acetone, methanol extracts. The aqueous extracts exhibited negligible antibacterial activity. Conclusion: This study validated the antibacterial properties of C. paniculatus leaf extracts against both gram-positive and negative bacteria and the phytochemical analysis revealed the presence of many secondary importance which can be attributed to its therapeutic properties. Hence, this plant can be further used as a complementary or alternative choice of drug for combatting multidrug-resistant bacterial pathogens. Keywords: Antibiotic resistance; Medicinal plants; Celastrus Paniculatus; Antibacterial activity, Phytochemical Analysis; Antioxidant activity

scholarly journals Antioxidant Activity and Antibacterial Effects on Clinical IsolatedStreptococcus suisandStaphylococcus intermediusof Extracts from Several Parts ofCladogynos orientalisand Their Phytochemical Screenings

2015 ◽  
Vol 2015 ◽  
pp. 1-8 ◽  
Author(s):  
Pongtip Sithisarn ◽  
Piyanuch Rojsanga ◽  
Patchima Sithisarn ◽  
Sumet Kongkiatpaiboon
Keyword(s):  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
High Performance ◽  
Total Flavonoid ◽  
Diffusion Method ◽  
Total Phenolic ◽  
Leaf Extracts ◽  
Spectrophotometric Methods ◽  
Staphylococcus Intermedius

Thein vitroantioxidant and antibacterial assays against clinically isolatedStreptococcus suisandStaphylococcus intermediusof the extracts prepared by decoction and ethanolic reflux of different parts of Chettaphangki (Cladogynos orientalisZipp. ex Span), including the leaves, roots, and stems, using 1,1-diphenyl-2-picrylhydrazyl (DPPH) scavenging assay and disc diffusion method were conducted. Quantitative analysis of total phenolic and total flavonoid contents in the extracts using spectrophotometric methods was also performed. Finally, phytochemical screening by thin layer chromatography (TLC) and high performance liquid chromatography (HPLC) was conducted. Leaf ethanolic reflux extract (100 g) contained the highest total phenolic and total flavonoid contents of 7.21 ± 0.28 μg gallic acid equivalent (GAE) and 11.51 ± 2.02 μg rutin equivalent (RE), respectively. Chettaphangki extracts promoted low antioxidant activity with EC50values in the range of 0.27–0.48 mg/mL. Extracts and fractions from the roots and stems of this plant promoted low to intermediate antibacterial activity againstS. intermediuswith the inhibition zones between 7 and 14 mm. The chromatographic data suggested that the leaf extracts ofC. orientaliscontained rutin while the root and stem extracts contained scopoletin and chettaphanin I. Rutin promoted strong antioxidant activity while chettaphanin I showed low antibacterial activity againstStaphylococcus intermedius.

scholarly journals Phenolic Compounds in Extracts of Hibiscus acetosella (Cranberry Hibiscus) and Their Antioxidant and Antibacterial Properties

Molecules ◽  
2020 ◽  
Vol 25 (18) ◽  
pp. 4190 ◽  
Author(s):  
Jae Il Lyu ◽  
Jaihyunk Ryu ◽  
Chang Hyun Jin ◽  
Dong-Gun Kim ◽  
Jung Min Kim ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Phenolic Compounds ◽  
Antibacterial Properties ◽  
Activity Level ◽  
Leaf Extracts ◽  
Abts Assay ◽  
Activity Group ◽  
Zones Of Inhibition ◽  
Low Activity

Hibiscus species are rich in phenolic compounds and have been traditionally used for improving human health through their bioactive activities. The present study investigated the phenolic compounds of leaf extracts from 18 different H. acetosella accessions and evaluated their biofunctional properties, focusing on antioxidant and antibacterial activity. The most abundant phenolic compound in H. acetosella was caffeic acid, with levels ranging from 14.95 to 42.93 mg/100 g. The antioxidant activity measured by the ABTS assay allowed the accessions to be classified into two groups: a high activity group with red leaf varieties (74.71–84.02%) and a relatively low activity group with green leaf varieties (57.47–65.94%). The antioxidant activity was significantly correlated with TAC (0.933), Dp3-Sam (0.932), Dp3-Glu (0.924), and Cy3-Sam (0.913) contents (p < 0.001). The H. acetosella phenolic extracts exhibited antibacterial activity against two bacteria, with zones of inhibition between 12.00 and 13.67 mm (Staphylococcus aureus), and 10.67 and 13.33 mm (Pseudomonas aeruginosa). All accessions exhibited a basal antibacterial activity level (12 mm) against the Gram-positive S. aureus, with PI500758 and PI500764 exhibiting increased antibacterial activity (13.67 mm), but they exhibited a more dynamic antibacterial activity level against the Gram-negative P. aeruginosa.

scholarly journals Antioxidant and Antibacterial Activities of Four Local Medicinal Plants

2020 ◽  
Vol 30 (2) ◽  
pp. 179-187
Author(s):  
Barna Goswami ◽  
Shamoly Akter ◽  
Nemai Chandra Nandi ◽  
Tanjina Akthar Banu ◽  
Shahina Akter ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Medicinal Plants ◽  
Pathogenic Bacteria ◽  
Leaf Extract ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Centella Asiatica ◽  
Leaf Extracts ◽  
Ic50 Value

Potential antioxidant and antibacterial activity of methanolic, chloroformic and n-hexane leaf extracts of four local important medicinal plants like Ocimum americanum, O. basilicum, O. gratissimum and Centella asiatica was investigated. The methanolic leaf extracts of these plant species exhibited the potent DPPH free radical scavenging activity (IC50 value, 2.67 ± 0.01, 14.17 ± 0.11, 60.22 ± 0.01 and 2.39 ± 0.025 μg/ml, respectively). Methanolic leaf extract of C. asiatica showed strongest antioxidant activity. Chloroformic leaf extracts possessed moderate antioxidant activity (IC50 value of 79.44 ± 0.05, 110.56 ± 0.02, 54.95 ± 0.05, 101.0 ± 1.0 μg/ml, respectively) in all samples. The lowest antioxidant activity was recorded from n-hexane leaf extracts of O. americanum, O. gratissimum, C. asiatica and Ocimum basilicum (IC50 value 147.87 ± 0.06, 378.19 ± 2.65, 104.65 ± 0.39, 467.58 ± 0.52 μg/ml, respectively). Methanolic and chloro-formic leaf extracts showed antibacterial activity against both Gram-positive and Gram-negative pathogenic bacteria, namely Bacillus megaterium, Escherichia coli, Pseudomonas aeruginosa and Salmonella typhi. Methanolic leaf extract of O. americanum and chloroformic extract of C. asiatica showed excellent antimicrobial activity. Plant Tissue Cult. & Biotech. 30(2): 179-187, 2020 (December)

scholarly journals Roots and Leaf Extracts of Dipsacus fullonum L. and Their Biological Activities

Plants ◽  
2020 ◽  
Vol 9 (1) ◽  
pp. 78 ◽  
Author(s):  
Jan Oszmiański ◽  
Aneta Wojdyło ◽  
Piotr Juszczyk ◽  
Paulina Nowicka
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Phenolic Compounds ◽  
Phenolic Acids ◽  
Leaf Extract ◽  
Biological Activities ◽  
Leaf Extracts ◽  
Leaves And Roots

The aim of the study was to identify and evaluate the content of iridoids and phenolic compounds in the leaves and roots of Dipsacus fullonum L. They were identified and quantified by UPLC-PDA-MS/MS. Five iridoid compounds (loganic acid, loganin, sweroside, cantleyoside, and sylvestroside III) were identified in Dipsacus fullonum L. leaves and roots. Seven phenolic acids and three flavones were identified in the leaves, and seven phenolic acids were detected in the roots. The leaves contained more iridoids and phenolic compounds than the roots. We also evaluated the antimicrobial (anti-bacterial and anti-yeast), antioxidant (ORAC methods), and antiacetylcholinesterase (AChE) activities of Dipsacus fullonum L. leaves and roots. Leaf extract demonstrated the strongest antioxidant activity, but roots showed stronger antiacetylcholinesterase activity than leaves. The study also confirmed antibacterial activity of root-derived compounds against Staphylococcus aureus DSM 799 and Escherichia coli ATCC 10536.

scholarly journals Antioxidant and Antibacterial Activity of Anacardium occidentale and Psidium guajava Methanolic Leaf Extracts

2020 ◽  
pp. 29-37
Author(s):  
Mutiat Adetayo Omotayo ◽  
Mary Oluwatoyin Avungbeto ◽  
Oluwole Olusoji Eleyowo
Keyword(s):  
Antimicrobial Activity ◽  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Psidium Guajava ◽  
Anacardium Occidentale ◽  
Total Phenolic ◽  
Leaf Extracts ◽  
Absorbing Materials ◽  
Laboratory Technology ◽  
Β Carotene

Aim: This study evaluates the antioxidant and antibacterial activity of Anacardium occidentale and Psidium guajava methanolic leaf extracts. Place and Duration of Study: The study was carried out in the Biochemistry and Microbiology Laboratory, Department of Science Laboratory Technology, School of Pure and Applied Science, Lagos State Polytechnic, Ikorodu, Lagos- Nigeria for the period of three months between August and October 2015. Methodology: Lycophene and β-carotene was assessed using the method of Nagata and Yamashita while total phenolic and total flavonoid content was assessed by the Folin-Ciocalteau assay and aluminum chloride colorimetric assay respectively. The antioxidant activity was evaluated using the DPPH radical scavenging activity. Antimicrobial activity was assessed by the agar well diffusion technique and mode of action was evaluated by studying the leakage of UV260 and UV280 absorbing materials spectrophotometrically. Results: A. occidentale and P. guajava methanolic leaf extracts evaluated in this study possessed significant amount of antioxidant compounds lycophene, β-carotene, total phenol and flavonoids. The extracts exhibited antioxidant activity by scavenging DPPH radicals in a dose dependent pattern with IC50 of 47.45, 43.49, 41.46 and 27.21 μg/mL for A. occidentale, P. guajava, vitamin C and Gallic acid respectively. Also, the plant extracts exhibited antimicrobial activity against E. coli, S. aureus, P. auraginosa and C. albicans and disrupted microbial membrane evident in the increase in absorbance values of UV260 and UV280 absorbing materials with time. Conclusion: A. occidentale and P. guajava methanolic leaf extracts possess antioxidant and antimicrobial activity and serve as potential source of drugs.

scholarly journals Bioactive properties of Persea lingue Ness (Lauraceae) fruit and leaf extracts

2023 ◽  
Vol 83 ◽  
Author(s):  
A. Lillo ◽  
F. Carvajal-Caiconte ◽  
W. Vital ◽  
P. I. Silva Junior ◽  
E. J. Paredes-Gamero ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Cytotoxic Activity ◽  
Cell Lines ◽  
Leaf Extract ◽  
Leukemia Cell ◽  
Total Phenolic ◽  
Leaf Extracts ◽  
Fruit Extract ◽  
South Central

Abstract Persea lingue Ness is a tree species that lives mainly in temperate forests of south-central Chile. Its leaves are used in ethnomedicine, the fruit is a drupe similar to that of the avocado and has not been studied. The aim of this study was to determine the cytotoxicity in leukemia cell and antibacterial activity, along with some chemical content characteristics of P. lingue fruit and leaf extracts. The antibacterial activity was determined by the inhibition of bacterial growth in liquid medium assay against Gram-positive and Gram-negative bacteria. The leukemia cell lines Kasumi-1 and Jurkat were used to evaluate the cytotoxic activity by using propidium iodide and AlamarBlue assays. Total phenolic, flavonoid, condensed tannin, alkaloid and lipid contents were evaluated in the fruit and in the leaf extracts. The antioxidant activity of both extracts were also elavaluated. Leaf extract presented the highest content of total phenols, condensed tannins and flavonoids, and also the highest antioxidant activity. While the fruit extract has a higher amount of lipids and alkaloids and the high antibacterial activity against Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, Bacillus megaterium and Micrococcus luteus. The leaf extract only showed activity against M. luteus. Concerning the cytotoxic activity, only the fruit extract showed cytotoxicity against the cell lines Jurkat and Kasumi-1. P. lingue fruit extract is a potential source of biologically active molecules for the development of new drugs to be used in some types of leukemia, as well as antibacterial agent.

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