scholarly journals Seasonal dynamics and molecular differentiation of three natural Anopheles species (Diptera: Culicidae) of the Maculatus group (Neocellia series) in malaria hotspot villages of Thailand

2020 ◽  
Vol 13 (1) ◽  
Author(s):  
Suchada Sumruayphol ◽  
Tanawat Chaiphongpachara ◽  
Yudthana Samung ◽  
Jiraporn Ruangsittichai ◽  
Liwang Cui ◽  
...  
Keyword(s):  
Seasonal Dynamics ◽  
Mosquito Control ◽  
Indian Subcontinent ◽  
Control Measures ◽  
Malaria Vectors ◽  
Its2 Region ◽  
Wet Season ◽  
Endemic Region ◽  
Clear Cut ◽  
Tak Province

Abstract Background Anopheles sawadwongporni Rattanarithikul & Green, Anopheles maculatus Theobald and Anopheles pseudowillmori (Theobald) of the Anopheles maculatus group (Diptera: Culicidae) are recognized as potential malaria vectors in many countries from the Indian subcontinent through Southeast Asia to Taiwan. A number of malaria vectors in malaria hotspot areas along the Thai-Myanmar border belong to this complex. However, the species distribution and dynamic trends remain understudied in this malaria endemic region. Methods Mosquitoes of the Maculatus group were collected using CDC light traps every other week from four villages in Tha Song Yang District, Tak Province, Thailand from January to December 2015. Adult female mosquitoes were morphologically identified on site using taxonomic keys. Molecular species identification was performed by multiplex PCR based on the internal transcribed spacer 2 (ITS2) region of ribosomal DNA (rDNA) and sequencing of the cox1 gene at a DNA barcoding region in a subset of 29 specimens. Results A total of 1328 An. maculatus (sensu lato) female mosquitoes were captured with An. maculatus, An. sawadwongporni and An. pseudowilmori accounting for 75.2, 22.1 and 2.7% respectively. The field captured mosquitoes of the Maculatus group were most abundant in the wet season and had a preferred distribution in villages at higher elevations. The phylogenetic relationships of 29 cox1 sequences showed a clear-cut separation of the three member species of the Maculatus group, with the An. pseudowillmori cluster being separated from An. sawadwongporni and An. maculatus. Conclusions This study provides updated information for the species composition, seasonal dynamics and microgeographical distribution of the Maculatus group in malaria-endemic areas of western Thailand. This information can be used to guide the planning and implementation of mosquito control measures in the pursuance of malaria transmission.

scholarly journals The regulatory genome of the malaria vector Anopheles gambiae: integrating chromatin accessibility and gene expression

2021 ◽  
Vol 3 (1) ◽  
Author(s):  
José L Ruiz ◽  
Lisa C Ranford-Cartwright ◽  
Elena Gómez-Díaz
Keyword(s):  
Gene Expression ◽  
Transcriptional Regulation ◽  
Anopheles Gambiae ◽  
Mosquito Control ◽  
Regulatory Elements ◽  
Chromatin Accessibility ◽  
Malaria Vectors ◽  
Specific Gene ◽  
Mosquito Vector ◽  
Human Malaria

Abstract Anopheles gambiae mosquitoes are primary human malaria vectors, but we know very little about their mechanisms of transcriptional regulation. We profiled chromatin accessibility by the assay for transposase-accessible chromatin by sequencing (ATAC-seq) in laboratory-reared A. gambiae mosquitoes experimentally infected with the human malaria parasite Plasmodium falciparum. By integrating ATAC-seq, RNA-seq and ChIP-seq data, we showed a positive correlation between accessibility at promoters and introns, gene expression and active histone marks. By comparing expression and chromatin structure patterns in different tissues, we were able to infer cis-regulatory elements controlling tissue-specific gene expression and to predict the in vivo binding sites of relevant transcription factors. The ATAC-seq assay also allowed the precise mapping of active regulatory regions, including novel transcription start sites and enhancers that were annotated to mosquito immune-related genes. Not only is this study important for advancing our understanding of mechanisms of transcriptional regulation in the mosquito vector of human malaria, but the information we produced also has great potential for developing new mosquito-control and anti-malaria strategies.

scholarly journals SELF-REPORTED MALARIA AND MOSQUITO AVOIDANCE IN RELATION TO HOUSEHOLD RISK FACTORS IN A KENYAN COASTAL CITY

2005 ◽  
Vol 37 (6) ◽  
pp. 761-771 ◽  
Author(s):  
JOSEPH KEATING ◽  
KATE MACINTYRE ◽  
CHARLES M. MBOGO ◽  
JOHN I. GITHURE ◽  
JOHN C. BEIER
Keyword(s):  
Rural Areas ◽  
Mosquito Control ◽  
Control Measure ◽  
Control Measures ◽  
Level Control ◽  
Cross Sectional Survey ◽  
Cross Sectional ◽  
Household Level ◽  
Household Factors ◽  
History Of

A geographically stratified cross-sectional survey was conducted in 2002 to investigate household-level factors associated with use of mosquito control measures and self-reported malaria in Malindi, Kenya. A total of 629 households were surveyed. Logistic regressions were used to analyse the data. Half of all households (51%) reported all occupants using an insecticide-treated bed net and at least one additional mosquito control measure such as insecticides or removal of standing water. Forty-nine per cent reported a history of malaria in the household. Of the thirteen household factors analysed, low (OR=0·23, CI 0·11, 0·48) and medium (OR=0·50, CI 0·29, 0·86) education, mud–wood–coral (OR=0·0·39, CI 0·24, 0·66) and mud block–plaster (OR=0·47, CI 0·25, 0·87) wall types, farming (OR=1·38, CI 1·01, 1·90) and travel to rural areas (OR=0·48, CI 0·26, 0·91) were significantly associated with the use of mosquito control, while controlling for other covariates in the model. History of reported malaria was not associated with the use of mosquito control (OR=1·22, CI 0·79, 1·88). Of the thirteen covariates analysed in the second model, only two household factors were associated with history of malaria: being located in the well-drained stratum (OR=0·49, CI 0·26, 0·96) and being bitten while in the house (OR=1·22, CI 0·19, 0·49). These results suggest that high socioeconomic status is associated with increased household-level mosquito control use, although household-level control may not be enough, as many people are exposed to biting mosquitoes while away from the house and in areas that are more likely to harbour mosquitoes.

scholarly journals Knowledge, Attitudes, Practices and its associated risk factors related to Cutaneous Leishmaniasis in Ilam province of Iran

2022 ◽  
Vol 3 (1) ◽  
pp. 01-14
Author(s):  
Fatima Ibrahim ABDULSALAM ◽  
◽  
Tabarak Malik ◽  
Keyword(s):  
Cutaneous Leishmaniasis ◽  
Intervention Program ◽  
Control Measures ◽  
Endemic Region ◽  
Attitudes And Practices ◽  
Disease Occurrence ◽  
Kap Survey ◽  
Border Town ◽  
Associated Risk Factors ◽  
And Control

Knowledge, Attitudes and Practices (KAP) surveys precede an awareness or intervention program, it addresses a felt need in a population in which that need exists. In an endemic region of cutaneous leishmaniasis disease occurrence, public enlightenment on its preventive and control measures is highly important. Ilam province of Iran is a provincial border town transited annually by pilgrims was reported to have the most cases of cutaneous leishmaniasis ranking highest since 2010 yet no report on KAP survey has been previously conducted.

scholarly journals Emergence of West Nile virus infections in humans in Turkey, 2010 to 2011

2012 ◽  
Vol 17 (21) ◽  
Author(s):  
H Kalaycioglu ◽  
G Korukluoglu ◽  
A Ozkul ◽  
O Oncul ◽  
S Tosun ◽  
...  
Keyword(s):  
West Nile Virus ◽  
Mosquito Control ◽  
Epidemiological Studies ◽  
Control Measures ◽  
Lower Activity ◽  
Virus Infections ◽  
West Nile ◽  
Notifiable Diseases ◽  
Routine Surveillance

In 2010, 47 human cases of West Nile virus (WNV) infection, including 12 laboratory-confirmed and 35 probable cases, were identified in Turkey. These were the first cases detected during routine surveillance. The patients were from 15 provinces, mainly located in the western part of the country. Incidence was 0.19/100,000 with a maximum of 1.39 in Sakarya province. Forty of the total 47 cases showed neuroinvasive manifestation. Median age was 58 years with a range of four to 86. Ten of the patients died. Enhanced surveillance in humans and animals and mosquito control measures were implemented. The WNV infections were included in the national notifiable diseases list as of April 2011. In 2011, three probable and two confirmed cases of WNV infection were diagnosed in provinces where infections had been detected in the previous year, supporting a lower activity than 2010. However, detection of WNV infections in humans in 2010 and 2011 consecutively, may indicate that WNV has become endemic in the western part of Turkey. Field epidemiological studies were undertaken to understand more about the nature of infection in Turkey.

scholarly journals Co-circulation of Chikungunya and Dengue viruses in Dengue endemic region of New Delhi, India during 2016

2018 ◽  
Vol 146 (13) ◽  
pp. 1642-1653 ◽  
Author(s):  
M. Hisamuddin ◽  
A. Tazeen ◽  
M. Abdullah ◽  
M. Islamuddin ◽  
N. Parveen ◽  
...  
Keyword(s):  
Viral Infections ◽  
Purifying Selection ◽  
Control Measures ◽  
Effective Control ◽  
New Delhi ◽  
Emerging Pathogens ◽  
Endemic Region ◽  
High Entropy ◽  
Genotype Iii ◽  
Two Samples

AbstractCo-circulation of Chikungunya and Dengue viral infections (CHIKV and DENV) have been reported mainly due to transmission by commonAedesvector. The purpose of the study was to identify and characterise the circulating strains of CHIKV and DENV in DENV endemic region of New Delhi during 2016. CHIKV and DENV were identified in the blood samples (n= 130) collected from suspected patients by RT-PCR. CHIKV was identified in 26 of 65 samples (40%). Similarly, DENV was detected in 48 of 120 samples (40%). Co-infection with both the viruses was identified in five (9%) of the samples. Interestingly, concurrent infection with DENV, CHIKV andPlasmodium vivaxwas detected in two samples. CHIKV strains (n= 11) belonged to the ECSA genotype whereas DENV-3 sequences (n= eight) clustered in Genotype III by phylogenetic analysis. Selection pressure of E1 protein of CHIKV and CprM protein of DENV-3 revealed purifying selection with four and two positive sites, respectively. Four amino acids of the CHIKV were positively selected and had high entropy suggesting probable variations. Co-circulation of both viruses in DENV endemic regions warrants effective monitoring of these emerging pathogens via comprehensive surveillance for implementation of effective control measures.

scholarly journals A Comprehensive Analysis on Abundance, Distribution, and Bionomics of Potential Malaria Vectors in Mannar District of Sri Lanka

2019 ◽  
Vol 2019 ◽  
pp. 1-13
Author(s):  
Nayana Gunathilaka ◽  
Menaka Hapugoda ◽  
Rajitha Wickremasinghe ◽  
Wimaladharma Abeyewickreme
Keyword(s):  
Waste Water ◽  
Sri Lanka ◽  
Current Knowledge ◽  
Breeding Habitat ◽  
Control Measures ◽  
Malaria Vectors ◽  
Detailed Knowledge ◽  
Lagoon Water ◽  
Breeding Habitats ◽  
The Mean

Background. A detailed knowledge of the distribution of the malaria vectors in Mannar district of Sri Lanka has not been studied after 1927. Past records indicated the presence of only seven species of anophelines, namely, An. culicifacies, An. subpictus, An. barbirostris, An. peditaeniatus, An. nigerrimus, An. Jamesii, and An. maculatus. There have been many changes in terms of distribution of Anopheles in the district over time. Methods. Entomological surveillance was conducted on a monthly basis, comprising indoor hand collection, window trap collection, cattle-baited net collection, cattle-baited hut collection, and larval survey from June 2010 to June 2012 in 12 study areas under three entomological sentinel sites. The relationship between seven abiotic variables of the breeding habitats was measured. Pearson’s correlation coefficients were used to determine the associations between climatic variables and anopheline densities. Results. A total of 74,181 mosquitoes belonging to 14 Anopheles species were recorded. An. subpictus was the most predominant species from all techniques representing 92% (n=68,268) of the total anopheline collection. However, Anopheles culicifacies was not recorded from any site during the study period. Larval surveys identified 12 breeding habitat categories including waste water collections, lagoon water collections, and drains which were not recorded as breeding habitats by previous studies. The mean dissolved oxygen level of waste water collections was 3.45±0.15 mg/l. The mean salinity and conductivity of lagoon water collections were 21105±1344 mg/l and 34734±1974 μs/cm, respectively. Conclusion. The present study provides the updated knowledge on anopheline distribution and vector bionomics. Therefore, documentation of the current knowledge would be useful for learners and health authorities to design appropriate vector control measures in the prevention of reintroduction of malaria.

scholarly journals Resting behaviour of malaria vectors in highland and lowland sites of western Kenya: Implication on malaria vector control measures

PLoS ONE ◽  
2020 ◽  
Vol 15 (2) ◽  
pp. e0224718 ◽  
Author(s):  
Maxwell G. Machani ◽  
Eric Ochomo ◽  
Fred Amimo ◽  
Jackline Kosgei ◽  
Stephen Munga ◽  
...  
Keyword(s):  
Vector Control ◽  
Malaria Vector ◽  
Control Measures ◽  
Malaria Vectors ◽  
Malaria Vector Control ◽  
Western Kenya ◽  
Resting Behaviour

Indian Missions in Russia in the Late Nineteenth Century and British Historiography of International Relations in Asia

1987 ◽  
Vol 21 (4) ◽  
pp. 639-646 ◽  
Author(s):  
N. A. Khalfin
Keyword(s):  
Nineteenth Century ◽  
Twentieth Century ◽  
International Relations ◽  
Indian Subcontinent ◽  
Late Nineteenth Century ◽  
Clear Cut ◽  
Late Nineteenth

The British Empire's colonial possession of India for many decades of the nineteenth century and in the first half of the twentieth century largely determined the Empire's economic and political pattern. Numerous books and articles on this subject have been written and still more speeches have been delivered, but the most clear-cut and all-round assessment of the significance for Britain of all-out exploitation of the Indian subcontinent and its population was given by Lord Curzon.

Current status of Plasmodium knowlesi vectors: a public health concern?

Parasitology ◽  
2016 ◽  
Vol 145 (1) ◽  
pp. 32-40 ◽  
Author(s):  
I. VYTHILINGAM ◽  
M. L. WONG ◽  
W. S. WAN-YUSSOF
Keyword(s):  
Public Health ◽  
Plasmodium Knowlesi ◽  
Point Of View ◽  
Control Measures ◽  
Health Concern ◽  
Current Status ◽  
Malaria Vectors ◽  
Future Studies ◽  
Simian Malaria ◽  
And Control

SUMMARYPlasmodium knowlesi a simian malaria parasite is currently affecting humans in Southeast Asia. Malaysia has reported the most number of cases and P. knowlesi is the predominant species occurring in humans. The vectors of P. knowlesi belong to the Leucosphyrus group of Anopheles mosquitoes. These are generally described as forest-dwelling mosquitoes. With deforestation and changes in land-use, some species have become predominant in farms and villages. However, knowledge on the distribution of these vectors in the country is sparse. From a public health point of view it is important to know the vectors, so that risk factors towards knowlesi malaria can be identified and control measures instituted where possible. Here, we review what is known about the knowlesi malaria vectors and ascertain the gaps in knowledge, so that future studies could concentrate on this paucity of data in-order to address this zoonotic problem.

scholarly journals Genetic Control of Mosquitoes: population suppression strategies

2012 ◽  
Vol 54 (5) ◽  
pp. 287-292 ◽  
Author(s):  
André Barretto Bruno Wilke ◽  
Mauro Toledo Marrelli
Keyword(s):  
Genetic Control ◽  
Mosquito Control ◽  
Public Health Problem ◽  
Mass Rearing ◽  
Control Measures ◽  
Reproductive Capacity ◽  
Lethal Gene ◽  
Control Methods ◽  
Species Specific ◽  
Population Suppression

Over the last two decades, morbidity and mortality from malaria and dengue fever among other pathogens are an increasing Public Health problem. The increase in the geographic distribution of vectors is accompanied by the emergence of viruses and diseases in new areas. There are insufficient specific therapeutic drugs available and there are no reliable vaccines for malaria or dengue, although some progress has been achieved, there is still a long way between its development and actual field use. Most mosquito control measures have failed to achieve their goals, mostly because of the mosquito's great reproductive capacity and genomic flexibility. Chemical control is increasingly restricted due to potential human toxicity, mortality in no target organisms, insecticide resistance, and other environmental impacts. Other strategies for mosquito control are desperately needed. The Sterile Insect Technique (SIT) is a species-specific and environmentally benign method for insect population suppression, it is based on mass rearing, radiation mediated sterilization, and release of a large number of male insects. Releasing of Insects carrying a dominant lethal gene (RIDL) offers a solution to many of the drawbacks of traditional SIT that have limited its application in mosquitoes while maintaining its environmentally friendly and species-specific utility. The self-limiting nature of sterile mosquitoes tends to make the issues related to field use of these somewhat less challenging than for self-spreading systems characteristic of population replacement strategies. They also are closer to field use, so might be appropriate to consider first. The prospect of genetic control methods against mosquito vectored human diseases is rapidly becoming a reality, many decisions will need to be made on a national, regional and international level regarding the biosafety, social, cultural and ethical aspects of the use and deployment of these vector control methods.

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