scholarly journals Fermented rye with Agaricus subrufescens and mannan-rich hydrolysate based feed additive to modulate post-weaning piglet immune response

2021 ◽  
Vol 7 (1) ◽  
Author(s):  
Nienke de Groot ◽  
Fernando Fariñas ◽  
Lluís Fabà ◽  
Ellen Hambrecht ◽  
Carolina G. Cabrera-Gómez ◽  
...  
Keyword(s):  
Immune Modulation ◽  
Pathogenic Bacteria ◽  
Transforming Growth Factor ◽  
Cytokine Gene Expression ◽  
Feed Additive ◽  
Copra Meal ◽  
Gastrointestinal Health ◽  
Agaricus Subrufescens ◽  
Weaning Piglets ◽  
Nutritional Strategies

Abstract Background The process of weaning in piglets is often associated with an increased inflammation response in the intestine and compromised intestinal integrity and morphology, favoring a delay in intestinal maturation and a predisposal to diseases. Research has shown the potential of different nutritional strategies to reduce the production of pro-inflammatory cytokines, with the main goal to manipulate health and performance of pigs. Promising examples of nutritional strategies are fungal fermented products and their derivatives which are described to contain several compounds that may play a role in gastrointestinal health and pathogenic bacteria control. Products from Agaricus subrufescens mushroom are reported to contain prophylactic and therapeutic properties including antimicrobial and immunomodulatory properties. Results This study analysed the post-weaning immune status in intestinal tissue and blood of piglets, with the objective to evaluate the gastrointestinal health and immune modulation response induced by a blend of mannan-rich hydrolyzed copra meal and fermented rye with A. subrufescens. Intestinal histomorphology demonstrated a villus height reduction in jejunum and increase in ileum on day 15, while increased villous height in jejunum and ileum on day 30. The results showed that in post-weaning piglets, the feed additive stimulates an immunomodulation effect most evident at 15 days post-weaning, with significant lower expression of cytokines Interferon (IFN) γ, Interleukin (IL) 1α, IL-1β, IL-6, IL-8, IL-10 and Transforming Growth Factor (TGF) β in jejunum, accompanied with an increase in peripheral blood mononuclear cells (PBMC) cytokine gene expression of IL-1β, IL-6, IL-8, IL-10, IL-12p35 (IL-12α), IL-12p40 (IL-12β), Tumor Necrosis Factor (TNF) α, IFN-α, and TGF-β. In piglets fed the feed additive, the quantity of Immunoglobulin (Ig) A producing cells in jejunum, ileum was reduced on day 15 and 30 post-weaning, and on day 30 and 45 post-weaning in colon tissue. Natural Killer (NK) cells count in blood were increased on day 15 post-weaning in the piglets fed the feed additive. Conclusion This study implies the potential of the blend including mannan-rich hydrolyzed copra meal and fermented rye with A. subrufescens on immune modulation in the intestine of post-weaning piglets.

Piglets' gut microbiota dynamics.

2021 ◽  
Vol 16 (048) ◽  
Author(s):  
Daniela R. Klein
Keyword(s):  
Gut Microbiota ◽  
Pathogenic Bacteria ◽  
Cell Structure ◽  
Animal Health ◽  
Swine Production ◽  
Growth Promoters ◽  
Health Maintenance ◽  
Mortality And Morbidity ◽  
Weaning Piglets ◽  
The Impact

Abstract The gut microbiota has been a subject of great interest in recent years because the composition and diversity are associated with the maintenance of piglets' health and welfare. This review aims to summarise the composition and diversity of piglet microbiome, the impact on health maintenance, influence of feed and nutrients, impact of stress situations, and the effect of growth promoters and antimicrobials on gut microbiota. The composition and diversity of microbiota are influenced by animal early experiences, the appropriate development of microbiota is essential for intestinal function, and influence animal health, growth and productivity. Interactions between the gut microbiota and the immune system help maintain epithelial barrier, and protect from post-weaning diarrhoea pathogenies. After weaning, the piglets' diet changes abruptly, affecting the microbiota and the physiology, but this can be modulated through nutrients such as fibre, protein and minerals. Stress situations contribute to the appearance of intestinal disorders, possibly changing the microbiota and epithelial cell structure, facilitating colonisation of pathogenic bacteria, decreased performance and increase the use of antimicrobials. In swine production, growth promoters and antibiotics are used to reduce mortality and morbidity, especially in weaning piglets, reducing and controlling potential pathogenic bacteria, resulting in more feed intake and body weight. Antimicrobial use reduces the entire gut microbial population; the replacers are probiotics, prebiotics and organic acids, which helps maintain intestinal microbial populations, and inhibits pathogenic bacteria development. Knowing the animal microbiome dynamics helps improve immunity, productive performance and welfare, and also reduce the use of antimicrobials in animal production.

Potential use of beta-mannan from copra meal as a feed additive for broilers

2012 ◽  
Vol 68 (4) ◽  
pp. 707-716 ◽  
Author(s):  
B. Sundu ◽  
U. Hatta ◽  
A.S. Chaudhry
Keyword(s):  
Feed Additive ◽  
Copra Meal ◽  
Potential Use

Cytokine Gene Expression in Nasal Polyps

1998 ◽  
Vol 107 (8) ◽  
pp. 665-670 ◽  
Author(s):  
Chul Hee Lee ◽  
Chae-Seo Rhee ◽  
Yang-Gi Min
Keyword(s):  
Gene Expression ◽  
Southern Blot ◽  
Nasal Polyp ◽  
Nasal Polyps ◽  
Transforming Growth Factor ◽  
Cytokine Gene Expression ◽  
Messenger Rnas ◽  
Gene Expressions ◽  
Ifn Γ ◽  
Density Ratios

Nasal polyps are the most common mass lesion of the nasal cavity. Various pathogenetic mechanisms have been proposed. However, the cause is still largely unknown, and treatment methods have not been changed for several hundred years. In order to investigate the role of cytokines in the pathogenesis of nasal polyps, expression of cytokine messenger RNAs (mRNAs) in nasal polyps was investigated. We performed reverse transcriptase-polymerase chain reaction and Southern blot to examine gene expression of the cytokines interleukin (IL)-1ß, IL-6, IL-8, transforming growth factor (TGF)-ß, IL-4, IL-5, and interferon (IFN)-Γ and compared the results with the gene expressions of these cytokines in normal nasal mucosa. Nasal polyp tissues were obtained from 14 patients undergoing polypectomy for nasal obstruction. Among them, 4 patients suffered from associated perennial allergic rhinitis. The mRNAs of IL-4 and IL-5 (Th2 cytokines) as well as IFN-γ (Thl cytokine) were expressed in all of the nasal polyps obtained from the 14 patients, irrespective of the presence or absence of allergy, while 2, 0, and 4 of 6 normal turbinate mucosae expressed IL-4, IL-5, and IFN-γ mRNAs, respectively. The mRNAs of IL-1ß, IL-6, IL-8, and TGF-ß were expressed in 6, 1, 2, and 3 of 6 normal turbinate mucosae, respectively, while the mRNAs of these cytokines were expressed in all of the 14 polyp tissues except IL-6 mRNA, which was expressed in 13 nasal polyp tissues. There were no differences in the mean density ratios of each cytokine band on Southern blot between polyp tissues with allergy and those without allergy. These results suggest that many cytokines are produced in nasal polyps, that they may play important roles in the pathogenesis of nasal polyps, and that allergy per se may not play a fundamental role in the pathogenesis of nasal polyps.

scholarly journals Effects of Lactobacilli on Cytokine Expression by Chicken Spleen and Cecal Tonsil Cells

2010 ◽  
Vol 17 (9) ◽  
pp. 1337-1343 ◽  
Author(s):  
Jennifer T. Brisbin ◽  
Joshua Gong ◽  
Payvand Parvizi ◽  
Shayan Sharif
Keyword(s):  
Gene Expression ◽  
Inflammatory Response ◽  
Mononuclear Cells ◽  
Transforming Growth Factor ◽  
Lactobacillus Reuteri ◽  
Expression Profiles ◽  
Cytokine Gene Expression ◽  
Host Immune System ◽  
T Helper 1 ◽  
Cecal Tonsil

ABSTRACT Lactobacillus acidophilus, Lactobacillus reuteri, and Lactobacillus salivarius are all normal residents of the chicken gastrointestinal tract. Given the interest in using probiotic bacteria in chicken production and the important role of the microbiota in the development and regulation of the host immune system, the objective of the current study was to examine the differential effects of these bacteria on cytokine gene expression profiles of lymphoid tissue cells. Mononuclear cells isolated from cecal tonsils and spleens of chickens were cocultured with one of the three live bacteria, and gene expression was analyzed via real-time quantitative PCR. All three lactobacilli induced significantly more interleukin 1β (IL-1β) expression in spleen cells than in cecal tonsil cells, indicating a more inflammatory response in the spleen than in cecal tonsils. In cecal tonsil cells, substantial differences were found among strains in the capacity to induce IL-12p40, IL-10, IL-18, transforming growth factor β4 (TGF-β4), and gamma interferon (IFN-γ). In conclusion, we demonstrated that L. acidophilus is more effective at inducing T-helper-1 cytokines while L. salivarius induces a more anti-inflammatory response.

scholarly journals Maternal supplementation of seaweed-derived polysaccharides improves intestinal health and immune status of suckling piglets

2015 ◽  
Vol 4 ◽  
Author(s):  
G. Heim ◽  
J. V. O'Doherty ◽  
C. J. O'Shea ◽  
D. N. Doyle ◽  
A. M. Egan ◽  
...  
Keyword(s):  
Gene Expression ◽  
Dietary Treatment ◽  
Intestinal Morphology ◽  
Cytokine Gene Expression ◽  
Cytokine Gene ◽  
Lps Challenge ◽  
Nutrient Transporter ◽  
Suckling Piglets ◽  
Small Intestinal ◽  
Weaning Piglets

AbstractThe experiment investigated the effect of maternal dietary supplementation of seaweed-derived polysaccharides (SDP) (–SDPv.+SDP,n   20) from day 83 of gestation until weaning (day 28) on selected sow faeces and piglet digesta microbiota populations, piglet small-intestinal morphology, and intestinal nutrient transporter and inflammatory cytokine gene expression at birth, 48 h after birth and weaning. The effect of maternal dietary treatment on the piglet gene expression profile of inflammatory cytokines in the colon following a lipopolysaccharide (LPS) challenge was also investigated. Dietary SDP reduced sow faecal Enterobacteriaceae gene numbers at parturition. Small-intestinal morphology, nutrient transporter and cytokine gene expression in newborn piglets did not differ between maternal dietary treatments (P > 0·10). At 48 h after birth, sodium–glucose-linked transporter 1 gene expression was down-regulated in the ileum of piglets suckling the SDP-supplemented sows compared with those suckling the basal sows (P = 0·050). There was a SDP × LPS challenge interaction onIL-1andIL-6gene expression in the colon of piglets (P < 0·05). The gene expression ofIL-1andIL-6was down-regulated in the LPS-challenged colon of piglets suckling the SDP sows compared with those suckling the basal sows (P < 0·05). However, there was no difference inIL-1andIL-6gene expression in the unchallenged colon between treatment groups. At weaning, piglets suckling the SDP-supplemented sows had increased villus height in the jejunum and ileum compared with those suckling the basal-fed sows (P < 0·05). In conclusion, maternal dietary SDP supplementation enhanced the immune response of suckling piglets and improved gut morphology, making them more immune competent to deal with post-weaning adversities.

Differential cytokine response from dendritic cells to commensal and pathogenic bacteria in different lymphoid compartments in humans

2006 ◽  
Vol 290 (4) ◽  
pp. G839-G845 ◽  
Author(s):  
Liam O’Mahony ◽  
Louise O’Callaghan ◽  
Jane McCarthy ◽  
David Shilling ◽  
Paul Scully ◽  
...  
Keyword(s):  
Dendritic Cells ◽  
Immune System ◽  
Peripheral Blood ◽  
Pathogenic Bacteria ◽  
Mononuclear Cells ◽  
Cytokine Production ◽  
Transforming Growth Factor ◽  
Mesenteric Lymph Nodes ◽  
Tnf Α

Resident host microflora condition and prime the immune system. However, systemic and mucosal immune responses to bacteria may be divergent. Our aim was to compare, in vitro, cytokine production by human mononuclear and dendritic cells (DCs) from mesenteric lymph nodes (MLNs) and peripheral blood mononuclear cells (PBMCs) to defined microbial stimuli. Mononuclear cells and DCs isolated from the MLN ( n = 10) and peripheral blood ( n = 12) of patients with active colitis were incubated in vitro with the probiotic bacteria Lactobacillus salivarius UCC118 or Bifidobacterium infantis 35624 or the pathogenic organism Salmonella typhimurium UK1. Interleukin (IL)-12, tumor necrosis factor (TNF)-α, transforming growth factor (TGF)-β, and IL-10 cytokine levels were quantified by ELISA. PBMCs and PBMC-derived DCs secreted TNF-α in response to the Lactobacillus, Bifidobacteria, and Salmonella strains, whereas MLN cells and MLN-derived DCs secreted TNF-α only in response to Salmonella challenge. Cells from the systemic compartment secreted IL-12 after coincubation with Salmonella or Lactobacilli, whereas MLN-derived cells produced IL-12 only in response to Salmonella. PBMCs secreted IL-10 in response to the Bifidobacterium strain but not in response to the Lactobacillus or Salmonella strain. However, MLN cells secreted IL-10 in response to Bifidobacteria and Lactobacilli but not in response to Salmonella. In conclusion, commensal bacteria induced regulatory cytokine production by MLN cells, whereas pathogenic bacteria induce T cell helper 1-polarizing cytokines. Commensal-pathogen divergence in cytokine responses is more marked in cells isolated from the mucosal immune system compared with PBMCs.

scholarly journals Growth differentiation factor-15 is associated with age-related monocyte immunosenescence

2020 ◽  
Author(s):  
Brandt D. Pence ◽  
Johnathan R. Yarbro ◽  
Russell S. Emmons
Keyword(s):  
Mitochondrial Function ◽  
Transforming Growth Factor Beta ◽  
Transforming Growth Factor ◽  
Immune Cell ◽  
Inflammatory Responses ◽  
Cell Subset ◽  
Cytokine Gene Expression ◽  
Growth Differentiation Factor 15 ◽  
Differentiation Factor ◽  
Age Related

ABSTRACTBackgroundImmunosenescence is an age-associated decrease in function of immune cells precipitated by a variety of mechanisms and affecting nearly every immune cell subset. In myeloid cell subsets, aging reduces numbers of phagocytes and impairs their functional abilities, including antigen presentation, phagocytosis, and bacterial clearance. Recently, we have described an aging effect on several functions indicating immunosenescence in monocytes, including impaired mitochondrial function and reduced inflammatory cytokine gene expression during stimulation with lipopolysaccharide (LPS). We hypothesized that circulating factors altered by the aging process underly these changes. Growth/differentiation factor-15 (GDF-15) is a distant member of the transforming growth factor-beta superfamily that has known anti-inflammatory effects in macrophages and has recently been shown to be highly differentially expressed during aging. We used biobanked serum and plasma samples to assay circulating GDF-15 levels in subjects from our previous studies and examined correlations between GDF-15 levels and monocyte mitochondrial function and inflammatory responses.ResultsMonocyte interleukin-6 production due to lipopolysaccharide stimulation was negatively correlated to plasma GDF-15 levels. Additionally, serum GDF-15 was positively correlated to circulating CD16+ monocyte proportions and negatively correlated to monocyte mitochondrial respiratory capacity.ConclusionsThe results of these analyses suggest that GDF-15 is a potential circulating factor affecting a variety of monocyte functions and promoting monocyte immunosenescence, and thus may be an attractive candidate for therapeutic intervention to ameliorate this.

scholarly journals Effects of hydrolyzed yeast supplementation on growth performance, immunity, antioxidant capacity, and microbial shedding in weaning pigs

2020 ◽  
Vol 13 (9) ◽  
pp. 1902-1909
Author(s):  
Waewaree Boontiam ◽  
Chalong Wachirapakorn ◽  
Phreerapong Phaengphairee
Keyword(s):  
Antioxidant Capacity ◽  
Growth Performance ◽  
Pathogenic Bacteria ◽  
Average Daily Gain ◽  
Basal Diet ◽  
Feed Additive ◽  
Feed Ratio ◽  
Salmonella Spp ◽  
Large White ◽  
Weaning Pigs

Background and Aim: Weaning pigs normally suffer from many stressors which have impaired growth performance and immunity. Hydrolyzed yeast has been proposed as an alternative feed additive. The aim of this study was to investigate the effects of various levels of hydrolyzed yeast (HY) supplementation in the feed of weaning pigs on growth performance, diarrhea incidence, immunity, antioxidant capacity, and microbial populations. Materials and Methods: A total of 144 crossbred weaning pigs (Duroc × Landrace × Large White) with a mean body weight (BW) of 7.46 kg were randomly assigned to one of four treatments during a 5-week feeding trial. Treatments consisted of a basal diet without HY inclusion (control), or the basal diet supplemented with HY at 0.5, 1.0, and 1.5 g/kg of diet, respectively. Results: Piglets fed with 1.0 or 1.5 g/kg HY presented significantly increased BW (p=0.009) and decreased incidence of diarrhea (p=0.001). The final BW (p=0.012), average daily gain (p=0.094), and average daily feed intake (p=0.091) showed a linear improvement with the level of HY inclusion. However, the gain-to-feed ratio was unaffected by dietary treatments. Linear responses to the HY supplementation levels were also observed for blood urea nitrogen (p=0.030), total protein (p=0.017), lymphocyte percentage (p=0.064), catalase activity (p=0.089), malondialdehyde (MDA) level (p=0.001), Salmonella spp. (p=0.024), Escherichia coli (p=0.021), and Lactobacillus spp. (p=0.048). Dietary inclusion of HY at 1.0 and 1.5 g/kg resulted in increased immunoglobulin A and G secretions (p=0.042 and p=0.022, respectively) and decreased MDA concentration (p<0.01) and Salmonella spp. (p=0.026) and E. coli (p=0.050). Conclusion: It was concluded that HY inclusion at 1.0 and 1.5 g/kg in the diet of weaning pigs improve BW, immunoglobulin secretion, and antioxidant enzyme activity, whereas it lowers diarrhea occurrence, lipid peroxidation, and pathogenic bacteria in weaning pigs.

scholarly journals Identification and quantification of tylosin in animal feed by liquid chromatography combined with electrospray ionisation mass spectrometry

2020 ◽  
Vol 64 (2) ◽  
pp. 299-304
Author(s):  
Monika Przeniosło-Siwczyńska ◽  
Aleksandra Grelik ◽  
Krzysztof Kwiatek
Keyword(s):  
Liquid Chromatography ◽  
Pathogenic Bacteria ◽  
Animal Feed ◽  
Solid Phase ◽  
Feed Additive ◽  
Electrospray Ionisation ◽  
Specificity And Sensitivity ◽  
Feed Samples ◽  
Ionisation Mass ◽  
Identification And Quantification

AbstractIntroductionThe authorisation of tylosin as feed additive was withdrawn for reasons of human health concerning resistance of pathogenic bacteria. An analytical method for the identification and quantification of tylosin in animal feed was developed and validated.Material and MethodsThe samples were extracted using an acidified methanol:water mixture and solid-phase extraction was employed for the isolation of the antibiotic from diluted feed samples. Tylosin was analysed by liquid chromatography with electrospray ionisation mass spectrometric detection. The method’s performance was evaluated in adherence to the Commission Decision 2002/657/EC.ResultsThe recovery of the analyte from spiked samples was determined to be in the range from 78.9% to 108.3% depending on tylosin concentrations. The CCα and CCβ values for tylosin in feeds were determined at 0.085 mg kg-1 and 0.091 mg kg-1, respectively. The method detection limit was found to be 0.035 mg kg-1 and the quantification limit 0.05 mg kg-1. The applicability of the developed method was tested by analysing real feed samples.ConclusionA reliable LC-MS method was developed to identify and quantify tylosin in animal feed with a good repeatability and a high specificity and sensitivity. Because of these characteristics, the proposed method is applicable and could be deemed necessary within the field of feed control and safety.

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