scholarly journals Quercetin Modulates Behavioural and Biochemical Alterations in Stressed Mice

2021 ◽  
Vol 18 (4) ◽  
pp. 681-689
Author(s):  
Anthony Taghogho Eduviere ◽  
Emuesiri Goodies Moke ◽  
Adrian Itivere Omogbiya ◽  
Lily Oghenevovwero Otomewo ◽  
Juliet Nnenda Olayinka ◽  
...  
Keyword(s):  
Prefrontal Cortex ◽  
Sleep Deprivation ◽  
Neuroprotective Effect ◽  
The Body ◽  
Sleep Disruption ◽  
Control Group ◽  
Protective Effects ◽  
Active Sleep ◽  
Metabolic System ◽  
Biochemical Alterations

Disruption of the active phase of sleep alters the physiological homeostasis of the body and results in oxidative breakdown which may trigger a wide array of defects. The central nervous system and the metabolic system are some of the most affected systems as described in several literatures. Some plant based compounds with antioxidant property have been previously described in the abrogation of the deleterious effects of active sleep disruption. One of such compounds is quercetin. This study was premeditated to expatiate on the probable neuroprotective effect of quercetin on mice exposed to 72hr active sleep disruption. Mice were allotted into five treatment groups (n = 6): group 1 served as control, group 2 received 10 mL/kg vehicle, groups 3 and 4 received 25 and 50 mg/kg quercetin respectively, and group 5 received 50 mg/kg astaxanthin. Treatment lasted for 7 days while groups 2-5 were exposed to the sleep deprivation protocol starting from day 4. Behavioural tests followed by biochemical assays and histopathological changes in the prefrontal cortex were evaluated. Data were analysed by ANOVA set at p<0.05 significance. The results revealed that quercetin, in both doses, significantly amplified memory performance, attenuated depression-like behaviour, replenished catalase and superoxide dismutase, attenuated nitric oxide levels in brain and liver of mice when compared to control group and protected against loss of prefrontal cortex neurons. In conclusion, quercetin possesses protective effects against sleep deprivation-induced brain damage.

scholarly journals Neurological Alterations and Testicular Damages in Aging Induced by D-Galactose and Neuro and Testicular Protective Effects of Combinations of Chitosan Nanoparticles, Resveratrol and Quercetin in Male Mice

Coatings ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 435
Author(s):  
Reham Z. Hamza ◽  
Mohammad S. Al-Harbi ◽  
Munirah A. Al-Hazaa
Keyword(s):  
Oxidative Stress ◽  
Chitosan Nanoparticles ◽  
Oxidative Stress Marker ◽  
Control Group ◽  
Antioxidant Enzyme Activities ◽  
Enzymatic Antioxidants ◽  
Protective Effects ◽  
Biochemical Alterations ◽  
Wide Range ◽  
Neurological Alterations

Aging is a neurological disease that is afforded by incidence of oxidative stress. Chitosan has received global interests due to its wide medical uses. Quercetin (Q) is a bioflavonoid and widely distributed in vegetables and fruits. Resveratrol is considered as a potent antioxidant and is a component of a wide range of foods. The using of either chitosan nanopartciles (CH-NPs), querectin (Q), and resveratrol (RV) to reduce the oxidative stress and biochemical alterations on brain and testicular tissues induced by D-galactose (DG) (100 mg/Kg) were the aim of the present study. This study investigated the probable protective effects of CH-NPs in two doses (140,280 mg/Kg), Q (20 mg/Kg) and RV (20 mg/Kg), against DG induced aging and neurological alterations. Brain antioxidant capacity as malonaldehyde (MDA), catalase (CAT), and glutathione reductase (GRx), as well as histopathological damages of the brain and testicular tissues were measured. The DG treated group had significantly elevated the oxidative stress markers by 96% and 91.4% in brain and testicular tissues respectively and lower significantly the antioxidant enzyme activities of both brain and testicular tissues than those of the control group by 86.95%, 69.27%, 83.07%, and 69.43%. Groups of DG that treated with a combination of CH-NPs in two doses, Q and RV, the levels of oxidative stress marker declined significantly by 68.70%, 76.64% in brain tissues and by 74.07% and 76.61% in testicular tissues, and the enzymatic antioxidants increased significantly by 75.55%, 79.24%, 62.32%, and 61.97% as compared to the DG group. The present results indicate that CH-NPs, Q, and RV have protective effects against DG-induced brain and testis tissue damage at the biochemical and histopathological levels. Mechanisms of this protective effect of used compounds against neurological and testicular toxicity may be due to the enhanced brain and testis antioxidant capacities.

scholarly journals Ameliorating Effect of L-arginine and Insulin on Streptozotocin-induced Adrenal Gland Injury in Albino Rats

1969 ◽  
Vol 11 (3) ◽  
pp. 162-168
Author(s):  
Yasmeen Mahar ◽  
Alisha Qamar ◽  
lnayatullah ◽  
Sarwath Fatimee ◽  
Mohammad Fawad Saeeduddin ◽  
...  
Keyword(s):  
Adrenal Gland ◽  
Adrenal Cortex ◽  
Treated Group ◽  
The Body ◽  
Control Group ◽  
Albino Rats ◽  
Endocrine Gland ◽  
Protective Effects ◽  
Frozen Sections ◽  
Group B

Background:Use of dietary supplements to treat illnesses has increasedtremendously in recentyears.Adrenal gland is one ofthemost commonly damaged endocrine gland in the body, not only by chemical or radiation injuries, but also as a result of differenttypes of stress.Search is underway for use ofnatural foods for protection of adrenal gland from different types ofinsults.Objective: To determine the protective effects of L-arginine on streptozotocin (STZ)-induced adrenal gland injury in albino rats,andto compare its efficacy to insulin.Material and Methods: This prospective experimental study was done at BMSI, JPMC, Karachi. Forty male, healthy albino rats,90-120 days old were segregated into 4 groups. Group A was marked as control, group B was administered STZ, group C and Dwere treated with STZ along with insulin and L-arginine respectively. At the end of study period, i.e., 6 weeks, animals weresacrificed under ether anaesthesia. Tissue from the left adrenal gland was processed for frozen sectioning to observe fat content ofthe adrenal cortex by applying OilRed O stain.Results: Oil Red-0 stained frozen sections revealed closely aggregated fat globules in adrenal cortex of STZ treated group B ascompared to control. Moderate betterment was seen in group C and in group D Oil Red O stained frozen sections as compared toSTZ treated group B.Conclusion: The results ofthe study demonstrated adrenal cortex injury by STZ which ameliorated with concomitant use of insulinandL-arginine. The protection was more pronounced with L-arginine as comparedto insulin.Keywords:STZ, adrenal gland,insulin,L-arginine

Protective effects of dietary omega-3 fatty acid supplementation on organophosphate poisoning

2017 ◽  
Vol 34 (2) ◽  
pp. 69-82 ◽  
Author(s):  
Bahattin Avci ◽  
S. Sirri Bilge ◽  
Gokhan Arslan ◽  
Omer Alici ◽  
Ozge Darakci ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Olive Oil ◽  
The Body ◽  
Control Group ◽  
Organophosphate Poisoning ◽  
Protective Effects ◽  
Omega 3 ◽  
Sprague Dawley ◽  
Omega 3 Fatty Acid ◽  
Locomotor Activities

In this study, we aimed to study the possible preventive effect of docosahexaenoic acid (DHA), a dietary omega-3 fatty acid, on toxicity caused by chlorpyrifos (CPF). Six groups of Sprague Dawley rats (200–250 g) consisting of equal numbers of males and females (n = 8) were assigned to study. The rats were orally given for 5 days. The control group was administered pure olive oil, which was the vehicle for CPF. The CPF challenge groups were administered oral physiological saline, pure olive oil, or DHA (50, 100 and 400 mg/kg dosages) for 5 days. The animals were weighed on the sixth day and then administered CPF (279 mg/kg, subcutaneously). The rats were weighed again 24 h following CPF administration. The body temperatures and locomotor activities of the rats were also measured. Blood samples, brain and liver tissues were collected for biochemical, histopathological and immunohistochemical examinations. A comparison with the control group demonstrated that CPF administration increased malondialdehyde (MDA) levels in blood, brain and liver, while it reduced catalase (CAT), superoxide dismutase (SOD) and glutathione peroxidase (GPx) concentrations ( p < 0.05–0.001). Advanced oxidation protein products (AOPPs) increased only in the brain ( p < 0.001). DHA reduced these changes in MDA and AOPP values ( p < 0.05–0.001), while it increased CAT, SOD and GPx concentrations ( p < 0.05–0.001). Similarly, DHA prevented the decreases in body weight, body temperature and locomotor activities caused by CPF at 100 mg/kg and 400 mg/kg dosages ( p < 0.05–0.001). Similar to the physiological and biochemical changes, the histopathological damage scores, which increased with CPF ( p < 0.05–0.01), decreased at all three dosages of DHA ( p < 0.05–0.01). Our findings suggest that DHA, by supporting the antioxidant mechanism, reduces toxicity caused by CPF.

scholarly journals Potential protective effects of bilirubin following the treatment of neonatal hypoxic-ischemic encephalopathy with hypothermia therapy

2019 ◽  
Vol 39 (6) ◽  
Author(s):  
Liangyan Zou ◽  
Hao Yuan ◽  
Qing Liu ◽  
Chunmei Lu ◽  
Laishuan Wang
Keyword(s):  
Serum Bilirubin ◽  
Neuroprotective Effect ◽  
Controlled Trial ◽  
Hypoxic Ischemic Encephalopathy ◽  
Control Group ◽  
Protective Effects ◽  
Wide Range ◽  
Antioxidant Function ◽  
And Control ◽  
Ischemic Encephalopathy

AbstractBackground: Therapeutic hypothermia (TH) is the standard therapy for hypoxic-ischemic encephalopathy (HIE) and is associated with a wide range of physiological changes. Objective: We re-evaluated the effects of HIE and TH on bilirubin measurements following HIE in a center involved in the China cooling randomized controlled trial (RCT). Methods: Serial serum bilirubin concentrations measured during the first week of life were compared among the HIE + NT (normothermia) group, HIE + TH treatment group and control group (without HIE). Survivors of HIE were followed and assessed at approximately 2 years of age, and the results were correlated with peak bilirubin levels during the first week of life. Results: One hundred and thirty-eight infants were available for analysis. Significantly lower bilirubin levels were recorded in the HIE + NT group than in the controls (P<0.05). Significant differences were not observed among the patients in the HIE + NT group (mild to severe) or between the HIE + TH group and the HIE + NT group at any time point (P>0.05). The peak serum bilirubin concentrations recorded at 96 h of age showed a good correlation with the results of the Bayley Scales of Infant and Toddler Development, third edition (BSID-III) (P=0.02). Conclusion: Bilirubin potentially exerts a neuroprotective effect during the first week of life, and low temperature does not affect the possible antioxidant function of bilirubin during TH following HIE.

scholarly journals Serum and Brain Metabolomic Variations Reveal Perturbation of Sleep Deprivation on Rats and Ameliorate Effect of Total Ginsenoside Treatment

2017 ◽  
Vol 2017 ◽  
pp. 1-14 ◽  
Author(s):  
Xiao-jun Gou ◽  
Fang Cen ◽  
Zi-quan Fan ◽  
Ying Xu ◽  
Hong-yi Shen ◽  
...  
Keyword(s):  
Sleep Deprivation ◽  
Brain Tissue ◽  
Panax Ginseng ◽  
Well Being ◽  
Metabolite Profile ◽  
Control Group ◽  
Protective Effects ◽  
Serum Samples ◽  
Tissue Samples ◽  
And Control

Sleep loss or sleep deprivation (SD) refers to shorter sleep than average baseline need, and SD has been a serious problem of modern societies which affects health and well-being.Panax ginsengis a well-known traditional Chinese medicine (TCM). Our previous study has demonstrated that total ginsenosides (GS), the extracts fromPanax ginseng, could effectively improve cognition and behavior on SD rats. However, little is known about its metabolomic study. In this study, serum and brain metabolomic method based on gas chromatography coupled with mass spectrometry (GC/MS) was employed to evaluate the efficacy and study the mechanism of GS on a rat model of SD. With pattern recognition analysis of serum and brain tissue metabolite profile, a clear separation of the model group and control group was acquired for serum and brain tissue samples; the MGS (model + GS) group showed a tendency of recovering when compared to control group, which was consistent with behavioral and biochemical parameters. 39 and 40 potential biomarkers of brain tissues and serum samples, respectively, were identified and employed to explore the possible mechanism. Our work revealed that GS has significant protective effects on SD, and metabolomics is a useful tool for evaluating efficacy and elucidating mechanism in TCM.

scholarly journals Effect of Resveratrol on Hematological and Biochemical Alterations in Rats Exposed to Fluoride

2014 ◽  
Vol 2014 ◽  
pp. 1-5 ◽  
Author(s):  
Nurgül Atmaca ◽  
Ebru Yıldırım ◽  
Bayram Güner ◽  
Ruhi Kabakçı ◽  
Fatih Sultan Bilmen
Keyword(s):  
Enzyme Activity ◽  
Biochemical Parameters ◽  
Hematological Parameters ◽  
Treated Group ◽  
Inorganic Phosphorus ◽  
Control Group ◽  
Protective Effects ◽  
Biochemical Alterations ◽  
Fluoride Treatment ◽  
Hematological And Biochemical Parameters

We investigated the protective effects of resveratrol on hematological and biochemical changes induced by fluoride in rats. A total of 28 rats were divided into 4 groups: control, resveratrol, fluoride, and fluoride/resveratrol(n=7each), for a total of 21 days of treatment. Blood samples were taken and hematological and biochemical parameters were measured. Compared to the control group, the fluoride-treated group showed significant differences in several hematological parameters, including decreases in WBC, RBC, and PLT counts and neutrophil ratio. The group that received resveratrol alone showed a decrease in WBC count compared to the control group. Furthermore, in comparison to the control group, the fluoride group showed significantly increased ALT enzyme activity and decreased inorganic phosphorus level. The hematological and biochemical parameters in the fluoride + resveratrol treated group were similar to control group. In the fluoride + resveratrol group, resveratrol restored the changes observed following fluoride treatment, including decreased counts of WBC, RBC, and PLT, decreased neutrophil ratio and inorganic phosphorus levels, and elevated ALT enzyme activity. The present study showed that fluoride caused adverse effects in rats and that resveratrol reduced hematological and biochemical alterations produced by fluoride exposure.

scholarly journals Neuroprotective Propensity of 4-Allylpyrocatechol Derivatives against Oxaliplatin Induced Peripheral Neuropathy

2021 ◽  
pp. 92-100
Author(s):  
Tirupathi Rao Annavarapu ◽  
Sujana Kamepalli ◽  
Vijay Kotra ◽  
G. Venkata Rao
Keyword(s):  
Neuropathic Pain ◽  
Peripheral Neuropathy ◽  
Thermal Hyperalgesia ◽  
The Body ◽  
Protective Effects ◽  
Biochemical Alterations ◽  
Functional Deficits ◽  
Growing Cell ◽  
End Of Treatment ◽  
Number Of Patients

Chemotherapy is used for the treatment of rapidly growing cell diseases in the body. It is most used for the treatment of different kinds of tumors. It can develop neuropathic pain due to damage of peripheral nerve cells and it is called Chemotherapy-Induced Peripheral Neuropathy (CIPN). In this study, we have reported the protective effects of 4-allyl pyrocatechol (4-APC) and its derivatives from biochemical and functional deficits associated with oxaliplatin (OP) induced neuropathy. The animals were submitted to mechanical and thermal hyperalgesia tests, after treatment with OP three times weekly at 0.20 mg/kg and 4-APC and derivatives (10 mg/kg & 30 mg/kg). The pain parameters were evaluated during the treatment period and at the end of treatment. 4-APC significantly prevented the mice from behavioural and biochemical alterations associated with OP-induced neuropathy. Thus, we conclude from this study, the use of 4-APC and its derivatives with OP might reduce the number of patients who develop painful peripheral neuropathy.

scholarly journals Correction of ischemic optic neuropathy in rats by carbamylated darbepoetin

2018 ◽  
Vol 4 (1) ◽  
pp. 43-50 ◽  
Author(s):  
Anna A. Peresypkina
Keyword(s):  
Image Analysis ◽  
Optic Neuropathy ◽  
Neuroprotective Effect ◽  
Control Group ◽  
Ischemic Optic Neuropathy ◽  
Image Analysis System ◽  
Recombinant Erythropoietin ◽  
Protective Effects ◽  
Doppler Flowmetry ◽  
Partial Restoration

Introduction: The protective effects of carbamylated darbepoetin on the model of ischemic optic neuropathy in rats were revealed. Objectives: To increase the effectiveness of pharmacological correction of experimental ischemic optic neuropathy in an experiment by using carbamylated darbepoetin. Methods: Measuring the microcirculation level in the retina of rats was carried out using laser Doppler flowmetry (LDF). Registration was done by the data acquisition system Biopac-systems MP-150 and AcqKnowledge 4.2 programme. For microscopy and morphometry, the prepared microslides were scanned using Mirax Desk, a computerised archiving and image analysis system. Image analysis and morphometry were carried out by Pannoramic Viewer 1.15.4. Results and discussion: In the group with correction of ischemic optic neuropathy by carbamylated darbepoetin, 300 μg/kg, the microcirculation level increases by 41.9%, p&lt;0.05 in comparison with the control group, but also below the norm values by 20.3%, p&lt;0.05. In the group with correction by recombinant erythropoietin, 50 IU/kg, the microcirculation rate increases by 36.7%, p&lt;0.05 in comparison with the control group and significantly lower - by 23.3%, p&lt;0.05 - in comparison with the group of intact animals. Qualitative and quantitative morphological indices (thickness of retinal layers) helped to reveal a neuroprotective effect of carbamylated darbepoetin to a greater extent than that of recombinant erythropoietin. Conclusion: The obtained data allow drawing a conclusion about partial restoration of blood flow and preservation of neuronal retinal structures when correcting ischemic optic neuropathy in rats with carbamylated darbepoetin to a greater extent than with recombinant erythropoietin.

scholarly journals Removal of Toxic Metals from the Body of Cows by Using Antidote Substances, with its Impact on Milk Productivity and Environmental Safety of Agroecosystems around the Industrial City in Ukraine

2021 ◽  
Vol 04 (04) ◽  
pp. 154-177
Author(s):  
Serhii Portiannyk ◽  
◽  
Oleksii Mamenko ◽  
Keyword(s):  
Heavy Metals ◽  
Gastrointestinal Tract ◽  
Toxic Metals ◽  
Environmental Safety ◽  
The Body ◽  
Biologically Active ◽  
Control Group ◽  
Protective Effects ◽  
Industrial City ◽  
Experimental Group

Toxic metals like Cd and Pb pose the greatest ecological threat to ecosystems, especially in and around the industrial cities. Four farms located around Kharkiv industrial city were chosen for scientific experiments carried out on cows feeding specially developed antidote (mineral-vitamin premix "MP-A") and subcutaneous injection of biologically active preparation "BP-9". These novelty products enhanced the urinary excretion of heavy metals Cd, Pb, Cu and Zn while ensuring the production of high quality environmentally safe milk. The toxicants are absorbed from the gastrointestinal tract into the blood, travel across the body, accumulate in organs and tissues, and pass through urine and milk. The accumulation of Cd in the blood of tested cows in control group was, on average, from 77.94 to 101.20 nmol/L, and of Pb from 4.63 to 8.32 μmol/L. The transfer of Cd from blood to urine was, on average, 1.7%-2.0%, and of Pb 5.4-7.3%. The antidote substances contributed to the exacerbation of heavy metal extermination from the body of animals and the restoration of its homeostasis. The transfer of Cd from blood to urine averaged 3.9% to 9.5% and of Pb 37.7% to 103.5% in second experimental group of cows. The same for Cd was 7.1% to 12.7% and for Pb was 70.7% to 144.1% in third experimental group. The mineral-vitamin premix and biopreparation BP-9 blocked absorption of the pollutants into the gastrointestinal tract, strengthened the protective effects on organs, and facilitated the elimination of heavy metals through urine. Dairy productivity of animals also increased in cows of the second and third experimental groups by 17-22 kg per day compared to the control group having 14 kg per day (P<0.01).

scholarly journals Effect of Spermidine on Ameliorating Spermatogenic Disorders in Diabetic Mice Via Regulating Glycolysis Pathway.

2021 ◽  
Author(s):  
Jin Yuan Wang ◽  
Duo Ma ◽  
Min Luo ◽  
Yong-Peng Tan ◽  
Ou Zhong ◽  
...  
Keyword(s):  
Sperm Count ◽  
The Body ◽  
Diabetic Group ◽  
Control Group ◽  
Testis Weight ◽  
Protective Effects ◽  
Diabetic Mice ◽  
Regulated Expression ◽  
Positive Effect

Abstract Diabetes mellitus (DM), a high incidence metabolic disease, is related to the impairment of male spermatogenic function. Spermidine (SPM), one of the biogenic amines, was identified from human seminal plasma and believed to have multiple pharmacological functions. However, there exists little evidence that reported SPM's effects on moderating diabetic male spermatogenic function. Thus, the objective of this study was to investigate the SPM's protective effects on testicular spermatogenic function in streptozotocin (STZ)-induced type 1 diabetic mice. Therefore, 40 mature male C57BL/6J mice male were divided into four main groups: the control group (n=10), the diabetic group (n=10), the 2.5 mg/kg SPM-treated diabetic group (n=10) and the 5 mg/kg SPM-treated diabetic group (n=10), which was given intraperitoneally for 8 weeks. The type 1 diabetic mice model was established by a single intraperitoneal injection of STZ 120 mg/kg. The results showed that, compare to the control group, the body and testis weight, as well the number of sperm were decreased, while the rate of sperm malformation was significantly increased in STZ-induced diabetic mice. Then the testicular morphology was observed, which showed that seminiferous tubule of testis were arranged in mess, the area and diameter of which was decreased, along with downregulated anti-apoptotic factor (Bcl-2) expression, and upregulated pro-apoptotic factor (Bax) expression in the testes. Furthermore, testicular genetic expression levels of Sertoli cells (SCs) markers (WT1, GATA4) and Vimentin detected that the pathological changes aggravated observably, such as the severity of tubule degeneration increased. Compared to the saline-treated DM mice, SPM treatment markedly improved testicular function, with an increment in the body and testis weight as well as sperm count. Pro-apoptotic factor (Bax) was down-regulated expression with the up-regulated expression of Bcl-2 and suppression of apoptosis in the testes. What’s more, expression of WT1, GATA4, Vimentin and the expressions of glycolytic rate-limiting enzyme genes (HK2, PKM2, LDHA) in diabetic testes were also upregulated by SPM supplement. The evidence derived from this study indicated that the SMP's positive effect on moderating spermatogenic disorder in T1DM mice's testis. This positive effect is delivered via promoting spermatogenic cell proliferation and participating in the glycolytic pathway's activation.

Sign in / Sign up

Export Citation Format

Share Document