scholarly journals Ecological aspects and genetic diversity of Shorea balangeran in two forest types of Muara Kendawangan Nature Reserve, West Kalimantan, Indonesia

2019 ◽  
Vol 20 (2) ◽  
pp. 482-488 ◽  
Author(s):  
FITRI INDRIANI ◽  
ULFAH J SIREGAR ◽  
DEDEN D MATRA ◽  
ISKANDAR Z SIREGAR
Keyword(s):  
Genetic Diversity ◽  
Forest Restoration ◽  
Nature Reserve ◽  
Molecular Genetic ◽  
Field Research ◽  
Forest Types ◽  
Dry Land ◽  
West Kalimantan ◽  
Ecological Aspects ◽  
Wide Range

Abstract. Indriani F, Siregar U, Matra DD, Siregar IZ. 2019. Ecological aspects and genetic diversity of Shorea balangeran in two forest types of Muara Kendawang Nature Reserve, West Kalimantan, Indonesia. Biodiversitas 20: 482-488. Shorea balangeran (balangeran) belongs to the Dipterocarpaceae family that is distributed naturally in Indonesia with a wide range of ecological conditions including peat swamp and heath forests. Good adaptability of S. balangeran remains unknown concerning molecular (genetic) responses that are important to provide scientific knowledge for supporting forest restoration, especially for peatland restoration efforts. The main objective of this research was to analyze the ecological aspects and genetic diversity of S. balangeran in two types of forests, namely: peat forests (wetland) and heath forests (dry land). Field research was conducted in Muara Kendawangan Nature Reserve, West Kalimantan following standard vegetation analysis by establishing purposively sample plots (50 m x 50 m). Genetic diversity was assessed by RAPD analysis using ten random polymorphic primers. Results showed that both in heath and peat forests, S. balangeran was dominant, but only at pole and tree stages indicating that poor regeneration capacity of S. balangeran. This finding also confirmed the critically endangered status of S. balangeran as listed in the IUCN Red List of Threatened Species. Genetic analysis using RAPD did not show a clustering of S. balangeran between peat forest and heath forest populations, indicating that S. balangeran has good adaptability to grow in both forest types.

Genetic diversity in perennial ryegrass (Lolium perenne) evaluated by hybridization with ribosomal DNA: implications for cultivar identification and breeding

1998 ◽  
Vol 131 (1) ◽  
pp. 23-30 ◽  
Author(s):  
K. M. F. WARPEHA ◽  
I. CAPESIUS ◽  
T. J. GILLILAND
Keyword(s):  
Genetic Diversity ◽  
Ribosomal Dna ◽  
Perennial Ryegrass ◽  
Molecular Genetic ◽  
Genetic Research ◽  
Morphological Characteristics ◽  
Intergenic Spacer ◽  
Wide Range ◽  
Low Incidence ◽  
Potential Use

To date, little molecular genetic research has been conducted in Lolium and the current study is the first investigation of diversity in ribosomal DNA (rDNA) among perennial ryegrass cultivars. Ribosomal DNA probes derived from the intergenic spacer (IGS–M) and transcribed (COD–M) regions of mustard (Sinapis alba) were used to examine 35 perennial ryegrass (L. perenne L.) cultivars for genetic diversity using RFLP methodology. Analysis of digested genomic DNA on Southern blots revealed only 10 cultivars with unique RFLP patterns. Genetic diversity was exclusively associated with the IGS–M probe, as the cultivars were all indistinguishable when examined with the COD–M probe. This low incidence of distinctions was not expected, given that the 35 cultivars were known genetically distinct entities, representing the very wide range of the major phenotypic and physiological characteristics present in commercial stocks. Furthermore, the forage cultivars were proportionately less polymorphic in comparison to the amenity cultivars. This was also unexpected as amenity cultivars are recognized as a more closely clustered group than the forage cultivars, based on morphological characteristics. The potential use of rDNA for assessing cultivar diversity and breeding histories in perennial ryegrass is considered.

DINAMIKA POLITIK PERSETUJUAN BERSAMA TENTANG PENETAPAN DESA ADAT DI KABUPATEN ROKAN HULU TAHUN 2014

2017 ◽  
Vol 15 (1) ◽  
pp. 21
Author(s):  
Haryo Suganda ◽  
Raja Muhammad Amin
Keyword(s):  
Regulatory Region ◽  
Field Research ◽  
Analysis Techniques ◽  
Political Dynamics ◽  
Political Interests ◽  
Wide Range ◽  
Information Method ◽  
Qualitative Descriptive ◽  
The Village

This study is motivated the identification of policies issued by the regional Governmentof Rokan Hulu in the form of Regulatory region number 1 by 2015 on the determination of thevillage and Indigenous Village. Political dynamics based on various interests against themanufacture of, and decision-making in the process of formation of the corresponding localregulations determination of Indigenous Villages in the Rokan Hulu is impacted to a verysignificantamount of changes from the initial draft of the number i.e. 21 (twenty one) the villagebecame Customary 89 (eighty-nine) the Indigenous Villages who have passed. Type of thisresearch is a qualitative descriptive data analysis techniques. The research aims to describe theState of the real situation in a systematic and accurate fact analysis unit or related research, aswell as observations of the field based on the data (information). Method of data collectionwas done with interviews, documentation, and observations through fieldwork (field research).The results of the research on the process of discussion of the draft local regulations andmutual agreement about Designation of Indigenous Villages in the Rokan Hulu is, showed thatthe political dynamics that occur due to the presence of various political interests, rejectionorally by Villagers who were judged to have met the requirements of Draft Regulations to beformulated and the area for the set to be Indigenous Villages, and also there is a desire fromsome villages in the yet to Draft local regulations in order to set the Indigenous village , there isa wide range of interests of these aspects influenced the agreement to assign the entire localVillage which is in the Rokan Hulu become Indigenous village, and the village of Transmigrationinto administrative Villages where the initiator of the changes in the number of IndigenousVillages in the Rokan Hulu it is the desire of the local Government of its own.

Additions and clarifications to the flora of “Castel” nature reserve (the Southern Coast of the Crimea)

2020 ◽  
pp. 112-127
Author(s):  
L. E. Ryff
Keyword(s):  
Vascular Plants ◽  
Nature Reserve ◽  
Vascular Plant ◽  
Field Research ◽  
Southern Coast ◽  
Crimean Peninsula ◽  
Annotated List ◽  
The Crimea ◽  
Protected Natural Area ◽  
Biological Flora

The aim of the work is to supplement and clarify the annotated list of vascular plants of “Castel” nature reserve on the Southern coast of the Crimea. Methods. The work is based on the results of long standing field research, which was carried out by the traditional route-reconnaissance method, analysis of YALT herbarium materials and data from literary and Internet sources. Arealogical and biomorphological characteristics of species are given according to "Biological Flora of the Crimea" by V.N. Golubev, biotope coding according to EUNIS habitat classification. The nomenclature of taxa corresponds to the " Spontaneuos flora of the Crimean peninsula" by A.V. Yena and to international databases Euro+Med PlantBase, The Plant List, Catalog of Life. Results. An additional annotated list of vascular plants of “Castel” nature reserve has been compiled, including 152 species, subspecies and cultivars from 97 genera of 38 families, of which 53 genera and 11 families have also not been cited for this territory before. Arealogical, biomorphological, biotopic and sozological characteristics of the taxa are given. As a result of a critical analysis of the list previously published by E.S. Krainyuk, four species were redefined, two species were proposed to be excluded from the flora of the specially protected natural area, several taxa are considered doubtful. Conclusions. The list of vascular plant taxa in “Castel” nature reserve has been supplemented with 11 families, 53 genera, and 152 species, subspecies, and cultivars; several species from the previously compiled list have been proposed to be excluded or considered doubtful. Thus, the flora of the protected area includes at least 425 species from 68 families. For the first time, the biotopic characteristic of the flora of the reserve was established.

scholarly journals Whole genome-based characterisation of antimicrobial resistance and genetic diversity in Campylobacter jejuni and Campylobacter coli from ruminants

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Medelin Ocejo ◽  
Beatriz Oporto ◽  
José Luis Lavín ◽  
Ana Hurtado
Keyword(s):  
Genetic Diversity ◽  
Antimicrobial Resistance ◽  
Campylobacter Jejuni ◽  
Resistance Genes ◽  
Campylobacter Coli ◽  
Whole Genome ◽  
Northern Spain ◽  
Sequence Alignments ◽  
Phenotypic Data ◽  
Wide Range

AbstractCampylobacter, a leading cause of gastroenteritis in humans, asymptomatically colonises the intestinal tract of a wide range of animals.Although antimicrobial treatment is restricted to severe cases, the increase of antimicrobial resistance (AMR) is a concern. Considering the significant contribution of ruminants as reservoirs of resistant Campylobacter, Illumina whole-genome sequencing was used to characterise the mechanisms of AMR in Campylobacter jejuni and Campylobacter coli recovered from beef cattle, dairy cattle, and sheep in northern Spain. Genome analysis showed extensive genetic diversity that clearly separated both species. Resistance genotypes were identified by screening assembled sequences with BLASTn and ABRicate, and additional sequence alignments were performed to search for frameshift mutations and gene modifications. A high correlation was observed between phenotypic resistance to a given antimicrobial and the presence of the corresponding known resistance genes. Detailed sequence analysis allowed us to detect the recently described mosaic tet(O/M/O) gene in one C. coli, describe possible new alleles of blaOXA-61-like genes, and decipher the genetic context of aminoglycoside resistance genes, as well as the plasmid/chromosomal location of the different AMR genes and their implication for resistance spread. Updated resistance gene databases and detailed analysis of the matched open reading frames are needed to avoid errors when using WGS-based analysis pipelines for AMR detection in the absence of phenotypic data.

scholarly journals Phenotypic Characterisation for Growth and Nut Characteristics Revealed the Extent of Genetic Diversity in Wild Macadamia Germplasm

Agriculture ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 680
Author(s):  
Thuy T. P. Mai ◽  
Craig M. Hardner ◽  
Mobashwer M. Alam ◽  
Robert J. Henry ◽  
Bruce L. Topp
Keyword(s):  
Genetic Diversity ◽  
Growth Traits ◽  
Principal Component ◽  
Conservation Strategies ◽  
Wild Germplasm ◽  
Phenotypic Characterisation ◽  
Wide Range ◽  
Genetic And Environmental Factors ◽  
Positive Correlations ◽  
Wild Accessions

Macadamia is a recently domesticated Australian native nut crop, and a large proportion of its wild germplasm is unexploited. Aiming to explore the existing diversity, 247 wild accessions from four species and inter-specific hybrids were phenotyped. A wide range of variation was found in growth and nut traits. Broad-sense heritability of traits were moderate (0.43–0.64), which suggested that both genetic and environmental factors are equally important for the variability of the traits. Correlations among the growth traits were significantly positive (0.49–0.76). There were significant positive correlations among the nut traits except for kernel recovery. The association between kernel recovery and shell thickness was highly significant and negative. Principal component analysis of the traits separated representative species groups. Accessions from Macadamia integrifolia Maiden and Betche, M. tetraphylla L.A.S. Johnson, and admixtures were clustered into one group and those of M. ternifolia F. Muell were separated into another group. In both M. integrifolia and M. tetraphylla groups, variation within site was greater than across sites, which suggested that the conservation strategies should concentrate on increased sampling within sites to capture wide genetic diversity. This study provides a background on the utilisation of wild germplasm as a genetic resource to be used in breeding programs and the direction for gene pool conservation.

Molecular markers and antioxidant activity in berry crops: Genetic diversity analysis

2012 ◽  
Vol 92 (6) ◽  
pp. 1121-1133 ◽  
Author(s):  
S. C. Debnath ◽  
Y. L. Siow ◽  
J. Petkau ◽  
D. An ◽  
N. V. Bykova
Keyword(s):  
Genetic Diversity ◽  
Antioxidant Activity ◽  
Molecular Markers ◽  
Molecular Techniques ◽  
Diversity Analysis ◽  
Full Potential ◽  
Genetic Diversity Analysis ◽  
Wide Range ◽  
Berry Crops ◽  
Improvement Programs

Debnath, S. C., Siow, Y. L., Petkau, J., An, D. and Bykova, N. V. 2012. Molecular markers and antioxidant activity in berry crops: Genetic diversity analysis. Can. J. Plant Sci. 92: 1121–1133. An improved understanding of important roles of dietary fruits in maintaining human health has led to a dramatic increase of global berry crop production. Berry fruits contain relatively high levels of vitamin C, cellulose and pectin, and produce anthocyanins, which have important therapeutic values, including antitumor, antiulcer, antioxidant and anti-inflammatory activities. There is a need to develop reliable methods to identify berry germplasm and assess genetic diversity/relatedness for dietary properties in berry genotypes for practical breeding purposes through genotype selection in a breeding program for cultivar development, and proprietary-rights protection. The introduction of molecular biology techniques, such as DNA-based markers, allows direct comparison of different genetic materials independent of environmental influences. Significant progress has been made in diversity analysis of wild cranberry, lowbush blueberry, lingonberry and cloudberry germplasm, and in strawberry and raspberry cultivars and advanced breeding lines developed in Canada. Inter simple sequence repeat (ISSR) markers detected an adequate degree of polymorphism to differentiate among berry genotypes, making this technology valuable for cultivar identification and for the more efficient choice of parents in the current berry improvement programs. Although multiple factors affect antioxidant activity, a wide range of genetic diversity has been reported in wild and cultivated berry crops. Diversity analysis based on molecular markers did not agree with those from antioxidant activity. The paper also discusses the issues that still need to be addressed to utilize the full potential of molecular techniques including expressed sequence tag-polymerase chain reaction (EST-PCR) analysis to develop improved environment-friendly berry cultivars suited to the changing needs of growers and consumers.

Genetic Diversity Analysis of Salvadora Oleoides Decne: An Evergreen Dry Land Fruit Tree of Rajasthan, India

2021 ◽  
Author(s):  
Juleri M Upendra ◽  
Shari Nair ◽  
Satyawada R Rao ◽  
Harchand R Dagla
Keyword(s):  
Genetic Diversity ◽  
Random Amplified Polymorphic Dna ◽  
Splice Junction ◽  
Genetic Distances ◽  
Climatic Conditions ◽  
Fruit Tree ◽  
Group Method ◽  
Dry Land ◽  
Intron Splice ◽  
Semi Arid

Abstract Genetic diversity of Salvadora oleoides Decne is analyzed by cumulative data of 10 Random Amplified Polymorphic DNA (RAPD), 10 Inter Simple Sequence Repeats (ISSR) and 7 Intron Splice Junction (ISJ) markers. The plant is an evergreen fruit tree and well distributed in semi-arid and sub-humid climatic conditions of Rajasthan, India. RAPD, ISSR and ISJ primers accounted for 84.4%, 85.3%, 85.9% polymorphism. Average 0.23 PIC is accounted for RAPD, ISSR and ISJ primers. The genetic similarity ranged between 0.42-0.89. Analysis of molecular variance (AMOVA) revealed higher variation (73%) at intra-population than inter-population (27%) level. Genetic distances based on Un-weighted Pair Group Method with Arithmetic mean (UPGMA) dendrogram and Principal Coordinate Analysis (PCoA) is correlated with physical distances or climatic conditions of Salvadora oleoides Decne in a semi-arid and sub-humid environment of Rajasthan. The present investigation may help in the understanding of gene flow systems between physical distances and environmental heterogeneity of the populations for better management of Salvadora oleoides Decne in the region.

Epigenetics and Applied Anthropology

2021 ◽  
Author(s):  
Charles H. Klein
Keyword(s):  
Social Inequalities ◽  
Homo Sapiens ◽  
Single Gene ◽  
Molecular Genetic ◽  
Healthcare Providers ◽  
Basic Research ◽  
Sufficient Information ◽  
Theoretical Frameworks ◽  
Noncoding Dna ◽  
Wide Range

Since Francis Crick and James D. Watson’s discovery of DNA in 1953, researchers, policymakers, and the general public have sought to understand the ways in which genetics shapes human lives. A milestone in these efforts was the completion of the Human Genome Project’s (HGP) sequencing of Homo sapiens’ nearly three million base pairs in 2003. Yet, despite the excitement surrounding the HGP and the discovery of the structural genetic underpinnings of several debilitating diseases, the vast majority of human health outcomes have not been linked to a single gene. Moreover, even when genes have been associated with particular diseases (e.g., breast and colon cancer), it is not well understood why certain genetically predisposed individuals become ill and others do not. Nor has the HGP’s map provided sufficient information to understand the actual functioning of the human genetic code, including the role of noncoding DNA (“junk DNA”) in regulating molecular genetic processes. In response, a growing number of scientists have shifted their attention from structural genetics to epigenetics, the study of how genes express themselves in particular situations and environments. Anthropologists play roles in these applications of epigenetics to real-world settings. Their new theoretical frameworks unsettle the nature-versus-nurture binary and support biocultural anthropological research demonstrating how race becomes biology and embodies social inequalities and health disparities across generations. Ethnographically grounded case studies further highlight the diverse epigenetic logics held by healthcare providers, researchers, and patient communities and how these translations of scientific knowledge shape medical practice and basic research. The growing field of environmental epigenetics also offers a wide range of options for students and practitioners interested in applying the anthropological toolkit in epigenetics-related work.

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