Methionine supplementing effects on intestine, liver and uterus morphology, and on positivity and expression of Calbindin-D28k and TRPV6 epithelial calcium carriers in laying quail in thermoneutral conditions and under thermal stress

This study aimed to provide the performance, localization and expression of the epithelial calcium transporter channels Calbindin-D28k (Calb) and TRPV6, and of the morphology of the digestive and reproductive system of laying quail under heat stress (HS), and with methionine supplementation (MS). This study characterized the positivity (immunohistochemistry) and expression (real-time PCR) of calcium channels in the kidneys, intestine and uterus of 504 laying quails under different MS (100, 110 and 120%) and temperatures (20, 24, 28 and 32°C). The animals under HS (32°C) had lower villus height, villus:crypt ratio, and goblet cell index in the duodenum and jejunum, fewer secondary and tertiary uterine folds, smaller hepatic steatosis, and increased number of distal convoluted renal tubules (CT) positive to Calb, and increased positivity in proximal CTs. Deleterious effects of HS were minimized with MS for: duodenal crypts, number of goblet cells of the jejunum, number of uterine folds, decreased Calb positivity in intestines and kidney, increased positivity of Calb in the uterus and increased TRPV6 gene expression in the kidney (P≤0.05). Epithelial calcium transporters were altered due to less need for calcium absorption and reabsorption due to more calcium available with the MS, increasing egg production in HS and quality in termoneutrality (P≤0.05). MS further increased intestinal villus absorption area and height, increased steatosis, decreased Calb positivity in the intestine and kidney, increased uterine positivity of Calb, and increase Calb and TRPV6 expression in the kidney (P≤0.001) under thermoneutrality. It was concluded that the use of MS (120%) is justifiable in order to partially reverse the deleterious effects of HS on the production, in the epithelial calcium carriers, and in the digestory and reproductive morphology of laying quail.

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Effects of methionine supplementing on intestine, liver and uterus morphology, and on positivity and expression of calbindin-D28k and TRPV6 calcium carriers in laying quails in thermoneutral conditions and under thermal stress

10.1101/2020.01.23.916551 ◽

2020 ◽

Author(s):

Lanuza Ribeiro de Moraes ◽

Maria Eduarda Araújo Delicato ◽

André da Silva Cruz ◽

Hugo Thyares Fonseca Nascimento Per da Silva ◽

Clara Virgínia Batista de Vasconcelos Alves ◽

...

Keyword(s):

Heat Stress ◽

Thermal Stress ◽

Egg Production ◽

Calcium Absorption ◽

Renal Tubules ◽

Intestinal Villus ◽

Absorption Area ◽

Gene And Protein Expression ◽

Calbindin D28k ◽

Methionine Supplementation

AbstractThe aim of this study was to provide support for the performance, localization and expression of the epithelial calcium transporter channels, calbindin-D28k (Calb) and TRPV6, and of the morphology of the digestive and reproductive system of laying quails under heat stress, and with methionine supplementation. Therefore, the present study characterized the positivity (immunohistochemistry) and expression (real-time PCR) of calcium channels (Calb and TRPV6) in the kidneys, intestine and uterus of 504 laying quails that were submitted to different methionine supplementation (100, 110 and 120%) and temperatures (20, 24, 28 and 32°C). The animals under thermal stress had lower villus height, villus:crypt ratio, and goblet cell index in the duodenum and jejunum, fewer secondary and tertiary uterine folds, smaller hepatic steatosis, and increased number of distal convoluted renal tubules (CT) positive to Calb (protein), and increased positivity in proximal CTs. The deleterious effects of heat stress were minimized with methionine supplementation for the following variables: duodenal crypts, number of goblet cells of the jejunum, number of uterine folds, decreased Calb positivity in intestines and kidney, increased positivity of Calb in the uterus and increased TRPV6 gene expression in the kidney. Calcium transporters were altered due to less need for calcium absorption and reabsorption due to more calcium available with the supplementation, increasing egg production and quality. Methionine supplementation further increased intestinal villus absorption area and height, increased steatosis, decreased Calb positivity in the intestine and kidney, increased uterine positivity and Calb expression, and increased TRPV6 expression in the uterus under thermoneutrality. This is the first study that describes the gene and protein expression of calcium transporters in the intestine, kidney and uterus of laying quails, and concludes that the use of methionine supplementation is justifiable in order to partially reverse the deleterious effects of thermal stress on the production.

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Penggunaan Tepung Limbah Udang sebagai Bahan Pakan Sumber Protein terhadap Performa Produksi Puyuh Fase Layer (Coturnix-coturnix japonica)

Jurnal Ilmu dan Teknologi Peternakan Tropis ◽

10.33772/jitro.v5i3.4733 ◽

2018 ◽

Vol 5 (2) ◽

pp. 31

Author(s):

Hamdan Has ◽

Astriana Napirah ◽

Widhi Kurniawan ◽

Natsir Sandiah

Keyword(s):

Egg Production ◽

Production Performance ◽

Feed Consumption ◽

Shrimp Waste ◽

Feed Conversion ◽

Treatment Unit ◽

Egg Weight ◽

Randomized Design ◽

Average Production ◽

Laying Quail

ABSTRAKLimbah udang merupakan limbah pengolahan udang yang memiliki potensi sebagai pakan sumber protein bagi ternak puyuh. Penelitian ini bertujuan mengkaji penggunaan tepung limbah udang (TLU) sebagai sumber protein pakan pada puyuh fase layer. Penelitian ini menggunakan rancangan acak lengkap empat perlakuan lima ulangan perlakuan yang digunakan terdiri dari empat level penggunaan tepung limbah udang dalam ransum yaitu P0 (kontrol), P1 (5% TLU), P2 (7,5% TLU) dan P3 (10% TLU), tiap unit perlakuan disi dengan 5 ekor puyuh. Puyuh yang diguanakan adalah puyuh fase layer umur 20 minggu, sebanyak 100 ekor yang didistribusikan kedalam 20 unit percobaan. Bahan pakan yang digunakan adalah jagung, dedak padi, konsentrat petelur dan tepung limbah udang. Data yang diperoleh dianalisis ragam dan data yang berbeda nyata (P<0,05) diuji lanjut menggunakan uji duncan. Variabel yang diamati adalah performa produksi: konsumsi pakan, produksi telur, bobot telur dan konversi ransum. Hasil penelitian menunjukkan bahwa penggunaan TLU dalam ransum (P1,P2 dan P3) menunjukkan pengaruh nyata (P<0,05) dibanding kontrol pada minggu ke-tiga penelitian terhadap bobot telur dan konversi ransum, penggunaan TLU (P1,P2,P3) selama lima minggu meningkatkan konsumsi ransum (P<0,05) dibanding kontrol tetapi tidak berbeda nyata (P>0,05) terhadap produksi telur, bobot telur dan konversi ransum. Kesimpulan penelitian ini bahwa penggunaan TLU dalam ransum dapat digunakan hingga level 7,5% sedangkan level 10% menunjukkan adanya penurunan rata-rata performa produksi.Kata kunci: tepung limbah udang, puyuh fase layer, performa produksiABSTRACTShrimp waste was shrimp processing waste which has the potential as protein source for quail feed. This study was aimed to examine the use of shrimp waste flour (SWF) asprotein source for laying quail feed. This study used completely randomized design that consist of four treatments and five replications.The treatmentswere using levels of shrimp waste flour in feed and consist of P0 (control), P1 (5% SWF), P2 (7.5% SWF) and P3 (10% SWF ). Each treatment unit was filled with 5 quails. One hundred of 20 weeks laying quails were used in this study. Self mixing feed that contained corn, rice bran, laying concentrate and shrimp waste flour were used in this study. The data obtained were analyzed using analyze of variance and continued using Duncan multiple range test. The variables observed were production performance that consist of feed consumption, egg production, egg weight and feed conversionratio. The results showed that the use of SWF in feed (P1, P2 and P3) showed a significant effect (P <0.05) compared to controls in the third week of research on egg weight and feed conversion ratio.The use of SWF (P1, P2, P3) for five weeks increased feed consumption (P <0.05) compared to controls but not significantly different (P>0.05) for egg production, egg weight and feed conversion. The conclusion of this study was the use of SWF in feed can be used until 7.5% on laying quail feed while the level of 10% indicates a decrease in average production performance.Keywords: shrimp waste flour, laying quail, production performance

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Prolonged Dark Time Within A 24-h Light Regime Improves Eggshell Quality by Enhancing Calcium Deposition in Laying Hens

10.21203/rs.3.rs-69315/v1 ◽

2020 ◽

Author(s):

Qian Xin ◽

Jingpeng Zhao ◽

Hongchao Jiao ◽

Haifang Li ◽

Xiaojuan Wang ◽

...

Keyword(s):

Feed Efficiency ◽

Egg Production ◽

Laying Hens ◽

Light Regime ◽

Calcium Deposition ◽

Eggshell Quality ◽

Dark Time ◽

Calbindin D28k ◽

Calcium And Phosphorus ◽

Calcium Transporter

Abstract Background: The absorption and transportation of calcium and phosphorus is mainly relied on their corresponding transporters. Eggshell is mainly formed during dark time in one egg cycle. The aim of this study was to determine the effect of different light regime on eggshell quality and the expression of the relevant calcium and phosphorus transporters in laying hens. Seventy two 56-week-old laying hens were randomly divided into two groups and subjected to the following treatments: 16 h light: 8 h dark (control) and 9 h light: 15 h dark (LDP). The expression of phosphorus transporters type IIb Na/Pi co-transporter NaPi-IIa (NPt2a) and NaPi-IIb (NPt2b), calcium transporter calbindin-D28k (CaBP-D28k), and plasma membrane Ca ATPase 1b (PMCA1b) were measured in small intestine, kidney, and eggshell gland. Results: The results showed that the feed intake (P < 0.001) and egg weight (P = 0.05) was decreased by LDP treatment, while laying rate, egg production, and feed efficiency were not significantly influenced (P > 0.05). Compared to control, eggshell hardness was increased (P < 0.05) by LDP treatment whereas eggshell thickness and eggshell percentage were not significant changed. Eggshell calcium (Ca) and phosphorus (P) contents were elevated in LDP-hens, compared to control birds. Compared to control birds, serum Ca (P < 0.01) and P levels (P = 0.079) at dark time were increased in LDP-hens while and alkaline phosphatase (ALP) activity was lowered (P < 0.05). The protein expression levels of CaBP-D28k and PMCA1b were not influenced in duodenum but were decreased at light time in jejunum of LDP hens. In kidney, the expression of CaBP-D28k, PMCA1b and NPt2a were not changed by LDP treatment. In eggshell gland, however, the expression of CaBP-D28k and osteopontin (OPN) were relative higher in LDP hens compared to control birds, whereas the PMCA1b expression was not altered. Conclusions: The result indicates that the increased circulating Ca and P concentrations in dark time are favorable for the deposition of calcium and phosphorus in eggshell. The result offers an alternative strategy for the laying hen with a worse eggshell problem.

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Effect of replacement different levels of sesame meal as a protein source to replace soybean meal on the productive performance and reproductive of laying quail: تأثير استبدال مستويات مختلفة من كسبة السمسم كمصدر بروتيني محل كسبة فول الصويا على الأداء الإنتاجي والتناسلي لطائر السمان البياض

Journal of agricultural, environmental and veterinary sciences - مجلة العلوم الزراعية والبيئية والبيطرية ◽

10.26389/ajsrp.r190421 ◽

2021 ◽

Vol 5 (3) ◽

pp. 70-58

Author(s):

Duraid Thonnon Younis, Khalid Hadi Mustafa Al-Sofee Duraid Thonnon Younis, Khalid Hadi Mustafa Al-Sofee

Keyword(s):

Soybean Meal ◽

Feed Intake ◽

Egg Production ◽

Protein Source ◽

Egg Mass ◽

Average Weight ◽

Productive Performance ◽

Sesame Meal ◽

Laying Quail ◽

Different Levels

The study was conducted in the poultry field in the Animal Production Department/ College of Agriculture and Forestry/ University of Mosul, for the period from 18/ 2/ 2020 until 13/ 4/ 2020. The experiment aimed to study the effect of replacement different levels of sesame meal (SSM) as a protein source to replace soybean meal (SBM) on the productive performance and reproductive of laying quail, 360 quail birds (240 females and 120 males) were used, at the age of 49 days, distributed randomly into five treatments, each treatment with eight replicates, ( 9 birds in each replicate 6 females and 3 males). The treatments were as follows: the first was the control (0% SSM), the second, third, fourth, and the fifth replaced SSM by SBM with level (25, 50, 75 and 100%) respectively, feed and water was ad libitum during the trial period of 8 weeks. The results of the statistical analysis showed there is a significant statistical difference at a level (P≤0.05) in the fifth treatment (100% SSM) as compared to control and other treatments in total egg number, average egg weight, total egg mass, Hen day egg production percentage (% HD), feed intake, feed conversion ratio, fertility, and hatching percentage of total eggs, average weight of hatched chicks, and a significant increase in the embryo mortality. The results also showed a significant decrease at a level (P≤0.05) in the feed intake for the fourth treatment (75% SSM) compared to the control, while no significant differences were observed for the other treatments in all the studied traits. The results of this study showed the possibility of using sesame meal as a protein source at (75%) to replace soybean meal in laying quail bird diets.

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High protein wheats for poultry. 3. Use in diets for laying hens

Australian Journal of Experimental Agriculture ◽

10.1071/ea9710629 ◽

1971 ◽

Vol 11 (53) ◽

pp. 629 ◽

Cited By ~ 3

Author(s):

W Turner ◽

CG Payne

Keyword(s):

Amino Acids ◽

Protein Content ◽

Egg Production ◽

Laying Hens ◽

Control Diet ◽

High Protein ◽

Well Production ◽

Meat Meal ◽

Methionine Supplementation

Two experiments were conducted to investigate the usefulness of feeding layers diets based on high protein wheats. The test diets contained about 86 per cent of high protein wheat and were supplemented with various amino acids. A control diet contained wheat of normal protein content, soyabean and meat meal. In the first experiment lysine addition to the high protein wheat diet increased egg numbers ; and methionine supplementation, in addition to lysine, tended to further improve egg production. In the second experiment lysine addition again improved egg production, but, if inethionine was added as well, production fell. The varying response can be explained by different methionine contents of the two high protein wheats used.

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Navy beans (Phaseolus vulgaris) as a protein source in layer diets

Australian Journal of Experimental Agriculture ◽

10.1071/ea9710387 ◽

1971 ◽

Vol 11 (51) ◽

pp. 387 ◽

Cited By ~ 3

Author(s):

JK Connor ◽

AR Neill ◽

HW Burton

Keyword(s):

Body Weight ◽

Phaseolus Vulgaris ◽

Egg Production ◽

Body Weight Gain ◽

Metabolizable Energy ◽

Feed Consumption ◽

Egg Mass ◽

Navy Bean ◽

Bean Meal ◽

Methionine Supplementation

The effects of raw versus steamed navy bean (Phaseolus vulgaris) meal, in diets with and without methionine supplementation, on egg production, egg weight, body weight, pancreas weight, mortality, feed consumption and conversion, and energy and nitrogen utilization were determined with laying hens. As the level of raw navy bean meal in the diet increased, egg mass production and body weight gain decreased. Declines in production were largely overcome by steaming the bean meal. Methionine supplementation of both raw and steamed meal diets had no effect on performance. Changes in dietary metabolizable energy and nitrogen retention are discussed in relation to bird performance.

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Histochemical reactivity of peanut lectin-horseradish peroxidase conjugate.

Journal of Histochemistry & Cytochemistry ◽

10.1177/28.9.7410818 ◽

1980 ◽

Vol 28 (9) ◽

pp. 979-990 ◽

Cited By ~ 117

Author(s):

P J Stoward ◽

S S Spicer ◽

R L Miller

Keyword(s):

Sialic Acid ◽

Horseradish Peroxidase ◽

Periodic Acid ◽

Apical Cell ◽

Goblet Cells ◽

Surface Epithelium ◽

Renal Tubules ◽

Peanut Lectin ◽

Periodic Acid Schiff ◽

Terminal Galactose

A peanut lectin-horseradish peroxidase (PL-HRP) conjugate has been applied to histochemical staining of paraffin sections of various mouse organs. The PL-HRP conjugate has selectively reacted with secretory bodies, the Golgi zone, and the apical cell surface in various cell types. Some positive sites, including lingual and tracheal serous glands, Brunner's glands, and the brush border of the proximal straight nephron, contained periodic acid-Schiff (PAS)-positive glycoconjugate with no affinity for basic reagents. The stored secretion in these sites was interpreted as containing neutral glycoprotein with terminal galactose residues which could, in part at least, account for the PAS reactivity. Duodenal goblet cells, which exhibited basophilia attributable to sulfate esters, also bound PL-HRP. As the binding was affected by prior sialidase digestion, the secretory glycoprotein in the duodenal goblet cells was judged to contain oligosaccharides with sulfate esters and terminal galactose uncapped by sialic acid. All sites known from their basophilia to form sialomucin failed to stain with the PL-HRP conjugate, but consistently gained reactivity following sialidase digestion and were inferred, therefore, to possess glycoproteins with oligosaccharide side chains containing subterminal galactose and terminal sialic acid. Lingual mucous glands, known to secrete a mucosubstance with basophilic properties indicative of the presence of sulfate esters but not sialic acid, stained with PL-HRP only after sialidase digestion and, accordingly, were reinterpreted as containing both sulfate esters and terminal galactose-sialic acid dimers. Staining of gastric surface epithelium demonstrated a srongly PAS-reactive neutral glycoprotein, and that of goblet cells in the cecum disclosed PAS-positive sulfated glycoprotein. The latter two sites lacked PL-HRP affinity without or with prior sialidase treatment and apparently possessed neither terminal galactose residues nor galactose-sialic acid dimers. PL-HRP affinity was observed exclusively in the Golgi cisternae of some epithelial cells, thus indicating that galactose occurs transiently as a terminal residue in this site. A few histologic sites, such as pancreatic and gastric zymogen cells and renal tubules, were devoid of both PAS reactivity and basophilia indicative of the presence of complex carbohydrate but stained strongly with the PL-HRP conjugate by means which are not understood. Galactose in the PL-HRP solution blocked or reversed the PL-HRP binding in most of the structures with an affinity for the conjugate, supporting the conclusions that the reagent is specific for galactosyl residues.

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USE OF FABABEANS IN DIETS FOR LAYING HENS

Canadian Journal of Animal Science ◽

10.4141/cjas80-049 ◽

1980 ◽

Vol 60 (2) ◽

pp. 395-405 ◽

Cited By ~ 15

Author(s):

L. D. CAMPBELL ◽

G. OLABORO ◽

R. R. MARQUARDT ◽

D. WADDELL

Keyword(s):

Heat Treatment ◽

Egg Production ◽

Egg Size ◽

Laying Hens ◽

Productive Performance ◽

Depressing Effect ◽

Feeding Trials ◽

Methionine Supplementation ◽

Series Of Experiments ◽

Supplemental Protein

A series of experiments were conducted to evaluate fababeans as a source of supplemental protein for use in diets for laying hens. Experimental and commercial-type feeding trials were used to determine the influence of level of dietary addition of fababeans, methionine supplementation, heat treatment of fababeans and dehulling of fababeans on productive performance. When diets adequately supplemented with methionine were used, egg production rates of hens fed fababeans were similar to those of controls except for high dietary levels of fababeans (in excess of 25%) where a depression was indicated. Mortality rates were not influenced by addition of fababeans to the diet. Hens fed fababeans consistently laid eggs of a smaller size than those of controls and the extent of the egg size depression was related to the level of fababeans in the diet. The egg size depressing effect of fababeans was not related to dietary levels of protein or energy and was not influenced by heat treatment of fababeans or removal of hulls from fababeans. Fababeans can be used effectively as a protein source in diets for laying hens and the effect on egg size should be a factor given consideration when the choice to use fababeans is made.

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Sodium requirement of japanese laying quail

Research Society and Development ◽

10.33448/rsd-v9i6.2486 ◽

2020 ◽

Vol 9 (6) ◽

pp. e95962486

Author(s):

Heder José D’Avila Lima ◽

Marcos Vinícius Martins Morais ◽

Sergio Luiz de Toledo Barreto

Keyword(s):

Feed Intake ◽

Egg Production ◽

The Body ◽

Relative Mass ◽

Egg Mass ◽

Average Weight ◽

Electrolyte Balance ◽

Feed Conversion ◽

Japanese Quails ◽

Laying Quail

A study was conducted in order to estimate the sodium requirements of Japanese laying quail (Coturnix coturnix japonica). In this study, we used 280 Japanese quails at 128 days of age, with an average weight of 9 ± 5.2 g, and an egg production rate of 82.3 ± 0.94%. An experimental design methodology was employed, completely randomized with five treatments and seven replications. Each replicate consisted of eight quails, with a total of thirty-five experimental units. The experimental diets were based on corn and soybean meal, and contained five different levels of sodium (0.08%, 0.12%, 0.16%, 0.20% and 0.24%). The diets were isocaloric and contained equal concentrations of amino acids, with an electrolyte balance of 250 mEq/kg. The parameters we evaluated were feed intake (g/quail/day); egg production per quail per day (%); egg weight (g); egg mass (g/quail/day); and feed conversion by egg mass (kg/kg) and per dozen eggs (kg/dz). In addition, we evaluated the composition of the eggs in terms of the absolute (g) and relative (%) mass of the yolk, albumen and eggshell. We also measured the body weight variation (g) and viability (%) of the quails. There were no significant effects (p>0.05) on the described parameters. No nutritional disorders occurred related to the level of inclusion of sodium in the diets. The inclusion of 0.08% sodium in the diet of laying Japanese quails was satisfactory to maintain egg production and quality without losses in feed intake or feed conversion.

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Impact of chlorine dioxide as water acidifying agent on the performance, ileal microflora and intestinal histology in quails

Archives Animal Breeding ◽

10.7482/0003-9438-57-031 ◽

2014 ◽

Vol 57 (1) ◽

pp. 1-9 ◽

Cited By ~ 2

Author(s):

Asad Sultan ◽

Irshad Ullah ◽

Sarzamin Khan ◽

Rifat Ullah Khan ◽

Zahoor ul Hassan

Keyword(s):

Chlorine Dioxide ◽

Microbial Population ◽

Goblet Cells ◽

Conversion Ratio ◽

Heart Weight ◽

Feed Conversion ◽

Intestinal Histology ◽

Villus Height ◽

E Coli ◽

Different Levels

Abstract. The present study was planned to investigate the effect of different levels of chlorine dioxide on the performance, gut microbiota and intestinal histology of quails. For this purpose, 300 day-old healthy quail chicks were randomly placed in 20 specially designed pens (15 birds/pen) with water troughs containing chlorine dioxide at the level of 0.00 (DW-0.00), 0.3 (DW-0.3), 0.4 (DW-0.4) and 0.5 ppm (DW-0.5) in replicated fashion (5 replicate/treatment) for 28 days. Weight gain, feed conversion ratio and dressing percentage increased significantly (P<0.05) in DW-0.5 group. Similarly, liver, gizzard and heart weight increased significantly in treated groups linearly with increasing levels of treatment at day 21 and 28. The results showed that population of Salmonella and E. coli decreased linearly at day 21 and 28 of age. Villus height and goblet cells at day 21 and 28 were significantly higher in DW-0.5 group. The results of the present study suggested that the treatment of chlorine dioxide linearly increased the performance and gut morphology and decreased microbial population in quails.

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