scholarly journals Produksi Bioetanol Dari Rumput Laut dan Limbah Agar Gracilaria sp. dengan Metode Sakarifikasi Yang Berbeda

2014 ◽  
Vol 16 (2) ◽  
pp. 65 ◽  
Author(s):  
Saniha Adini ◽  
Endang Kusdiyantini ◽  
Anto Budiharjo
Keyword(s):  
Ethanol Production ◽  
Cell Number ◽  
Reducing Sugar ◽  
Bioethanol Production ◽  
Enzymatic Process ◽  
Anova Analysis ◽  
High Demand ◽  
Variable Reduction ◽  
The Difference ◽  
Main Component

The Indonesia needs of Bioethanol were 390.000 kL in 2012, but the local ethanol production only able to cover less than 4% from the needed. The high demand of the bioethanol encourage for another innovation in ethanol production more efficient and effectively. Seaweeds and the residual pulp of Gracilaria sp. could be useful as substrate for bioethanol production, because of the high amount of polysaccharide, cellulose and galactan type. Unfortunately, this cellulose and galactan had through the saccharification process first, before they can be used as substrates in bioethanol production. This study examined the difference between two saccharification process which are acid hydrolisis using H2SO4 1% and enzymatic process using Aspergillus niger on the use seaweed and the residual pulp of Gracilaria sp. for bioethanol production. Bioethanol production been conducted for 5 days and in each 24 hour, the sampling for cell number variable, reduction sugar amount variable, and medium fermentation pH variable had been retrieved. The ethanol amount calculation in the last incubation phase conducted using distillate fermentation spesific gravity methode. The highest ethanol was obtained 5,50%  by treatment using seaweed medium with acid hydrolisis. The anova analysis result showed that interaction between medium variable and hydrolisis didn’t have signifficant influence toward ethanol product. It showed that seaweed and the residual pulp of Gracilaria sp. had same quality and they can be useful as main component of bioethanol production which are hydrolisis by enzymatic or acid hydrolisis.   Key Words :  Gracilaria sp., the residual pulp, saccharification, reducing sugar, ethanol

Bioethanol Production from Pineapple Peel Juice Using Saccharomyces cerevisiae

2014 ◽  
Vol 875-877 ◽  
pp. 242-245
Author(s):  
Jutarut Pornpunyapat ◽  
Wilaiwan Chotigeat ◽  
Pakamas Chetpattananondh
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Ethanol Production ◽  
Ethanol Concentration ◽  
Renewable Resource ◽  
Reducing Sugar ◽  
Total Sugar ◽  
Bioethanol Production ◽  
Pineapple Peel ◽  
Initial Ph ◽  
Production Characteristics

Bioethanol is widely used as renewable resource due to its safe to produce and environmentally friendly. However, knowledge on ethanol production from pineapple peel juice (Pattawia spp) is far from sufficient. In this work, pineapple peel juice (initial pH at 5) was fermented at various yeast contents (1, 3 and 5% by wt.) and fermentation times (3, 5 and 7 days) in order to investigate ethanol production characteristics. Yeast, Sacchromyces cerevisiae was grown on pineapple peel juice. The squeezed juice contained 11% of total sugar and 5% of reducing sugar. The results indicated that the optimum ethanol production was yeast contents of 5% by wt. and fermentation times of 5 days which gave the ethanol production of 9.08g/l. The ethanol at a higher yeast content also had a higher ethanol concentration.

scholarly journals Bioetanol dari Kulit Pisang (Musa paradisiaca L.) dengan Sakarifikasi dan Fermentasi Serentak

2019 ◽  
Vol 6 (1) ◽  
pp. 106
Author(s):  
I Ketut Muksin ◽  
Ni Luh Arpiwi
Keyword(s):  
Sugar Content ◽  
Pairwise Comparison ◽  
Reducing Sugar ◽  
Banana Peel ◽  
Anova Analysis ◽  
Citrate Buffer ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
The Difference ◽  
Simultaneous Saccharification

Bioethanol is one of the energy alternatives which environmentally friendly and sustainable. This research employed Completely Randomized Design with Factorial. There were two treatments, namely without pretreatment and with pretreatment and these were factorized with three levels of urea, namely 0, 1 and 2% with three replications. Finely dry ground banana peel samples were pretreated with NaOH 6% w/v. Simultaneous saccharification and fermentation was performed in 60 mL fermenter.  Sample (4% w/v) was placed in a fermenter and the following ingredients were added: 0.06 g KH2PO4, 0.06 g MgSO4 and citrate buffer with pH 5. Fermenter was enclosed and autoclaved at 121oC for 15 minutes and then cooled down at room temperature. Enzyme cellulose (6% w/v) and inoculum of Saccharomyces cerevisiae (10% v/v) were added into the fermenter. The mixture was incubated at 30oC for 9 days, and every day 3rd, 5th, 7th, and 9th was measured for reducing sugar content, pH and bioethanol content. Bioethanol content of the distillate was measured using picnometer.  Data was analyzed using ANOVA (Analysis of Variance) with Minitab 17 software. If there was significant (P<0.05) analysis continued with Tukey Pairwise comparison to find the difference among treatments. Results showed that treatment (without pretreatment and with pretreatment of NaOH 6% b/v) combined with levels of urea gave significant effect on reducing sugar and bioethanol content in all observations. Combination of without pretreatment and urea level at 1% and 2% resulted in the highest bioethanol content of 4,91% v/v.Bioethanol is one of the energy alternatives which environmentally friendly and sustainable. This research employed Completely Randomized Design with Factorial. There were two treatments, namely without pretreatment and with pretreatment and these were factorized with three levels of urea, namely 0, 1 and 2% with three replications. Finely dry ground banana peel samples were pretreated with NaOH 6% w/v. Simultaneous saccharification and fermentation was performed in 60 mL fermenter.  Sample (4% w/v) was placed in a fermenter and the following ingredients were added: 0.06 g KH2PO4, 0.06 g MgSO4 and citrate buffer with pH 5. Fermenter was enclosed and autoclaved at 121oC for 15 minutes and then cooled down at room temperature. Enzyme cellulose (6% w/v) and inoculum of Saccharomyces cerevisiae (10% v/v) were added into the fermenter. The mixture was incubated at 30oC for 9 days, and every day 3rd, 5th, 7th, and 9th was measured for reducing sugar content, pH and bioethanol content. Bioethanol content of the distillate was measured using picnometer.  Data was analyzed using ANOVA (Analysis of Variance) with Minitab 17 software. If there was significant (P<0.05) analysis continued with Tukey Pairwise comparison to find the difference among treatments. Results showed that treatment (without pretreatment and with pretreatment of NaOH 6% b/v) combined with levels of urea gave significant effect on reducing sugar and bioethanol content in all observations. Combination of without pretreatment and urea level at 1% and 2% resulted in the highest bioethanol content of 4,91% v/v.

Production and optimization of cellulase from agricultural waste and its application in bioethanol production by simultaneous saccharification and fermentation

2016 ◽  
Vol 27 (1) ◽  
pp. 22-35 ◽  
Author(s):  
Elsa Cherian ◽  
M. Dharmendira Kumar ◽  
G. Baskar
Keyword(s):  
Design Methodology ◽  
Simultaneous Saccharification And Fermentation ◽  
Agricultural Waste ◽  
Cellulase Production ◽  
Reducing Sugar ◽  
Bioethanol Production ◽  
Corn Cob ◽  
Content Type ◽  
Optimized Conditions ◽  
Simultaneous Saccharification

Purpose – The purpose of this paper is to optimize production of cellulase enzyme from agricultural waste by using Aspergillus fumigatus JCF. The study also aims at the production of bioethanol using cellulase and yeast. Design/methodology/approach – Cellulase production was carried out using modified Mandel’s medium. The optimization of the cellulase production was carried out using Plackett-Burman and Response surface methodology. Bioethanol production was carried out using simultaneous saccharification and fermentation. Findings – Maximum cellulase production at optimized conditions was found to be 2.08 IU/ml. Cellulase was used for the saccharification of three different feed stocks, i.e. sugar cane leaves, corn cob and water hyacinth. Highest amount of reducing sugar was released was 29.1 gm/l from sugarcane leaves. Sugarcane leaves produced maximum bioethanol concentration of 9.43 g/l out of the three substrates studied for bioethanol production. Originality/value – The present study reveals that by using the agricultural wastes, cellulase production can be economically increased thereby bioethanol production.

scholarly journals SOST Deficiency Aggravates Osteoarthritis in Mice by Promoting Sclerosis of Subchondral Bone

2019 ◽  
Vol 2019 ◽  
pp. 1-8
Author(s):  
Jingyu Li ◽  
Junjie Xue ◽  
Yan Jing ◽  
Manyi Wang ◽  
Rui Shu ◽  
...  
Keyword(s):  
Subchondral Bone ◽  
Cruciate Ligament ◽  
Cartilage Degradation ◽  
Cell Number ◽  
Blue Staining ◽  
Sham Operation ◽  
Knee Oa ◽  
Sost Gene ◽  
Bone Sclerosis ◽  
The Difference

As the initial part in the development of osteoarthritis (OA), subchondral bone sclerosis has been considered to be initiated by excess mechanical loading and proven to be correlated to other pathological changes. Sclerostin, which is an essential mechanical stress response protein, is encoded by the SOST gene. It is expressed in osteocytes and mature chondrocytes and has been proven to be closely correlated to OA. However, the relationship and mechanism between the SOST gene and the development of OA remain unclear. The aim of the present study was to investigate the role of the SOST gene in OA pathogenesis in the subchondral bone. A knee anterior cruciate ligament transection (ACLT) mouse osteoarthritis (OA) model on SOST-knockout (SOST KO) and wild-type (WT) mice was established. The pathogenic and phenotypic changes in the subchondral bone were investigated by histology, micro-CT, immunohistochemistry, TRAP staining, Masson staining, and Toluidine blue staining. It was found that sclerostin expression decreased in both the calcified cartilage and mineralized subchondral structures during the development of OA. Joint instability induced a severe cartilage degradation phenotype, with higher OARSI scores in SOST KO mice, when compared to WT mice. SOST KO mice with OA exhibited a higher BMD and BV/TV ratio, as well as a higher rate of bone remodeling and TRAP-positive cell number, when compared to the WT counterparts, but the difference was not significant between the sham-operation groups. It was concluded that loss of sclerostin aggravates knee OA in mice by promoting subchondral bone sclerosis and increasing catabolic activity of cartilage.

Pretreatment of Cocoa Waste for Bioethanol Production Using Ionic Liquid

2012 ◽  
Vol 59 (1) ◽  
Author(s):  
Ahmad Idi ◽  
Madihah Md. Salleh ◽  
Zaharah Ibrahim ◽  
Shaza Eva Mohamad
Keyword(s):  
Gas Chromatography ◽  
Ionic Liquid ◽  
Treatment Process ◽  
Fossil Fuel ◽  
Fermented Food ◽  
Reducing Sugar ◽  
Bioethanol Production ◽  
Ionic Liquid Pretreatment ◽  
Naoh Pretreatment ◽  
Biomass Loss

One of the major advantages of biofuel over fossil fuel is that it is environmentally friendly but unfortunately most of the chemicals used in the pretreatment of lignocelluloses biomass to produce biofuel can cause adverse effects to the environment. In this study, ionic liquid was used for the pretreatment of cocoa waste. Its effectiveness in the treatment process was compared to the alkalis and acids used in the conventional pretreatment media. The effectiveness of pretreatment using ionic liquid, H2SO4 and NaOH was based on the reduction of biomass, production of reducing sugar and also bioethanol. Ionic liquid pretreatment was found to show minimal biomass loss of only 31% after pretreatment compared to H2SO4 and NaOH which showed loss of 61% and 79% respectively. The untreated biomass has 10% amount of cellulose but upon pretreatment with ionic liquid, H2SO4 and NaOH, significant amount of cellulose was detected compared to NaOH which produced only 7% of cellulose. Two types of yeasts were also isolated from Malaysian local fermented food, the tapai ubi which were tested for the abilities to ferment the reducing sugar produced. Using the DNS method for determining reducing sugar, ionic liquid pretreatment was shown to produce 6.3×10–2g/L of reducing sugar while the untreated, H2SO4 and NaOH pretreatment produced 2.87×10–2g/L, 7.4×10–2g/L and 3.37×10–2g/L respectively at the end of 24 hours of incubation. Bioethanol produced during the fermentation was analysed using gas chromatography. Ionic liquid produced a total of 7.885g/L, H2SO4 produced 7.911g/L NaOH produced 6.824g/L and untreated cocoa waste produced 5.116g/L of ethanol at the end of 24 hours incubation.

scholarly journals Perbedaan kadar hemoglobin pada remaja gizi baik yang tinggal di pegunungan dengan yang tinggal di tepi pantai

e-CliniC ◽  
2016 ◽  
Vol 4 (1) ◽  
Author(s):  
Marianne C. Jacobus ◽  
Max F.J Mantik ◽  
Adrian . Umboh
Keyword(s):  
Analytical Study ◽  
Nutrition Status ◽  
P Value ◽  
Cross Sectional ◽  
Significant Difference ◽  
Good Nutrition ◽  
The Difference ◽  
Cross Sectional Design ◽  
And Gender ◽  
Main Component

Abstract: Haemoglobin is the main component of red blood cells that serves as a transporter of oxygen and carbon dioxide in the blood. The normal range of haemoglobin values can be used to determine the degree of anemia according to age and gender. Geographical condition such as altitude influences the haemoglobin value. This study aimed to obtain the difference of haemoglobin levels between teenagers with good nutrition status who live at the highland and those at the seaside. This was an observational analytical study with a cross sectional design. Samples were students with good nutrition status of SMPN 3 Tomohon (living at the highland) and those of SMP Kristen Nazaret Tuminting (living at the seaside). There were 60 students who met the inclusion criteria as follows: adolescent, good nutrition status, healthy, aged 13-15 years, lived at the highland or at the seaside ≥ 6 month, and willing to be performed blood examinations, and had been approved by their parents. The Mann-Whitney test for the difference between haemoglobin levels of the two groups showed a p value < 0,001. Conclusion: There was a very significant difference between haemoglobin levels of good nutrition teenagers who lived at the highland and at the seaside. Keywords: haemoglobin levels, good nutrition, teenagers, highland, seaside  Abstrak: Hemoglobin merupakan komponen utama sel darah merah dan berfungsi sebagai transporter oksigen dan karbon dioksida dalam darah. Batas normal nilai hemoglobin dapat digunakan untuk menetapkan derajat anemia, dengan distribusi usia dan jenis kelamin spesifik didasarkan pada sampel referensi sehat. Kondisi geografis, seperti ketinggian tempat dari permukaan laut menjadi faktor pertimbangan dalam distribusi nilai normal hemoglobin. Penelitian ini bertujuan untuk mendapatkan perbedaan kadar hemoglobin antara remaja gizi baik yang tinggal di pegunungan dengan yang tinggal di tepi pantai. Jenis penelitian ini analitik-observasional dengan desain potong lintang. Sampel penelitian yaitu remaja gizi baik yang tinggal di pegunungan yaitu siswa SMPN 3 Tomohon dan yang di tepi pantai yaitu siswa SMP Kristen Nazaret Tuminting. Subjek penelitian berjumlah 60 remaja yang memenuhi kriteria inklusi yaitu gizi baik, sehat, berumur 13-15 tahun, berdomisili di pegunungan atau tepi pantai ≥6 bulan, bersedia dilakukan pemeriksaan darah, dan telah disetujui oleh orang tua. Data yang diperoleh dianalisis menggunakan uji Mann-Whitney dengan bantuan program SPSS. Hasil uji Mann-Whitney mengenai perbedaan kadar hemoglobin antara kedua kelompok menunjukkan nila p <0,001. Simpulan: Terdapat perbedaan yang sangat bermakna kadar hemoglobin remaja gizi baik yang tinggal di pegunungan dengan di tepi pantai.Kata kunci: kadar hemoglobin, remaja gizi baik, pegunungan, tepi pantai

scholarly journals Evaluation of amniotic hydrogel’s impact on the development of murine pre-antral follicles in mouse model

2019 ◽  
Vol 2 (4) ◽  
pp. 32-39
Author(s):  
Van Ngoc Le Trinh ◽  
Hang Thi Kim Tran ◽  
Thu Thuy Anh Vo ◽  
Tuyet Thi Vi Le ◽  
Ha Le Bao Tran
Keyword(s):  
In Vitro Maturation ◽  
High Demand ◽  
Antral Follicles ◽  
Secondary Follicle ◽  
The Moment ◽  
Main Component ◽  
Supporting Frame ◽  
Mature Process

Global average infertility rate is about 6– 12%, and in Vietnam at around 7.7%. As a result, there is a high demand for treatment, especially for female infertility. In vitro maturation (IVM) was evaluated and proven to be the most popular and promising at the moment. In long-term cultivation, the follicle was observed to extend, therefore, the usage of a supporting frame is quite necessary to maintain follicle’s natural sphere structure as well as completing the mature process. Amniotic membrane is an avascular membrane, composed of collagen, fibronectin, nidogen, proteoglycan, containing a big number of growth – factors with antimicrobial, anti-inflammatory, low immunogenicity and viscoelasticity properties. Amniotic hydrogel owns structure formed with thin fibers to help preserve the main component as collagen, which can turn to gel form at 37 degree Celsius. With those properties, amniotic hydrogel showed high potential as a scaffold for the follicle. When amniotic hydrogel is used as a scaffold for cultivating of secondary follicle (100 – 130 µm), the size of oocyte and follicle increased after 12 days of culturing, along with the formation of antrum. The results demonstrated the possibility to use amniotic hydrogel as a scaffold for the development of the secondary follicle.

scholarly journals PRODUCTION OF BIOETHANOL FROM RICE AND MILLET HUSKS

2021 ◽  
pp. 1-12
Author(s):  
Gwandu AZ ◽  
Farouq AA ◽  
Baki AS ◽  
Peni DN
Keyword(s):  
Weight Loss ◽  
Thermogravimetric Analysis ◽  
Rice Husk ◽  
Sugar Content ◽  
Proximate Analysis ◽  
Reducing Sugar ◽  
High Yield ◽  
Bioethanol Production ◽  
Alternative Source ◽  
Fermentation Period

This study was based on the production of bioethanol as an alternative source of fuel using rice and millet husks. Proximate, elemental and thermogravimetric analysis (TGA) were conducted of the biomass. The proximate analysis revealed that millet husk had the highest moisture content of 26.67±0.58% when compared with rice husk 8.17±0.29%. The TGA of rice husk had a high endset temperature of 355.510C and a weight loss of -48.23%, millet husk had low endset temperature of 349.210C and a weight loss of -44.25%. When thirty grams (30g) of the substrates was used, rice husk revealed the highest reducing sugar content of 2.59±1.24mol/dm3 when compared to millet husk that had 1.63±0.68 mol/dm3. Rice husk indicated low yield after 7 days of fermentation period but showed a significantly high yield in the volume of ethanol produced 68.67±17.69g/l and millet husk had a yield of 79.80±0.93g/l. This indicated that both rice and millet husks were potent for bioethanol production with millet husk having the highest yield.

scholarly journals Joint regulation of cell size and cell number in the wing blade of Drosophila melanogaster

1997 ◽  
Vol 69 (1) ◽  
pp. 61-68 ◽  
Author(s):  
JENNIE McCABE ◽  
VERNON FRENCH ◽  
LINDA PARTRIDGE
Keyword(s):  
Drosophila Melanogaster ◽  
Significant Negative Correlation ◽  
Cell Number ◽  
Negative Association ◽  
Cell Area ◽  
Wing Area ◽  
Compensatory Control ◽  
Significant Difference ◽  
The Difference ◽  
Compensatory Regulation

We used Drosophila melanogaster to test for compensatory control of cell area and cell number in the regulation of total wing area. In two random bred wild-type base stocks collected from different geographic locations we found a negative association between the area and the number of cells in the wing blade. Three replicate lines were selected for increased or decreased wing area, with cell area maintained at the same level as in the three controls. After eight generations of selection, despite a large and highly significant difference in wing area between the large, control and small selection lines, cell area did not differ significantly between them. Rather, the difference in wing area between selection regimes was attributable to differences in cell number. Over the course of selection, the initially significant negative correlation between cell area and cell number in the wing increased, providing evidence for compensatory regulation of cell area and cell number. As a result of the increasingly negative association between the two traits, the variance in wing area declined as selection proceeded. It will be important to discover the mechanisms underlying the compensatory regulation of cell area and cell number.

The role of water-regulating mechanisms in the development of the haploid syndrome in Xenopus laevis

Development ◽  
1972 ◽  
Vol 28 (2) ◽  
pp. 449-462
Author(s):  
Louie Hamilton ◽  
P. H. Tuft
Keyword(s):  
Xenopus Laevis ◽  
Cell Number ◽  
Gastrula Stage ◽  
Tail Bud ◽  
Volume Changes ◽  
Membrane Area ◽  
Flow Through ◽  
The Difference ◽  
Haploid Embryos

The uptake of water by haploid and diploid sibling embryos of Xenopus laevis has been investigated by measuring the density changes which occur during the development of intact embryos from the blastula to the late tail-bud stage, and of explants from which most of the presumptive endoderm has been removed. The results show that up to the mid-gastrula stage there is no difference between the haploid and diploid embryos; but from then on, whereas the diploid volume increases steadily, the haploid gastrulae undergo a series of cyclical volume changes due to loss of fluid through the blastopore. It is concluded that this is the result of an excessive inflow of water through the haploid ectoderm, because it was found that the volume of haploid ectodermal explants increased much more rapidly than the volume of similar diploid explants. Excess flow through the haploid ectoderm also accounts for other characteristics of the haploid syndrome – microcephaly and lordosis. It is suggested that it is the doubling of the cell number in haploid embryos with the consequent 25% increase in aggregate cell membrane area which accounts for the difference between the uptake of water by the two types of embryos. It is also suggested that changes in the rate of water flow through the ectoderm and endoderm which are thought to account for the accumulation of water in the blastocoel and archenteron in the normal diploid embryo arise in a similar way.

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