Bioethanol Production from Cassava Peel Treated with Sulfonated Carbon Catalyzed Hydrolysis

A large amount of Cassava peel as biomass waste is generated by agricultural activities, and it led to a new pursuit to exploit the utilization of biomass waste. This research aimed to study the potential of Cassava peel as raw material for bioethanol production. This study was performed in 2 main processes, acid hydrolysis, and fermentation. The experiment was initiated by conducting acid hydrolysis (100°C and 60 min) on Cassava peel’s starch using sulfonated carbon catalyst palm oil empty fruit bunch (5%-w/v) to produce 13.53 g/L glucose. The glucose contained hydrolysates then continued to ferment at 30°C. The effect of fermentation time (h), pH, and shaking rate (rpm) of cassava peel’s starch fermentation using Saccharomyces cerevisiae was analyzed. The best result was found at pH 4.5 and 50 rpm for a 24 h reaction with 3.75 g/L of bioethanol concentration. This study revealed that Cassava peel is a promising feedstock for biofuel production.

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Analisis Kualitas Bioetanol Dari Kulit Pisang

Jurnal Daur Lingkungan ◽

10.33087/daurling.v4i2.72 ◽

2021 ◽

Vol 4 (2) ◽

pp. 35

Author(s):

G M Saragih ◽

Hadrah Hadrah ◽

Dilla Tri Maharani

Keyword(s):

Fermentation Process ◽

Starch Content ◽

Soil Surface ◽

Raw Material ◽

Banana Peel ◽

Total Production ◽

Bioethanol Production ◽

Fermentation Time ◽

Banana Production

Indonesia is ranked sixth in banana production with total production in 2015 of 7.299.275. the more people who like bananas, the higher the volume of banana peel waste produced. Banana peels are usually thrown away immediately and can contaminate the soil surface because banana peels contain acid so that it can have an impact on evironmental problems. The starch content of banan peels has the potential to be used as a raw material for bioethanol production with the help of the fermentation process. Therefore this research aims to determine the quality of bioethanol which is produced from several types of banana peels. The types of banana peels used are Ambon banana peel, Barangan banana peel and horn banana peel. The method used to manufacture bioethanol form the types of banana peel of ambon, barangan and horn is fermentation using yeast tape or saccharomyses cereviciae. The variables observed in this study were the variety of banana peel types, fermentation time for 6 days and 10 days, and the use of yeast as much as 5 grams. The fermentation results in the form of bioethanol were analyzed using gas chromatography, the best results from this study were obtained on the type of banana peel of Ambon for 10 days, that is 4.451% bioethanol.

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THE EFFECT OF THE TYPE OF ACID CATALYST AND TIME ON % YIELD OF BIOETHANOL FROM ELEPHANT GRASS (Pennistum Purpureum Schumach)

Jurnal Distilasi ◽

10.32502/jd.v4i2.2210 ◽

2020 ◽

Vol 4 (2) ◽

pp. 19

Author(s):

Netty - Herawati

Keyword(s):

Saccharomyces Cerevisiae ◽

Acid Catalyst ◽

Raw Material ◽

Cellulose Content ◽

Bioethanol Production ◽

Elephant Grass ◽

Fermentation Time ◽

Cattle Feed ◽

Good Nutrition ◽

High Cellulose

Elephant gass is cattle feed that contains good nutrition. One of its uses is converted into an energy source in the form bioethanol, Elephant grass has a high cellulose content reaching 40,85%, therefore elephant grass has the potential to be used as raw material in manufacture of bioethanol through the process of acid hydrolysis and fermentation. In research on percent yield of bioethanol from elephant grass chemically carried out at fixed conditions : grass weight 100 gr, temperature 100oC, water 1 liter, H2SO4 30 ml, hydrolysis timw 2 hours and conditions change : fermentation time 4,6,8 (day), saccharomyces cerevisiae starter 7%, 9%, 11%, 13%, HCl and H2SO4 catalys. From the research on chemical bioethanol production from elephant grass we got the best percent yield at 6 days of fermentation, 11% saccharomyces cerevisiae, HCl catalys which was 17,30%Keywords: bioethanol, fermentation, elephant grass,

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Making Bioethanol From Cocoa Fruit Skin Waste By Hydrolysis Process Using Trichoderma Viride Mold

Indonesian Journal of Chemical Science and Technology (IJCST) ◽

10.24114/ijcst.v2i1.12368 ◽

2020 ◽

Vol 2 (1) ◽

pp. 75

Author(s):

Tiska Oktavianis ◽

Sofiyanita Sofiyanita

Keyword(s):

Ethanol Fermentation ◽

Fermentation Process ◽

Trichoderma Viride ◽

Raw Material ◽

Bioethanol Production ◽

Fermentation Time ◽

Ethanol Content ◽

Hydrolysis Process ◽

Fruit Skin ◽

Fruit Waste

Cocoa fruit skin is one of the agricultural wastes can be used as raw material for bioethanol production. Because the cocoa fruit waste containing 39.45% crude fiber and 3.92% glucose. The purpose of this study was to determine the level of optimization of yeast and fermentation time to produce maximum ethanol content. In this study the hydrolysis process cocoa leather is done using fungi Trichoderma viride and fermentation process using yeast Saccharomyses cerevisiae. While for bioethanol concentration measurements performed using vinometer. The results showed that bioethanol fermentation time for 1, 3, 5 and 7 days using yeast levels 2, 4, 6 and 8 grams produce maximum ethanol fermentation at 3 days and 6 grams yeast levels. Produced a maximum ethanol content of 12%.

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Evaluation of Potential for Ethanol Production from Rice Straw Using Satellite Data

Journal of Agricultural Science ◽

10.5539/jas.v9n6p22 ◽

2017 ◽

Vol 9 (6) ◽

pp. 22

Author(s):

Chiharu Hongo ◽

Eisaku Tamura ◽

I. G. A. A. Ambarawati ◽

I. Made Anom Wijaya ◽

A. A. A. Mirah Adi

Keyword(s):

Rice Straw ◽

Satellite Data ◽

Rice Yield ◽

Storage System ◽

Field Investigation ◽

Raw Material ◽

Biofuel Production ◽

Bioethanol Production ◽

National Goal ◽

Paddy Area

Purpose of this study is to estimate rice yield on a plot basis with use of satellite data and field investigation data and to calculate potential quantity of rice straw to be utilized as a raw material for biofuel production in Bali, Indonesia. In addition, for continuous supply of rice straw by farmers to a biofuel producer, it is absolutely necessary to investigate farmer’s interest, behavior and potential issues to solve. For this investigation, an interview was made to the subak heads. The subak is a traditional social organization consisting of farmers for managing irrigation and agriculture in the farmers’ village.The created estimation equation of rice yield had NDVI from SPOT satellite data as a predictor and was significant at 1% level. Based on the rice yield estimated through the equation and the paddy area, quantity of rice straw to be available and quantity of bioethanol to be produced were estimated. In case of Kediri, the rice straw quantity was 42,274 t/year and the ethanol quantity was 12,682 kl.On the other hand, the handling of rice straw after harvesting was leaving/putting back to soil or burning, which accounted for 76% of the rice straw. Concerning a price of rice straw, about 60% of farmers expressed their willingness to sell at 100 rupiah (1 cent US$) per kg, and about 88% including people saying high probability seemed to think this price would be as an adequate level of sales price. Through the interview work, it becomes clear that, when some issues such as price of rice straw are settled, farmers are positive to selling of rice straw for production of biofuel.For realizing bioethanol production from rice straw, there still remain some items to be studied further such as production process, transportation and storage system and costs. The result of our study suggests that a proposal for improvement of stable production can be made through rice yield estimation and monitoring using satellite data and that rice straw can be supplied as a promising resource of raw material for bioethanol production. This is considered to contribute the promotion of activity to reach the national goal of bioethanol production in future.

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Pretreatment Lignoselulosa dari Jerami Padi dengan Deep Eutectic Solvent untuk Meningkatkan Produksi Bioetanol Generasi Dua (Lignocellulose Pretreatment of Rice Straw using Deep Eutectic Solvent to Increase Second-Generation Bioethanol Production)

Jurnal Riset Industri Hasil Hutan ◽

10.24111/jrihh.v10i1.4079 ◽

2018 ◽

Vol 10 (1) ◽

pp. 43-54 ◽

Cited By ~ 2

Author(s):

Nurwahdah Nurwahdah ◽

Al-Arofatus Naini ◽

Asma Nadia ◽

Ratri Yuli Lestari ◽

Sunardi Sunardi, Ph.D.

Keyword(s):

Fossil Fuels ◽

Second Generation ◽

Deep Eutectic Solvent ◽

Production Costs ◽

Fuel Oil ◽

Raw Material ◽

Chemical Components ◽

Bioethanol Production ◽

Biomass Waste ◽

Second Generation Bioethanol

Current issues of energy sector in Indonesia can be summarized as depletion of fossil energy reserves which is dominated by fuel oil and coal. Oil production continues to decline and the increase in oil fuels demand lead to increase imports of crude oil and oil fuels. To use lignocellulosic biomass waste has become a major alternative to replace fossil fuels and chemical feedstocks production. In 2015, total rice production in South Kalimantan reached 2,140,276 ton and rice straws were abundant waste which could be utilized as raw material for bioethanol production. Pretreatment process of lignocellulose is a crucial step to remove lignin because of the complex chemical cross-linking between chemical components. Delignification of lignin can increase the accessibility and digestibility of enzymatic, and help to promote enzymatic hydrolysis. Nowadays, pretreatment process with green chemistry method is continuesly developed by researcher to reduce the production costs and thus avoid adverse effects on human and the environment. This article disscussed about green methods for pretreatment of lignocellulosic material using deep eutectic solvent (DES) to increase second-generation bioethanol production in South Kalimantan.

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Bioethanol (Biofuel) Production from Low Grade Dates

Iraqi Journal of Chemical and Petroleum Engineering ◽

10.31699/ijcpe.2019.4.7 ◽

2019 ◽

Vol 20 (4) ◽

pp. 41-47

Author(s):

Raghad Ali Abbas ◽

Hussain M. Flayeh

Keyword(s):

Ph Value ◽

Carbon Sources ◽

Aerobic Condition ◽

Inoculum Size ◽

Biofuel Production ◽

Sugar Concentration ◽

Low Grade ◽

Bioethanol Production ◽

Fermentation Time ◽

Sugar Fermentation

Bioethanol production from sugar fermentation is one of the most sustainable alternatives to substitute fossil fuel. production of bioethanol from low grade dates which are rich of sugars. An available sugar from a second grade dates (reduction sugar) was 90g/l in this study. Sugar can be served as essential carbon sources for yeast growth in aerobic condition and can also be converted to bioethanol in anaerobic condition. The effect of various parameters on bioethanol production, fermentation time, pH-values, inoculum size and initial sugar concentration were varied in order to determine the optimal of bioethanol production. The highest bioethanol yield was 33g/l which was obtained with sugar concentration 90 g/l, inoculum size 1%, 52h time and pH-value 5.

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Brewers’ spent grain as substrate for dextran biosynthesis by Leuconostoc pseudomesenteroides DSM20193 and Weissella confusa A16

Microbial Cell Factories ◽

10.1186/s12934-021-01515-4 ◽

2021 ◽

Vol 20 (1) ◽

Author(s):

Prabin Koirala ◽

Ndegwa Henry Maina ◽

Hanna Nihtilä ◽

Kati Katina ◽

Rossana Coda

Keyword(s):

Lactic Acid ◽

Lactic Acid Bacteria ◽

Raw Materials ◽

Degree Of Polymerization ◽

Raw Material ◽

Fermentation Time ◽

Viscosity Increase ◽

Spent Grain ◽

Leuconostoc Pseudomesenteroides ◽

Weissella Confusa

Abstract Background Lactic acid bacteria can synthesize dextran and oligosaccharides with different functionality, depending on the strain and fermentation conditions. As natural structure-forming agent, dextran has proven useful as food additive, improving the properties of several raw materials with poor technological quality, such as cereal by-products, fiber-and protein-rich matrices, enabling their use in food applications. In this study, we assessed dextran biosynthesis in situ during fermentation of brewers´ spent grain (BSG), the main by-product of beer brewing industry, with Leuconostoc pseudomesenteroides DSM20193 and Weissella confusa A16. The starters performance and the primary metabolites formed during 24 h of fermentation with and without 4% sucrose (w/w) were followed. Results The starters showed similar growth and acidification kinetics, but different sugar utilization, especially in presence of sucrose. Viscosity increase in fermented BSG containing sucrose occurred first after 10 h, and it kept increasing until 24 h concomitantly with dextran formation. Dextran content after 24 h was approximately 1% on the total weight of the BSG. Oligosaccharides with different degree of polymerization were formed together with dextran from 10 to 24 h. Three dextransucrase genes were identified in L. pseudomesenteroides DSM20193, one of which was significantly upregulated and remained active throughout the fermentation time. One dextransucrase gene was identified in W. confusa A16 also showing a typical induction profile, with highest upregulation at 10 h. Conclusions Selected lactic acid bacteria starters produced significant amount of dextran in brewers’ spent grain while forming oligosaccharides with different degree of polymerization. Putative dextransucrase genes identified in the starters showed a typical induction profile. Formation of dextran and oligosaccharides in BSG during lactic acid bacteria fermentation can be tailored to achieve specific technological properties of this raw material, contributing to its reintegration into the food chain.

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Improved Glucose Recovery from Sicyos angulatus by NaOH Pretreatment and Application to Bioethanol Production

Processes ◽

10.3390/pr9020245 ◽

2021 ◽

Vol 9 (2) ◽

pp. 245

Author(s):

Hyung-Eun An ◽

Kang Hyun Lee ◽

Ye Won Jang ◽

Chang-Bae Kim ◽

Hah Young Yoo

Keyword(s):

Saccharomyces Cerevisiae ◽

Alternative Energy ◽

Invasive Plant ◽

Food Resource ◽

Raw Material ◽

Control Group ◽

Bioethanol Production ◽

Naoh Pretreatment ◽

Glucose Recovery ◽

Harmful Species

As greenhouse gases and environmental pollution become serious, the demand for alternative energy such as bioethanol has rapidly increased, and a large supply of biomass is required for bioenergy production. Lignocellulosic biomass is the most abundant on the planet and a large part of it, the second-generation biomass, has the advantage of not being a food resource. In this study, Sicyos angulatus, known as an invasive plant (harmful) species, was used as a raw material for bioethanol production. In order to improve enzymatic hydrolysis, S. angulatus was pretreated with different NaOH concentration at 121 °C for 10 min. The optimal NaOH concentration for the pretreatment was determined to be 2% (w/w), and the glucan content (GC) and enzymatic digestibility (ED) were 46.7% and 55.3%, respectively. Through NaOH pretreatment, the GC and ED of S. angulatus were improved by 2.4-fold and 2.5-fold, respectively, compared to the control (untreated S. angulatus). The hydrolysates from S. angulatus were applied to a medium for bioethanol fermentation of Saccharomyces cerevisiae K35. Finally, the maximum ethanol production was found to be 41.3 g based on 1000 g S. angulatus, which was 2.4-fold improved than the control group.

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Tagasaste, leucaena and paulownia: three industrial crops for energy and hemicelluloses production

Biotechnology for Biofuels ◽

10.1186/s13068-021-01930-0 ◽

2021 ◽

Vol 14 (1) ◽

Author(s):

Alberto Palma ◽

Javier Mauricio Loaiza ◽

Manuel J. Díaz ◽

Juan Carlos García ◽

Inmaculada Giráldez ◽

...

Keyword(s):

Activation Energy ◽

Acid Hydrolysis ◽

Energy Production ◽

Combustion Process ◽

Operating Conditions ◽

Raw Material ◽

Energy Of Combustion ◽

Increasing Temperature ◽

Hydrolysis Of ◽

Chamaecytisus Proliferus

Abstract Background Burning fast-growing trees for energy production can be an effective alternative to coal combustion. Thus, lignocellulosic material, which can be used to obtain chemicals with a high added value, is highly abundant, easily renewed and usually inexpensive. In this work, hemicellulose extraction by acid hydrolysis of plant biomass from three different crops (Chamaecytisus proliferus, Leucaena diversifolia and Paulownia trihybrid) was modelled and the resulting solid residues were used for energy production. Results The influence of the nature of the lignocellulosic raw material and the operating conditions used to extract the hemicellulose fraction on the heat capacity and activation energy of the subsequent combustion process was examined. The heat power and the activation energy of the combustion process were found to depend markedly on the hemicellulose content of the raw material. Thus, a low content in hemicelluloses resulted in a lower increased energy yield after acid hydrolysis stage. The process was also influenced by the operating conditions of the acid hydrolysis treatment, which increased the gross calorific value (GCV) of the solid residue by 0.6–9.7% relative to the starting material. In addition, the activation energy of combustion of the acid hydrolysis residues from Chamaecytisus proliferus (Tagasaste) and Paulownia trihybrid (Paulownia) was considerably lower than that for the starting materials, the difference increasing with increasing degree of conversion as well as with increasing temperature and acid concentration in the acid hydrolysis. The activation energy of combustion of the solid residues from acid hydrolysis of tagasaste and paulownia decreased markedly with increasing degree of conversion, and also with increasing temperature and acid concentration in the acid hydrolysis treatment. No similar trend was observed in Leucaena diversifolia (Leucaena) owing to its low content in hemicelluloses. Conclusions Acid hydrolysis of tagasaste, leucaena and paulownia provided a valorizable liquor containing a large amount of hemicelluloses and a solid residue with an increased heat power amenable to efficient valorization by combustion. There are many potential applications of the hemicelluloses-rich and lignin-rich fraction, for example as multi-components of bio-based feedstocks for 3D printing, for energy and other value-added chemicals.

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Diverse Banana Pseudostems and Rachis Are Distinctive for Edible Carbohydrates and Lignocellulose Saccharification towards High Bioethanol Production under Chemical and Liquid Hot Water Pretreatments

Molecules ◽

10.3390/molecules26133870 ◽

2021 ◽

Vol 26 (13) ◽

pp. 3870

Author(s):

Jingyang Li ◽

Fei Liu ◽

Hua Yu ◽

Yuqi Li ◽

Shiguang Zhou ◽

...

Keyword(s):

Soluble Sugars ◽

Enzymatic Saccharification ◽

Hot Water ◽

Biofuel Production ◽

Bioenergy Crops ◽

Bioethanol Production ◽

Factors Affecting ◽

Liquid Hot Water ◽

Biomass Processing ◽

Banana Crops

Banana is a major fruit crop throughout the world with abundant lignocellulose in the pseudostem and rachis residues for biofuel production. In this study, we collected a total of 11 pseudostems and rachis samples that were originally derived from different genetic types and ecological locations of banana crops and then examined largely varied edible carbohydrates (soluble sugars, starch) and lignocellulose compositions. By performing chemical (H2SO4, NaOH) and liquid hot water (LHW) pretreatments, we also found a remarkable variation in biomass enzymatic saccharification and bioethanol production among all banana samples examined. Consequently, this study identified a desirable banana (Refen1, subgroup Pisang Awak) crop containing large amounts of edible carbohydrates and completely digestible lignocellulose, which could be combined to achieve the highest bioethanol yields of 31–38% (% dry matter), compared with previously reported ones in other bioenergy crops. Chemical analysis further indicated that the cellulose CrI and lignin G-monomer should be two major recalcitrant factors affecting biomass enzymatic saccharification in banana pseudostems and rachis. Therefore, this study not only examined rich edible carbohydrates for food in the banana pseudostems but also detected digestible lignocellulose for bioethanol production in rachis tissue, providing a strategy applicable for genetic breeding and biomass processing in banana crops.

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