Preliminary analysis of Plasmodium vivax genotypes isolated in southeastern Turkey

2015 ◽  
Vol 60 (2) ◽  
Author(s):  
Aysu Değirmenci Döşkaya ◽  
Mert Döşkaya ◽  
Ayşe Caner ◽  
Kadri Gül ◽  
Şebnem Nergiz ◽  
...  
Keyword(s):  
Genetic Polymorphism ◽  
Plasmodium Vivax ◽  
Preliminary Analysis ◽  
Blood Samples ◽  
Common Cause ◽  
Three Samples ◽  
Two Samples ◽  
Pcr Rflp ◽  
New Treatment ◽  
First Time

AbstractPlasmodium vivax is the most common cause of malaria worldwide as well as southeastern Turkey. After the implementation of a successful national elimination program that the local malaria cases were not reported in 2011, malaria returned to county of Savur located in southeastern Turkey in summer of 2012. The present study aimed to determine the prevalent P. vivax genotypes isolated from southeastern Turkey. Genetic polymorphism in P. vivax CSP gene was analyzed by PCR-RFLP to assess the ratio of VK210 and VK247 types. Blood samples were obtained from 15 patients who lived in southeastern between 2005-2006. According to the results, VK210 type was detected in 10 samples (66.6%), VK247 type was observed in three samples (20%). Remaining two samples showed mixed infection (13.3%). The results of the present study first time showed the ratio of P. vivax genotypes in southeastern Turkey before the elimination in 2011. The results of the present study will be enable researchers to compare the new isolates with the previously detected ones and design new treatment and/elimination strategies.

scholarly journals Effect of leptin genetic polymorphism on lameness prevalence in Tunisian Holstein cows

2018 ◽  
Vol 61 (3) ◽  
pp. 305-310 ◽  
Author(s):  
Mohamed Amine Ferchichi ◽  
Bayrem Jemmali ◽  
Sihem Amiri ◽  
Abderrahmane Ben Gara ◽  
Boulbaba Rekik
Keyword(s):  
Logistic Regression ◽  
Genetic Polymorphism ◽  
Restriction Enzyme ◽  
Logistic Regression Model ◽  
Genetic Factors ◽  
Holstein Cows ◽  
Blood Samples ◽  
The Third ◽  
Pcr Rflp ◽  
Calving Season

Abstract. Lameness is the third most important infection affecting dairy cattle in modern stabling barns. Environmental (food, stables and injuries) and genetic factors are responsible for this pathology. This study aims to investigate the screening of leptin genetic polymorphism and its effect on lameness prevalence. DNA was extracted from 412 blood samples from Holstein cows (lame and healthy). Genotyping was performed by the PCR-RFLP using the Sau3AI restriction enzyme. Prevalence of parity, calving season, age at calving, test-day milk yields, calving year and leptin/Sau3AI genotype was evaluated by the logistics procedure. Results showed that allelic frequencies are 0.68 and 0.32 respectively for the A and B allele. Frequencies for the AA, AB and BB genotypes are 0.52, 032 and 0.16 respectively. All factors included in the logistic regression model significantly affect the prevalence of lameness in Holstein cows (P<0.01). The probability of lameness incidence varied between 37 % and 99 % respectively for the second and the third parity. The lameness incidence recorded in multiparous is higher than primiparous cows. This recorded incidence is greater in winter and autumn than in the summer and spring. The genetic polymorphism affects the prevalence of lameness in Holstein cows. The incidence of lameness in AB genotype is lower than that in BB and AA genotype. These results can be a way to improve length of productive life for cattle breeds in Tunisia.

scholarly journals Chemical Fingerprinting of Seeds of Some Salvia Species in Turkey by Using GC-MS and FTIR

Foods ◽  
2019 ◽  
Vol 8 (4) ◽  
pp. 118 ◽  
Author(s):  
Eray Tulukcu ◽  
Nur Cebi ◽  
Osman Sagdic
Keyword(s):  
Volatile Compounds ◽  
Principal Component ◽  
Hierarchical Cluster ◽  
Three Samples ◽  
Two Samples ◽  
Chemical Fingerprinting ◽  
Reflectance Ftir ◽  
Gas Chromatography Mass Spectroscopy ◽  
First Time

Six species of Salvia seeds cultivated and grown in Cumra/Konya (Turkey) were evaluated using headspace gas chromatography mass spectroscopy (GC-MS) and Fourier transform infrared spectroscopy-attenuated total reflectance (FTIR-ATR) combined chemometrics of hierarchical cluster analysis (HCA) and principal component analysis (PCA). The major volatile compounds in the Salvia species are determined as n-hexanal (present in seven samples), sabinene (present in three samples), α-pinene (present in 13 samples), α-thujone (present in four samples), borneol (present in 11 samples), linalyl acetate (present in 10 samples), β-pinene (present in 13 samples), camphene (present in 13 samples), α-thujene (present in four samples), 2,4(10)-thujadien (present in two samples), β-myrcene (present in seven samples), limonen (present in 12 samples), 1,8-cineole (eucalyptol) (present in 13 samples) and camphor (present in nine samples). The most abundant (%) volatile compounds among all were detected as α-pinene, camphene, β-pinene and eucalyptol. For the first time, chemometrics of HCA and PCA is applied to FTIR and GC-MS data. The classification of all samples is performed on the basis of their chemical similarities and differences.

Genetic polymorphism in patients with colorectal and with head and neck cancer

2009 ◽  
Vol 150 (33) ◽  
pp. 1545-1549 ◽  
Author(s):  
András Csejtei ◽  
Antal Tibold ◽  
István Ember ◽  
István Kiss
Keyword(s):  
Head And Neck Cancer ◽  
Genetic Polymorphism ◽  
Head And Neck ◽  
Neck Cancer ◽  
Pcr Rflp ◽  
Xrcc1 Arg194trp

A colorectalis rákok a második leggyakoribb halálokként szerepelnek a rosszindulatú betegségek között. A fej-nyak táji daganatok halálozása Magyarországon 265%-kal emelkedett az utóbbi 30 évben. Nem lehet eléggé hangsúlyozni e két daganatcsoport közegészségügyi jelentőségét. A colorectalis daganatok esetén a GSTM1, GSTT1 enzimek, valamint a p53 72-es kodonjának, fej-nyak táji tumorok esetén az XRCC1 Arg194Trp és Arg399Gln polimorfizmusainak hatását elemeztük. Intraoperatíve eltávolított daganatos és megfelelően illesztett daganatmentes mintákat válogattunk. A formalinban fixált mintákat deparaffinizáltuk és proteináz-K-emésztésnek vetettük alá. A genotipizálást PCR, illetve a fej-nyak táji tumorok esetén PCR-RFLP módszerrel végeztük. A vizsgált allélek gyakoriságában nem volt különbség a daganatos és a kontrollcsoport között. Túlélés tekintetében szignifikáns különbséget találtunk a GSTM1 és a p53 allélek között Dukes B stádiumú colorectalis daganatok esetén és az XRCC1 194 allélek között III-as stádiumú fej-nyak táji tumorokban. A fenti típusú genetikai különbségek szisztematikus vizsgálata a jövőben hozzájárulhat az egyéni rizikóbecslés és az individualizált kezelések megalapozásához.

Application of Novel Low Current OBIRCH Amplifier and Nanoprobing to Identify Subtle Leakages in Advanced Node Technologies

2018 ◽  
Author(s):  
Satish Kodali ◽  
Liangshan Chen ◽  
Yuting Wei ◽  
Tanya Schaeffer ◽  
Chong Khiam Oh
Keyword(s):  
Signal To Noise Ratio ◽  
Semiconductor Industry ◽  
Fault Isolation ◽  
Ring Oscillator ◽  
High Signal ◽  
The Novel ◽  
Resistance Change ◽  
Three Samples ◽  
Relative Threshold ◽  
First Time

Abstract Optical beam induced resistance change (OBIRCH) is a very well-adapted technique for static fault isolation in the semiconductor industry. Novel low current OBIRCH amplifier is used to facilitate safe test condition requirements for advanced nodes. This paper shows the differences between the earlier and novel generation OBIRCH amplifiers. Ring oscillator high standby leakage samples are analyzed using the novel generation amplifier. High signal to noise ratio at applied low bias and current levels on device under test are shown on various samples. Further, a metric to demonstrate the SNR to device performance is also discussed. OBIRCH analysis is performed on all the three samples for nanoprobing of, and physical characterization on, the leakage. The resulting spots were calibrated and classified. It is noted that the calibration metric can be successfully used for the first time to estimate the relative threshold voltage of individual transistors in advanced process nodes.

scholarly journals Molecular Detection and Phylogeny of Anaplasmaphagocytophilum and Related Variants in Small Ruminants from Turkey

Animals ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 814
Author(s):  
Münir Aktaş ◽  
Sezayi Özübek ◽  
Mehmet Can Uluçeşme
Keyword(s):  
16S Rrna ◽  
Small Ruminants ◽  
Rrna Gene ◽  
Infection Rates ◽  
Sheep And Goats ◽  
Significant Difference ◽  
Pcr Rflp ◽  
First Time ◽  
Japan And China ◽  
Apparently Healthy

Anaplasma phagocytophilum causes tick-borne fever in small ruminants. Recently, novel Anaplasma variants related to A. phagocytophilum have been reported in ruminants from Tunisia, Italy, South Korea, Japan, and China. Based on 16S rRNA and groEL genes and sequencing, we screened the frequency of A. phagocytophilum and related variants in 433 apparently healthy small ruminants in Turkey. Anaplasma spp. overall infection rates were 27.9% (121/433 analyzed samples). The frequency of A. phagocytophilum and A. phagocytophilum-like 1 infections was 1.4% and 26.5%, respectively. No A. phagocytophilum-like 2 was detected in the tested animals. The prevalence of Anaplasma spp. was comparable in species, and no significant difference was detected between sheep and goats, whereas the prevalence significantly increased with tick infestation. Sequencing confirmed PCR-RFLP data and showed the presence of A. phagocytophilum and A. phagocytophilum-like-1 variant in the sampled animals. Phylogeny-based on 16S rRNA gene revealed the A. phagocytophilum-like 1 in a separate clade together with the previous isolates detected in small ruminants and ticks. In this work, A. phagocytophilum-like 1 has been detected for the first time in sheep and goats from Turkey. This finding revealed that the variant should be considered in the diagnosis of caprine and ovine anaplasmosis.

scholarly journals Biosystematic Study on Some Egyptian Species of Astragalus L. (Fabaceae)

Agriculture ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 125
Author(s):  
Monier M. Abd El-Ghani ◽  
Ashraf S. A. El-Sayed ◽  
Ahmed Moubarak ◽  
Rabab Rashad ◽  
Hala Nosier ◽  
...  
Keyword(s):  
Genetic Relationships ◽  
Dna Barcode ◽  
Plant Morphology ◽  
Its Region ◽  
Its2 Region ◽  
Whole Plant ◽  
Three Samples ◽  
Two Samples ◽  
Region 10 ◽  
Unique Banding Pattern

Astragalus L. is one of the largest angiosperm complex genera that belongs to the family Fabaceae, subfamily Papilionoideae or Faboideae under the subtribe Astragalinae of the tribe Galegeae. The current study includes the whole plant morphology, DNA barcode (ITS2), and molecular marker (SCoT). Ten taxa representing four species of Astragalus were collected from different localities in Egypt during the period from February 2018 to May 2019. Morphologically, identification and classification of collected Astragalus plants occurred by utilizing the light microscope, regarding the taxonomic revisions of the reference collected Astragalus specimens in other Egyptian Herbaria. For molecular validation, ten SCoT primers were used in this study, producing a unique banding pattern to differentiate between ten samples of Astragalus taxa which generated 212 DNA fragments with an average of 12.2 bands per 10 Astragalus samples, with 8 to 37 fragments per primer. The 212 fragments amplified were distributed as 2 monomorphic bands, 27 polymorphic without unique bands, 183 unique bands (210 Polymorphic with unique bands), and ITS2 gene sequence was showed as the optimal barcode for identifying Astragalus L. using BLAST searched on NCBI database, and afterward, analyzing the chromatogram for ITS region, 10 samples have been identified as two samples representing A. hauarensis, four samples representing A. sieberi, three samples representing A. spinosus and one sample representing A. vogelii. Based on the ITS barcode, A. hauarensis RMG1, A. hauarensis RMG2, A. sieberi RMG1, A. sieberi RMG2, A. sieberi RMG3, A. sieberi RMG4, A. spinosus RMG1, A. spinosus RMG2, A. spinosus RMG3, A. vogelii RMG were deposited into GenBank with accession # MT367587.1, MT367591.1, MT367593.1, MT367585.1, MT367586.1, MT367588.1, MT160347.1, MT367590.1, MT367589.1, MT367592.1, respectively. These results indicated the efficiency of SCoT markers and ITS2 region in identifying and determining genetic relationships between Astragalus species.

scholarly journals A Comparison of the Composition of Selected Commercial Sandalwood Oils with the International Standard

Molecules ◽  
2021 ◽  
Vol 26 (8) ◽  
pp. 2249
Author(s):  
Malgorzata Kucharska ◽  
Barbara Frydrych ◽  
Wiktor Wesolowski ◽  
Jadwiga A. Szymanska ◽  
Anna Kilanowicz
Keyword(s):  
Mass Spectrometry Analysis ◽  
Santalum Album ◽  
Benzyl Benzoate ◽  
Spectrometry Analysis ◽  
Correct Information ◽  
Three Samples ◽  
Two Samples ◽  
High Street ◽  
Chromatographic Profile ◽  
Sandalwood Oil

Sandalwood oils are highly desired but expensive, and hence many counterfeit oils are sold in high street shops. The study aimed to determine the content of oils sold under the name sandalwood oil and then compare their chromatographic profile and α- and β santalol content with the requirements of ISO 3518:2002. Gas chromatography with mass spectrometry analysis found that none of the six tested “sandalwood” oils met the ISO standard, especially in terms of α-santalol content. Only one sample was found to contain both α- and β-santalol, characteristic of Santalum album. In three samples, valerianol, elemol, eudesmol isomers, and caryophyllene dominated, indicating the presence of Amyris balsamifera oil. Another two oil samples were found to be synthetic mixtures: benzyl benzoate predominating in one, and synthetic alcohols, such as javanol, polysantol and ebanol, in the other. The product label only gave correct information in three cases: one sample containing Santalum album oil and two samples containing Amyris balsamifera oil. The synthetic samples described as 100% natural essential oil from sandalwood are particularly dangerous and misleading to the consumer. Moreover, the toxicological properties of javanol, polysantol and ebanol, for example, are unknown.

scholarly journals Serially measured cytokines and cytokine receptors in relation to clinical outcome in patients with stable heart failure

2020 ◽  
Vol 41 (Supplement_2) ◽  
Author(s):  
E Bouwens ◽  
A Schuurman ◽  
K.M Akkerhuis ◽  
S.J Baart ◽  
K Caliskan ◽  
...  
Keyword(s):  
Heart Failure ◽  
Clinical Outcome ◽  
Primary Endpoint ◽  
Nyha Class ◽  
Cytokine Receptors ◽  
Funding Source ◽  
Compensatory Mechanism ◽  
Blood Samples ◽  
Two Samples

Abstract Background Activation of the inflammatory response in heart failure (HF) may initially serve as a compensatory mechanism. However, on the longer term, this physiological phenomenon can become disadvantageous. Temporal patterns of inflammatory proteins other than CRP have not yet been investigated in patients with stable HF. Purpose We aimed to evaluate the association of 17 serially measured cytokines and cytokine receptors with clinical outcome in patients with stable heart failure. Methods In 263 patients, 1984 serial, tri-monthly blood samples were collected during a median follow-up of 2.2 (IQR: 1.4–2.5) years. The primary endpoint (PE) composed of cardiovascular mortality, HF-hospitalization, heart transplantation, and LVAD. We selected baseline blood samples in all patients, as well as the two samples closest to the primary endpoint, and the last sample available in event-free patients. Thus, in 567 samples we measured 17 cytokines and cytokine receptors using the Olink Proteomics Cardiovascular III multiplex assay. Associations between biomarkers and PE were investigated by joint modelling. Results Median age was 68 (IQR: 59–76) years, with 72% men, 74% NYHA class I-II and a median ejection fraction of 30% (23–38%). 70 patients reached a PE. After adjustment for clinical characteristics (age, sex, diabetes, atrial fibrillation, NYHA class at baseline, diuretics and systolic blood pressure), 7 biomarkers were associated with the PE (Figure). Interleukin-1 receptor type 1 (IL1RT1) showed the strongest association: HR 2.65 [95% CI: 1.78–4.21]) per standard deviation change in level (NPX) at any point in time during follow-up, followed by Tumor necrosis factor receptor 1 (TNF-R1): 2.25 [1.66–3.08], and C-X-C motif chemokine 16 (CXCL16): 2.18 [1.59–3.04]. After adjustment for baseline N-terminal pro–B-type natriuretic peptide, high-sensitive troponin T and C-reactive protein however, only IL1RT1 and TNF-R1 remained significantly associated with the PE. Conclusion Repeatedly measured levels of several cytokines and cytokine receptors are independently associated with clinical outcome in stable HF patients. These results suggest that repeated measurements of these biomarkers, in addition to established cardiac biomarkers, may contribute to personalized risk assessment and herewith better identify high-risk patients. Figure 1. Associations between levels of cytokines and cytokine receptors and the primary endpoint. Funding Acknowledgement Type of funding source: Other. Main funding source(s): This work was supported by the Jaap Schouten Foundation and the Noordwest Academie.

Genetic polymorphism of vir genes of Plasmodium vivax in Myanmar

2021 ◽  
Vol 80 ◽  
pp. 102233
Author(s):  
Byoung-Kuk Na ◽  
Tong-Soo Kim ◽  
Khin Lin ◽  
Moon-Chang Baek ◽  
Dong-Il Chung ◽  
...  
Keyword(s):  
Genetic Polymorphism ◽  
Plasmodium Vivax ◽  
Vir Genes

scholarly journals Antimicrobial Activity of L-Lysine and Poly-L-Lysine with Pulsed Electric Fields

2021 ◽  
Vol 11 (6) ◽  
pp. 2708
Author(s):  
Jurgita Švedienė ◽  
Vitalij Novickij ◽  
Rokas Žalnėravičius ◽  
Vita Raudonienė ◽  
Svetlana Markovskaja ◽  
...  
Keyword(s):  
Electric Fields ◽  
Pulsed Electric Fields ◽  
Antimicrobial Treatment ◽  
Treatment Development ◽  
E Coli ◽  
Yeast Fungi ◽  
New Treatment ◽  
First Time ◽  
Resistant Microorganism ◽  
Susceptibility Patterns

For the first time, the possibility to use L-lysine (Lys) and poly-L-lysine (PLL) as additives with pulsed electric fields (PEF) for antimicrobial treatment is reported. The antimicrobial efficacy of Lys and PLL for Escherichia coli, Staphylococcus aureus, Trichophyton rubrum and Candida albicans was determined. Inactivation of microorganisms was also studied by combining Lys and PLL with PEF of 15 and 30 kV/cm. For PEF treatment, pulses of 0.5, 1, 10 or 100 μs were applied in a sequence of 10 to 5000 at 1 kHz frequency. The obtained results showed that 100 μs pulses were the most effective in combination with Lys and PLL for all microorganisms. Equivalent energy PEF bursts with a shorter duration of the pulse were less effective independently on PEF amplitude. Additionally, various treatment susceptibility patterns of microorganisms were determined and reported. In this study, the Gram-negative E. coli was the most treatment-resistant microorganism. Nevertheless, inactivation rates exceeding 2 log viability reduction were achieved for all analyzed yeast, fungi, and bacteria. This methodology could be used for drug-resistant microorganism’s new treatment development.

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