A trial of doramectin injection and ivermectin spot-on for treatment of rabbits artificially infested with the ear mite “Psoroptes cuniculi”

2017 ◽  
Vol 20 (3) ◽  
pp. 521-525 ◽  
Author(s):  
N.M. Elhawary ◽  
Sh. S.G.H. Sorour ◽  
M.A. El-Abasy ◽  
E.K. Bazh ◽  
K. Sultan
Keyword(s):  
Single Dose ◽  
New Zealand ◽  
Great Improvement ◽  
Skin Disease ◽  
Positive Control ◽  
Control Group ◽  
Therapeutic Trial ◽  
Psoroptes Cuniculi ◽  
New Zealand White Rabbits ◽  
The World

Abstract The ear mite “Psoroptes cuniculi” is the main cause of ear mange, a highly contagious parasitic skin disease in rabbits all over the world. In the current work, a preliminary therapeutic trial to study the effect of the broad use acaricides doramectin and ivermectin on P. cuniculi was performed on artificially infested rabbits. Twenty five adult New Zealand white rabbits were used in this study. The rabbits were assigned randomly into five groups/ 5 rabbits in each group. Each rabbit was experimentally infested with 100 mites/ ear. The first group was designated the positive control group and was not treated. The second and third groups were treated with doramectin 200 and 400 μg/kg bw, respectively. Groups 4 and 5 were treated by dressing with ivermectin in one dose and 2 doses with a 1 week interval. After the therapy, all rabbits were examined microscopically on the 7th, 14th, and 28th day post treatment and the number of live mites (larvae, nymphs, and adults) on each rabbit was counted at the end of the experiment (28th day). The results showed that the rabbits treated subcutaneously with doramectin at a single dose of 200 μg /kg bw showed a very low effect, although there was significant improvement when the dose was doubled to 400 μg /kg bw, with the number of mites counted decreasing significantly. Rabbits treated topically with ivermectin spot-on, a single dose or 2 doses, showed great improvement of the lesion: the number of mites was reduced to zero. In conclusion, this work showed that ivermectin spot-on applied locally on infested ears proves to be more effective against P. cuniculi than doramectin injected subcutaneously. Further trials on ear mange therapeutics in rabbits are to be encouraged.

scholarly journals Promotion of Dental Pulp Wound Healing in New Zealand White Rabbits’ Teeth by Thai Propolis Product

2018 ◽  
Vol 36 (1) ◽  
pp. 17-24 ◽  
Author(s):  
Nattriya Likitpongpipat ◽  
Somboon Sangmaneedet ◽  
Poramaporn Klanrit ◽  
Rajda Noisombut ◽  
Suttichai Krisanaprakornkit ◽  
...  
Keyword(s):  
Wound Healing ◽  
New Zealand ◽  
Calcium Hydroxide ◽  
Median Number ◽  
Positive Control ◽  
Negative Control ◽  
Histological Evaluation ◽  
Dentinal Tubules ◽  
Pulp Capping ◽  
New Zealand White Rabbits

This study examined and compared wound healing between Thai propolis product and calcium hydroxide paste as pulp-capping agents after partial pulpotomy in New Zealand white rabbits. Forty incisor teeth from 10 rabbits were treated. Thirty-six teeth received class V cavity preparations with partial pulpotomy and application of either propolis or calcium hydroxide paste. Similar cavity preparations were performed in 2 teeth without any capping material as a positive control, whereas 2 teeth without the cavity preparation served as a negative control. Histological evaluation showed that both groups had dentin bridge formation. Dentinal tubules in the dentin bridge were more orderly arranged in the Thai propolis group than in the calcium hydroxide group. Wound healing and the median number of hyperemic blood vessels were not statistically significant different between the 2 groups. Thai propolis product may be used as a pulp-capping agent.

scholarly journals Efficacy of Dietary Supplementation with Capsicum Annum L on Performance, Hematology, Blood Biochemistry and Hepatic Antioxidant Status of Growing Rabbits

Animals ◽  
2020 ◽  
Vol 10 (11) ◽  
pp. 2045
Author(s):  
Hamada Elwan ◽  
Mostafa Abdelhakeam ◽  
Sally El-Shafei ◽  
Atef Abd El-Rahman ◽  
Zienhom Ismail ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
New Zealand ◽  
Dietary Supplementation ◽  
Basal Diet ◽  
Control Group ◽  
Spectrometric Analysis ◽  
Hot Pepper ◽  
Wide Range ◽  
New Zealand White Rabbits ◽  
Physiological And Biochemical

Animals fed with a high amount of a wide range of antioxidants in their diet are significantly protected against oxidative stress. Powerful antioxidant substances such as vitamin E, vitamin C, and carotenoids are present naturally in red-hot pepper (RHP). This study hypothesized that using RHP may provide protection against oxidative stress and enhance animal physiological responses. Thus, this study aimed to investigate the effect of feeding New Zealand white rabbits with RHP-supplemented diets on their physiological and biochemical responses. New Zealand White rabbits (age = 6 weeks, n = 48) were split equally into three groups (n = 16 in each group). One group was fed a basal diet only (control group), with the other two groups fed a basal diet along with 1 and 2% RHP. Mass spectrometric analysis for the RHP methanolic extract showed some phenolic compounds, such as p-coumaric, sinapinic acids, vanillic, and luteolin, as well as catechin and its isomers. Hepatic antioxidant enzymes (SOD, GSH, GSH-Px, and CAT) were significantly elevated (p < 0.05) by feeding rabbits diets supplemented with 1 or 2% RHP. The addition of RHP significantly enhanced immune-responses; phagocytic activity, chemotaxis, TIg, IgG, IgM, and IgA increased when growing rabbits were fed RHP compared with the control group. In conclusion, dietary supplementation of 1 or 2% RHP may play a role as an enhancer of growth and immune response in growing rabbits.

scholarly journals Model of a critical size defect in the New Zealand White rabbit’s tibia

2016 ◽  
Vol 15 (1) ◽  
Author(s):  
Che Nor Zarida Che Seman ◽  
Zamzuri Zakaria ◽  
Mohamed Azril Mohamed Amin ◽  
Mohd Shukrimi Awang ◽  
Nazri Mohd Yusof ◽  
...  
Keyword(s):  
New Zealand ◽  
Critical Size ◽  
Zealand White Rabbit ◽  
Control Group ◽  
Critical Size Defect ◽  
New Zealand White Rabbit ◽  
Surgical Suture ◽  
Study Results ◽  
Rabbit Tibia ◽  
New Zealand White Rabbits

Introduction: Critical size defects (CSD) in the long bones of New Zealand White rabbit (Oryctolagus cuniculus) have been used for years as an experimental model for investigation of the effectiveness of a new bone substitute materials. There are varieties of protocols available in the literature. This technical note attempts to present an alternative surgical technique of a CSD in the New Zealand white rabbit tibia. Methods: Thirty-nine New Zealand White rabbits were used in this study. A CSD of approximately 4.5 mm (width) X 9.0 mm (length) was surgically drilled at the proximal tibial metaphysis, approximately 1 cm from the knee joint. The surrounding of soft tissue was repositioned and sutured layer by layer with bio absorbable surgical suture. Povidone soaked gauze and bandage were used as a dressing. There is no control group used in this study. Results: This alternative method created good CSD with less bleeding from the muscle observed. No mortality on other surgical complications were observed within 6 weeks, 12 weeks and 24 weeks following surgery. Conclusions: A simple and safe method for performing critical size defect was demonstrated and recommended as an approach for surgery on New Zealand White rabbits.

The Effect of Glucocorticoids on the Expression of L-Selectin on Polymorphonuclear Leukocyte

Blood ◽  
1999 ◽  
Vol 93 (8) ◽  
pp. 2730-2737 ◽  
Author(s):  
Motohito Nakagawa ◽  
Gregory P. Bondy ◽  
Dan Waisman ◽  
Diane Minshall ◽  
James C. Hogg ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Single Dose ◽  
New Zealand ◽  
Polymorphonuclear Leukocyte ◽  
Glucocorticoid Treatment ◽  
Thymidine Analogue ◽  
New Zealand White Rabbits ◽  
Inflammatory Stimuli ◽  
Baseline Levels ◽  
Glucocorticoid Effect

Abstract When active bone marrow release is induced by inflammatory stimuli, it is associated with an increase in L-selectin expression on circulating polymorphonuclear leukocyte (PMN). This contrasts sharply with glucocorticoid-induced granulocytosis that is associated with decreased L-selectin expression on PMN. The present study was designed to determine if the reduced L-selectin expression observed after glucocorticoid treatment is the result of suppression of L-selectin synthesis in the bone marrow. New Zealand white rabbits treated with dexamethasone (2.0 mg/kg, a single dose intravenously) were shown to have decreased L-selectin expression on circulating PMN 12 to 24 hours after treatment (P &lt; .01) with a return to baseline levels by 48 hours. When dexamethasone was administered 48 hours after the bone marrow PMN were pulse labeled with the thymidine analogue, 5′-bromo-2′-deoxyuridine (BrdU), L-selectin expression on BrdU-labeled PMN released from the bone marrow was decreased (P&lt; .01). Dexamethasone decreased L-selectin expression on segmented PMN in the bone marrow (P &lt; .05) but not on PMN already in the circulation. We conclude that glucocorticoids decrease L-selectin expression on circulating PMN by downregulating L-selectin expression in the maturation pool of bone marrow and speculate that this is an important glucocorticoid effect that influences the recruitment of PMN into inflammatory sites.

In Vitro Effect of the Biomechanics After Photodynamic Therapy (PDT) Intervention on Rabbit Cornea

2021 ◽  
Author(s):  
Guanyu Su ◽  
Shaofeng Han ◽  
Bowen Song ◽  
Kai Cao ◽  
Qingfeng Liang
Keyword(s):  
New Zealand ◽  
Anterior Chamber ◽  
Toluidine Blue ◽  
Red Light ◽  
Tangent Modulus ◽  
Control Group ◽  
Rabbit Cornea ◽  
Corvis St ◽  
New Zealand White Rabbits

Abstract Objective: To observe the change of the biomechanical properties of rabbit cornea after the intervention by corneal collagen cross linking (CXL) and toluidine blue O combined with red light (TBOR).Methods: The study was carried out in compliance with the ARRIVE guidelines. 20 healthy adult New Zealand white rabbits were randomly divided into two groups. One group was taken with Riboflavin combined with UV light therapy in the right eye of healthy New Zealand white rabbits, and the other group was taken toluidine blue O combined with red light (TBOR) treatment. All left eyes were taken as controls. Parameters, from Pentacam and Corvis ST like K1 (The keratometry readings of the flattest), K2 (The keratometry readings of the steepest), ACD (Anterior chamber depth), Pupil diameter, ACA (Mean angle of the anterior chamber), ACV (Volume of the anterior chamber), bIOP (Biomechanical Intraocular pressure), CT (Corneal thickness, pachymetry), A1 Time(Time from starting until the first applanation), A1 Velocity(Corneal speed during the first applanation moment), A2 Time (Time from starting until the second applanation), A2 Velocity (Corneal speed during the second applanation moment), HC Time(Time from starting until highest concavity is reached), Peak Dist(Distance of the two “peaks” at highest concavity) and HC-Radius(The radius at highest concavity), were examined before and 2 weeks after intervention. The rabbits were sacrificed after anesthesia. Then, their corneas were removed for corneal stretch test.Results: With the examination of Pentacam and Corvis ST, IOP(11.28 ± 11.2mmHg v.s. 6.66 ± 4.02mmHg) and A1 time(7.03 ± 1.27s v.s. 6.55 ± 0.35s) were increased, comparing with those before intervention. From the in vitro corneal stretch test, the tangent modulus of the CXL group was more than 3 times of the Control group, whereas the tangent modulus of the TBOR group was about 0.7 times to that of the Control group.Conclusions: From the rabbit cornea intervention with CXL and TBOR, CXL showed an obvious effect of increasing the hardness of rabbit cornea, while TBOR may did no help to increase the hardness of cornea.

P4462Atorvastatin and fenofibrate exert opposite effects on the vascularization and characteristics of visceral adipose tissue

2019 ◽  
Vol 40 (Supplement_1) ◽  
Author(s):  
O Perez-Mendez ◽  
M Luna-Luna ◽  
A Mondragon-Garcia ◽  
A Aranda-Fraustro
Keyword(s):  
Gene Expression ◽  
Adipose Tissue ◽  
New Zealand ◽  
High Risk ◽  
Visceral Adipose Tissue ◽  
Control Group ◽  
Increased Risk ◽  
Tnf Α ◽  
New Zealand White Rabbits ◽  
Visceral Adipose

Abstract Background Statins may precipitate the onset of type 2 diabetes (T2D) in high-risk patients. In contrast, only the subset of individuals with insulin resistance (IR) and/or diabetes receives cardiovascular benefits with fibrates. The mechanism responsible of such effects may be related with visceral adipose tissue (VAT). In this context, previous observations have suggested that atorvastatin induced an increase of VAT whereas fenofibrate had the opposite effects in rabbits. Purpose To determine the mass, morphology and vascularization of VAT in New Zealand white rabbits that received of atorvastatin or fenofibrate during two months. Methods New Zealand white rabbits (n=6 per group) received by oral gavage during 2 months, 0.33 mg/kg of atorvastatin or 2.6 mg/kg fenofibrate. The control group received 0.5 mL of vehicle. Plasma lipids were monitored. The visceral adipose tissue (VAT) was dissected and quantified. The expression of genes related with vascularization, VEGF-A and TGF-β, FGF2 as well as TNF-α were determined by qPCR in VAT. Histological slices were stained by hematoxilin and eosin to determine the size of adipocytes. The marker of angiogenesis, PECAM-1, was determined by immunohistochemistry. Results As expected, the cholesterol from atorvastatin was lower after treatment while triglycerides decreased in fenofibrate group. The mass of VAT from fenofibrate group was 46% lower compared with the controls meanwhile atorvastatin was associated with a larger diameter of adipocytes (+65%) than that of the control and fenofibrate groups. FGF2 gene expression was lower in fenofibrate than in control group (−54%). By contrast, VEGF-A gene expression in fenofibrate-treated rabbits was 110% higher than in control group. TGF-β and TNF-α remained comparable among groups. In agreement with the gene expression, the marker of angiogenesis PECAM-1 was slightly but significantly higher (+10%) in rabbits treated with fenofibrate than in controls, as determined by immunohistochemistry. Conclusion Fenofibrate enhanced the VEGF-A gene expression, PCAM-1 in VAT whereas decreased its total mass. In contrast, atorvastatin increased the adipocyte size without any effect on vascularization markers. These results suggest that fenofibrate is associated with a favorable remodeling of VAT, in contrast with atorvastatin, which induced a non-favorable remodeling of VAT. These results may be related with the cardiovascular benefits of fenofibrate and the increased risk of T2D in high-risk subjects induced by atorvastatin.

Effects of low-dose ketanserin on atherosclerosis in rats and rabbits

2010 ◽  
Vol 88 (11) ◽  
pp. 1054-1060 ◽  
Author(s):  
Yong-Sheng Yu ◽  
He-Hui Xie ◽  
Ling Li ◽  
Shu-Wei Song ◽  
Ping Han ◽  
...  
Keyword(s):  
Blood Pressure ◽  
Vitamin D ◽  
New Zealand ◽  
Treatment Group ◽  
Low Dose ◽  
Control Group ◽  
High Cholesterol Diet ◽  
Cholesterol Diet ◽  
High Cholesterol ◽  
New Zealand White Rabbits

The present study was designed to test the hypothesis that a small dose of ketanserin, which enhances baroreflex activity, prevents the early lesions of atherosclerosis. In experiment 1, baroreflex sensitivity (BRS) was measured in 31 spontaneously hypertensive rats (SHRs) in a conscious state using a computerized blood pressure monitoring system. Four weeks later, the rats were administered vitamin D3 and fed a high-cholesterol diet for 8 weeks to induce atherosclerosis. Then their hearts and aortae were removed for pathological examination. A negative correlation was found between BRS and the scores of coronary (r = –0.460, P < 0.01) and aortic atherosclerosis (r = –0.448, P < 0.05) in SHR. In experiment 2, SHRs were divided into 3 groups (n = 10 in each group) and received a dose of ketanserin of 0.3, 1.0, and 3.0 mg/kg (i.g.), respectively. At the smallest dose (0.3 mg/kg), ketanserin did not lower blood pressure but enhanced BRS. In experiment 3, SHRs were administered vitamin D3, fed a high-cholesterol diet, and simultaneously treated with low-dose ketanserin. The atherosclerosis scores of the treatment group were significantly lower than those of the control group (coronary score: 0.90 ± 0.14 vs. 1.76 ± 0.27, P < 0.05; aortic scores: 1.00 ± 0.39 vs. 2.18 ± 0.41, P < 0.05). In experiment 4, male New Zealand White rabbits were fed a high-cholesterol diet and treated with low-dose ketanserin at the same time. The atherosclerosis scores of the treatment group were significantly lower than those of the control group (aortic scores: 0.26 ± 0.20 vs. 0.60 ± 0.31, P < 0.05). In conclusion, the present study demonstrated, for the first time, that low-dose ketanserin prevented the development of atherosclerosis independent of its blood pressure lowering action in SHRs and New Zealand White rabbits at least in part via enhancement of arterial baroreflex function.

Cricoid Quartersection vs. Anterior-Posterior Cricoid Split: Effect on Subglottic Growth in Rabbits

1991 ◽  
Vol 105 (6) ◽  
pp. 826-831 ◽  
Author(s):  
David A. Foggia ◽  
Steven D. Gray ◽  
Kevin M. Kelly
Keyword(s):  
New Zealand ◽  
Control Group ◽  
Cross Sectional ◽  
Split Effect ◽  
New Zealand White Rabbits ◽  
Anterior Posterior

Five-week-old New Zealand white rabbits were divided into three groups: control, anterior-posterior cricoid split with stent, and cricoid quartersection (anterior-posterior-lateral cricoid split) with stent. The stents were removed 2 weeks after placement. The rabbits were allowed to grow for six months, at which time they were killed and the larynges were studied. Average, minimal, and maximal subglottal cross-sectional areas were calculated for each animal and adjusted for weight. The cricoid quarter section group had significantly greater average, minimal, and maximal subglottal cross-sectional areas compared to controls and significantly greater minimal and maximal cross-sectional areas compared to the anterior-posterior cricoid split group. The anterior-posterior cricoid split group and the control group did not significantly differ with respect to any of the three parameters. The results indicate that anterior-posterior cricoid split and cricoid quartersection do not adversely affect subglottic growth. Cricoid quartersectioning was superior to the anterior-posterior cricoid split in its ability to expand subglottic airway dimension.

scholarly journals Effects of Zoledronic Acid on Physiologic Bone Remodeling of Condylar Part of TMJ: A Radiologic and Histomorphometric Examination in Rabbits

2014 ◽  
Vol 2014 ◽  
pp. 1-6 ◽  
Author(s):  
Ufuk Tatli ◽  
Yakup Üstün ◽  
Mehmet Kürkçü ◽  
Mehmet Emre Benlidayı
Keyword(s):  
Single Dose ◽  
Zoledronic Acid ◽  
Bone Remodeling ◽  
Infusion Pump ◽  
Rabbit Model ◽  
Mandibular Condyle ◽  
Control Group ◽  
New Zealand White Rabbits ◽  
Base Data ◽  
Experimental Group

Objective. The purpose of the present study is to evaluate the effects of systemically administered zoledronic acid (ZA) on the physiological bone remodeling and the microarchitectural parameters of the condylar part of TMJ in a rabbit model.Study Design. Thirty skeletally mature male New Zealand white rabbits were randomly divided into two groups. The experimental group was administered an intravenous, single dose of 0.1 mg/kg ZA diluted with 15 mL of saline in a 15-minute perfusion with an infusion pump. The control group was administered only saline infusion for 15 minutes. All rabbits were sacrificed on the 21st postoperative day. Radiodensitometric and histomorphometric examinations were performed on the harvested mandibular condyles. The data were analyzed statistically.Results. Radiodensitometric findings showed that ZA treatment resulted in a significant increase in the mineralization of mandibular condyle. This result was supported by the histomorphometric findings.Conclusion. The present study has revealed that a temporary delay in the physiological bone remodeling using single dose of ZA increases bone mineral content and makes the microarchitecture of the mandibular condyle more compact. These effects may be regarded as base data and considered in numerous clinical situations including TMJ.

scholarly journals Effect of formaldehyde inhalation on rabbit nasal mucosa: a light microscopic study—an animal model for inhalational irritants on nasal mucosa

2021 ◽  
Vol 37 (1) ◽  
Author(s):  
Wael Mohamed Adel AbdelKafy ◽  
Reham Farouk Zittoon ◽  
Ashraf Saad Abou-Halawa ◽  
Ereny Fekry Youssef Makary ◽  
Mohamed Rifaat Ahmed
Keyword(s):  
Animal Model ◽  
New Zealand ◽  
Nasal Mucosa ◽  
Empty Space ◽  
Inflammatory Cells ◽  
Nerve Fibers ◽  
Water Soluble ◽  
Control Group ◽  
New Zealand White Rabbits ◽  
Formaldehyde Inhalation

Abstract Background Formaldehyde is associated with many adverse health effects and is classified as a human carcinogen. Formaldehyde is highly water-soluble and readily absorbed and metabolized by the respiratory mucosa upon inhalation. The histopathological effects of formaldehyde on the nasal mucosa and olfactory nerves in adult New Zealand white rabbits were studied to validate this animal model of inhalational irritants. Results Compared to control group 1 (exposed to air), groups 2 and 3 (exposed to formaldehyde for 90 min and 210 min, respectively) exhibited disrupted nasal tissue, ulcerated epithelial coverings, markedly dilated blood vessels, and increased numbers of inflammatory cells in the lamina propria. The olfactory neuro-epithelium exhibited a reduction in the number of cilia. Many sustentacular cells lost their microvilli. Olfactory nerves exhibited nerve bundle shrinkage within the perineural sheath, leaving an empty space with evidence of edema within the nerve fibers. Conclusion Formaldehyde inhalation has destructive effects on the nasal mucosa and olfactory nerves in adult New Zealand white rabbits. These results validate the use of this animal model to assess the effects of inhalational irritants on the nasal mucosa.

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