scholarly journals Co-infection of Trichuris vulpis and Toxocara canis in different aged dogs: Influence on the haematological indices

2021 ◽  
Vol 29 (2) ◽  
pp. 129-134
Author(s):  
I. V. Saichenko ◽  
A. A. Antipov ◽  
T. I. Bakhur ◽  
L. V. Bezditko ◽  
S. S. Shmayun

Trichuris vulpis and Toxocara canis are worldwide parasitic nematodes affecting dogs and mammals of the Canine family. Due to the special structure of the shells, the eggs of these geohelminths can maintain their viability in soil, sand, water and the environment for a long time. The study involved young (6–12 months old) and adult (1.5–8.0 years old) dogs affected by co-infection of T. vulpis + T. canis; the control group consisted of dewormed healthy animals of the same age. Parasitological examination of dogs' faeces was performed using a "Counting Chamber for Ovoscopic Researches"; morphological parameters, indicators of nutrient metabolism, mineral metabolism and activity of enzymatic systems were determined in blood and serum. According to the results of parasitological research on the dogs, it was found that young animals are more prone to toxocarosis, and adults – trichurosis. The co-infection of nematodes T. vulpis + T. canis in dogs develops several changes in haematological parameters: a significant decrease in erythrocytes, haemoglobin, MCV, MCH, and haematocrit, leukocytosis with basophils and eosinophilia in young infected animals; and eosinophilia and basophilia (15.9 times) in adults, compared with healthy dogs of the same age. Among the changes in serum biochemical parameters, young infected dogs showed a decrease in the concentration of total protein and albumin content, an increase in the content of "acute phase" proteins (α-1, α-2 and β-globulins), an increase in cholesterol and total bilirubin; in adult infected dogs, a decrease in albumin content, an increase in the content of α-1, α-2, and β-globulins, an increase in the concentration of cholesterol and total bilirubin, a decrease in the concentration of urea in comparison with healthy animals were determined. Also, among the indicators of mineral metabolism, a decrease in the concentration of calcium and magnesium in the serum was found in young infected dogs. Among the changes in the enzyme metabolism in the serum of infected dogs, there was an increase in the activity of all studied enzymes in animals of both research groups: ALT, AST, α-amylase, GGT and ALP. Thus, co-infection with nematodes T. vulpis + T. canis has a complex pathogenic effect on the body of dogs of all ages, which manifested itself in multiple changes in haematological parameters. In the future, the authors’ team plan to develop comprehensive measures to combat nematode infections in different living conditions of dogs, taking into account the results of the current research.


2018 ◽  
Vol 12 (08) ◽  
pp. 666-672 ◽  
Author(s):  
Francesco La Torre ◽  
Angela Di Cesare ◽  
Giulia Simonato ◽  
Rudi Cassini ◽  
Donato Traversa ◽  
...  

Introduction: Dogs may act as potential sources of zoonotic parasites, e.g. intestinal helminths like Toxocara spp., Ancylostoma spp., Echinococcus spp. In particular circumstances, the environment contaminated by parasitic elements represents a source of infection for people and animals. The present study has evaluated the presence of zoonotic helminths in house dogs from central and north-eastern Italy. Methodology: Stool samples from 493 dogs were examined by a qualitative copromicroscopic technique and differences in prevalence of zoonotic parasites were statistically examined in relation to canine individual data. Results: 48/493 (9.7%) were positive for at least one parasite. Helminths recovered were Trichuris vulpis (5.5%), Toxocara canis (4.3%), Ancylostoma spp. (0.6%) and Eucoleus aerophilus (0.4%), while no cestodes were detected. Age and living with other dogs resulted risk factors for T. canis infection. Conclusions: The health risk associated with the occurrence of parasitic nematodes in privately owned dogs, along with the current anthelmintic treatment plans, are discussed.



2021 ◽  
Vol 12 (2) ◽  
pp. 289-293
Author(s):  
V. S. Sakara ◽  
A. Y. Melnyk ◽  
V. V. Sakhniuk ◽  
N. V. Vovkotrub ◽  
M. M. Fedorchenko ◽  
...  

Perosis is a common metabolic disease of industrial birds, especially broiler chickens. It leads to a violation of the balance of biotic substances in the body of chickens, which is clinically manifested by the curvature of the limbs, reduced mobility, and, consequently, reduced profitability of meat production. Prevention of perosis is possible provided that chickens receive a sufficient amount of manganese in a biologically available form. Studies were conducted to determine the efficiency of use of manganese chelates (pantothenate and lysinate) for prevention of perosis in broiler chickens. Efficacy was confirmed by examining changes in the clinical state, indicators of protein and mineral metabolism, as well as meat productivity of birds. For the experiment, broiler chickens of the Cobb-500 cross were taken at the age of 14 days. The birds of the control group received a standard diet, and the chickens from two experimental groups additionally received manganese pantothenate and lysinate with water during the critical period for the development of perosis – 14–28 days old. After 14 days of administration of manganese pantothenate and lysinate, the weight of the experimental birds at the age of 28 days was greater by 133.6 g (+11.0%) and 142.2 g (+11.7%), respectively, in comparison with poultry of the control group. Additional provision of manganese pantothenate and lysinate to chickens of the experimental groups contributed to an increase in the blood serum total protein concentration by 11.0% and 12.8 %, albumin – by 10.1% and 8.2%, magnesium – by 8.1% and 9.0% and manganese – by 29.6% and 26.9%, respectively, compared with indicies of the control group birds. The use of manganese chelates in the form of pantothenate (0.2 mL/L of water) and a lysinate (0.5 mL/L) during the 14–28th days of broiler chickens’ rearing provides 100% prevention of perosis. This reduces the death of broiler chickens, increases body weight, and, as a result, significantly increases the profitability of meat production.



2018 ◽  
Vol 20 (83) ◽  
pp. 320-324 ◽  
Author(s):  
N.V. Chеrniy ◽  
E.V. Matsenko ◽  
Yu.A. Shchepetilnikov ◽  
Yu. V. Maslak ◽  
O.S. Machula ◽  
...  

The aim of this study was to determine the effect of supplement «Press-Acid» on some parameters of mineral and protein metabolism and resistance of piglets. The study was done in condition «Meta» on two groups of piglets of the F-1 (BWхL) genotype. There were determined indexes of bacterial and lysocim activity of blood serum, calcium and phosphorus in piglets at the age of 21–30–45–75 days. Effect of supplement on the growth rate, morbidity and safety of pigs were revealed. We used hygienic, zootechnical, hematological, biochemical, immunological and statistical methods of analysis. The influence of «Press-Acid», which consist of formic, lactic, citric and fumaric acids, was established for the first time. The concentration of calcium in blood serum of experimental group of piglets were lower than in animals of control group. The level of phosphorus increased in blood serum of experimental animals on 11.83% (30 day), on 3.74% (45%), on 17.65% (75 day) respectively. Concentration of hemoglobin and the amount of erythrocytes increased on 11.8% and on 1.82% (30 day) in animals that feed the supplement. There were established the increase of viability of the pigs with gastro-intestinal diseases which were given the supplement in comparison with that, who did not feed it. Severity of diseases was determined using the Melenberg coefficient which did not higher than 1.2–2.4 score, it was lower on 6,0–9,3 unit than in control group. Using the supplement allowed to increase the intensity of growth of piglets by 3.7% (45 day) and 9.4% (75 day) respectively. Concentration of total protein, albumines and globulins increased on 8.66%, 9.90%, 7.3% respectively. The activity of alaninaminotransphaerasae in the blood serum of experimental animals were lower on 3.45% than in control piglets. Lysocym and bacterial activity of blood serum were higher in experimental group of piglets. Feeding piglets with addition supplement Press Acid promotes higher grow rates in experimental animals, that can be explained increases of oxidative-reducing processes in them organism. Feeding piglets with acidifier «Press-Acid», «Nifulin», zinc oxide, probiotics «Lactisan Complex» promotes growth energy, increase of morphological, biochemical and immunological parameters which indicates a higher level of oxidative reaction in the body. A low coefficient of Melenberg is recorded in the piglets at the age of 1 day. Safety was within 92.1–97.0%. That indicates a more successful elimination of complexes of protein, mineral substances, humoral protection in blood of experimental group of animals.



2021 ◽  
Vol 52 (4) ◽  
Author(s):  
Ernes Karić ◽  
Aida Hodžić ◽  
Amir Zahirović ◽  
Amina Hrković-Porobija ◽  
Husein Orhan

The exposure of the body to stress, regardless of whether it comes from physical, chemical or emotional stimuli from the environment, causes an inadequate adaptation of the organisms which can contribute to the development of various diseases. Abnormally high blood concentrations of cortisol, known as stress hormone, lead to the development of a hormonal disorder called hyperadrenocorticism or Cushing’s syndrome. In the majority of cases, Cushing’s syndrome is diagnosed when symptoms are apparent, and screening endocrinological test confirms the existence either of increased cortisol production or decreased sensitivity of the hypothalamic-pituitary-adrenal axis to negative glucocorticoid feedback. In our research, we examined a total of 23 male and 7 female dogs that were suspected to have Cushing’s syndrome, based on history and clinical signs. A total of 15 male and 5 female dogs were positive for Cushing’s syndrome (HAC group), whereas the remaining dogs were used to form non-HAC group. Using the apparatus IDEXX “Vet Test 8008”, the following biochemical parameters were determined: glucose, urea, creatinine, phosphorus, calcium, total protein, albumin, globulin, alanine aminotransferase, alkaline phosphatase, bilirubin, cholesterol, and amylase. Regarding haematological parameters, the following parameters were investigated: erythrocytes, leukocytes, platelets, erythrocyte indices (MCV, MCH, MCHC, RDW), white blood cell count, haemoglobin and haematocrit, using “Laser cite vet lab Station” (IDEXX). No significant differences in haematological and biochemical blood parameters were noticed between the HAC and the non-HAC group of dogs. However, dogs suffering from Cushing’s syndrome had a higher value in the number of erythrocytes compared to the control group. The finding that has to be payed attention to is the difference in platelet count between the control group of dogs and dogs suffering from Cushing’s syndrome.



2015 ◽  
Vol 3 (1) ◽  
pp. 46 ◽  
Author(s):  
Naim Alam ◽  
Md Islam ◽  
Matiar Howlader ◽  
Nasrin Lucky

<p>The effects of Oxyclozanide (Tremacid®) on some clinical (body weight) and haematological parameters (TEC, Hb, PCV, ESR and TLC) were determined in this study. Among 55 cattle, 10 were selected, which were suffering from fascioliasis and divided randomly into two groups. Each group was consisting of five cattle. One was treated with Tremacid® @ (15 mg/kg body weight). Other was kept as an infected control group. Before trials with Tremacid® initial body weight, EPG of liver fluke and hematological parameters were examined. During the experimental period the faecal samples, clinical and hematological parameters were examined on 7th, 14th, 21st and 28th days for the determination of effects of Tremacid®. A significant reduction of EPG count was found on 7th, 14th, 21st and 28th day of Tremacid® (61.87%, 71.22%, 76.98% and 84.53%) in a treated cattle group. The EPG of an untreated control group was significantly (p&lt;0.01) increased about 3.97%, 7.64%, 11.04% and 64.89% respectively. Total TEC was increased after treatment with Tremacid® and decreased in an untreated control group significantly (p&lt;0.01), Likewise, after treatment with Tremacid® Hb content and PCV were increased, and ESR was decreased. Total leukocyte count (TLC) was decreased and the body weight was increased after Tremacid® treatment in the group A. On the other hand, Hb content PCV and body weight was decreased significantly (p&lt;0.01) in the group B.</p>



2021 ◽  
Vol 5 (2) ◽  
pp. 43-54
Author(s):  
Saifa Nasar Trisha ◽  
Md Shafiqul Islam ◽  
Md Rakibul Hasan ◽  
Md Mahmudul Hasan Sikder ◽  
Most Shumi Akhter Sathi

The aim of this study was to evaluate the effects of discriminate and indiscriminate use of colistin sulfate on body weight and haematological parameters in broiler. Day-old-broiler chicks were collected and reared for up to 31 days. The chicks were randomly divided into control, discriminate and indiscriminate antibiotic group. The discriminate group was treated with colistin sulfate antibiotic (Eskolis-24) for one week (from 16th to 22th day) followed by one week withdrawal period. In case of indiscriminate group, the poultry were treated with colistin sulfate antibiotic for 14 days (from 16th to 29th day) without any withdrawal period until sacrifice. The body weight of the birds were recorded daily. The mean body weight was highest in indiscriminate group (1408.22±133.49 gm) followed by discriminate group (1330.15±134.93 gm) and control group (1243.81±173.19 gm). The differences among means of three groups were not statistically significant. The Total erythrocyte count (Million/mm3) of control, discriminate, and indiscriminate groups were 2.53± 0.05, 2.56±0.08, and 2.52±0.08 respectively. The hemoglobin (gm%) were 7.06± 0.09, 7.03± 0.12, and 7.11±0.09 respectively and the Packed Cell Volume (%) were 19.66±1.11, 19.33±0.95, and 20.16±0.70 respectively. The results found were not statistically significant among the groups for TEC, Hb and PCV respectively. Total lymphocyte (%) count were 67.16±0.60, 70±1.15, and 71.83±1.30 respectively. Total neutrophil (%) count were 32.83± 0.60, 28.83±1.42, and 26.83±1.10 respectively. Total eosinophil (%) count were 0± 0, 1±0.44, and 1.16±0.40 respectively. Total basophil (%) count were zero (0) and total monocyte (%) count were 0± 0, 0.17±0.17, and 0.17±0.17 respectively. In our experiment, both discriminate and indiscriminate groups showed no significant differences of lymphocyte, neutrophil, eosinophil, basophil and monocyte. Therefore, discriminate and indiscriminate use of colistin sulfate for two weeks has no effect on haematological parameters of broiler poultry. Further experiment needed for more concise conclusion. Asian Australas. J. Food Saf. Secur. 2021, 5 (2), 43-54



2021 ◽  
Vol 6 (2) ◽  
pp. 94-100
Author(s):  
V. M. Likhman ◽  
◽  
A. A. Merkulov ◽  
A. N. Shevchenko ◽  
S. V. Tkach ◽  
...  

The relevance of the emergence of innovative technologies, more sophisticated diagnostics methods, opportunities for intensive therapy, antibacterial prophylaxis, surgical methods of treatment involving minimally invasive surgery, does not solve the problem of high overall mortality in severe acute pancreatitis at this time. This indicator for the last ten years remains at a high level (10-30%), and in case of inflammatory forms of severe pancreatitis, reaches 85%. Traditional laboratory criteria for the diagnosis of pancreatic necrosis may involve leukocytosis with the destruction of the leukocyte formula and appearance of myelocytes and metamylocytes in it; improvement of leukocyte index of intoxication; improvement of the nuclear index of the destruction; blood glucose growth; growth of blood urea; growth of neutrophil-lymphocytic coefficient; change in endogenous creatinine clearance; improvement of blood transaminases. The indicator enzymes have the greatest value for the diagnosis of gastrointestinal pancreatitis in the diagnostic plan. The determining of α-amylase activity is a sensitive and valuable criterion for diagnostics of gastrointestinal pancreatitis and control over the effectiveness of treatment. Also, talking about laboratory diagnostics of acute pancreatitis it is worth noting about acute-phase proteins. The increase in the level of acute-phase proteins in inflammation is a compensatory reaction associated with the ability to inhibit cells released during destruction and cause secondary tissue damage by proteolytic enzymes, as well as to suppress autoimmune aggression. Due to this, the inflammatory process in the body is limited and autoaggression is restrained. The purpose of the study was to study the proteins of the acute phase in the blood of patients with acute pancreatitis of varying severity. Materials and methods. The study is based on the results of examination of 88 patients with acute pancreatitis of varying severity who were treated at the V.T. Zaytsev Institute of the General and Urgent Surgery of Academy of Medical Sciences of Ukraine, Kharkiv, as well as 45 donors (control group). Results and discussion. The determination of the activity of serum enzymes in the dynamics of acute destructive pancreatitis was carried out. The parameters were verified before the operation and after the operative treatment in dynamics, as well as before and after the second operation in the dynamics. Markers of tissue destruction can simultaneously serve as indicators of the severity of the inflammatory process in the pancreas and determine the quality of the performed surgical intervention. The diagnostic value of ferritin and lactofferin as markers of an acute destructive process was proved on the basis of a significant increase in their level in blood serum and exudates of patients. It has been shown that ferroproteins are reliable markers of the inflammatory process, among others, including classical acute phase proteins. At the same time, their identification is distinguished by its explicitness in the diagnosis of not only latent destructive states. Conclusion. The detected changes in the concentration of clinical and laboratory parameters of alpha-2-macroglobulin, lactoferrin, C-reactive protein, ferritin in the blood serum correlate with the degree of the inflammatory process and purulent-destructive changes in the pancreas



Author(s):  
L.A. Nikanova ◽  
◽  
E.N. Kolodina ◽  
R.A. Rykov ◽  
◽  
...  

This article presents the results of studies conducted on piglets with the inclusion of natural feed additives in the diet in order to prevent and correct metabolism. The first experimental group of piglets received a total diet consisting of fullfledged compound feed, a complex micro-feed product consisting of blue-green algae Spirulina, dihydroquercetin and organic iodine, the second experimental group additionally received Spirulina and dihydroquercetin. The introduction of these natural feed additives into the diet of piglets helped to increase the adaptive ability , correction and prevention of metabolic disorders in the body. In these studies, dacha feed supplements prevented hyperbilirubinemia. As a result, the concentration of total bilirubin in the blood serum of pigs of the first experimental group was 1% lower, in the second experimental group it was 13,7% lower than in the control group. They also had a positive effect on the functional state of the liver, as evidenced by a lower content of AlAT and AsAT activity in the blood serum compared to those in piglets of the control group with a close cholesterol-forming fraction. The animals of the experimental group were less ill and the safety of this group was 100%, in the control group it was 90%. The average daily increase in live weight of pigs for 90 days in the first experimental group was 1% higher than in the control group, and in the second experimental group it was 21,7% higher.



2019 ◽  
Vol 21 (93) ◽  
pp. 60-64
Author(s):  
A. G. Pashchenko ◽  
I. I. Kovalchuk ◽  
R. S. Fedoruk

The inadequacy of mineral nutrition leads to inhibition of physiological and metabolic reactions in the body of honeybees. It is known that Cobalt chloride is used to activate oviposition of the queen bee. It was established that Cobalt and Nickel citrate, obtained by the method of nanotechnology, corrects the mineral metabolism and affects the metabolism of bees. It is known that Cobalt plays an important role in the work of enzymes; synthesis of vitamin B12, promotes assimilation of vitamins A, E, C; increases protein metabolism, participates in hematopoiesis. Nickel also has a pronounced effect on hemopoiesis, namely on the morphological composition of blood. But its effect on the cell and subcellular level is not well understood. The results of studies of the effect of soybean flour with the addition of Cobalt and Nickel citrates on the content of mineral elements in the tissues of the body of bees and honeycombs are given. The research was carried out in the farms in the Lviv region, in April-May at the bees of the Carpathian breed. It was established that the content of Ferrum, Cuprum and Germanium in the tissues of bees increases with the addition of soybean flour with Сobalt citrate at a dose of 2 mg per 500 g of soy flour. When Nickel citrate was added to the feed at a dose of 1 mg per 500 g of soy flour, the content of Ferrum and Cuprum increased in honeycombs. The complex combination of Citrits Co and Ni, soy flour with sugar syrup was characterized by a decrease in the level of Zn in the tissues of the bees compared to its contents in the control group samples. In samples of biological material, the content of Сobalt, Nickel and the essential elements Cuprum, Zink, Ferrum, Selenium, Germanium was determined by atomic emission spectrometry with inductively coupled plasma. Citrates of the microelements were produced by the method of M. Kosinov and V. Kaplunenkо.



Author(s):  
M. Nischemenko ◽  
V. Kaplunenko ◽  
V. KOZIY ◽  
O. Poroshinska ◽  
L. Stovbetska ◽  
...  

The article presents the results of the study of the influence of nanoacquahelats of biogenic and biocidal metals Selenium and Zinc together with vitamin E, on the mineral metabolism indexes of hens of Loman Brown breed. It is known that the inflow of mineral elements such as Calcium and inorganic Phosphorus into the body and its proper usage is essential for the maintenance of egg productivity and egg quality. In this regard, it is promising to study the changes of the above-mentioned processes with the Selenium, Zinc and vitamin E nanoacqualates supplementation. It has been established that the feeding of Selenium and Zinc nanocarboxylic acids with vitamin E positively affects the exchange of Calcium and inorganic Phosphorus in the laying hens. In particular, the content of calcium in the serum of tested hens was significantly higher (17.3-26.7%), on the 60th and 90th days of the experiment, comparing with the control group. The level of inorganic phosphorus, on the 60th and 90th days of the experiment, also increased by 17.7% and 16.9%, respectively, in experimental group of layer hens compared to control one. It is known that macroelements Ca and inorganic Phosphorus are important components for the organism of animals and, especially, birds. These minerals have no nutritional value, are not used by the body as a source of energy, but their role as structural elements cannot be overestimated. Both Calcium and inorganic Phosphorus are part of almost all organs and tissues. The growth and development of the organism, as well as its productivity depends on the presence of these elements in the diet. In addition, Calcium and inorganic Phosphorus are actively involved in the regulation of a number of vital functions. In the birds, it is the synthesis of proteins and the intense formation of egg minerals. They are also necessary for the further formation of bone tissue of the embryo. Consequently, the presence of these minerals in the diet and in the body of layers hens, to a large extent determine their weight and quality of eggs. The obtained results show the use of nanoacquahelats of Selenium and Zinc together with vitamin E stimulate phosphorus-calcium metabolism and alkaline phosphatase activity due to their biophysical properties which are based on activation of many biochemical processes according to the effect of Borisevich-Kaplunenko-Kosinov. Key words: Calcium, Phosphorus, laying hens, Selenium, Zinc, vitamin E.



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