scholarly journals Judicious and non-judicious use of colistin sulfate in indoor poultry experimentation and its effect on haematological parameters and body weight in broiler

2021 ◽  
Vol 5 (2) ◽  
pp. 43-54
Author(s):  
Saifa Nasar Trisha ◽  
Md Shafiqul Islam ◽  
Md Rakibul Hasan ◽  
Md Mahmudul Hasan Sikder ◽  
Most Shumi Akhter Sathi
Keyword(s):  
Body Weight ◽  
The Body ◽  
Haematological Parameters ◽  
Control Group ◽  
Broiler Chicks ◽  
Monocyte Count ◽  
Withdrawal Period ◽  
Colistin Sulfate ◽  
Antibiotic Group ◽  
And Control

The aim of this study was to evaluate the effects of discriminate and indiscriminate use of colistin sulfate on body weight and haematological parameters in broiler. Day-old-broiler chicks were collected and reared for up to 31 days. The chicks were randomly divided into control, discriminate and indiscriminate antibiotic group. The discriminate group was treated with colistin sulfate antibiotic (Eskolis-24) for one week (from 16th to 22th day) followed by one week withdrawal period. In case of indiscriminate group, the poultry were treated with colistin sulfate antibiotic for 14 days (from 16th to 29th day) without any withdrawal period until sacrifice. The body weight of the birds were recorded daily. The mean body weight was highest in indiscriminate group (1408.22±133.49 gm) followed by discriminate group (1330.15±134.93 gm) and control group (1243.81±173.19 gm). The differences among means of three groups were not statistically significant. The Total erythrocyte count (Million/mm3) of control, discriminate, and indiscriminate groups were 2.53± 0.05, 2.56±0.08, and 2.52±0.08 respectively. The hemoglobin (gm%) were 7.06± 0.09, 7.03± 0.12, and 7.11±0.09 respectively and the Packed Cell Volume (%) were 19.66±1.11, 19.33±0.95, and 20.16±0.70 respectively. The results found were not statistically significant among the groups for TEC, Hb and PCV respectively. Total lymphocyte (%) count were 67.16±0.60, 70±1.15, and 71.83±1.30 respectively. Total neutrophil (%) count were 32.83± 0.60, 28.83±1.42, and 26.83±1.10 respectively. Total eosinophil (%) count were 0± 0, 1±0.44, and 1.16±0.40 respectively. Total basophil (%) count were zero (0) and total monocyte (%) count were 0± 0, 0.17±0.17, and 0.17±0.17 respectively. In our experiment, both discriminate and indiscriminate groups showed no significant differences of lymphocyte, neutrophil, eosinophil, basophil and monocyte. Therefore, discriminate and indiscriminate use of colistin sulfate for two weeks has no effect on haematological parameters of broiler poultry. Further experiment needed for more concise conclusion. Asian Australas. J. Food Saf. Secur. 2021, 5 (2), 43-54

scholarly journals Thin layer chromatographic detection of colistin sulfate antibiotic residues in poultry tissues

2021 ◽  
Vol 7 (1) ◽  
pp. 01-08
Author(s):  
Md. Nazmul Hasan ◽  
Md. Shafiqul Islam ◽  
Md. Rakibul Hasan ◽  
Kazi Rafiqul Islam
Keyword(s):  
Thin Layer ◽  
The Body ◽  
Control Group ◽  
Human Consumption ◽  
Broiler Chicks ◽  
Antibiotic Residues ◽  
Withdrawal Period ◽  
Liver Sample ◽  
Colistin Sulfate ◽  
Antibiotic Group

Residues of antibiotic drugs in food and food products have been received much attention in recent years because of growing food safety concerns for public health. There are serious effects of antibiotic residues in meat for human consumption (e.g., increasing antimicrobial resistance, carcinogenicity, mutagenicity, and hypersensitivity). In this study, we used broiler chicks as a laboratory animal to detect the colistin sulfate antibiotic residue for human health concerns. The day-old broiler chicks were collected and reared for up to 31 days. The treatment was started from the day 16th until sacrifice. The chicks were randomly divided into three groups namely control group (Group A), discriminate antibiotic group (Group B) and indiscriminate antibiotic group (Group C) on the 14th day. The discriminate group was treated with an antibiotic, colistin sulfate maintaining the withdrawal period of one week. In case of indiscriminate group the withdrawal period was not maintained and antibiotic treatment was continued till the day of sacrifice. The body weights of the birds were recorded daily. The mean body weight was highest in indiscriminate group (1261.15±16.37gm) followed by discriminate group (1156.15±18.23gm) and the lowest was in control group (1008.49±18.11gm). The differences among mean weight gain were statistically significant (P<0.05) in antibiotic treated group compared with control group. The Thin Layer Chromatography revealed that all the samples were positive in indiscriminate group and in case of Liver, Kidney and Spleen, it was 100%. In case of discriminate antibiotic group all the samples were positive except thigh and breast muscle and the percentage was highest in Liver sample (66.67%). There was no positive sample in control group. The results were statistically significant (P<0.05). From the above findings, this research could be considered a need based research in Bangladesh to ascertain the influential effect of antibiotic abuse in poultry industry.

scholarly journals Effects of colistin sulfate on hematological parameters in broiler

2021 ◽  
Vol 7 (2) ◽  
pp. 113-117
Author(s):  
Md Nazmul Hasan ◽  
Md Shafiqul Islam ◽  
Md Rakibul Hasan ◽  
Kazi Rafiqul Islam
Keyword(s):  
Hematological Parameters ◽  
Blood Parameters ◽  
Control Group ◽  
Broiler Chicks ◽  
Withdrawal Period ◽  
Colistin Sulfate ◽  
Significant Difference ◽  
Antibiotic Group ◽  
Group A ◽  
Group B

Hematological indication is an important finding for pathophysiological analysis of biological science. This study was designed with the aim to investigate the effects of residual antibiotics on hematological parameters of broiler following discriminate and indiscriminate use. The day old broiler chicks were collected and reared up to 31 days. The treatment was started from the day 16th until sacrifice. The chicks were randomly divided into three groups namely control group (Group A), discriminate antibiotic group (Group B) and indiscriminate antibiotic group (Group C) on the 14th day. The discriminate group was treated with antibiotic, colistin sulfate maintaining the withdrawal period of one week. In case of indiscriminate group the withdrawal period was not maintained and antibiotic treatment was continued till the day of sacrifice. The Hb (%) of control, discriminate and indiscriminate groups were 8.34±0.15, 6.89±0.27, and 7.14±0.23 respectively. The differences among means were statistically significant (P<0.05). The Packed Cell Volume (PCV) of control, discriminate, and indiscriminate groups were 23.51±0.76, 21.17±0.94, and 22.83±1.19 respectively. The differences among means were statistically significant. The multiple pair wise comparison of means of blood parameters revealed that there was no significant difference among discriminate and indiscriminate groups. From the above findings, this research could be considered a need based research in Bangladesh to ascertain the influential effect of antibiotic on hematological parameters in broiler. Asian J. Med. Biol. Res. 2021, 7 (2), 113-117

scholarly journals Judicious and non-judicious use of cephalexin antibiotic in indoor poultry experimentation and detection of cephalexin residues in edible poultry tissues

2021 ◽  
Vol 9 (3) ◽  
pp. 288-296
Author(s):  
DM Nazmul Hossain ◽  
Md Shafiqul Islam ◽  
Md Rakibul Hasan ◽  
Md Anaruzzaman ◽  
Kazi Rafiqul Islam
Keyword(s):  
Body Weight ◽  
Hematological Parameters ◽  
The Body ◽  
Thigh Muscle ◽  
Control Group ◽  
Broiler Chicks ◽  
Significant Difference ◽  
Antibiotic Group ◽  
Group B ◽  
Poultry Tissues

Antibiotics are the most widely injudiciously used drugs for therapeutic purposes throughout the world. This study aimed to detect cephalexin residue after discriminate and indiscriminate administration of cephalexin antibiotic in broiler. Eighteen broiler chicks, DOC (Cobb-500) were collected & reared for up to 31 days. The chicks were randomly divided into three groups namely control group (Group-A), discriminate cephalexin group (Group-B) and indiscriminate cephalexin group (Group-C). Each group consists of 6 birds. In this investigation, only a detectable level of antibiotic residues was found in indiscriminate group of poultry. A total of 72 samples were investigated by the TLC method. The overall residue of cephalexin found in indiscriminate group as in liver (100%), kidney (83.33%), thigh muscle (25.00%), breast muscle (8.33%), fat and spleen (66.67%) respectively. The final average body weight were recorded highest in indiscriminate antibiotic group (1804.17 ± 73.92gm) followed by discriminate group (1741.33 ± 63.63gm) and control group (1453.33 ± 26.39gm). The body weight was found significantly (p<0.05) higher in the antibiotic treated groups. The hematological parameters such as Hb, PCV and TEC values of treatment groups showed significantly (p<0.05) lower, while PCV did not show any significant difference compared to the control group. Overall, the present study documented the widespread abuse of cephalexin and failure to implement the recommended withdrawal period will undeniably lead to deposition of residues in broiler tissues.

Significance of graded doses of aqueous seed extract of Moringa oleifera (L) on live body weight, gonadal, extragonadal dimensions and sperm reserves of Yankasa rams

2021 ◽  
pp. 33-40
Keyword(s):  
Body Weight ◽  
Moringa Oleifera ◽  
Seed Extract ◽  
The Body ◽  
Control Group ◽  
Live Body Weight ◽  
And Control ◽  
Standard Techniques ◽  
Different Doses ◽  
Apparently Healthy

The aim of the study was to investigate the effects of Moringa oleifera aqueous seed extract on live body weight, gonadal and extragonadal dimensions and sperm reserves of Yankasa rams. Twenty five apparently healthy Yankasa rams aged 1-2 years and weighing 19.0 ± 2.1 Kg were used for the study. The rams were randomly selected into five groups: A, B, C, D and E with five rams in each group as treatment and control groups respectively. Groups A - D were given oral dose of Moringa oleifera aqueous seed extract at a dose rate of 1000, 2000, 3000, 4000 (mg/kg), respectively while group E was given 10 ml/kg water orally, daily for five months. Live body weight, gonadal and extragonadal reserves were determined according to standard techniques. The results showed a significant increase in live body weight in the months of April to June among rams treated with different doses of Moringa oleifera aqueous seed extract compared with the control group. The control group showed no significant differences in the body weight, gonadal and extragonadal dimensions and sperm reserves. In conclusion, the treatment of Yankasa rams with Moringa oleifera aqueous seed extract increased live body weight, but had no significant effects on gonadal and extragonadal dimensions and sperm reserves in Yankasa rams. Therefore, it is recommended that M. oleifera aqueous seed extract can be used at doses of 2000mg/kg to 3000mg/kg in Yankasa rams for optimum gain in live body weight.

scholarly journals Administration of garlic and neem in broiler diet for safe meat production

2020 ◽  
Vol 48 (2) ◽  
pp. 116-126
Author(s):  
R Islam ◽  
MM Hossain ◽  
F Nargis ◽  
ME Hossain
Keyword(s):  
Body Weight ◽  
Basal Diet ◽  
Feed Additives ◽  
The Body ◽  
The Other ◽  
Control Group ◽  
Meat Production ◽  
Broiler Chicks ◽  
Treatment Groups ◽  
Broiler Production

This study was conducted to observe the effect of different administration method of garlic and neem on growth performances, feed efficiency and serum biochemical parameters for safe broiler production. The experiment was done for a period of 32 days with 384 one-day-old straight run broiler chicks. The broiler chicks were divided into six groups each of 64 birds and replicated to four subgroups each of 16 birds. The dietary groups were; control (basal diet; no additives), antibiotic (basal diet + antibiotic), garlic in feed (basal diet + 0.25% garlic powder), garlic in water (basal diet + garlic extracts), neem in feed (basal diet + 0.25% neem powder) and neem in water (basal diet + neem extracts). Results showed that the body weight and body weight gain increased significantly (P<0.05) in both the garlic and neem groups compared to the control group. Feed intake was not different (P>0.05) among the treatment groups. Garlic in feed and water and neem in feed groups showed better FCR (P<0.05) compared to the other treatment groups. There were no significant (P>0.05) difference in meat yield, bone development and dressing parameters except head and gizzard in different dietary groups. Supplementation of garlic with feed and water significantly (P<0.05) decrease cholesterol, triglyceride and LDL compared to the other groups. However, both the garlic and neem groups showed numerically higher HDL compare to the control group. There were no 0significant (P>0.05) differences in serum glucose and GPT among different treatment groups. GOT was significantly (P<0.05) lower in neem groups than that of garlic and antibiotic groups. Additive groups showed higher profitability than control group. Based on the results of the study, it may be suggested that the garlic and neem could be used both in feed and water as potential feed additives for safe broiler production. Bang. J. Anim. Sci. 2019. 48 (2): 116-126

scholarly journals Investigation of discriminate and indiscriminate use of doxycycline in broiler: an indoor research on antibiotic doxycycline residue study in edible poultry tissue

2020 ◽  
Vol 6 (1) ◽  
pp. 1-7 ◽  
Author(s):  
Md Rostom Ali ◽  
Md Mahmudul Hasan Sikder ◽  
Md Shakil Islam ◽  
Md Shafiqul Islam
Keyword(s):  
Breast Muscle ◽  
Thigh Muscle ◽  
Control Group ◽  
Broiler Chicks ◽  
Withdrawal Period ◽  
Antibiotic Group ◽  
Group A ◽  
Group B ◽  
Tlc Analysis ◽  
Residue Study

Abuse of antibiotics is more common in developing countries including Bangladesh. The aim of this study was to detect doxycycline residue after discriminate and indiscriminate administration in broiler poultry. Eighteen broiler chicks, DOC (Cobb-500) was collected & reared up to 31 days. On day 16, they were randomly divided into 3 groups, namely group–A (Control group), group-B (Discriminate group) and group-C (Indiscriminate group). Each group contains 6 birds. The discriminate and indiscriminate groups were treated with antibiotic, doxycycline. In group-B withdrawal period was followed by treatment for 7 days. On the other hand, withdrawal period was not maintained in group-C, i.e. the antibiotic treatment was continued until the day of sacrifice. TLC analysis revealed that intensity of doxycycline in discriminate antibiotic group for liver, kidney, thigh muscle and breast muscle samples were found 63.33%, 65.33%, 22.67% and 26.00% respectively. And in indiscriminate antibiotic group intensity of doxycycline for liver, kidney, spleen, thigh muscle and breast muscle samples were found 50.67%, 50.33%, 39.50% and 48.17% respectively. All the samples of control group were found 0% intensity. The different among intensity were statistically significant. Overall, the present study documented the widespread abuse of doxycycline and failure to implement the recommended withdrawal period will undeniably leads to deposition of residues in broiler tissues. Asian J. Med. Biol. Res. March 2020, 6(1): 1-7

scholarly journals Effects of oxyclozanide (Tremacid®) preparation against fascioliasis on clinical and haematological parameters in cattle of Bangladesh

2015 ◽  
Vol 3 (1) ◽  
pp. 46 ◽  
Author(s):  
Naim Alam ◽  
Md Islam ◽  
Matiar Howlader ◽  
Nasrin Lucky
Keyword(s):  
Body Weight ◽  
Untreated Control ◽  
Hematological Parameters ◽  
Experimental Period ◽  
The Body ◽  
Haematological Parameters ◽  
Total Leukocyte Count ◽  
Control Group ◽  
Untreated Control Group ◽  
Group B

<p>The effects of Oxyclozanide (Tremacid®) on some clinical (body weight) and haematological parameters (TEC, Hb, PCV, ESR and TLC) were determined in this study. Among 55 cattle, 10 were selected, which were suffering from fascioliasis and divided randomly into two groups. Each group was consisting of five cattle. One was treated with Tremacid® @ (15 mg/kg body weight). Other was kept as an infected control group. Before trials with Tremacid® initial body weight, EPG of liver fluke and hematological parameters were examined. During the experimental period the faecal samples, clinical and hematological parameters were examined on 7th, 14th, 21st and 28th days for the determination of effects of Tremacid®. A significant reduction of EPG count was found on 7th, 14th, 21st and 28th day of Tremacid® (61.87%, 71.22%, 76.98% and 84.53%) in a treated cattle group. The EPG of an untreated control group was significantly (p&lt;0.01) increased about 3.97%, 7.64%, 11.04% and 64.89% respectively. Total TEC was increased after treatment with Tremacid® and decreased in an untreated control group significantly (p&lt;0.01), Likewise, after treatment with Tremacid® Hb content and PCV were increased, and ESR was decreased. Total leukocyte count (TLC) was decreased and the body weight was increased after Tremacid® treatment in the group A. On the other hand, Hb content PCV and body weight was decreased significantly (p&lt;0.01) in the group B.</p>

scholarly journals Doxorubicin Deteriorates the Histomorphology of the Kidneys of Albino Rats

2017 ◽  
Vol 15 (1) ◽  
pp. 40-43
Author(s):  
Anup Pandeya ◽  
Chandra Bhushan Jha ◽  
Smriti Karki ◽  
Gajendra Prashad Rauniar
Keyword(s):  
Body Weight ◽  
The Body ◽  
Female Rats ◽  
Control Group ◽  
Albino Rats ◽  
Final Body Weight ◽  
Histological Features ◽  
Male And Female Rats ◽  
Significant Difference ◽  
And Control

Background and Objectives: Nephrotoxicity is one of the limiting factors for using doxorubicin as an anticancer chemotherapeutic. Reactive oxygen species and cytokines have been implicated in the nephrotoxicity induced by doxorubicin. The main objective of the present study is to identify and compare the histomorphological features in kidneys of albino rats and gross morphological features such as weight of rats and weight of the kidneys due to administration of doxorubicin. Materials and Methods: In the study, albino rats were taken as the animal model. Sixty animals were taken as the sample size. They were divided into two equal groups: experimental (n=30) and control (n=30). Rats of experimental group were treated with anticancer drug doxorubicin at a single intraperitoneal dose of 10 mg/kg body weight while the Control group of rats received a similar volume of 0.9% normal saline. The ethical clearance was taken prior to the research from IERB committee BPKIHS Dharan.  Results: Our results showed that there was high effect of drug in experimental groups of rats. It was seen that there was significant decrease in the body weight and weight of kidneys. The final body weight and kidney weight between experimental and control group showed the significant difference. Similarly there were no significant differences in the normal architecture between the male and female rats. The normal renal histological features were seen on the kidneys in the control group whereas the rats intervened with the drug had some disrupted histological features which reveal the toxicity of the drugs in the kidneys. Conclusion: The study showed toxicity of the drug in the kidneys of experimental groups of rats irrespective of gender and suggest that doxorubicin causes significant loss of the body weight and weight of kidneys and causes the disruption in the normal histological features.

scholarly journals Pengaruh Pemijatan terhadap Pertumbuhan dan Perkembangan Bayi Umur 3 – 4 Bulan di Wilayah Kerja Puskesmas Kanjilo Kecamatan Barombong Kabupaten Gowa

2019 ◽  
Vol 1 (1) ◽  
pp. 1-14
Author(s):  
Anna Sari Dewi
Keyword(s):  
Body Weight ◽  
Weight Gain ◽  
Body Weight Gain ◽  
Research Result ◽  
The Body ◽  
Rank Test ◽  
Control Group ◽  
Significance Level ◽  
Development Indicator ◽  
And Control

The research aimed at investigating whether there was the massage effect on the growth and development of the infants of 3 – 4 months old in the working area of PHC Kanjilo, Barombong District, Gowa Regency.  This was a quasy experiment research with the pretest-posttest method on the treatment and control groups. Sample consisted of 38 subjects who were selected by the purposive sampling technique. The samples were divided into 2 group, namely the treatment group ( n=19) and control group (n=19) by matching the same age and sex in the two groups. The variables measured were the body weight gain, height gain, development indicator change. The research used the statistic test of Wilcoxon signed rank test, Mann Whitney U test, McNemar test with the  significance level of 0.05. The research result indicates that after the massage has been carried out for 30 days, there is the significant change particularly on the body weight gain variable with the  value of p = 0,008 (<0.05) and the development indicator change variable with the value of p = 0.05.  While for the height, the change is not significant between the infants who are given the massage and are not given the massage with the value of p = 0,091 (> 0.05), likewise, the development indicator change is not significant on the infants who are not massaged (p=0,317).

Body Weight and Blood Biochemical Changes Following Nutritional Supplementation in Prepubertal Jaffrabadi Buffalo (Bubalus bubalis) Heifers

2019 ◽  
Vol 15 (01) ◽  
pp. 50-54
Author(s):  
R J Raval1 ◽  
K B Vala ◽  
V A Kalariya ◽  
A J Dhami ◽  
F S Kavani
Keyword(s):  
Body Weight ◽  
Bubalus Bubalis ◽  
Biochemical Changes ◽  
The Body ◽  
Nutritional Supplementation ◽  
Monthly Interval ◽  
Control Group ◽  
Control Groups ◽  
Blood Biochemical ◽  
And Control

The high plan of nutrition right from an early age is believed to enhance ovarian activity and early onset of puberty in heifers. This study was therefore conducted to know the body weight, ovarian and blood biochemical changes in nutritionally supplemented and control groups of prepubertal Jaffrabadi buffalo (Bubalus bubalis) heifers. A total of 12 animals of 30 ± 2 month's age and the identical body weight of 380–410 kg from Cattle Breeding Farm, JAU, Junagadh were utilized for this study. They were randomly divided into two equal groups of 6 animals in each treatment and control groups. The heifers of the treatment group were supplemented with bypass fat (100 g), bypass protein (950 g) and chelated mineral mixture (50 g) over the routine farm feeding practices of control group, and its effect on body weight gain and blood biochemical profiles were studied at a monthly interval during 6 months of experimental period. The overall effect of nutritional supplementation on animals body weight, blood glucose and total cholesterol over the control group was statistically non-significant (p greator than 0.05) yet beneficial. The plasma total protein levels in the supplemented group increased with a duration of supplement and levels were significantly (p less than 0.05) higher over the control group. The ovarian dynamics evaluated by transrectal ultrasound during last three months of the study revealed an increasing number of growing follicles greator than 4 mm in diameters (5.00 ± 0.13 to 6.67 ± 0.40), without significant variation in diameters of largest follicles (9.14 ± 0.45 to 10.27 ± 0.85 mm) between groups or periods. However, none of the heifers exhibited behavioral signs of estrus during 6 months study, in spite of the establishment of cyclicity with the presence of developed follicles, or CLs suggesting silent ovulation, at 36 ± 2 months of age in this breed.

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