scholarly journals ACTIVIDADE ANTIMICROBIANA E EFEITO COMBIANDO SOBRE DROGAS ANTIFÚNGICAS Y ANTIBACTERIANAS DO FRUTO DE Morinda citrifolia L.

2015 ◽  
Vol 20 (3) ◽  
pp. 193-200 ◽  
Author(s):  
Saulo Relison TINTINO ◽  
Abel Alves De Carvalho NETO ◽  
Irwin R. A. MENEZES ◽  
Cícera Datiane de M. OLIVEIRA ◽  
Henrique D. M. COUTINHO
Keyword(s):  
Escherichia Coli ◽  
Pseudomonas Aeruginosa ◽  
Fruit Tree ◽  
Morinda Citrifolia ◽  
Pharmacological Properties ◽  
Combined Effects ◽  
Drug Resistant ◽  
Fruit Extract ◽  
Antimicrobial Drugs ◽  
Para Determinar

<p>O aparecimento de micro-organismos resistentes e a toxicidade associada aos fármacos antimicrobianos aumentam a necessidade de pesquisas por novos princípios ativos. <em>Morinda citrifolia </em>L., uma planta frutífera utilizada popularmente como antibacteriano e com diversos outros usos farmacológicos. Possui diversos metabólitos primários e secundários, principalmente flavonóides, triterpenóides e alcalóides. O presente trabalho visou testar o potencial antimicrobiano e modulador dos extratos do seu fruto. Os extratos do fruto foram testados quanto ao seu efeito antimicrobiano e em combinação com antibacterianos e antifúngicos contra microrganismos patógenos pelo método de microdiluição em caldo. Observou-se que as associações entre antibióticos e extratos mostraram resultados com relevância clínica diante dos testes com bactérias <em>Pseudomonas aeruginosa</em> e <em>Escherichia coli</em>. </p><p><strong>Actividad antimicrobiana y efecto</strong> <strong>combinado sobre medicamentos antifúngicos y antibacterianos del fruto de <em>Morinda citrifolia</em> L.</strong></p><p>La aparición de microorganismos resistentes y la toxicidad asociada a los antimicrobianos aumenta la necesidad de investigar nuevos principios activos. <em>Morinda citrifolia</em> L., es una planta fructífera utilizada popularmente como antibacteriano y con otros diversos usos farmacológicos. Posee diversos  metabolitos primarios y secundarios, principalmente flavonoides, triterpenoides y alcaloides. Este estudio tuvo como objetivo ensayar la potencial actividad antimicrobiana y moduladora de los extractos obtenidos de su fruta. Los extractos de las frutas fueron testados tanto para determinar el efecto antimicrobiano como también utilizándolo en combinación con agentes antibacterianos y antifúngicos contra microorganismos patógenos, por el método de microdilución en caldo. Se observó que la asociación entre antibióticos y extractos dió resultados clínicamente significativos en pruebas con bacterias <em>Pseudomonas aeruginosa </em>y <em>Escherichia coli</em>. </p><p><strong>Antimicrobial Activity and Combined Effects on Antifungal and Antibacterial Drugs the Fruit of <em>Morinda citrifolia </em>L.</strong></p><p>The rising of the drug resistant microrganisms and the toxicity related with the antimicrobial drugs enhances the necessity about the search of new bioactive compounds. <em>Morinda citrifolia </em>L. is a fruit tree traditionally used as antibacterial and with several other pharmacological properties. This plant presents several phytocompounds as flavonoids, alkaloids and triterpenes. The objective of this work was assay the antimicrobial and modulatory potential of the fruit extract. The extract was assayed alone and associated with antibiotics and antifungals against pathogenic microrganisms using the microdilution assay. The results demonstrated that the association between extract and antimicrobial drugs resulted in a potentiation of the drug activity clinically relevant against <em>Pseudomonas aeruginosa</em> and <em>Escherichia coli</em>.</p>

scholarly journals The Study of Antibacterial Properties of Anbarnasara Smoke on Multi-Drug Resistant Bacteria Isolated From Urinary Infection in Pregnant Women

2020 ◽  
Vol 8 (4) ◽  
pp. 110-115
Author(s):  
Afsaneh Molamirzaei ◽  
Maryam Allahdadian ◽  
Monir Doudi
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Pregnant Women ◽  
Urinary Infection ◽  
Antibacterial Properties ◽  
Resistant Bacteria ◽  
Klebsiella Pneumonia ◽  
Drug Resistant

Background: Using smoke from burning donkey dung has been popular in the treatment of many diseases in Iran. Objective: This study aimed to investigating the antimicrobial properties of donkey dung smoke on multi-drug resistant (MDR) bacteria isolated from urinary infection. Materials and Methods: First, 300 and 200 urine samples were collected from pregnant and non-pregnant women in Isfahan, Iran. Then in each group, 100 bacterial isolates including Escherichia coli, Klebsiella pneumonia, Proteus vulgaris, Staphylococcus epidermidis, Staphylococcus aureus, Pseudomonas aeruginosa, and Staphylococcus saprophyticus were isolated. Antibiotic resistant protocol was determined by antibiogram test. Donkey dung was sterilized, disintegrated, and heated. The smokes were concentrated in n-hexane solvent (65%) and were collected after evaporation of the solvent. Finally, the antibacterial activities of the concentrations of 0.25, 0.5 and 1 mg/mL of the smokes were detected using disk diffusion and macrodilution methods. Results: The most abundant MDR isolates causing urinary infections in pregnant and non-pregnant women was Escherichia coli. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of donkey dung smoke on MDR isolates from pregnant women were 0.25 mg/mL and 0.5 mg/mL, respectively. In the case of MDR isolates in non-pregnant women, the MIC of the smoke on Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus aureus was 0.25 mg/mL, and the MBC on these isolates was 0.5 mg/mL. Conclusion: The smokes from donkey dung investigated in the present study have suitable potentials for controlling the infections after In vivo analysis.

scholarly journals Phytochemical Activities of Artemissia absinthium and Butea monosperma Plant Extracts

2021 ◽  
pp. 1-7
Author(s):  
Fatima Waheed ◽  
Shaista Yaqoob ◽  
Qurban Ali ◽  
Arif Malik
Keyword(s):  
Escherichia Coli ◽  
Pseudomonas Aeruginosa ◽  
Plant Extracts ◽  
Antimicrobial Activities ◽  
Diffusion Method ◽  
Antimicrobial Drugs ◽  
Butea Monosperma ◽  
Fungal Strains ◽  
Inhibition Zones ◽  
Agar Well Diffusion Method

With the developing time, there is a tremendous need to deliver highly potential antimicrobial drugs and treatment from different herbaceous plants because plants are now days commonly used to provide resistance against disease-causing pathogens. This study has been done by using agar well diffusion method. The bacterial along with fungal strains were collected and were cultured on agar plates. Afterwards, these plates were put in incubate for 24 hours at 370C temperature. Within this duration, the zones were developing all around the plates, wherever plant extracts were poured. The activities of microorganisms were measured by using inhibition diameter zones. The inhibition zones were shown clearly on petri plates. Antimicrobial activities of plant extracts of Artemissia absinthium and Butea monosperma tested against bacterial and fungal strains viz. Pseudomonas aeruginosa, Bacillus subtilis and Escherichia coli as well as fungal strains were also used viz. Aspergillus niger and Fusarium oxysporum.

scholarly journals Actividad antibacteriana de extractos hexánico y etanólico de macroalgas marinas de la Bahía de Ancón, Lima – Perú

2020 ◽  
Vol 31 (2) ◽  
pp. e17829
Author(s):  
Ramón Muñoz A. ◽  
Selma Santome ◽  
Jorge León Q.
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Bacillus Subtilis ◽  
Salmonella Typhimurium ◽  
San Francisco ◽  
Subtilis Atcc ◽  
Bacillus Subtilis Atcc ◽  
Para Determinar

En el presente trabajo se evaluaron las actividades antimicrobianas de extractos etanólicos y hexánicos de nueve especies de macroalgas marinas recolectadas en la zona intermareal de la playa San Francisco, Ancón, de Lima, Perú. La evaluación se realizó in vitro frente a un panel de bacterias estándar Gram positivas (Staphylococcus aureus ATCC 6538, Bacillus subtilis ATCC 6633 y Enterococcus faecalis ATCC 51922) y Gram negativas (Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 9027 y Salmonella Typhimurium ATCC 14028). Los extractos etanólicos fueron de mayor rendimiento (67%) en comparación a los hexánicos (33%), en especial frente a los Gram positivos, siendo el extracto obtenido de Ulva enteromorpha var. intestinalis y U. nematoidea los más activos frente a S. aureus ATCC 6538 (74.1 y 78.2 % de inhibición, respectivamente). Los extractos etanólicos de Cladophora sp y U. nematoidea frente a B. subtilis ATCC 6633 presentaron inhibiciones entre moderada y baja (52.5 y 33%, respectivamente). Los extractos etanólicos de U. enteromorpha y Cladophora sp frente a S. Typhimurium presentaron solo 40% de inhibición. El extracto etanólico de Cladophora sp fue el único que presentó actividad antimicrobiana frente a las cinco cepas bacterianas y fue elegida para determinar la Concentración Mínima Inhibitoria (CMI), que resultó en 12.5 y 25 mg/ml frente a B. subtilis ATCC 6633 y S. aureus ATCC 6538, respectivamente. Se concluye que, la mayoría de las macroalgas estudiadas contienen compuestos inhibitorios, cuyos extractos etanólico y hexánico son capaces de inhibir el crecimiento de bacterias patógenas.

scholarly journals Pola Sensitivitas Isolat Klinis Bakteri Gram Negatif Multiresisten Obat Terhadap Fosfomisin dari Beberapa Rumah Sakit Kota Pekanbaru

2019 ◽  
Vol 13 (1) ◽  
pp. 28
Author(s):  
Dewi Anggraini ◽  
Atya Nasmah ◽  
Fauzia Andrini
Keyword(s):  
Escherichia Coli ◽  
Pseudomonas Aeruginosa ◽  
Clinical Isolate ◽  
Alternative Therapy ◽  
Drug Resistant ◽  
Microbiology Laboratory ◽  
Cross Sectional ◽  
Gram Negative ◽  
Sensitivity Pattern

Over the last few years, there has been a decrease in amount of effective antibiotics, coincide with the lack of newantibiotics relevant for the treatment of Gram negative multi-drug resistant organisms infections. This problem can beovercome by the assessment and re-introduction of old antibiotics such as fosfomycin. Fosfomycin is a broad spectrumantibiotic that is bactericidal. This study aimed to find out the sensitivity pattern of clinical isolate multi-drug resistantGram negative bacteria to fosfomycin with descriptive cross sectional method. The sample used in this study wasclinical isolates collection of Microbiology Laboratory Faculty of Medicine, University of Riau from Hospitals inPekanbaru. The result showedsensitivity to fosfomycin Escherichia coli producing ESBL (88%), Klebsiella pneumoniaeproducing ESBL (95%), Acinotobacter baumannii resistant meropenem (27%), Pseudomonas aeruginosa resistantmeropenem (36%) and K.pneumoniae resistant meropenem (70%). Fosfomycin can be used as alternative therapy forinfections caused by Gram-negative Multi-drug resistant organisms.

Use of sterile maggots to treat panniculitis in an aged donkey

2001 ◽  
Vol 149 (25) ◽  
pp. 768-770 ◽  
Author(s):  
N. J. Bell ◽  
S. Thomas
Keyword(s):  
Escherichia Coli ◽  
Pseudomonas Aeruginosa ◽  
Surgical Treatment ◽  
Bacterial Culture ◽  
Skin Wound ◽  
Lucilia Sericata ◽  
Drug Resistant ◽  
Moderate Growth ◽  
The Common

An aged female donkey developed a severe, localised, suppurative panniculitis secondary to a skin wound. Bacterial culture of swabs taken from the wound gave a profuse growth of multi-drug-resistant Pseudomonas aeruginosa, a profuse growth of Escherichia coli and a moderate growth of beta-haemolytic Streptococcus species. The lesion did not respond to conventional medical and surgical treatment and continued to progress. Six applications of sterile larvae (maggots) of the common greenbottle, Lucilia sericata, were used to debride the wound successfully.

scholarly journals Synthesis and Study of New Quinolineaminoethanols as Anti-Bacterial Drugs

2019 ◽  
Vol 12 (2) ◽  
pp. 91
Author(s):  
Laumaillé ◽  
Dassonville-Klimpt ◽  
Peltier ◽  
Mullié ◽  
Andréjak ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Klebsiella Pneumoniae ◽  
Resistant Bacteria ◽  
Drug Resistant ◽  
E Coli ◽  
Gram Positive Bacteria ◽  
Drug Resistant Bacteria ◽  
Escherichia Coli Bacteria

The lack of antibiotics with a novel mode of action associated with the spread of drug resistant bacteria make the fight against infectious diseases particularly challenging. A quinoline core is found in several anti-infectious drugs, such as mefloquine and bedaquiline. Two main objectives were set in this work. Firstly, we evaluated the anti-mycobacterial properties of the previous quinolines 3, which have been identified as good candidates against ESKAPEE (Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, Enterobacter spp. and Escherichia coli) bacteria. Secondly, a new series 4 was designed and assessed against the same bacteria strains, taking the pair of enantiomers 3m/3n as the lead. More than twenty compounds 4 were prepared through a five-step asymmetric synthesis with good enantiomeric excesses (>90%). Interestingly, all compounds of series 3 were efficient on M. avium with MIC = 2–16 µg/mL, while series 4 was less active. Both series 3 and 4 were generally more active than mefloquine against the ESKAPEE bacteria. The quinolines 4 were either active against Gram-positive bacteria (MIC ≤ 4 µg/mL for 4c–4h and 4k/4l) or E. coli (MIC = 32–64 µg/mL for 4q–4v) according to the global lipophilicity of these compounds.

scholarly journals Evaluación de métodos para medir la actividad inhibitoria de extractos vegetales nativos del departamento de sucre sobre bacterias y levadura patógenas

2011 ◽  
Vol 3 (1) ◽  
pp. 90
Author(s):  
ALEXANDER PEREZ C ◽  
JOHANNA ROJAS S ◽  
JOHANA RODRIGUEZ ◽  
ARTURO DONCEL M ◽  
IRMA ARRIETA A ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Pseudomonas Aeruginosa ◽  
Enterococcus Faecalis ◽  
Klebsiella Oxytoca ◽  
Psidium Guajava ◽  
Melissa Officinalis ◽  
Cymbopogon Citratus ◽  
Origanum Vulgare ◽  
Para Determinar

El objetivo del presente trabajo fue evaluar la eficiencia de dos métodos para medir actividad inhibitoria de extractos vegetales etanólicos nativos del Departamento de Sucre sobre bacterias y levadura patógenas de humanos y plantas. Los muestreos se realizaron en los municipios de Morroa y Sincelejo. Las hojas de las plantas Melia Azederach, Sapium sp, Jatropha gossypilia, Psidium guajava, Origanum vulgare Melissa officinalis, Eucalyptus sp y Cymbopogon citratus fueron procesadas utilizando los métodos de percolación y de soxhlet para la extracción de los extractos. Se evaluaron los métodos de orificio sobre agar (método 1) y el método de disco sensitivos (método 2) para medir el efecto inhibitorio sobre Pseudomonas aeruginosa, Klebsiella oxytoca, Enterococcus faecalis, Escherichia coli, Bulkolderia glumaea y Zygosaccharomyces microellipsoides. Los resultados obtenidos demuestran que el método 2 permite una mayor eficacia e interpretación de los resultados con relación al método 1 donde se observó difusión de los extractos en el orificio, lo cual crea interferencia en la medición. Este es el primer trabajo en el departamento de Sucre donde se evalúan dos métodos para determinar actividad inhibitoria de un grupo amplio de plantas nativas sobre bacterias y levadura patógenas.

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