scholarly journals NUTRIENT RECOVERY AND POLLUTANT REMOVAL FROM PIGGERY WASTEWATER BY SPIRULINA CULTIVATION

2020 ◽  
Vol 58 (3A) ◽  
pp. 187
Author(s):  
Yen Thi Thai Doan ◽  
Nga Thuy Nguyen ◽  
My Tra Phung ◽  
Chau Ngoc Nguyen ◽  
Huong Thi Do

Spirulina is noticed for great applications on food supplements, animal feeds, pharmaceutical, cosmetics, biofuel, fertilizer, etc. Spirulina cultures in wastewaters could enhance the feasibility of commodities due to its ability to reduce the cost of biomass production and remove pollutants in wastewaters. This study investigates the effects of wastewater pretreatment using various aeration periods (i.e. from 3 to 7 days), the supplement of bicarbonate and N:P ratios on the growth of Spirulina sp. HH to produce protein-rich biomass. The work showed that Spirulina sp. had the ability to effectively remove ammonium, with the highest efficiencies up to 99.9 %. However, the high concentration of ammonium in wastewater, from 125 mg N/L upward, caused a decline in the growth rate of Spirulina. The growth and remediation potential of Spirulina sp. were in the best condition with the N:P ratio of the medium in the range of 19:1-22:1. This study suggested a procedure to cultivate Spirulina sp. in piggery wastewater and remove pollutants efficiently.

Membranes ◽  
2021 ◽  
Vol 11 (11) ◽  
pp. 874
Author(s):  
Yi Ding ◽  
Shiyuan Wang ◽  
Hang Ma ◽  
Binyu Ma ◽  
Zhansheng Guo ◽  
...  

The nutrient removal and biomass production of the internal circulating fluidized bed microalgae membrane bioreactor (ICFB-MMBR) was studied under different cultivation modes, influent TOC, influent pH, and influent N/P. Platymonas helgolandica tsingtaoensis was used as the biological source. The growth of P. helgolandica tsingtaoensis and the removal efficiency of pollutants in the mixotrophy culture mode were improved compared with other culture modes. With the increased influent TOC, the average growth rate of P. helgolandica tsingtaoensis increased, and ammonia nitrogen and total phosphorus removal rate were improved. The P. helgolandica tsingtaoensis growth rate and nutrient removal efficiencies at the influent pH of 8 were the best among the different influent pH values. As the influent N/P ratio increased from 5 to 20, the P. helgolandica tsingtaoensis growth rate and pollutant removal rate increased gradually. When the influent N/P ratio was higher than 20, the P. helgolandica tsingtaoensis growth rate and pollutant removal rate tended to be stable and did not significantly change with the increase of influent N/P ratio. At the proper influent conditions, the high P. helgolandica tsingtaoensis biomass and nutrient removal efficiency could be obtained in the microalgae membrane bioreactor, which could provide a theoretical basis for the application of the system for wastewater treatment.


2018 ◽  
Vol 1 (1) ◽  
pp. 15-22 ◽  
Author(s):  
Vivian Ayu Cyntya ◽  
Gunawan Widi Santosa ◽  
Endang Supriyantini ◽  
Sri Yulina Wulandari

Rumput laut merupakan tumbuhan yang tidak dapat dibedakan antara akar, batang dan daun, sehingga seluruh bagian tubuhnya disebut dengan thallus. Rumput laut Gracilaria sp. merupakan salah satu sumber daya laut yang mudah dibudidayakan, mempunyai nilai ekomonis penting dan mempunyai prospek pasar yang cerah, baik di dalam negeri maupun luar negeri. Usaha budidaya rumput laut Gracilaria sp. perlu dilakukan guna meningkatkan produksinya. Rumput laut Gracilaria sp. memerlukan N dan P sebagai unsur hara makro yang digunakan untuk pertumbuhan. Penelitian ini bertujuan untuk mengetahui pengaruh penambahan rasio N:P yang berbeda terhadap laju pertumbuhan Gracilaria sp. Metode yang digunakan adalah metode eksperimental dengan Rancangan Acak Lengkap (RAL). Penelitian ini menggunakan 3 perlakuan dan 1 kontrol, yang masing-masing ada 3 ulangan. Media pemeliharaan menggunakan air laut yang ditambah N:P dengan rasio yang berbeda, yaitu : A (Kontrol), B (6:1), C (6:2), D (6:3). Pencapaian berat rerata Gracilaria sp. selama 28 hari adalah sebagai berikut : A = 102,06 ± 0,04 g; B = 103,41 ± 0,57 g; C = 106,53 ± 0,66 g dan D = 108,28 ± 1,25 g. Pertumbuhan mutlak Gracilaria sp. yang dihasilkan selama penelitian adalah sebagai berikut : A = 2,06 ± 0,04 g;B = 3,41 ± 0,57 g; C = 6,53 ± 0,66 g; D = 8,28 ± 1,25 g. Laju pertumbuhan spesifik Gracilaria sp. yang dihasilkan selama penelitian, yaitu : A sebesar 0,07 ± 0,00 % berat (g) per hari; B sebesar 0,12 ± 0,02 % berat (g) per hari; C sebesar 0,23 ± 0,02 % berat (g) per hari dan D sebesar 0,28 ± 0,04 % berat (g) per hari. Hasil penelitian menunjukkan bahwa penambahan N:P dengan rasio yang berbeda berpengaruh nyata terhadap pertumbuhan mutlak dan laju pertumbuhan spesifik Gracilaria sp. (p < 0,01). Semakin tinggi konsentrasi rasio N:P dapat meningkatkan total biomassa dan laju pertumbuhan spesifik.   Seaweed is a plant which can not be distinguished between true roots, stalks and leaves, therefore its entire body is called a thallus. Gracilaria sp. is a seaweed which is relatively easy to cultivate, has high economical value and bright marketing prospect either in domestic or foreign markets. The effort to cultivate Gracilaria sp. needs to be done in order to increase the production. Seaweed Gracilaria sp. needs N and P as important macronutrients for its growth. This research aims to discover the effect of adding different N:P ratio towards Gracilaria sp. growth rate. The method used in this research was an experimental method with Completely Randomized Design. This research used three treatments and one control, each with three repetitions. The cultivation media was seawater with the addition of different N:P ratios, which were: A (Control), B (6:1), C (6:2) and D (6:3). The average weight of Gracilaria sp. for 28 days were as follows: A = 102,06 ± 0,04 g; B = 103,41 ± 0,57 g; C = 106,53 ± 0,66 g and D = 108,28 ± 1,25 g. The absolute growth of Gracilaria sp. during this research were: A = 2,06 ± 0,04 g;B = 3,41 ± 0,57 g; C = 6,53 ± 0,66 g; D = 8,28 ± 1,25 g. The specific growth rate for Gracilaria sp. during this research were: A = 0,07 ± 0,00 % weight (g) per day; B = 0,12 ± 0,02 % weight (g) per day; C = 0,23 ± 0,02 % weight (g) per day and D = 0,28 ± 0,04 % weight (g) per day. The result of this research showed that the addition of different N:P ratios caused a significant effect on the absolute growth and the spesific growth rate of Gracilaria sp. (p < 0,01). The higher concentratrion ratio of N:P given the higher either total biomassa and specific growth rate achieved.


2013 ◽  
Vol 85 (4) ◽  
pp. 1427-1438 ◽  
Author(s):  
MATHIAS A. CHIA ◽  
ANA T. LOMBARDI ◽  
MARIA DA GRACA G. MELAO

The need for clean and low-cost algae production demands for investigations on algal physiological response under different growth conditions. In this research, we investigated the growth, biomass production and biochemical composition of Chlorella vulgaris using semi-continuous cultures employing three growth media (LC Oligo, Chu 10 and WC media). The highest cell density was obtained in LC Oligo, while the lowest in Chu medium. Chlorophyll a, carbohydrate and protein concentrations and yield were highest in Chu and LC Oligo media. Lipid class analysis showed that hydrocarbons (HC), sterol esthers (SE), free fatty acids (FFA), aliphatic alcohols (ALC), acetone mobile polar lipids (AMPL) and phospholipids (PL) concentrations and yields were highest in the Chu medium. Triglyceride (TAG) and sterol (ST) concentrations were highest in the LC Oligo medium. The results suggested that for cost effective cultivation, LC Oligo medium is the best choice among those studied, as it saved the cost of buying vitamins and EDTA associated with the other growth media, while at the same time resulted in the best growth performance and biomass production.


2004 ◽  
Vol 31 (12) ◽  
pp. 1149 ◽  
Author(s):  
István Molnár ◽  
László Gáspár ◽  
Éva Sárvári ◽  
Sándor Dulai ◽  
Borbála Hoffmann ◽  
...  

The physiological and morphological responses to water stress induced by polyethylene glycol (PEG) or by withholding water were investigated in Aegilops biuncialis Vis. genotypes differing in the annual rainfall of their habitat (1050, 550 and 225 mm year–1) and in Triticum aestivum L. wheat genotypes differing in drought tolerance. A decrease in the osmotic pressure of the nutrient solution from –0.027 to –1.8 MPa resulted in significant water loss, a low degree of stomatal closure and a decrease in the intercellular CO2 concentration (Ci) in Aegilops genotypes originating from dry habitats, while in wheat genotypes high osmotic stress increased stomatal closure, resulting in a low level of water loss and high Ci. Nevertheless, under saturating light at normal atmospheric CO2 levels, the rate of CO2 assimilation was higher for the Aegilops accessions, under high osmotic stress, than for the wheat genotypes. Moreover, in the wheat genotypes CO2 assimilation exhibited less or no O2 sensitivity. These physiological responses were manifested in changes in the growth rate and biomass production, since Aegilops (Ae550, Ae225) genotypes retained a higher growth rate (especially in the roots), biomass production and yield formation after drought stress than wheat. These results indicate that Aegilops genotypes, originating from a dry habitat have better drought tolerance than wheat, making them good candidates for improving the drought tolerance of wheat through intergeneric crossing.


1998 ◽  
Vol 42 (8) ◽  
pp. 1900-1905 ◽  
Author(s):  
George S. Baillie ◽  
L. Julia Douglas

ABSTRACT A perfused biofilm fermentor, which allows growth-rate control of adherent microbial populations, was used to assess whether the susceptibility of Candida albicans biofilms to antifungal agents is dependent on growth rate. Biofilms were generated under conditions of glucose limitation and were perfused with drugs at a high concentration (20 times the MIC). Amphotericin B produced a greater reduction in the number of daughter cells in biofilm eluates than ketoconazole, fluconazole, or flucytosine. Similar decreases in daughter cell counts were observed when biofilms growing at three different rates were perfused with amphotericin B. In a separate series of experiments, intact biofilms, resuspended biofilm cells, and newly formed daughter cells were removed from the fermentor and were exposed to a lower concentration of amphotericin B for 1 h. The susceptibility profiles over a range of growth rates were then compared with those obtained for planktonic cells grown at the same rates under glucose limitation in a chemostat. Intact biofilms were resistant to amphotericin B at all growth rates tested, whereas planktonic cells were resistant only at low growth rates (≤0.13 h−1). Cells resuspended from biofilms were less resistant than intact biofilm populations but more resistant than daughter cells; the susceptibilities of both these cell types were largely independent of growth rate. Our findings indicate that the amphotericin B resistance of C. albicans biofilms is not simply due to a low growth rate but depends on some other feature of the biofilm mode of growth.


Author(s):  
Kaustav Mukherjee

Abstract: The present study entitled “Development of protein rich flavoured bar” was conducted with the objective to develop protein rich flavoured bar using different ingredients, to assess the sensory accessibility, determine the nutritional composition and cost of developed protein bar. Protein rich flavoured bar were prepared by using three treatments i.e. T1 (dates 50g, oats 10g, flaxseeds 5g, sesame seeds 5g, pumpkin seeds 5g, peanut powder 10g, honey 5g, cocoa powder 10g), T2 (dates 45g, oats 8g, flaxseeds 5g, sesame seeds 5g, pumpkin seeds 5g, peanut powder 10g, honey 12g, guava flavour 10g) and T3 (dates 40g, oats 13g, flaxseeds 5g, sesame seeds 5g, pumpkin seeds 5g, peanut powder 10g, honey 12g, orange flavour 10g). Organoleptic evaluation of the prepared product in relation to sensory attributes was carried out using the nine point hedonic scale score card by Srilaksmi (2015). The nutrient content of the value added food products were calculated with the help of food composition table given by Gopalan et al., (2011). The cost of individual raw ingredients used in the preparation of the food product as the prevailing market price. All treatments were replicated four times and the data obtained during investigation were statistically analyzed by using analysis of variance (ANOVA) and critical difference (C.D.) techniques. On the basis of sensory acceptability it was found that T1 was scored highest in terms of colour and appearance, body and texture, flavour and taste and overall acceptability. As well as T1 shows significantly high in the nutritive value among all treatments regarding energy, protein, carbohydrates, fat, fibre, calcium and iron. The cost of the protein rich flavoured bar per 100g of dry ingredients at the prevailing cost of the raw materials was highest in T1 (Rs. 29.33) followed by T2 (Rs. 20.69) and T3 (Rs. 20.34). Dates are very good source of fibre, carbohydrate, protein and act as natural sweetener with no fat. As the bar is rich in protein, iron and other macronutrients, so it is majorly recommended for Protein-energy malnutrition (PEM), athletes and anemic patient. Daily 100g of dates intake helps to get all essential nutrients. Strictly restricted for Type-1 diabetic patients. Keywords: Protein, Nutrient content, organoleptic evaluation, nutrition bar, cost.


2020 ◽  
Vol 4 (2) ◽  
pp. 577-583
Author(s):  
M. B. Usman ◽  
O. S. Aaasa ◽  
O. S. Balogun ◽  
U. F. Yahaya

This study investigated the marketing of frozen fish in Kaduna metropolis, Kaduna state. Ten (10) markets were purposively selected due to high concentration of frozen fish marketers and the volume of trading activities; Primary data were generated through the use of structured questionnaire administered to hundred (100) randomly selected retailers and fifteen (15) purposively selected wholesalers. The data were analyzed using descriptive statistic, budgeting technique, net marketing and rate of return on capital invested. The result show that majority 80 percent and 53 of the retailers and wholesalers were female, Majority (70prcent) of the retailers had primary education but most of the wholesaler’s attained tertiary education. The frozen fish marketing channels identified in the area are made up of zero and multi stage channels. Furthermore, the cost and return analysis revealed that the wholesalers realized about N121, 000.00 naira while the retailers got N56, 000.00 naira per month while return to per capital invested (RPCI) was 11 kobo and 22 kobo per Naira invested for the wholesalers and the retailers respectively. This implies that the enterprise is profitable. Transportation difficulties and marketing charges ranked were major constraints confronting frozen fish marketers. It is recommended that provision of good roads network and formidable integrated marketing system will further improve the profitability of the enterprise in the study area.


2012 ◽  
Vol 30 (1) ◽  
pp. 103-122
Author(s):  
Richard J. Cebula ◽  
Maggie Foley

Abstract This study empirically investigates three hypotheses. The first is that higher levels of economic freedom in an economy promote a higher growth rate of economic activity and hence yield a higher growth rate of per capita real GDP in that economy. The second hypothesis is that higher quality government regulation leads to a more efficient economic system, in large part by interfering less with market functioning and in part by not adding unnecessarily to the cost of conducting business in the marketplace, and thereby leads to a higher per capita real GDP growth rate. The third hypothesis is that the higher the taxation level/burden relative to GDP in an economy, the lower the growth rate of private sector spending and hence the lower the growth rate of per capita real GDP in that economy. Using a panel dataset for OECD nations over the 2003 through 2006 period, fixed effects PLS estimations find compelling evidence in support of all three of these hypotheses.


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