scholarly journals Influence of water pH in oral changes caused by cadmium poisoning: an experimental study in rats

2014 ◽  
Vol 43 (3) ◽  
pp. 180-184 ◽  
Author(s):  
Bianca Keller Lima MARTELLI ◽  
Diogo Milani de MELO ◽  
Gisele Alborghetti NAI ◽  
José Luiz Santos PARIZI
Keyword(s):  
Drinking Water ◽  
Salivary Glands ◽  
Buccal Mucosa ◽  
Chloride Solution ◽  
Cadmium Chloride ◽  
Microscopic Analysis ◽  
Acidic Ph ◽  
High Concentration ◽  
Neutral Ph ◽  
Cadmium Poisoning

Objective: This study aims to evaluate the effect of the pH of drinking water in the oral changes caused by cadmium poisoning. Material and method: Ninety male Wister rats were divided into the following six groups: A - 15 rats were given cadmium chloride solution (400 mg/L) in drinking water with a neutral pH (pH 7.0); B - 15 rats received cadmium chloride solution (400 mg/L) in drinking water with an acidic pH (pH 5.0); C - 15 rats were treated with a cadmium chloride solution (400 mg/L) in drinking water with a basic pH (pH 8.0); D - 15 rats received drinking water with an acidic pH (pH 5.0); E - 15 rats were given drinking water with a basic pH (pH 8.0); F - 15 rats received water with a neutral pH (pH 7.0). All animals were sacrificed six months after the beginning of the experiment. A biopsy of the buccal mucosa, tongue and salivary gland of each animal was taken for microscopic analysis. Result: No changes were observed in the buccal mucosa, tongue mucosa or salivary glands in any of the groups. Conclusion: Drinking water that contains a high concentration of cadmium with differing pH levels demonstrated no damage to the oral mucosa and salivary glands of male Wistar rats.

scholarly journals Influence of water pH in the hepato- and nephrotoxicity of chronic cadmium poisoning in Wistar rats

2021 ◽  
Vol 10 (9) ◽  
pp. e12210917753
Author(s):  
Daniel José Pimentel Bonfim ◽  
Fernanda Maria Garcia ◽  
Cecília Braga Laposy ◽  
Rogério Giuffrida ◽  
Gisele Alborghetti Nai ◽  
...  
Keyword(s):  
Heavy Metal ◽  
Drinking Water ◽  
Cadmium Chloride ◽  
Albino Rats ◽  
Acidic Ph ◽  
Alkaline Ph ◽  
Hydropic Degeneration ◽  
Neutral Ph ◽  
Cadmium Poisoning ◽  
Water Ph

Introduction: Cadmium is a heavy metal found in the environment that is used industrially; however, it also causes hepato- and nephrotoxic effects. Objective: To evaluate the effect of drinking water pH on the hepato- and nephrotoxicity caused by chronic cadmium poisoning. Material and Methods: We used 90 adult, male Wistar albino rats divided into 6 groups (n = 15): GC5 received a solution of cadmium chloride in drinking water with an acidic pH (pH 5.0); GC7 received a solution of cadmium chloride (400 mg/L) in drinking water with a neutral pH (pH 7.0 water); GC8 received a solution of cadmium chloride in water with an alkaline pH (pH 8.0); GWC5 received drinking water with an acidic pH (pH 5.0); GWC7 received drinking water with a neutral pH (pH 7.0); GWC8 received drinking water with an alkaline pH (pH 8.0). The animals were euthanized 6 months after the start of the experiment. We performed tests for hepatic and renal function and conducted liver and renal histopathology. Results: Water with an acidic pH caused alterations in ALP, ALT and urea in animals exposed to cadmium (P<0.05). In the liver, the majority of animals from the GC7 (57.1%) and GC5 (53.3%) groups showed diffuse microvesicular steatosis, while other groups showed no steatosis (P>0.05). In the kidney, the majority of animals from the GC7 (78.6%) and GWC5 (71.4%) groups showed tubular hydropic degeneration; however, these data were only statistically different from the GWC7 group (P<0.05). Conclusion: Exposure to cadmium in water with an acidic pH led to higher elevations of serum ALP, AST and urea, suggesting that the pH of drinking water influences the hepato- and nephrotoxic effects of this heavy metal.

scholarly journals Proteolytic Processing of Pro-opiomelanocortin Occurs in Acidifying Secretory Granules of AtT-20 Cells

1997 ◽  
Vol 45 (3) ◽  
pp. 425-436 ◽  
Author(s):  
Shigeyasu Tanaka ◽  
Takao Yora ◽  
Kazuhisa Nakayama ◽  
Kinji Inoue ◽  
Kazumasa Kurosumi
Keyword(s):  
Secretory Granules ◽  
Specific Inhibitor ◽  
Tumor Cell Line ◽  
Microscopic Analysis ◽  
Proteolytic Processing ◽  
Acidic Ph ◽  
Electron Microscopic ◽  
Constitutive Secretion ◽  
Golgi Network ◽  
Acidic Organelles

Using antibodies specific for pro-opiomelanocortin (POMC), amidated joining peptide (JP), and the prohormone convertase PC1, we showed immunocytochemically that PC1 in a corticotrophic tumor cell line, AtT-20, was co-localized either with POMC or with amidated JP in secretory granules, and also confirmed that POMC was cleaved mainly in secretory granules. Analysis using DAMP (3- [2,4-dinitroanilino]-3'-amino- N-methyldipropylamine) as the pH probe suggested a correlation between POMC processing and acidic pH in the secretory granules. Bafilomycin A1, a specific inhibitor of vacuolar-type H+-AT-Pase, completely inhibited POMC processing and caused constitutive secretion of the unprocessed precursor. By contrast, chloroquine, a weak base that is known to neutralize acidic organelles, was unable to inhibit POMC processing. Electron microscopic analysis revealed that, in AtT-20 cells treated with bafilomycin A1, the trans-Golgi cisternae were dilated and few secretory granules were present in the cytoplasm. These observations suggest that acidic pH provides a favorable environment for proteolytic processing of POMC by PC1 but is not required, and that integrity of the trans-Golgi network and sorting of POMC into secretory granules are important for POMC processing. (J Histochem Cytochem 45:425–436, 1997)

scholarly journals The Listeria monocytogenes hemolysin has an acidic pH optimum to compartmentalize activity and prevent damage to infected host cells

2002 ◽  
Vol 156 (6) ◽  
pp. 1029-1038 ◽  
Author(s):  
Ian J. Glomski ◽  
Margaret M. Gedde ◽  
Albert W. Tsang ◽  
Joel A. Swanson ◽  
Daniel A. Portnoy
Keyword(s):  
Listeria Monocytogenes ◽  
Bacterial Pathogen ◽  
Cytolytic Activity ◽  
Host Cells ◽  
Adaptive Mutation ◽  
Acidic Ph ◽  
Listeriolysin O ◽  
Lysosomal Hydrolases ◽  
Ph Optimum ◽  
Neutral Ph

Listeria monocytogenes is a facultative intracellular bacterial pathogen that escapes from a phagosome and grows in the host cell cytosol. The pore-forming cholesterol-dependent cytolysin, listeriolysin O (LLO), mediates bacterial escape from vesicles and is ∼10-fold more active at an acidic than neutral pH. By swapping dissimilar residues from a pH-insensitive orthologue, perfringolysin O (PFO), we identified leucine 461 as unique to pathogenic Listeria and responsible for the acidic pH optimum of LLO. Conversion of leucine 461 to the threonine present in PFO increased the hemolytic activity of LLO almost 10-fold at a neutral pH. L. monocytogenes synthesizing LLO L461T, expressed from its endogenous site on the bacterial chromosome, resulted in a 100-fold virulence defect in the mouse listeriosis model. These bacteria escaped from acidic phagosomes and initially grew normally in cells and spread cell to cell, but prematurely permeabilized the host membrane and killed the cell. These data show that the acidic pH optimum of LLO results from an adaptive mutation that acts to limit cytolytic activity to acidic vesicles and prevent damage in the host cytosol, a strategy also used by host cells to compartmentalize lysosomal hydrolases.

ANALYSIS OF MT2A AND MT3 GENE EXPRESSION IN RAT'S LIVER AND KIDNEY IN RESPONSE TO CADMIUM CHLORIDE POISONING

2021 ◽  
pp. 38-42
Author(s):  
M. M. Ziatdinova ◽  
T. G. Yakupova ◽  
Ya. V. Valova ◽  
G. F. Mukhammadieva ◽  
D. O. Karimov ◽  
...  
Keyword(s):  
Gene Expression ◽  
Rat Liver ◽  
Cadmium Chloride ◽  
Transcriptional Activity ◽  
Female Rats ◽  
Expression Level ◽  
Cadmium Poisoning ◽  
Liver And Kidney ◽  
Dose Dependent ◽  
Higher Doses

The aim of this study was to investigate the expression of metallothionein genes in the liver and kidneys of rats with acute cadmium poisoning.Simulation of poisoning with cadmium chloride was carried out on white outbred female rats, divided into 4 groups depending on the dose of the injected toxicant. RNA samples isolated from rat liver and kidneys were used as research materials.The multiplicity of expression of the MT3 gene in the kidneys increased at the lowest dose of CdCl2 , which was used in this experiment (0.029 mg / kg); with increasing dosage, the expression level decreased, but not lower than the control values. Analysis of the expression of the same gene in the liver showed a tendency towards a decrease in the content of transcripts with increasing dose. The frequency of expression of the MT2A gene at higher doses of CdCl2 increased both in the liver and in the kidneys.In the present work, statistically significant dose-dependent changes in the expression multiplicity of metallothionein genes were detected 24 hours after CdCl2 administration. The revealed differences in the level of transcriptional activity of metallothionein genes require further investigation, since there are probably differences in the level of gene expression at earlier and later periods of toxicant action.

NMR studies of exocyclic 1,N2-propanodeoxyguanosine adducts (X) opposite purines in DNA duplexes: protonated X(syn).cntdot.A(anti) pairing (acidic pH) and X(syn).cntdot.G(anti) pairing (neutral pH) at the lesion site

Biochemistry ◽  
1989 ◽  
Vol 28 (13) ◽  
pp. 5647-5657 ◽  
Author(s):  
Michael Kouchakdjian ◽  
Edmund Marinelli ◽  
Xiaolian Gao ◽  
Francis Johnson ◽  
Arthur Grollman ◽  
...  
Keyword(s):  
Acidic Ph ◽  
Dna Duplexes ◽  
Lesion Site ◽  
Nmr Studies ◽  
Neutral Ph

scholarly journals Calcium fluxes in Hoplosternum littorale (tamoatá) exposed to different types of Amazonian waters

2009 ◽  
Vol 7 (3) ◽  
pp. 465-470 ◽  
Author(s):  
Bernardo Baldisserotto ◽  
Carlos Eduardo Copatti ◽  
Levy Carvalho Gomes ◽  
Edsandra Campos Chagas ◽  
Richard Philip Brinn ◽  
...  
Keyword(s):  
Organic Carbon ◽  
Water Transfer ◽  
Well Water ◽  
Acidic Ph ◽  
Clear Water ◽  
Black Water ◽  
Neutral Ph ◽  
Different Types ◽  
White Water ◽  
Hoplosternum Littorale

Fishes that live in the Amazonian environment may be exposed to several kinds of waters: "black waters", containing high dissolved organic carbon and acidic pH, "white waters", with ten fold higher Ca2+ concentrations than black waters and neutral pH, and "clear waters", with two fold higher Ca2+ concentrations than black waters and also neutral pH. Therefore, the aim of the present study was to analyze Ca2+ fluxes in the facultative air-breather Hoplosternum littorale (tamoatá) exposed to different Amazonian waters. Fishes were acclimated in well water (similar to clear water) and later placed in individual chambers for Ca2+ fluxes measurements. After 4 h, water from the chambers was replaced by a different type of water. Transfer of tamoatás to ion-poor black or acidic black water resulted in net Ca2+ loss only in the first 2 h of experiment. However, transfer from black or acidic black water to white water led to only net Ca2+ influxes. The results obtained allowed us to conclude that transfer of tamoatás to ion-poor waters (black and acidic black water) led to transient net Ca2+ loss, while the amount of Ca2+ in the ion-rich white water seems adequate to prevent Ca2+ loss after transfer. Therefore, transfer of tamoatás between these Amazonian waters does not seem to result in serious Ca2+ disturbance.

scholarly journals ASSESSMENT OF GROUNDWATER QUALITY FROM INDUSTRIAL AND RESIDENTIAL AREAS OF SANGO OTA, NIGERIA

2021 ◽  
Vol 46 (2) ◽  
Author(s):  
O.A. Oguntade ◽  
V.I. I Fesiokwu ◽  
O. S. Sule
Keyword(s):  
Electrical Conductivity ◽  
Drinking Water ◽  
Water Samples ◽  
Total Suspended Solid ◽  
Industrial Area ◽  
Suspended Solid ◽  
Residential Area ◽  
Total Hardness ◽  
Residential Areas ◽  
High Concentration

High concentration of contaminants in drinking water can affect human health. This study assessed quality of groundwater at industrial and residential areas of Sango Ota, Ogun State. Water samples were collected in triplicates from 8 wells at industrial and residential areas and analyzed for its physicochemical properties. The pH, electrical conductivity, total dissolved solid (TDS), nitrate, sulphate, total suspended solid (TSS), total hardness (TH), iron, zinc, copper, lead, nickel, chromium and cadmium concentrations in the water samples were determined following American Public Health Association (APHA) procedure. Results showed that pH of 50 % of the wells were lower than minimum limit of 6.5 recommended by WHO. Sulphate, TH and iron in the water were significantly (p < 0.05) higher at residential area than industrial area. Electrical conductivity exhibited significant (p < 0.01) correlation with TDS (r = 0.701**) and NO32- (r = 0.922**) at residential area. At the industrial area however, concentration of salts in water samples was highly associated with SO42- (r = 0.864**) and Cd (r = 0.587**). Across locations of groundwater, iron and lead were above allowable WHO limits in drinking water. Cadmium was also above drinking limit at location T4 of the residential area. Consumers of groundwater in the study area are prone to health related challenges of heavy metal toxicity.

scholarly journals Metal Contamination in Ground Water of Dang District

1970 ◽  
Vol 9 ◽  
pp. 143-148 ◽  
Author(s):  
Rajendra K. Mahat ◽  
Rashmi Shrestha
Keyword(s):  
Water Quality ◽  
Drinking Water ◽  
Trace Metals ◽  
Ground Water ◽  
Atomic Absorption ◽  
Metal Contamination ◽  
Hydride Generation ◽  
Atomic Absorption Spectrophotometry ◽  
Drinking Water Quality ◽  
High Concentration

Drinking water quality in Nepal has been an issue of prevalent concern. So, this study was conducted to visualize the scenario of metal contamination in ground water of Dang district located at central west Terai in Nepal. A total of 523 water samples from tubewells and dugwells positioned in 16 village development committees (VDCs) were tested for arsenic in laboratory using atomic absorption spectrophotometer (AAS) employing continuous flow hydride generation technique. Randomly selected 20 samples were also tested for other heavy and trace metals like Mn, Fe, Cu, and Cd using AAS employing flame method. Of the total samples, 50.3% was found to contain arsenic above WHO drinking water qualty guidelines value of 10 ppb (0.01 mg/l) and 10.7% was found to contain arsenic above national drinking water quality guidelines value of 50 ppb (0.05 mg/l). The safest VDC is Sonpur while the most severely affected VDC is Gobardiha. A highest concentration of As of 240 ppb (0.24 mg/ l) was found in Dhikpur VDC. Ground water in this area seemed to be affected by high concentration of iron up to 11.01 mg/l and of manganese up to 0.51 mg/L. Statistical tools were employed to assess the probable association among metals but no significant correlation could be retrieved. Key words: hydride generation-atomic absorption spectrophotometry; redox conversion; arsenicosis; heavy and trace metals DOI: 10.3126/njst.v9i0.3178 Nepal Journal of Science and Technology 9 (2008) 143-148

scholarly journals Inactivation of tyrosine aminotransferase in neutral homogenates and rat liver slices

1972 ◽  
Vol 129 (5) ◽  
pp. 1131-1138 ◽  
Author(s):  
F. Auricchio ◽  
L. Mollica ◽  
A. Liguori
Keyword(s):  
Amino Acid ◽  
Rat Liver ◽  
Acid Concentration ◽  
Tyrosine Aminotransferase ◽  
Amino Acid Concentration ◽  
Acidic Ph ◽  
Ph Values ◽  
Neutral Ph ◽  
Liver Slices

Inactivation of tyrosine aminotransferase induced in vivo by triamcinolone was studied in a homogenate incubated at neutral pH values. The integrity and the presence of subcellular particles together with a compartment of acidic pH are necessary for inactivation of tyrosine aminotransferase. It is suggested that tyrosine aminotransferase is inactivated inside lysosomes. The system responsible for inactivation of tyrosine aminotransferase was partially purified and identified with lysosomal cathepsins B and B1. Inactivation of tyrosine aminotransferase in liver slices is controlled by the amino acid concentration and strongly stimulated by cysteine. 3,3′,5-Tri-iodo-l-thyronine reversibly and strongly decreases the rate of inactivation of tyrosine aminotransferase. The effect is not due to an increased rate of tyrosine aminotransferase synthesis.

scholarly journals Seasonal assessment, treatment and removal of heavy metal concentrations in a tropical drinking water reservoir

2016 ◽  
Vol 35 (2) ◽  
pp. 103-113 ◽  
Author(s):  
Moshood Keke Mustapha ◽  
Joy Chinenye Ewulum
Keyword(s):  
Heavy Metals ◽  
Drinking Water ◽  
Best Management Practices ◽  
Management Practices ◽  
Anthropogenic Activities ◽  
Health Hazard ◽  
Water Reservoir ◽  
Industrial Effluents ◽  
High Concentration ◽  
Agricultural Materials

AbstractHeavy metals are present in low concentrations in reservoirs, but seasonal anthropogenic activities usually elevate the concentrations to a level that could become a health hazard. The dry season concentrations of cadmium, copper, iron, lead, mercury, nickel and zinc were assessed from three sites for 12 weeks in Oyun reservoir, Offa, Nigeria. Triplicate surface water samples were collected and analysed using atomic absorption spectrophotometry. The trend in the level of concentrations in the three sites is site C > B > A, while the trend in the levels of the concentrations in the reservoir is Ni > Fe > Zn > Pb > Cd > Cu > Hg. Ni, Cd, Pb and Hg were found to be higher than the WHO guidelines for the metals in drinking water. The high concentration of these metals was from anthropogenic watershed run-off of industrial effluents, domestic sewages and agricultural materials into the reservoir coming from several human activities such as washing, bathing, fish smoking, especially in site C. The health effects of high concentration of these metals in the reservoir were highlighted. Methods for the treatment and removal of the heavy metals from the reservoir during water purification such as active carbon adsorption, coagulation-flocculation, oxidation-filtration, softening treatment and reverse osmosis process were highlighted. Other methods that could be used include phytoremediation, rhizofiltration, bisorption and bioremediation. Watershed best management practices (BMP) remains the best solution to reduce the intrusion of the heavy metals from the watershed into the reservoir.

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