scholarly journals Hepatic and biochemical repercussions of a polyunsaturated fat-rich hypercaloric and hyperlipidic diet in Wistar rats

2011 ◽  
Vol 48 (2) ◽  
pp. 153-158 ◽  
Author(s):  
Idália M. B. Burlamaqui ◽  
Conceição A. Dornelas ◽  
José Telmo Valença Jr ◽  
Francisco J. C. Mesquita ◽  
Lara B. Veras ◽  
...  
Keyword(s):  
Total Cholesterol ◽  
Wistar Rats ◽  
Average Weight ◽  
Standard Diet ◽  
Polyunsaturated Fat ◽  
Alcoholic Fatty Liver ◽  
The Metabolic Syndrome ◽  
Group I ◽  
Hyperlipidic Diet ◽  
Group Ii

CONTEXT: Non-alcoholic fatty liver disease is characterized by lipid deposits in the hepatocytes and has been associated with obesity, dyslipidemia and type-2 diabetes. It is considered a hepatic manifestation of the metabolic syndrome, of which the main component is insulin resistance leading to hyperinsulinemia and increased production of inflammatory cytokines. Saturated fat promotes hypertriglyceridemia and hyperinsulinemia, reduces levels of high-density cholesterol and increases levels of low-density cholesterol, while polyunsaturated fat is associated with hypolipidemic, antiinflammatory and imunoregulating action. OBJECTIVE: To evaluate the hepatic and biochemical repercussions of a polyunsaturated fat-rich diet in Wistar rats. METHODS: Twenty-two rats were distributed equally in two groups: GI - standard diet (Biobase Bio-tec Ratos e Camundongos®) providing 3.000 kcal/kg and GII - hypercaloric and hyperlipidic diet providing 4.250 kcal/kg (ω-6:ω-3 = 3:1). The animals were euthanized after 23 weeks of experiment. The weight, biochemical parameters and hepatohistological changes were registered. RESULTS: Findings were submitted to variance analysis with the level of statistical significance at 5%. The average weight did not differ significantly between the groups at baseline (P = 0.711), but was greater in Group II by the end of the experiment (P = 0.000). The levels of triglycerides (P = 0.039), total cholesterol (P = 0.015) and HDL (P = 0.005) were higher in Group I than in Group II. Macrovesicular steatosis was significantly more common in Group II than in Group I (P = 0.03). CONCLUSION: Hypercaloric and hyperlipidic diet rich in polyunsaturated fat promotes weight gain and favors the development of hepatic steatosis while reducing serum levels of triglycerides, total cholesterol and HDL.

scholarly journals HEPATOPROTECTIVE EFFECT OF BANANA PEEL FLOUR ON HISTOLOGICAL AND LIVER FUNCTION IN DIABETIC RATS

2021 ◽  
pp. 529-538
Author(s):  
Herlin Ajeng Nurrahma ◽  
Andreanyta Meliala ◽  
Paramita Narwidina ◽  
Sri Herwiyanti
Keyword(s):  
Liver Function ◽  
Diabetic Rats ◽  
Hepatoprotective Effect ◽  
Banana Peel ◽  
Control Group ◽  
Standard Diet ◽  
Hepatic Enzyme ◽  
Alcoholic Fatty Liver ◽  
Group I ◽  
Male Wistar Rats

In diabetes mellitus, non-alcoholic fatty liver disease (NAFLD) is closely linked to hyperglycemia metabolism. This study aimed to find out how a banana peel supplemented diet affected histological and liver function changes in streptozotocin-induced diabetic rats. Vitamins, minerals, dietary fiber, antioxidants, and tryptophan are all contained in banana peel flour (BPF). Serotonin is a neurotransmitter that has been linked to depression and anxiety. This post-test-only control group study was conducted on twenty-five male Wistar rats which were separated into five groups with different treatments. Groups II to V were diabetic rats model groups that consumed standard diet mixed with BPF 0%, 5%, 10%, and 20%, respectively, while group I was a healthy control group fed a standard diet. Hepatic enzyme transaminase (Alanine Aminotransferase-ALT and Aspartate Aminotransferase - AST) and Hematoxylin-Eosin (HE) staining were analyzed with the NAFLD score to examine the liver function and hepatocellular morphology. A change in liver function was observed, as well as a substantial change in the levels of ALT and AST. The NAFLD score with HE staining showed substantial improvements in liver morphology, which was better seen at a 20% BPF dose. The current study supported the hypothesis that BPF had a hepatoprotective effect in diabetic rats, which may be due to the mechanism of controlling the hepatic enzyme transaminase and inducing liver regeneration.

scholarly journals GAMBARAN HISTOLOGIK AORTA TIKUS WISTAR DENGAN DIET LEMAK BABI SETELAH PEMBERIAN EKSTRAK DAUN PEPAYA

2013 ◽  
Vol 5 (1) ◽  
Author(s):  
Friska W. F. Panjaitan ◽  
Marie M. Kaseke ◽  
George N. Tanudjaja
Keyword(s):  
Wistar Rats ◽  
Leaf Extract ◽  
Foam Cells ◽  
Group Iv ◽  
Control Group ◽  
Histological Features ◽  
Group I ◽  
Group Ii ◽  
Lard Diet ◽  
Control Group Group

Abstract: Papaya leaves contain several antioxidants (flavonoid, tanin, and vitamin C) which have antiatherogenic effects that may inhibit the progression of an atherosclerotic lesion. One of the risk factors causing atherosclerosis is the consumption of food containing highly saturated fatty acids e.g. lard. Low density lipoproteins (LDL) accumulate within the intima and then are oxidized (LDL-ox). This LDL-ox is ingested by macrophages, resulting in foam-cell formation (early lesion of atherosclerosis). This study aimed to find out the histological features of the aorta of wistar rats having lard diets without the addition of the papaya leaf extract; having lard diets along with the papaya leaf extract; and having lard diets followed by papaya leaf extract. This was an experimental study on 16 wistar rats divided into 4 groups: group I without treatment (negative control group), group II lard diet for 14 days (positive control group), group III lard diet with papaya leaf extract for 14 days (treatment group I), and group IV lard diet for 14 days, and then followed by papaya leaf extract for 14 days (treatment group II). It was found that the aorta of group I showed adipose cells in the intima and media layers; group II and III showed foam cells in both layers; and group IV showed foam cells in fewer numbers than group II. Conclusion: The aorta histological features of wistar rats given lard diets for 14 days, with or without papaya leaf extract, showed foam cells in the intima and media layers. Papaya leaf extraxt added to lard diets had no effect on decreasing foam cells (no protective effect), meanwhile papaya leaf extract following lard diets showed a reduction of foam cells (therapeutic effect). Keywords: papaya leaf, lard dietary, foam cells, wistar rat.   Abstrak: Daun pepaya mengandung antioksidan (flavonoid, vitamin C) yang berefek anti-aterogenik, sehingga diharapkan dapat menghambat perkembangan lesi aterosklerosis. Salah satu faktor risiko penyebab aterosklerosis yaitu makanan yang berkandungan tinggi asam lemak jenuh, antara lain lemak babi. Konsumsi lemak jenuh berlebihan dapat mengganggu fungsi sel endotel, sehingga lipoprotein berdensitas rendah (LDL) dapat masuk dan menjadi LDL teroksidasi (LDL-oks). Makrofag menangkap LDL-oks dan menjadi sel busa (lesi dini aterosklerosis). Penelitian ini bertujuan untuk mengetahui gambaran histologik aorta tikus wistar dengan diet lemak babi tanpa pemberian ekstrak daun pepaya, diet lemak babi bersamaan pemberian ekstrak daun pepaya, dan setelah diet lemak babi dilanjutkan pemberian ekstrak daun pepaya. Penelitian ini bersifat eksperimental. Subyek penelitian terdiri dari 16 ekor tikus wistar yang dibagi menjadi empat kelompok: kelompok I tanpa perlakuan (kelompok kontrol negatif); kelompok II dengan diet lemak babi selama 14 hari (kelompok kontrol positif); kelompok III dengan diet lemak babi serta pemberian ekstrak daun pepaya selama 14 hari (kelompok perlakuan I); dan kelompok IV dengan diet lemak babi selama 14 hari, dilanjutkan pemberian ekstrak daun pepaya selama 14 hari (kelompok perlakuan II). Hasil penelitian memperlihatkan gambaran histologi aorta kelompok I tampak perlemakan; pada kelompok II terdapat sel-sel busa; pada kelompok III masih terdapat sel-sel busa; dan pada kelompok IV terdapat sel-sel busa, namun dalam jumlah yang lebih sedikit dibandingkan pada gambaran kelompok II. Simpulan: Tikus wistar dengan diet lemak babi selama 14 hari, baik dengan maupun tanpa ekstrak daun pepaya, memperlihatkan gambaran histologik adanya sel-sel busa pada tunika intima dan tunika media aorta. Pemberian ekstrak daun pepaya bersamaan dengan diet lemak babi tidak berefek menurunkan jumlah sel busa (tidak ada efek protektif) sedangkan pemberian ekstrak daun pepaya setelah diet lemak babi berefek mengurangi jumlah sel-sel busa yang terbentuk (efek terapi). Kata kunci: daun pepaya, diet lemak babi, sel-sel busa, tikus wistar.

scholarly journals GAMBARAN MIKROSKOPIK GINJAL TIKUS WISTAR (RATTUS NORVEGICUS) SETELAH DIINDUKSI DENGAN GENTAMISIN

2013 ◽  
Vol 4 (3) ◽  
Author(s):  
Poppy M Lintong ◽  
Carla F Kairupan ◽  
Priska L N Sondakh
Keyword(s):  
Body Weight ◽  
Epithelial Cells ◽  
Wistar Rats ◽  
Periodic Acid ◽  
Basal Membrane ◽  
Tubular Epithelial Cells ◽  
Periodic Acid Schiff ◽  
Group Iii ◽  
Group I ◽  
Group Ii

Abstract: Gentamycin, a frequently used aminoglycoside antibiotics, has a nephrotoxic effect to human beings and animals. The purpose of this research was to find out the microscopic changes of wistar rat kidneys after gentamycin induction. This was an experimental study, using five adult wistar rats, divided into three groups. Group I was the control group; group II consisted of two rats, injected with gentamycin 0,3 ml/day (dose of 60 mg/kg body weight/day) intraperitoneally for seven days; and group III consisted of two rats, injected with gentamycin 0,3 ml/day intraperitoneally for 10 days. Group I and II were terminated at day-8, and group III at day-11. Their kidneys were processed for microscopic slides, stained with hematoxylin eosin and Periodic Acid Schiff. In microscopic evaluation, group II and III showed oedema, necrosis, apoptosis, and basal membrane destruction of tubular epithelial cells. Group III also showed fat vacuoles in these epithelial cells (macrovesicular fatty changes). Conclusion: wistar rats injected with gentamycin 60 mg/kg body weight/day for 7 and 10 days showed oedema, necrosis, apoptosis, and basal membrane destruction of tubular epithelial cells; and macrovesicular fatty changes after 10 days of gentamycin.Key words: gentamycin, necrosis tubular epithelial cells, fatty changesAbstrak: Gentamisin termasuk antibiotik golongan aminoglikosida berspektrum luas yang bersifat nefrotoksik terhadap manusia dan hewan. Tujuan penelitian ini untuk melihat perubahan mikroskopik struktur ginjal tikus Wistar setelah diberikan gentamisin. Metode penelitian eksperimental dengan menggunakan lima ekor tikus Wistar dewasa yang dibagi atas tiga kelompok. Kelompok I tanpa perlakuan; kelompok II terdiri dari dua ekor tikus perlakuan yang diinjeksi dengan gentamisin 0,3 ml/hari (dosis 60 mg/kgBB/hari) secara intraperitonial selama tujuh hari; dan kelompok III terdiri dari dua ekor tikus perlakuan yang diinjeksi dengan gentamisin 0,3 ml/hari secara intraperitonial selama 10 hari. Tikus Wistar kelompok I dan II diteminasi hari ke-8, sedangkan kelompok III diterminasi hari ke-11. Ginjal tikus kelompok I -III kemudian dibuat preparat histopatologik dengan pengecatan rutin hematoksilin eosin dan Periodic Acid Schiff (PAS). Hasil penelitian menunjukkan tikus Wistar perlakuan yang diberikan gentamisin 0,3 ml/hari selama 7 sampai 10 hari secara mikroskopik memperlihatkan pembengkakan, nekrosis, apoptosis, dan destruksi membrana basalis sel epitel tubulus; dan pada hari ke-10 terlihat vakuol-vakuol lemak pada sel epitel sehingga inti terdesak ke tepi (perlemakan makrovesikuler). Simpulan: pemberian gentamisin pada tikus Wistar dengan dosis 60 mg/kg BB/hari selama 7-10 hari menunjukkan pembengkakan, nekrosis, apoptosis sel epitel tubulus, dan membrana basalis tubulus rusak; dan setelah hari ke-10 juga terlihat perlemakan makrovesikuler.Kata kunci: gentamisin, nekrosis sel epitel tubulus, perlemakan makrovesikuler

Effect of Ethanolic Extract of Carica Papaya seeds in Drug-Induced Acute Nephrotoxicity

2021 ◽  
Vol 15 (11) ◽  
pp. 2951-2953
Author(s):  
Sameer Ahmed ◽  
Muhammad Tahir ◽  
Attiya Munir ◽  
Amtul Hafeez ◽  
Sher Afghan Khan ◽  
...  
Keyword(s):  
Carica Papaya ◽  
Ethanolic Extract ◽  
Control Group ◽  
Standard Diet ◽  
Serum Urea ◽  
Once Daily ◽  
Group Iii ◽  
Group I ◽  
Papaya Seeds ◽  
Group Ii

Background: Nephrotoxicity is characterized by raised serum urea and creatinine levels and kidneys being one of the primary organs of drug concentration and excretion are vulnerable to many widely used marketed drugs, including anti-cancer drugs, antibiotics, immunosuppressants, and radio contrast agents, are nephrotoxic. Aim: To determine the nephroprotective effect of ethanolic extract of Carica papaya seeds in Aminoglycoside induced acute nephrotoxicity. Study design: Quasi Experimental study. Methodology: Thirty Sprague Dawley rats were sub divided into 3 groups i.e., I, II and III with 10 rats in each group. Group II and III were administered Aminoglycoside drug; Gentamycin in 80 mg/kg) via intraperitoneal route once daily for 5 consecutive days to induce acute nephrotoxicity. At day 6, nephrotoxicity was confirmed by measuring serum urea and creatinine. Ethanolic extract of Carica papaya seeds (1000 mg/kg) was started once daily through oral route in group III for 5 consecutive days to see the nephroprotective effects of seed extract after causing acute kidney injury. All animals were given standard diet pellets manufactured at NIH. Results: Mean serum urea and creatinine for Group I (Control Group) at day 0 were 24.90 mg/dL ± 1.633 and .750 mg/dL ± .0619 respectively. Mean serum urea and creatinine for Group II (Disease Control Group) was 81.00 mg/dL ± 1.247 and 1.980mg/dL ± .0467 at day 6th. This suggested induction of nephrotoxicity by Gentamycin. Mean serum urea and creatinine for Group III (Ethanolic Extract Treated Group) at 11 day was 72.40mg/dL± .991 and 1.680 mg/dL± .0467 after 5 days treatment with ethanolic extract of Carica papaya seeds. Conclusion: We concluded that ethanolic extract of Carica papaya seeds has significant nephroprotective effects on Aminoglycoside induced acute nephrotoxicity in rats. Keywords: COVID-19, Vaccination, Awareness and Adverse Effects

scholarly journals Cytokeratin 18 as a non invasive marker in diagnosis of NASH and its usefulness in correlation with disease severity in Egyptian patients

QJM ◽  
2020 ◽  
Vol 113 (Supplement_1) ◽  
Author(s):  
M Maher ◽  
H Abdelaziz ◽  
T yossif ◽  
M Ossama
Keyword(s):  
Fatty Liver ◽  
Hepatic Steatosis ◽  
Disease Severity ◽  
P Value ◽  
Cytokeratin 18 ◽  
Alcoholic Fatty Liver ◽  
Non Invasive ◽  
Group I ◽  
Egyptian Patients ◽  
Group Ii

Abstract Background Non-alcoholic fatty liver disease (NAFLD) is one of the types of fatty liver which occurs when exceeive fat is deposited in the liver due to causes other than excessive alcohol use, NAFLD is subdivided into non alcoholic fatty liver (NAFLD) and nonalcoholic steatohepatitis (NASH). In NAFLD, hepatic steatosis is present without evidence of significant inflammation, whereas in NASH, hepatic steatosis is associated with hepatic inflammation Aims To evaluate the role of CK-18 as a non invasive marker in diagnosis of NASH and its usefulness in correlation with disease severity in Egyptian patients. Patients and Methods 60 subjects were divided into 2 groups: group I: including 30 patients with NAFLD, group II: including 30 matched healthy controls Diagnosis of NASH and its discrimination from NAFL was done by fibroscan. CK-18 level in plasma was measured for all subjects using ELISA. Results CK-18 was significantly elevated in patients of group I in comparison to group II, with mean ± SD: 460 ± 279, 167 ± 56 and 149 ± 57, respectively, and P value: 0.001. with mean ±SD: 59.6± 28, when compared with control group (with 23±8) P value < 0.001. ROC curve between Cases and Control regard CK18 with Area Under the Curve (AUC): 0.925. Cutoff > 30 ug/l With Sensitivity: 86.67% & Specificity: 83.33%. Ck-18 was found to correlate with steatosis and fibrosis assessed by fibroscan with P value< 0.001. Conclusion Measurement of serum cytokeratin 18 fragment levels (CK18) correlate with the Fibroscan (Transient Elastograph) as a non invasive tests in diagnosis & prognosis of non alcoholic fatty liver diseases (NAFLD).

scholarly journals Effects of sodium hyaluronate and carboxymethylcellulose membrane on collagen and fibroblast formation in bowel suture healing: experimental study in rats

2005 ◽  
Vol 20 (1) ◽  
pp. 15-21 ◽  
Author(s):  
Antônio Carlos Perez ◽  
Delcio Matos ◽  
Edna Frasson de Souza Montero ◽  
Heitor Soares de Souza ◽  
Fábio Colagrossi Paes Barbosa ◽  
...  
Keyword(s):  
Experimental Study ◽  
Wistar Rats ◽  
Sodium Hyaluronate ◽  
Morbidity And Mortality ◽  
Collagen Formation ◽  
Significant Variance ◽  
Group I ◽  
Male Wistar Rats ◽  
Group Ii ◽  
Observation Period

PURPOSE: To analyze the effects of sodium hyaluronate and carboxymethylcellulose membrane on collagen and fibroblast formation in bowel suture healing in rats. METHODS: 48 male Wistar rats, weighing 250 to 343g, were randomized into two groups: group I - bowel suture without applying a biologically absorbable membrane and group II - bowel suture with application of an absorbable membrane. The two groups were divided into subgroups of 3, 14 and 30 days of observation, with 8 rats in each subgroup. All were sacrificed after the end of the observation period. RESULTS: No morbidity or mortality was observed during the experiment. The amounts of collagen in group I were 23.4%, 72.1% and 67.6% and in group II were 22.5%, 52.5% and 51.6%, for the subgroups of 3, 14 and 30 days, respectively. Comparison between groups showed that the 14-day (p=0.0013) and 30-day (p=0.0587) subgroups had significant variance, with larger collagen zones in animals in which the membrane was not applied. However, with regard to fibroblasts, group I had 2%, 13% and 8% and group II had 2%, 10% and 8%, for the 3-day (p=1.0), 14-day (p=0.3184) and 30-day (p=0.5995) subgroups, respectively, showing no significant variance. CONCLUSION: The use of the biologically absorbable membrane cause a decrease in collagen formation, while not altering the number of fibroblasts, in bowel suture healing in rats, without increased morbidity and mortality.

scholarly journals The Effect of Dietary Camelina Sativa Oil on Quality of Broiler Chicken Meat / Wpływ Udziału Oleju Z Lnianki Siewnej (Camelina Sativa) W Dawkach Dla Kurcząt Rzeźnych Na Jakość Mięsa

2013 ◽  
Vol 13 (4) ◽  
pp. 869-882 ◽  
Author(s):  
Mariusz P. Pietras ◽  
Sylwia Orczewska-Dudek
Keyword(s):  
Total Cholesterol ◽  
Total Protein ◽  
Broiler Chickens ◽  
Rapeseed Oil ◽  
Camelina Sativa ◽  
Control Group ◽  
Breast Meat ◽  
Camelina Oil ◽  
Group I ◽  
Group Ii

Abstract An experiment was conducted on 600 broiler chickens to determine the effect of using Camelina sativa oil as a dietary component on meat quality indicators. Broiler chickens were raised on litter under standard conditions of feeding and maintenance. In the second period of rearing, the control group (I) received a diet containing 6% rapeseed oil. Experimental groups were fed on a diet containing 3% rapeseed oil and 3% Camelina sativa oil (group II) and 6% camelina oil (group III). At the end of rearing 8 chickens from each group were slaughtered. A simplified analysis of the carcasses was conducted. Blood samples were taken to determine the content of total fat, triglycerides, total cholesterol and its fractions. Muscle samples were analysed for the content of dry matter, total protein and crude fat, fatty acid composition and malonic aldehyde (TBA). The meat was subjected to sensory evaluation. It was found that the introduction of Camelina sativa oil to the grower diet for broiler chickens does not have a negative effect on rearing parameters and carcass quality. What was observed was a tendency to reduce the proportion of abdominal fat in the carcass with increasing level of oil used in compound feed and increases in the total protein content of breast meat in group II receiving 3% of camelina oil. The addition of 6% of Camelina sativa oil to compound feed significantly decreased the content of total cholesterol and its fractions in the blood plasma of chickens in comparison to the other groups. The introduction of 3% and 6% of Camelina sativa oil to chicken diets enriched the breast meat in n-3 PUFA, mainly α-linolenic acid (ALA) and did not impair the flavour qualities of the cooked meat. The meat from chickens fed on a diet containing 6% of camelina oil was characterized by a greater increase of ALA.

scholarly journals Biochemical Effects of Matured Stem Extract of Opuntia Dillenii in Male Wistar Rats

2018 ◽  
Vol 11 (1) ◽  
pp. 49-58
Author(s):  
Uche C. Njoku ◽  
Benjamin A. Amadi ◽  
Peter U. Amadi ◽  
Onyebuchi E. Ezendiokwere ◽  
Idongesit E. Archibong
Keyword(s):  
Wistar Rats ◽  
Density Lipoprotein ◽  
Control Group ◽  
Group Iii ◽  
Therapeutic Benefits ◽  
Group I ◽  
Male Wistar Rats ◽  
Stem Extract ◽  
Group Ii ◽  
Opuntia Dillenii

Summary The effect of aqueous matured stem extract of Opuntia dillenii on selected biochemical parameters in Male Wistar rats was explored. Standard analytical methods were applied. Forty Wistar rats (80-100g) were used in the animal studies, separated into four groups. The control group was solely administered normal feed and saline, group I was administered 100mgkg−1 of the extract, group II received 300mgkg−1 of the extract and group III received 500 mg/kg−1 of the extract. A significant increase (p<0.05) in the activities of alanine aminotransferase (ALT) and alkaline phosphatase was observed in group II and III rats, as compared with the controls. A significant decrease in urea and creatinine concentrations was found only in group III rats against the controls. Also, a significant (p<0.05) decrease in triglyceride, total cholesterol, and low density lipoprotein (LDL)-cholesterol was seen in group II and group III rats when compared with the control. The hematological evaluation revealed a significant (p<0.05) decrease in red blood cell and hemoglobin levels in group III rats when compared with the control. The findings showed both beneficial and toxicological effects of the plant. Hence, for optimal therapeutic benefits, a further toxicological survey could still be carried out perhaps at higher doses.

scholarly journals GAMBARAN HISTOPATOLOGI HATI TIKUS WISTAR YANG DIBERIKAN JUS TOMAT (Solanum Lycopersicum) PASCA KERUSAKAN HATI WISTAR YANG DIINDUKSI KARBON TETRAKLORIDA (CCl4)

2013 ◽  
Vol 1 (3) ◽  
Author(s):  
Eka Sari Tappi ◽  
Poppy Lintong ◽  
Lily Loho
Keyword(s):  
Carbon Tetrachloride ◽  
Wistar Rats ◽  
Liver Cells ◽  
The Body ◽  
Tomato Juice ◽  
Control Group ◽  
Histopathological Changes ◽  
Group Iii ◽  
Group I ◽  
Group Ii

Abstract: Liver is the largest organ in the abdominal cavity. As the center of metabolism in the body, liver is potentially damaged by exposure of toxic substances, inter alia carbon tetrachloride (CCl4). Metabolism of carbon tetrachloride (CCl4) produces CCl3 free radicals that can damage the liver. In Indonesia, there are a lot of natural ingredients that have antioxidant properties, such as tomato. Lycopene in tomatoes contains antioxidant compounds that can prevent damages due to free radical. This study aimed to obtain liver histopathological changes of wistar rats fed with tomato juice after being induced of carbon tetrachloride (CCl4). This was an experimental study, using 10 wistar rats which were divided into 4 groups. Group I was the negative control; group II was induced with CCl4 0,05 cc/day and was terminated on day 6; group III was induced with CCl4 0,05 cc/day and was given tomato juice 3 ml/day, and terminated on day 13; group IV was induced by CCl4 0,05 cc/day, given regular pellets, and terminated on day 13. The results showed that group II had histopathological changes of the liver indicating fatty liver, meanwhile group III showed regeneration of nearly all liver cells. Conclusion: Administration of tomato juice after the induction of 3 ml carbon tetrachloride (CCl4) for 7 day showed regeneration of almost all liver cells. Keywords: histopathological changes of the liver, carbon tetrachloride, tomato juice.   Abstrak: Hati merupakan organ terbesar dalam rongga abdomen, dan pusat metabolisme tubuh dengan fungsi yang sangat kompleks dan sangat berpotensi mengalami kerusakan akibat terpapar oleh bahan-bahan toksik, salah satunya yaitu karbon tertraklorida (CCL4). Metabolisme CCl4 menghasilkan radikal bebas CCl3 yang dapat merusak hati. Di Indonesia terdapat  banyak sekali bahan-bahan alami yang mempunyai kandungan antioksidan, salah satunya yaitu tomat. Tomat mengandung senyawa likopen sebagai antioksidan yang dapat mencegah kerusakan jaringan akibat radikal bebas. Penelitian ini bertujuan untuk mendapatkan gambaran histopatologi hati tikus wistar yang diberi jus tomat pasca induksi karbon tetraklorida (CCl4). Metode penelitian ialah eksperimental. Sampel sebanyak 10 ekor tikus wistar yang dibagi dalam 4 kelompok. Kelompok I sebagai kontrol negatif; kelompok II diinduksi CCl4 0,05cc/perhari dan diterminasi hari ke-6; kelompok III diinduksi CCl4 0,05 cc/hari kemudian diberikan jus tomat 3ml/hari;  dan kelompok IV diinduksi CCl4 0,05 cc/hari kemudian diberikan pelet biasa dan diterminasi hari ke-13. Hasil penelitian menunjukkan pada pemberian CCl4 pada tikus wistar selama 5 hari terdapat gambaran morfologik perlemakan sel hati. Pemberian jus tomat dosis 3 ml pasca induksi karbon tetraklorida (CCl4) menunjukkan terjadinya regenerasi pada hampir seluruh sel-sel hati. Simpulan: Pemberian jus tomat dosis 3 ml pasca induksi karbon tetraklorida (CCl4) selama 7 hari menunjukkan regenerasi pada hampir seluruh sel-sel hati. Kata kunci: gambaran histopatologi hati, karbon tetraklorida, jus tomat.

scholarly journals The Hepatoprotector Effect of Uncaria gambir Roxb Extract in Wistar Rats Induced by Paracetamol

2020 ◽  
Vol 10 (6-s) ◽  
pp. 61-66
Author(s):  
, Suparni ◽  
, Musthari ◽  
Liza Mutia ◽  
Mangoloi Sinurat ◽  
Siti Syarifah ◽  
...  
Keyword(s):  
Wistar Rats ◽  
Chronic Administration ◽  
Liver Cells ◽  
Group Iv ◽  
Drug Induced ◽  
Significant Differentiation ◽  
Group Iii ◽  
Hepatoprotector Activity ◽  
Group I ◽  
Group Ii

Background: Drug induced liver injury (DILI) is known as the damage of liver cells due to chronic administrations of drug. The chronic administration of paracetamol could be trigger the damage of liver cells.The hepatoprotector agents are still limited worldwide.  Gambier(Uncaria gambir Roxb) is an Indonesia’traditional medicine which have many benefits as antioxidant, antiseptic, antidiarrhoea, etc  that commonly used in society.  Method: The present study was conducted to investigate the hepatoprotector effect ofgambier in wistar rats induced by paracetamol.  The wistar rats were divided into seven groups and received the treatment orally for 12 days. Group I (aquadest), II(curcuma,400 mg/kgBW),III (gambier,26 mg/200gr), IV(gambier, 53 mg/200gr), V(gambier,106 mg/200gr),VI(gambier,212 mg/200gr) and VII(gambier,424mg/200gr). Termination, blood and liver organ collection were done after all group induced by paracetamol for two days. Histopatology changes of liver were examined using Hematoxycilline (HE) staining. AST and ALT levels were analyzed. Results: There were significant differentiation of AST levels among the groups, especially between group I and group IV and between group II and group IV. The ALT levels were statistically significant between group II and group V using Mann-Whitney test (p<0,05). In histopatology examination, there were significant differentiation between group I with another group, not only group II but also group III-VII (p<0,05). In the treatment group, group III and IV had been showed the improvement of liver cells damage than group I by using One-way Annova, post hoc Bonferroni (p<0,05). Conclusion: Uncaria gambir Roxb has hepatoprotector activity start at dose 53 mg/200grBWin rats.  The hepatoprotector activity was not superior than curcuma.  Keywords: hepatoprotector, Uncaria gambir Roxb, AST,ALT,histopatology

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