Evaluation of Nephroprotective Effects of Hydroalcoholic Extract of Cyperus scariosus Linn. in Gentamicin-induced Acute Kidney Injury in Wistar Albino Rats

2016 ◽  
Vol 11 (3) ◽  
Author(s):  
Khushboo K. Gajjar ◽  
Amol S. Aiwale ◽  
Ashish P. Anovadiya ◽  
Ashvin V. Mevada ◽  
Seema N. Baxi ◽  
...  
Keyword(s):  
Acute Kidney Injury ◽  
Kidney Injury ◽  
Albino Rats ◽  
Hydroalcoholic Extract ◽  
Wistar Albino Rats

scholarly journals Resuscitation with PEGylated carboxyhemoglobin preserves renal cortical oxygenation and improves skeletal muscle microcirculatory flow during endotoxemia

2020 ◽  
Vol 318 (5) ◽  
pp. F1271-F1283 ◽  
Author(s):  
Philippe Guerci ◽  
Bülent Ergin ◽  
Aslı Kandil ◽  
Yasin Ince ◽  
Paul Heeman ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Acute Kidney Injury ◽  
Plasma Levels ◽  
Kidney Injury ◽  
Albino Rats ◽  
Heme Oxygenase 1 ◽  
Animal Group ◽  
Phosphorescence Quenching ◽  
Time Control ◽  
Microcirculatory Flow

PEGylated carboxyhemoglobin (PEGHbCO), which has carbon monoxide-releasing properties and plasma expansion and oxygen-carrying properties, may improve both skeletal microcirculatory flow and renal cortical microcirculatory Po2 (CµPo2) and, subsequently, limit endotoxemia-induced acute kidney injury. Anesthetized, ventilated Wistar albino rats ( n = 44) underwent endotoxemic shock. CµPo2 was measured in exposed kidneys using a phosphorescence-quenching method. Rats were randomly assigned to the following five groups: 1) unresuscitated lipopolysaccharide (LPS), 2) LPS + Ringer’s acetate (RA), 3) LPS + RA + 0.5 µg·kg·−1min−1 norepinephrine (NE), 4) LPS + RA + 320 mg/kg PEGHbCO, and 5) LPS + RA + PEGHbCO + NE. The total volume was 30 mL/kg in each group. A time control animal group was used. Skeletal muscle microcirculation was assessed by handheld intravital microscopy. Kidney immunohistochemistry and myeloperoxidase-stained leukocytes in glomerular and peritubular areas were analyzed. Endotoxemia-induced histological damage was assessed. Plasma levels of IL-6, heme oxygenase-1, malondialdehyde, and syndecan-1 were assessed by ELISA. CµPo2 was higher in the LPS + RA + PEGHbCO-resuscitated group, at 35 ± 6mmHg compared with 21 ± 12 mmHg for the LPS+RA group [mean difference: −13.53, 95% confidence interval: (−26.35; −0.7156), P = 0.035]. The number of nonflowing, intermittent, or sluggish capillaries was smaller in groups infused with PEGHbCO compared with RA alone ( P < 0.05), while the number of normally perfused vessels was greater ( P < 0.05). The addition of NE did not further improve CµPo2 or microcirculatory parameters. Endotoxemia-induced kidney immunohistochemistry and histological alterations were not mitigated by PEGHbCO 1 h after resuscitation. Renal leukocyte infiltration and plasma levels of biomarkers were similar across groups. PEGHbCO enhanced CµPo2 while restoring skeletal muscle microcirculatory flow in previously nonflowing capillaries. PEGHbCO should be further evaluated as a resuscitation fluid in mid- to long-term models of sepsis-induced acute kidney injury.

scholarly journals The Effects of Vitamin D on Gentamicin-Induced Acute Kidney Injury in Experimental Rat Model

2013 ◽  
Vol 2013 ◽  
pp. 1-7 ◽  
Author(s):  
Ender Hur ◽  
Alev Garip ◽  
Asuman Camyar ◽  
Sibel Ilgun ◽  
Melih Ozisik ◽  
...  
Keyword(s):  
Vitamin D ◽  
Acute Kidney Injury ◽  
Rat Model ◽  
Urine Volume ◽  
Kidney Injury ◽  
Control Group ◽  
Albino Rats ◽  
Tubular Injury ◽  
Renal Histology ◽  
Experimental Rat Model

Introduction. Acute kidney injury (AKI) pathogenesis is complex. Findings of gentamicin nephrotoxicity are seen in 30% of the AKI patients. Vitamin D has proven to be effective on renin expression, inflammatory response, oxidative stress, apoptosis, and atherosclerosis. We aimed to investigate the effect of vitamin D in an experimental rat model of gentamicin-induced AKI.Methods. Thirty nonuremic Wistar albino rats were divided into 3 groups: Control group, 1 mL saline intramuscular (im) daily; Genta group, gentamicin 100 mg/kg/day (im); Genta + vitamin D, gentamicin 100 mg/kg/day (im) in addition to 1α, 25 (OH)2D30.4 mcg/kg/day subcutaneously for 8 days. Blood pressures and 24-hour urine were measured. Blood urea and creatinine levels and urine tubular injury markers were measured. Renal histology was semiquantitatively assessed.Results. Urea, creatinine and urine neutrophil gelatinase-associated lipocalin, and kidney injury molecule-1 were all increased in Genta group indicating AKI model. Systolic blood pressure decreased, but urine volume and glutathione increased in Genta + Vit D group compared to Control group. Histological scores indicating tubular injury increased in Genta and Genta + Vit D groups.Conclusions. Vitamin D does not seem to be effective on histological findings although it has some beneficial effects via RAS system and a promising effect on antioxidant system.

scholarly journals PREVENTIVE EFFECT OF PHASEOLUS VULGARIS SEED COATS ON PENTYLENETETRAZOLE (PTZ) INDUCED KINDLING AND BEHAVIORAL COMORBIDITIES

2021 ◽  
Vol 3 (4) ◽  
pp. 20-25
Author(s):  
Sarika Chaudhary ◽  
Sagarika Majhi ◽  
Madhu Verma
Keyword(s):  
Phaseolus Vulgaris ◽  
Albino Rats ◽  
Glutathione Level ◽  
Preventive Effect ◽  
Hydroalcoholic Extract ◽  
Nitric Oxide Level ◽  
Anti Oxidant ◽  
Wistar Albino Rats ◽  
The Brain ◽  
Seed Coats

Introduction: To investigate the preventive effect of Phaseolus vulgaris seed coats on pentylenetetrazole-induced kindling and behavioral comorbidities. Material and Methods: Thirty Wistar albino rats were categorized into five groups. The first group received regular saline (0.9 % w/v NaCl) p.o.; the second group received PTZ (35 mg/kg b.w.) i.p.; the third group received valproic acid (200 mg/kg b.w.) p.o.; the fourth group received P. vulgaris extract (100 mg/kg b.w.) p.o.; the fifth group received P. vulgaris extract (200 mg/kg b.w.) p.o. on an alternate day for 21 days. PTZ improved lipid peroxidase levels, decreased Glutathione level, decreased superoxide dismutase activity, increased Nitric Oxide level. Result: This study revealed that P. vulgaris (Hydroalcoholic extract) increased the anti-oxidant level of both 100 mg/kg and 200 mg/kg compared to the PTZ category. Histopathological findings revealed that the hippocampal section of the brain of rats receiving P. vulgaris extract had improved relative to the receiving PTZ group. Conclusion: Based on the result, it is proposed that Phaseolus vulgaris has anti-oxidant properties. This is useful for the treatment of epilepsy.

scholarly journals Nephroprotective Activity of Ethanolic Extract of Carissa carandas Leaves against Gentamicin-Induced Acute Kidney Injury in Wistar Albino Rats

2021 ◽  
pp. 279-286
Author(s):  
V. V. Rajesham ◽  
Hima Sowmya Kandoti ◽  
Mitta Raghavendra ◽  
P. Roshan Ali ◽  
T. Rama Rao
Keyword(s):  
Kidney Injury ◽  
Ethanolic Extract ◽  
Albino Rats ◽  
Oxidative Stress Markers ◽  
Disease Group ◽  
Stress Markers ◽  
Carissa Carandas ◽  
Nephroprotective Activity ◽  
Wistar Albino Rats ◽  
Histopathological Studies

The present study was aimed to evaluate the nephroprotective activity of ethanolic extract of Carissa carandas Linn. Leaves (EECC) in Gentamicin-induced nephrotoxicity in Wistar albino rats. The renal damage was induced by Gentamicin (80mg/kg body weight, i.p.). Nephroprotective activity was investigated by the administration of EECC at two different doses (100 and 200mg/kg body weight, p.o) for 28 days and by assessing serum parameters, renal oxidative stress markers and histopathological studies. Gentamicin-treated animals showed an increase in serum creatinine, uric acid, urea, and malondialdehyde (MDA) levels and decrease in total protein, reduced glutathione (GSH), and catalase(CAT) compared to normal control animals, which indicates severe nephrotoxicity. Histopathological studies of kidney Gentamicin-treated animals showed extensive acute tubular necrosis and peri-tubular inflammation. Administration of EECC showed a significant improvement (p<0.05) in biochemical and oxidative stress markers compared to the disease group. EECC treated groups showed better histological appearance when compared to the disease group. Ethanolic extract of Carissa carandas Linn. Leaves showed significant nephroprotective activity against gentamicin-induced acute kidney injury.

scholarly journals Melatonin mitigates cisplatin-induced acute kidney injury through regulation of the heat shock proteins expressions

2021 ◽  
Author(s):  
Ali Tuğrul Akin ◽  
Murat Unsal ◽  
Tayfun Ceylan ◽  
Emin Kaymak ◽  
Emel Ozturk ◽  
...  
Keyword(s):  
Acute Kidney Injury ◽  
Uric Acid ◽  
Heat Shock ◽  
Heat Shock Proteins ◽  
Blood Serum ◽  
Renal Cortex ◽  
Kidney Injury ◽  
Control Group ◽  
Albino Rats ◽  
Shock Proteins

Abstract Purpose. To determine the protective effects of melatonin (MEL) in acute kidney injury (AKI) induced by Cisplatin (CP), widely used as a chemotherapeutic in the treatment of many cancer types, via assessment of heat-shock proteins (HSPs) levels in rats. Methods. Total 40 Wistar albino rats were divided into four groups: Control (n = 10), MEL (n = 10, 10 mg/kg/i.p melatonin for 8 days), CP (n = 10, 7 mg/kg/i.p cisplatin at the 5th day), and CP + MEL (n = 10, 10 mg/kg/i.p melatonin for 8 days and 7 mg/kg/i.p cisplatin at the 5th day). After administrations, animals were sacrificed, and kidney tissues were extracted. Histopathological changes were evaluated and glomerular and tubular immunoreactivities of HSP47, HSP60, HSP7, and HSP90 in renal cortex were detected by immunohistochemistry. Moreover, blood serum BUN, creatinine and uric acid levels were measured to assess of kidney function. Results. CP group showed histopathological deterioration compared to Control group and MEL treatment attenuated this damage. When compared with Control and MEL groups, an increase in HSPs immunoreactivities in renal cortex and blood serum BUN, creatinine and uric acid levels were observed in the CP group. Furthermore, an improvement was observed in the CP + MEL in terms of these parameters compared to the CP group. Conclusion. According to our results, MEL could exert a significant protective effect to ameliorate CP-induced AKI via regulation of heat-shock protein expressions.

PHYTOREMEDIAL POTENTIAL OF HYDROALCOHOL EXTRACT OF SEEDS OF SORGHUM BICOLOR AGAINST DRUG-INDUCED NEPHROTOXICITY

INDIAN DRUGS ◽  
2021 ◽  
Vol 58 (07) ◽  
pp. 46-52
Author(s):  
Sreedevi Adikay ◽  
Sai Sruthi Kaveripakam ◽  
Keyword(s):  
Sorghum Bicolor ◽  
Albino Rats ◽  
Prophylactic Regimen ◽  
Drug Induced ◽  
Hydroalcoholic Extract ◽  
Novel Approach ◽  
Nephroprotective Activity ◽  
Wistar Albino Rats ◽  
Immunohistochemical Studies ◽  
The Impact

The gravity of the impact of drug induced nephrotoxicity is more prominent in society. The present study was undertaken to evaluate the potential of hydroalcoholic extract of seeds of Sorghum bicolor against cisplatin and doxorubicin- induced nephrotoxicity in Wistar albino rats. The nephrotoxicity was modeled by intraperitoneal injection of cisplatin (5 mg/kg b.w.) in cisplatin model and doxorubicin (15 mg/kg b.w.) in doxorubicin-induced model in rats. Nephroprotection of hydroalcoholic extract of seeds of S. bicolor was evaluated at two different doses of 200 and 400mg/kg b.w. The nephroprotective activity was assessed by the determination of various serum and urinary parameters, anti-oxidant studies, histological and immunohistochemical studies. The results indicated that injection of cisplatin and doxorubicin led to marked nephrotoxicity in animals. Treatment with extract in cisplatin-induced model resulted in significant nephroprotective activity in a curative regimen whereas in prophylactic regimen the extract prevented the induction of nephrotoxicity only up to a considerable level. But the extract failed to attenuate the doxorubicin induced nephrotoxicity, as evident by biochemical, histological and immunohistochemical studies. From the findings, it is concluded that the seeds of S. bicolor can be used as a novel approach in the treatment of cisplatin-induced nephrotoxicity.

scholarly journals EFFECT OF ARTHROSPIRA PLATENSIS AS A FOOD SUPPLEMENT AGAINST SODIUM FLUORIDEINDUCED INTOXICATION ON SOFT TISSUES OF MALE WISTAR ALBINO RATS

2017 ◽  
Vol 10 (11) ◽  
pp. 133
Author(s):  
Ravindra Reddy Kandula ◽  
Narasimha Jayaveera Korlakunta ◽  
Raghavendra Mitta
Keyword(s):  
Body Weight ◽  
Soft Tissues ◽  
Radical Scavenging ◽  
Arthrospira Platensis ◽  
Albino Rats ◽  
Hydroalcoholic Extract ◽  
Group I ◽  
Liver And Kidney ◽  
Wistar Albino Rats

  Objective: The objective of the present study was to explore the in vitro antioxidant and effect of hydroalcoholic extract of Arthrospira platensis (ASP) against sodium fluoride (NaF)-induced toxic effects on soft tissues (heart, liver, and kidney).Methods: In vitro antioxidant activity was assessed using 1,1-diphenyl-2-picrylhydrazyl radical scavenging, 2,2’-azino-bis(3-ethylbenzothiazoline- 6-sulphonic acid) radical scavenging, metal chelation, total antioxidant and reducing power assays, and total flavonoid and phenol content. In this study, 36 male Wistar albino rats were divided into six groups of six animals each. Animals were served as Group I - normal control, Group II - toxic control, and Groups III, IV, and V - treatment groups, which received hydroalcoholic extract of ASP at doses of 100, 200, and 400 mg/kg body weight (p.o), respectively. Group VI served as plant control received hydroalcoholic extract of ASP at a dose of 400 mg/kg body weight (p.o). All groups except Groups I and VI received NaF (100 ppm) through drinking water for 30 days.Results: Results showed that intoxication of NaF caused significant elevation of serum biomarkers of the heart, liver, and kidney and altered tissue oxidative stress markers’ levels. Administration of hydroalcoholic extract of ASP significantly normalized elevated serum levels of heart (p<0.001), liver (p<0.001, p<0.05), and kidney biomarkers (p<0.001). Decrease in lipid peroxidation and increase in reduced glutathione and catalase enzyme levels in a dose-dependent manner were observed in soft tissues (p<0.001, p<0.01, and p<0.05).Conclusion: The study revealed that ASP has good antioxidant and mitigative action against NaF intoxication on soft tissues.

scholarly journals Protective Effect of Cucurbita maxima against Maximal Electroshock Induced Convulsions

2021 ◽  
pp. 91-101
Author(s):  
Nagnath R. Kadam ◽  
P. K. Mohanty ◽  
Ashish Jain
Keyword(s):  
Protective Effect ◽  
Albino Rats ◽  
Anticonvulsant Effect ◽  
Maximal Electroshock ◽  
Cucurbita Maxima ◽  
Hydroalcoholic Extract ◽  
Indigenous Plant ◽  
Wistar Albino Rats ◽  
Electroshock Seizures ◽  
Different Doses

In the present investigation, an indigenous plant, Cucurbita maxima was studied for its protective effect against maximal electroshock (MES) induced convulsions in Wistar albino rats. The rats were pretreated with different doses (100, 200, 400 mg/kg) of hydroalcoholic extract of seeds of Cucurbita maxima for 14 days and then, they were subjected to maximal electroshock seizures (40 mA for 0.2 sec) treatment. Hydroalcoholic extract of Cucurbita maxima seeds at 200 and 400 mg/kg doses significantly reduced the duration of hind limb extension along with the protection of rats against maximal electroshock induced seizures. The reference standard i.e., phenytoin (20 mg/kg) provided complete protection. Thus, present study revealed anticonvulsant effect of Cucurbita maxima against maximal electroshock-induced convulsions in rats.

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