scholarly journals Fermentasi Jerami Padi Menggunakan White rot fungi dan Suplementasi Saccharomyces cerevisiae Pengaruhnya terhadap Kecernaan Nutrien Secara In Vitro

2012 ◽  
Vol 12 (2) ◽  
pp. 1-6
Author(s):  
Wardhana Suryapratama ◽  
Fransisca Maria Suhartati
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Rice Straw ◽  
Phanerochaete Chrysosporium ◽  
White Rot Fungi ◽  
Nutrient Content ◽  
Experimental Methods ◽  
White Rot ◽  
Randomized Design ◽  
Completely Randomized Design

The influence of rice straw fermentation using white rot fungi and saccharomyces cerevisiae supplementation on in vitro nutrient digestibilityABSTRACT. An experiment to investigate the effect of rice straw fermented using white rot fungi and Saccharomyces cerevisiae supplementation on nutrient digestibility In Vitro had been implemented in two phases. The first experiments undertaken to make rice straw fermentation, using experimental methods with a Completely Randomized Design. As the treatment were White rot fungi (Phanerochaete chrysosporium) 0, 5 and 10 g/kg of rice straw (DM basis). Each treatment was repeated six times, so there are 18 experimental units. The variables measured included nutrient content of rice straw. A second experiment carried out in vitro to test the best rice straw fermentation results of the first experiment, using experimental methods, with a Completely Randomized Design. As the treatment were the supplementation of Saccharomyces cerevisiae (0, 2, 4% of the weight of fermented rice straw, DM basis). The variables measured included digestibility of dry matter, organic matter, cellulose and lignin digestibility of feed containing fermented straw. The data obtained were analyzed using analysis of variance test followed by Orthogonal Polynomials. The results can be concluded that the fermented rice straw using Phanerochaete chrysosporium 10 g/kg of rice straw is the best nutrient content. Saccharomyces cerevisiae supplementation on feed that contains fermented rice straw using Phanerochaete chrysosporium 10 g/kg rice straw is 2%.

scholarly journals Potensi fungi pelapuk putih asal lingkungan tropik untuk bioremediasi herbisida The potential white-rot fungi native of tropical environment for herbicides bioremediation

2016 ◽  
Vol 75 (1) ◽  
Author(s):  
Laksmita Prima SANTI ◽  
Lisdar Idwan SUDIRMAN ◽  
Didiek Hadjar GOENADI
Keyword(s):  
Laboratory Experiment ◽  
Phanerochaete Chrysosporium ◽  
White Rot Fungi ◽  
Tropical Environment ◽  
White Rot ◽  
Malt Extract ◽  
Ceriporiopsis Subvermispora ◽  
Pleurocybella Porrigens

SummaryFungal treatment by using white-rot fungito reduce a wide variety of herbicide com-pounds is a specialized bioremediation pro-cess. A laboratory experiment was conductedto determine the ability of Phanerochaetechrysosporium, Ceriporiopsis subvermispora,and Pleurocybella porrigens and seven white-rot fungi isolated from a native of tropicalenvironment to grow on yeast malt extractglucose (YMG) agar containing highconcentration of (I) 2,4-dichlorophenoxy aceticacid, (R) glyphosate, and (G) paraquat. Thedata indicated that P. chrysosporium couldgrow on YMG media containing 5000 ppm of(I) 2,4-D, whereas BPBPI 02/04 isolate onYMG 250 ppm of (R) glyphosate or (G)paraquat. Relative values of growth inhibitionof these fungi are 81.1; 27.8; and 50.0%respectively. Biodegradation capability ofherbicides by candidate inoculants in soil-sandmedia was also determined in greenhouseexperiment by using peanut, sorghum, corn,and Borreria alata as bio-indicators. Peanutand B. alata were found to be the bestresponsive seedlings as bio-indicator on thepresence of (I) 2,4-D herbicide in soil-sandmedia.RingkasanTeknologi bioremediasi dengan fungipelapuk putih (FPP) digunakan untuk me-reduksi sejumlah senyawa herbisida. Kegiatanpenelitian yang dilakukan di laboratoriumbertujuan untuk mengetahui kemampuan tum-buh Phanerochaete chrysosporium, Ceripo-riopsis subvermispora, dan Pleurocybellaporrigens serta tujuh isolat FPP yang diperolehdari lingkungan tropik secara in vitro padamedium agar yeast malt extract glucose(YMG) yang mengandung (I) 2,4-dikloro-fenoksi asam asetat, (R) glifosat, dan (G)parakuat konsentrasi tinggi. Dari data yangdiperoleh, diketahui bahwa Ph. chrysosporiummemiliki kemampuan tumbuh dalam mediumpadat YMG yang mengandung 5000 ppm (I)2,4-D dan isolat BPBPI 02/04 pada 250 ppm(R) glifosat dan (G) parakuat dengan nilaihambatan pertumbuhan relatif terhadap kontrol(HPR) masing-masing 81,1; 27,8; dan 50,0%.Pengujian isolat terpilih terhadap kemampuanmendegradasi herbisida di dalam mediumtanah dan pasir juga dilakukan di rumah kacadengan menggunakan kacang tanah, sorgum,jagung, dan Boreria alata sebagai bioindikator.Kacang tanah dan B. alata memberikan responterbaik terhadap keberadaan herbisida (I) 2,4-Ddi dalam medium tanah dan pasir .

scholarly journals Effect of Ligninolytic Axenic and Coculture White-Rot Fungi on Rice Straw Chemical and In Vitro Fermentation Characteristics.

2021 ◽  
Author(s):  
Osmond Datsomor ◽  
Zhao Gou-qi ◽  
Lin Miao
Keyword(s):  
Rice Straw ◽  
Volatile Fatty Acids ◽  
Nutritive Value ◽  
Lignin Content ◽  
Axenic Culture ◽  
White Rot Fungi ◽  
White Rot ◽  
In Vitro Fermentation ◽  
Dry Matter Digestibility

Abstract The objective of this study was to investigate the axenic culture of Pleurotus ostreatus, Phanerochaete chrysosporium, and the coculture (P. chrysosporium and P. ostreatus) for their potential to break down lignin and to enhance the rumen fermentability of rice straw. Rice straw was fermented by two lignin-degrading fungi, namely, P. ostreatus, P. chrysosporium, and coculture (P. ostreatus and P. chrysosporium) under solid-state fermentation (SSF). The coculture exhibited a mutual intermingling plus inhibition interaction. Coculture treated straw had a lower lignin content (5.26%) compared to P. chrysosporium axenic treated straw (6.18%), although P. ostreatus axenic treated straw was the lowest (3.27%). The polysaccharide content of coculture treated straw was higher than P. chrysosporium axenic treated straw, although smaller than P. ostreatus. P. ostreatus and coculture suitably delignified rice straw without adversely affecting cellulose. Treatment of rice straw with coculture improved in vitro dry matter digestibility (68.08%), total volatile fatty acids (35.27%), and total gas (57.4 ml/200 mg) compared to P. chrysosporium (45.09%, 32.24%, 44.39 ml/200 mg) but was second to P. ostreatus (75.34%, 38.31%, 65.60 ml/200 mg). The coculture via synergistic interaction has the potential to decompose lignin and improve the nutritive value of rice straw than P. chrysosporium.

scholarly journals PENGARUH FERMENTASI LIMBAH PADAT PENGOLAHAN BIOETANOL DARI SINGKONG (MANIHOT ESCULENTA) MENGGUNAKAN SACCHAROMYCES CEREVISIAE TERHADAP KANDUNGAN GIZI LIMBAH - Effect of Solid Waste Treatment Bioethanol Fermentation of Cassava (Manihot Esculenta) Use Saccharomyces Cerevisiae Change Nutrition Against Waste

2014 ◽  
Vol 4 (1) ◽  
Author(s):  
Yani Suryani ◽  
Iman Hernaman ◽  
Poniah Andayaningsih
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Solid Waste ◽  
Experimental Method ◽  
Manihot Esculenta ◽  
Waste Treatment ◽  
Nutrient Content ◽  
Randomized Design ◽  
Protein Fiber ◽  
Solid Waste Treatment ◽  
Completely Randomized Design

AbstrakPengolahan bioetanol yang berbahan singkong menghasilkan limbah padat yang masih jarang dimanfaatkan dan berpotensi sebagai pakan ternak. Maka sebelum diberikan pada ternak perlu dilakukan proses pengolahan melalui fermentasi. Salah satu jamur yang memiliki kemampuan dalam proses fermentasi adalah Saccharomyces cerevisiae. Penelitian ini bertujuan untuk mengetahui peningkatan kandungan nutrien limbah padat pengolahan bioetanol dari singkong (Manihot esculenta) melalui fermentasi oleh Saccharomyces cerevisiae. Penelitian ini dilakukan dengan metode eksperimental menggunakan Rancangan Acak Lengkap (RAL) pola faktorial 3 x 3 dengan ulangan tiga kali dengan dosis  inokulum (D)  masing-masing D1 = 2g, D2 = 3g dan D3 = 4g. Hasil penelitian menunjukkan bahwa peningkatan protein bervariasi dari 2% menjadi 3,05% dan penurunan kandungan kadar serat hasil fermentasi bervariasi dari 2,12%-2,36%. Kandungan HCN mengalami penurunan yang sangat signifikan dari 16,06% menjadi 0,74%. Kesimpulan bahwa fermentasi limbah padat bioetanol dengan menggunakan Saccharomyces cerevisiae dapat meningkatkan protein, menurunkan serat dan kadar HCN.Kata kunci : fermentasi, Saccharomyces cerevisiae, limbah padat bioetanol.AbstractProcessing of bioethanol made from cassava produces solid waste that is still rarely used and potentially as fodder. Therefore, before it is given to the cattle, it needs to be processed through fermentation. One of the fungi that have the ability in the process of fermentation is Saccharomyces cerevisiae. This study aims to determine the increase of nutrient content of the waste bioethanol processing from cassava (Manihot esculenta) through fermentation by Saccharomyces cerevisiae. This research was conducted with an experimental method using Completely Randomized Design (CRD) 3 x 3 factorial with three replications with a dose of inoculum (SC) respectively SC1 = 2g, 3g and SC2 = SC3 = 4g;. The results shows that increasing the protein varies from 2% to 3,05% and decreased levels of fiber content of fermented varies from 2,12% - 2,36%. The content of HCN is decreased significantly from 16,06% to 0,74%. The conclusion that solid waste bioethanol fermentation using Saccharomyces cerevisiae can increase protein, fiber and lower levels of HCN.

scholarly journals ALTERNATIVES FOR SPRING FORAGE EMPTINESS IN PAMPA, BRAZIL

2017 ◽  
Vol 4 (4) ◽  
pp. 58-64
Author(s):  
Edgard Gonçalves Malaguez ◽  
Gabriella Valduga Dinarte ◽  
Leonardo Ereno Tadielo ◽  
Nathaly Lopes Toledo Dos Santos ◽  
Deise Dalazen Castagnara
Keyword(s):  
Dry Matter ◽  
Nutritional Value ◽  
Nutrient Content ◽  
Mineral Matter ◽  
Elephant Grass ◽  
Dry Matter Digestibility ◽  
Randomized Design ◽  
Pampa Region ◽  
Completely Randomized Design

The spring forage emptiness is an obstacle to the development of Brazilian livestock, including in the Bioma Pampa region. Feed strategies for this period contributed to the development of livestock and to the conservation of the biome. The objective of this work was to know the nutritional potential of forages during spring forage for use as strategies in the forage planning. The experiment had a completely randomized design, with ten forages and four replicates. The chemical composition, in vitro dry matter digestibility (IVDMD) and the total digestible nutrient content (NTD) of elephant grass, brachiaria cultivars Convert and Marandu, Tifton 68; diploid and tetraploid ryegrass; red, white and arrowleaf clover; and bird’s-foot trefoil. The grasses had a higher proportion of fibrous constituents, and lower levels of NDT and DMD, thus, lower nutritional value. Negative correlation between mineral matter and fibrous constituents with CP, TDN and IVDMD, was observed. Forages studied can be used as pasture alternatives for ruminants in the spring forage emptiness of the Pampa, Brazil, however, the strategy of using them should be a pasture planning.

scholarly journals PENGARUH KOMPOSISI PROBIOTIK YANG BERBEDA PADA PAKAN BUATAN TERHADAP RASIO KONVERSI PAKAN DAN LAJU PERTUMBUHAN BENIH IKAN GABUS LOKAL (Channa sp) HASIL DOMESTIKASI

2021 ◽  
Vol 8 (1) ◽  
pp. 1
Author(s):  
Fazril Saputra ◽  
Yusran Ibrahim
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Growth Rate ◽  
Lactobacillus Casei ◽  
Experimental Methods ◽  
Conversion Ratio ◽  
Feed Conversion Ratio ◽  
Feed Conversion ◽  
Randomized Design ◽  
Bacillus Spp ◽  
Completely Randomized Design

Snakehead fish (Channa sp.) was one of Indonesia's native freshwater commodities that has been successfully domesticated by fish cultivators in Indonesia. However, domesticated snakehead fish cultivation still has obstacles, namely the high value of the feed conversion ratio and the low growth rate of local snakehead fish. Overcoming this problem requires a technology that can optimize the feed conversion ratio and growth rate of domestic snakehead fish. One technology that can be applied is the used of probiotic technology. This study aims to test the provision of different probiotics through feed to optimize the feed conversion ratio and growth rate of domestic snakehead fish. This research was conducted using experimental methods. The experimental design used was a completely randomized design with 4 treatments and each treatment was repeated 3 times. The treatments were treatment without probiotics / control (P0), treatment of the probiotic composition of Lactobacillus sp., Bacillus spp. and Nitrosomonas sp. (P1), treatment of the probiotic composition of Lactobacillus casei and Saccharomyces cerevisiae (P2), treatment of the probiotic composition of Lactobacillus sp., Acetobacter, Rhodobacter sp., Yeast (P3). The results showed that giving different probiotic compositions through feed on the parameters of feed conversion ratio and growth rate did not give results that were not significantly different (P> 0.05) when compared to control, but the highest treatment value was found in the probiotic composition of Lactobacillus casei and Saccharomyces cerevisiae.

Investigation of white-rot fungi for the conversion of poplar into a potential feedstuff for ruminants

1983 ◽  
Vol 29 (4) ◽  
pp. 457-463 ◽  
Author(s):  
A. E. Reade ◽  
R. E. McQueen
Keyword(s):  
Phanerochaete Chrysosporium ◽  
Nutrient Solution ◽  
Dry Matter ◽  
White Rot Fungi ◽  
White Rot ◽  
Dry Matter Yield ◽  
Ganoderma Applanatum ◽  
Practical System ◽  
Fibre Analysis

Five isolates of wood-rotting fungi were compared for their ability to increase the digestibility of poplar shavings. Homogenates of the fungi were inoculated into mixtures of 25 g of shavings and 100 mL of supplementary nutrient solution and incubated at 25 °C. The in vitro rumen digestibility of the products increased from 30% at the start of the fermentation to maxima of 72% with Polyporus anceps after 8 weeks, 64% with Ganoderma applanatum and 62% with Phanerochaete chrysosporium after 4 weeks, 61% with Polyporus versicolor after 3 weeks, and 42% with Fomitopsis ulmarius after 4 weeks. Fibre analysis showed a decrease in lignin as digestibility increased. Loss of carbohydrates occurred in all fermentations and continued after lignin loss ceased. Dry matter yield at the time of maximum digestibility ranged from 80 to 94% of the original dry matter. The highest digestibilities attained were similar to those of conventional roughage feeds for ruminants. This shows that this fermentation could form the basis of a practical system for converting poplar into a feedstuff.

scholarly journals Potensi fungi pelapuk putih asal lingkungan tropik untuk bioremediasi herbisida The potential white-rot fungi native of tropical environment for herbicides bioremediation

2016 ◽  
Vol 75 (1) ◽  
Author(s):  
Laksmita Prima SANTI ◽  
Lisdar Idwan SUDIRMAN ◽  
Didiek Hadjar GOENADI
Keyword(s):  
Laboratory Experiment ◽  
Phanerochaete Chrysosporium ◽  
White Rot Fungi ◽  
Tropical Environment ◽  
White Rot ◽  
Malt Extract ◽  
Ceriporiopsis Subvermispora ◽  
Pleurocybella Porrigens

SummaryFungal treatment by using white-rot fungito reduce a wide variety of herbicide com-pounds is a specialized bioremediation pro-cess. A laboratory experiment was conductedto determine the ability of Phanerochaetechrysosporium, Ceriporiopsis subvermispora,and Pleurocybella porrigens and seven white-rot fungi isolated from a native of tropicalenvironment to grow on yeast malt extractglucose (YMG) agar containing highconcentration of (I) 2,4-dichlorophenoxy aceticacid, (R) glyphosate, and (G) paraquat. Thedata indicated that P. chrysosporium couldgrow on YMG media containing 5000 ppm of(I) 2,4-D, whereas BPBPI 02/04 isolate onYMG 250 ppm of (R) glyphosate or (G)paraquat. Relative values of growth inhibitionof these fungi are 81.1; 27.8; and 50.0%respectively. Biodegradation capability ofherbicides by candidate inoculants in soil-sandmedia was also determined in greenhouseexperiment by using peanut, sorghum, corn,and Borreria alata as bio-indicators. Peanutand B. alata were found to be the bestresponsive seedlings as bio-indicator on thepresence of (I) 2,4-D herbicide in soil-sandmedia.RingkasanTeknologi bioremediasi dengan fungipelapuk putih (FPP) digunakan untuk me-reduksi sejumlah senyawa herbisida. Kegiatanpenelitian yang dilakukan di laboratoriumbertujuan untuk mengetahui kemampuan tum-buh Phanerochaete chrysosporium, Ceripo-riopsis subvermispora, dan Pleurocybellaporrigens serta tujuh isolat FPP yang diperolehdari lingkungan tropik secara in vitro padamedium agar yeast malt extract glucose(YMG) yang mengandung (I) 2,4-dikloro-fenoksi asam asetat, (R) glifosat, dan (G)parakuat konsentrasi tinggi. Dari data yangdiperoleh, diketahui bahwa Ph. chrysosporiummemiliki kemampuan tumbuh dalam mediumpadat YMG yang mengandung 5000 ppm (I)2,4-D dan isolat BPBPI 02/04 pada 250 ppm(R) glifosat dan (G) parakuat dengan nilaihambatan pertumbuhan relatif terhadap kontrol(HPR) masing-masing 81,1; 27,8; dan 50,0%.Pengujian isolat terpilih terhadap kemampuanmendegradasi herbisida di dalam mediumtanah dan pasir juga dilakukan di rumah kacadengan menggunakan kacang tanah, sorgum,jagung, dan Boreria alata sebagai bioindikator.Kacang tanah dan B. alata memberikan responterbaik terhadap keberadaan herbisida (I) 2,4-Ddi dalam medium tanah dan pasir .

scholarly journals Bio-Pulping of Bagasse As The Material For Paper Making Using Different Species of White Rot Fungi and Incubation Time

2020 ◽  
Vol 2 (3) ◽  
pp. 305
Author(s):  
Vio Indah Budiarti ◽  
Nuniek Ina Ratnaningtyas ◽  
Aris Mumpuni
Keyword(s):  
Incubation Time ◽  
Lignin Degradation ◽  
White Rot Fungi ◽  
White Rot ◽  
Lignin Concentration ◽  
Paper Making ◽  
Randomized Design ◽  
Independent Variable ◽  
Completely Randomized Design ◽  
Cellulose Concentration

The Biopulping is defined as the biological process of lignin degradation by utilizing microorganisms that produce some enzymes. A microorganism which widely known in the degradation of lignin and cellulose is a group of white-rot fungi. The aims for this research to know the most effective  white rot fungi species of G.lucidum, P.tuber-regium, and T.versicolor in the degradation of lignin and cellulose with different incubation time on bagasse substrate. The effectivity of biopulping indicated by the highest degradation of lignin concentration and the lowest degradation of cellulose concentration. This study used an experimental design with Completely Randomized design with a two factorial pattern. The independent variable of this study is white rot fungi species and incubation time while the dependent variable is the concentration of lignin and cellulose. The main parameter was the concentration of lignin and cellulose, supporting parameters were pH, weight loss of substrate and mycelial growth. The result showed the degradation of lignin and cellulose in all treatment. The best degradation of lignin and cellulose showed by species T.versicolor and P.tuber-regium within 30 days of incubation

scholarly journals Nutrient content evaluation of dried poultry waste urea molasses block (DPW-UMB) on In-vitro analysis

2018 ◽  
Vol 16 (2) ◽  
pp. 50
Author(s):  
Danung Nur Adli ◽  
Osfar Sjofjan
Keyword(s):  
Crude Protein ◽  
Nutrient Content ◽  
Poultry Waste ◽  
Duncan Multiple Range Test ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Vitro Analysis ◽  
Range Test ◽  
In Vitro Analysis

<p><strong><em>The research purpose was to determine nutrient content of dried of poultry waste urea molasses block (DPW-UMB). The research method was used completely randomized design with 3 treatments and 5 replicates. The treatments used for research were T1 (10% manure of laying chicken and 25% molasses), T2 (15% manure of laying chicken and 30% molasses), and T3 (20% manure of laying chicken and 30% molasses). The data analysis was the analysis of variance (anova) and continued by Duncan Multiple Range Test. The results showed that treatments has significantly difference (P&lt;0.01) on dry matter, crude protein, and ash. It could be concluded that dpw-umb contained sufficient levels of nutrients. it could be used as feedstuff for ruminants for supplementation with the required nutrients.</em></strong></p>

Aktivitas Antifungi Air Perasan Bawang Merah (Allium ascalonicum L.) Terhadap Candida albicans Secara In Vitro

2017 ◽  
Vol 9 (2) ◽  
pp. 71
Author(s):  
Nurhasanah Nurhasanah ◽  
Fauzia Andrini ◽  
Yulis Hamidy
Keyword(s):  
Candida Albicans ◽  
Antifungal Activity ◽  
Allium Sativum ◽  
Fungicidal Activity ◽  
Positive Control ◽  
Negative Control ◽  
Randomized Design ◽  
Significant Difference ◽  
Completely Randomized Design

Shallot (Allium ascalonicum L.) has been known as traditional medicine. Shallot which has same genus with garlic(Allium sativum L.) contains allicin that is also found in garlic and has been suspected has fungicidal activity toCandida albicans. It is supported by several researches. Therefore, shallot is suspected has antifungal activity too.The aim of this research was to know antifungal activity of shallot’s water extortion againsts Candida albicans invitro. This was a laboratory experimental research which used completely randomized design, with diffusion method.Shallot’s water extortion was devided into three concentrations, there were 50%, 100% and 200%. Ketoconazole 2%was positive control and aquadest was negative control. The result of this research based on analysis of varians(Anova), there was significant difference between several treatments and was confirmed with Duncan New MultipleRange Test (DNMRT) p<0,05, there was significant difference between 100% shallot’s water extortion with othertreatments, but there was no significant difference between 50% shallot’s water extortion with 200% shallot’s. Theconclusion was shallot’s water extortion had antifungal activity againsts Candida albicans with the best concentration100%, but it was lower than ketoconazole 2%.

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